Publication year rev: 7983-2017 / Publication Year: 2017 / Subject term: cytoskeleton

1. 2,3,7,8-Tetrachlorodibenzo-p-dioxin influences bovine herpesvirus 1 replication through upregulation of SIRT3 and cytoskeletal reorganization

Author:: Filomena Fiorito; Valentina Iovane; Annarosaria Marullo; Anna Costagliola; Giovanna Elvira Granato; Luisa De Martino
Source:: Veterinary research communications 2017 v.41 no.4 pp. 299-306
ISSN:: 0165-7380
Subject:: proteins; Bovine alphaherpesvirus 1; kidney cells; tetrachlorodibenzo-p-dioxin; cytoskeleton; veterinary medicine; viruses; mitochondria; virus replication; apoptosis
Abstract:: ... Infection of kidney cells (MDBK) with Bovine Herpesvirus 1 (BoHV-1) is affected by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which accelerates BoHV-1-induced apoptosis and increases virus replication. Herein, to elucidate the mechanism through TCDD modifies BoHV-1 infection, we analyzed the modulation of a members of Sirtuin proteins family in MDBK cells. We found that mitochondrial SIRT3 was up ...
DOI:: 10.1007/s11259-017-9701-1
PubMed:: 29081026
PubMed Central:: PMC7088714
: https://doi.org/10.1007/s11259-017-9701-1

2. A Cyclin D2-derived peptide acts on specific cell cycle phases by activating ERK1/2 to cause the death of breast cancer cells

Author:: Lilian C. Russo; Christiane B. Araujo; Leo K. Iwai; Emer S. Ferro; Fabio L. Forti
Source:: Journal of proteomics 2017 v.151 pp. 24-32
ISSN:: 1874-3919
Subject:: affinity chromatography; apoptosis; breast neoplasms; cell proliferation; confocal microscopy; cyclins; cytoskeleton; death; humans; interphase; mass spectrometry; medicinal properties; mitogen-activated protein kinase; neoplasm cells; peptides; phosphorylation; proteasome endopeptidase complex; protein degradation; rats
Abstract:: ... Protein degradation by the proteasome generates functional intracellular peptides. Pep5, a peptide derived from Cyclin D2, induces cell death in tumor cell lines and reduces the volume of rat C6 glioblastoma tumors in vivo. Here, we chose the human MDA-MB-231 breast cancer cells to evaluate the mechanism of cell death induced by pep5 in different phases of the cell cycle. Fluorescently labeled pep ...
DOI:: 10.1016/j.jprot.2016.06.028
: https://doi.org/10.1016/j.jprot.2016.06.028

3. A Noncanonical Binding Site in the EVH1 Domain of Vasodilator-Stimulated Phosphoprotein Regulates Its Interactions with the Proline Rich Region of Zyxin

Author:: Acevedo Lucila Andrea; Greenwood Alexander I.; Nicholson Linda K.
Source:: Biochemistry 2017 v.56 no.35 pp. 4626-4636
ISSN:: 1520-4995
Subject:: actin; binding sites; cell movement; cytoskeleton; models; mutation; nuclear magnetic resonance spectroscopy; phosphoproteins; phosphorylation; proline; stoichiometry; titration
Abstract:: ... Vasodilator-stimulated phosphoprotein (VASP) is a processive actin polymerase with roles in the control of cell shape and cell migration. Through interaction with the cytoskeletal adaptor protein Zyxin, VASP can localize to damaged stress fibers where it serves to repair and reinforce these structures. VASP localization is mediated by its N-terminal Ena/VASP homology (EVH1) domain, which binds to ...
DOI:: 10.1021/acs.biochem.7b00618
PubMed:: 28783324
PubMed Central:: PMC5724578
: https://doi.org/10.1021/acs.biochem.7b00618

4. A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis

Author:: Thomas M. Bartlett; Benjamin P. Bratton; Amit Duvshani; Amanda Miguel; Ying Sheng; Nicholas R. Martin; Jeffrey P. Nguyen; Alexandre Persat; Samantha M. Desmarais; Michael S. VanNieuwenhze; Kerwyn Casey Huang; Jun Zhu; Joshua W. Shaevitz; Zemer Gitai
Source:: Cell 2017 v.168 pp. 172-185.e15
ISSN:: 0092-8674
Subject:: Vibrio cholerae; cytoskeleton; human health; hydrocolloids; pathogenesis; peptidoglycans; polymers; quantitative analysis
Abstract:: ... Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V. cholerae. CrvA self-assembles into filaments at the inner face of cell curvatur ...
DOI:: 10.1016/j.cell.2016.12.019
PubMed:: 28086090
PubMed Central:: PMC5287421
: https://doi.org/10.1016/j.cell.2016.12.019

5. A Shotgun Proteomics Approach Reveals a New Toxic Role for Alzheimer’s Disease Aβ Peptide: Spliceosome Impairment

Author:: Nuzzo Domenico; Inguglia Luigi; Walters Jessica; Picone Pasquale; Di Carlo Marta
Source:: Journal of Proteome Research 2017 v.16 no.4 pp. 1526-1541
ISSN:: 1535-3907
Subject:: Alzheimer disease; Western blotting; biogenesis; bioinformatics; cytoskeleton; models; neurodegenerative diseases; pathogenesis; proteins; proteome; proteomics; spliceosomes; toxicity
Abstract:: ... Proteomic changes have been described in many neurodegenerative diseases, including Alzheimer’s disease (AD). However, the early events in the onset of the pathology are yet to be fully elucidated. A cell model system in which LAN5 neuroblastoma cells were incubated for a short time with a recombinant form of Aβ₄₂ was utilized. Proteins extracted from these cells were subjected to shotgun proteomi ...
DOI:: 10.1021/acs.jproteome.6b00925
: https://doi.org/10.1021/acs.jproteome.6b00925

6. A genome‐wide association study of heat stress‐associated SNPs in catfish

Author:: Y. Jin; T. Zhou; X. Geng; S. Liu; A. Chen; J. Yao; C. Jiang; S. Tan; B. Su; Z. Liu
Source:: Animal genetics 2017 v.48 no.2 pp. 233-236
ISSN:: 0268-9146
Subject:: Ictalurus furcatus; Ictalurus punctatus; backcrossing; breeding programs; catfish; climate change; cytoskeleton; energy metabolism; females; genes; genome-wide association study; heat; heat stress; heat tolerance; hybrids; interspecific hybridization; iron; linkage groups; males; protein degradation; protein folding; single nucleotide polymorphism; ubiquitination
Abstract:: ... Heat tolerance is a complex and economically important trait for catfish genetic breeding programs. With global climate change, it is becoming an increasingly important trait. To better understand the molecular basis of heat stress, a genome‐wide association study (GWAS) was carried out using the 250 K catfish SNP array with interspecific backcross progenies, which derived from crossing female cha ...
DOI:: 10.1111/age.12482
CHORUS:: 10.1111/age.12482
: https://doi.org/10.1111/age.12482

7. A high-throughput QCM chip configuration for the study of living cells and cell-drug interactions

Author:: Haibo Shen; Tiean Zhou; Jiajin Hu
Source:: Analytical and bioanalytical chemistry 2017 v.409 no.27 pp. 6463-6473
ISSN:: 1618-2642
Subject:: acoustics; cytoskeleton; cytotoxicity; drug evaluation; glass; human umbilical vein endothelial cells; isoprenaline; monitoring; paclitaxel; phenotype; quartz; screening; silicone; verapamil; viscoelasticity; viscosity
Abstract:: ... In this study, we present a novel design of interference-free, negligible installation-induced stress, suitable for the fabrication of high-throughput quartz crystal microbalance (HQCM) chips. This novel HQCM chip configuration was fabricated using eight independent yet same-batch quartz crystal resonators within a common glass substrate with eight through-holes of diameter slightly larger than th ...
DOI:: 10.1007/s00216-017-0591-4
: https://doi.org/10.1007/s00216-017-0591-4

8. A novel ex vivo immunoproteomic approach characterising Fasciola hepatica tegumental antigens identified using immune antibody from resistant sheep

Author:: Timothy C. Cameron; Ira Cooke; Pierre Faou; Hayley Toet; David Piedrafita; Neil Young; Vignesh Rathinasamy; Travis Beddoe; Glenn Anderson; Robert Dempster; Terry W. Spithill
Source:: International journal for parasitology 2017 v.47 no.9 pp. 555-567
ISSN:: 0020-7519
Subject:: Fasciola gigantica; Fasciola hepatica; adaptive immunity; adults; amino acids; antibodies; antigens; cytoskeleton; cytotoxicity; definitive hosts; exocytosis; fascioliasis; flukes; glucose transporters; immune response; immunoglobulin G; immunotherapy; juveniles; lysosomes; mass spectrometry; metacercariae; multiple drug resistance; protein secretion; sequence homology; sheep; vaccines; vacuoles
Abstract:: ... A more thorough understanding of the immunological interactions between Fasciola spp. and their hosts is required if we are to develop new immunotherapies to control fasciolosis. Deeper knowledge of the antigens that are the target of the acquired immune responses of definitive hosts against both Fasciola hepatica and Fasciola gigantica will potentially identify candidate vaccine antigens. Indones ...
DOI:: 10.1016/j.ijpara.2017.02.004
: https://doi.org/10.1016/j.ijpara.2017.02.004

9. A novel microfluidic device to model the human proximal tubule and glomerulus

Author:: Courtney M. Sakolish; Gretchen J. Mahler
Source:: RSC advances 2017 v.7 no.8 pp. 4216-4225
ISSN:: 2046-2069
Subject:: blood proteins; cell lines; cytoskeleton; filtration; glomerular filtration rate; humans; kidneys; models; protein synthesis; shear stress
Abstract:: ... We have developed a re-usable multi-layer microfluidic device to model the human kidney that incorporates a porous growth substrate, physiological fluid flow, and the passive filtration of the glomerulus. The target cell line in this project is HK-2, immortalized human kidney proximal tubule cells. Cells were exposed to a shear stress of 0.8 dyne cm⁻² within the device and monitored for protein ex ...
DOI:: 10.1039/c6ra25641d
: https://doi.org/10.1039/c6ra25641d

10. A proteomic approach to assess the host response in gills of farmed Atlantic salmon Salmo salar L. affected by amoebic gill disease

Author:: Mar Marcos-López; Hamish D. Rodger; Ian O'Connor; Mark Braceland; Richard J.S. Burchmore; P. David Eckersall; Eugene MacCarthy
Source:: Aquaculture 2017 v.470 pp. 1-10
ISSN:: 0044-8486
Subject:: Salmo salar; aerobiosis; apolipoprotein A-I; aquaculture; cell cycle; cyclophilins; cytoskeleton; dissociation; ectoparasites; epithelium; gene expression regulation; gills; histones; hyperplasia; immunity; infectious diseases; peptides; protein content; proteomics; screening; smolts; two-dimensional gel electrophoresis
Abstract:: ... Amoebic gill disease (AGD), caused by the marine ectoparasite Neoparamoeba perurans, is one of the most significant infectious diseases for Atlantic salmon (Salmo salar) aquaculture. Upon colonisation of the host gills, the parasite induces a marked hyperplasia and fusion of the lamellar epithelium, which can severely compromise the host if left untreated. In order to investigate the host response ...
DOI:: 10.1016/j.aquaculture.2016.12.009
: https://doi.org/10.1016/j.aquaculture.2016.12.009

11. ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

Author:: Ewa Szypulska; Kamil Jankowski; Stanisław Weidner
Source:: Acta physiologiae plantarum 2017 v.39 no.8 pp. 190
ISSN:: 0137-5881
Subject:: Hordeum vulgare; abscisic acid; actin; barley; biogenesis; biosynthesis; cell walls; cellulose; cytoskeleton; energy; energy metabolism; fruits; glycolysis; hemicellulose; lignification; lignin; messenger RNA; oxidative stress; protein metabolism; proteome; proteomics; salt stress; seedlings; signal transduction; sodium chloride; stress tolerance; translation (genetics)
Abstract:: ... A proteomic study was conducted on caryopses of barley cv. Stratus (Hordeum vulgare). The caryopses were germinated in darkness at 20 °C, according to one of the three experimental setups: (a) in distilled water for 24 h, followed by 100 mM NaCl for another 24 h (salinity stress, SS); (b) in 100 µM abscisic acid for 24 h, with rinsing in distilled water to remove residual ABA, followed by 100 mM N ...
DOI:: 10.1007/s11738-017-2490-x
: https://doi.org/10.1007/s11738-017-2490-x

12. Acoustic tweezing cytometry enhances osteogenesis of human mesenchymal stem cells through cytoskeletal contractility and YAP activation

Author:: Xufeng Xue; Xiaowei Hong; Zida Li; Cheri X. Deng; Jianping Fu
Source:: Biomaterials 2017 v.134 pp. 22-30
ISSN:: 0142-9612
Subject:: acoustics; actin; biophysics; bone diseases; bone formation; cell culture; cellular microenvironment; chemical bonding; cytoskeleton; humans; integrins; lipids; mechanics; medicine; microbubbles; myosin; stem cells; tissue engineering; transcription factors; ultrasonics
Abstract:: ... Human mesenchymal stem cells (hMSCs) have great potential for cell-based therapies for treating degenerative bone diseases. It is known that mechanical cues in the cell microenvironment play an important role in regulating osteogenic (bone) differentiation of hMSCs. However, mechanoregulation of lineage commitment of hMSCs in conventional two-dimensional (2D) monocultures or bioengineered three-di ...
DOI:: 10.1016/j.biomaterials.2017.04.039
PubMed:: 28453955
PubMed Central:: PMC5506541
: https://doi.org/10.1016/j.biomaterials.2017.04.039

13. Activated microglia trigger inflammasome activation and lysosomal destabilization in human RPE cells

Author:: Christopher Nebel; Alexander Aslanidis; Khalid Rashid; Thomas Langmann
Source:: Biochemical and biophysical research communications 2017 pp. -
ISSN:: 0006-291X
Subject:: autophagy; cell culture; cytoskeleton; epithelium; granulocyte-macrophage colony-stimulating factor; humans; interleukin-6; interleukin-8; lipopolysaccharides; macular degeneration; neuroglia; staining
Abstract:: ... Activation of the innate immune system plays a major role in retinal degenerative diseases including age-related macular degeneration (AMD). In this study, we investigated whether reactive microglia trigger and sustain NLRP3 inflammasome activation in human retinal pigment epithelium (ARPE-19) cells. Specifically, we analyzed the potential of cell culture supernatants from lipopolysaccharide (LPS) ...
DOI:: 10.1016/j.bbrc.2017.01.176
: https://doi.org/10.1016/j.bbrc.2017.01.176

14. Adhesion of bone marrow mesenchymal stem cells on porous titanium surfaces with strontium-doped hydroxyapatite coating

