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You searched for: Publication year rev 7982-2018 Remove constraint Publication year rev: 7982-2018 Subject term actin Remove constraint Subject term: actin
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1. 174 Effects of Melatonin on In Vitro Maturation of Bovine Oocytes and Gene Expression in Cumulus Cells: Preliminary Results

Author:
F. C. Castro; L. Schefer; K. L. Schwarz; H. Fernandes; R. C. Botigelli; D. M. Paschoal; C. L. V. Leal
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 226-227
ISSN:
1031-3613
Subject:
RNA; actin; analysis of variance; animal reproduction; anti-inflammatory activity; antioxidant activity; antioxidants; apoptosis; carbon dioxide; cattle; complementary DNA; embryogenesis; follicle-stimulating hormone; gene expression; genes; gentamicin; in vitro culture; luteinizing hormone; melatonin; oocytes; oxidative stress; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; reverse transcription; slaughterhouses; sodium bicarbonate; sodium pyruvate
Abstract:
... Melatonin mediates several processes in animal reproduction and has drawn attention for its potent antioxidant, anti-apoptotic, anti-inflammatory action and, more recently, for its benefits on oocyte maturation and embryo development in vitro. The aim of this study was to assess the effect of melatonin during the in vitro maturation (IVM) on nuclear maturation of bovine oocytes and gene expression ...
DOI:
10.1071/RDv30n1Ab173

2. 209 Imaging the Molecular and Cell Dynamics that Form the Early Mouse Embryo

Author:
N. Plachta
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 245
ISSN:
1031-3613
Subject:
DNA; actin; animal tissues; developmental stages; human fertility; image analysis; mice; microtubules; placenta; pseudopodia; transcription (genetics); transcription factors
Abstract:
... Our goal is to reveal how mammalian cells resolve their fate, shape, and position in the body in real time. Understanding how these decisions are made is critical to realise how embryos form and what problems compromise human fertility. Yet, their real-time control in vivo remains unknown. Because fixed specimens cannot capture cell dynamics, we established imaging technologies to study cells dire ...
DOI:
10.1071/RDv30n1Ab209

3. 24 Evaluation of Latrunculin A for the Activation of Hand-Made Cloning (HMC) Porcine Embryos

Author:
F. K. Castañeda; N. G. Canel; G. V. Landschoot; A. De Stéfano; R. J. Bevacqua; D. F. Salamone
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 151-152
ISSN:
1031-3613
Subject:
actin; adults; air; blastocyst; carbon dioxide; chromosome number; cycloheximide; cytochalasin B; enzymatic treatment; extrusion; fibroblasts; metaphase; microfilaments; oocytes; parthenogenesis; progeny; somatic cells; swine; ultraviolet radiation
Abstract:
... Somatic cell nuclear transfer (SCNT) is an important biotechnological tool. However, production rates of viable offspring remain low. One possible cause of this low efficiency is chromosomal losses during early activation process (Liu et al. 2015 Cell. Reprogram. 17, 463–471). The use of actin inhibitors that block second polar body extrusion during activation protocols might be a strategy to avoi ...
DOI:
10.1071/RDv30n1Ab23

4. 3D Cell-Based High-Content Screening (HCS) Using a Micropillar and Microwell Chip Platform

Author:
Sang-Yun Lee; Il Doh; Do-Hyun Nam; Dong Woo Lee
Source:
Analytical chemistry 2018 v.90 no.14 pp. 8354-8361
ISSN:
1520-6882
Subject:
Western blotting; actin; alginates; antibodies; cell culture; cell viability; cultured cells; drugs; microfilaments; neoplasms; phenotype; screening; staining
Abstract:
... A micropillar/microwell chip platform was applied to develop a 3D cell-based high-content screening (HCS) platform. Previously, 3D cell culture in the micropillar/microwell chip platform was only limited to cell viability measurements in a high-throughput manner. However, an HCS system could provide biological and phenotypic information which was very useful to understand complex biological functi ...
DOI:
10.1021/acs.analchem.7b05328

5. A Comparative Proteome Approach Reveals Metabolic Changes Associated with Flammulina velutipes Mycelia in Response to Cold and Light Stress

Author:
Jing-yu Liu; Ming-chang Chang; Jun-long Meng; Cui-ping Feng; Yu Wang
Source:
Journal of agricultural and food chemistry 2018 v.66 no.14 pp. 3716-3725
ISSN:
1520-5118
Subject:
Flammulina velutipes; actin; autolysis; biosynthesis; calcium signaling; energy; fruiting bodies; fungi; gamma-aminobutyric acid; lysine; microtubules; morphogenesis; mushroom growing; mycelium; oxidative stress; phenylalanine; protein synthesis; proteome; secretion; stress response; tyrosine
Abstract:
... In some industrial processes, cold and light stresses are recognized as two important environmental triggers for the transformation of mycelia into fruit-bodies via intermediate primordia in Flammulina velutipes cultivation. To gain insights into the mechanism of regulation of F. velutipes mycelia in response to cold and light stress, proteins expressed abundantly and characteristically at particu ...
DOI:
10.1021/acs.jafc.8b00383

6. A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits

Author:
Leonardo Almeida-Souza; Rene A.W. Frank; Javier García-Nafría; Adeline Colussi; Nushan Gunawardana; Christopher M. Johnson; Minmin Yu; Gillian Howard; Byron Andrews; Yvonne Vallis; Harvey T. McMahon
Source:
Cell 2018 v.174 no.2 pp. 325-337.e14
ISSN:
0092-8674
Subject:
actin; endocytosis; ligands; mammals; microfilaments; oligomerization; plasma membrane; polymerization; scaffolding proteins
Abstract:
... Multiple proteins act co-operatively in mammalian clathrin-mediated endocytosis (CME) to generate endocytic vesicles from the plasma membrane. The principles controlling the activation and organization of the actin cytoskeleton during mammalian CME are, however, not fully understood. Here, we show that the protein FCHSD2 is a major activator of actin polymerization during CME. FCHSD2 deletion lead ...
DOI:
10.1016/j.cell.2018.05.020
PubMed:
29887380
PubMed Central:
PMC6057269

7. A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission

Author:
Melissa J. Hoyer; Patrick J. Chitwood; Christopher C. Ebmeier; Jonathan F. Striepen; Robert Z. Qi; William M. Old; Gia K. Voeltz
Source:
Cell 2018 v.175 no.1 pp. 254-265.e14
ISSN:
0092-8674
Subject:
actin; biotin; buds; endoplasmic reticulum; endosomes; membrane proteins
Abstract:
... Endoplasmic reticulum (ER) membrane contact sites (MCSs) mark positions where endosomes undergo fission for cargo sorting. To define the role of ER at this unique MCS, we targeted a promiscuous biotin ligase to cargo-sorting domains on endosome buds. This strategy identified the ER membrane protein TMCC1, a member of a conserved protein family. TMCC1 concentrates at the ER-endosome MCSs that are s ...
DOI:
10.1016/j.cell.2018.08.030
PubMed:
30220460
PubMed Central:
PMC6195207

8. A Spatially Explicit Model Shows How Titin Stiffness Modulates Muscle Mechanics and Energetics

Author:
Joseph D Powers; C David Williams; Michael Regnier; Thomas L Daniel
Source:
Integrative and comparative biology 2018 v.58 no.2 pp. 186-193
ISSN:
1557-7023
Subject:
actin; adenosine triphosphate; mechanical properties; models; monitoring; muscle strength; muscles; sarcomeres; skeletal muscle; striated muscle
Abstract:
... In striated muscle, the giant protein titin spans the entire length of a half-sarcomere and extends from the backbone of the thick filament, reversibly attaches to the thin filaments, and anchors to the dense protein network of the z-disk capping the end of the half-sarcomere. However, little is known about the relationship between the basic mechanical properties of titin and muscle contractility. ...
DOI:
10.1093/icb/icy055
PubMed:
29897447
PubMed Central:
PMC6104699

9. A comparative analysis of Lophodermium fissuratum, sp. nov., found in haploxylon pine needles in the Pacific Northwest, and other Lophodermium endophytes

Author:
Rodolfo Salas-Lizana; Ryoko Oono
Source:
Mycologia 2018 v.110 no.5 pp. 797-810
ISSN:
1557-2536
Subject:
actin; ascomata; endophytes; fungi; genes; hosts; internal transcribed spacers; new species; phylogeny
Abstract:
... Lophodermium is a large fungal genus consisting of over 100 named species, with ca. 38 of these commonly found as endophytes of pine needles. In this study, we use both morphological and sequencing data to describe a new Lophodermium species associated with haploxylon pines from the Pacific Northwest. This new species resembled the morphology of L. nitens, another commonly occurring species from t ...
DOI:
10.1080/00275514.2018.1499991

10. A comparative study of the antitumor efficacy of peptide-doxorubicin conjugates with different linkers

Author:
Yanqin Liang; Suxin Li; Xueling Wang; Yuan Zhang; Yanan Sun; Yaoqi Wang; Xiaoyou Wang; Bing He; Wenbing Dai; Hua Zhang; Xueqing Wang; Qiang Zhang
Source:
Journal of controlled release 2018 v.275 pp. 129-141
ISSN:
0168-3659
Subject:
actin; biocompatibility; cathepsin B; cytotoxicity; doxorubicin; endocytosis; integrins; ligands; lysosomes; mice; models; physiological transport; synergism; therapeutics
Abstract:
... The peptide-drug conjugates caused much attention currently. The purpose of present study was to elucidate the possible synergistic effect between ligand peptide and stimuli-responsive linkage in amphiphilic peptide-drug conjugates (APDCs) with different linkers. Especially, the superiority of each strategy as well as the synergistic effect between them was carefully investigated via the parallel ...
DOI:
10.1016/j.jconrel.2018.01.033

11. A genome editing vector that enables easy selection and identification of knockout cells

Author:
Akira Nagasaki; Yoshio Kato; Keiichi Meguro; Ayana Yamagishi; Chikashi Nakamura; Taro Q.P. Uyeda
Source:
Plasmid 2018 v.98 pp. 37-44
ISSN:
0147-619X
Subject:
actin; blasticidin S; cell lines; enzymes; gene editing; genes; humans; mice; monitoring; plasmids
Abstract:
... The CRISPR/Cas9 system is a powerful genome editing tool for disrupting the expression of specific genes in a variety of cells. However, the genome editing procedure using currently available vectors is laborious, and there is room for improvement to obtain knockout cells more efficiently. Therefore, we constructed a novel vector for high efficiency genome editing, named pGedit, which contains EGF ...
DOI:
10.1016/j.plasmid.2018.08.005

12. A new avian Cryptosporidium genotype in a 1-month-old caged brown wood owl (Strix leptogrammica) with severe dehydration and diarrhea

Author:
Ikuko Makino; Mizue Inumaru; Niichiro Abe; Yukita Sato
Source:
Parasitology research 2018 v.117 no.9 pp. 3003-3008
ISSN:
0932-0113
Subject:
Cryptosporidium; DNA; Strix; actin; anorexia; birds; diarrhea; feces; genes; genotype; heat shock proteins; loci; microscopy; mixed infection; oocysts; parasites; polymerase chain reaction; vomiting; wood; Japan
Abstract:
... A 1-month-old brown wood owlet (Strix leptogrammica) purchased from a wholesaler and housed as a companion bird by an individual owner in Japan showed severe dehydration and anorexia following a week of vomiting and severe diarrhea. A great number of approximately 5 × 4-μm-sized Cryptosporidium oocysts were found in the feces by microscopy. The owlet was administered subcutaneous fluid and intraga ...
DOI:
10.1007/s00436-018-5951-2

13. A new toolset for protein expression and subcellular localization studies in citrus and its application to citrus tristeza virus proteins

Author:
Amit Levy; Choaa El-Mochtar; Chunxia Wang; Michael Goodin; Vladimir Orbovic
Source:
Plant methods 2018 v.14 no.1 pp. 2
ISSN:
1746-4811
Subject:
Agrobacterium; Arabidopsis; Citrus; Citrus tristeza virus; Xanthomonas citri; actin; biolistics; coat proteins; endoplasmic reticulum; fluorescent proteins; gene expression; genes; greening disease; leaves; protein synthesis; tissues; transgenic plants; trees
Abstract:
... BACKGROUND: Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene constructs, and there is a need for a more robust transient expression system in citrus leaves. Biolistic particle delivery is an alternative to Agrobacte ...
DOI:
10.1186/s13007-017-0270-7
PubMed:
29339969
PubMed Central:
PMC5759842

