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... A kind of 1,2-dithioglycol (DTG) functionalised carbon nitride quantum dots (DTG-CNQDs) was designed for the first time by modifying DTG on the surface of carbon nitride quantum dots (CNQDs). The as-prepared DTG-CNQDs exhibit strong blue fluorescence under ultraviolet light and have a high quantum yield of 27%. Experiments show that Hg²⁺ has a good quenching effect on the fluorescence of DTG-CNQDs ...
... Conventional descriptive analysis (DA) has been widely applied to describe the sensory characteristics of products. An analysis of the scientific literature was performed to better understand the context in which descriptive methods are utilized. When DA is used in methodological research, either to test the method itself, or to compare to new methods, is that representative of its use in applicat ...
... 3D printing can significantly improve the current fabrication techniques for microfluidic devices due to its ability to create truly 3D structures in a single step. In this study, an active pneumatic microfluidic mixer was designed and fabricated using an extrusion-based 3D printer and used for rapid detection of . The printed material of the mixer is flexible, semi-transparent, and inexpensive. T ...
... Escherichia coli O157:H7 bacterium as a zoonotic pathogen is one of the most important causative agents of foodborne illnesses worldwide. Due to the serious concerns in public health and enormous economic losses in agriculture and food industry, it is very necessary to develop novel technology–based methods for sensitive and rapid detection of this bacterium in contaminated resources. In this stud ...
... A novel colorimetric nanosensor has been developed for high sensitive and selective detection of cysteine (Cys) based on the intrinsic oxidase-like activity of cerium nanotube coordinated with aspartic acid (Asp/Ce-NT). Under the function of Asp/Ce-NT, colorimetric reaction of TMB (3,3′,5,5′-tetramethylbenzidine) takes place within five minutes without the assistance of additional oxidizing agents ...
... C-reactive protein (CRP) is one of the important indicators of inflammatory response. It plays as an essential role in diseases such as myocardial infarction and atherosclerosis. A simple and effective label-free electrochemiluminescent (ECL) immunosensor for quantitative detection of CRP is reported. The indium tin oxide coated glass was prefunctionalized with the composite of titania nanotubes a ...
artificial intelligence; calorimetry; canned fish; colorimetry; histamine; marine fish; mobile telephones; monitoring; rapid methods; scombroid poisoning; temperature
Abstract:
... Self-assembled polydiacetylene (PDA) vesicles, with the distinct advantages of low-cost materials, simple preparation, and excellent chromatic properties, can be perfectly combined with a colorimetric strip for on-site inspection. Herein, without involving expensive reagents and instruments, a visual colorimetric strip based on well-prepared PDA vesicles was developed to analyze and monitor histam ...
... Cydia pomonella granulovirus, in particular CpGV-M isolate, is used as a biological control against the codling moth (CM), Cydia pomonella. As a result of intensive control over the years, codling moth populations have developed resistance against this isolate. This resistance is now called type I resistance. Isolates, among them, CpGV-R5, have been found that are able to overcome type I resistanc ...
biogeochemistry; cameras; color; colorimetry; digital images; ecosystems; hydric soils; hydrology; mobile telephones; photography; rapid methods; soil color; spectroscopy; wetlands
Abstract:
... Soil color patterns are essential to understand hydrologic regime and biogeochemical processes in wetland ecosystems. Munsell Soil Color Chart (MSCC) has been traditionally and predominantly used to identify and quantify hydric soil field indicators, but several simple, low-cost alternatives have become recently available to compare their efficacy in complementing the MSCC in soil color assessment ...
... Sensors based on graphene are promising devices for chemical and biological detection owing to their high sensitivity, biocompatibility, and low costs. However, for chiral recognition, which is very important in biological systems, graphene sensors remain unable to discriminate enantiomers. Here, using chiral pesticide molecules as an example, we realized a highly sensitive graphene chiral sensor ...
