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You searched for: Publication year rev 7982-2018 Remove constraint Publication year rev: 7982-2018 Subject term actin Remove constraint Subject term: actin
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1. 174 Effects of Melatonin on In Vitro Maturation of Bovine Oocytes and Gene Expression in Cumulus Cells: Preliminary Results

Author:
F. C. Castro; L. Schefer; K. L. Schwarz; H. Fernandes; R. C. Botigelli; D. M. Paschoal; C. L. V. Leal
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 226-227
ISSN:
1031-3613
Subject:
RNA; actin; analysis of variance; animal reproduction; anti-inflammatory activity; antioxidant activity; antioxidants; apoptosis; carbon dioxide; cattle; complementary DNA; embryogenesis; follicle-stimulating hormone; gene expression; genes; gentamicin; in vitro culture; luteinizing hormone; melatonin; oocytes; oxidative stress; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; reverse transcription; slaughterhouses; sodium bicarbonate; sodium pyruvate
Abstract:
... Melatonin mediates several processes in animal reproduction and has drawn attention for its potent antioxidant, anti-apoptotic, anti-inflammatory action and, more recently, for its benefits on oocyte maturation and embryo development in vitro. The aim of this study was to assess the effect of melatonin during the in vitro maturation (IVM) on nuclear maturation of bovine oocytes and gene expression ...
DOI:
10.1071/RDv30n1Ab173

2. 209 Imaging the Molecular and Cell Dynamics that Form the Early Mouse Embryo

Author:
N. Plachta
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 245
ISSN:
1031-3613
Subject:
DNA; actin; animal tissues; developmental stages; human fertility; image analysis; mice; microtubules; placenta; pseudopodia; transcription (genetics); transcription factors
Abstract:
... Our goal is to reveal how mammalian cells resolve their fate, shape, and position in the body in real time. Understanding how these decisions are made is critical to realise how embryos form and what problems compromise human fertility. Yet, their real-time control in vivo remains unknown. Because fixed specimens cannot capture cell dynamics, we established imaging technologies to study cells dire ...
DOI:
10.1071/RDv30n1Ab209

3. 24 Evaluation of Latrunculin A for the Activation of Hand-Made Cloning (HMC) Porcine Embryos

Author:
F. K. Castañeda; N. G. Canel; G. V. Landschoot; A. De Stéfano; R. J. Bevacqua; D. F. Salamone
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 151-152
ISSN:
1031-3613
Subject:
actin; adults; air; blastocyst; carbon dioxide; chromosome number; cycloheximide; cytochalasin B; enzymatic treatment; extrusion; fibroblasts; metaphase; microfilaments; oocytes; parthenogenesis; progeny; somatic cells; swine; ultraviolet radiation
Abstract:
... Somatic cell nuclear transfer (SCNT) is an important biotechnological tool. However, production rates of viable offspring remain low. One possible cause of this low efficiency is chromosomal losses during early activation process (Liu et al. 2015 Cell. Reprogram. 17, 463–471). The use of actin inhibitors that block second polar body extrusion during activation protocols might be a strategy to avoi ...
DOI:
10.1071/RDv30n1Ab23

4. 3D Cell-Based High-Content Screening (HCS) Using a Micropillar and Microwell Chip Platform

Author:
Sang-Yun Lee; Il Doh; Do-Hyun Nam; Dong Woo Lee
Source:
Analytical chemistry 2018 v.90 no.14 pp. 8354-8361
ISSN:
1520-6882
Subject:
Western blotting; actin; alginates; antibodies; cell culture; cell viability; cultured cells; drugs; microfilaments; neoplasms; phenotype; screening; staining
Abstract:
... A micropillar/microwell chip platform was applied to develop a 3D cell-based high-content screening (HCS) platform. Previously, 3D cell culture in the micropillar/microwell chip platform was only limited to cell viability measurements in a high-throughput manner. However, an HCS system could provide biological and phenotypic information which was very useful to understand complex biological functi ...
DOI:
10.1021/acs.analchem.7b05328