14. A pathogenetic role for M1 macrophages in peritoneal dialysis-associated fibrosis

Author:
Qing Li; Min Zheng; Yueheng Liu; Wei Sun; Jun Shi; Jie Ni; Qiong Wang
Source:
Molecular immunology 2018 v.94 pp. 131-139
ISSN:
0161-5890
Subject:
Toll-like receptor 4; actin; animal models; cadherins; dialysis; fibrin; fibronectins; fibrosis; histology; macrophages; mice; monocytes; muscles; phenotype; ultrafiltration
Abstract:
... Peritoneal fibrosis (PF) is a frequent complication of peritoneal dialysis (PD) accompanied by the infiltration of inflammatory cells. Recently, the function of macrophages in an inflammatory microenvironment during PD remains unknown. This study aimed to elucidate the role of distinct macrophage phenotypes in the progression of PF through macrophage depletion in a peritoneal dialysis-induced mous ...
DOI:
10.1016/j.molimm.2017.12.023

15. A peptidomic approach of meat protein degradation in a low-sodium fermented sausage model using autochthonous starter cultures

Author:
Marcio Aurélio de Almeida; Erick Saldaña; Jair Sebastião da Silva Pinto; Jorge Palacios; Carmen J. Contreras-Castillo; Miguel Angel Sentandreu; Silvina Graciela Fadda
Source:
Food research international 2018 v.109 pp. 368-379
ISSN:
0963-9969
Subject:
Enterococcus; Lactobacillus; actin; amino acids; fermented sausages; free amino acids; lactic acid bacteria; meat protein; models; molecular weight; myofibrillar proteins; off flavors; peptides; protein degradation; sodium chloride; starter cultures
Abstract:
... Fermented sausage technology is currently compromised in decreasing the addition of NaCl. Use of starter cultures with peptidogenic potential could be a valuable strategy that can mask or hide off flavors produced by the use of NaCl substituents. In the present work, the peptidogenic potential of four lactic acid bacteria species was evaluated in a low-sodium beaker sausage (BS) model. Using a pep ...
DOI:
10.1016/j.foodres.2018.04.042

16. A role for myosin VI in retinal pigment epithelium phagocytosis

Author:
Navindi Hewage; David Altman
Source:
Biochemical and biophysical research communications 2018 v.504 no.4 pp. 759-764
ISSN:
0006-291X
Subject:
actin; cell lines; epithelium; eyes; fluorescence; humans; microparticles; microtubules; myosin; phagocytosis; phagosomes; photoreceptors; wastes
Abstract:
... The retinal pigment epithelium (RPE) is a monolayer of pigmented cells adjacent to the choroid coat, the vascular layer of the eye. Among other functions, these cells are responsible for the phagocytosis of rod and cone cell waste shed from the photoreceptor outer segments. We describe here studies to understand the involvement of the motor protein myosin VI in the trafficking of internalized micr ...
DOI:
10.1016/j.bbrc.2018.09.006

17. A ternary-complex of a suicide gene, a RAGE-binding peptide, and polyethylenimine as a gene delivery system with anti-tumor and anti-angiogenic dual effects in glioblastoma

Author:
Eunji Choi; Jungju Oh; Dahee Lee; Jaewon Lee; Xiaonan Tan; Minkyung Kim; Gyeungyun Kim; Chunxian Piao; Minhyung Lee
Source:
Journal of controlled release 2018 v.279 pp. 40-52
ISSN:
0168-3659
Subject:
DNA; actin; advanced glycation end products; angiogenesis; animal models; antagonists; antineoplastic activity; apoptosis; endothelial cells; glioblastoma; macrophages; muscles; polyethyleneimine; secretion; suicide genes; therapeutics; transfection; vascular endothelial growth factors
Abstract:
... The receptor for advanced glycation end-products (RAGE) is involved in tumor angiogenesis. Inhibition of RAGE might be an effective anti-angiogenic therapy for cancer. In this study, a cationic RAGE-binding peptide (RBP) was produced as an antagonist of RAGE, and a ternary-complex consisting of RBP, polyethylenimine (2 kDa, PEI2k), and a suicide gene (pHSVtk) was developed as a gene delivery syste ...
DOI:
10.1016/j.jconrel.2018.04.021

18. A toolkit for studying cellular reorganization during early embryogenesis in Arabidopsis thaliana

Author:
Che‐Yang Liao; Dolf Weijers
Source:
plant journal 2018 v.93 no.6 pp. 963-976
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; actin; cell division; cell polarity; digital images; embryogenesis; fluorescence; genetic factors; microtubules; models; morphogenesis; ontogeny; tissues; topology
Abstract:
... Considerable progress has been made in understanding the influence of physical and genetic factors on the patterns of cell division in various model systems. However, how each of these factors directs changes in subcellular structures has remained unclear. Generic machineries for the execution of cell expansion and division have been characterized, but how these are influenced by genetic regulator ...
DOI:
10.1111/tpj.13841
PubMed:
29383853
PubMed Central:
PMC5887935

19. Abiotic stress and tissue-specific reference genes for quantitative reverse transcription PCR analysis in Korean native watermelons, Citrullus lanatus ‘Black-King’ and ‘Speed-Plus-Honey’

Author:
Soo Jin Kim; Il Lae Jung; Hye-Eun Lee; Ji-Hoon Lee
Source:
Han'guk Ŭngyong Saengmyŏng Hwahakhoe chi = 2018 v.61 no.5 pp. 593-598
ISSN:
1738-2203
Subject:
Arabidopsis; Citrullus lanatus; abiotic stress; abscisic acid; actin; cucumbers; cultivars; databases; drought; flowers; gene expression; genes; glyceraldehyde-3-phosphate dehydrogenase; leaves; nucleotide sequences; peptide elongation factors; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ribosomal RNA; salt concentration; screening; seedlings; sodium chloride; ubiquitin-protein ligase; watermelons
Abstract:
... A wide variety of research on watermelon has been conducted, and such studies have been motivated by the published genome sequence database of watermelon. Screening of proper reference genes is the primary step for normalization in gene expression analysis. Based on previous studies conducted on Arabidopsis and cucumber, we selected eight candidate reference genes of ClACT, ClEF1α, ClGAPDH, ClIDH, ...
DOI:
10.1007/s13765-018-0391-3

20. Actin Polymerization Mediated by AtFH5 Directs the Polarity Establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis

Author:
Chang Liu; Yi Zhang; Haiyun Ren
Source:
Molecular plant 2018 v.11 no.11 pp. 1389-1399
ISSN:
1674-2052
Subject:
Arabidopsis; actin; microfilaments; physiological transport; plant reproduction; plasma membrane; pollen; pollen germination; polymerization
Abstract:
... The process of pollen germination is crucial for flowering plant reproduction, but the mechanisms through which pollen grains establish polarity and select germination sites are not well understood. In this study, we report that a formin family protein, AtFH5, is localized to the vesicles and rotates ahead of Lifeact-mEGFP-labeled actin filaments during pollen germination. The translocation of AtF ...
DOI:
10.1016/j.molp.2018.09.004

21. Actin as a cytoskeletal basis for cell architecture and a protein essential for ecdysis in Prorocentrum minimum (Dinophyceae, Prorocentrales)

Author:
Mariia Berdieva; Ilya Pozdnyakov; Olga Matantseva; Nikolay Knyazev; Sergei Skarlato
Source:
Phycological research 2018 v.66 no.2 pp. 127-136
ISSN:
1322-0829
Subject:
Prorocentrum; actin; cell nucleolus; chromosomes; deoxyribonuclease I; ecdysis; electron microscopy; fluorescence; fluorescent labeling; isothiocyanates; microfilaments; microtubules; staining
Abstract:
... The specific cell architecture of prorocentroid dinoflagellates is reflected in the internal cell structure, particularly, in cytoskeleton organization. Cytoskeleton arrangement in a Prorocentrum minimum cell was investigated using fluorescent labeling approaches, electron‐microscopy and immunocytochemical methods. The absence of cortical microtubules was confirmed. Phalloidin – tetramethylrhodami ...
DOI:
10.1111/pre.12214

22. Actin cytoskeleton and sperm function

Author:
Haim Breitbart; Maya Finkelstein
Source:
Biochemical and biophysical research communications 2018 v.506 no.2 pp. 372-377
ISSN:
0006-291X
Subject:
actin; eggs; exocytosis; female reproductive system; mammals; microfilaments; oocytes; polymerization; sperm capacitation; spermatozoa
Abstract:
... For the acquisition of the ability to fertilize the egg, mammalian spermatozoa should undergo a series of biochemical transformations in the female reproductive tract, collectively called capacitation. The capacitated sperm can undergo the acrosomal exocytosis process near or on the oocyte, which allows the spermatozoon to penetrate and fertilize it. One of the main processes in capacitation invol ...
DOI:
10.1016/j.bbrc.2017.11.001

23. Actin filaments regulate the adhesion between the plasma membrane and the cell wall of tobacco guard cells

Author:
Qin Yu; Jing-Jing Ren; Lan-Jing Kong; Xiu-Ling Wang
Source:
Protoplasma 2018 v.255 no.1 pp. 235-245
ISSN:
0033-183X
Subject:
actin; cell walls; depolymerization; endocytosis; guard cells; microfilaments; plasma membrane; plasmolysis; stomata; stomatal movement; tobacco
Abstract:
... During the opening and closing of stomata, guard cells undergo rapid and reversible changes in their volume and shape, which affects the adhesion of the plasma membrane (PM) to the cell wall (CW). The dynamics of actin filaments in guard cells are involved in stomatal movement by regulating structural changes and intracellular signaling. However, it is unclear whether actin dynamics regulate the a ...
DOI:
10.1007/s00709-017-1149-1

24. Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei

Author:
Xiaoxi Zhang; Xiaojun Zhang; Jianbo Yuan; Jiangli Du; Fuhua Li; Jianhai Xiang
Source:
Molecular genetics and genomics 2018 v.293 no.2 pp. 479-493
ISSN:
1617-4615
Subject:
Litopenaeus vannamei; Vibrio; White spot syndrome virus; actin; complementary DNA; disease control; hemocytes; multigene family; muscles; phylogeny; postlarvae; sequence analysis; shrimp; skeletal muscle; unigenes
Abstract:
... Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs ...
DOI:
10.1007/s00438-017-1397-y

25. Actin–myosin XI: an intracellular control network in plants

Author:
Zhongrui Duan; Motoki Tominaga
Source:
Biochemical and biophysical research communications 2018 v.506 no.2 pp. 403-408
ISSN:
0006-291X
Subject:
Arabidopsis thaliana; actin; cell growth; cytoplasmic streaming; eukaryotic cells; microfilaments; myosin; organelles; plant development
Abstract:
... Actin is one of the three major cytoskeletal components in eukaryotic cells. Myosin XI is an actin-based motor protein in plant cells. Organelles are attached to myosin XI and translocated along the actin filaments. This dynamic actin–myosin XI system plays a major role in subcellular organelle transport and cytoplasmic streaming. Previous studies have revealed that myosin-driven transport and the ...
DOI:
10.1016/j.bbrc.2017.12.169

26. Activation of human aortic valve interstitial cells by local stiffness involves YAP-dependent transcriptional signaling

Author:
Rosaria Santoro; Denis Scaini; Luisa Ulloa Severino; Francesco Amadeo; Silvia Ferrari; Giacomo Bernava; Gloria Garoffolo; Marco Agrifoglio; Loredana Casalis; Maurizio Pesce
Source:
Biomaterials 2018 v.181 pp. 268-279
ISSN:
0142-9612
Subject:
actin; atomic force microscopy; compliance; cytoskeleton; gels; humans; inflammation; patients; transcription (genetics); transcription factors
Abstract:
... Differentiation of valve interstitial cells (VICs) into pro-calcific cells is one of the central events in calcific aortic valve (AoV) disease (CAVD). While the paracrine pathways and the responsivity of VICs to mechanical compliance of the surrounding environment are well characterized, the molecular programming related to variations in local stiffness, and its link to cytoskeleton dynamics, is l ...
DOI:
10.1016/j.biomaterials.2018.07.033

27. Activation of β- and α2-adrenergic receptors stimulate tubulin polymerization and promote the association of Gβγ with microtubules in cultured NIH3T3 cells

Author:
Jorge A. Sierra-Fonseca; Christina Bracamontes; Jessica Saldecke; Siddhartha Das; Sukla Roychowdhury
Source:
Biochemical and biophysical research communications 2018 v.503 no.1 pp. 102-108
ISSN:
0006-291X
Subject:
actin; agonists; alpha-2 adrenergic receptors; cell division; cell movement; cyclic AMP; isoproterenols; microtubules; neurons; physiological transport; polymerization; signal transduction; tubulin; United Kingdom
Abstract:
... Microtubules (MTs) constitute a crucial part of the cytoskeleton and are essential for cell division and differentiation, cell motility, intracellular transport, and cell morphology. Precise regulation of MT assembly and dynamics is essential for the performance of these functions. Although much progress has been made in identifying and characterizing the cellular factors that regulate MT assembly ...
DOI:
10.1016/j.bbrc.2018.05.188
PubMed:
29852176
PubMed Central:
PMC6070407