... With the increasing of the food safety awareness, the quality control of amaranth as the common food additives was significant for human’s physical health. The objective of this study was to establish a green, simple, and rapid fluorescence sensor for amaranth quantification detection. The nitrogen-doped graphene quantum dots (NGQDs) were synthesized under a friendly environment and non-toxic mate ...
... Current developments in meta-data analysis and predictive computational models offer alternative routes for the identification of antibodies. In silico-based technologies and NGS data analysis from Ab phage-display selections offer expanded selections of Ab candidates. Accordingly, the identified de novo Abs with predicted selectivity for a target antigen must undergo rapid gene synthesis for down ...
... The paper presents application of a new three‐parameter method for identification of volatile organic compounds (VOCs) and creation of fingerprints based on the impregnation time (tIM), cleaning time (tCL), and maximum response ([ΔR/R]ₘₐₓ) of chemically sensing sensors for detecting spoilage of agricultural commodities. The novelty of this method consists in the use of two additional parameters: a ...
... H₂S is an important signaling molecule in living systems and participates in a series of physiological and pathological processes. In situ and rapid detection of H₂S levels in living systems is of great importance to the in-depth study of its physiological and pathological roles. Herein, we present a new fluorescent probe (DFAN) for the detection of H₂S in live cells and zebrafish. The probe DFAN ...
... We report the development of a Lab-on-PCB DNA diagnostic platform, exploiting peptide nucleic acid (PNA) sequences as probes. The study demonstrates the optimization and characterization of two commercial PCB manufacturing gold electroplating processes for biosensing applications. Using an optimized ratio of PNA with a spacer molecule (MCH), the lowest limit of detection (LoD) to date for PCB-base ...
... Abnormal protein concentration levels in human body fluids, such as urine, serum etc., are considered to associate with disease states, providing essential information for the pre-clinical diagnosis. This paper presents a wireless immunoglobulin-coated magnetoelastic (ME) biosensor for fast, cost-effective detections of human serum albumin (HSA) with small sample volumes at a microliter scale. Thi ...
aquifers; buildings; cities; data collection; digital elevation models; dynamic models; ecosystems; government agencies; groundwater; human health; infiltration rate; infrastructure; planning; rapid methods; risk; sea level; shorelines; soil; stormwater; subsidence
Abstract:
... Sea level rise (SLR) will cause shallow unconfined coastal aquifers to rise. Rising groundwater can emerge as surface flooding and impact buried infrastructure, soil behavior, human health, and nearshore ecosystems. Higher groundwater can also reduce infiltration rates for stormwater, adding to surface flooding problems. Levees and seawalls may not prevent these impacts. Pumping may accelerate lan ...
aquiclude; aquifers; databases; geographic information systems; geophysical logging; geophysics; groundwater; hydraulic conductivity; rapid methods; water management; Illinois
Abstract:
... The needs of subregional and local entities for water resource management are immediate, and required information is not forthcoming in a timely and relatively inexpensive manner. The following method allows one to use all available well record data to produce baseline hydrogeologic unit maps using geographic information system querying and mapping techniques. Subjective borehole and geophysical s ...
... Overlay plate counting is the traditional way to determine the biomass of viable Acidithiobacillus ferrooxidans in iron-based bioleaching matrix of sewage sludge. However, the time required is 12 days at a minimum. Here, a rapid method based on a quantitative relationship between the extant rate of iron bio-oxidation by Acidithiobacillus ferrooxidans and the CFU counts was developed. The method re ...
... Sensitive yet rapid methods for detection of rare earth elements (REEs), including lanthanides (Lns), would facilitate mining and recycling of these elements. Here we report a highly selective, genetically encoded fluorescent sensor for Lns, LaMP1, based on the recently characterized protein, lanmodulin. LaMP1 displays a 7-fold ratiometric response to all Lnᴵᴵᴵs, with apparent Kds of 10–50 pM but ...
... Sulfonation degree is an important structural parameter of lignosulfonates which affect their water solubility, surface activity, and dispersing performance. Presently, the methods for determining sulfonation degree include elemental analysis and potentiometric titration; they have some shortcomings such as complex purification process, high equipment requirements, and time-consuming. Hence, a sim ...
