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- Tanihara, F, et al. Show all 8 Authors
- Reproduction in domestic animals 2018 v.53 no.2 pp. 313-318
- apoptosis; blastocyst; electric power; electroporation; exons; genetic engineering; in vitro fertilization; messenger RNA; swine; viability; zygote
- ... This study was conducted to determine suitable conditions for an experimental method in which the CRISPR/Cas9 system is introduced into in vitro‐produced porcine zygotes by electroporation. In the first experiment, when putative zygotes derived from in vitro fertilization (IVF) were electroporated by either unipolar or bipolar pulses, keeping the voltage, pulse duration and pulse number fixed at 3 ...