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1. CgTI, a novel thermostable Kunitz trypsin-inhibitor purified from Cassia grandis seeds: Purification, characterization and termiticidal activity

Author:
Brandão-Costa, Romero M.P.; Araújo, Vivianne Ferreira; Porto, Ana Lucia F.
Source:
International journal of biological macromolecules 2018
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; thermal stability; polyacrylamide gel electrophoresis; isoelectric point; gel chromatography; lethal concentration 50; purification methods; acetone; polypeptides; Nasutitermes; Cassia grandis; Aedes aegypti; molecular weight; seeds; amino acid sequences; toxicity; larvae; temperature; mortality; Artemia; pH;     Show all 21 Subjects
Abstract:
... Cassia grandis trypsin inhibitor (CgTI) is a novel plant serine proteinase inhibitor. This study sets out to purify a thermostable inhibitor from the seeds of Cassia grandis and to provide biochemical information about a novel peptide belonging to the Kunitz family. Moreover, toxicity assays against Artemia, Aedes aegypti larvae-L4 and Nasutitermes corniger are evaluated. The purification process ...
DOI:
10.1016/j.ijbiomac.2018.07.110

2. Comparative transcriptome analysis provides insights of anti‐insect molecular mechanism of Cassia obtusifolia trypsin inhibitor against Pieris rapae

Author:
Xiang, Mian; Zhang, Xian; Deng, Yin; Li, Yangyang; Yu, Jihua; Zhu, Jianquan; Huang, Xinhe; Zhou, Jiayu; Liao, Hai
Source:
Archives of insect biochemistry and physiology 2018 v.97 no.1
ISSN:
0739-4462
Subject:
trypsin inhibitors,   etc   ; Brassicaceae; Pieris rapae; Senna obtusifolia; databases; digestion; gene expression; homeostasis; host plants; ingestion; integrins; larvae; metabolism; midgut; pests; polymerase chain reaction; proteinases; proteolysis; secondary metabolites; signal transduction; transcription (genetics); transcriptomics; unigenes;     Show all 23 Subjects
Abstract:
... Pieris rapae, a serious Lepidoptera pest of cultivated crucifers, utilizes midgut enzymes to digest food and detoxify secondary metabolites from host plants. A recombinant trypsin inhibitor (COTI) from nonhost plant, Cassia obtusifolia, significantly decreased activities of trypsin‐like proteases in the larval midgut on Pieris rapae and could suppress the growth of larvae. In order to know how COT ...
DOI:
10.1002/arch.21427

3. Inactivation Methods of Trypsin Inhibitor in Legumes: A Review

Author:
Avilés‐Gaxiola, Sara; Chuck‐Hernández, Cristina; Serna Saldívar, Sergio O.
Source:
Journal of food science 2018 v.83 no.1 pp. 17-29
ISSN:
0022-1147
Subject:
trypsin inhibitors,   etc   ; absorption; antinutritional factors; arable soils; dietary protein; digestion; energy; functional properties; heat treatment; legumes; metabolites; nutrients; nutritive value; pests; researchers;     Show all 15 Subjects
Abstract:
... Seed legumes have played a major role as a crop worldwide, being cultivated on about 12% to 15% of Earth's arable land; nevertheless, their use is limited by, among other things, the presence of several antinutritional factors (ANFs ‐ naturally occurring metabolites that the plant produces to protect itself from pest attacks.) Trypsin inhibitors (TIs) are one of the most relevant ANFs because they ...
DOI:
10.1111/1750-3841.13985

4. Activity of defense related enzymes and gene expression in pigeon pea (Cajanus cajan) due to feeding of Helicoverpa armigera larvae

Author:
Meitei, Aheibam Loyanganba; Bhattacharjee, Mamta; Dhar, Santanu; Chowdhury, Naimisha; Sharma, Richa; Acharjee, Sumita; Sarmah, Bidyut Kumar
Source:
Journal of plant interactions 2018 v.13 no.1 pp. 231-238
ISSN:
1742-9153
Subject:
trypsin inhibitors,   etc   ; Cajanus cajan; Helicoverpa armigera; cultivars; enzymes; gene expression; gene expression regulation; genes; hydrogen peroxide; larvae; pathogenesis-related proteins; pigeon peas; seed storage;     Show all 13 Subjects
Abstract:
... The biochemical and molecular basis of the defense in a mild tolerant (ICPL-332) and susceptible (ICPL-87) cultivars of pigeon pea (Cajanus cajan) due to Helicoverpa. armigera infestation was studied. We found that feeding by the larvae generated H₂O₂ in a localized manner and activity was observed upto 12 h (hrs) of with a sharp decline within 24 h. Similarly, PPO activity was also detected till ...
DOI:
10.1080/17429145.2018.1466373