... Multiple catchment controls contribute to the geomorphic functioning of river systems at the reach-level, yet only a limited number are usually considered by river scientists and managers. This study uses multiple morphometric, geological, climatic and anthropogenic catchment characteristics to produce a single national typology of catchment controls in England and Wales. Self-organising maps, a m ...
... BACKGROUND: Plants rely on concentration gradients of the native auxin, indole-3-acetic acid (IAA), to modulate plant growth and development. Both metabolic and transport processes participate in the dynamic regulation of IAA homeostasis. Free IAA levels can be reduced by inactivation mechanisms, such as conjugation and degradation. IAA can be conjugated via ester linkage to glucose, or via amide ...
... Early screening of foodborne pathogenic bacteria is a key to prevent and control foodborne diseases. This study intended to develop a capillary biosensor for rapid and sensitive detection of Salmonella using the multi-column capillary for easy operation, the Fe-nanoclusters (FNCs) for signal amplification and the smart phone APP for image analysis. The multi-column capillary was successively prelo ...
... Cell viability evaluation is very meaningful for cancer treatment and cell proliferation is an effective evaluation criterion for cell viability. Traditionally, cell proliferation rate is obtained only by monitoring for several cell cycles (about 12 h) and yet there is no rapid assessment method to evaluate cell proliferation. In this paper, a rapid, real-time and online assessment approach (about ...
... The rapid detection of Salmonella typhi can be accomplished by developing nanobiosensor kits. Accordingly, in the present study, a molecularly imprinted polymer (MIP) was developed, formed via covalent bonds among methacrylic acid (MAA) monomers and hydrogen bonds between MAA and antibodies. Using this MIP, S. typhi antigen was detected with a fluorescence converter. The S. typhi concentration cou ...
... In many occasions it is necessary to use fast and simple methods, different to the chromatographic techniques, for the quantification of biomolecules such as flavonoids. Also, the flavonoid levels in some foodstuffs can be influenced by industrial extraction processes such as pressing and squeezing, resulting in modification of their functional value. For this purpose, we have developed a rapid me ...
... In this study, a sensitive monoclonal antibody (mAb) against toltrazuril (Tol) was developed based on a novel hapten. The 50% inhibitory concentrations (IC50) of toltrazuril and its metabolites ranged from 2.19 ng/mL to 4.21 ng/mL. Based on this mAb, a colorimetric paper-based sensor was developed for the rapid screening of Tol and its metabolites in samples. The proposed assay has cutoff values o ...
... Infection with parasites from the Trichinella genus occurs in many vertebrates but disease only occurs in humans (trichinellosis). Humans are infected after the consumption of raw or undercooked meat from infected wild or domestic animals (usually swine or horses). Using the monoclonal antibody (mAb) 7C2C5, specific for an epitope unique to the muscle larvae of the genus Trichinella, we have devel ...
... A novel competitive electrochemical immunosensor based on Au nanodendrites (Au NDs)/silver nanoparticles (Ag NPs) @single-walled carbon nanohorns (SWCNHs) was established for sensitive determination of sulphonamides (SAs) in aquatic environments. The indirectly competitive binding system of the approach was composed of coating antigen that coated on Au NDs/glass carbon electrode (GCE), the target ...
chlorpyrifos; cleaning; cortex; gas chromatography; ionization; medicinal plants; rapid methods; secondary metabolites; seeds; semen; spectrometers; tandem mass spectrometry
Abstract:
... Developing an analysis of multi-pesticide residues for different herbal species-ready applications is a challenge. In the present work, a comprehensive analysis was proposed for rapid detection of 201 pesticides in various medicinal herbs. Samples were extracted and cleaned up with a high throughput pretreatment approach (modified QuEChERS), and then detected by gas chromatograph coupled to an ele ...