5. Abiotic stress and tissue-specific reference genes for quantitative reverse transcription PCR analysis in Korean native watermelons, Citrullus lanatus ‘Black-King’ and ‘Speed-Plus-Honey’

Author:
Soo Jin Kim; Il Lae Jung; Hye-Eun Lee; Ji-Hoon Lee
Source:
Han'guk Ŭngyong Saengmyŏng Hwahakhoe chi = 2018 v.61 no.5 pp. 593-598
ISSN:
1738-2203
Subject:
Arabidopsis; Citrullus lanatus; abiotic stress; abscisic acid; actin; cucumbers; cultivars; databases; drought; flowers; gene expression; genes; glyceraldehyde-3-phosphate dehydrogenase; leaves; nucleotide sequences; peptide elongation factors; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ribosomal RNA; salt concentration; screening; seedlings; sodium chloride; ubiquitin-protein ligase; watermelons
Abstract:
... A wide variety of research on watermelon has been conducted, and such studies have been motivated by the published genome sequence database of watermelon. Screening of proper reference genes is the primary step for normalization in gene expression analysis. Based on previous studies conducted on Arabidopsis and cucumber, we selected eight candidate reference genes of ClACT, ClEF1α, ClGAPDH, ClIDH, ...
DOI:
10.1007/s13765-018-0391-3

6. Actin Polymerization Mediated by AtFH5 Directs the Polarity Establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis

Author:
Chang Liu; Yi Zhang; Haiyun Ren
Source:
Molecular plant 2018 v.11 no.11 pp. 1389-1399
ISSN:
1674-2052
Subject:
Arabidopsis; actin; microfilaments; physiological transport; plant reproduction; plasma membrane; pollen; pollen germination; polymerization
Abstract:
... The process of pollen germination is crucial for flowering plant reproduction, but the mechanisms through which pollen grains establish polarity and select germination sites are not well understood. In this study, we report that a formin family protein, AtFH5, is localized to the vesicles and rotates ahead of Lifeact-mEGFP-labeled actin filaments during pollen germination. The translocation of AtF ...
DOI:
10.1016/j.molp.2018.09.004

7. Actin as a cytoskeletal basis for cell architecture and a protein essential for ecdysis in Prorocentrum minimum (Dinophyceae, Prorocentrales)

Author:
Mariia Berdieva; Ilya Pozdnyakov; Olga Matantseva; Nikolay Knyazev; Sergei Skarlato
Source:
Phycological research 2018 v.66 no.2 pp. 127-136
ISSN:
1322-0829
Subject:
Prorocentrum; actin; cell nucleolus; chromosomes; deoxyribonuclease I; ecdysis; electron microscopy; fluorescence; fluorescent labeling; isothiocyanates; microfilaments; microtubules; staining
Abstract:
... The specific cell architecture of prorocentroid dinoflagellates is reflected in the internal cell structure, particularly, in cytoskeleton organization. Cytoskeleton arrangement in a Prorocentrum minimum cell was investigated using fluorescent labeling approaches, electron‐microscopy and immunocytochemical methods. The absence of cortical microtubules was confirmed. Phalloidin – tetramethylrhodami ...
DOI:
10.1111/pre.12214

8. Actin cytoskeleton and sperm function

Author:
Haim Breitbart; Maya Finkelstein
Source:
Biochemical and biophysical research communications 2018 v.506 no.2 pp. 372-377
ISSN:
0006-291X
Subject:
actin; eggs; exocytosis; female reproductive system; mammals; microfilaments; oocytes; polymerization; sperm capacitation; spermatozoa
Abstract:
... For the acquisition of the ability to fertilize the egg, mammalian spermatozoa should undergo a series of biochemical transformations in the female reproductive tract, collectively called capacitation. The capacitated sperm can undergo the acrosomal exocytosis process near or on the oocyte, which allows the spermatozoon to penetrate and fertilize it. One of the main processes in capacitation invol ...
DOI:
10.1016/j.bbrc.2017.11.001

