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... 24-Epibrassinolide (EBL) is considered the most probable brassinosteroid (BR) candidate that could be used for practical application in agriculture. EBL-induced stress-protective properties were evaluated in in vitro-grown tomato (Solanum lycopersicum L.) varieties Pusa Ruby (susceptible to nematodes) and PNR-7 (resistant to nematodes) during nematode pathogenesis. Sterilized tomato seeds treated ...
... A new amide, pipertaiwanamide (1), has been isolated from the aerial part of Piper taiwanense, together with four known compounds, taiwanamide A (2), taiwanamide B (3), 1-cinnamoylpyrrolidine (4), and N-cinnamoyltyramine (5). The structure of the new compound 1 was determined through spectroscopic and MS analyses. Pipertaiwanamide (1) and N-cinnamoyltyramine (5) exhibited free radical scavenging a ...
... A new flavonoid glycoside 1, along with seven known compounds (2–8), was isolated from Viburnum macrocephalum f. keteleeri by repeated column chromatography. Its structure was identified as apigenin-7-O-[6-O-(5-methoxy-3-hydroxy-3-methyl-5-oxovaleryl)]-β-D-glucopyranoside (1) by spectral analysis. ...
Cyclea; chemical structure; glucosides; lignans; spectral analysis
Abstract:
... A new lignan glucoside 1, along with two known lignans (2, 3), was isolated from Cyclea racemosa Oliv. Its structure was elucidated as (+)-7′-methoxylariciresinol 4-O-β-D-glucopyranoside on the basis of extensive spectroscopic analyses. ...
Aspergillus; Candida albicans; Staphylococcus aureus; antibacterial properties; mass spectrometry; nuclear magnetic resonance spectroscopy; spectral analysis
Abstract:
... Methyl (3-hydroxy-4-(2-hydroxy-6-methylheptan-2-yl)benzoyl)glycinate (1), a new sydonic acid derivative with glycinate, together with sydonic acid (2), sydowic acid (3), and 7-deoxy-7,14-didehydrosydonic acid (4), were isolated from a marine-derived Aspergillus sydowii strain CUGB-F126. Their structures were elucidated by spectroscopic analysis, including high-resolution mass spectroscopy, and 1D ...
Asteraceae; chemical structure; ethyl acetate; nuclear magnetic resonance spectroscopy; plant extracts; triterpenoids
Abstract:
... A new triterpenoid, namely 3β-(2-methoxy-5-formylphenoxy)-erythrodiol (1), was isolated from the EtOAc extract of Ixeris chinensis. The structures of 1 was elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS, and extensive 1D and 2D NMR techniques. ...
... An efficient transition-metal-free cascade reaction has been developed for the facile synthesis of 12H-benzo[4,5]thiazolo[2,3-b]quinazolin-12-one derivatives from commercially available isatins and 2-haloaryl isothiocyanates. A preliminary mechanistic study suggested an interrupted Dimroth rearrangement was the key step for the successful transformation. ...
... The inadequate response of Indica rice (Oryza sativa L.) to anther culture hinders the utility of this technique for rice crop improvement. This study focused on intensifying the in vitro anther response of selected varieties from Sri Lankan germplasm by precisely targeting the most effective maturity stage of microspores for culture. Analysis of five varieties responsive to anther culture (“At 30 ...
Human immunodeficiency virus 1; chemical compounds; chemical reactions; peptides
Abstract:
... A nanomotor based strategy for fast cellular entry and cargo delivery is presented. The concept focuses on integrating tat peptide, a basic domain of HIV-1 tat protein, with state of the art nanomotors which possess attractive autonomous properties, facilitating cellular penetration and uptake. The rapid cellular internalization process leads to higher delivery efficiency. ...
... Canine parvovirus (CPV) is an important and highly prevalent pathogen of dogs that causes acute hemorrhagic enteritis disease. Here, we describe a rapid method for the construction and characterization of a full-length infectious clone (rCPV) of CPV. Feline kidney (F81) cells were transfected with rCPV incorporating an engineered EcoR I site that served as a genetic marker. The rescued virus was i ...
animals; chemical compounds; microRNA; polymerization
Abstract:
... Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2′-O-methylated at the 3′ end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3′-terminal 2′-O-meth ...
carbonates; chemical reactions; ethylene; ligands; sulfates
Abstract:
... Novel multimetallic copper pyrazolate complexes, inaccessible using simple pyrazole ligands due to competing, alternative structural motifs, can be obtained by locking pairs of pyrazole ligands together with ethylene tethers. Nanojars based on this tethered pyrazole ligand display unexpected total selectivity for the carbonate over the sulfate ion. ...
