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1. Does genetic diversity of grass improve yield, digestibility, and resistance to weeds, pests and disease infection?

Author:
Isogai, Taiki,   et al.   ; Yoshihara, Yu;     Show all 2 Author
Source:
Archiv für Acker- und Pflanzenbau und Bodenkunde 2019 v.65 no.12 pp. 1623-1629
ISSN:
1476-3567
Subject:
agricultural land; cultivars; disease control; dry matter digestibility; ecological function; farmers; fertilizer application; genetic variation; grasses; insects; leaves; mixed culture; mixing; net primary productivity; pest control; pollination; soil fertility; weeds
Abstract:
... Intraspecies genetic level diversity has the potential to improve ecosystem functions and services, similar to that by species-level diversity. Although yield, pollination, and pest and disease control have been enhanced by crop genetic diversity, mixing multiple cultivars of grass within a species in an agricultural field have not been fully tested by farmers. We, therefore, tested whether multip ...
DOI:
10.1080/03650340.2019.1566719

2. Cell-free DNA in blood circulation is generated by DNase1L3 and caspase-activated DNase

Author:
Watanabe, Taiki,   et al.   ; Takada, Shuhei; Mizuta, Ryushin;     Show all 3 Authors
Source:
Biochemical and biophysical research communications 2019 v.516 no.3 pp. 790-795
ISSN:
0006-291X
Subject:
DNA; acetaminophen; antibodies; apoptosis; biomarkers; blood circulation; deoxyribonucleases; genes; mice; monitoring; necrosis; neoplasms; overdose; screening
Abstract:
... Cell-free DNA (cfDNA) (e.g. fetal- or tumor-derived DNA) is DNA found in the blood circulation. It is now widely investigated as a biomarker for prenatal screening, tumor diagnosis, and tumor monitoring as “liquid biopsies”. However, the biological and biochemical aspects of cfDNA remain unclear. Although cfDNA is considered to be mainly derived from dead cells, information is scarce as to whether ...
DOI:
10.1016/j.bbrc.2019.06.069

3. 4-Bromo-1,8-naphthalimide derivatives as fluorogenic substrates for live cell imaging of glutathione S-transferase (GST) activity

Author:
Nampo, Taiki,   et al.   ; Fujikawa, Yuuta; Terakado, Kenta; Mori, Masaya; Inoue, Hideshi;     Show all 5 Authors
Source:
Talanta 2019 v.204 pp. 633-640
ISSN:
0039-9140
Subject:
drug therapy; enzyme activity; fluorescence; genetic markers; glutathione transferase; humans; image analysis; isozymes; moieties; neoplasm cells; neoplasms; tissues
Abstract:
... Fluorogenic substrates are used to visualize the activity of cancer-associated enzymes and to interpret biological events. Certain types of glutathione S-transferase (GST), such as Pi class GST (referred to as GSTP1), are more highly expressed in a wide variety of human cancer tissues compared to their corresponding normal tissues. Pi class GST is thus a cancer cell molecular marker and potential ...
DOI:
10.1016/j.talanta.2019.06.059

4. Effects of phosphorus and nitrogen fertilization on soil arylsulfatase activity and sulfur availability of two tropical plantations in southern China

Author:
Mori, Taiki,   et al.   ; Wang, Senhao; Zhou, Kaijun; Mo, Jiangming; Zhang, Wei;     Show all 5 Authors
Source:
Forest ecology and management 2019
ISSN:
0378-1127
Subject:
arylsulfatase; enzyme activity; fertilizer application; forest plantations; microbial biomass; microorganisms; nitrogen; nitrogen fertilizers; nutrient management; nutrients; phosphorus; phosphorus fertilizers; soil pH; sulfur; tropical forests; tropical plants; China
Abstract:
... Understanding how phosphorus (P) and nitrogen (N) fertilization influence the dynamics of other nutrients [particularly sulfur (S)] is essential for improving the integrated nutrient management of tropical tree plantations. This study investigated how adding P and N fertilizers would affect S dynamics in the soils of two tropical forest plantations. Specifically, we tested whether the addition of ...
DOI:
10.1016/j.foreco.2019.117613