Author:: Dan-Li Fu; Qiao-hong Jiang; Fu-ming He; Bai-ping Fu
Source:: Journal of Zhejiang University 2017 v.18 no.9 pp. 778-788
ISSN:: 1673-1581
Subject:: CD29 antigen; actin; bone marrow; bromides; cell adhesion; cell proliferation; cell viability; coatings; cytoskeleton; electrochemistry; fluorescence; gene expression; hydroxyapatite; non-specific protein-tyrosine kinase; quantitative polymerase chain reaction; rats; reverse transcriptase polymerase chain reaction; scanning electron microscopy; staining; stem cells; titanium; transcription (genetics)
Abstract:: ... OBJECTIVE: To determine the adhesion behavior of bone marrow mesenchymal stem cells (MSCs) on a titanium surface with a nanostructured strontium-doped hydroxyapatite (Sr-HA) coating. METHODS: Sr-HA coatings were applied on roughened titanium surfaces using an electrochemical deposition method. Primary cultured rat MSCs were seeded onto Sr-HA-, HA-coated titanium, and roughened titanium surfaces, a ...
DOI:: 10.1631/jzus.B1600517
PubMed Central:: PMC5611549
: https://doi.org/10.1631/jzus.B1600517

15. Adhesion profile and differentiation capacity of human adipose tissue derived mesenchymal stem cells grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coated surfaces

Author:: R. Beklem Bostancioglu; Mevlut Gurbuz; Ayse Gul Akyurekli; Aydin Dogan; A. Savas Koparal; A. Tansu Koparal
Source:: Colloids and surfaces 2017 v.155 pp. 415-428
ISSN:: 0927-7765
Subject:: DNA; actin; adhesion; adipose tissue; air; alizarin; alkaline phosphatase; biocompatibility; bone formation; cell adhesion; cell viability; colloids; copper; culture media; cytoskeleton; extracellular matrix; fluorescent antibody technique; genes; glass; humans; hydroxyapatite; metal ions; microarray technology; scanning electron microscopy; silver; spray deposition; staining; stem cells; zinc
Abstract:: ... Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human adipose tissue derived mesenchymal stem cells (hMSC)', were grown on metal ion (Zn, Ag and Cu) ...
DOI:: 10.1016/j.colsurfb.2017.04.015
: https://doi.org/10.1016/j.colsurfb.2017.04.015

16. Aeromonas salmonicida type III secretion system-effectors-mediated immune suppression in rainbow trout (Oncorhynchus mykiss)

Author:: F.C. Origgi; O. Benedicenti; H. Segner; U. Sattler; T. Wahli; J. Frey
Source:: Fish & shellfish immunology 2017 v.60 pp. 334-345
ISSN:: 1050-4648
Subject:: Aeromonas salmonicida subsp. salmonicida; Oncorhynchus mykiss; aquaculture; bacteria; cytoskeleton; death; fish; furunculosis; immune response; pathogens; phagocytosis; toxins; type III secretion system; virulence
Abstract:: ... Aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen in aquaculture. Together with other pathogens, it is characterized by the presence of a type 3 secretion system (T3SS). The T3SS is the main virulence mechanism of A. salmonicida. It is used by the bacterium to secrete and translocate several toxins and effector proteins into the host cell. Some of t ...
DOI:: 10.1016/j.fsi.2016.12.006
: https://doi.org/10.1016/j.fsi.2016.12.006

17. Aerosol from Tobacco Heating System 2.2 has reduced impact on mouse heart gene expression compared with cigarette smoke

Author:: Justyna Szostak; Stéphanie Boué; Marja Talikka; Emmanuel Guedj; Florian Martin; Blaine Phillips; Nikolai V. Ivanov; Manuel C. Peitsch; Julia Hoeng
Source:: Food and chemical toxicology 2017 v.101 pp. 157-167
ISSN:: 0278-6915
Subject:: actin; aerosols; at-risk population; breathing; cardiovascular diseases; cigarettes; cytoskeleton; filamin; gene expression; gene expression regulation; genes; heart; inflammation; mice; risk; smoke; smoking (habit); tissues; toxicology; transcriptomics
Abstract:: ... Experimental studies clearly demonstrate a causal effect of cigarette smoking on cardiovascular disease. To reduce the individual risk and population harm caused by smoking, alternative products to cigarettes are being developed. We recently reported on an apolipoprotein E-deficient (Apoe−/-) mouse inhalation study that compared the effects of exposure to aerosol from a candidate modified risk tob ...
DOI:: 10.1016/j.fct.2017.01.013
: https://doi.org/10.1016/j.fct.2017.01.013

18. Analysis of differentially accumulated proteins associated with graft union formation in pecan (Carya illinoensis)

Author:: Zhenghai Mo; Haiyang He; Wenchuan Su; Fangren Peng
Source:: Scientia horticulturae 2017 v.224 pp. 126-134
ISSN:: 0304-4238
Subject:: Carya illinoinensis; allografting; amino acids; aminocyclopropanecarboxylate oxidase; ascorbate peroxidase; callus; cytoskeleton; energy metabolism; ethylene production; juveniles; mass spectrometry; pecans; peroxiredoxin; protein metabolism; proteome; reactive oxygen species; ribosomal proteins; tracheary elements; tubulin; two-dimensional gel electrophoresis
Abstract:: ... Grafting is of particular importance for the mass cultivation of pecan (Carya illinoensis), which can greatly shorten the long juvenile phase of this species. To reveal the molecular mechanism underlying graft union formation, the dynamic proteome changes at the graft union were investigated 0, 3, 8, 15, and 30days after grafting in homograft of pecan by two-dimensional gel electrophoresis. Forty- ...
DOI:: 10.1016/j.scienta.2017.06.005
: https://doi.org/10.1016/j.scienta.2017.06.005

19. Analysis of the spleen proteome of chickens infected with reticuloendotheliosis virus

Author:: Mei Xue; Yan Zhao; Shunlei Hu; Xingming Shi; Hongyu Cui; Yunfeng Wang
Source:: Archives of virology 2017 v.162 no.5 pp. 1187-1199
ISSN:: 0304-8608
Subject:: Reticuloendotheliosis virus; antioxidants; cell proliferation; chickens; cytoskeleton; electrospray ionization mass spectrometry; gels; gene expression regulation; immunosuppression; liquid chromatography; molecular chaperones; protein synthesis; proteome; secondary infection; signal transduction; spleen; two-dimensional gel electrophoresis
Abstract:: ... Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the family Retroviridae, can result in immunosuppression and subsequent increased susceptibility to secondary infections. In the present study, we identified differentially expressed proteins in the spleens of chickens infected with the REV-A HLJ07I strain, using two-dimensional gel electrophoresis on samples from time points c ...
DOI:: 10.1007/s00705-016-3180-5
PubMed:: 28097424
PubMed Central:: PMC5387025
: https://doi.org/10.1007/s00705-016-3180-5

20. Andrographolide disrupts meiotic maturation by blocking cytoskeletal reorganisation and decreases the fertilisation potential of mouse oocytes

Author:: Hong-xing Liang; Sheng-sheng Lu; Zheng Yan; Yan-ping Kuang; Xiang-xing Zhu; Zhi-guang Yan; Tong Du; Wei-ran Chai; Hui Long; Qi-feng Lyu
Source:: Reproduction, fertility, and development 2017 v.29 no.12 pp. 2336-2344
ISSN:: 1031-3613
Subject:: Andrographis paniculata; actin; adverse effects; andrographolide; apoptosis; cytokinesis; cytoskeleton; dose response; female fertility; infectious diseases; intracytoplasmic sperm injection; leaves; meiosis; mice; oocytes; Asia
Abstract:: ... Andrographolide (AG) is a diterpenoid lactone isolated from the stem and leaves of Andrographis paniculata Nees that is used for the effective treatment of infectious diseases in Asian countries. Previous studies have reported adverse effects of AG on female fertility in rodents; however, the underlying mechanisms are unknown. The aim of the present study was to investigate the effects of AG on th ...
DOI:: 10.1071/RD16343
: https://doi.org/10.1071/RD16343

21. Anti-inflammatory effects of the selective phosphodiesterase 3 inhibitor, cilostazol, and antioxidants, enzymatically-modified isoquercitrin and α-lipoic acid, reduce dextran sulphate sodium-induced colorectal mucosal injury in mice

Author:: Yumi Kangawa; Toshinori Yoshida; Hajime Abe; Yoshiki Seto; Taishi Miyashita; Michi Nakamura; Tohru Kihara; Shim-mo Hayashi; Makoto Shibutani
Source:: Experimental and toxicologic pathology 2017 v.69 no.4 pp. 179-186
ISSN:: 0940-2993
Subject:: anti-inflammatory activity; antioxidants; apoptosis; blood; body weight changes; cell adhesion; cell proliferation; chemokines; colitis; colon; cytoskeleton; dextran; diarrhea; edema; gene expression; genome; histopathology; interleukins; isoquercitrin; lipoic acid; mice; microarray technology; pathogenesis; platelet activation; sulfates; tumor necrosis factors
Abstract:: ... Developing effective treatments and preventing inflammatory bowel disease (IBD) are urgent challenges in improving patients’ health. It has been suggested that platelet activation and reactive oxidative species generation are involved in the pathogenesis of IBD. We examined the inhibitory effects of a selective phosphodiesterase-3 inhibitor, cilostazol (CZ), and two antioxidants, enzymatically mod ...
DOI:: 10.1016/j.etp.2016.12.004
: https://doi.org/10.1016/j.etp.2016.12.004

22. Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows

Author:: Gagaoua Mohammed; Couvreur Sébastien; Le Bec Guillain; Aminot Ghislain; Picard Brigitte
Source:: Journal of agricultural and food chemistry 2017 v.65 no.17 pp. 3569-3580
ISSN:: 1520-5118
Subject:: Maine-Anjou; beef; biomarkers; color; cows; cytoskeleton; enzymes; equations; heat shock proteins; longissimus muscle; metabolism; models; myofibrillar proteins; pH; protected designation of origin
Abstract:: ... This study investigated the relationships among a list of 23 protein biomarkers with CIE-L*a*b* meat color traits and ultimate pH on Longissimus thoracis (LT) and Rectus abdominis (RA) muscles of 48 protected designation of origin Maine-Anjou cows. The technological parameters were correlated with several biomarkers and were in some cases muscle-dependent. More biomarkers were related to pHu in LT ...
DOI:: 10.1021/acs.jafc.7b00434
: https://doi.org/10.1021/acs.jafc.7b00434

23. Automated three-dimensional reconstruction of the Caenorhabditis elegans germline

Author:: Sandeep Gopal; Peter Boag; Roger Pocock
Source:: Developmental biology 2017 pp. -
ISSN:: 0012-1606
Subject:: Caenorhabditis elegans; apoptosis; chromosomes; cytoskeleton; germ cells; methodology; models; mutation; phenotype; protein synthesis; proteins; stem cells
Abstract:: ... The Caenorhabditis elegans germline is widely used as a model to study stem cell development, chromosome dynamics and apoptosis. Major readouts of germline phenotypes such as cell counting and protein expression profiling are routinely analysed manually and in a two-dimensional manner. The major disadvantages of the existing approaches are 1) they are time-consuming and laborious and 2) there is a ...
DOI:: 10.1016/j.ydbio.2017.10.004
: https://doi.org/10.1016/j.ydbio.2017.10.004

24. Bacteria and endothelial cells: a toxic relationship

Author:: Ashira Lubkin; Victor J Torres
Source:: Current opinion in microbiology 2017 v.35 pp. 58-63
ISSN:: 1369-5274
Subject:: bacteria; blood flow; cytoskeleton; endothelial cells; endothelium; immune response; leukocytes; sepsis (infection); toxicity; toxins
Abstract:: ... Pathogenic bacteria use the bloodstream as a highway for getting around the body, and thus have to find ways to enter and exit through the endothelium. Many bacteria approach this problem by producing toxins that can breach the endothelial barrier through diverse creative mechanisms, including directly killing endothelial cells (ECs), weakening the cytoskeleton within ECs, and breaking the junctio ...
DOI:: 10.1016/j.mib.2016.11.008
PubMed:: 28013162
PubMed Central:: PMC5481510
: https://doi.org/10.1016/j.mib.2016.11.008

25. Betanodavirus-like particles enter host cells via clathrin-mediated endocytosis in a cholesterol-, pH- and cytoskeleton-dependent manner

Author:: Runqing Huang; Guohua Zhu; Jing Zhang; Yuxiong Lai; Yu Xu; Jianguo He; Junfeng Xie
Source:: Veterinary research 2017 v.48 no.1 pp. 8
ISSN:: 1297-9716
Subject:: Betanodavirus; Epinephelus coioides; Lates calcarifer; ammonia; chlorides; chloroquine; chlorpromazine; cholera toxin; cholesterol; clathrin; cytochalasin D; cytoskeleton; endocytosis; farmed fish; financial economics; freshwater; genome; juveniles; larvae; models; necrosis; pH; small interfering RNA; vaccine development; viruses
Abstract:: ... Betanodavirus, also referred to nervous necrosis virus (NNV), is the causative agent of the fatal disease, viral nervous necrosis and has brought significant economic losses in marine and freshwater cultured fish, especially larvae and juveniles. Here, we used an established invasion model with virus-like particle (VLP)-cells, mimicking orange-spotted grouper nervous necrosis virus (OGNNV), to inv ...
DOI:: 10.1186/s13567-017-0412-y
PubMed:: 28179028
PubMed Central:: PMC5299686
: https://doi.org/10.1186/s13567-017-0412-y

26. Bioinformatic search for Ca2+- and calmodulin-dependent protein kinases potentially associated with the regulation of plant cytoskeleton

Author:: D. O. Novozhylov; P. A. Karpov; Ya. B. Blume
Source:: Cytology and genetics 2017 v.51 no.4 pp. 239-246
ISSN:: 0095-4527
Subject:: Arabidopsis thaliana; Homo sapiens; Mus musculus; active sites; animal proteins; animals; bioinformatics; calcium-calmodulin-dependent protein kinase; cytoskeleton; phylogeny
Abstract:: ... The paper presents the results of a bioinformatic search for Ca²⁺- and calmodulin-dependent protein kinases from Arabidopsis thaliana, which may potentially participate in cytoskeleton regulation. Homologues were chosen based on their similarity with the calmodulin-dependent protein kinases from Homo sapiens and Mus musculus, which modulate the structure and dynamic behavior of the cytoskeleton. I ...
DOI:: 10.3103/S0095452717040053
: https://doi.org/10.3103/S0095452717040053