... Changes in the activity of Na⁺/K⁺-ATPase and content of membrane lipids (phospholipids) in the gills and hepatopancreas of the blue mussel Mytilus edulis L. have been studied during a sharp temperature increase under aquarian managed conditions. The most pronounced changes were recorded in mollusk gills. A correlation of changes in the activity of membrane-bound Na⁺/K⁺-ATPase and phospholipid cont ...
Dianthus chinensis; antioxidant activity; enzyme activity; ethylene; guard cells; hydrogen peroxide; hyperhydricity; in vitro culture; in vitro studies; micropropagation; plantlets; silver nitrate; water content
Abstract:
... Hyperhydricity symptoms are common and significant during the in vitro culture of Dianthus chinensis L. and greatly affect the micropropagation and regeneration of cultured plantlets. However, effective measures for preventing such abnormalities have not been developed for this species. Silver nitrate (AgNO₃) has been shown to revert hyperhydric plantlets to a normal state. Nevertheless, the effec ...
borates; chemical reactions; nuclear magnetic resonance spectroscopy
Abstract:
... Allyl borates, a new class of monofunctional polyhomologation initiators, are reported. These monofunctional initiators are less sensitive and more effective towards polymethylene-based architectures. As an example, the synthesis of α-vinyl-ω-hydroxypolymethylenes is given. By designing/synthesizing different allylic borate initiators, and using ¹H and ¹¹B NMR spectroscopy, the initiation mechanis ...
Rafael R. Cantú; Celso Aita; Alexandre Doneda; Diego A. Giacomini; Alexandre Dessbesell; Marlon Arenhardt; Géssica G. De Bastiani; Stefen B. Pujol; Philippe Rochette; Martin H. Chantigny; Sandro J. Giacomini
... Alternative fertilization practices are needed for reducing gaseous and leaching N losses at high urea application rates. The objective of this study was to compare gaseous N emissions (N₂O and NH₃) and NO₃ ⁻ concentrations in the soil solution during two successive lettuce cropping seasons under contrasting fertilization practices. Treatments were fertilization with regular urea (U), urea treated ...
V. G. Pshennikova; N. A. Barashkov; A. V. Solovyev; G. P. Romanov; E. E. Diakonov; N. N. Sazonov; I. V. Morozov; A. A. Bondar; O. L. Posukh; L. U. Dzhemileva; E. K. Khusnutdinova; M. I. Tomsky; S. A. Fedorova
... Тhe DNA testing of autosomal recessive deafness type 1A (DFNB1A, MIM 220290) is complicated when deaf patients have only monoallelic (heterozygous) recessive mutations in the GJB2 (Сх26) gene that is uninformative for establishment of diagnosis. Such patients may be “random” heterozygous carriers of GJB2 mutations as well as have the mutant allele in a cis-regulatory region of GJB2 gene, in elemen ...
... Biliary atresia (BA) is a rare and serious liver disease in newborn infants. Previously, we reported that non-parenchymal cell (NPC) fractions from cirrhotic liver of BA may contain hepatic stem/progenitor cells in primary culture of NPC fractions. In this study, NPC fractions were subjected to primary or passage culture and found that clusters of hepatocyte-like cells appear even without adding h ...
... The emergence and disappearance of antigenic variants of foot-and-mouth disease virus (FMDV) during a field outbreak occurs periodically due to the volatile nature of its genome. In the present analysis, change in antigenic behavior of serotype O FMDV during the serial cytolytic passage in the absence of immune pressure was observed. Initially, the isolate showed a poor antigenic match (relationsh ...
... The ability of alkalophilic micromycetes of the Emericellopsis alkalinа to produce antimicrobial peptides has been studied. Evaluation of the spectrum and the yield of antibiotic compounds has allowed us to choose a promising producer of peptide antimycotics, Emericellopsis аlkalinа А118. The producer exhibits antifungal activity against conditionally pathogenic yeast and mold fungi, i.e., Candida ...