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- Jong, Yuh-Jyh, et al. Show all 4 Authors
- Analytical chemistry 2018 v.90 no.19 pp. 11599-11606
- DNA; diagnostic techniques; fluorescence; fluorescent labeling; genes; genotype; genotyping; magnetism; medicine; muscular atrophy; nucleotides; patients; polymerase chain reaction; restriction endonucleases; single nucleotide polymorphism; streptavidin
- ... In this study, a fast and simple fluorescent genotyping strategy, streptavidin magnetic beads combined with biotin-coupled PCR and restriction-fragment release, was developed for determination of nucleotide variants. This method was further applied for analyzing SMN1 gene in diagnosis of spinal muscular atrophy (SMA). After biotin-coupled PCR, the streptavidin magnetic beads would capture the biot ...