27. Biological Responses and Mechanisms of Human Bone Marrow Mesenchymal Stem Cells to Zn and Mg Biomaterials

Author:: Zhu Donghui; Su Yingchao; Young Marcus L.; Ma Jun; Zheng Yufeng; Tang Liping
Source:: ACS applied materials & interfaces 2017 v.9 no.33 pp. 27453-27461
ISSN:: 1944-8252
Subject:: alizarin; alloys; biocompatibility; biocompatible materials; bone formation; bone marrow; calcium; cell adhesion; cell communication; cell viability; collagen; cytoskeleton; extracellular matrix; genes; humans; magnesium; mechanical properties; mineralization; mitogen-activated protein kinase; osteopontin; quantitative polymerase chain reaction; receptors; staining; stem cells; zinc
Abstract:: ... Zn biomaterials attract strong attentions recently for load-bearing medical implants because of their mechanical properties similar to bone, biocompatibility, and degradability at a more matched rate to tissue healing. It has been shown previously that Zn alloys are beneficial for bone regeneration, but the supporting mechanisms have not been explored in detail. Here, we studied the biological res ...
DOI:: 10.1021/acsami.7b06654
: https://doi.org/10.1021/acsami.7b06654

28. Blastocyst hatching in humans

Author:: R. A. Shafei; A. G. Syrkasheva; A. Yu. Romanov; N. P. Makarova; N. V. Dolgushina; M. L. Semenova
Source:: Russian journal of developmental biology 2017 v.48 no.1 pp. 5-15
ISSN:: 1062-3604
Subject:: animal models; blastocyst; cysteine proteinases; cytoskeleton; desmosomes; endometrium; epithelium; extracellular matrix; glycoproteins; hatching; humans; oocytes; serine proteinases; zona pellucida
Abstract:: ... The human oocyte is surrounded by the zona pellucida—an elastic, transparent extracellular matrix consisting of specific glycoproteins. The zona pellucida is preserved after fertilization and surrounds the developing human embryo for a few days. The embryo needs to get out of the zona pellucida before implantation to establish cell contacts between the trophectoderm and endometrial epithelium. The ...
DOI:: 10.1134/S1062360417010106
: https://doi.org/10.1134/S1062360417010106

29. Blebbistatin induces chondrogenesis of single mesenchymal cells via PI3K/PDK1/mTOR/p70S6K pathway

Author:: Hyoin Kim; Dong Hyun Kim; Bohyeon Jeong; Ju-Hee Kim; Sun-Ryung Lee; Jong Kyung Sonn
Source:: Biológia 2017 v.72 no.6 pp. 694-701
ISSN:: 1336-9563
Subject:: actin; chondrogenesis; mammals; microfilaments; myosin; phosphatidylinositol 3-kinase; phosphorylation; protein kinases; rapamycin; ribosomal proteins; signal transduction
Abstract:: ... Rearrangement of the actin cytoskeleton plays an inductive role in chondrogenic differentiation. Our previous study showed that blebbistatin, an inhibitor of myosin II, removes actin stress fibres and induces chondrogenesis of mesenchymal cells in monolayer cultures. In the present study, we investigated signalling pathways implicated in the induction of chondrogenesis by dissolving actin stress f ...
DOI:: 10.1515/biolog-2017-0078
: https://doi.org/10.1515/biolog-2017-0078

30. Blood-brain barrier dysfunction induced by silica NPs in vitro and in vivo: Involvement of oxidative stress and Rho-kinase/JNK signaling pathways

Author:: Xin Liu; Baiyan Sui; Jiao Sun
Source:: Biomaterials 2017 v.121 pp. 64-82
ISSN:: 0142-9612
Subject:: adverse effects; astrocytes; blood-brain barrier; brain; circulatory system; cytoskeleton; endothelial cells; humans; in vivo studies; inflammation; models; nanoparticles; neurotoxicity; oxidative stress; rats; reactive oxygen species; signal transduction; silica; tight junctions; vascular endothelial growth factors
Abstract:: ... Silica nanoparticles (SiO2-NPs) has been extensively exploited in biomedical fields and mostly designed to enter the circulatory system, however, few studies focused on the potential adverse effects of SiO2-NPs exposure on the blood-brain barrier (BBB) that serves as a critical barrier between the central nervous system (CNS) and the peripheral circulation. This study attempts to provide an unders ...
DOI:: 10.1016/j.biomaterials.2017.01.006
: https://doi.org/10.1016/j.biomaterials.2017.01.006

31. Brain proteomic modifications associated to protective effect of grape extract in a murine model of obesity

Author:: Selima Smine; Antoine Obry; Safwen Kadri; Julie Hardouin; Manuel Fréret; Mohamed Amri; Thierry Jouenne; Ferid Limam; Pascal Cosette; Ezzedine Aouani
Source:: Biochimica et biophysica acta 2017 v.1865 no.5 pp. 578-588
ISSN:: 1570-9639
Subject:: animal models; brain; calcium signaling; cytoskeleton; glycolysis; grape seeds; grapes; high fat diet; lipotoxicity; mass spectrometry; obesity; oxidative stress; protective effect; proteins; proteomics; rats
Abstract:: ... In recent years, the obesity epidemic has developed into a major health crisis worldwide. With current treatments limited to expensive, high-risk surgery and minimally efficacious pharmacotherapy, new therapeutic options are urgently needed to fight against this alarming trend. Though brain dysfunction has been studied linked to high fat diet (HFD) and grape seed and skin extract (GSSE) correction ...
DOI:: 10.1016/j.bbapap.2017.03.001
: https://doi.org/10.1016/j.bbapap.2017.03.001

32. Buyang Huanwu decoction facilitates neurorehabilitation through an improvement of synaptic plasticity in cerebral ischemic rats

Author:: Ruihuan Pan; Jun Cai; Lechang Zhan; Youhua Guo; Run-Yue Huang; Xiong Li; Mingchao Zhou; Dandan Xu; Jie Zhan; Hongxia Chen
Source:: BMC complementary and alternative medicine 2017 v.17 no.1 pp. 173
ISSN:: 1472-6882
Subject:: Oriental traditional medicine; Western blotting; alternative medicine; brain; cytoskeleton; exercise; immunohistochemistry; infarction; ischemia; neurons; neuroplasticity; proteins; rats; stroke; transmission electron microscopy; ultrastructure
Abstract:: ... BACKGROUND: Loss of neural function is a critical but unsolved issue after cerebral ischemia insult. Neuronal plasticity and remodeling are crucial for recovery of neural functions after brain injury. Buyang Huanwu decoction, which is a classic formula in traditional Chinese medicine, can positively alter synaptic plasticity. This study assessed the effects of Buyang Huanwu decoction in combinatio ...
DOI:: 10.1186/s12906-017-1680-9
PubMed:: 28351388
PubMed Central:: PMC5371213
: https://doi.org/10.1186/s12906-017-1680-9

33. Calpain inhibition prevents flotillin re-ordering and Src family activation during capacitation

Author:: Deneb Maldonado-García; Monica L. Salgado-Lucio; Ana L. Roa-Espitia; Tania Reyes-Miguel; Enrique O. Hernández-González
Source:: Cell and tissue research 2017 v.369 no.2 pp. 395-412
ISSN:: 0302-766X
Subject:: acrosome reaction; calpain; cytoskeleton; guinea pigs; phosphotransferases (kinases); protein phosphorylation; spectrin; spermatozoa
Abstract:: ... Prior to fertilization, mammalian sperm undergo several molecular, biochemical and physiological changes in a process termed capacitation. However, the mechanisms explaining the involvement of cytoskeletal remodeling and membrane re-ordering in each process prior to fertilization remain poorly understood. We found that the migration of both flotillin microdomains and Src family kinases towards the ...
DOI:: 10.1007/s00441-017-2591-2
: https://doi.org/10.1007/s00441-017-2591-2

34. Candida albicans Modifies the Protein Composition and Size Distribution of THP-1 Macrophage-Derived Extracellular Vesicles

Author:: Reales-Calderón Jose Antonio; Vaz Catarina; Monteoliva Lucía; Molero Gloria; Gil Concha
Source:: Journal of Proteome Research 2017 v.16 no.1 pp. 87-105
ISSN:: 1535-3907
Subject:: Candida albicans; candidiasis; cytoskeleton; immune response; lipids; macrophages; messenger RNA; mitogen-activated protein kinase; monocytes; non-coding RNA; pathogens; protein composition; protein secretion; proteins; proteome; proteomics; tumor necrosis factor-alpha
Abstract:: ... The effectiveness of macrophages in the response to systemic candidiasis is crucial to an effective clearance of the pathogen. The secretion of proteins, mRNAs, noncoding RNAs and lipids through extracellular vesicles (EVs) is one of the mechanisms of communication between immune cells. EVs change their cargo to mediate different responses, and may play a role in the response against infections. T ...
DOI:: 10.1021/acs.jproteome.6b00605
: https://doi.org/10.1021/acs.jproteome.6b00605

35. Canonical and cellular pathways timing gamete release in Acropora digitifera, Okinawa, Japan

Author:: Y. Rosenberg; T. Doniger; S. Harii; F. Sinniger; O. Levy
Source:: Molecular ecology 2017 v.26 no.10 pp. 2698-2710
ISSN:: 0962-1083
Subject:: Acropora; brooders; calcium signaling; cell movement; corals; cytoskeleton; focal adhesions; gene expression regulation; plants (botany); receptors; reproduction; reproductive behavior; solar radiation; spawning; transcriptome; Ryukyu Archipelago
Abstract:: ... Natural light cycles are important for synchronizing behavioural and physiological rhythms over varying time periods in both plants and animals. An endogenous clock, regulated by positive and negative elements, interacting in feedback loops controls these rhythms. Many corals exhibit diel cycles of polyp expansion and contraction entrained by solar light patterns and monthly cycles of spawning or ...
DOI:: 10.1111/mec.14062
: https://doi.org/10.1111/mec.14062

36. Carbon black aggregates cause endothelial dysfunction by activating ROCK

Author:: Junyan Yan; Chia-Hsiang Lai; Shih-Chun Candice Lung; Wen-Cheng Wang; Chih-Ching Huang; Guan-Wen Chen; Guangli Suo; Cheng-Tai Choug; Chia-Hua Lin
Source:: Journal of hazardous materials 2017 v.338 pp. 66-75
ISSN:: 0304-3894
Subject:: adverse effects; atherosclerosis; blood flow; cell viability; cytoskeleton; human cell lines; lactate dehydrogenase; nanoparticles; oxidative stress; soot; toxicity; transmission electron microscopy
Abstract:: ... Carbon black nanoparticles (CBNs) have been associated with the progression of atherosclerosis. CBNs normally enter the bloodstream and crosslink together to form agglomerates. However, most studies have used nano-sized CB particles to clarify the involvement of CBN exposure in CBN-induced endothelial dysfunction. Herein, we studied endothelial toxicity of CBN aggregates (CBA) to human EA.hy926 va ...
DOI:: 10.1016/j.jhazmat.2017.05.025
: https://doi.org/10.1016/j.jhazmat.2017.05.025

37. Cell polarity in plants: the Yin and Yang of cellular functions

Author:: Jiyan Qi; Thomas Greb
Source:: Current opinion in plant biology 2017 v.35 pp. 105-110
ISSN:: 1369-5266
Subject:: cell polarity; chemical elements; cytoskeleton; eukaryotic cells; extracellular matrix; organelles
Abstract:: ... Spatial organization is fundamental for the performance of living organisms and is reflected in a distinct distribution of structures and molecules down to the subcellular level. In particular, eukaryotic cells harbor a vast range of possibilities for distributing organelles, the cytoskeleton or the extracellular matrix in an active and highly regulated manner. An asymmetric or polar distribution ...
DOI:: 10.1016/j.pbi.2016.11.015
PubMed:: 27918938
PubMed Central:: PMC7212042
: https://doi.org/10.1016/j.pbi.2016.11.015

38. Cell wall accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated Arabidopsis leaf epidermal cells

Author:: Kae Akita; Megumi Kobayashi; Mayuko Sato; Natsumaro Kutsuna; Takashi Ueda; Kiminori Toyooka; Noriko Nagata; Seiichiro Hasezawa; Takumi Higaki
Source:: Protoplasma 2017 v.254 no.1 pp. 367-377
ISSN:: 0033-183X
Subject:: Arabidopsis thaliana; cell walls; cytoskeleton; exocytosis; fluorescence; fluorescent proteins; guard cells; image analysis; leaf development; leaves; morphogenesis; plasma membrane; rats; transferases; transmission electron microscopy
Abstract:: ... In most dicotyledonous plants, leaf epidermal pavement cells develop jigsaw puzzle-like shapes during cell expansion. The rapid growth and complicated cell shape of pavement cells is suggested to be achieved by targeted exocytosis that is coordinated with cytoskeletal rearrangement to provide plasma membrane and/or cell wall materials for lobe development during their morphogenesis. Therefore, vis ...
DOI:: 10.1007/s00709-016-0955-1
: https://doi.org/10.1007/s00709-016-0955-1

39. Cellular and molecular characterizations of a slow-growth variant provide insights into the fast growth of bamboo

Author:: Qiang Wei; Chen Jiao; Yulong Ding; Shan Gao; Lin Guo; Ming Chen; Pei Hu; Sujuan Xia; Guodong Ren; Zhangjun Fei
Source:: Tree physiology 2017 v.38 no.4 pp. 641-654
ISSN:: 1758-4469
Subject:: Pseudosasa; auxins; bamboos; cell division; cell growth; cell walls; cellulose; cytoskeleton; developmental stages; extrusion; gene expression regulation; genes; genomics; internodes; mathematics; mutants; physiological transport; transcriptomics; tree physiology
Abstract:: ... Few studies about bamboo naturally occurring mutants have been reported so far. Using an integrated anatomy, mathematics and genomics approach, we systematically characterized the cellular and molecular basis underlying the abnormal internode development of Pseudosasa japonica var. tsutsumiana, a stable variant with dwarf and swollen internodes that are caused by the compressed spiral growth and t ...
DOI:: 10.1093/treephys/tpx129
: https://doi.org/10.1093/treephys/tpx129

40. Cerium oxide nanoparticles (CeO2 NPs) improve the developmental competence of in vitro-matured prepubertal ovine oocytes

Author:: F. Ariu; L. Bogliolo; A. Pinna; L. Malfatti; P. Innocenzi; L. Falchi; D. Bebbere; S. Ledda
Source:: Reproduction, fertility, and development 2017 v.29 no.5 pp. 1046-1056
ISSN:: 1031-3613
Subject:: B-lymphocytes; blastocyst; ceric oxide; chromatin; cytoskeleton; embryogenesis; lymphoma; messenger RNA; nanoparticles; oocytes; puberty; sheep; superoxide dismutase; zygote
Abstract:: ... The present study investigated whether supplementation with different doses of cerium dioxide nanoparticles (CeO2 NPs) during in vitro maturation (IVM) of prepubertal ovine oocytes influenced their embryonic development in vitro. Cumulus–oocyte complexes derived from the ovaries of slaughtered prepubertal sheep underwent IVM with CeO2NPs (0, 44, 88 or 220µg mL-1). Matured oocytes were fertilised i ...
DOI:: 10.1071/RD15521
: https://doi.org/10.1071/RD15521