28. Active Components from Sea Buckthorn (Hippophae rhamnoides L.) Regulate Hepatic Stellate Cell Activation and Liver Fibrogenesis

Author:
Guokun Zhang; Yifei Liu; Ping Liu
Source:
Journal of agricultural and food chemistry 2018 v.66 no.46 pp. 12257-12264
ISSN:
1520-5118
Subject:
DNA damage; DNA repair; Hippophae rhamnoides; actin; apoptosis; bile ducts; dose response; fibrosis; gene expression regulation; genes; inflammation; liver; liver function; muscles; nutritive value; rats; signal transduction; small fruits; transforming growth factor beta
Abstract:
... Sea buckthorn (Hippophae rhamnoides L.) is a berry bearing multiple nutritional properties. In this study, 46 compounds were isolated from sea buckthorn berries. Preliminary data showed that the components, C13, C15, and C32, exhibited profound inhibitory effect on the activation of hepatic stellate cells (HSCs) induced by transforming growth factor-β (TGF-β) and decreased the levels of inflammato ...
DOI:
10.1021/acs.jafc.8b05306

29. Addition of urea and thiourea to electrophoresis sample buffer improves efficiency of protein extraction from TCA/acetone‐treated smooth muscle tissues for phos‐tag SDS‐PAGE

Author:
Kosuke Takeya; Toshiyuki Kaneko; Motoi Miyazu; Akira Takai
Source:
Electrophoresis 2018 v.39 no.2 pp. 326-333
ISSN:
0173-0835
Subject:
acetone; actin; detection limit; lithium; muscles; myosin; phosphorylation; polyacrylamide gel electrophoresis; protein content; smooth muscle; sulfates; thiourea; trichloroacetic acid; urea
Abstract:
... Phosphorylation analysis by using phos‐tag technique has been reported to be suitable for highly sensitive quantification of smooth muscle myosin regulatory light chain (LC₂₀) phosphorylation. However, there is another factor that will affect the sensitivity of phosphorylation analysis, that is, protein extraction. Here, we optimized the conditions for total protein extraction out of trichloroacet ...
DOI:
10.1002/elps.201700394

30. Adiponectin inhibits osteoclastogenesis by suppressing NF-κB and p38 signaling pathways

Author:
Guiping Chen; Leitao Huang; Xia Wu; Xuqiang Liu; Qiang Xu; Fan Li; Min Dai; Bin Zhang
Source:
Biochemical and biophysical research communications 2018 v.503 no.3 pp. 2075-2082
ISSN:
0006-291X
Subject:
actin; adiponectin; bone density; ligands; osteoclasts; osteoporosis; resorption; signal transduction; transcription factor NF-kappa B
Abstract:
... Adiponectin (APN) has been shown to play a key role in regulating bone mineral density (BMD). Nevertheless, the effects of APN on receptor activator of NF-κB ligand (RANKL)-induced osteoclast formation and mechanism of regulation are not entirely clear. The study, therefore, aimed to evaluate the effect of APN on osteoclastogenesis. Our results showed that APN inhibits osteoclastogenesis and resor ...
DOI:
10.1016/j.bbrc.2018.07.162

31. Agrobacterium-mediated gene delivery and transient expression in the red macroalga Chondrus crispus

Author:
Anusha Devi Ramessur; John H. Bothwell; Christine A. Maggs; Sook Yee Gan; Siew Moi Phang
Source:
Botanica marina 2018 v.61 no.5 pp. 499-510
ISSN:
1437-4323
Subject:
Chlorophyta; Chondrus crispus; acetosyringone; actin; bacteria; beta-glucuronidase; coculture; gene transfer; genetically modified organisms; macroalgae; reporter genes; scanning electron microscopy; staining
Abstract:
... Molecular resources and transgenic studies in red algae are lagging behind those for green algae. The Agrobacterium-mediated gene-transfer method routinely used in plant transformation has not been fully utilised in the red algae, which, as an important source of phycocolloids, warrant more studies. In this regard, a stepwise methodology was developed for Agrobacterium-mediated transformation of t ...
DOI:
10.1515/bot-2018-0028

32. Alogliptin abates memory injuries of hepatic encephalopathy induced by acute paracetamol intoxication via switching-off autophagy-related apoptosis

Author:
Muhammed A. Saad; Alyasaa A. Rastanawi; Muhammed F. El-Yamany
Source:
Life sciences 2018 v.215 pp. 11-21
ISSN:
0024-3205
Subject:
acetaminophen; acetylcholine; acetylcholinesterase; actin; alanine transaminase; alkaline phosphatase; ammonia; apoptosis; aspartate transaminase; autophagy; bioavailability; blood; caspase-3; cytochrome c; dopamine; encephalopathy; enzyme activity; gamma-aminobutyric acid; glutamic acid; hepatocytes; hepatotoxicity; hippocampus; histopathology; inflammation; ligands; liver; memory; memory disorders; muscles; neurotransmitters; norepinephrine; poisoning; rats; serotonin; transforming growth factor beta 1; tumor necrosis factor-alpha
Abstract:
... Hepatic encephalopathy (HE) is a complex neuropsychiatric syndrome. Paracetamol (APAP) causes, in high doses, a hepatic injury. Alogliptin (ALO), with its 100% oral bioavailability, may be able to reverse the acute hepatic injury and memory impairments.Forty rats were divided into four groups as follows; Normal Control Group, APAP intoxicated group, ALO and SIL groups. Behavioral tests (Morris wat ...
DOI:
10.1016/j.lfs.2018.10.069

33. Alterations in the distribution of actin and its binding proteins in the porcine endometrium during early pregnancy: Possible role in epithelial remodeling and embryo adhesion

Author:
Beenu Moza Jalali; Pawel Likszo; Aneta Andronowska; Dariusz J. Skarzynski
Source:
Theriogenology 2018 v.116 pp. 17-27
ISSN:
0093-691X
Subject:
actin; adhesion; binding proteins; conceptus; crosslinking; cytoskeleton; endometrium; epithelial cells; epithelium; estrous cycle; gene expression; integrins; non-specific protein-tyrosine kinase; polymerization; pregnancy; staining; swine; trophoblast
Abstract:
... During early pregnancy, uterine epithelial cells undergo major transformations in their cytoskeleton that make the endometrium receptive for conceptus attachment. Actin binding proteins (ABPs) such as cofilin, gelsolin, and vinculin are involved in regulating actin polymerization, severing or crosslinking actin to integrins. However, whether ABPs are involved in epithelial remodeling or embryo adh ...
DOI:
10.1016/j.theriogenology.2018.05.004

34. Altering equine corneal fibroblast differentiation through Smad gene transfer

Author:
Todd L. Marlo; Elizabeth A. Giuliano; Ratnakar Tripathi; Ajay Sharma; Rajiv R. Mohan
Source:
Veterinary ophthalmology 2018 v.21 no.2 pp. 132-139
ISSN:
1463-5216
Subject:
actin; clones; cornea; fibroblasts; fibrosis; fluorescent antibody technique; gene overexpression; gene therapy; gene transfer; genes; horses; ophthalmology; plasmids; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; smooth muscle; transforming growth factor beta 1
Abstract:
... OBJECTIVE: To explore the impact of equine corneal fibroblast (ECF) to myofibroblast (ECM) differentiation by altering the expression of the Smad genes either individually or in combination. Specifically, we sought to examine the ECF differentiation after (a) silencing of Smad2, 3, and 4 profibrotic genes individually and (b) overexpression of antifibrotic Smad7 gene and in a combination with pro‐ ...
DOI:
10.1111/vop.12485
PubMed:
28685927
PubMed Central:
PMC5756533

35. An actin-based nucleoskeleton involved in gene regulation and genome organization

Author:
Xin Xie; Piergiorgio Percipalle
Source:
Biochemical and biophysical research communications 2018 v.506 no.2 pp. 378-386
ISSN:
0006-291X
Subject:
DNA; actin; chromatin; environmental factors; epigenetics; eukaryotic cells; gene activation; genes; mammals
Abstract:
... In eukaryotic cells gene regulation is dependent on global genome organization. This is achieved, in response to favorable environmental conditions, through spatial redistribution of chromatin and changes in global epigenetic levels. This eventually drives movement of gene-rich chromatin loops and formation of DNA loops, consolidating neighborhoods of gene expression and silencing. One of the chal ...
DOI:
10.1016/j.bbrc.2017.11.206

36. An ex vivo abomasal ovine model to study the immediate immune response in the context of Haemonchus contortus larval-stage

Author:
Saeed El-Ashram; Cuiping Li; Fathi Abouhajer; Rashid Mehmood; Ibrahim Al Nasr; Yinghui Zhang; Tang Lu; Ding Yili; Xun Suo; Zhang Haoji; Zhili Li; Shujian Huang
Source:
Veterinary parasitology 2018 v.254 pp. 105-113
ISSN:
0304-4017
Subject:
Haemonchus contortus; actin; animal models; animal tissues; atypical chemokine receptor 3; explants; galectins; gene expression regulation; genes; histology; immune response; integrins; intercellular adhesion molecule-1; interleukin-6; interleukin-8; lactate dehydrogenase; nematode infections; sequence analysis; sheep; superoxide dismutase; transcription (genetics)
Abstract:
... We have set up an ex vivo ovine abomasal model, which can mimic the multicellular process to explore the early steps in haemonchine nematode infection using RNA-seq technology. Ovine abomasal explants were collected for histological and transcriptional analysis and supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. Atotal of 233 were substantially induced genes between L4-in ...
DOI:
10.1016/j.vetpar.2018.02.042

37. An optogenetic approach to control protein localization during embryogenesis of the sea urchin

Author:
Alicia Uchida; Mamiko Yajima
Source:
Developmental biology 2018 v.441 no.1 pp. 19-30
ISSN:
0012-1606
Subject:
Avena sativa; Echinoidea; actin; blue light; cell cycle; cytotoxicity; gastrulation; germ cells; irradiation; microtubules; optogenetics; peptides; photosensitivity; protein-protein interactions
Abstract:
... Light inducible protein–protein interactions have been used to manipulate protein localization and function in the cell with utmost spatial and temporal precision. In this technical report, we use a recently developed optogenetic approach to manipulate protein localization in the developing sea urchin embryo. A photosensitive LOV domain from Avena sativa phototropin1 cages a small peptide that bin ...
DOI:
10.1016/j.ydbio.2018.06.015
PubMed:
29958898
PubMed Central:
PMC6089079

38. Analysis and identification of tyrosine phosphorylated proteins in hemocytes of Litopenaeus vannamei infected with WSSV

Author:
Xiaoqian Tang; Xiaoai Li; Fude Zhai; Jing Xing; Xiuzhen Sheng; Wenbin Zhan
Source:
Fish & shellfish immunology 2018 v.82 pp. 84-91
ISSN:
1050-4648
Subject:
H+/K+-exchanging ATPase; H-transporting ATP synthase; Litopenaeus vannamei; Western blotting; White spot syndrome virus; actin; adenosinetriphosphatase; enzyme-linked immunosorbent assay; fish; flow cytometry; fluorescence; fluorescent antibody technique; gene expression regulation; hemocytes; immune response; mass spectrometry; phosphopyruvate hydratase; phosphorylation; protein disulfide-isomerase; protein kinases; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; shellfish; shrimp; two-dimensional gel electrophoresis; tyrosine; ubiquitin
Abstract:
... Previous studies have demonstrated that protein tyrosine phosphorylation plays an important role in WSSV infection. In the present work, in order to further elucidate the potential role of protein tyrosine phosphorylation in white spot syndrome virus (WSSV) infection. The expression variation of tyrosine phosphorylated proteins in hemocytes of shrimp (Litopenaeus vannamei) after WSSV infection wer ...
DOI:
10.1016/j.fsi.2018.08.017

39. Analysis of differentially expressed proteins in the pollen of Malus spectabilis (crabapples) and Malus domestica (‘Gala’ apples) and their role in pollination

Author:
Rong Wang; Xiang Shen; Wenhui Zhang
Source:
Journal of horticultural science & biotechnology 2018 v.93 no.6 pp. 566-572
ISSN:
2380-4084
Subject:
Malus domestica; actin; apples; beta-fructofuranosidase; chloroplasts; cytochrome b; databases; ferritin; phosphoglucomutase; pollen; pollination; protein subunits; protein synthesis; proton pump; regulatory proteins; signal transduction; tandem mass spectrometry; two-dimensional gel electrophoresis
Abstract:
... Our objective was to study the differentially expressed proteins (DEP) in various Malus spectabilis (crabapple) varieties (M. ‘Snowdrift’, M. ‘Hongling’ and M. ‘Hongjin’) in relation to Malus domestica (‘Gala’) and their role in pollination. Our method used a two-dimensional electrophoresis (2-DE) to analyse the differential proteins in the pollen of several crabapples. The 2-DE apples combined wi ...
DOI:
10.1080/14620316.2018.1433965

