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You searched for: Publication year rev 7983-2017 Remove constraint Publication year rev: 7983-2017 Subject term DNA Remove constraint Subject term: DNA
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1. 2-Methoxystypandrone improves in vitro-produced bovine embryo quality through inhibition of IKBKB

Author:
Ayman Mesalam; Imran Khan; Kyeong-Lim Lee; Seok-Hwan Song; M.M.R. Chowdhury; Zia Uddin; Ki Hun Park; Il-Keun Kong
Source:
Theriogenology 2017 v.99 pp. 10-20
ISSN:
0093-691X
Subject:
DNA; Reynoutria japonica; antioxidant activity; antioxidants; apoptosis; blastocyst; cattle; embryogenesis; gene expression; genes; immunocytochemistry; in vitro culture; inducible nitric oxide synthase; medicinal properties; messenger RNA; non-specific protein-tyrosine kinase; oocytes; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; zygote
Abstract:
... 2-Methoxystypandrone (2-MS), a naphthoquinone from Polygonum cuspidatum, has been reported to exhibit antioxidant activities. However, its pharmacological properties have not been fully studied in vitro culture system of oocytes and embryos development. In this study, we examined the effects of 2-MS on bovine blastocyst developmental competency during preimplantation using TUNEL assay, qRT-PCR and ...
DOI:
10.1016/j.theriogenology.2017.05.012

2. 2D-IR Spectroscopy Shows that Optimized DNA Minor Groove Binding of Hoechst33258 Follows an Induced Fit Model

Author:
Ramakers Lennart A. I.; Hithell Gordon; May John J.; Greetham Gregory M.; Donaldson Paul M.; Towrie Michael; Parker Anthony W.; Burley Glenn A.; Hunt Neil T.
Source:
Journal of physical chemistry 2017 v.121 no.6 pp. 1295-1303
ISSN:
1520-5207
Subject:
DNA; chemical structure; hydrogen bonding; infrared spectroscopy; ligands; models
Abstract:
... The induced fit binding model describes a conformational change occurring when a small molecule binds to its biomacromolecular target. The result is enhanced noncovalent interactions between the ligand and biomolecule. Induced fit is well-established for small molecule–protein interactions, but its relevance to small molecule–DNA binding is less clear. We investigate the molecular determinants of ...
DOI:
10.1021/acs.jpcb.7b00345

3. 3-Hydroxy-4,7-megastigmadien-9-one, isolated from Ulva pertusa, attenuates TLR9-mediated inflammatory response by down-regulating mitogen-activated protein kinase and NF-κB pathways

Author:
Irshad Ali; Zahid Manzoor; Jung-Eun Koo; Jung-Eun Kim; Sang-Hee Byeon; Eun-Sook Yoo; Hee-Kyoung Kang; Jin-Won Hyun; Nam-Ho Lee; Young-Sang Koh
Source:
Pharmaceutical biology 2017 v.55 no.1 pp. 435-440
ISSN:
1744-5116
Subject:
DNA; IKappaB kinase; Ulva; Western blotting; anti-inflammatory activity; bioactive compounds; carotenoids; dendritic cells; enzyme-linked immunosorbent assay; human health; inflammation; inhibitory concentration 50; interleukin-12; interleukin-6; luciferase; macroalgae; mitogen-activated protein kinase; phosphorylation; phycocyanin; polyphenols; polysaccharides; reporter genes; transcription (genetics); transcription factor NF-kappa B; tumor necrosis factor-alpha; vitamins
Abstract:
... Context: Seaweeds are rich in bioactive compounds in the form of vitamins, phycobilins, polyphenols, carotenoids, phycocyanins and polysaccharides; many of these are known to have advantageous applications in human health. 3-Hydroxy-4,7-megastigmadien-9-one (comp) was isolated from Ulva pertusa (U. pertusa) Kjellman (Ulvaceae), which is a familiar edible green seaweed. Objective: This study evalua ...
DOI:
10.1080/13880209.2016.1246574
PubMed:
27937044
PubMed Central:
PMC6130523

4. 3D origami electrochemical device for sensitive Pb2+ testing based on DNA functionalized iron-porphyrinic metal-organic framework

Author:
Xiu Wang; Chunlei Yang; Shaojun Zhu; Mei Yan; Shenguang Ge; Jinghua Yu
Source:
Biosensors & bioelectronics 2017 v.87 pp. 108-115
ISSN:
0956-5663
Subject:
DNA; biosensors; detection limit; electrochemistry; electrodes; gold; lead; metal ions; nanogold; oligonucleotides; paper; peroxidase
Abstract:
... A highly sensitive electrochemical (EC) biosensor combined with a 3D origami device for detection of Pb²⁺was developed based on novel Au nanoparticles modified paper working electrode (Au-PWE) as sensor platform and DNA functionalized iron-porphyrinic metal-organic framework ((Fe-P)n-MOF-Au-GR) hybrids as signal probes. In the presence of Pb²⁺, GR could be specifically cleaved at the ribonucleotid ...
DOI:
10.1016/j.bios.2016.08.016