41. Changes in the cerebellar cytoarchitecture of hibernating hedgehog Erinaceus europaeus L. (Mammalia): an immunocytochemical approach

Author:: E. Roda; M. G. Bottone; V. Insolia; S. Barni; G. Bernocchi
Source:: The European zoological journal 2017 v.84 no.1 pp. 496-511
ISSN:: 2475-0263
Subject:: Erinaceus europaeus; ambient temperature; axons; calbindin; calcium; cerebellum; cerebral cortex; cytoskeleton; death; glutamate decarboxylase; glutamic acid; hibernation; homeostasis; immunohistochemistry; mammals; models; neurotransmitters; synaptic transmission
Abstract:: ... Hibernation is an amazing animal strategy to survive when the environmental temperature is very low and food resources are scarce. Successful hibernation requires a variety of complex biological adaptations, in which the brain plays a central regulatory role. Currently, little information is available regarding the morphology and functional activity of specific neurons within the cerebellar cytoar ...
DOI:: 10.1080/24750263.2017.1380722
: https://doi.org/10.1080/24750263.2017.1380722

42. Characterization of the Molecular Mechanisms Underlying the Chronic Phase of Stroke in a Cynomolgus Monkey Model of Induced Cerebral Ischemia

Author:: Law Henry C. H.; Szeto Samuel S. W.; Quan Quan; Zhao Yun; Zhang Zaijun; Krakovska Olga; Lui Leong Ting; Zheng Chengyou; Lee Simon M.-Y.; Siu K. W. Michael; Wang Yuqiang; Chu Ivan K.
Source:: Journal of Proteome Research 2017 v.16 no.3 pp. 1150-1166
ISSN:: 1535-3907
Subject:: Macaca fascicularis; cerebral cortex; cytoskeleton; infarction; inflammation; ischemia; liquid chromatography; models; mortality; neurogenesis; proteins; proteome; proteomics; stroke; synaptogenesis
Abstract:: ... Stroke is one of the main causes of mortality and long-term disability worldwide. The pathophysiological mechanisms underlying this disease are not well understood, particularly in the chronic phase after the initial ischemic episode. In this study, a Macaca fascicularis stroke model consisting of two sample groups, as determined by MRI-quantified infarct volumes as a measure of the stroke severit ...
DOI:: 10.1021/acs.jproteome.6b00651
: https://doi.org/10.1021/acs.jproteome.6b00651

43. Cleavage of β-dystroglycan occurs in sarcoglycan-deficient skeletal muscle without MMP-2 and MMP-9

Author:: Yuta Fukai; Yutaka Ohsawa; Hideaki Ohtsubo; Shin-ichiro Nishimatsu; Hiroki Hagiwara; Makoto Noda; Toshikuni Sasaoka; Tatsufumi Murakami; Yoshihide Sunada
Source:: Biochemical and biophysical research communications 2017 pp. -
ISSN:: 0006-291X
Subject:: cytoskeleton; extracellular matrix; gene expression regulation; in vitro studies; knockout mutants; metalloproteinases; mice; muscles; muscular dystrophy; pathogenesis; patients; proteinase inhibitors; skeletal muscle
Abstract:: ... The dystroglycan complex consists of two subunits: extracellular α-dystroglycan and membrane-spanning β-dystroglycan, which provide a tight link between the extracellular matrix and the intracellular cytoskeleton. Previous studies showed that 43 kDa β-dystroglycan is proteolytically cleaved into the 30 kDa fragment by matrix metalloproteinases (MMPs) in various non-muscle tissues, whereas it is pr ...
DOI:: 10.1016/j.bbrc.2017.08.048
: https://doi.org/10.1016/j.bbrc.2017.08.048

44. Clinical and histopathological features of myofibrillar myopathy in Warmblood horses

Author:: S. J. Valberg; A. M. Nicholson; S. S. Lewis; R. A. Reardon; C. J. Finno
Source:: Equine veterinary journal 2017 v.49 no.6 pp. 739-745
ISSN:: 0425-1644
Subject:: atrophy; biopsy; cytoskeleton; desmin; dystrophin; electron microscopy; exercise; gait; glycogen; histopathology; horses; lameness; muscle tissues; muscles; muscular diseases; myosin; protein aggregates
Abstract:: ... BACKGROUND: To report a novel exertional myopathy, myofibrillar myopathy (MFM) in Warmblood (WB) horses. OBJECTIVES: To 1) describe the distinctive clinical and myopathic features of MFM in Warmblood horses and 2) investigate the potential inheritance of MFM in a Warmblood family. STUDY DESIGN: Retrospective selection of MFM cases and prospective evaluation of a Warmblood family. METHODS: Retrospe ...
DOI:: 10.1111/evj.12702
PubMed:: 28543538
PubMed Central:: PMC5640499
: https://doi.org/10.1111/evj.12702

45. Cloning, characterization and subcellular localization of Nuclear LIM interactor interacting factor gene from Leishmania donovani

Author:: R Ravinder; N Goyal
Source:: Gene 2017 v.611 pp. 1-8
ISSN:: 0378-1119
Subject:: Leishmania donovani; cytoskeleton; gene expression; genes; neoplasms; nuclear proteins; parasites; phylogeny; protein-protein interactions; sequence analysis; transcription factors; visceral leishmaniasis
Abstract:: ... LIM domains are zinc-binding motifs that mediate protein–protein interactions and are found in a wide variety of cytoplasmic and nuclear proteins. The nuclear LIM domain family members have a number of different functions including transcription factors, gene regulation, cell fate determination, organization of the cytoskeleton and tumour formation exerting their function through various LIM domai ...
DOI:: 10.1016/j.gene.2017.02.007
: https://doi.org/10.1016/j.gene.2017.02.007

46. Co-Delivery of Trichosanthin and Albendazole by Nano-Self-Assembly for Overcoming Tumor Multidrug-Resistance and Metastasis

Author:: Tang Yisi; Liang Jianming; Wu Aihua; Chen Yingzhi; Zhao Pengfei; Lin Tingting; Zhang Meng; Xu Qin; Wang Jianxin; Huang Yongzhuo
Source:: ACS Applied Materials & Interfaces 2017 v.9 no.32 pp. 26648-26664
ISSN:: 1944-8252
Subject:: albendazole; animal models; antineoplastic activity; antineoplastic agents; apoptosis; cell cycle; combination drug therapy; cytoskeleton; half life; in vivo studies; metastasis; multiple drug resistance; nanosilver; neoplasm cells; neoplasms; pharmacokinetics; polymerization; tubulin
Abstract:: ... Multidrug resistance (MDR) and metastasis are the major obstacles in cancer chemotherapy. Nanotechnology-based combination therapy is a useful strategy. Recently, the combination of biologics and small drugs has attracted much attention in cancer therapy. Yet, the treatment outcomes are often compromised by the different pharmacokinetic profiles of the co-administered drugs thus leading to inconsi ...
DOI:: 10.1021/acsami.7b05292
: https://doi.org/10.1021/acsami.7b05292

47. Coenzyme Q10 supplementation during in vitro maturation of bovine oocytes (Bos taurus) helps to preserve oocyte integrity after vitrification

Author:: M Ruiz‐Conca; M Vendrell; M Sabés‐Alsina; T Mogas; M Lopez‐Bejar
Source:: Reproduction in domestic animals 2017 v.52 Suppl S4 no. pp. 52-54
ISSN:: 0936-6768
Subject:: antioxidants; cattle; coenzyme Q10; cooling; cortical granules; cytoskeleton; embryogenesis; exocytosis; mice; oocytes; plasma membrane; vitrification
Abstract:: ... Oocyte vitrification causes less cell stress than slow cooling, but cytoskeletal and spindle alterations may occur affecting the oocyte competence. In vitro maturation (IVM) supplementation with different antioxidant molecules has been performed to attenuate this harmful stress. Coenzyme Q₁₀ (CoQ₁₀) supplementation has previously shown to have positive effects in bovine and mouse in vitro embryo d ...
DOI:: 10.1111/rda.13056
: https://doi.org/10.1111/rda.13056

48. Comparative proteomic analysis provides insight into 10-hydroxy-2-decenoic acid biosynthesis in honey bee workers

Author:: Xiao-Hui Yang; Shi-Fa Yang; Rui-Ming Wang
Source:: Amino acids 2017 v.49 no.7 pp. 1177-1192
ISSN:: 0939-4451
Subject:: RNA interference; beta oxidation; biosynthesis; cytoskeleton; developmental stages; electron transfer; energy metabolism; fatty acid metabolism; fatty-acid synthase; flavoproteins; mandibular glands; protein metabolism; proteomics; secretion; worker honey bees
Abstract:: ... 10-Hydroxy-2-decenoic acid (10-HDA) is the major compound produced from the mandibular glands (MGs) of honey bee workers. However, little information is available on the molecular mechanisms of 10-HDA biosynthesis. In our study, based on investigating the 10-HDA secretion pattern and the morphological characteristics of MGs from honey bee workers of different ages, a comparative proteomic analysis ...
DOI:: 10.1007/s00726-017-2418-1
: https://doi.org/10.1007/s00726-017-2418-1

49. Comparative proteomics reveals the physiological differences between winter tender shoots and spring tender shoots of a novel tea (Camellia sinensis L.) cultivar evergrowing in winter

Author:: Shengjie Liu; Jiadong Gao; Zhongjian Chen; Xiaoyan Qiao; Hualin Huang; Baiyuan Cui; Qingfeng Zhu; Zhangyan Dai; Hualing Wu; Yayan Pan; Chengwei Yang; Jun Liu
Source:: BMC plant biology 2017 v.17 no.1 pp. 206
ISSN:: 1471-2229
Subject:: Camellia sinensis; H+/K+-exchanging ATPase; H-transporting ATP synthase; amino acids; cultivars; cytoskeleton; desorption; flavonoids; histones; leaves; matrix-assisted laser desorption-ionization mass spectrometry; photosynthesis; plastocyanin; polyphenols; polysaccharides; profilins; protein synthesis; proteinases; proteomics; ribulose-bisphosphate carboxylase; shoots; spring; sugars; tea; temperature; transporters; winter
Abstract:: ... BACKGROUND: A recently discovered tea [Camellia sinensis (L.) O. Kuntze] cultivar can generate tender shoots in winter. We performed comparative proteomics to analyze the differentially accumulated proteins between winter and spring tender shoots of this clonal cultivar to reveal the physiological basis of its evergrowing character during winter. RESULTS: We extracted proteins from the winter and ...
DOI:: 10.1186/s12870-017-1144-x
PubMed:: 29157222
PubMed Central:: PMC5697017
: https://doi.org/10.1186/s12870-017-1144-x

50. Comprehensive evaluation of gene expression signatures in response to electroacupuncture stimulation at Zusanli (ST36) acupoint by transcriptomic analysis

Author:: Jing-Shan Wu; Hsin-Yi Lo; Chia-Cheng Li; Feng-Yuan Chen; Chien-Yun Hsiang; Tin-Yun Ho
Source:: BMC complementary and alternative medicine 2017 v.17 no.1 pp. 406
ISSN:: 1472-6882
Subject:: acupuncture; brain; cell adhesion; cytoskeleton; gene expression; inflammation; mice; microarray technology; neurodegenerative diseases; prediction; respiratory tract diseases; tissues; transcriptomics
Abstract:: ... BACKGROUND: Electroacupuncture (EA) has been applied to treat and prevent diseases for years. However, molecular events happened in both the acupunctured site and the internal organs after EA stimulation have not been clarified. METHODS: Here we applied transcriptomic analysis to explore the gene expression signatures after EA stimulation. Mice were applied EA stimulation at ST36 for 15 min and ni ...
DOI:: 10.1186/s12906-017-1911-0
PubMed:: 28810910
PubMed Central:: PMC5556347
: https://doi.org/10.1186/s12906-017-1911-0

51. Contributions of polyunsaturated fatty acids (PUFA) on cerebral neurobiology: an integrated omics approach with epigenomic focus

Author:: Nabarun Chakraborty; Seid Muhie; Raina Kumar; Aarti Gautam; Seshamalini Srinivasan; Bintu Sowe; George Dimitrov; Stacy-Ann Miller; Marti Jett; Rasha Hammamieh
Source:: Journal of nutritional biochemistry 2017 v.42 pp. 84-94
ISSN:: 0955-2863
Subject:: Alzheimer disease; DNA methylation; cognition; cytoskeleton; dietary supplements; epigenetics; genes; genomic islands; memory; mice; microRNA; neurodegenerative diseases; neurodevelopment; neurons; neurotransmitters; nutrigenomics; omega-3 fatty acids; omega-6 fatty acids; polyunsaturated fatty acids; schizophrenia; synaptogenesis; transcription (genetics); transcriptomics
Abstract:: ... The epigenetic landscape is vulnerable to diets. Here, we investigated the influence of different polyunsaturated fatty acids (PUFA) dietary supplements on rodents' nervous system development and functions and potential consequences to neurodegenerative disorders. Our previous nutrigenomics study showed significant impact of high n-3 PUFA-enriched diet (ERD) on synaptogenesis and various neuromodu ...
DOI:: 10.1016/j.jnutbio.2016.12.006
: https://doi.org/10.1016/j.jnutbio.2016.12.006

52. Control of nuclear organization by F-actin binding proteins

Author:: Karin Pfisterer; Asier Jayo; Maddy Parsons
Source:: Nucleus 2017 v.8 no.2 pp. 126-133
ISSN:: 1949-1042
Subject:: actin; cytoskeleton; embryogenesis; metastasis; neoplasm cells; nuclear membrane; plasticity; pseudopodia
Abstract:: ... The regulation of nuclear shape and deformability is a key factor in controlling diverse events from embryonic development to cancer cell metastasis, but the mechanisms governing this process are still unclear. Our recent study demonstrated an unexpected role for the F-actin bundling protein fascin in controlling nuclear plasticity through a direct interaction with Nesprin-2. Nesprin-2 is a compon ...
DOI:: 10.1080/19491034.2016.1267093
PubMed:: 28060557
PubMed Central:: PMC5403142
: https://doi.org/10.1080/19491034.2016.1267093

53. Controlling cell volume for efficient PHB production by Halomonas

Author:: Xiao-Ran Jiang; Zhi-Hao Yao; Guo-Qiang Chen
Source:: Metabolic engineering 2017 v.44 pp. 30-37
ISSN:: 1096-7176
Subject:: Halomonas; bioreactors; cell division; cell growth; cytoskeleton; essential genes; gravity; plasmids; polyhydroxybutyrate
Abstract:: ... Bacterial morphology is decided by cytoskeleton protein MreB and cell division protein FtsZ encoded by essential genes mreB and ftsZ, respectively. Inactivating mreB and ftsZ lead to increasing cell sizes and cell lengths, respectively, yet seriously reduce cell growth ability. Here we develop a temperature-responsible plasmid expression system for compensated expression of relevant gene(s) in mre ...
DOI:: 10.1016/j.ymben.2017.09.004
: https://doi.org/10.1016/j.ymben.2017.09.004

