Jump to Main Content
- Ahn, Jun Ki; Kim, Hyo Yong; Park, Ki Soo; Park, Hyun Gyu
- Analytical chemistry 2018 v.90 no.19 pp. 11340-11343
- adenosine diphosphate; adenosine triphosphate; blood serum; cattle; cost effectiveness; enzymatic reactions; glucose; glucose 6-phosphate; hexokinase; humans; models; phosphates; pyruvate kinase; tap water; urine; washing
- ... We developed a label-free and washing-free method for biomolecular detection using a personal glucose meter (PGM). ATP was selected as a model target, and cascade enzymatic reactions promoted by hexokinase and pyruvate kinase were adopted to link the amount of ATP to glucose that is detectable by a hand-held PGM. In principle, the presence of target ATP enables hexokinase to catalyze the conversio ...