5. 4-Nonyphenol induced apoptosis and DNA damage in the African catfish Clarias gariepinus, the antioxidant role of Cydonia oblonga

Author:
Alaa El-Din H. Sayed; Heba S. Hamed
Source:
Ecotoxicology and environmental safety 2017 pp. -
ISSN:
0147-6513
Subject:
aspartate transaminase; DNA damage; lipid peroxidation; creatinine; quinces; blood chemistry; biomarkers; alanine transaminase; glucose; poisoning; apoptosis; albumins; NADP-glucose-6-phosphate dehydrogenase; superoxide dismutase; Clarias gariepinus; toxicity; enzyme activity; antioxidants; leaf extracts; liver; DNA fragmentation; Cydonia oblonga; catalase; uric acid; erythrocytes; cholesterol; urea; catfish; protein content; globulins; alkaline phosphatase
Abstract:
... The present study was planned to assess the toxic effects of sublethal concentration (0.1 mgl−1) 4-nonylphenol (4-NP) on serum biochemical parameters, liver lipid peroxidation (LPO) and the antioxidant enzymes of the African catfish Clarias gariepinus for 14 days and the ability of the quince leaf extract to alleviate the effects of (4-NP) on C. gariepinus. Fish were categorized into four groups: ...
DOI:
10.1016/j.ecoenv.2017.05.013

6. 5-Aminolevulinic acid improves DNA damage and DNA Methylation changes in deltamethrin-exposed Phaseolus vulgaris seedlings

Author:
Mahmut Sinan Taspinar; Murat Aydin; Esra Arslan; Muhammet Yaprak; Guleray Agar
Source:
Plant physiology and biochemistry 2017 v.118 pp. 267-273
ISSN:
0981-9428
Subject:
DNA; DNA damage; DNA methylation; Phaseolus vulgaris; adverse effects; aminolevulinic acid; deltamethrin; mutagens; plant growth substances; pyrethrins; random amplified polymorphic DNA technique; restriction endonucleases; seedlings
Abstract:
... Deltamethrin, synthetic type II pyrethroid, is one of the most widely used pesticide in agriculture. Intense use of deltamethrin can cause permanant or temporary damages in nontarget plant species. In this study, we aimed to determine DNA methylation change and DNA damage level in Phaseolus vulgaris seedlings subjected to different concentrations of deltamethrin (0.02, 0.1 and 0.5 ppm). Coupled Re ...
DOI:
10.1016/j.plaphy.2017.06.026

7. 5-Selenocyanatouracil: A Potential Hypoxic Radiosensitizer. Electron Attachment Induced Formation of Selenium Centered Radical

Author:
Sosnowska Marta; Makurat Samanta; Zdrowowicz Magdalena; Rak Janusz
Source:
Journal of physical chemistry 2017 v.121 no.25 pp. 6139-6147
ISSN:
1520-5207
Subject:
DNA; Gibbs free energy; X-radiation; acetic acid; activation energy; chlorides; crosslinking; cytotoxicity; energy; high performance liquid chromatography; hydroxyl radicals; selenium; uracil
Abstract:
... The propensity of 5-selenocyanatouracil (SeCNU) to decomposition induced by attachment of electron was scrutinized with the G3B3 composite quantum-chemical method and radiolytic studies. Favorable thermodynamic (Gibbs free reaction energy of −13.65 kcal/mol) and kinetic (Gibbs free activation energy of 1.22 kcal/mol) characteristics revealed by the G3B3 free energy profile suggest SeCNU to be sens ...
DOI:
10.1021/acs.jpcb.7b03633

8. 7-Glutathione-pyrrole and 7-cysteine-pyrrole are potential carcinogenic metabolites of pyrrolizidine alkaloids

Author:
Xiaobo He; Qingsu Xia; Peter P. Fu
Source:
Journal of environmental science and health 2017 v.35 no.2 pp. 69-83
ISSN:
1532-4095
Subject:
DNA; hepatotoxicity; human cell lines; humans; liver neoplasms; metabolism; metabolites; mutagens; phytopharmaceuticals; pyrrolizidine alkaloids; rats
Abstract:
... Many pyrrolizidine alkaloids (PAs) are hepatotoxic, genotoxic, and carcinogenic phytochemicals. Metabolism of PAs in vivo generates four (±)-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5 H -pyrrolizine (DHP)-DNA adducts that have been proposed to be responsible for PA-induced liver tumor formation in rats. In this present study, we determined that the same set of DHP-DNA adducts was formed upon the incu ...
DOI:
10.1080/10590501.2017.1298358

9. Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times

Author:
Anna Lena Flux; Janine Mazanec; Birgit Strommenger; Susanne Hummel
Source:
Diversity 2017 v.9 no.4 pp. -
ISSN:
1424-2818
Subject:
DNA; antibiotics; evolution; genes; methicillin-resistant Staphylococcus aureus; microorganisms; necropsy; nucleotide sequences; osteomyelitis; pathogens; polymerase chain reaction; public health
Abstract:
... Staphylococcus aureus is a major pathogen causing osteomyelitis, amongst other diseases, and its methicillin-resistant form (MRSA) in particular poses a huge threat to public health. To increase our knowledge of the origin and evolution of S. aureus, genetic studies of historical microorganisms may be beneficial. Thus, the aim of this study was to investigate whether osteomyelitic skeletal materia ...
DOI:
10.3390/d9040043