40. Analysis of virulence and genetic variability of Alternaria alternata associated with leaf spot disease in potato plants in Iran

Author:
Mehdi Nasr Esfahani
Source:
Acta mycologica 2018 v.53 no.1 pp. am.1105
ISSN:
2353-074X
Subject:
Alternaria alternata; actin; adenosinetriphosphatase; allergens; calmodulin; data collection; fruits; genes; genetic variation; glyceraldehyde-3-phosphate dehydrogenase; leaf spot; microsatellite repeats; pathogens; phylogeny; plasma membrane; postharvest storage; potatoes; provenance; random amplified polymorphic DNA technique; sequence analysis; virulence; Iran
Abstract:
... Leaf spot disease in potato is caused by Alternaria alternata (Fr.) Keissler, an opportunistic pathogen that infests many agricultural crops worldwide in the field and during postharvest storage of vegetables and fruits. Alternaria alternata is associated with leaf spot disease in potato in Iran. Thus, there is a need to investigate the virulence and genetic variability of Iranian A. alternata iso ...
DOI:
10.5586/am.1105

41. Angiotensin-I-Converting Enzyme Inhibitory Activity and Antioxidant Properties of Cryptides Derived from Natural Actomyosin of Catla catla Using Papain

Author:
Krishnamoorthy Elavarasan; Bangalore Aswathnarayan Shamasundar
Source:
Journal of food quality 2018 v.2018 pp. -
ISSN:
0146-9428
Subject:
2,2-diphenyl-1-picrylhydrazyl; Catla catla; actin; antioxidant activity; enzyme inhibition; freshwater fish; hydrolysis; linoleic acid; myosin; papain; peroxidation
Abstract:
... Natural actomyosin (NAM) from the freshwater fish Catla catla was extracted and hydrolyzed using papain enzyme at different enzyme-to-substrate ratios (0.5%, 1.0%, 2.0%, 5.0%, and 10%) to obtain the cryptides with different degrees of hydrolysis (DH). Derived cryptides were evaluated for bioactive properties such as angiotensin-I-converting enzyme (ACE) inhibitory activity and antioxidant properti ...
DOI:
10.1155/2018/9354829

42. Anisotropic traction stresses and focal adhesion polarization mediates topography-induced cell elongation

Author:
Ai Kia Yip; Anh Tuan Nguyen; Muhammad Rizwan; Sum Thai Wong; Keng-Hwee Chiam; Evelyn K.F. Yim
Source:
Biomaterials 2018 v.181 pp. 103-112
ISSN:
0142-9612
Subject:
actin; anisotropy; cell growth; fibroblasts; focal adhesions; gels; polyacrylamide; polyethylene terephthalates
Abstract:
... Cell elongation and differentiation has been shown to be modulated by topographical cues provided by grating substratum. However, little is known about the mechanisms and forces involved in the grating-induced cell elongation, due to the difficulty in fabricating soft elastic gels that allow 3-dimensional (3D) cell traction stress measurements. In this paper, we present a method to fabricate soft ...
DOI:
10.1016/j.biomaterials.2018.07.057

43. Anti-fibrotic activity of polyphenol-enriched sugarcane extract in rats via inhibition of p38 and JNK phosphorylation

Author:
Liwen Wang; Min-Hsiung Pan; Chih-Yu Lo; Hui Zhao; Shiming Li; Chi-Tang Ho; Guliang Yang
Source:
Food & function 2018 v.9 no.2 pp. 951-958
ISSN:
2042-650X
Subject:
Saccharum officinarum; actin; alanine transaminase; blood serum; carbon; extracellular matrix proteins; fibrosis; high performance liquid chromatography; liver; liver cirrhosis; liver failure; mitogen-activated protein kinase; muscles; phosphorylation; polyphenols; portal hypertension; protective effect; rats; signal transduction; sugarcane; sugars; tissues; transforming growth factor beta 1
Abstract:
... Sugarcane (Saccharum officinarum L.), which is one of the most important sources of sugar, is also rich in polyphenolic compounds. In this study, polyphenols from sugarcane were extracted, and the dominant component was characterized quantitatively via HPLC to be (−)-epicatechin. Fibrosis occurs in many organs and is associated with severe tissue damage. Liver fibrosis is the excessive accumulatio ...
DOI:
10.1039/c7fo01617d

44. Apo-10’-lycopenoic acid inhibits cancer cell migration and angiogenesis and induces peroxisome proliferator-activated receptor γ

Author:
Junrui Cheng; Benchun Miao; Kang-Quan Hu; Xueyan Fu; Xiang-Dong Wang
Source:
Journal of nutritional biochemistry 2018 v.56 pp. 26-34
ISSN:
0955-2863
Subject:
actin; angiogenesis; animal models; cell movement; dose response; gelatinase A; gene expression; humans; liver; lung neoplasms; lungs; lycopene; messenger RNA; metastasis; neoplasm cells; peroxisome proliferator-activated receptors; pseudopodia
Abstract:
... We have previously shown that apo-10’-lycopenoic acid (ALA), a derivative of lycopene through cleavage by carotene-9’,10’-oxygenase, inhibits tumor progression and metastasis in both liver and lung cancer animal models. The underlying mechanism remains unknown. We hypothesized that ALA inhibits cancer cell motility and angiogenesis by up-regulating peroxisome proliferator-activated receptor γ (PPA ...
DOI:
10.1016/j.jnutbio.2018.01.003

45. Apple S‐RNase interacts with an actin‐binding protein, MdMVG, to reduce pollen tube growth by inhibiting its actin‐severing activity at the early stage of self‐pollination induction

Author:
Qing Yang; Dong Meng; Zhaoyu Gu; Wei Li; Qiuju Chen; Yang Li; Hui Yuan; Jie Yu; Chunsheng Liu; Tianzhong Li
Source:
plant journal 2018 v.95 no.1 pp. 41-56
ISSN:
0960-7412
Subject:
Malus domestica; actin; amino acids; apples; fluorescence; fluorescence microscopy; fluorescent antibody technique; microfilaments; mutagenesis; mutants; myosin; pollen tubes; ribonucleases; self-pollination
Abstract:
... In S‐RNase‐mediated self‐incompatibility, S‐RNase secreted from the style destroys the actin cytoskeleton of the self‐pollen tubes, eventually halting their growth, but the mechanism of this process remains unclear. In vitro biochemical assays revealed that S‐RNase does not bind or sever filamentous actin (F‐actin). In apple (Malus domestica), we identified an actin‐binding protein containing myos ...
DOI:
10.1111/tpj.13929

46. Application of uMelt SM algorithm in Real-time PCR melting curve analysis

Author:
Anahita Kharabi; Abbas Saedi
Source:
Biyutiknuluzhī-i kishāvarzī 2018 v.10 no.2 pp. 43-58
ISSN:
2228-6500
Subject:
DNA; Internet; Magnaporthe oryzae; actin; algorithms; compliance; computer software; denaturation; models; quantitative polymerase chain reaction; superoxide dismutase
Abstract:
... One of the challenges in interpreting the results obtained in qPCR is to make sure the amplicons are specific and that the melting curve analysis is used to examine it. Additional peaks in the melting curve is not always indicative of a problem, for this purpose, in this paper, a web-based tool called uMelt SM is suggested to researchers as a practical and simple way for the correct ana ...
DOI:
10.22103/jab.2018.2150

47. Arabidopsis FIM4 and FIM5 regulates the growth of root hairs in an auxin-insensitive way

Author:
X. Ding; S. Zhang; J. Liu; S. Liu; H. Su
Source:
Plant signaling & behavior 2018 v.13 no.9 pp. e1473667
ISSN:
1559-2324
Subject:
Arabidopsis; actin; cell growth; genetic lines; indole acetic acid; models; mutants; pollen tubes; root hairs
Abstract:
... Tip-growing cells provide a useful model system for studying the underlying mechanisms of plant cell growth. The apical growth of root hairs is dependent on the microfilament skeleton, and auxin is an important regulator of root hair development. We functionally characterized actin bundling proteins AtFIM4 and AtFIM5, which were preferentially expressed in tip-growing cells such as pollen tubes an ...
DOI:
10.1080/15592324.2018.1473667
PubMed:
30148414
PubMed Central:
PMC6204792

48. Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought

Author:
R. M. Acevedo; E. H. Avico; O. A. Ruiz; P. A. Sansberro
Source:
Biologia plantarum 2018 v.62 no.1 pp. 89-96
ISSN:
0006-3134
Subject:
DNA-directed RNA polymerase; Ilex paraguariensis; RNA; actin; computer software; cyclophilins; drought; gene expression; genes; leaves; quantitative polymerase chain reaction; reverse transcription; trees; tubulin; vacuoles; water stress
Abstract:
... Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, it requires a normalization using internal reference genes with stable expression. To address this issue, nine candidate reference genes were investigated in Ilex paraguarien ...
DOI:
10.1007/s10535-017-0754-3

49. Association between biomechanical alterations and migratory ability of semaphorin-3A-treated thymocytes

Author:
M.P. Lins; E.C.O. Silva; G.R. Silva; S.T. Souza; N.C. Medeiros; E.J.S. Fonseca; S. Smaniotto
Source:
Biochimica et biophysica acta 2018 v.1862 pp. 816-824
ISSN:
0304-4165
Subject:
actin; atomic force microscopy; biomechanics; bovine serum albumin; cell adhesion; cell membranes; cell movement; cell viability; cytoskeleton; flow cytometry; fluorescence microscopy; macrophages; mice; monocytes; neurons; pseudopodia; receptors; roughness; scanning electron microscopy; thymocytes
Abstract:
... Class 3 semaphorins are soluble proteins involved in cell adhesion and migration. Semaphorin-3A (Sema3A) was initially shown to be involved in neuronal guidance, and it has also been reported to be associated with immune disorders. Both Sema3A and its receptors are expressed by most immune cells, including monocytes, macrophages, and lymphocytes, and these proteins regulate cell function. Here, we ...
DOI:
10.1016/j.bbagen.2018.01.001

50. Astragaloside IV inhibits Angiotensin II-stimulated proliferation of rat vascular smooth muscle cells via the regulation of CDK2 activity

Author:
Deng-qing Zhang; Jin-song Li; Yu-mei Zhang; Feng Gao; Ruo-zhu Dai
Source:
Life sciences 2018 v.200 pp. 105-109
ISSN:
0024-3205
Subject:
Oriental traditional medicine; Western blotting; actin; adults; angiotensin II; astragalosides; atherosclerosis; cell cycle; cyclin-dependent kinase; enzyme activity; flow cytometry; muscles; myocytes; phenotype; rats; reverse transcriptase polymerase chain reaction; smooth muscle
Abstract:
... Astragaloside IV (AS-IV) is the central active component extracted from Radix astragali, an herbal remedy widely used in traditional Chinese medicine for the treatment of cardiovascular diseases. Aberrant proliferation of vascular smooth muscle cells (VSMCs) is closely involved in the initiation and progression of cardiovascular complications, such as atherosclerosis. Here we investigated whether ...
DOI:
10.1016/j.lfs.2018.03.036

51. AttrActin’ Attention to Early Mouse Development

Author:
Tristan Frum; Amy Ralston
Source:
Cell 2018 v.173 no.3 pp. 544-545
ISSN:
0092-8674
Subject:
actin; embryo (animal); image analysis; mice
Abstract:
... A new study by Zenker et al. uses time-lapse imaging to discover how dynamic actin movements contribute to epithelialization of living mouse embryos. Together with work from other labs, this study presents exciting new ways to think about the emergence of cell fates during mammalian development. ...
DOI:
10.1016/j.cell.2018.03.078

52. Bacterial metabolite S-equol modulates glucagon-like peptide-1 secretion from enteroendocrine L cell line GLUTag cells via actin polymerization

Author:
Kazuki Harada; Shoko Sada; Hidekazu Sakaguchi; Mai Takizawa; Rika Ishida; Takashi Tsuboi
Source:
Biochemical and biophysical research communications 2018 v.501 no.4 pp. 1009-1015
ISSN:
0006-291X
Subject:
actin; calcium; cell lines; estrogen receptors; exocytosis; glucagon-like peptide 1; immunocytochemistry; insulin secretion; islets of Langerhans; isoflavones; metabolites; mice; microfilaments; polymerization; soybeans
Abstract:
... S-equol is one of gut bacterial metabolites produced from soybean isoflavone daizein. While S-equol is known to promote glucose-induced insulin secretion from pancreatic β cells, whether S-equol affects glucagon-like peptide-1 (GLP-1) secretion from enteroendoceine L cells remains unclear. Here we assessed the effect of S-equol on GLP-1 secretion from mouse enteroendocrine L cell line GLUTag cells ...
DOI:
10.1016/j.bbrc.2018.05.100