9. Actin dynamics in host–pathogen interaction

Author:
Theresia E. B. Stradal; Mario Schelhaas
Source:
FEBS letters 2018 v.592 no.22 pp. 3658-3669
ISSN:
0014-5793
Subject:
actin; autophagy; exocytosis; guanosinetriphosphatase; host-pathogen relationships; membrane fusion; microfilaments; phagocytosis
Abstract:
... The actin cytoskeleton and Rho GTPase signaling to actin assembly are prime targets of bacterial and viral pathogens, simply because actin is involved in all motile and membrane remodeling processes, such as phagocytosis, macropinocytosis, endocytosis, exocytosis, vesicular trafficking and membrane fusion events, motility, and last but not least, autophagy. This article aims at providing an overvi ...
DOI:
10.1002/1873-3468.13173

10. Actin filaments regulate the adhesion between the plasma membrane and the cell wall of tobacco guard cells

Author:
Qin Yu; Jing-Jing Ren; Lan-Jing Kong; Xiu-Ling Wang
Source:
Protoplasma 2018 v.255 no.1 pp. 235-245
ISSN:
0033-183X
Subject:
actin; cell walls; depolymerization; endocytosis; guard cells; microfilaments; plasma membrane; plasmolysis; stomata; stomatal movement; tobacco
Abstract:
... During the opening and closing of stomata, guard cells undergo rapid and reversible changes in their volume and shape, which affects the adhesion of the plasma membrane (PM) to the cell wall (CW). The dynamics of actin filaments in guard cells are involved in stomatal movement by regulating structural changes and intracellular signaling. However, it is unclear whether actin dynamics regulate the a ...
DOI:
10.1007/s00709-017-1149-1

11. Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei

Author:
Xiaoxi Zhang; Xiaojun Zhang; Jianbo Yuan; Jiangli Du; Fuhua Li; Jianhai Xiang
Source:
Molecular genetics and genomics 2018 v.293 no.2 pp. 479-493
ISSN:
1617-4615
Subject:
Litopenaeus vannamei; Vibrio; White spot syndrome virus; actin; complementary DNA; disease control; hemocytes; multigene family; muscles; phylogeny; postlarvae; sequence analysis; shrimp; skeletal muscle; unigenes
Abstract:
... Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs ...
DOI:
10.1007/s00438-017-1397-y

12. Actin–myosin XI: an intracellular control network in plants

Author:
Zhongrui Duan; Motoki Tominaga
Source:
Biochemical and biophysical research communications 2018 v.506 no.2 pp. 403-408
ISSN:
0006-291X
Subject:
Arabidopsis thaliana; actin; cell growth; cytoplasmic streaming; eukaryotic cells; microfilaments; myosin; organelles; plant development
Abstract:
... Actin is one of the three major cytoskeletal components in eukaryotic cells. Myosin XI is an actin-based motor protein in plant cells. Organelles are attached to myosin XI and translocated along the actin filaments. This dynamic actin–myosin XI system plays a major role in subcellular organelle transport and cytoplasmic streaming. Previous studies have revealed that myosin-driven transport and the ...
DOI:
10.1016/j.bbrc.2017.12.169

13. Activation of human aortic valve interstitial cells by local stiffness involves YAP-dependent transcriptional signaling

Author:
Rosaria Santoro; Denis Scaini; Luisa Ulloa Severino; Francesco Amadeo; Silvia Ferrari; Giacomo Bernava; Gloria Garoffolo; Marco Agrifoglio; Loredana Casalis; Maurizio Pesce
Source:
Biomaterials 2018 v.181 pp. 268-279
ISSN:
0142-9612
Subject:
actin; atomic force microscopy; compliance; cytoskeleton; gels; humans; inflammation; patients; transcription (genetics); transcription factors
Abstract:
... Differentiation of valve interstitial cells (VICs) into pro-calcific cells is one of the central events in calcific aortic valve (AoV) disease (CAVD). While the paracrine pathways and the responsivity of VICs to mechanical compliance of the surrounding environment are well characterized, the molecular programming related to variations in local stiffness, and its link to cytoskeleton dynamics, is l ...
DOI:
10.1016/j.biomaterials.2018.07.033