5. Natriuretic peptide signaling is involved in the expression of oxidative metabolism-related and muscle fiber constitutive genes in the gastrocnemius muscle

Author:
Hara, Taiki,   et al.   ; Ishikawa, Kiyoshi; Mizukawa, Mao; Fukano, Yasufumi; Shimomura, Takeshi;     Show all 5 Authors
Source:
Molecular and cellular endocrinology 2019 v.494 pp. 110493
ISSN:
0303-7207
Subject:
aerobiosis; cyclic GMP; gene expression; gene expression regulation; genes; high fat diet; mice; muscle fibers; muscles; myosin heavy chains; natriuretic peptide receptors; natriuretic peptides; obesity; oxygen consumption; skeletal muscle
Abstract:
... Natriuretic peptides regulate cyclic guanosine monophosphate (cGMP) levels via their receptors and have various physiological effects. Natriuretic peptide receptor C (NPR-C) increases cGMP signaling by functioning as a clearance receptor. We analyzed the role of natriuretic peptides in the skeletal muscle, which increases in mass with bone elongation, of NPR-C− mice. High-fat diet (HFD)-fed NPR-C− ...
DOI:
10.1016/j.mce.2019.110493

6. A potential source of soil ecoenzymes: From the phyllosphere to soil via throughfall

Author:
Mori, Taiki,   et al.   ; Wang, Senhao; Zhang, Wei; Mo, Jiangming;     Show all 4 Authors
Source:
Applied soil ecology 2019 v.139 pp. 25-28
ISSN:
0929-1393
Subject:
phyllosphere; rain; soil; soil organic matter; throughfall; tropical forests
Abstract:
... Soil ecoenzymes play an important role in the decomposition and formation of soil organic matter, and are often considered to be derived from soil microbial and plant root activities. This study found another potentially-important source, transfer of ecoenzymes in phyllosphere to soil via throughfall. Throughfall samples were collected in five subtropical forests, and six ecoenzymes were determine ...
DOI:
10.1016/j.apsoil.2019.02.004

7. Emergence of apple bacterial quick decline caused by Dickeya dadantii in Japan

Author:
Nakamura, Taiki,   et al.   ; Fujikawa, Takashi; Ota, Nobuyoshi; Sasaki, Makoto; Iwanami, Toru;     Show all 5 Authors
Source:
Journal of general plant pathology 2019 v.85 no.4 pp. 314-319
ISSN:
1345-2630
Subject:
Dickeya dadantii; apples; decline; phenotype; tissues; Japan
Abstract:
... Since 2014 in some parts of Iwate Prefecture in Japan, an apple disease called quick decline (QD) has been reported. The causal agent was isolated from tissues with visible QD symptoms and identified as the soft-rot pathogen Dickeya dadantii by morphological, physiological, biochemical, phenotypic and genetic characterizations. To the best of our knowledge, this is the first report of apple QD dis ...
DOI:
10.1007/s10327-019-00852-y

8. Differences in larval growth of Pacific bluefin tuna (Thunnus orientalis) between two spawning areas, and an evaluation of the growth-dependent mortality hypothesis

Author:
Ishihara, Taiki,   et al.   ; Watai, Mikio; Ohshimo, Seiji; Abe, Osamu;     Show all 4 Authors
Source:
Environmental biology of fishes 2019 v.102 no.4 pp. 581-594
ISSN:
0378-1909
Subject:
Thunnus orientalis; chlorophyll; larvae; larval development; mortality; ontogeny; spawning; surface water temperature; swimming; Sea of Japan
Abstract:
... The larval growth of Pacific bluefin tuna (Thunnus orientalis) was compared between two main spawning areas, the Sea of Japan (SOJ) and the western North Pacific (WNP). Growth trajectories indicate that the larval survival depends on their growth in both areas. Until the flexion stage, larvae of the SOJ showed lower growth than those of the WNP, but after the post-flexion stage, there was no signi ...
DOI:
10.1007/s10641-019-00855-w

9. Bicelle-to-Vesicle Transition of a Binary Phospholipid Mixture Guided by Controlled Local Lipid Compositions: A Molecular Dynamics Simulation Study