... Turmeric (Curcuma longa) is one of the most important ingredients in Indian and Chinese cuisine. Curcuminoids and volatile oils present in turmeric are known for their functional and nutraceutical properties. Health benefits attributed to curcuminoids have resulted in their wide utilization in food and pharmaceutical formulations. Therefore, characterization and estimation of the curcuminoids in f ...
... We report a novel diffuse reflectance-Fourier transforms infrared (DRS-FTIR) spectral monitoring of fluoroquinolone antibiotics such as ciprofloxacin (CIP) and norfloxacin (NOR) for their quantification in poultry egg-yolks. To the best of our knowledge, this is the first report to describe the rapid quantitative determination of fluoroquinolone antibiotics in poultry egg samples using DRS-FTIR. T ...
Lewis bases; chemical bonding; colorimetry; emissions; fluorescence; fluorescent dyes; image analysis; mass spectrometry; membrane permeability; moieties; nuclear magnetic resonance spectroscopy; rapid methods; sulfur dioxide
Abstract:
... A novel mitochondria-targeted fluorescent probe for sensing SO₂ derivatives was constructed by integrating phenanthroimidazole and cyanine moieties. Excited by different channels, this probe exhibited unique dual-channel fluorescence-enhanced emissions and colorimetric response to HSO₃⁻ quickly (less than 20 s). The sensing process was confirmed to undergo nucleophilic addition of HSO₃⁻ to C˭C dou ...
... In this study, water-soluble citrate-capped copper nanoparticles (Cu NPs) were synthesized by a simple and rapid method. We found that these citrate-capped Cu NPs possessed an intrinsic peroxidase-like activity, which could catalyse the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a light blue product in the presence of hydrogen peroxide (H₂O₂). It was also found that mercury ions ...
... In this study, a simple and rapid method was designed to synthesize N-doped silicon quantum dots (N-SiQDs) with a maximum excitation wavelength and maximum emission wavelength of 347 nm and 440 nm, respectively. The prepared N-SiQDs are colorless in aqueous solution with excellent dispersibility, and the fluorescence quantum yield is 28.8%. The fluorescence intensity of the N-SiQDs decreases rapid ...
activation energy; crosslinking; detection limit; electrophoresis; gases; gel chromatography; hyaluronic acid; molecular weight; monitoring; prototypes; rapid methods; temperature
Abstract:
... We report a facile, high-resolution approach to quantitatively characterize hyaluronic acid (HA) and study its crosslinking reaction using electrospray-differential mobility analysis (ES-DMA). Mobility size distributions, number concentrations, molecular mass distributions, and polydispersity index of HAs were obtained successfully via a rapid analysis by ES-DMA (< 30 min). The limit of detection, ...
... By combination of miniaturization potential of digital microfluidics (DMF) and sensitivity of fluorescence probe, an integrated sensor device has been initially constructed for mercury detection in coastal waters. The actuation feature of the detecting target, seawater droplet, which remains unclear, was basically explored. To overcome a potential risk of driven failure, induced by diversity ion i ...
DNA probes; chemical reactions; graphene oxide; microRNA; nanoparticles; neoplasms; photoluminescence; polyurethanes; porous media; rapid methods; silica; single-stranded DNA
Abstract:
... We have developed a photoluminescent membrane for microRNA detection, consisting of chemically modified mesoporous silica nanoparticles (CaF₂:Yb/Ho@MSNs) attached, via single stranded DNA probes, to flexible polyurethane fibres coated with graphene oxide (GO). By detecting the release of the luminescent nanoparticles resulting from complementary co-hybridization between target miRNA sequences and ...
... A fluorescence immunoassay has been widely applied in different fields due to its high sensitivity, simple operations, and high accuracy. Quantum dots (QDs) are often selected as labels in a fluorescence immunoassay due to their high fluorescence, better stability, and biocompatibility. In this study, novel doped CdTe : Zn/ZnS QDs with stability and a high photoluminescence quantum yield (40.78%) ...