5. Improvement of some quality attributes of soymilk through optimization of selected soybean sprouting parameters using response surface methodology

Author:
Oyedeji, Ajibola B.; Mellem, John J.; Ijabadeniyi, Oluwatosin A.
Source:
CyTA: journal of food 2018 v.16 no.1 pp. 230-237
ISSN:
1947-6345
Subject:
trypsin inhibitors,   etc   ; amino acids; color; germination; models; phenolic compounds; phytic acid; regression analysis; response surface methodology; rheological properties; soaking; soybeans; soymilk; sprouting;     Show all 14 Subjects
Abstract:
... This study aimed at producing functional soymilk by optimizing the sprouting conditions of soybeans using response surface methodology. Soaking (12–24 h) and germination times (48–96 h) were optimized using central complete randomized design. Responses obtained from experimental runs were fitted into second order polynomial regression model. Significance of model parameters was tested using ANOVA ...
DOI:
10.1080/19476337.2017.1388292

6. Nutrients and natural toxic substances in commonly consumed Jerusalem artichoke (Helianthus tuberosus L.) tuber

Author:
Judprasong, Kunchit; Archeepsudcharit, Nidthida; Chantapiriyapoon, Kedsiri; Tanaviyutpakdee, Pharrunrat; Temviriyanukul, Piya
Source:
Food chemistry 2018 v.238 pp. 173-179
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; Helianthus tuberosus; Jerusalem artichokes; cadmium; chemical pollutants; cyanides; dietary fiber; freeze drying; fructans; heavy metals; insecticide residues; iron; lead; nitrates; nitrites; nutrient databanks; nutrients; oxalates; phytic acid; potassium; toxic substances; tubers; Thailand;     Show all 23 Subjects
Abstract:
... This study determined nutrients, chemical contaminants, (insecticide residues and heavy metals), and natural toxic substances (nitrate, nitrite, cyanide, oxalate, phytate, and trypsin inhibitor) in tubers of Jerusalem artichokes—Kaentawan in the Thai language—grown in four major provinces in Thailand. They were purchased, prepared, homogenized, and freeze-dried for further analysis using standard ...
DOI:
10.1016/j.foodchem.2016.09.116

7. Protease treatment of canola meal-containing Japanese quail diets: Effect on physiological parameters and meat quality traits

Author:
Mnisi, Caven Mguvane; Mlambo, Victor
Source:
Journal of applied animal research 2018 v.46 no.1 pp. 1389-1394
ISSN:
0974-1844
Subject:
trypsin inhibitors,   etc   ; Coturnix japonica; adults; body weight; canola; canola meal; carcass characteristics; digestion; feed conversion; feed intake; females; growth performance; heat stability; ingredients; mash; meat quality; poultry feeding; protein sources; proteinases; quails; soybeans; weight gain;     Show all 22 Subjects
Abstract:
... The utility of canola meal as protein source for quails may be limited by trypsin inhibitors that interfere with protein digestion. This study was, therefore, designed to assess the effect of including a heat-stable protease mono-enzyme (75’000 PROT/g; EC/IUB no. 3.4.21) in canola meal-containing diets on physiological and meat quality traits of adult female Japanese quails. A total of 240, five-w ...
DOI:
10.1080/09712119.2018.1516670

8. Comparison of Conventional and Microwave Treatment on Soymilk for Inactivation of Trypsin Inhibitors and In Vitro Protein Digestibility

Author:
Vagadia, Brinda Harish; Vanga, Sai Kranthi; Singh, Ashutosh; Gariepy, Yvan; Raghavan, Vijaya
Source:
Foods 2018 v.7 no.1
ISSN:
2304-8158
Subject:
trypsin inhibitors,   etc   ; digestible protein; temperature; processing time; lipoxygenases; soybeans; microwave treatment; cholesterol; production costs; milk; response surface methodology; digestibility; soymilk;     Show all 13 Subjects
Abstract:
... Soymilk is lower in calories compared to cow’s milk, since it is derived from a plant source (no cholesterol) and is an excellent source of protein. Despite the beneficial factors, soymilk is considered as one of the most controversial foods in the world. It contains serine protease inhibitors which lower its nutritional value and digestibility. Processing techniques for the elimination of trypsin ...
DOI:
10.3390/foods7010006

