Jump to Main Content
- Hao, Jingfang; Pétriacq, Pierre; de Bont, Linda; Hodges, Michael; Gakière, Bertrand
- Plant science 2018 v.271 pp. 133-142
- Arabidopsis thaliana; Escherichia coli; NAD (coenzyme); apoproteins; aspartic acid; biochemical pathways; complementary DNA; enzyme activity; flavoproteins; gene expression; homeostasis; models; pH; pyridines; temperature
- ... The flavoprotein l-aspartate oxidase (LASPO) is the first enzyme of the de novo biosynthetic pathway of NAD+ in plants. Although LASPO is considered pivotal to maintain NAD+ homeostasis, it has not been hitherto characterized in plants. Here, the cDNA encoding the LASPO from the model plant Arabidopsis thaliana (AtLASPO, At5g14760) has been cloned and expressed in Escherichia coli for subsequent e ...