54. Coordinate expression of pan-neuronal and functional signature genes in sympathetic neurons

Author:: U. Ernsberger; M. Kramer; K. Tsarovina; T. Deller; H. Rohrer
Source:: Cell and tissue research 2017 v.370 no.2 pp. 227-241
ISSN:: 0302-766X
Subject:: cytoskeleton; enzymes; ganglia; gene expression; genes; genetic markers; ion channels; mice; neurons; neurotransmitters; sequence analysis; transporters; tubulin
Abstract:: ... Neuron subtypes of the mature nervous system differ in the expression of characteristic marker genes while they share the expression of generic neuronal genes. The regulatory logic that maintains subtype-specific and pan-neuronal genes is not well understood. To begin to address this issue, we analyze RNA sequencing results from whole sympathetic ganglia and single sympathetic neurons in the mouse ...
DOI:: 10.1007/s00441-017-2688-7
: https://doi.org/10.1007/s00441-017-2688-7

55. Critical role of Keratin 1 in maintaining epithelial barrier and correlation of its down-regulation with the progression of inflammatory bowel disease

Author:: Xiangqian Dong; Zichao Liu; Danfeng Lan; Junkun Niu; Jiarong Miao; Gang Yang; Fengrui Zhang; Yang Sun; Kunhua Wang; Yinglei Miao
Source:: Gene 2017 v.608 pp. 13-19
ISSN:: 0378-1119
Subject:: Crohn disease; Western blotting; blood serum; colitis; colon; cytoskeleton; epithelium; human cell lines; immunohistochemistry; inflammation; interleukin-1beta; keratin; patients; reverse transcriptase polymerase chain reaction; small intestine; tight junctions
Abstract:: ... Inflammatory bowel disease (IBD) is the result of a chronic intestinal inflammatory response which usually occurred in colon and small intestine. Keratins constitute the intermediate filament cytoskeleton in all epithelia. The present study was intended to explore the role of Keratin 1 (KRT1) in the progress of IBD. In normal intestinal tissue, the expression of KRT1 was detected by RT-PCR and Wes ...
DOI:: 10.1016/j.gene.2017.01.015
: https://doi.org/10.1016/j.gene.2017.01.015

56. Crosstalk of cell polarity signaling pathways

Author:: Tomáš Mazel
Source:: Protoplasma 2017 v.254 no.3 pp. 1241-1258
ISSN:: 0033-183X
Subject:: cell movement; cell polarity; chemical elements; cytoskeleton; epithelium; guanosinetriphosphatase; lipid composition; lipids; messenger RNA; neurons; oocytes; organelles; pheromones; physiological transport; plasma membrane; receptors; signal transduction; yeasts
Abstract:: ... Cell polarity, the asymmetric organization of cellular components along one or multiple axes, is present in most cells. From budding yeast cell polarization induced by pheromone signaling, oocyte polarization at fertilization to polarized epithelia and neuronal cells in multicellular organisms, similar mechanisms are used to determine cell polarity. Crucial role in this process is played by signal ...
DOI:: 10.1007/s00709-017-1075-2
: https://doi.org/10.1007/s00709-017-1075-2

57. Cryopreservation of bull semen is associated with carbonylation of sperm proteins

Author:: Agnieszka Mostek; Mariola Aleksandra Dietrich; Mariola Słowińska; Andrzej Ciereszko
Source:: Theriogenology 2017 v.92 pp. 95-102
ISSN:: 0093-691X
Subject:: NADH dehydrogenase; Western blotting; artificial insemination; bulls; cryopreservation; cytoskeleton; desorption; energy metabolism; flagellum; flow cytometry; glutathione transferase; isocitrate dehydrogenase; oxidation; oxidative stress; proteins; reactive oxygen species; semen; spectroscopy; sperm motility; spermatozoa; triose-phosphate isomerase; two-dimensional gel electrophoresis; viability
Abstract:: ... Artificial insemination with cryopreserved semen enables affordable, large-scale dissemination of gametes with superior genetics. However, cryopreservation can cause functional and structural damage to spermatozoa that is associated with reactive oxygen species (ROS) production, impairment of sperm motility and decreased fertilizing potential, but little attention has been paid to protein changes. ...
DOI:: 10.1016/j.theriogenology.2017.01.011
: https://doi.org/10.1016/j.theriogenology.2017.01.011

58. Curcumin alters the cytoskeleton and microtubule organization on trophozoites of Giardia lamblia

Author:: Filiberto Gutiérrez-Gutiérrez; Lissethe Palomo-Ligas; José Manuel Hernández-Hernández; Armando Pérez-Rangel; Rodrigo Aguayo-Ortiz; Alicia Hernández-Campos; Rafael Castillo; Sirenia González-Pozos; Rafael Cortés-Zárate; Mario Alberto Ramírez-Herrera; María Luisa Mendoza-Magaña; Araceli Castillo-Romero
Source:: Acta tropica 2017 pp. -
ISSN:: 0001-706X
Subject:: Giardia lamblia; adhesion; adverse effects; binding sites; confocal microscopy; curcumin; flagellum; fluorescent antibody technique; giardiasis; microtubules; molecular dynamics; parasites; probability; scanning electron microscopy; staining; trophozoites; tubulin; turmeric; vinblastine
Abstract:: ... Giardia lamblia is a worldwide protozoan responsible for a significant number of intestinal infections. There are several drugs for the treatment of giardiasis, but they often cause side effects. Curcumin, a component of turmeric, has antigiardial activity; however, the molecular target and mechanism of antiproliferative activity are not clear. The effects of curcumin on cellular microtubules have ...
DOI:: 10.1016/j.actatropica.2017.04.027
: https://doi.org/10.1016/j.actatropica.2017.04.027

59. Curdione attenuates thrombin-induced human platelet activation: β1-tubulin as a potential therapeutic target

Author:: Dongling Zhang; Wenhao Qiao; Yingli Zhao; Hui Fang; Dujuan Xu; Quan Xia
Source:: Fitoterapia 2017 v.116 pp. 106-115
ISSN:: 0367-326X
Subject:: Curcuma aromatica; Western blotting; anticoagulants; bioinformatics; blood stasis; computer software; cytoskeleton; databases; essential oils; humans; integrins; medicinal plants; pain; platelet activation; protein synthesis; proteomics; rhizomes; sesquiterpenoids; signal transduction; thrombin
Abstract:: ... Rhizoma Curcumae, the dry rhizomes derived from Curcuma aromatica Salisb., are a classical Chinese medicinal herb used to activate blood circulation, remove blood stasis and alleviate pain. Our previous study proved that curdione, a sesquiterpene compound isolated from the essential oil of Curcuma aromatica Salisb. can inhibit platelet activation suggesting its significant anticoagulant and antith ...
DOI:: 10.1016/j.fitote.2016.11.016
: https://doi.org/10.1016/j.fitote.2016.11.016

60. Cyclin-dependent kinase 5 controls vasculogenic mimicry formation in non-small cell lung cancer via the FAK-AKT signaling pathway

Author:: Xiaoshu Zhou; Runxia Gu; Xiaoming Han; Gang Wu; Junli Liu
Source:: Biochemical and biophysical research communications 2017 v.492 no.3 pp. 447-452
ISSN:: 0006-291X
Subject:: Western blotting; cell lines; cyclin-dependent kinase; cytoskeleton; immunohistochemistry; lung neoplasms; neoplasm cells; neuroplasticity; phosphorylation; prognosis; recombinant proteins; signal transduction; small interfering RNA
Abstract:: ... Vasculogenic mimicry (VM), an endothelial-independent tumor vascularization phenomenon representing functional tumor plasticity, might be the culprit behind the poor clinical outcome in classic antiangiogenesis treatment. However, the mechanism underlying VM needs to be elucidated. Cyclin-dependent kinase 5 (CDK5) has been recognized as a key factor in regulating migration and neuronal plasticity. ...
DOI:: 10.1016/j.bbrc.2017.08.076
: https://doi.org/10.1016/j.bbrc.2017.08.076

61. Cytoskeleton and nuclear lamina affection in recessive osteogenesis imperfecta: A functional proteomics perspective

Author:: Assunta Gagliardi; Roberta Besio; Chiara Carnemolla; Claudia Landi; Alessandro Armini; Mona Aglan; Ghada Otaify; Samia A. Temtamy; Antonella Forlino; Luca Bini; Laura Bianchi
Source:: Journal of proteomics 2017 v.167 pp. 46-59
ISSN:: 1874-3919
Subject:: Western blotting; antibodies; assets; bone formation; collagen; cytoskeleton; drugs; endoplasmic reticulum; fibroblasts; fluorescent antibody technique; genes; metabolism; mutation; pathophysiology; patients; phenotype; proteomics; prototypes; structural proteins
Abstract:: ... Osteogenesis imperfecta (OI) is a collagen-related disorder associated to dominant, recessive or X-linked transmission, mainly caused by mutations in type I collagen genes or in genes involved in type I collagen metabolism.Among the recessive forms, OI types VII, VIII, and IX are due to mutations in CRTAP, P3H1, and PPIB genes, respectively. They code for the three components of the endoplasmic re ...
DOI:: 10.1016/j.jprot.2017.08.007
PubMed:: 28802583
PubMed Central:: PMC5584732
: https://doi.org/10.1016/j.jprot.2017.08.007

62. Cytoskeleton reorganization/disorganization is a key feature of induced inaccessibility for defence to successive pathogen attacks

Author:: Juan Moral; Gracia Montilla‐Bascón; Francisco J. Canales; Diego Rubiales; Elena Prats
Source:: Molecular plant pathology 2017 v.18 no.5 pp. 662-671
ISSN:: 1464-6722
Subject:: Erysiphe pisi; actin; cycloheximide; cytochalasin D; cytoskeleton; fungi; induced resistance; leaves; mixed infection; pathogens; peas; powdery mildew; resistance to penetration
Abstract:: ... In this work, we investigated the involvement of the long‐term dynamics of cytoskeletal reorganization on the induced inaccessibility phenomenon by which cells that successfully defend against a previous fungal attack become highly resistant to subsequent attacks. This was performed on pea through double inoculation experiments using inappropriate (Blumeria graminis f. sp. avenae, Bga) and appropr ...
DOI:: 10.1111/mpp.12424
PubMed:: 27147535
PubMed Central:: PMC6638220
: https://doi.org/10.1111/mpp.12424

63. Cytotoxicity of thirteen South African macrofungal species against five cancer cell lines

Author:: G.J. Boukes; T.C. Koekemoer; M. van de Venter; S. Govender
Source:: South African journal of botany 2017 v.113 pp. 62-67
ISSN:: 0254-6299
Subject:: Fomitopsis; Gymnopilus; Pycnoporus sanguineus; apoptosis; chromatin; cytoskeleton; cytotoxicity; fungi; humans; inhibitory concentration 50; neoplasm cells; neoplasms; staining; South Africa
Abstract:: ... Fungi are an important source of traditional medicine in Asian and Western countries. Globally, cancer is one of the leading causes of death with 22 million cases predicted by 2030. The cytotoxicity of 13 South African macrofungal species against HeLa, HT-29, MCF-7, MIA PaCa-2 and PC-3 cancer cell lines was measured using the 3-(4,5-dimethyl-2-thiazolul)-2,5-diphenyl-2H-tetrazolium bromide (MTT) a ...
DOI:: 10.1016/j.sajb.2017.07.010
: https://doi.org/10.1016/j.sajb.2017.07.010

64. Daboialectin, a C-type lectin from Russell's viper venom induces cytoskeletal damage and apoptosis in human lung cancer cells in vitro

Author:: Jigni Pathan; Sukanta Mondal; Angshuman Sarkar; Dibakar Chakrabarty
Source:: Toxicon 2017 v.127 pp. 11-21
ISSN:: 0041-0101
Subject:: DNA damage; Daboia russelii; apoptosis; carcinoma; caspases; cell growth; cytoskeleton; cytotoxicity; guanosinetriphosphatase; humans; lectins; lung neoplasms; molecular weight; neoplasm cells; pro-apoptotic proteins; snakes; venoms
Abstract:: ... ‘Daboialectin’, a low molecular weight C-type lectin (18.5 kDa) isolated from Russell's viper venom showed cytotoxic effects on human broncho-alveolar carcinoma derived (A549) cell lines. Daboialectin induced inhibition of A549 cell growth was time and concentration dependent with severe morphological changes by altering the functions of small GTPases such as Rac, Rho and cdc42. ROS induced DNA da ...
DOI:: 10.1016/j.toxicon.2016.12.013
: https://doi.org/10.1016/j.toxicon.2016.12.013

65. Data on evolution of intrinsically disordered regions of the human kinome and contribution of FAK1 IDRs to cytoskeletal remodeling

Author:: Jaymin J. Kathiriya; Ravi Ramesh Pathak; Alexandr Bezginov; Bin Xue; Vladimir N. Uversky; Elisabeth R.M. Tillier; Vrushank Davé
Source:: Data in Brief 2017 v.10 pp. 315-324
ISSN:: 2352-3409
Subject:: cytoskeleton; evolution; humans; hybrids; phosphotransferases (kinases); protein-protein interactions
Abstract:: ... We present data on the evolution of intrinsically disordered regions (IDRs) taking into account the entire human protein kinome. The evolutionary data of the IDRs with respect to the kinase domains (KDs) and kinases as a whole protein (WP) are reported. Further, we have reported its post translational modifications of FAK1 IDRs and their contribution to the cytoskeletal remodeling. We also report ...
DOI:: 10.1016/j.dib.2016.11.099
: https://doi.org/10.1016/j.dib.2016.11.099

66. Deciphering the BAR code of membrane modulators

Author:: Ulrich Salzer; Julius Kostan; Kristina Djinović-Carugo
Source:: Cellular and molecular life sciences 2017 v.74 no.13 pp. 2413-2438
ISSN:: 1420-682X
Subject:: cytoskeleton; lipids; post-translational modification; proteins
Abstract:: ... The BAR domain is the eponymous domain of the “BAR-domain protein superfamily”, a large and diverse set of mostly multi-domain proteins that play eminent roles at the membrane cytoskeleton interface. BAR domain homodimers are the functional units that peripherally associate with lipid membranes and are involved in membrane sculpting activities. Differences in their intrinsic curvatures and lipid-b ...
DOI:: 10.1007/s00018-017-2478-0
PubMed:: 28243699
PubMed Central:: PMC5487894
: https://doi.org/10.1007/s00018-017-2478-0