53. Basic properties of ATP-induced myosin head movement in hydrated myosin filaments, studied using the gas environmental chamber

Author:
H. Sugi; T. Akimoto; S. Chaen
Source:
Micron 2018 v.112 pp. 15-25
ISSN:
0968-4328
Subject:
X-ray diffraction; actin; adenosine diphosphate; adenosine triphosphate; hydrolysis; microfilaments; muscle contraction; myosin
Abstract:
... Although more than 50 years have passed since the monumental discovery of Huxley and Hanson that muscle contraction results from relative sliding between actin and myosin filaments, coupled with ATP hydrolysis, the mechanism underlying the filament sliding still remains to be a mystery. It is generally believed that the myofilament sliding is caused by cyclic attachment-detachment between myosin h ...
DOI:
10.1016/j.micron.2018.06.003

54. Basic properties of ATP-induced myosin head movement in hydrated myosin filaments, studied using the gas environmental chamber

Author:
H Sugi; T Akimoto; S Chaen
Source:
Micron 2018 v.113 pp. 48-60
ISSN:
0968-4328
Subject:
X-ray diffraction; actin; adenosine diphosphate; adenosine triphosphate; hydrolysis; microfilaments; muscle contraction; myosin
Abstract:
... Although more than 50 years have passed since the monumental discovery of Huxley and Hanson that muscle contraction results from relative sliding between actin and myosin filaments, coupled with ATP hydrolysis, the mechanism underlying the filament sliding still remains to be a mystery. It is generally believed that the myofilament sliding is caused by cyclic attachment-detachment between myosin h ...
DOI:
10.1016/j.micron.2018.06.005

55. Beta-tubulin and Actin gene phylogeny supports Phaeoacremonium ovale as a new species from freshwater habitats in China

Author:
Shi-Ke Huang; Rajesh Jeewon; Kevin D. Hyde; D. Jayarama Bhat; Putarak Chomnunti; Ting-Chi Wen
Source:
MycoKeys 2018 v.41 pp. 1-15
ISSN:
1314-4049
Subject:
Phaeoacremonium; actin; anamorphs; ascomata; ascospores; conidia; data collection; freshwater; fungi; genes; habitats; new species; phylogeny; strains; tubulin; China
Abstract:
... A new species of Phaeoacremonium, P. ovale (Togniniaceae), was isolated during a diversity study of freshwater fungi from Yunnan Province in China. Morphological and cultural studies of the fungus were carried out and its sexual and asexual morphs (holomorph) are introduced herein. This species is characterised by peculiar long-necked, semi-immersed ascomata with oval to ellipsoid ascospores and e ...
DOI:
10.3897/mycokeys.41.27536

56. Biochemical alterations in inflammatory reactive chondrocytes: evidence for intercellular network communication

Author:
Eva Skiöldebrand; Anna Thorfve; Ulrika Björklund; Pegah Johansson; Ruth Wickelgren; Anders Lindahl; Elisabeth Hansson
Source:
Heliyon 2018 v.4 no.1 pp. e00525
ISSN:
2405-8440
Subject:
Toll-like receptor 4; Western blotting; actin; astrocytes; calcium; cartilage; chondrocytes; fibroblasts; fluorescent dyes; gap junctions; giant cells; glutamic acid; inflammation; lipopolysaccharides; matrix metalloproteinase 13; phalloidine
Abstract:
... Chondrocytes are effectively involved in the pathophysiological processes of inflammation in joints. They form cellular processes in the superficial layer of the articular cartilage and form gap junction coupled syncytium to facilitate cell-to-cell communication. However, very little is known about their physiological cellular identity and communication. The aim with the present work is to evaluat ...
DOI:
10.1016/j.heliyon.2018.e00525
PubMed:
29560438
PubMed Central:
PMC5857518

57. Biology, pathogenicity, and haplotype analyses of Colletotrichum cliviae: a novel soybean anthracnose agent in warm tropical areas

Author:
Moab D. Dias; Maria Esther N. Fonseca; Justino J. Dias-Neto; Maria D. M. Santos; Gabriel M. Pandolfo; Leonardo S. Boiteux; Adalberto C. Café-Filho
Source:
Tropical plant pathology 2018 v.43 no.5 pp. 439-451
ISSN:
1983-2052
Subject:
Camellia sinensis; Colletotrichum truncatum; actin; anthracnose; crop rotation; cultivars; fungi; genomics; glyceraldehyde-3-phosphate dehydrogenase; haplotypes; host range; hosts; pathogenicity; seeds; single nucleotide polymorphism; soybeans; tropics; tubulin; Brazil
Abstract:
... Soybean anthracnose is a complex disease, comprising isolates of Colletotrichum truncatum and at least six other species. In Brazil, C. cliviae was recently reported as a novel causal agent of soybean anthracnose but very little information is available about the C. cliviae-soybean pathosystem. Here we report the occurrence of C. cliviae in commercial soybean fields in the Tocantins State, TO (Nor ...
DOI:
10.1007/s40858-018-0249-6

58. Biophysical characterization of actin bundles generated by the Chlamydia trachomatis Tarp effector

Author:
Susmita Ghosh; Jinho Park; Mitchell Thomas; Edgar Cruz; Omar Cardona; Hyeran Kang; Travis Jewett
Source:
Biochemical and biophysical research communications 2018 v.500 no.2 pp. 423-428
ISSN:
0006-291X
Subject:
Chlamydia trachomatis; actin; bundling; crosslinking; fluorescence; humans; mechanical properties; microfilaments; microscopy; mutants; phosphoproteins; proline
Abstract:
... Chlamydia trachomatis entry into host cells is mediated by pathogen-directed remodeling of the actin cytoskeleton. The chlamydial type III secreted effector, translocated actin recruiting phosphoprotein (Tarp), has been implicated in the recruitment of actin to the site of internalization. Tarp harbors G-actin binding and proline rich domains required for Tarp-mediated actin nucleation as well as ...
DOI:
10.1016/j.bbrc.2018.04.093
PubMed:
29660331
PubMed Central:
PMC5928783

59. Bitter tastant quinine modulates glucagon-like peptide-1 exocytosis from clonal GLUTag enteroendocrine L cells via actin reorganization

Author:
Kazuki Harada; Hidekazu Sakaguchi; Shoko Sada; Rika Ishida; Yuki Hayasaka; Takashi Tsuboi
Source:
Biochemical and biophysical research communications 2018 v.500 no.3 pp. 723-730
ISSN:
0006-291X
Subject:
actin; calcium; calcium channels; cyclic AMP; endoplasmic reticulum; exocytosis; fluorescence microscopy; forskolin; gastrointestinal system; glucagon-like peptide 1; glucose; homeostasis; immunocytochemistry; messenger RNA; mice; plasma membrane; polymerization; quinine; receptors; secretion
Abstract:
... Enteroendocrine L cells in the gastrointestinal tract secrete glucagon-like peptide-1 (GLP-1), which plays an important role in glucose homeostasis. Here we investigated the effect of bitter tastant quinine on GLP-1 secretion using clonal GLUTag mouse enteroendocrine L cells. We found that GLUTag cells expressed putative quinine receptors at mRNA levels. Although application of quinine resulted in ...
DOI:
10.1016/j.bbrc.2018.04.143

60. Blockage of AKAP12 accelerates angiotensin II (Ang II)-induced cardiac injury in mice by regulating the transforming growth factor β1 (TGF-β1) pathway

Author:
Yong Li; Qiu-Hua Yu; Ying Chu; Wei-Min Wu; Jian-Xiang Song; Xiao-Bo Zhu; Qiang Wang
Source:
Biochemical and biophysical research communications 2018 v.499 no.2 pp. 128-135
ISSN:
0006-291X
Subject:
Western blotting; actin; angiotensin II; apoptosis; autophagy; blood; fibrosis; gene expression regulation; heart; heart failure; hypertension; immunohistochemistry; inflammation; mice; muscles; oxidative stress; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; scaffolding proteins; staining; transcription (genetics); transforming growth factor beta 1
Abstract:
... Hypertension is a multifactorial chronic inflammatory disease that leads to cardiac remodeling. A-kinase anchor protein 12 (AKAP12) is a scaffolding protein that has multiple functions in various biological events, including the regulation of vessel integrity and differentiation of neural barriers in blood. However, the role of AKAP12 in angiotensin II (Ang II)-induced cardiac injury remains uncle ...
DOI:
10.1016/j.bbrc.2018.02.200

61. Bromelain Kinetics and Mechanism on Myofibril from Golden Pomfret (Trachinotus blochii)

Author:
Xiao Feng; Shasha Hang; Yige Zhou; Qin Liu; Hongshun Yang
Source:
Journal of food science 2018 v.83 no.8 pp. 2148-2158
ISSN:
0022-1147
Subject:
Bramidae; Trachinotus blochii; actin; alanine; atomic force microscopy; bromelains; confocal laser scanning microscopy; consumer acceptance; enzyme kinetics; fish; fish cakes; fish meat; free amino acids; glutamic acid; hydrolysis; kinetics; lysine; meat; methionine; myosin heavy chains; myosin light chains; nanomaterials; ornithine; peptides; pineapples; polyacrylamide gel electrophoresis; processing waste; protein aggregates; protein hydrolysates; tenderizing; texture; troponin C
Abstract:
... Bromelain was used to tenderize golden pomfrets (Trachinotus blochii). The enzyme kinetic model was x=2.447×ln[1+(1332.21×E0S0−1.74)t], which indicated that the degree of hydrolysis (DH, x) was dependent on hydrolysis time (t), the initial concentration of myofibril (S₀) and bromelain (E₀). The relationship between the overall hydrolysis rate (v), S₀, E₀, and t is demonstrated as: v=(16.50(E0S0)−1 ...
DOI:
10.1111/1750-3841.14212

62. Butylidenephthalide abrogates the myofibroblasts activation and mesenchymal transdifferentiation in oral submucous fibrosis

Author:
Tzu‐Rong Su; Yi‐Wen Liao; Pei‐Ling Hsieh; Lo‐Lin Tsai; Chih‐Yuan Fang; Taichen Lin; Yu‐Hsien Lee; Horng‐Jyh Harn; Cheng‐Chia Yu
Source:
Environmental toxicology 2018 v.33 no.6 pp. 686-694
ISSN:
1520-4081
Subject:
Angelica sinensis; actin; betel nut; collagen; cytotoxicity; fibroblasts; fibronectins; fibrosis; gels; ingredients; mastication; mouth; muscles
Abstract:
... Oral submucous fibrosis (OSF) is a premalignant disorder in the oral cavity, and areca nut chewing habit has been implicated in the persistent activation of myofibroblasts and the subsequent fibrosis. Therefore, it is critical to ameliorate the excessive activities of myofibroblasts prior to the malignant transformation of OSF. In the current study, we evaluated the cytotoxicity of butylidenephtha ...
DOI:
10.1002/tox.22557

63. Can Strain Dependent Inhibition of Cross-Bridge Binding Explain Shifts in Optimum Muscle Length?

Author:
N C Holt; C D Williams
Source:
Integrative and comparative biology 2018 v.58 no.2 pp. 174-185
ISSN:
1557-7023
Subject:
actin; compliance; contractile proteins; muscles; myosin; skeletal muscle; tropomyosins
Abstract:
... Skeletal muscle force is generated by cross-bridge interactions between the overlapping contractile proteins, actin and myosin. The geometry of this overlap gives us the force–length relationship in which maximum isometric force is generated at an intermediate, optimum, length. However, the force–length relationship is not constant; optimum length increases with decreasing muscle activation. This ...
DOI:
10.1093/icb/icy050
PubMed:
29873724
PubMed Central:
PMC6104710

64. Canine Gastrointestinal Spindle Cell Tumors Efficiently Diagnosed by Tissue Microarray-Based Immunohistochemistry

Author:
Valeria Pellegrino; Luisa V. Muscatello; Giuseppe Sarli; Giancarlo Avallone
Source:
Veterinary pathology v.55 no.5 pp. 678-681
ISSN:
1544-2217
Subject:
actin; animal pathology; cost effectiveness; desmin; dog diseases; dogs; gastrointestinal neoplasms; immunohistochemistry; microarray technology; muscles; smooth muscle
Abstract:
... Tissue microarray (TMA) is a time- and cost-saving technique allowing the simultaneous immunohistochemical evaluation of multiple tissue samples. The aim of this study was to assess the efficacy of TMA at classifying canine gastrointestinal spindle cell tumors as gastrointestinal stromal tumor (GIST), smooth muscle tumor (SMT), and non-GIST/non-SMT based on the expression of α-smooth muscle actin ...
DOI:
10.1177/0300985818777793

