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You searched for: Publication Year 2018 Remove constraint Publication Year: 2018 Source 2018 v.154 Remove constraint Source: 2018 v.154 Subject gene expression Remove constraint Subject: gene expression Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Differentiation of sympatric zebra and quagga mussels in ecotoxicological studies: A comparison of morphometric data, gene expression, and body metal concentrations

Author:
Kerambrun, E.; Delahaut, L.; Geffard, A.; David, E.
Source:
Ecotoxicology and environmental safety 2018 v.154 pp. 321-328
ISSN:
0147-6513
Subject:
Dreissena bugensis; Dreissena polymorpha; H+/K+-exchanging ATPase; H-transporting ATP synthase; amylases; biochemical pathways; biomarkers; cadmium; catalase; cytochrome-c oxidase; ecotoxicology; freshwater molluscs; gene expression; genes; genetic markers; glutathione transferase; lead; morphometry; oxidative stress; rivers; sequence analysis; sympatry; toxic substances; France
Abstract:
... The zebra mussel is among the best studied freshwater molluscs in ecotoxicology, but information on the quagga mussel is lacking. Considering its potential spread, we selected a river in France in which zebra and quagga mussels coexisted, and then we used genetic markers to differentiate the two species and compared morphological parameters. cDNA sequencing assays of ten genes already used in zebr ...
DOI:
10.1016/j.ecoenv.2018.02.051

2. Response of PXR signaling pathway to simvastatin exposure in mosquitofish (Gambusia affinis) and its histological changes

Author:
Bao, Shuang; Nie, Xiangping; Liu, Yang; Wang, Chao; Liu, Sijia
Source:
Ecotoxicology and environmental safety 2018 v.154 pp. 228-236
ISSN:
0147-6513
Subject:
Gambusia affinis; P-glycoproteins; Western blotting; adverse effects; aquatic environment; enzyme activity; exposure duration; freshwater fish; gene expression; genes; histology; lipids; liver; messenger RNA; multiple drug resistance; nontarget organisms; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; tissues; transcription (genetics); transcription factors; transmission electron microscopes
Abstract:
... As a widely used lipid lowering agent, simvastatin recently has been frequently detected in aquatic environment and the potential adverse effects from simvastatin exposure to non-target organisms such as fish is worthy of more attention. The aim of this study was to reveal the responses of detoxification system in fish to simvastatin exposure. In this investigation a ubiquitous small freshwater fi ...
DOI:
10.1016/j.ecoenv.2018.02.015

3. A simple and highly efficient method for gene silencing in Escherichia coli

Author:
Magistro, Giuseppe; Magistro, Christiane; Stief, Christian G.; Schubert, Sören
Source:
Journal of microbiological methods 2018 v.154 pp. 25-32
ISSN:
0167-7012
Subject:
Escherichia coli; antisense RNA; bacteria; gene expression; gene silencing; genes; open reading frames; plasmids; protocols; translation (genetics)
Abstract:
... Here we present a simple and rapidly achievable protocol for gene silencing in Escherichia coli (E. coli). In this procedure, antisense RNA (asRNA) of 400-nucleotides (nt) length and absolute complementarity to the target is produced by an expression plasmid. The designed asRNA should ideally cover at least the −10 site of the promoter and the Shine-Dalgarno sequence, and additional 300-bp of the ...
DOI:
10.1016/j.mimet.2018.10.003

4. FOXD3 acts as a repressor of the mitochondrial S-adenosylmethionine carrier (SLC25A26) gene expression in cancer cells

Author:
Cianciulli, Antonia; Menga, Alessio; Ferdinando, Palmieri; Iacobazzi, Vito
Source:
Biochimie 2018 v.154 pp. 25-34
ISSN:
0300-9084
Subject:
S-adenosylmethionine; catalytic activity; cytosol; folic acid; gene expression; genes; methylation; mitochondria; neoplasm cells
Abstract:
... The mitochondrial S-adenosylmethionine carrier (SAMC), encoded by the SLC25A26 gene, catalyzes the uptake of S-adenosylmethionine (SAM) from the cytosol into mitochondria in exchange for S-adenosylhomocysteine (SAH), produced inside the mitochondria. In the last years we have been functionally characterizing the promoter of SLC25A26 gene. In this study we show that a silencer activity is present i ...
DOI:
10.1016/j.biochi.2018.07.025

5. Rapid and efficient protocol to introduce exogenous DNA in Vibrio harveyi and Pseudoalteromonas sp.

Author:
Delavat, François; Bidault, Adeline; Pichereau, Vianney; Paillard, Christine
Source:
Journal of microbiological methods 2018 v.154 pp. 1-5
ISSN:
0167-7012
Subject:
Pseudoalteromonas; Vibrio campbellii; Vibrio harveyi; bioluminescence; electroporation; gene expression; genes; phenotypic variation; plasmids
Abstract:
... Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudo ...
DOI:
10.1016/j.mimet.2018.09.022