67. Deciphering the protein–protein interaction network regulating hepatocellular carcinoma metastasis

Author:: Guoxuan Qin; Mengjiao Dang; Huajun Gao; Hao Wang; Fengting Luo; Ruibing Chen
Source:: Biochimica et biophysica acta 2017 v.1865 no.9 pp. 1114-1122
ISSN:: 1570-9639
Subject:: Western blotting; bioinformatics; cytoskeleton; hepatoma; metabolism; metastasis; protein-protein interactions; proteins; proteome; proteomics; proto-oncogenes; pyruvate kinase; survival rate
Abstract:: ... Hepatocellular carcinoma (HCC) is one of the leading causes of mortality related to cancer all over the world. To better understand the molecular mechanisms of HCC metastasis, we analyzed the proteome of three HCC cell lines with different metastasis potentials by quantitative proteomics and bioinformatics analysis. As a result, we identified 378 cellular proteins potentially associated to HCC met ...
DOI:: 10.1016/j.bbapap.2017.06.005
: https://doi.org/10.1016/j.bbapap.2017.06.005

68. Deep transcriptome profiling sheds light on key players in nucleus implantation induced immune response in the pearl oyster Pinctada martensii

Author:: Wei Wang; Yuyuan Wu; Qiannan Lei; Haiying Liang; Yuewen Deng
Source:: Fish & shellfish immunology 2017 v.69 pp. 67-77
ISSN:: 1050-4648
Subject:: Pinctada; Toll-like receptors; cell adhesion molecules; cytoskeleton; databases; gene expression; gene expression regulation; immune response; immunosuppression; lectins; metabolism; oyster culture; oysters; pearls; peroxidase; ribosomes; sequence analysis; transcriptomics; unigenes
Abstract:: ... Immunological rejection of the pearl oysters following nucleus implantation is a major issue limiting the successful rate of cultured pearls. To date, the molecular mechanism of immune tolerance during pearl formation in the pearl oysters is still largely unknown. Through the RNA sequencing platform and comparative transcriptomic analysis, we investigated the chronic gene expression changes at sev ...
DOI:: 10.1016/j.fsi.2017.08.011
: https://doi.org/10.1016/j.fsi.2017.08.011

69. Differential Phosphoproteomic Analysis of Recombinant Chinese Hamster Ovary Cells Following Temperature Shift

Author:: Henry Michael; Power Martin; Kaushik Prashant; Coleman Orla; Clynes Martin; Meleady Paula
Source:: Journal of Proteome Research 2017 v.16 no.7 pp. 2339-2358
ISSN:: 1535-3907
Subject:: Chinese hamsters; RNA; Western blotting; apoptosis; bioengineering; biogenesis; biopharmaceutical industry; cell division; cell lines; cell proliferation; cytoskeleton; gene expression regulation; gene ontology; phosphopeptides; phosphoproteins; phosphorylation; post-translational modification; protein-serine-threonine kinases; proteome; proteomics; recombinant proteins; serine; signal transduction; temperature; threonine; titanium dioxide; transcription (genetics); transcription factors
Abstract:: ... Phosphorylation is one of the most important post-translational modifications, playing a crucial role in regulating many cellular processes, including transcription, cytoskeletal rearrangement, cell proliferation, differentiation, apoptosis, and signal transduction. However, to date, little work has been carried out on the phosphoproteome in CHO cells. In this study we have carried out a large sca ...
DOI:: 10.1021/acs.jproteome.6b00868
: https://doi.org/10.1021/acs.jproteome.6b00868

70. Differential gene expression in the endometrium reveals cytoskeletal and immunological genes in lactating dairy cows genetically divergent for fertility traits

Author:: Bruce Moran; Stephen T. Butler; Stephen G. Moore; David E. MacHugh; Christopher J. Creevey
Source:: Reproduction, fertility, and development 2017 v.29 no.2 pp. 274-282
ISSN:: 1031-3613
Subject:: actin; biopsy; breeding; calving interval; cytoskeleton; dairy cows; endometrium; estrous cycle; gene expression; gene expression regulation; genes; genetic merit; immune response; lactating females; lactation; milk production; models; phenotypic variation; reproductive efficiency; reproductive performance; secretion; transcriptome; transportation; transporters; Ireland
Abstract:: ... Profitable milk production in dairy cows requires good reproductive performance. Calving interval is a trait used to measure reproductive efficiency. Herein we used a novel lactating Holstein cow model of fertility that displayed genetic and phenotypic divergence in calving interval, a trait used to define reproductive performance using a national breeding index in Ireland. Cows had similar geneti ...
DOI:: 10.1071/RD15128
: https://doi.org/10.1071/RD15128

71. Differential proteomic analysis of platelets suggested target-related proteins in rabbit platelets treated with Rhizoma Corydalis

Author:: Chun-Hong Li; Cen Chen; Qian Zhang; Chen-Ning Tan; Yuan-Jia Hu; Peng Li; Jian-Bo Wan; Gang Feng; Zhi-Ning Xia; Feng-Qing Yang
Source:: Pharmaceutical biology 2017 v.55 no.1 pp. 76-87
ISSN:: 1744-5116
Subject:: Corydalis yanhusuo; adenosine diphosphate; arachidonic acid; blood platelets; calcium; cyclic GMP; cytoskeleton; enzyme-linked immunosorbent assay; ethanol; fluorescence; gene expression regulation; matrix-assisted laser desorption-ionization mass spectrometry; molecular chaperones; phosphatidylinositol 3-kinase; platelet aggregation; proteomics; rabbits; rhizomes; serotonin; signal transduction; thrombin; two-dimensional gel electrophoresis
Abstract:: ... Context: Corydalis yanhusuo W.T. Wang (Papaveraceae) (Rhizoma Corydalis) showed inhibitory effects on rabbit platelet aggregation induced by ADP, thrombin (THR) or arachidonic acid (AA). Objective: This study separates and identifies the possible target-related platelet proteins and suggests possible signal cascades of RC antiplatelet aggregation. Materials and methods: Based on comparative proteo ...
DOI:: 10.1080/13880209.2016.1229340
PubMed:: 27653279
PubMed Central:: PMC7011957
: https://doi.org/10.1080/13880209.2016.1229340

72. Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

Author:: Patrícia Rosa de Oliveira; Luis Adriano Anholeto; Gerváso Henrique Bechara; Maria Izabel Camargo Mathias
Source:: Acta tropica 2017 v.166 pp. 139-154
ISSN:: 0001-706X
Subject:: Rhipicephalus sanguineus; acaricides; active ingredients; biochemical oxygen demand; cytoskeleton; death; defense mechanisms; dinotefuran; egg yolk; endosomes; female fertility; females; lipids; microvilli; midgut; myelin sheath; nutrition; plasma membrane; ticks; vacuoles; vitellogenesis
Abstract:: ... The present study demonstrated the effects of dinotefuran (active ingredient of the acaricide Protetor Pet®) on the ovary and midgut cells of semi engorged R. sanguineus females exposed to different concentrations of this chemical. For this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000ppm), groups I ...
DOI:: 10.1016/j.actatropica.2016.11.017
: https://doi.org/10.1016/j.actatropica.2016.11.017

73. Down-regulation of PAX2 promotes in vitro differentiation of podocytes from human CD34+ cells

Author:: Manne Mudhu Sunitha; Lokanathan Srikanth; Pasupuleti Santhosh Kumar; Chodimella Chandrasekhar; Potukuchi Venkata Gurunadha Krishna Sarma
Source:: Cell and tissue research 2017 v.370 no.3 pp. 477-488
ISSN:: 0302-766X
Subject:: adults; agarose; blood; cell movement; cytoskeleton; diaphragm; enzyme activity; glomerular filtration rate; glycoproteins; humans; immunocytochemistry; kidney cells; kidney diseases; monoclonal antibodies; phosphorylation; retinoic acid; staining; stem cells; tyrosine
Abstract:: ... Podocytes are major kidney cells that help in glomerular filtration and any damage or loss is a major event in the progression of kidney diseases. Understanding podocytes development will help in designing therapeutic strategies against these renal diseases. Therefore, in vitro generation of podocytes from adult hematopoietic CD34⁺ stem cells is explored in the present study. Apheretically, isolat ...
DOI:: 10.1007/s00441-017-2680-2
: https://doi.org/10.1007/s00441-017-2680-2

74. Drinking problems: mechanisms of macropinosome formation and maturation

Author:: Catherine M. Buckley; Jason S. King
Source:: FEBS journal 2017 v.284 no.22 pp. 3778-3790
ISSN:: 1742-464X
Subject:: atherosclerosis; cytoskeleton; dendritic cells; digestion; drinking; macrophages; neoplasms; neurodegenerative diseases; neurons; nutrients; pathogens; plasma membrane
Abstract:: ... Macropinocytosis is a mechanism for the nonspecific bulk uptake and internalisation of extracellular fluid. This plays specific and distinct roles in diverse cell types such as macrophages, dendritic cells and neurons, by allowing cells to sample their environment, extract extracellular nutrients and regulate plasma membrane turnover. Macropinocytosis has recently been implicated in several diseas ...
DOI:: 10.1111/febs.14115
: https://doi.org/10.1111/febs.14115

75. Drosophila tensin plays an essential role in cell migration and planar polarity formation during oogenesis by mediating integrin-dependent extracellular signals to actin organization

Author:: In Jun Cha; Jang Ho Lee; Kyoung Sang Cho; Sung Bae Lee
Source:: Biochemical and biophysical research communications 2017 pp. -
ISSN:: 0006-291X
Subject:: Drosophila; actin; animal ovaries; cell movement; cytoskeleton; eggs; hatching; integrins; mutants; mutation; oogenesis; phenotype; receptors
Abstract:: ... Oogenesis in Drosophila involves very dynamic cellular changes such as cell migration and polarity formation inside an ovary during short period. Previous studies identified a number of membrane-bound receptors directly receiving certain types of extracellular inputs as well as intracellular signalings to be involved in the regulation of these dynamic cellular changes. However, yet our understandi ...
DOI:: 10.1016/j.bbrc.2017.01.183
: https://doi.org/10.1016/j.bbrc.2017.01.183

76. Dusky works upstream of Four‐jointed and Forked in wing morphogenesis in Tribolium castaneum

Author:: C. Li; B. Li; S. Ma; P. Lü; K. Chen
Source:: Insect molecular biology 2017 v.26 no.6 pp. 677-686
ISSN:: 0962-1075
Subject:: Drosophila melanogaster; RNA interference; Tribolium castaneum; adults; cytoskeleton; elytra; fat body; genes; head; larvae; morphogenesis
Abstract:: ... Dusky (dy) is required for cytoskeletal reorganization during wing morphogenesis in Drosophila melanogaster, but which genes participate together with dy for wing morphogenesis has remained unclear. In Tribolium castaneum, dy is highly expressed at the late embryonic stage. Tissue‐specific expression analysis indicated high expression levels of dy in the epidermis, head and fat body of late‐stage ...
DOI:: 10.1111/imb.12327
: https://doi.org/10.1111/imb.12327

77. Dynamic regulation of small RNAome during the early stage of cardiac differentiation from pluripotent embryonic stem cells

Author:: Yue Li; An Zeng; Ge Li; Ya-Na Guan; Huang-Tian Yang; Bairong Shen; Qing Jing
Source:: Genomics data 2017 pp. -
ISSN:: 2213-5960
Subject:: high-throughput nucleotide sequencing; models; transfer RNA; cardiomyocytes; cytoskeleton; mice; microRNA; embryonic stem cells; cell differentiation; gene expression regulation; small interfering RNA
Abstract:: ... Embryonic stem cells (mESCs), having potential to differentiate into three germ-layer cells including cardiomyocytes, shall be a perfect model to help understanding heart development. Here, using small RNA deep sequencing, we studied the small RNAome in the early stage of mouse cardiac differentiation. We found that the expression pattern of most microRNA (miRNA) were highly enriched at the beginn ...
DOI:: 10.1016/j.gdata.2017.05.006
PubMed:: 28540181
PubMed Central:: PMC5432660
: https://doi.org/10.1016/j.gdata.2017.05.006

78. EZH2-mediated α-actin methylation needs lncRNA TUG1, and promotes the cortex cytoskeleton formation in VSMCs

Author:: Rong Chen; Peng Kong; Fan Zhang; Ya-Nan Shu; Xi Nie; Li-Hua Dong; Yan-Ling Lin; Xiao-Li Xie; Li-Li Zhao; Xiang-jian Zhang; Mei Han
Source:: Gene 2017 v.616 pp. 52-57
ISSN:: 0378-1119
Subject:: actin; binding proteins; cortex; cytoskeleton; depolymerization; gene expression regulation; genes; homeostasis; methylation; methyltransferases; myocytes; non-coding RNA; nucleotide sequences; polymerization; quantitative polymerase chain reaction; rats; smooth muscle; taurine
Abstract:: ... Recent studies have revealed that long non-coding RNAs (lncRNAs) participate in vascular homeostasis and pathophysiological conditions development. But still very few literatures elucidate the regulatory mechanism of non-coding RNAs in this biological process. Here we identified lncRNA taurine up-regulated gene 1 (TUG1) in rat vascular smooth muscle cells (VSMCs), and got 4612bp nucleotide sequenc ...
DOI:: 10.1016/j.gene.2017.03.028
: https://doi.org/10.1016/j.gene.2017.03.028

79. Ecotoxicoproteomic assessment of the functional alterations caused by chronic metallic exposures in gammarids

Author:: E. Gismondi; J.-P. Thomé; N. Urien; E. Uher; D. Baiwir; G. Mazzucchelli; E. De Pauw; L.C. Fechner; J.D. Lebrun
Source:: Environmental pollution 2017 pp. -
ISSN:: 0269-7491
Subject:: Gammarus fossarum; acclimation; aquatic organisms; biomarkers; cadmium; cecum; chronic exposure; copper; cytoskeleton; ecotoxicology; energy transfer; immune response; lead; males; molting; mortality; osmoregulation; oxidative stress; population dynamics; protein synthesis; proteins; proteomics; reproduction; toxicity
Abstract:: ... Very few ecotoxicological studies have been performed on long-term exposure under controlled conditions, hence limiting the assessment of the impact of chronic and diffuse chemical pressures on the health of aquatic organisms. In this study, an ecotoxicoproteomic approach was used to assess the integrated response and possible acclimation mechanisms in Gammarus fossarum following chronic exposures ...
DOI:: 10.1016/j.envpol.2017.03.006
: https://doi.org/10.1016/j.envpol.2017.03.006

