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- Chen, Shuaiyin, et al. Show all 7 Authors
- Biotechnology letters 2018 v.40 no.3 pp. 585-590
- Escherichia coli; Helicobacter pylori; Lactococcus lactis; antiserum; blood serum; digestion; edible vaccines; gastrointestinal system; genes; immune response; immunoglobulin G; mice; mucosal immunity; oral administration; plasmids; polymerase chain reaction; vaccination
- ... OBJECTIVE: To develop a safe and effective oral vaccine against Helicobacter pylori using its HpaA protein expressed in Lactococcus lactis. RESULTS: The gene encoding HpaA was obtained by PCR and ligated to pNZ8110-lysM following digestion with NaeI + SphI. The recombinant plasmid was transferred into E. coli for multiplication, and then into L. lactis. The recombinant L. lactis was induced to exp ...
- Chen, Shuaiyin, et al. Show all 10 Authors
- Journal of chemical technology and biotechnology 2018 v.93 no.6 pp. 1655-1660
- antibodies; antigens; blood serum; edible vaccines; heterologous gene expression; mice; oral vaccination; protein synthesis; proteins; secretion; vaccine development; vector vaccines
- ... BACKGROUND: Food‐grade bacterial expression systems are relatively rare, and increasing evidence indicates that subcellular location of antigens in bacterial vector vaccines may markedly affect the immune efficacy. RESULTS: This study developed a novel food‐grade secretory expression system for heterologous protein production and oral vaccine delivery. Furthermore, by using the expression system, ...