... We designed a rapid method for DNA sensing for detection of campylobacter jejuni based on a selective DNA probe. A sequence containing 12 cytosine nucleotides was applied as a scaffold for making Au/Ag nanocluster. This sequence along with a 30 nucleotides selective probe was used for campylobacter jejuni DNA detection. The Au/Ag nanocluster showed a turn-off fluorescence response in presence of t ...
... New generation of toxicological tests and assessment strategies require validated toxicokinetic data or models that are lacking for most chemicals. This study aimed at developing a quantitative property-property relationship (QPPR)-based human physiologically based pharmacokinetic (PBPK) modeling framework for high-throughput predictions of inhalation toxicokinetics of organic chemicals. A PBPK mo ...
... Point‐of‐care detection for pathogen is of critical need for wide epidemic warning and medical diagnosis. In this work, we have designed and developed a fully portable and integrated microchip based real‐time polymerase chain reaction machine for rapid pathogen detection. The instrument consists of three functional components including heating, optical, and electrical modules, which are integrated ...
... Rapid assessment of spatial distribution of earthquake-induced landslides could provide valuable information in the emergency response phase. Previous studies proposed global analyses with the aim of predicting earthquake-induced landslide distributions in near real-time. However, in all those studies, mapping units are constituted by pixels, which do not reflect homogeneously distributed physical ...
David G. Buck; David C. Evers; Evan Adams; Joseph DiGangi; Bjorn Beeler; Jan Samánek; Jindrich Petrlik; Madeline A. Turnquist; Olga Speranskaya; Kevin Regan; Sarah Johnson
... We present data on a rapid assessment of fish Hg concentrations from 40 different waterbodies in 26 countries that includes data on 451 fish of 92 species. Significant differences in fish Hg concentrations were observed across fish foraging guilds and in general, higher trophic level fish (i.e., piscivores and carnivores) showed the highest mean total Hg (THg) concentrations. However, elevated THg ...
... BACKGROUND: Guayule (Parthenium argentatum A. Gray), a plant native to semi-arid regions of northern Mexico and southern Texas in the United States, is an alternative source for natural rubber (NR). Rapid screening tools are needed to replace the current labor-intensive and cost-inefficient method for quantifying rubber and resin contents. Near-infrared (NIR) spectroscopy is a promising technique ...
DNA; derivatization; disease diagnosis; fluorescence; graphene; human serum albumin; metabolites; nucleotide aptamers; rapid methods; sarcosines; systematic evolution of ligands by exponential enrichment; urea; urine
Abstract:
... Sarcosine is an amino acid derivative, which is considered as a key metabolite in various metabolic processes. Therefore, simple and sensitive detection methods are needed for further understanding its metabolic role and diagnostic value. In this study, we developed a novel method that meets the need for practical and sensitive detection in a complex medium mimicking urine conditions. For this aim ...
Rubus idaeus; anti-obesity agents; dissociation; farms; food additives; high performance liquid chromatography; ionization; metabolomics; quality control; rapid methods; raspberries; tandem mass spectrometry; ultra-performance liquid chromatography
Abstract:
... Raspberry ketone (RK) is the characteristic aromatic compound in raspberry (Rubus idaeus L.) with wide applications as food additive and anti-obesity agent. However, quantification of RK has presented difficulties in MS detection and reliable LC-MS method for RK analysis in literature is in limit to date. In order to facilitate quality control of raspberry derived products and RK metabolomics stud ...
Huan FengThese authors contributed equally to this work and they should be regarded as co-first authors.; Yue Wang; Jianping Liu; Zhiqiang Zhang; Xinyi Yang; Rong Chen; Qingtao Meng; Run Zhang
... Hypochlorous acid (HOCl) is closely related to human health and is widely used in our daily lives. Accordingly, detection of HOCl in live organisms and natural water samples is significantly important to human and animal health. In this paper, a novel chromogenic and fluorogenic probe (PI) was prepared for HOCl detection with high sensitivity and specificity. PI exhibits weak fluorescence emission ...