80. Effect of liquid helium vitrification on cytoskeleton of immature cattle oocytes

Author:: Xian Fei Guo; Xue Li Yu; Fan Zhang; Hua Wu; Xu Zhe Pei; Xiao Xia Li; Ying Hua Li
Source:: Animal reproduction science 2017 v.187 pp. 91-99
ISSN:: 0378-4320
Subject:: blastocyst; cattle; cytoskeleton; helium; liquids; nitrogen; oocytes; viability; vitrification
Abstract:: ... The developmental potential and the changes in cytoskeleton structures in immature oocytes of cattle resulting from liquid helium (LHe) vitrification was evaluated in this study. Immature oocytes were randomly divided into three groups: fresh oocytes (negative control), oocytes vitrified in liquid nitrogen (LN group, positive control), and oocytes vitrified in LHe (LHe group). In Experiment 1, the ...
DOI:: 10.1016/j.anireprosci.2017.10.010
: https://doi.org/10.1016/j.anireprosci.2017.10.010

81. Effect of nano‐sized, elemental selenium supplement on the proteome of chicken liver

Author:: G. Gulyas; E. Csosz; J. Prokisch; A. Javor; M. Mezes; M. Erdelyi; K. Balogh; T. Janaky; Z. Szabo; A. Simon; L. Czegledi
Source:: Journal of animal physiology and animal nutrition 2017 v.101 no.3 pp. 502-510
ISSN:: 0931-2439
Subject:: actin; antioxidants; bioavailability; broiler chickens; carbohydrates; cytoskeleton; digestion; fatty acid metabolism; feed supplements; liquid chromatography; liver; mass spectrometry; protein synthesis; proteome; regulatory proteins; selenium; toxicity; two-dimensional gel electrophoresis
Abstract:: ... The nano‐sized (100–500 nm) selenium has higher bioavailability and relatively lower toxicity compared to other selenium forms. The objective of the present study was to compare liver proteome profiles of broiler chicken fed with control diet without Se supplementation and diet supplemented with nano‐Se with 4.25 mg/kg DM. Differential proteome analyses were performed by two‐dimensional gel electr ...
DOI:: 10.1111/jpn.12459
: https://doi.org/10.1111/jpn.12459

82. Effect of vitrification on the mRNA transcriptome of bovine oocytes

Author:: N Wang; C‐Y Li; H‐B Zhu; H‐S Hao; H‐Y Wang; C‐L Yan; S‐J Zhao; W‐H Du; D Wang; Y Liu; Y‐W Pang; X‐M Zhao
Source:: Reproduction in domestic animals 2017 v.52 no.4 pp. 531-541
ISSN:: 0936-6768
Subject:: calcium; cattle; cell cycle; cytoskeleton; gene expression; gene expression regulation; gene ontology; genes; glucose; ions; messenger RNA; metabolism; mitochondria; oocytes; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ribosomal proteins; sequence analysis; transcription (genetics); transcriptome; transmembrane proteins; vitrification
Abstract:: ... Vitrification has been shown to decrease the developmental capacity of mammalian oocytes, and this is closely associated with the abnormal mRNA expressions of vitrified oocytes. However, the effect of vitrification on transcriptional machinery of oocytes examined by RNA sequencing (RNA‐seq) has yet to be defined. In the present study, the mRNA transcriptomes of fresh and vitrified bovine oocytes w ...
DOI:: 10.1111/rda.12942
: https://doi.org/10.1111/rda.12942

83. Effectiveness of human spermatozoa biomarkers as indicators of structural damage during cryopreservation

Author:: María José Gómez-Torres; Llanos Medrano; Alejandro Romero; Pedro José Fernández-Colom; Jon Aizpurúa
Source:: Cryobiology 2017 v.78 pp. 90-94
ISSN:: 0011-2240
Subject:: DNA; DNA fragmentation; acrosome; acrosome reaction; biomarkers; cryopreservation; cryoprotectants; cytoskeleton; freeze-thaw cycles; humans; male fertility; metabolism; principal component analysis; semen; thawing
Abstract:: ... Human spermatozoa cryopreservation techniques are used to maintain and protect male fertility in cases such as infertility and malignancy treatments. However, during cryopreservation, the spermatozoa's metabolic rate is reduced and they undergo dramatic functional and structural changes owing to exposure to cryoprotectants and freezing-thawing procedures. While the effects of cryopreservation on c ...
DOI:: 10.1016/j.cryobiol.2017.06.008
: https://doi.org/10.1016/j.cryobiol.2017.06.008

84. Effects of ovarian disaggregation on adult murine follicle yield and viability

Author:: Fiona Young; John Drummond; Emma Akers; Louise Bartle; David Kennedy; Mohammad Asaduzzaman
Source:: Reproduction, fertility, and development 2017 v.29 no.12 pp. 2400-2410
ISSN:: 1031-3613
Subject:: 4',6-diamidino-2-phenylindole; adults; antibodies; collagenase; cytoskeleton; fluorescein; genetic markers; granulosa cells; mice; mitochondria; rapid methods; screening; staining; tubulin; viability
Abstract:: ... Follicles are isolated from ovaries for numerous reasons, including IVM, but adult murine yields are <2 folliclesmg-1. The aim of the present study was to optimise ovarian disaggregation and develop methods applicable to the rapid screening of follicle viability. Ovaries from adult mice (n=7) were halved and disaggregated mechanically, or by using collagenase IV (Col-IV; 590UmL-1) or animal origin ...
DOI:: 10.1071/RD16398
: https://doi.org/10.1071/RD16398

85. Effects of vitrification of cumulus-enclosed porcine oocytes at the germinal vesicle stage on cumulus expansion, nuclear progression and cytoplasmic maturation

Author:: Ruth Appeltant; Tamás Somfai; Elisa C. S. Santos; Thanh Quang Dang-Nguyen; Takashi Nagai; Kazuhiro Kikuchi
Source:: Reproduction, fertility, and development 2017 v.29 no.12 pp. 2419-2429
ISSN:: 1031-3613
Subject:: actin; adenosine triphosphate; apoptosis; cell nucleolus; cooling; cryoprotectants; embryogenesis; ethylene glycol; germinal vesicle; glutathione; meiosis; messenger RNA; progeny; propylene glycol; swine; toxicity; vitrification
Abstract:: ... Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus–oocyte complexes (COCs) and control COCs (no cryoprotectant treatment ...
DOI:: 10.1071/RD16386
: https://doi.org/10.1071/RD16386

86. Electrically conductive hydrogel-based micro-topographies for the development of organized cardiac tissues

Author:: Ali Navaei; Nathan Moore; Ryan T. Sullivan; Danh Truong; Raymond Q. Migrino; Mehdi Nikkhah
Source:: RSC advances 2017 v.7 no.6 pp. 3302-3312
ISSN:: 2046-2069
Subject:: actin; biocompatible materials; biomimetics; connexins; cytoskeleton; electrical properties; fluorescence; gelatin; gold; hydrogels; myocardial infarction; myocardium; nanogold; nanorods; tissue engineering
Abstract:: ... The dense uniaxially aligned cardiac cytoarchitecture of the myocardium along with electrical and mechanical coupling between the cardiac cells are key factors in the synchronous contractility of the heart. Following myocardial infarction, the organized architecture of the myocardium is disrupted and non-functional scar tissues are produced. Engineered cardiac tissues, developed in hydrogel-based ...
DOI:: 10.1039/c6ra26279a
: https://doi.org/10.1039/c6ra26279a

87. Electrospun poly(ethylene oxide)/chitosan nanofibers with cellulose nanocrystals as support for cell culture of 3T3 fibroblasts

Author:: Daniela M. Ridolfi; Ana Paula Lemes; Sheila de Oliveira; Giselle Z. Justo; Marcelly V. Palladino; Nelson Durán
Source:: Cellulose 2017 v.24 no.8 pp. 3353-3365
ISSN:: 0969-0239
Subject:: actin; cell culture; cellulose; chitosan; cytoskeleton; fibroblasts; nanocrystals; nanofibers; polyethylene glycol; thermogravimetry; tubulin
Abstract:: ... Chitosan/poly(ethylene oxide) (PEO) (5:1) nanofibers with cellulose nanocrystals (CNCs) were produced using an electrospinning technique. The addition of CNCs to the chitosan/PEO solutions allowed the production of uniform fibers (without beads) with a high proportion of chitosan. The fiber diameters were influenced by the concentration of CNCs in the chitosan/PEO solutions. The solutions containi ...
DOI:: 10.1007/s10570-017-1362-2
: https://doi.org/10.1007/s10570-017-1362-2

88. Endocytosis and its inhibitors in basidiomycetous fungus Rhizoctonia solani

Author:: O. V. Kamzolkina; M. A. Kiselica; O. A. Kudryavtseva; O. V. Shtaer; I. S. Mazheika
Source:: Moscow University biological sciences bulletin 2017 v.72 no.3 pp. 128-136
ISSN:: 0096-3925
Subject:: Thanatephorus cucumeris; absorption; actin; cell membranes; cytoskeleton; endocytosis; fungi; humans; microparticles; mycelium; mycology; tubulin
Abstract:: ... Endocytosis is a complex process of absorption from the environment (and subsequent distribution within the cell) of soluble substances, macromolecules, microparticles, etc. by means of vesicles developed by cytoplasmic membrane. Endocytosis in animal and human cells is actively and successfully studied. Thus, classification of this process in the animals (based only on the peculiarities of primar ...
DOI:: 10.3103/S0096392517030063
: https://doi.org/10.3103/S0096392517030063

89. Energy Dissipation Mapping of Cancer Cells

Author:: Diganta Dutta; Xavier-Lewis Palmer; Jinhyun Kim; Shizhi Qian; Michael Stacey
Source:: Micron 2017 pp. -
ISSN:: 0968-4328
Subject:: atomic force microscopy; cytoskeleton; electric field; energy; monitoring; neoplasm cells; silica; topography
Abstract:: ... The purpose of this study is to map the energy dissipation of Jurkat cells using a single 60 nanosecond pulse electric field (NsPEF), primarily through atomic force microscopy (AFM). The phase shift is generated by the sample elements that do not have a heterogeneous surface. Monitoring and manipulating the phase shift is a powerful way for determining the dissipated energy and plotting the topogr ...
DOI:: 10.1016/j.micron.2017.11.005
: https://doi.org/10.1016/j.micron.2017.11.005

90. Enhanced directional cell migration induced by vaccinia virus on a microfluidic-based multi-shear cell migration assay platform

Author:: Cheng Wang; Na Xu; Yu-Jun Yang; Qiu-Mei Wu; Dai-Wen Pang; Zhi-Ling Zhang
Source:: Integrative biology 2017 v.9 no.12 pp. 903-911
ISSN:: 1757-9708
Subject:: Golgi apparatus; Vaccinia virus; cell movement; cytoskeleton; organ-on-a-chip; shear stress; virion; viruses
Abstract:: ... Virus-induced cell migration plays important roles in the direct and rapid spread of virus particles. As cell migration is also regulated by shear stress, it is necessary to explore the cell migration behavior influenced by multiple factors including a virus and shear stress. In this report, a three-layer microfluidic chip with symmetric channels was designed and fabricated to investigate vaccinia ...
DOI:: 10.1039/c7ib00151g
: https://doi.org/10.1039/c7ib00151g

91. Enteric glial cells are susceptible to Clostridium difficile toxin B

Author:: Katia Fettucciari; Pamela Ponsini; Davide Gioè; Lara Macchioni; Camilla Palumbo; Elisabetta Antonelli; Stefano Coaccioli; Vincenzo Villanacci; Lanfranco Corazzi; Pierfrancesco Marconi; Gabrio Bassotti
Source:: Cellular and molecular life sciences 2017 v.74 no.8 pp. 1527-1551
ISSN:: 1420-682X
Subject:: Clostridium difficile; apoptosis; cell cycle checkpoints; colitis; cyclins; cytoskeleton; cytotoxicity; diarrhea; enterocytes; glucosylation; guanosinetriphosphatase; homeostasis; inflammation; interferon-gamma; neuroglia; neurons; pathogenesis; toxins; tumor necrosis factor-alpha; virulence
Abstract:: ... Clostridium difficile causes nosocomial/antibiotic-associated diarrhoea and pseudomembranous colitis. The major virulence factors are toxin A and toxin B (TcdB), which inactivate GTPases by monoglucosylation, leading to cytopathic (cytoskeleton alteration, cell rounding) and cytotoxic effects (cell-cycle arrest, apoptosis). C. difficile toxins breaching the intestinal epithelial barrier can act on ...
DOI:: 10.1007/s00018-016-2426-4
: https://doi.org/10.1007/s00018-016-2426-4

92. Enterolactone alters FAK-Src signaling and suppresses migration and invasion of lung cancer cell lines

Author:: Shireen Chikara; Kaitlin Lindsey; Pawel Borowicz; Melpo Christofidou-Solomidou; Katie M. Reindl
Source:: BMC complementary and alternative medicine 2017 v.17 no.1 pp. 30
ISSN:: 1472-6882
Subject:: Western blotting; actin; alternative medicine; antineoplastic activity; bioactive compounds; bromides; cell movement; cytoskeleton; drug therapy; fluorescence microscopy; focal adhesions; gene expression; genes; humans; immunocytochemistry; lignans; lung neoplasms; messenger RNA; metastasis; neoplasm cells; phosphorylation; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; tissue repair; tissues; toxicity
Abstract:: ... BACKGROUND: Systemic toxicity of chemotherapeutic agents and the challenges associated with targeting metastatic tumors are limiting factors for current lung cancer therapeutic approaches. To address these issues, plant-derived bioactive components have been investigated for their anti-cancer properties because many of these agents are non-toxic to healthy tissues. Enterolactone (EL) is a flaxseed ...
DOI:: 10.1186/s12906-016-1512-3
PubMed:: 28068967
PubMed Central:: PMC5223372
: https://doi.org/10.1186/s12906-016-1512-3

93. Epigallocatechin-3-gallate reduces the proliferation of benign prostatic hyperplasia cells via regulation of focal adhesions

Author:: Burcu Erbaykent Tepedelen; Elif Soya; Mehmet Korkmaz
Source:: Life sciences 2017 v.191 pp. 74-81
ISSN:: 0024-3205
Subject:: actin; adverse effects; cell division; cell movement; cell proliferation; cytoskeleton; dose response; epigallocatechin gallate; epithelium; fluorescent antibody technique; focal adhesions; genes; hyperplasia; immunoblotting; inflammation; non-specific protein-tyrosine kinase; oxidative stress; stromal cells; therapeutics; tissue repair
Abstract:: ... Benign prostatic hyperplasia (BPH) is the most common urological disease that is characterized by the excessive growth of prostatic epithelial and stromal cells. Pharmacological therapy for BPH has limited use due to the many side effects so there is a need for new agents including natural compounds such as epigallocatechin-3-gallate (EGCG). This study was undertaken to assess the role of EGCG, su ...
DOI:: 10.1016/j.lfs.2017.10.016
: https://doi.org/10.1016/j.lfs.2017.10.016

94. Establishment of a de novo Reference Transcriptome of Histomonas meleagridis Reveals Basic Insights About Biological Functions and Potential Pathogenic Mechanisms of the Parasite