14. Activation of β- and α2-adrenergic receptors stimulate tubulin polymerization and promote the association of Gβγ with microtubules in cultured NIH3T3 cells

Author:
Jorge A. Sierra-Fonseca; Christina Bracamontes; Jessica Saldecke; Siddhartha Das; Sukla Roychowdhury
Source:
Biochemical and biophysical research communications 2018 v.503 no.1 pp. 102-108
ISSN:
0006-291X
Subject:
actin; agonists; alpha-2 adrenergic receptors; cell division; cell movement; cyclic AMP; isoproterenols; microtubules; neurons; physiological transport; polymerization; signal transduction; tubulin; United Kingdom
Abstract:
... Microtubules (MTs) constitute a crucial part of the cytoskeleton and are essential for cell division and differentiation, cell motility, intracellular transport, and cell morphology. Precise regulation of MT assembly and dynamics is essential for the performance of these functions. Although much progress has been made in identifying and characterizing the cellular factors that regulate MT assembly ...
DOI:
10.1016/j.bbrc.2018.05.188
PubMed:
29852176
PubMed Central:
PMC6070407

15. Active Components from Sea Buckthorn (Hippophae rhamnoides L.) Regulate Hepatic Stellate Cell Activation and Liver Fibrogenesis

Author:
Guokun Zhang; Yifei Liu; Ping Liu
Source:
Journal of agricultural and food chemistry 2018 v.66 no.46 pp. 12257-12264
ISSN:
1520-5118
Subject:
DNA damage; DNA repair; Hippophae rhamnoides; actin; apoptosis; bile ducts; dose response; fibrosis; gene expression regulation; genes; inflammation; liver; liver function; muscles; nutritive value; rats; signal transduction; small fruits; transforming growth factor beta
Abstract:
... Sea buckthorn (Hippophae rhamnoides L.) is a berry bearing multiple nutritional properties. In this study, 46 compounds were isolated from sea buckthorn berries. Preliminary data showed that the components, C13, C15, and C32, exhibited profound inhibitory effect on the activation of hepatic stellate cells (HSCs) induced by transforming growth factor-β (TGF-β) and decreased the levels of inflammato ...
DOI:
10.1021/acs.jafc.8b05306

16. Addition of urea and thiourea to electrophoresis sample buffer improves efficiency of protein extraction from TCA/acetone‐treated smooth muscle tissues for phos‐tag SDS‐PAGE

Author:
Kosuke Takeya; Toshiyuki Kaneko; Motoi Miyazu; Akira Takai
Source:
Electrophoresis 2018 v.39 no.2 pp. 326-333
ISSN:
0173-0835
Subject:
acetone; actin; detection limit; lithium; muscles; myosin; phosphorylation; polyacrylamide gel electrophoresis; protein content; smooth muscle; sulfates; thiourea; trichloroacetic acid; urea
Abstract:
... Phosphorylation analysis by using phos‐tag technique has been reported to be suitable for highly sensitive quantification of smooth muscle myosin regulatory light chain (LC₂₀) phosphorylation. However, there is another factor that will affect the sensitivity of phosphorylation analysis, that is, protein extraction. Here, we optimized the conditions for total protein extraction out of trichloroacet ...
DOI:
10.1002/elps.201700394

17. Adiponectin inhibits osteoclastogenesis by suppressing NF-κB and p38 signaling pathways

Author:
Guiping Chen; Leitao Huang; Xia Wu; Xuqiang Liu; Qiang Xu; Fan Li; Min Dai; Bin Zhang
Source:
Biochemical and biophysical research communications 2018 v.503 no.3 pp. 2075-2082
ISSN:
0006-291X
Subject:
actin; adiponectin; bone density; ligands; osteoclasts; osteoporosis; resorption; signal transduction; transcription factor NF-kappa B
Abstract:
... Adiponectin (APN) has been shown to play a key role in regulating bone mineral density (BMD). Nevertheless, the effects of APN on receptor activator of NF-κB ligand (RANKL)-induced osteoclast formation and mechanism of regulation are not entirely clear. The study, therefore, aimed to evaluate the effect of APN on osteoclastogenesis. Our results showed that APN inhibits osteoclastogenesis and resor ...
DOI:
10.1016/j.bbrc.2018.07.162