9. Protective effects of soybean protein and egg white protein on the antibacterial activity of nisin in the presence of trypsin

Author:
Pan, Dan; Zhang, Dong; Hao, Limin; Lin, Songyang; Kang, Qiaozhen; Liu, Xin; Lu, Laizheng; Lu, Jike
Source:
Food chemistry 2018 v.239 pp. 196-200
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; Listeria monocytogenes; Staphylococcus aureus; antibacterial properties; cooked foods; egg albumen; food pathogens; meat processing; meat products; nisin; plate count; protective effect; soy protein; total volatile basic nitrogen; trypsin;     Show all 15 Subjects
Abstract:
... The using of nisin to prevent foodborne pathogens (Staphylococcus aureus and Listeria monocytogenes) from contamination has received broad attentions during meat processing. However, the application of nisin has been limited because its antibacterial activity may be inhibited by trypsin. In this study, the protective effects of soybean protein and egg white protein on antibacterial activity of nis ...
DOI:
10.1016/j.foodchem.2017.06.091

10. Unravelling proteome changes of chicken egg whites under carbon dioxide modified atmosphere packaging

Author:
Xu, Lei; Jia, Fei; Luo, Changyao; Yu, Qianqian; Dai, Ruitong; Li, Xingmin
Source:
Food chemistry 2018 v.239 pp. 657-663
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; apolipoprotein D; beta-N-acetylhexosaminidase; carbon; carbon dioxide; chicken eggs; correlation; egg albumen; egg shell; eggs; freshness; humidity; proteome; vitelline membrane;     Show all 14 Subjects
Abstract:
... Unfertilized chicken eggs within 24h of laying were chosen and stored at 25°C and 45% humidity for 0, 20, and 40days. The experimental group (EG) was the carbon dioxide-modified atmosphere packaging (CDMAP) group, whereas the control group (CG) contained eggs without special handling. Egg freshness indexes were measured. The proteome of the egg whites was determined by LC-MS/MS using isobaric tags ...
DOI:
10.1016/j.foodchem.2017.06.128

11. In Vitro Digestibility of Rapeseed and Bovine Whey Protein Mixtures

Author:
Joehnke, Marcel Skejovic; Rehder, Alina; Sørensen, Susanne; Bjergegaard, Charlotte; Sørensen, Jens Christian; Markedal, Keld Ejdrup
Source:
Journal of agricultural and food chemistry 2018 v.66 no.3 pp. 711-719
ISSN:
1520-5118
Subject:
trypsin inhibitors,   etc   ; Brassica napus; beta-lactoglobulin; cattle; digestibility; digestible protein; dithiothreitol; food industry; hydrolysis; lactalbumin; pancreatin; pepsin; plant proteins; protein concentrates; protein sources; rapeseed; whey protein;     Show all 17 Subjects
Abstract:
... Partial replacement of animal protein sources with plant proteins is highly relevant for the food industry, but potential effects on protein digestibility need to be established. In this study, the in vitro protein digestibility (IVPD) of four protein sources and their mixtures (50:50 w/w ratio) was investigated using a transient pepsin hydrolysis (1 h) followed by pancreatin (1 h). The protein so ...
DOI:
10.1021/acs.jafc.7b04681

12. An immunocapture-LC-MS-based assay for serum SPINK1 allows simultaneous quantification and detection of SPINK1 variants

Author:
Ravela, Suvi; Valmu, Leena; Domanskyy, Mykola; Koistinen, Hannu; Kylänpää, Leena; Lindström, Outi; Stenman, Jakob; Hämäläinen, Esa; Stenman, Ulf-Håkan; Itkonen, Outi
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.6 pp. 1679-1688
ISSN:
1618-2642
Subject:
trypsin inhibitors,   etc   ; DNA; amino acids; blood serum; genes; heterozygosity; homozygosity; mutation; pancreas; pancreatitis; patients; renal cell carcinoma; tissues;     Show all 13 Subjects
Abstract:
... Pancreatic secretory trypsin inhibitor Kazal type 1 (SPINK1) is a 6420 Da peptide produced by the pancreas, but also by several other tissues and many tumors. Some mutations of the SPINK1 gene, like the one causing amino acid change N34S, have been shown to confer susceptibility to recurrent or chronic pancreatitis. Detection of such variants are therefore of clinical utility. So far SPINK1 varian ...
DOI:
10.1007/s00216-017-0803-y

13. Solar UV‐B radiation and ethylene play a key role in modulating effective defenses against Anticarsia gemmatalis larvae in field‐grown soybean

