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- Dou, Tongyi, et al. Show all 8 Authors
- Chemical communications 2019 v.55 no.30 pp. 4311-4314
- catechol O-methyltransferase; chemical reactions; ligands; lysine; mass spectrometry; prediction; receptors
- ... Herein we provide a mass spectrometry-based lysine reactivity profiling strategy to monitor the ligand modulation of protein receptors, which is achieved by active dimethyl labeling of lysine residues and comparison of the alterations of labeling reactivity during ligand binding. The small-molecule ligand modulation patterns on the catechol-O-methyltransferase and the N-methyl-d-aspartate receptor ...
- Dou, Tongyi, et al. Show all 10 Authors
- Analytical chemistry 2019 v.91 no.9 pp. 5638-5645
- Danio rerio; biotransformation; carboxylesterase; cell membranes; fluorescence; fluorescent dyes; humans; hydrolysis; optical properties; screening; substrate specificity
- ... Rational design of practical probes with excellent specificity and improved optical properties for a particular enzyme is always a big challenge. Herein, a practical and highly specific fluorescent probe for carboxylesterase 1 (CES1) was rationally designed using meso-carboxyl-BODIPY as the basic fluorophore based on the substrate preference and catalytic properties of CES1. Following molecular do ...