65. Canker disease caused by Chrysoporthe doradensis and C. cubensis on Eucalyptus sp. and Tibouchina spp. in Brazil

Author:
Thaissa P. F. Soares; Maria A. Ferreira; Reginaldo G. Mafia; Leonardo S. S. Oliveira; Charles S. Hodges; Acelino C. Alfenas
Source:
Tropical plant pathology 2018 v.43 no.4 pp. 314-322
ISSN:
1983-2052
Subject:
Chrysoporthe cubensis; Eucalyptus grandis; Tibouchina; actin; branches; breeding programs; clones; conidia; death; genomics; hosts; hybrids; pathogenicity; screening; sequence analysis; tree trunk; tubulin; woody plants; Brazil
Abstract:
... Chrysoporthe species are known to cause canker on hosts belonging to Myrtaceae and Melastomataceae families. Cankers occur on tree trunks and branches and may reduce growth and lead to plant death. In this study, the incidence and pathogenicity of Chrysoporthe cubensis and other species causing canker on Eucalyptus sp., Tibouchina heteromalla and T. granulosa were examined. The isolates were colle ...
DOI:
10.1007/s40858-018-0238-9

66. Canonical signaling and nuclear activity of mTOR—a teamwork effort to regulate metabolism and cell growth

Author:
Vincent Giguère
Source:
FEBS journal 2018 v.285 no.9 pp. 1572-1588
ISSN:
1742-464X
Subject:
actin; autophagy; biogenesis; carbohydrates; cell growth; chromatin; energy; growth factors; lipid metabolism; mammals; messenger RNA; microtubules; nutrient availability; protein degradation; rapamycin; ribosomes; teams; transcription (genetics); transcription factors
Abstract:
... Mechanistic (or mammalian) target of rapamycin (mTOR) is a kinase that regulates almost all functions related to cell growth and metabolism in response to extra‐ and intracellular stimuli, such as availability of nutrients, the presence of growth factors, or the energy status of the cell. As part of two distinct protein complexes, mTORC1 and mTORC2, the kinase has been shown to influence cell grow ...
DOI:
10.1111/febs.14384

67. Cantharidin suppresses HCT116 colorectal carcinoma cell proliferation and migration by changing the cytoskeleton structure

Author:
Xin Zhang; Tongtong Sui; Qixiang Ma; Haozhen Shao; Xiaowei Hu; Honghao Sheng; Zhitao Ma; Guangbin Luo
Source:
Journal of traditional Chinese medical sciences 2018 v.5 no.3 pp. 302-309
ISSN:
2095-7548
Subject:
actin; antineoplastic activity; apoptosis; cancer therapy; cantharidin; cell movement; cell proliferation; colorectal neoplasms; flow cytometry; humans; interphase; metastasis; natural toxicants; neoplasm cells; propidium
Abstract:
... Cantharidin (CTD), a natural toxin produced from Chinese blister beetles, has extensive anti-tumor activity. The present study investigated the effect of CTD on a human colon cancer cell line to elucidate potential new insights regarding the mechanism(s) through which CTD exerts its anti-tumor effects.The inhibitory effect of CTD on human colon cancer HCT116 cells was evaluated using the IncuCyte ...
DOI:
10.1016/j.jtcms.2018.07.004

68. Carapace epithelia are rich in large filamentous actin bundles in Daphnia magna, Daphnia pulex, and Sida crystallina (Crustacea: Cladocera)

Author:
Arne K. Christensen; Nana G. Owusu; David Jean‐Louis
Source:
Invertebrate biology 2018 v.137 no.1 pp. 49-59
ISSN:
1077-8306
Subject:
Daphnia magna; Daphnia pulex; Sida crystallina; actin; adults; confocal microscopy; epithelium; fluorescence; genes; instars; integument; juveniles; organelles; plankton
Abstract:
... Cladocerans (water fleas) are planktonic crustaceans that typically have a bivalved carapace. Each valve of the carapace consists of two cuticle‐secreting epithelial layers that are separated by a hemolymphatic chamber and joined by pillar structures. Ultrastructural analyses in several species of Cladocera have shown that the carapace epithelia and pillars contain filamentous structures of unknow ...
DOI:
10.1111/ivb.12204

69. Cations Modulate Actin Bundle Mechanics, Assembly Dynamics, and Structure

Author:
Nicholas Castaneda; Tianyu Zheng; Hector J. Rivera-Jacquez; Hyun-Ju Lee; Jaekyung Hyun; Alexander Balaeff; Qun Huo; Hyeran Kang
Source:
Journal of physical chemistry 2018 v.122 no.14 pp. 3826-3835
ISSN:
1520-5207
Subject:
actin; cations; electrostatic interactions; fluorescence microscopy; light scattering; mechanical properties; microfilaments; molecular dynamics; simulation models; transmission electron microscopy
Abstract:
... Actin bundles are key factors in the mechanical support and dynamic reorganization of the cytoskeleton. High concentrations of multivalent counterions promote bundle formation through electrostatic attraction between actin filaments that are negatively charged polyelectrolytes. In this study, we evaluate how physiologically relevant divalent cations affect the mechanical, dynamic, and structural p ...
DOI:
10.1021/acs.jpcb.8b00663

70. Ca²⁺- and Mg²⁺-Induced Conformational and Rheological Changes of Actomyosin Extracted from Fresh and Freeze-Thaw Tilapia

Author:
Jirawat Yongsawatdigul; Sornchai Sinsuwan
Source:
Journal of aquatic food product technology 2018 v.27 no.10 pp. 1063-1077
ISSN:
1547-0636
Subject:
Ca2-transporting ATPase; Oreochromis niloticus; actin; adenosinetriphosphatase; calcium; denaturation; fish; freeze-thaw cycles; frozen fish; gelation; hydrophobic bonding; ions; magnesium; modulus of elasticity; myosin heavy chains; polyacrylamide gel electrophoresis; raw fish; temperature
Abstract:
... The conformational changes of natural actomyosins prepared from fresh and freeze-thaw tilapia (Oreochromis niloticus) in the presence of Ca²⁺ or Mg²⁺ were investigated. The Ca²⁺-activated adenosine triphosphatase (Ca²⁺-ATPase) activities of actomyosins extracted from fresh and freeze-thaw fish were comparable (p > 0.05). The denaturation temperatures (Td) of actomyosins extracted from fresh fish w ...
DOI:
10.1080/10498850.2018.1534301

71. Cells may feel a hard substrate even on a grafted layer of soft hydrogel

Author:
Shuhao Wang; Fei Zan; Yu Ke; Gang Wu
Source:
Journal of materials chemistry 2018 v.6 no.12 pp. 1734-1743
ISSN:
2050-7518
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; actin; adhesion; atomic force microscopy; biocompatible materials; cadherins; cell lines; chemical structure; chondrocytes; genes; glass; humans; hydrogels; integrins; polyhydroxyethyl methacrylates; polymerization; tissue engineering
Abstract:
... Introducing or grafting molecules onto biomaterial surfaces to regulate cell destination via biophysical cues is one of the important steps for biomaterial design in tissue engineering. Understanding how cells feel the substrate makes it easier to learn the mechanism behind cell–material interaction. In this study, on a glass substrate, we constructed poly-phenoxyethyl methacrylate (PHEMA) brushes ...
DOI:
10.1039/c7tb02967e

72. Cellular interactions with hydrogel microfibers synthesized via interfacial tetrazine ligation

Author:
Shuang Liu; Axel C. Moore; Aidan B. Zerdoum; Han Zhang; Samuel L. Scinto; He Zhang; Liang Gong; David L. Burris; Ayyappan K. Rajasekaran; Joseph M. Fox; Xinqiao Jia
Source:
Biomaterials 2018 v.180 pp. 24-35
ISSN:
0142-9612
Subject:
actin; cell culture; cell movement; crosslinking; extracellular matrix; fibroblasts; hydrogels; microstructure; oil-water interface; peptides; polymerization; tissue repair
Abstract:
... Fibrous proteins found in the natural extracellular matrix (ECM) function as host substrates for migration and growth of endogenous cells during wound healing and tissue repair processes. Although various fibrous scaffolds have been developed to recapitulate the microstructures of the native ECM, facile synthesis of hydrogel microfibers that are mechanically robust and biologically active have bee ...
DOI:
10.1016/j.biomaterials.2018.06.042
PubMed:
30014964
PubMed Central:
PMC6091885

73. Changes in Skeletal Muscle and Body Weight on Sleeping Beauty Transposon-Mediated Transgenic Mice Overexpressing Pig mIGF-1

Author:
Bo Gao; Wei Wang; Han Wu; Cai Chen; Dan Shen; Saisai Wang; Wei Chen; Li Zhang; Shuheng Chan; Chengyi Song
Source:
Biochemical genetics 2018 v.56 no.4 pp. 341-355
ISSN:
0006-2928
Subject:
Cytomegalovirus; actin; adults; animal models; body weight; fibroblasts; gene expression; genetic vectors; green fluorescent protein; hypertrophy; insulin-like growth factor I; kidney cells; males; messenger RNA; mice; muscle development; muscles; myoblasts; plasmids; reporter genes; skeletal muscle; swine; transgenes; transgenic animals; transposons
Abstract:
... Insulin-like growth factor (IGF-I) is an important growth factor in mammals, but the functions of the local muscle-specific isoform of insulin-like growth factor 1 (mIGF-1) to skeletal muscle development have rarely been reported. To determine the effect of pig mIGF-1 on body development and muscle deposition in vivo and to investigate the molecular mechanisms, the transgenic mouse model was gener ...
DOI:
10.1007/s10528-018-9848-7
PubMed:
29470680
PubMed Central:
PMC6028850

74. Characterization of Fusarium oxysporum f. sp. melongenae isolates from Turkey with ISSR markers and DNA sequence analyses

Author:
Hacer Handan Altinok; Canan Can; Mahmut Alper Altinok
Source:
European journal of plant pathology 2018 v.150 no.3 pp. 609-621
ISSN:
0929-1873
Subject:
DNA; Fusarium oxysporum; Fusarium wilt; actin; eggplants; genetic variation; greenhouses; internal transcribed spacers; loci; microsatellite repeats; monophyly; pathogens; peptide elongation factors; population structure; sequence analysis; transcription (genetics); translation (genetics); trees; Turkey (country)
Abstract:
... Fusarium oxysporum f. sp. melongenae (Fomg), causal agent of Fusarium wilt of eggplant, is a serious pathogen in open fields and greenhouses. Inter-simple sequence repeat (ISSR) banding profiles, sequence analyses of inter-transcribed-spacer (ITS), translation elongation factor 1-alpha (TEF-1α), and actin (actA) DNA regions were employed in this study to determine genetic diversity and population ...
DOI:
10.1007/s10658-017-1305-7

75. Characterization of myofibrillar adenosine triphosphatase activity and liberation of actin from myofibrils upon heating chicken breast meat

Author:
Masanori Matsuishi; Yoshitaka Eda; Emi Saito; Shohei Yamamoto; Kenji Kanamori; Yuto Goto; Yutaro Kobayashi; Akihiro Okitani
Source:
Animal science journal = 2018 v.89 no.3 pp. 597-605
ISSN:
1344-3941
Subject:
actin; adenosinetriphosphatase; breast meat; chickens; cooking; denaturation; enzyme activity; heat stability; minced meat; myofibrils; myosin; temperature
Abstract:
... Denaturation of actin and myosin in myofibrils induced by heating at 50°C was investigated to reveal the mechanism of irreversible liberation of actin from myofibrils on heating at lower temperatures than conventional cooking. Denaturation of these proteins was determined by Mg²⁺‐ATPase (adenosine triphosphatase) and Ca²⁺‐ATPase activities. When minced meat was heated for 20 min, actin was liberat ...
DOI:
10.1111/asj.12960

76. Characterization of ohmic heating and sous-vide treatment of scallops: Analysis of electrical conductivity and the effect of thermal protein denaturation on quality attribute changes