18. Agrobacterium-mediated gene delivery and transient expression in the red macroalga Chondrus crispus

Author:
Anusha Devi Ramessur; John H. Bothwell; Christine A. Maggs; Sook Yee Gan; Siew Moi Phang
Source:
Botanica marina 2018 v.61 no.5 pp. 499-510
ISSN:
1437-4323
Subject:
Chlorophyta; Chondrus crispus; acetosyringone; actin; bacteria; beta-glucuronidase; coculture; gene transfer; genetically modified organisms; macroalgae; reporter genes; scanning electron microscopy; staining
Abstract:
... Molecular resources and transgenic studies in red algae are lagging behind those for green algae. The Agrobacterium-mediated gene-transfer method routinely used in plant transformation has not been fully utilised in the red algae, which, as an important source of phycocolloids, warrant more studies. In this regard, a stepwise methodology was developed for Agrobacterium-mediated transformation of t ...
DOI:
10.1515/bot-2018-0028

19. Alogliptin abates memory injuries of hepatic encephalopathy induced by acute paracetamol intoxication via switching-off autophagy-related apoptosis

Author:
Muhammed A. Saad; Alyasaa A. Rastanawi; Muhammed F. El-Yamany
Source:
Life sciences 2018 v.215 pp. 11-21
ISSN:
0024-3205
Subject:
acetaminophen; acetylcholine; acetylcholinesterase; actin; alanine transaminase; alkaline phosphatase; ammonia; apoptosis; aspartate transaminase; autophagy; bioavailability; blood; caspase-3; cytochrome c; dopamine; encephalopathy; enzyme activity; gamma-aminobutyric acid; glutamic acid; hepatocytes; hepatotoxicity; hippocampus; histopathology; inflammation; ligands; liver; memory; memory disorders; muscles; neurotransmitters; norepinephrine; poisoning; rats; serotonin; transforming growth factor beta 1; tumor necrosis factor-alpha
Abstract:
... Hepatic encephalopathy (HE) is a complex neuropsychiatric syndrome. Paracetamol (APAP) causes, in high doses, a hepatic injury. Alogliptin (ALO), with its 100% oral bioavailability, may be able to reverse the acute hepatic injury and memory impairments.Forty rats were divided into four groups as follows; Normal Control Group, APAP intoxicated group, ALO and SIL groups. Behavioral tests (Morris wat ...
DOI:
10.1016/j.lfs.2018.10.069

20. Alterations in the distribution of actin and its binding proteins in the porcine endometrium during early pregnancy: Possible role in epithelial remodeling and embryo adhesion

Author:
Beenu Moza Jalali; Pawel Likszo; Aneta Andronowska; Dariusz J. Skarzynski
Source:
Theriogenology 2018 v.116 pp. 17-27
ISSN:
0093-691X
Subject:
actin; adhesion; binding proteins; conceptus; crosslinking; cytoskeleton; endometrium; epithelial cells; epithelium; estrous cycle; gene expression; integrins; non-specific protein-tyrosine kinase; polymerization; pregnancy; staining; swine; trophoblast
Abstract:
... During early pregnancy, uterine epithelial cells undergo major transformations in their cytoskeleton that make the endometrium receptive for conceptus attachment. Actin binding proteins (ABPs) such as cofilin, gelsolin, and vinculin are involved in regulating actin polymerization, severing or crosslinking actin to integrins. However, whether ABPs are involved in epithelial remodeling or embryo adh ...
DOI:
10.1016/j.theriogenology.2018.05.004