Author:
Dillon, Francisco M.; Tejedor, M. Daniela; Ilina, Natalia; Chludil, Hugo D.; Mithöfer, Axel; Pagano, Eduardo A.; Zavala, Jorge A.
Source:
Plant, cell and environment 2018 v.41 no.2 pp. 383-394
ISSN:
0140-7791
Subject:
trypsin inhibitors,   etc   ; Anticarsia gemmatalis; correlation; cultivars; ethylene; flavonols; gene expression; genistin; jasmonic acid; larvae; leaves; messenger RNA; pathogenesis; phytophagous insects; salicylic acid; soybeans; trypsin; ultraviolet radiation;     Show all 18 Subjects
Abstract:
... Solar UV‐B radiation has been reported to enhance plant defenses against herbivore insects in many species. However, the mechanism and traits involved in the UV‐B mediated increment of plant resistance are unknown in crops species, such as soybean. Here, we studied defense‐related responses in undamaged and Anticarsia gemmatalis larvae‐damaged leaves of two soybean cultivars grown under attenuated ...
DOI:
10.1111/pce.13104

14. Two trypsin isoforms from albacore tuna (Thunnus alalunga) liver: Purification and physicochemical and biochemical characterization

Author:
Klomklao, Sappasith; Benjakul, Soottawat
Source:
International journal of biological macromolecules 2018 v.107 pp. 1864-1870
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; Thunnus alalunga; calcium; gel chromatography; liver; molecular weight; pH; polyacrylamide gel electrophoresis; sodium chloride; soybeans; temperature; trypsin; tuna;     Show all 13 Subjects
Abstract:
... Two trypsins (A and B) from the liver of albacore tuna (Thunnus alalunga) were purified to homogeneity using a series of column chromatographies including Sephacryl S-200, Sephadex G-50 and Diethylaminoethyl-cellulose. Purity was increased to 80.35- and 101.23-fold with approximately 3.1 and 19.2% yield for trypsins A and B, respectively. The molecular weights of trypsins A and B were estimated to ...
DOI:
10.1016/j.ijbiomac.2017.10.059

15. Fermentation and enzymatic treatment of pea for turkey nutrition

Author:
Goodarzi Boroojeni, F.; Kozłowski, K.; Jankowski, J.; Senz, M.; Wiśniewska, M.; Boros, D.; Drażbo, A.; Zentek, J.
Source:
Animal feed science and technology 2018 v.237 pp. 78-88
ISSN:
0377-8401
Subject:
trypsin inhibitors,   etc   ; Bacillus licheniformis; Bacillus subtilis; Pisum sativum; alpha-galactosidase; dermatitis; digestibility; dough; enzymatic treatment; experimental diets; fermentation; growth performance; ileum; mash; minerals; nutrients; nutritive value; peas; phytic acid; protein sources; proteinases; resistant starch; spores; turkeys; vitamins;     Show all 25 Subjects
Abstract:
... The present study was conducted to investigate how fermentation (FE) and enzymatic treatment (ET) of pea affect standardized ileal digestibility (SID) of nutrients in diets with peas as the only protein source, and evaluate the consequences of inclusion of different pea products in turkey diets (100 g/kg) on growth performance and bird health. For FE process, Pisum sativum L. was mixed with water ...
DOI:
10.1016/j.anifeedsci.2018.01.008

16. Physico-chemical properties, phytochemicals and DPPH radical scavenging activity of supercritical fluid extruded lentils

Author:
Lv, Ying; Glahn, Raymond P.; Hebb, Richard Lebaron; Rizvi, Syed S.H.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.89 pp. 315-321
ISSN:
0023-6438
Subject:
trypsin inhibitors,   etc   ; 2,2-diphenyl-1-picrylhydrazyl; ambient temperature; antioxidant activity; antioxidants; brittleness; carbon dioxide; extrusion; flour; gelatinization; hardness; lentils; phytopharmaceuticals; porosity; potato starch; rehydration; retrogradation; starch; water solubility; whole grain foods;     Show all 20 Subjects
Abstract:
... A powder blend comprised of lentil flour and pre-gelatinized potato starch was processed using supercritical fluid (carbon dioxide) extrusion (SCFX). The blend was extruded at temperature of 95–99.5 °C and screw speeds of 40, 80 and 120 rpm. The physical properties of the extruded products such as porosity, rehydration rate, expansion ratio, and crispness—improved as the screw speed increased. Fur ...
DOI:
10.1016/j.lwt.2017.10.063