Author:
Yvan Llave; Kimihiko Morinaga; Mika Fukuoka; Noboru Sakai
Source:
Innovative food science & emerging technologies 2018 v.50 pp. 112-123
ISSN:
1466-8564
Subject:
actin; catering; differential scanning calorimetry; electrical conductivity; energy; kinetics; kitchens; muscles; ohmic heating; processed foods; processing time; product safety; protein denaturation; restaurants; scallops; sous vide; temperature
Abstract:
... A 20 kHz ohmic heating (OH) and two sous-vide (SV) treatments of scallop muscles were characterized, and their effects on thermal protein denaturation (TPD) and quality attribute changes were evaluated. TPD was analyzed by differential scanning calorimetry and estimated by kinetic analysis for several thermal schedules with a dynamic method. The OH process was evaluated by analyzing the electrical ...
DOI:
10.1016/j.ifset.2018.09.007

77. Characterizing interaction forces between actin and proteins of the tropomodulin family reveals the presence of the N-terminal actin-binding site in leiomodin

Author:
Baran Arslan; Mert Colpan; Kevin T. Gray; Nehal I. Abu-Lail; Alla S. Kostyukova
Source:
Archives of biochemistry and biophysics 2018 v.638 pp. 18-26
ISSN:
0003-9861
Subject:
actin; atomic force microscopy; mutation; polymerization
Abstract:
... Tropomodulin family of proteins includes several isoforms of tropomodulins (Tmod) and leiomodins (Lmod). These proteins can sequester actin monomers or nucleate actin polymerization. Although it is known that their actin-binding properties are isoform-dependent, knowledge on how they vary in strengths of interactions with G-actin is missing. While it is confirmed in many studies that Tmods have tw ...
DOI:
10.1016/j.abb.2017.12.005
PubMed:
29223925
PubMed Central:
PMC5762396

78. Chitosan and thiolated chitosan: Novel therapeutic approach for preventing corneal haze after chemical injuries

Author:
Forouhe Zahir-Jouzdani; Mirgholamreza Mahbod; Masoud Soleimani; Faezeh Vakhshiteh; Ehsan Arefian; Saeed Shahosseini; Rasoul Dinarvand; Fatemeh Atyabi
Source:
Carbohydrate polymers 2018 v.179 pp. 42-49
ISSN:
0144-8617
Subject:
actin; angiogenesis; chitosan; collagen; cornea; extracellular matrix; fibroblasts; fibronectins; humans; immunohistochemistry; interleukin-6; mice; muscles; nanoparticles; platelet-derived growth factor; polymers; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; therapeutics; transforming growth factor beta 1; vascular endothelial growth factors; vision
Abstract:
... Corneal haze, commonly caused by deep physical and chemical injuries, can greatly impair vision. Growth factors facilitate fibroblast proliferation and differentiation, which leads to haze intensity. In this study, the potential effect of chitosan (CS) and thiolated-chitosan (TCS) nanoparticles and solutions on inhibition of fibroblast proliferation, fibroblast to myofibroblast differentiation, ne ...
DOI:
10.1016/j.carbpol.2017.09.062

79. Cholesterol inhibits entotic cell-in-cell formation and actomyosin contraction

Author:
Banzhan Ruan; Bo Zhang; Ang Chen; Long Yuan; Jianqing Liang; Manna Wang; Zhengrong Zhang; Jie Fan; Xiaochen Yu; Xin Zhang; Zubiao Niu; You Zheng; Songzhi Gu; Xiaoqing Liu; Hongli Du; Jufang Wang; Xianwen Hu; Lihua Gao; Zhaolie Chen; Hongyan Huang; Xiaoning Wang; Qiang Sun
Source:
Biochemical and biophysical research communications 2018 v.495 no.1 pp. 1440-1446
ISSN:
0006-291X
Subject:
actin; cell membranes; cholesterol; humans; myosin light chains; neoplasms; phosphatidylethanolamines; phosphorylation; screening; transfection
Abstract:
... Cell-in-cell structure is prevalent in human cancer, and associated with several specific pathophysiological phenomena. Although cell membrane adhesion molecules were found critical for cell-in-cell formation, the roles of other membrane components, such as lipids, remain to be explored. In this study, we attempted to investigate the effects of cholesterol and phospholipids on the formation of cel ...
DOI:
10.1016/j.bbrc.2017.11.197

80. Chrysin Inhibits Advanced Glycation End Products-Induced Kidney Fibrosis in Renal Mesangial Cells and Diabetic Kidneys

Author:
Eun-Jung Lee; Min-Kyung Kang; Dong Yeon Kim; Yun-Ho Kim; Hyeongjoo Oh; Young-Hee Kang
Source:
Nutrients 2018 v.10 no.7 pp. -
ISSN:
2072-6643
Subject:
actin; advanced glycation end products; bees; chrysin; collagen; diabetic nephropathy; fibrosis; glucose; glycation; herbs; in vitro studies; in vivo studies; kidneys; metalloproteinases; mice; models; muscles; noninsulin-dependent diabetes mellitus; propolis; protein deposition; serum albumin; tissues; transforming growth factor beta 1
Abstract:
... Advanced glycation end products (AGEs) play a causative role in the development of diabetic nephropathy via induction of matrix protein deposition in kidneys. This study investigated inhibitory effects of chrysin, present in bee propolis and herbs, on glomerulosclerosis in db/db mice and AGEs-exposed renal mesangial cells. The in vivo study explored the demoting effects of 10 mg/kg chrysin on glom ...
DOI:
10.3390/nu10070882
PubMed:
29987200
PubMed Central:
PMC6073220

81. Citrullinated histone 3 causes endothelial barrier dysfunction

Author:
Jamie E. Meegan; Xiaoyuan Yang; Richard S. Beard; Melanie Jannaway; Victor Chatterjee; Thomas E. Taylor-Clark; Sarah Y. Yuan
Source:
Biochemical and biophysical research communications 2018 v.503 no.3 pp. 1498-1502
ISSN:
0006-291X
Subject:
actin; adenylate cyclase; biomarkers; cytoskeleton; forskolin; histones; inflammation; neutrophils; sepsis (infection); signal transduction
Abstract:
... Circulating components of neutrophil extracellular traps (NETs), especially histones, are associated with tissue injury during inflammatory conditions like sepsis. Commonly used as a NET biomarker, citrullinated histone 3 (H3Cit) may also functionally contribute to the NET-associated inflammatory response. To this end, we sought to examine the role of H3Cit in mediating microvascular endothelial b ...
DOI:
10.1016/j.bbrc.2018.07.069
PubMed:
30029877
PubMed Central:
PMC6119499

82. Clathrin-mediated endocytosis regulates fMLP-mediated neutrophil polarization

Author:
Xinyu Tan; Mingzhi Luo; Allen P. Liu
Source:
Heliyon 2018 v.4 no.9 pp. e00819
ISSN:
2405-8440
Subject:
actin; chemoattractants; clathrin; endocytosis; neutrophils; peptide receptors
Abstract:
... A cell's ability to establish polarization is one of the key steps in directional migration. Upon the addition of a chemoattractant, N-formylmethionyl-leucyl-phenylalanine (fMLP), neutrophils rapidly develop a front end marked by a wide and dense actin network which is a feature of cell polarization. Despite a general understanding of bi-directional crosstalk between endocytosis and polarization, ...
DOI:
10.1016/j.heliyon.2018.e00819
PubMed:
30263974
PubMed Central:
PMC6157066

83. Clostridioides difficile

Author:
Brindar K. Sandhu; Shonna M. McBride
Source:
Trends in microbiology 2018 v.26 no.12 pp. 1049-1050
ISSN:
0966-842X
Subject:
Clostridium difficile; actin; bacteria; bile acids; cell death; colon; death; diarrhea; health care costs; pathogens; people; polymerization; spores; sporulation; toxins; United States
Abstract:
... [Display omitted]Clostridioides difficile is a spore-forming, anaerobic, intestinal pathogen that causes severe diarrhea that can lead to death. In 2011, C. difficile infected ∼500000 people in the USA and killed ∼29000 people. C. difficile infection (CDI) is the most common healthcare-related infection in the USA, leading to increased healthcare costs of $4.8 billion. This pathogen transmits via ...
DOI:
10.1016/j.tim.2018.09.004
PubMed:
30297117
PubMed Central:
PMC6637408

84. Cnn3 regulates neural tube morphogenesis and neuronal stem cell properties

Author:
Dirk Junghans; Sebastian Herzog
Source:
FEBS journal 2018 v.285 no.2 pp. 325-338
ISSN:
1742-464X
Subject:
abnormal development; actin; beta catenin; brain; cadherins; cytoskeleton; dendrites; embryogenesis; immunohistochemistry; mammals; morphogenesis; mutants; plasticity; signal transduction; stem cells
Abstract:
... Calponin 3 (Cnn3) is a member of the Cnn family of actin‐binding molecules that is highly expressed in the mammalian brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. However, little is known about the role of Cnn3 during embryonic development. In this study, we analyzed mutant animals deficient in ...
DOI:
10.1111/febs.14338

85. Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton

Author:
Stefano Perni; Lirong Yang; Emily Callard Preedy; Polina Prokopovich
Source:
Journal of colloid and interface science 2018 v.531 pp. 410-420
ISSN:
0021-9797
Subject:
actin; alizarin; apoptosis; cobalt; cytoskeleton; fluorescence; humans; mineralization; mitochondria; nanometals; nanoparticles; osteoblasts; staining; titanium
Abstract:
... We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation outp ...
DOI:
10.1016/j.jcis.2018.07.028

86. Codonopsis tangshen Oliv. Amelioration Effect on Diabetic Kidney Disease Rats Induced by High Fat Diet Feeding Combined with Streptozotocin

Author:
Xian-Yuan Lu; Feng-Hua Zhou; Ya-Qian Dong; Lin-Na Gong; Qing-Yun Li; Lan Tang; Zheng Cai; Jing-Yu He; Meng-Hua Liu
Source:
Natural products and bioprospecting 2018 v.8 no.6 pp. 441-451
ISSN:
2192-2195
Subject:
Codonopsis pilosula subsp. tangshen; actin; basement membrane; blood glucose; blood serum; chemical oxygen demand; collagen; creatinine; diabetes; fibrosis; high fat diet; inflammation; interleukin-1beta; interleukin-6; kidney diseases; lipid metabolism; low density lipoprotein cholesterol; messenger RNA; models; muscles; protective effect; rats; renal function; sclerosis; streptozotocin; transforming growth factor beta 1; triacylglycerols; tumor necrosis factor-alpha; uric acid; weight loss
Abstract:
... Diabetic kidney disease (DKD) is the most serious microvascular complication during the development of diabetes with the characterizations of glomerular basement membrane thickening, mesangial expansion, and glomerular sclerosis, eventually leading to end-stage renal disease. This study aimed to investigate the melioration effect of Codonopisis tangshen Oliv. (COD) on the DKD model, which was esta ...
DOI:
10.1007/s13659-018-0187-5
PubMed:
30387083
PubMed Central:
PMC6224812

87. Collagen-based materials combined with microRNA for repairing cornea wounds and inhibiting scar formation

Author:
Xuan Zhao; Wenjing Song; Yawei Chen; Sa Liu; Li Ren
Source:
Biomaterials science 2018 v.7 no.1 pp. 51-62
ISSN:
2047-4849
Subject:
actin; biocompatibility; collagen; cornea; cytotoxicity; gene expression regulation; genes; immunohistochemistry; mechanical properties; microRNA; muscles; nanogold; staining; stromal cells; tissue repair; transmittance; water content
Abstract:
... The cornea repair materials amniotic membrane, acellular corneal stroma, and natural polymer-based materials have excellent biocompatibility and support epithelization. However, few researchers have focused on corneal stromal wound healing repair with regard to scar formation and transparency improvements. Herein, we introduced nanocomplexes of gold nanoparticles (AuNPs) and microRNA-133b (miR-133 ...
DOI:
10.1039/c8bm01054d

88. Colletotrichum atractylodicola sp. nov.: the anthracnose pathogen of Atractylodes chinensis in China

Author:
Haijiao Xu; Rujun Zhou; Junfan Fu; Yue Yuan; Xinxin Ge; Ulrike Damm
Source:
Mycological progress 2018 v.17 no.3 pp. 393-402
ISSN:
1617-416X
Subject:
Atractylodes lancea; Colletotrichum; DNA; actin; anthracnose; fungi; internal transcribed spacers; leaves; new species; pathogenicity; pathogens; sequence analysis; tubulin; China
Abstract:
... A new anthracnose disease of Atractylodes chinensis was observed in Liaoning province in China. The causal agent was isolated from diseased leaves. Based on morphology and ITS sequence data, it was identified as a Colletotrichum species belonging to the C. destructivum species complex. A multi-locus DNA sequence analysis (ITS, GAPDH, CHS-1, ACT, TUB2) revealed that the fungus represents a new spec ...
DOI:
10.1007/s11557-017-1359-0