Author:
Shigematsu, Taiki,   et al.   ; Koshiyama, Kenichiro; Taneo, Masaki; Wada, Shigeo;     Show all 4 Authors
Source:
TheJournal of physical chemistry 2019 v.123 no.14 pp. 3118-3123
ISSN:
1520-5207
Subject:
encapsulation; lipid composition; molecular dynamics; phosphatidylcholines; simulation models; sonication
Abstract:
... An essential step of nanoliposome formation in an aqueous lipid solution is the transition from discoidal lipid aggregate (bicelle) to vesicle. We investigate here the bicelle-to-vesicle transition of a binary lipid mixture of saturated and unsaturated phosphatidylcholine by performing nonequilibrium molecular dynamics simulations with the coarse-grained representation of di-palmitoyl-phosphatidyl ...
DOI:
10.1021/acs.jpcb.8b10682

10. Site-selective conversion of azido groups at carbonyl α-positions into oxime groups leading triazide to a triple click conjugation scaffold

Author:
Yokoi, Taiki,   et al.   ; Ueda, Tomomi; Tanimoto, Hiroki; Morimoto, Tsumoru; Kakiuchi, Kiyomi;     Show all 5 Authors
Source:
Chemical communications 2019 v.55 no.13 pp. 1891-1894
ISSN:
1364-548X
Subject:
chemical reactions; diazo compounds; nitrogen; oximes
Abstract:
... This paper reports the selective conversion of alkyl azido groups at the carbonyl α-position into oximes through β-elimination of dinitrogen, followed by transoximation. With this method and diazo conversion, a triazido molecule was transformed into a triple click conjugation scaffold allowing one-pot four-component coupling. ...
DOI:
10.1039/c8cc09415b

11. Potential Dependence of Electronic Transition Spectra of Interfacial Ionic Liquids Studied by Newly Developed Electrochemical Attenuated Total Reflectance Spectroscopy

Author:
Sato, Taiki,   et al.   ; Tanabe, Ichiro; Suyama, Aki; Fukui, Ken-ichi;     Show all 4 Authors
Source:
Analytical chemistry 2019 v.91 no.5 pp. 3436-3442
ISSN:
1520-6882
Subject:
absorbance; anions; cations; electric potential; electrochemistry; electrodes; hysteresis; ionic liquids; reflectance spectroscopy; semiconductors
Abstract:
... Recently, ionic liquids at the electrode/ionic liquid interface have been intensively studied because they are promising as novel alternatives to traditional electrolyte solutions that are both safe and functional. In this study, we constructed an attenuated total-reflectance spectroscopic system that operates under electrochemical conditions in order to investigate the electronic states of ionic ...
DOI:
10.1021/acs.analchem.8b04931

12. Effect of pepA deletion and overexpression in Aspergillus luchuensis on sweet potato shochu brewing

Author:
Futagami, Taiki,   et al.   ; Setoguchi, Sho; Mizutani, Osamu; Yamada, Osamu; Iwai, Kenichi; Takase, Yoshikazu; Tamaki, Hisanori;     Show all 7 Authors
Source:
Journal of bioscience and bioengineering 2019 v.128 no.4 pp. 456-462
ISSN:
1389-1723
Subject:
Aspergillus; amino acid composition; breeding; citric acid; distilled spirits; enzyme activity; koji; mash; odors; pH; proteinases; rice; sweet potatoes
Abstract:
... The mash of sweet potato shochu (Japanese distilled spirit) has a low pH value because the shochu koji mold produces a large amount of citric acid, which prevents germ contamination. In this study, we examined acid protease PepA's role in shochu production. For this purpose, we constructed pepA deletion and overexpression strains, using a black koji mold Aspergillus luchuensis RIB 2604 (NBRC 4314) ...
DOI:
10.1016/j.jbiosc.2019.03.019

13. Non-aerated single-stage nitrogen removal using a down-flow hanging sponge reactor as post-treatment for nitrogen-rich wastewater treatment