17. Anti-nutrient analysis of 30 Bambara groundnut (Vigna subterranea) accessions in South Africa

Author:
Unigwe, Amara Evangeline; Doria, Enrico; Adebola, Patrick; Gerrano, Abe. S; Pillay, Michael
Source:
Journal of crop improvement 2018 v.32 no.2 pp. 208-224
ISSN:
1542-7536
Subject:
trypsin inhibitors,   etc   ; Vigna subterranea; antinutritional factors; drought tolerance; food security; gallic acid; landraces; nutritive value; phosphates; phytic acid; proanthocyanidins; seeds; supply chain; South Africa; Western Africa;     Show all 15 Subjects
Abstract:
... Bambara groundnut is a leguminous crop that originated in West Africa. It is a drought-tolerant crop and has the potential to provide improved food and nutritional security in the dry areas of Africa. However, the plant contains anti-nutritional factors that affect its food value chain. The objective of this study was to assess the content of four major anti-nutritional factors in 30 bambara groun ...
DOI:
10.1080/15427528.2017.1405857

18. Effects of fermentation on proximate composition, mineral profile and antinutrients of tamarind (Tamarindus indica L.) seed in the production of daddawa-type condiment

Author:
Olagunju, Omotola F.; Ezekiel, Olufunke O.; Ogunshe, Adenike O.; Oyeyinka, Samson A.; Ijabadeniyi, Oluwatosin A.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.90 pp. 455-459
ISSN:
0023-6438
Subject:
trypsin inhibitors,   etc   ; Bacillus licheniformis; Bacillus pumilus; Bacillus subtilis; Tamarindus indica; antinutritional factors; carbohydrates; condiments; consumer acceptance; fermentation; legumes; minerals; nutrients; phosphorus; physicochemical properties; phytic acid; proximate composition; tamarinds;     Show all 18 Subjects
Abstract:
... Tamarind seed is an underutilized legume which can potentially be used as a food condiment. In this study, tamarind seed was fermented using the conventional traditional method of iru (daddawa) production. Samples withdrawn at intervals of 24 h were tested for proximate composition, antinutrients, mineral profile, and other physicochemical properties using standard methods. Protein, fat and carboh ...
DOI:
10.1016/j.lwt.2017.12.064

19. Intraspecific differences in plant defense induction by fall armyworm strains

Author:
Acevedo, Flor E.; Peiffer, Michelle; Ray, Swayamjit; Meagher, Robert; Luthe, Dawn S.; Felton, Gary W.
Source:
Thenew phytologist 2018 v.218 no.1 pp. 310-321
ISSN:
0028-646X
Subject:
trypsin inhibitors,   etc   ; Cynodon dactylon; Spodoptera frugiperda; corn; correlation; elicitors; host plants; insect larvae; intraspecific variation; pathogenesis-related proteins; phospholipase C; phytophagous insects; rice; saliva; secretion;     Show all 15 Subjects
Abstract:
... The underlying adaptive mechanisms by which insect strains are associated with specific plants are largely unknown. In this study, we investigated the role of herbivore‐induced defenses in the host plant association of fall armyworm (Spodoptera frugiperda) strains. We tested the expression of herbivore‐induced defense‐related genes and the activity of plant‐defensive proteins in maize and Bermuda ...
DOI:
10.1111/nph.14981
CHORUS:
10.1111/nph.14981

20. Reductions of anti-nutritional factors of germinated soybeans by ultraviolet and infrared treatments for snack chips preparation

Author:
Maetens, Emma; Hettiarachchy, Navam; Dewettinck, Koen; Horax, Ronny; Moens, Kim
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2018 v.90 pp. 513-518
ISSN:
0023-6438
Subject:
trypsin inhibitors,   etc   ; antinutritional factors; chemical analysis; consumer acceptance; exposure duration; flavor; glycemic index; sensory evaluation; soybeans; starch; texture; ultraviolet radiation;     Show all 12 Subjects
Abstract:
... Ultraviolet (UV) and infrared (IR) treatments were conducted to reduce anti-nutritional factors of 5-day germinated soybean. Exposure time/duration and distance/height to UV/IR were 24/48/72 h for UV treatment at 7/17/27 cm distance and 1.0/1.5/2.0 h for IR treatment at 15/20/25 cm distance. These resulted in significant reductions (P < .05) of lipoxygenase-1, lipoxygenase-3, and trypsin inhibitor ...
DOI:
10.1016/j.lwt.2018.01.001