89. Colletotrichum cymbidiicola Causes Leaf Spot of Cymbidium ensifolium in Jilin Province, China

Author:
Y. N. Liu; F. R. Mao; H. Zhang; L. B. Wang; S. J. Zheng; J. F. Wang; Q. Lou; J. L. Liu
Source:
Plant disease 2018 v.102 no.7 pp. 1462
ISSN:
0191-2917
Subject:
Colletotrichum; Cymbidium ensifolium; actin; agar; anthracnose; asci; ascospores; conidia; conidiophores; culture media; ethanol; fungi; gardening; glyceraldehyde-3-phosphate dehydrogenase; greenhouses; herbaceous plants; humidity; internal transcribed spacers; leaf spot; leaves; pathogenicity; perithecia; photoperiod; plastic bags; sodium hypochlorite; solar radiation; tubulin; China
Abstract:
... Cymbidium ensifolium (L.) Sw. is a perennial herbaceous plant with high gardening and herbal value. In August 2014, a severe anthracnose disease was observed on C. ensifolium leaves in Changchun (125.24°E, 43.48°N), Jilin Province, China. More than 80% of the plants were symptomatic. In the early stage of disease development, small brown lesions (1 mm in diameter) were visible on infected leaves, ...
DOI:
10.1094/PDIS-08-17-1240-PDN

90. Colletotrichum kakivorum sp. nov., a new leaf spot pathogen of persimmon in Korea

Author:
Seung-Yeol Lee; Hee-Young Jung
Source:
Mycological progress 2018 v.17 no.10 pp. 1113-1121
ISSN:
1617-416X
Subject:
Colletotrichum dematium; actin; appressoria; chitin; conidia; fungi; genes; glyceraldehyde-3-phosphate dehydrogenase; internal transcribed spacers; leaf spot; leaves; loci; new species; pathogenicity; pathogens; persimmons; phylogeny; tubulin; Korean Peninsula
Abstract:
... In this study, a new species within the genus Colletotrichum was isolated from diseased persimmon leaves collected from Sangju and Cheongdo in the Gyeongbuk province of Korea. The disease was characterized by leaf spots with a brown to gray center surrounded by a black ring with a green halo. Black acervuli with setae and numerous curved conidia were observed on the leaf spots. Phylogenetic analys ...
DOI:
10.1007/s11557-018-1424-3

91. Colletotrichum species causing anthracnose on lima bean in Brazil

Author:
Enayra Silva Sousa; Janaíne Rossane Araújo Silva; Iraildes Pereira Assunção; Maruzanete Pereira de Melo; Frederico Monteiro Feijó; Kedma da Silva Matos; Gaus Silvestre de Andrade Lima; José Evando Aguiar Beserra Jr
Source:
Tropical plant pathology 2018 v.43 no.1 pp. 78-84
ISSN:
1983-2052
Subject:
Colletotrichum truncatum; actin; anthracnose; conidia; internal transcribed spacers; leaves; lima beans; pathogenicity; pathogens; phylogeny; pods; polymerase chain reaction; seedlings; taxonomy; tubulin; Brazil
Abstract:
... The taxonomy of the genus Colletotrichum has undergone various changes. These alterations arise from the use of modern molecular tools. Currently, Colletotrichum species are grouped into complexes. Species of Colletotrichum associated with diseases in lima bean have been investigated very little. In Brazil, anthracnose in lima bean has often been associated with Colletotrichum truncatum, but only ...
DOI:
10.1007/s40858-017-0182-0

92. Combined use of bone marrow-derived mesenchymal stromal cells (BM-MSCs) and platelet rich plasma (PRP) stimulates proliferation and differentiation of myoblasts in vitro: new therapeutic perspectives for skeletal muscle repair/regeneration

Author:
Chiara Sassoli; Larissa Vallone; Alessia Tani; Flaminia Chellini; Daniele Nosi; Sandra Zecchi-Orlandini
Source:
Cell and tissue research 2018 v.372 no.3 pp. 549-570
ISSN:
0302-766X
Subject:
actin; blood serum; bone marrow; culture media; mitochondria; muscle development; muscles; myoblasts; myogenin; skeletal muscle; stromal cells; therapeutics; viability
Abstract:
... Satellite cell-mediated skeletal muscle repair/regeneration is compromised in cases of extended damage. Bone marrow mesenchymal stromal cells (BM-MSCs) hold promise for muscle healing but some criticisms hamper their clinical application, including the need to avoid animal serum contamination for expansion and the scarce survival after transplant. In this context, platelet-rich plasma (PRP) could ...
DOI:
10.1007/s00441-018-2792-3

93. Comparative analyses of salivary proteins from the facultative symbiont-infected and uninfected Tetranychustruncatus

Author:
Yu-Xi Zhu; Yue-Ling Song; Hai-Jian Huang; Dian-Shu Zhao; Xue Xia; Kun Yang; Yi-Jia Lu; Xiao-Yue Hong
Source:
Systematic and applied acarology 2018 v.23 no.6 pp. 1027-1042
ISSN:
1362-1971
Subject:
Tetranychus; actin; binding proteins; catalytic activity; endosymbionts; heat shock proteins; isomerases; mites; pests; phosphorylase; phytophagous insects; plant-insect relations; proteinases; ribonucleases; saliva; vitellogenin
Abstract:
... Salivary proteins of herbivorous insects play a central role in plant-insect interactions. Spider mite Tetranychus truncatus is a polyphagous agricultural pest harboring various bacterial symbionts. However, whether endosymbionts infection in spider mite alters the host saliva protein remains largely unknown. Here, by using shotgun LC-MS/MS analysis, we identified and characterized the components ...

94. Comparative gel‐based proteomic analysis of chemically crosslinked complexes in dystrophic skeletal muscle

Author:
Sandra Murphy; Margit Zweyer; Rustam R. Mundegar; Dieter Swandulla; Kay Ohlendieck
Source:
Electrophoresis 2018 v.39 no.14 pp. 1735-1744
ISSN:
0173-0835
Subject:
actin; collagen; crosslinking; cytoskeleton; dystrophin; electrophoresis; gels; genes; mass spectrometry; mitochondria; molecular weight; muscles; muscular dystrophy; oligomerization; pathophysiology; proteomics; skeletal muscle; tubulin; vimentin; wasting syndrome
Abstract:
... Duchenne muscular dystrophy is a highly progressive muscle wasting disease with a complex pathophysiology that is based on primary abnormalities in the dystrophin gene. In order to study potential changes in the oligomerization of high‐molecular‐mass protein complexes in dystrophic skeletal muscle, chemical crosslinking was combined with mass spectrometric analysis. The biochemical stabilization o ...
DOI:
10.1002/elps.201800028
PubMed:
29679381
PubMed Central:
PMC6099379

95. Comparative proteomic profiling of myofibrillar proteins in dry-cured ham with different proteolysis indices and adhesiveness

Author:
M. López-Pedrouso; C. Pérez-Santaescolástica; D. Franco; E. Fulladosa; J. Carballo; C. Zapata; J.M. Lorenzo
Source:
Food chemistry 2018 v.244 pp. 238-245
ISSN:
0308-8146
Subject:
actin; adhesion; biomarkers; cured meats; ham; muscles; myofibrillar proteins; physicochemical properties; proteolysis; proteomics; swine; tandem mass spectrometry; two-dimensional gel electrophoresis
Abstract:
... Excessive proteolysis during dry-cured ham processing may lead to high adhesiveness and consumer dissatisfaction. The aim of this research is to identify biomarkers for proteolysis and adhesiveness. Two hundred biceps femoris porcine muscle samples from Spanish dry-cured ham were firstly evaluated for various physicochemical parameters, including their proteolysis indices and instrumental adhesive ...
DOI:
10.1016/j.foodchem.2017.10.068

96. Conditional reprogrammed human limbal epithelial cells represent a novel in vitro cell model for drug responses

Author:
Ling Wang; Lin Ye; Gaobin Wei; Yu Chen; Lina Ye; Xiaoting Wu; Zhihong Zeng; Yuan Wang; Guogan Yin; Xiang Long; Hui Li
Source:
Biochemical and biophysical research communications 2018 v.499 no.4 pp. 735-742
ISSN:
0006-291X
Subject:
DNA damage; actin; cornea; epithelial cells; epithelium; fluorouracil; humans; karyotyping; lethal dose 50; models; rabbits; staining; swine; toxicity testing
Abstract:
... In this study, we established human limbal epithelial cells (LECs) from normal limbal tissues by using Conditional Reprogramming (CR) technology (refer to CR-LEC cells in this study). We have successfully established CR-LEC cell strains from three human donors (3 out of 3), and normal rabbits (2 out of 2) and pig (1 out of 1) as well. CR-LEC cells sustained a continuous and stable proliferation st ...
DOI:
10.1016/j.bbrc.2018.03.168

97. Contraction-dependent TGF-β1 activation is required for thrombin-induced remodeling in human airway smooth muscle cells

Author:
Zhi-Hui Yao; Hao-Jun Xie; Ya-Lu Yuan; Ya-Ting Huo; Jing Cao; Wen-Yan Lai; Rui-Jun Cai; Yuan-Xiong Cheng
Source:
Life sciences 2018 v.197 pp. 130-139
ISSN:
0024-3205
Subject:
Western blotting; actin; antibodies; asthma; bronchoconstriction; bronchodilators; cell proliferation; coagulation; collagen; cytochalasin D; extracellular matrix; fluorescent antibody technique; gene expression; humans; messenger RNA; myocytes; protein synthesis; reverse transcriptase polymerase chain reaction; smooth muscle; thrombin; transforming growth factor beta 1
Abstract:
... Thrombin is a serine proteinase that is not only involved in coagulation cascade, but also mediates a number of biological responses relevant to tissues repair, and induces bronchoconstriction. TGF-β plays a pivotal role in airway remodeling due to its effects on airway smooth muscle proliferation and extracellular matrix (ECM) deposition. Recently, bronchoconstriction itself is found to constitut ...
DOI:
10.1016/j.lfs.2018.02.012

98. Contribution of myofibril filament disassembly to textural deterioration of ice-stored grass carp fillet: Significance of endogenous proteolytic activity, loss of heat shock protein and dephosphorylation of myosin light chain

Author:
Lihong Ge; Yanshun Xu; Wenshui Xia; Nan Zhao; Qixing Jiang
Source:
Food chemistry 2018 v.269 pp. 511-518
ISSN:
0308-8146
Subject:
Ctenopharyngodon idella; actin; cathepsin B; crosslinking; dephosphorylation; depolymerization; enzyme activity; fish fillets; heat shock proteins; myofibrillar proteins; myosin; phosphorylation; polyacrylamide gel electrophoresis; proteolysis
Abstract:
... To investigate the underlying mechanism of softening of ice-stored grass carp fillet, changes in assembly structure of myofibrillar proteins and potential candidates for regulating this change including myosin regulatory chain phosphorylation, heat shock proteins (Hsp27, Hsp90, αB-crystallin and UNC45) and endogenous protease activity were studied. Comparison of SDS-PAGE pattern of myofibrillar pr ...
DOI:
10.1016/j.foodchem.2018.07.047

99. Controlled Surface Silanization for Actin-Myosin Based Nanodevices and Biocompatibility of New Polymer Resists

Author:
Frida W. Lindberg; Marlene Norrby; Mohammad A. Rahman; Aseem Salhotra; Hideyo Takatsuki; Sören Jeppesen; Heiner Linke; Alf Månsson
Source:
Langmuir 2018 v.34 no.30 pp. 8777-8784
ISSN:
1520-5827
Subject:
actin; adsorption; biocompatibility; contact angle; floors; heavy meromyosin; hydrophobicity; oxygen; polymers; silane; silica
Abstract:
... Molecular motor-based nanodevices require organized cytoskeletal filament guiding along motility-promoting tracks, confined by motility-inhibiting walls. One way to enhance motility quality on the tracks, particularly in terms of filament velocity but also the fraction of motile filaments, is to optimize the surface hydrophobicity. We have investigated the potential to achieve this for the actin-m ...
DOI:
10.1021/acs.langmuir.8b01415

100. Coordination of microtubule acetylation and the actin cytoskeleton by formins

Author:
Jaime Fernández-Barrera; Miguel A. Alonso
Source:
Cellular and molecular life sciences 2018 v.75 no.17 pp. 3181-3191
ISSN:
1420-682X
Subject:
acetylation; acetyltransferases; actin; genes; humans; lysine; mechanical stress; microfilaments; microtubules; neurodegenerative diseases; transcription (genetics); tubulin
Abstract:
... The acetylation of the lysine 40 residue of α-tubulin was described more than 30 years ago and has been the subject of intense research ever since. Although the exact function of this covalent modification of tubulin in the cell remains unknown, it has been established that tubulin acetylation confers resilience to mechanical stress on the microtubules. Formins have a dual role in the fate of the ...
DOI:
10.1007/s00018-018-2855-3