Author:
Kataoka, Taiki,   et al.   ; Tanikawa, Daisuke; Yamashita, Sumire; Sonaka, Hideaki; Hirakata, Yuga; Hatamoto, Masashi; Yamaguchi, Takashi;     Show all 7 Authors
Source:
Chemosphere 2019 v.233 pp. 645-651
ISSN:
0045-6535
Subject:
Amaricoccus; Nitrosovibrio; Pseudoxanthomonas; Xanthobacter; aerobic digestion; ammonia; anaerobic digestion; carbon; chemical oxygen demand; denitrification; denitrifying bacteria; laboratory experimentation; nitrification; nitrifying bacteria; nitrogen; nitrogen content; nitrogen-fixing bacteria; rubber; rubber industry; sludge; sodium acetate; wastewater; wastewater treatment; South East Asia
Abstract:
... A laboratory-scale experiment is conducted to remove nitrogen from nitrogen-rich wastewater using a down-flow hanging sponge (DHS) reactor. Effluent from an anaerobic–aerobic system for treating synthetic natural rubber wastewater, which still contains high levels of ammonia, was used as nitrogen-rich wastewater. Experimental period was divided into four phases based whether a carbon source was fe ...
DOI:
10.1016/j.chemosphere.2019.06.012

14. Essential and distinct roles of phosphatidylinositol 4-kinases, Pik1p and Stt4p, in yeast autophagy

Author:
Futagami, Taiki,   et al.   ; Kurokawa, Yuna; Konishi, Rikako; Yoshida, Akane; Tomioku, Kanna; Tamaki, Hisanori; Tanabe, Kenji; Fujita, Akikazu;     Show all 8 Authors
Source:
Biochimica et biophysica acta 2019 v.1864 no.9 pp. 1214-1225
ISSN:
1388-1981
Subject:
autophagy; cytosol; eukaryotic cells; lipids; mammals; mutants; phosphotransferases (kinases); temperature; vacuoles; yeasts
Abstract:
... Autophagy is a degradative cellular pathway that protects eukaryotic cells from starvation/stress. Phosphatidylinositol 4-kinases, Pik1p and Stt4p, are indispensable for autophagy in budding yeast, but participation of PtdIns-4 kinases and their product, phosphatidylinositol 4-phosphate [PtdIns(4)P], is not understood. Nanoscale membrane lipid distribution analysis showed PtdIns(4)P is more abunda ...
DOI:
10.1016/j.bbalip.2019.05.004

15. Malnutrition is Associated with Behavioral and Psychiatric Symptoms of Dementia in Older Women with Mild Cognitive Impairment and Early-Stage Alzheimer’s Disease

Author:
Sugimoto, Taiki,   et al.   ; Kimura, Ai; Kitamori, Kazuya; Saji, Naoki; Niida, Shumpei; Toba, Kenji; Sakurai, Takashi;     Show all 7 Authors
Source:
Nutrients 2019 v.11 no.8
ISSN:
2072-6643
Subject:
Alzheimer disease; aggression; analysis of covariance; at-risk population; cognition; cognitive disorders; elderly; factor analysis; malnutrition; memory; nutrition assessment; nutritional status; patients; women
Abstract:
... We examined the nutritional status and its association with behavioral psychiatric symptoms of dementia (BPSD) among 741 memory clinic patients (normal cognition (NC), 152; mild cognitive impairment (MCI), 271; early-stage Alzheimer disease (AD), 318). Nutritional status and BPSD were assessed using the Mini Nutritional Assessment Short-Form (MNA-SF) and the Dementia Behavior Disturbance Scale (DB ...
DOI:
10.3390/nu11081951

16. ‘Lipase and protease production of dairy Penicillium sp. on milk‐protein‐based solid substrates’

Author:
Machiya, Taiki,   et al.   ; Kumura, Haruto; Satoh, Megumi; Hosono, Makoto; Hayakawa, Toru; Wakamatsu, Jun‐ichi;     Show all 6 Authors
Source:
International journal of dairy technology 2019 v.72 no.3 pp. 403-408
ISSN:
1364-727X
Subject:
Penicillium roquefortii; culture media; enzyme activity; imitation cheese; isozymes; lipolysis; pH; proteinases; proteolysis; triacylglycerol lipase; whey
Abstract:
... The lipolytic and proteolytic activity of Penicillium camemberti PC TT033 and Penicillium roqueforti PR G3, cultured on the whey solids or simulated cheese media, were compared under several pH reaction conditions. Lipolytic activity was higher when both strains had been cultured on the whey medium than on the simulated cheese medium, whereas proteolytic activity was less influenced by the culture ...
DOI:
10.1111/1471-0307.12597