Author:: Rounik Mazumdar; Lukas Endler; Andreas Monoyios; Michael Hess; Ivana Bilic
Source:: Protist 2017 v.168 no.6 pp. 663-685
ISSN:: 1434-4610
Subject:: Histomonas meleagridis; cDNA libraries; chickens; complementary DNA; cytoskeleton; diagnostic techniques; drugs; gene ontology; genomics; metabolism; molecular biology; mortality; parasites; transcriptome; transcriptomics; turkeys; virulence
Abstract:: ... The protozoan flagellate Histomonas meleagridis is the causative agent of histomonosis in poultry. In turkeys, high mortality might be noticed whereas in chickens the disease is less severe despite production losses. Discovered over a century ago, molecular data on this parasite are scarce and genetic studies are in its infancy. To expand genomic information, a de novo transcriptome sequencing of ...
DOI:: 10.1016/j.protis.2017.09.004
: https://doi.org/10.1016/j.protis.2017.09.004

95. Eukaryotic origins and the Proterozoic Earth system: A link between global scale glaciations and eukaryogenesis?

Author:: Richard Boyle
Source:: Earth-science reviews 2017 pp. -
ISSN:: 0012-8252
Subject:: DNA; Earth system science; Gibbs free energy; adenosine triphosphate; cell membranes; cytoskeleton; eukaryotic cells; fecundity; fossils; genes; glaciation; mitochondria; oceans; phagocytosis; phylogeny; prediction; prokaryotic cells; symbionts; symbiosis; transcription (genetics)
Abstract:: ... The Proterozoic Earth system is popularly viewed as having comprised prolonged periods of invariant conditions separating intervals of extreme change. Against this backdrop the earliest evidence of eukaryotic organisms is found, raising the (highly uncertain) possibility of an environmental impetus for this fundamental evolutionary transition. Here I review the eukaryotic fossil record and the the ...
DOI:: 10.1016/j.earscirev.2017.09.001
: https://doi.org/10.1016/j.earscirev.2017.09.001

96. Expansion and cryopreservation of porcine and human corneal endothelial cells

Author:: Leah A. Marquez-Curtis; Locksley E. McGann; Janet A.W. Elliott
Source:: Cryobiology 2017 v.77 pp. 1-13
ISSN:: 0011-2240
Subject:: actin; basement membrane; blindness; cooling; cornea; cryopreservation; cytoskeleton; dimethyl sulfoxide; endothelium; human umbilical vein endothelial cells; humans; medicine; nitrogen; starch; swine; thawing; tight junctions; tissue engineering
Abstract:: ... Impairment of the corneal endothelium causes blindness that afflicts millions worldwide and constitutes the most often cited indication for corneal transplants. The scarcity of donor corneas has prompted the alternative use of tissue-engineered grafts which requires the ex vivo expansion and cryopreservation of corneal endothelial cells. The aims of this study are to culture and identify the condi ...
DOI:: 10.1016/j.cryobiol.2017.04.012
: https://doi.org/10.1016/j.cryobiol.2017.04.012

97. Exploiting molecular motors as nanomachines: the mechanisms of de novo and re-engineered cytoskeletal motors

Author:: Nicole V DelRosso; Nathan D Derr
Source:: Current opinion in biotechnology 2017 v.46 pp. 20-26
ISSN:: 0958-1669
Subject:: adenosine triphosphate; cytoskeleton; energy; kinesin; nanotechnology; transporters
Abstract:: ... Cytoskeletal molecular motors provide exciting proof that nanoscale transporters can be highly efficient, moving for microns along filamentous tracks by hydrolyzing ATP to fuel nanometer-size steps. For nanotechnology, such conversion of chemical energy into productive work serves as an enticing platform for re-purposing and re-engineering. It also provides a roadmap for successful molecular mecha ...
DOI:: 10.1016/j.copbio.2016.10.011
: https://doi.org/10.1016/j.copbio.2016.10.011

98. Exploring the evolution of the proteins of the plant nuclear envelope

Author:: Axel Poulet; Aline V. Probst; Katja Graumann; Christophe Tatout; David Evans
Source:: Nucleus 2017 v.8 no.1 pp. 46-59
ISSN:: 1949-1042
Subject:: convergent evolution; cytoskeleton; models; nuclear membrane; plasticity
Abstract:: ... In this study, we explore the plasticity during evolution of proteins of the higher plant nuclear envelope (NE) from the most ancestral plant species to advanced angiosperms. The higher plant NE contains a functional Linker of Nucleoskeleton and Cytoskeleton (LINC) complex based on conserved Sad1-Unc84 (SUN) domain proteins and plant specific Klarsicht/Anc1/Syne homology (KASH) domain proteins. Re ...
DOI:: 10.1080/19491034.2016.1236166
PubMed:: 27644504
PubMed Central:: PMC5287204
: https://doi.org/10.1080/19491034.2016.1236166

99. Exposure of okadaic acid alters the angiogenesis in developing chick embryos

Author:: Yu-hu Jiao; Min Dou; Guang Wang; Hong-ye Li; Jie-sheng Liu; Xuesong Yang; Wei-dong Yang
Source:: Toxicon 2017 v.133 pp. 74-81
ISSN:: 0041-0101
Subject:: DNA damage; angiogenesis; apoptosis; ascorbic acid; blood vessels; chickens; chicks; chorioallantoic membrane; chromosome elimination; cytoskeleton; diarrhetic shellfish poisoning; embryotoxicity; humans; okadaic acid; phycotoxins; superoxide dismutase; tissues; yolk sac
Abstract:: ... Okadaic acid (OA) is a common phycotoxin, which concerns diarrheic shellfish poisoning (DSP) in human being. It has been known that OA can induce disorganization in cytoskeletal architecture and cell-cell contact, cause chromosome loss, apoptosis, DNA damage and inhibit phosphatases, suggesting its potential embryotoxicity. In this paper, we found that low concentration of OA (50 nM, 100 nM and 20 ...
DOI:: 10.1016/j.toxicon.2017.05.003
: https://doi.org/10.1016/j.toxicon.2017.05.003

100. Exposure to lethal levels of benzo[a]pyrene or cadmium trigger distinct protein expression patterns in earthworms (Eisenia fetida)

Author:: Lihao Zhang; Xiaochen Duan; Nannan He; Xu Chen; Jinli Shi; Weiming Li; Li Xu; Huixin Li
Source:: Science of the total environment 2017 pp. -
ISSN:: 0048-9697
Subject:: Eisenia fetida; analysis of variance; benzo(a)pyrene; cadmium; calcium; catalase; cytoskeleton; earthworms; gene expression; gene expression regulation; genes; genotoxicity; heat-shock protein 70; messenger RNA; metallothionein; myosin; oxidative stress; pollutants; protein composition; protein synthesis; proteomics; quantitative polymerase chain reaction; reactive oxygen species; soil; China
Abstract:: ... Different pollutants induce distinct toxic responses in earthworms (Eisenia fetida). Here, we used proteomics techniques to compare the responses of E. fetida to exposure to the 10% lethal concentration (14d-LC10) of benzo[a]pyrene (BaP) or cadmium (Cd) in natural red soil (China). BaP exposure markedly induced the expression of oxidation-reduction proteins, whereas Cd exposure mainly induced the ...
DOI:: 10.1016/j.scitotenv.2017.04.003
: https://doi.org/10.1016/j.scitotenv.2017.04.003

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1. 2,3,7,8-Tetrachlorodibenzo-p-dioxin influences bovine herpesvirus 1 replication through upregulation of SIRT3 and cytoskeletal reorganization

2. A Cyclin D2-derived peptide acts on specific cell cycle phases by activating ERK1/2 to cause the death of breast cancer cells

3. A Noncanonical Binding Site in the EVH1 Domain of Vasodilator-Stimulated Phosphoprotein Regulates Its Interactions with the Proline Rich Region of Zyxin

4. A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis

5. A Shotgun Proteomics Approach Reveals a New Toxic Role for Alzheimer’s Disease Aβ Peptide: Spliceosome Impairment

6. A genome‐wide association study of heat stress‐associated SNPs in catfish

7. A high-throughput QCM chip configuration for the study of living cells and cell-drug interactions

8. A novel ex vivo immunoproteomic approach characterising Fasciola hepatica tegumental antigens identified using immune antibody from resistant sheep

9. A novel microfluidic device to model the human proximal tubule and glomerulus

10. A proteomic approach to assess the host response in gills of farmed Atlantic salmon Salmo salar L. affected by amoebic gill disease

11. ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

12. Acoustic tweezing cytometry enhances osteogenesis of human mesenchymal stem cells through cytoskeletal contractility and YAP activation

13. Activated microglia trigger inflammasome activation and lysosomal destabilization in human RPE cells

14. Adhesion of bone marrow mesenchymal stem cells on porous titanium surfaces with strontium-doped hydroxyapatite coating

15. Adhesion profile and differentiation capacity of human adipose tissue derived mesenchymal stem cells grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coated surfaces

16. Aeromonas salmonicida type III secretion system-effectors-mediated immune suppression in rainbow trout (Oncorhynchus mykiss)

17. Aerosol from Tobacco Heating System 2.2 has reduced impact on mouse heart gene expression compared with cigarette smoke

18. Analysis of differentially accumulated proteins associated with graft union formation in pecan (Carya illinoensis)

19. Analysis of the spleen proteome of chickens infected with reticuloendotheliosis virus

20. Andrographolide disrupts meiotic maturation by blocking cytoskeletal reorganisation and decreases the fertilisation potential of mouse oocytes

21. Anti-inflammatory effects of the selective phosphodiesterase 3 inhibitor, cilostazol, and antioxidants, enzymatically-modified isoquercitrin and α-lipoic acid, reduce dextran sulphate sodium-induced colorectal mucosal injury in mice

22. Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows

23. Automated three-dimensional reconstruction of the Caenorhabditis elegans germline

24. Bacteria and endothelial cells: a toxic relationship

25. Betanodavirus-like particles enter host cells via clathrin-mediated endocytosis in a cholesterol-, pH- and cytoskeleton-dependent manner

26. Bioinformatic search for Ca2+- and calmodulin-dependent protein kinases potentially associated with the regulation of plant cytoskeleton

27. Biological Responses and Mechanisms of Human Bone Marrow Mesenchymal Stem Cells to Zn and Mg Biomaterials

28. Blastocyst hatching in humans

29. Blebbistatin induces chondrogenesis of single mesenchymal cells via PI3K/PDK1/mTOR/p70S6K pathway

30. Blood-brain barrier dysfunction induced by silica NPs in vitro and in vivo: Involvement of oxidative stress and Rho-kinase/JNK signaling pathways

31. Brain proteomic modifications associated to protective effect of grape extract in a murine model of obesity

32. Buyang Huanwu decoction facilitates neurorehabilitation through an improvement of synaptic plasticity in cerebral ischemic rats

33. Calpain inhibition prevents flotillin re-ordering and Src family activation during capacitation

34. Candida albicans Modifies the Protein Composition and Size Distribution of THP-1 Macrophage-Derived Extracellular Vesicles

35. Canonical and cellular pathways timing gamete release in Acropora digitifera, Okinawa, Japan

36. Carbon black aggregates cause endothelial dysfunction by activating ROCK

37. Cell polarity in plants: the Yin and Yang of cellular functions

38. Cell wall accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated Arabidopsis leaf epidermal cells

39. Cellular and molecular characterizations of a slow-growth variant provide insights into the fast growth of bamboo

40. Cerium oxide nanoparticles (CeO2 NPs) improve the developmental competence of in vitro-matured prepubertal ovine oocytes

41. Changes in the cerebellar cytoarchitecture of hibernating hedgehog Erinaceus europaeus L. (Mammalia): an immunocytochemical approach

42. Characterization of the Molecular Mechanisms Underlying the Chronic Phase of Stroke in a Cynomolgus Monkey Model of Induced Cerebral Ischemia

43. Cleavage of β-dystroglycan occurs in sarcoglycan-deficient skeletal muscle without MMP-2 and MMP-9

44. Clinical and histopathological features of myofibrillar myopathy in Warmblood horses

45. Cloning, characterization and subcellular localization of Nuclear LIM interactor interacting factor gene from Leishmania donovani

46. Co-Delivery of Trichosanthin and Albendazole by Nano-Self-Assembly for Overcoming Tumor Multidrug-Resistance and Metastasis

47. Coenzyme Q10 supplementation during in vitro maturation of bovine oocytes (Bos taurus) helps to preserve oocyte integrity after vitrification

48. Comparative proteomic analysis provides insight into 10-hydroxy-2-decenoic acid biosynthesis in honey bee workers

49. Comparative proteomics reveals the physiological differences between winter tender shoots and spring tender shoots of a novel tea (Camellia sinensis L.) cultivar evergrowing in winter

50. Comprehensive evaluation of gene expression signatures in response to electroacupuncture stimulation at Zusanli (ST36) acupoint by transcriptomic analysis

51. Contributions of polyunsaturated fatty acids (PUFA) on cerebral neurobiology: an integrated omics approach with epigenomic focus

52. Control of nuclear organization by F-actin binding proteins

53. Controlling cell volume for efficient PHB production by Halomonas

54. Coordinate expression of pan-neuronal and functional signature genes in sympathetic neurons

55. Critical role of Keratin 1 in maintaining epithelial barrier and correlation of its down-regulation with the progression of inflammatory bowel disease

56. Crosstalk of cell polarity signaling pathways

57. Cryopreservation of bull semen is associated with carbonylation of sperm proteins

58. Curcumin alters the cytoskeleton and microtubule organization on trophozoites of Giardia lamblia

59. Curdione attenuates thrombin-induced human platelet activation: β1-tubulin as a potential therapeutic target

60. Cyclin-dependent kinase 5 controls vasculogenic mimicry formation in non-small cell lung cancer via the FAK-AKT signaling pathway

61. Cytoskeleton and nuclear lamina affection in recessive osteogenesis imperfecta: A functional proteomics perspective

62. Cytoskeleton reorganization/disorganization is a key feature of induced inaccessibility for defence to successive pathogen attacks

63. Cytotoxicity of thirteen South African macrofungal species against five cancer cell lines

64. Daboialectin, a C-type lectin from Russell's viper venom induces cytoskeletal damage and apoptosis in human lung cancer cells in vitro

65. Data on evolution of intrinsically disordered regions of the human kinome and contribution of FAK1 IDRs to cytoskeletal remodeling

66. Deciphering the BAR code of membrane modulators

67. Deciphering the protein–protein interaction network regulating hepatocellular carcinoma metastasis

68. Deep transcriptome profiling sheds light on key players in nucleus implantation induced immune response in the pearl oyster Pinctada martensii

69. Differential Phosphoproteomic Analysis of Recombinant Chinese Hamster Ovary Cells Following Temperature Shift

70. Differential gene expression in the endometrium reveals cytoskeletal and immunological genes in lactating dairy cows genetically divergent for fertility traits