17. Effective and Intact Cell Detachment from a Clinically Ubiquitous Culture Flask by Combining Ultrasonic Wave Exposure and Diluted Trypsin

Author:
Kuribara, Taiki,   et al.   ; Tauchi, Hanako; Imashiro, Chikahiro; Fujii, Genichiro; Kurashina, Yuta; Totani, Kiichiro; Takemura, Kenjiro;     Show all 7 Authors
Source:
Biotechnology and bioprocess engineering 2019 v.24 no.3 pp. 536-543
ISSN:
1226-8372
Subject:
adverse effects; bioengineering; carcinogenesis; cell culture; glucose; mechanical stress; membrane proteins; trypsin; ultrasonics; viability; vibration
Abstract:
... Bioengineering research and applications are supported by cell culture technologies that produce a large number of homogeneous cells. However, trypsin used in the general culture procedure for cell detachment decreases cell activity and culture efficiency. Furthermore, manually conducted culture procedures, especially pipetting after trypsin treatment, can induce inhomogeneous mechanical stress in ...
DOI:
10.1007/s12257-018-0491-2

18. Structural Insights into the Inhibition of Amyloid Fibril Formation by Fibrinogen via Interaction with Prefibrillar Intermediates

Author:
Akai, Taiki,   et al.   ; Yamamoto, Naoki; Inoue, Rintaro; Sugiyama, Masaaki; Tamura, Atsuo; Chatani, Eri;     Show all 6 Authors
Source:
Biochemistry 2019 v.58 no.24 pp. 2769-2781
ISSN:
1520-4995
Subject:
amyloid; fibrinogen; insulin; models; molecular chaperones; neurodegenerative diseases; protein secretion; small-angle X-ray scattering; stoichiometry
Abstract:
... Abnormal protein aggregation tends to result in the formation of β-sheet rich amyloid fibrils, which are related to various kinds of amyloidoses and neurodegenerative diseases. The susceptibility to aggregation of protein molecules is dealt with by proteostasis in living systems, in which molecular chaperones play an important role. Recently, several secreted proteins have been examined as extrace ...
DOI:
10.1021/acs.biochem.9b00439

19. Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy

Author:
Yanagishima, Taiki,   et al.   ; Liu, Yanyan; Curran, Arran; Edmond, Kazem V.; Sacanna, Stefano; Dullens, Roel P. A.;     Show all 6 Authors
Source:
Langmuir 2019 v.35 no.24 pp. 7962-7969
ISSN:
1520-5827
Subject:
confocal laser scanning microscopy; image analysis; models; optical traps; refractive index; solvents
Abstract:
... We describe the synthesis and application of 3-(trimethoxysilyl)propyl methacrylate (TPM) particles as a colloidal model system for three-dimensional (3D) confocal scanning laser microscopy. The effect of the initial TPM concentration on the growth and polydispersity of the particles and a recently developed solvent transfer method to disperse particles in a refractive index and density-matching s ...
DOI:
10.1021/acs.langmuir.9b00963

20. Anti-inflammatory marine cyclic peptide stylissatin A and its derivatives inhibit differentiation of murine preadipocytes

Author:
Sunaba, Taiki,   et al.   ; Zhang, Menghua; Sun, Yiting; Sasaki, Kazunori; Isoda, Hiroko; Kigoshi, Hideo; Kita, Masaki;     Show all 7 Authors
Source:
Chemical communications 2019 v.55 no.38 pp. 5471-5474
ISSN:
1364-548X
Subject:
adipocytes; anti-obesity agents; chemical reactions; cytotoxicity; mice; peptides; triacylglycerols
Abstract:
... Stylissatin A, an anti-inflammatory cyclic heptapeptide, and its derivatives potently inhibited the differentiation of preadipocytes and reduced triglyceride accumulation in mature adipocytes, with little cytotoxicity. Our studies might contribute to the development of leads for new anti-inflammatory and anti-obesity agents. ...
DOI:
10.1039/c9cc02517k