10. A Broad-Spectrum Inhibitor of CRISPR-Cas9

Author:
Lucas B. Harrington; Kevin W. Doxzen; Enbo Ma; Jun-Jie Liu; Gavin J. Knott; Alireza Edraki; Bianca Garcia; Nadia Amrani; Janice S. Chen; Joshua C. Cofsky; Philip J. Kranzusch; Erik J. Sontheimer; Alan R. Davidson; Karen L. Maxwell; Jennifer A. Doudna
Source:
Cell 2017 v.170 pp. 1224-1233.e15
ISSN:
0092-8674
Subject:
DNA; active sites; crystal structure; dimerization; gene editing; immune system; proteins
Abstract:
... CRISPR-Cas9 proteins function within bacterial immune systems to target and destroy invasive DNA and have been harnessed as a robust technology for genome editing. Small bacteriophage-encoded anti-CRISPR proteins (Acrs) can inactivate Cas9, providing an efficient off switch for Cas9-based applications. Here, we show that two Acrs, AcrIIC1 and AcrIIC3, inhibit Cas9 by distinct strategies. AcrIIC1 i ...
DOI:
10.1016/j.cell.2017.07.037
PubMed:
28844692
PubMed Central:
PMC5875921

11. A DNA sequence obtained by replacement of the dopamine RNA aptamer bases is not an aptamer

Author:
Isabel Álvarez-Martos; Elena E. Ferapontova
Source:
Biochemical and biophysical research communications 2017 v.489 pp. 381-385
ISSN:
0006-291X
Subject:
DNA; RNA; biosensors; chemical elements; dopamine; neurotransmitters; nucleotide sequences; oligonucleotides
Abstract:
... A unique specificity of the aptamer-ligand biorecognition and binding facilitates bioanalysis and biosensor development, contributing to discrimination of structurally related molecules, such as dopamine and other catecholamine neurotransmitters. The aptamer sequence capable of specific binding of dopamine is a 57 nucleotides long RNA sequence reported in 1997 (Biochemistry, 1997, 36, 9726). Later ...
DOI:
10.1016/j.bbrc.2017.05.134

12. A DNA test for routine prediction in breeding of peach blush, Ppe-Rf-SSR

Author:
Paul Sandefur; Terrence Frett; John Clark; Ksenija Gasic; Cameron Peace
Source:
Molecular breeding 2017 v.37 no.1 pp. 11
ISSN:
1380-3743
Subject:
DNA; Prunus persica; breeding; breeding programs; color; fresh market; genetic markers; germplasm; haplotypes; loci; microsatellite repeats; parents; peaches; polymerase chain reaction; prediction; reproductive performance; screening; seedlings; single nucleotide polymorphism
Abstract:
... Blush, the proportion of red overcolor on the skin surface of fruit, is highly variable in peach breeding germplasm and is important in the marketing of peach fruit. The fresh market peach industry demands a high level of blush to entice consumers, while the processing peach industry requires minimal blush. Therefore, blush is a major selection criterion in breeding programs. The use of DNA-based ...
DOI:
10.1007/s11032-016-0615-3
CHORUS:
10.1007/s11032-016-0615-3

13. A DNA-stabilized silver nanoclusters/graphene oxide-based platform for the sensitive detection of DNA through hybridization chain reaction

Author:
Siqi Zhang; Kun Wang; Kai-Bin Li; Wei Shi; Wen-Ping Jia; Xiaoying Chen; Ting Sun; De-Man Han
Source:
Biosensors & bioelectronics 2017 v.91 pp. 374-379
ISSN:
0956-5663
Subject:
DNA; DNA probes; Human immunodeficiency virus; adsorption; biosensors; detection limit; fluorescence; nanoparticles; nanowires; nucleotide sequences; silver nitrate
Abstract:
... A silver nanoclusters (AgNCs)/graphene oxide (GO)-based fluorescence sensor was developed for label-free DNA detection through hybridization chain reaction (HCR). A DNA sequence associated with the human immunodeficiency virus (HIV) was selected as a model target. Two DNA probes, hairpin probe 1 (H1) and hairpin probe 2 (H2), were partially complementary. GO was used as an adsorption material to c ...
DOI:
10.1016/j.bios.2016.12.060

14. A Determined “Hesitation” on H3K27me3 Empowers Stem Cells to Differentiate

Author:
Xin Huang; Jianlong Wang
Source:
Molecular cell 2017 v.66 no.2 pp. 165-166
ISSN:
1097-2765
Subject:
cell differentiation; DNA; stem cells; transcription factors
Abstract:
... To uncover the precise mechanisms coordinating proliferation and fate choice of stem cells, in this issue of Molecular Cell and in an accompanying paper in Cell Reports, Mazo and colleagues (Petruk et al. 2017a, 2017b) reveal that delayed accumulation of H3K27me3 on nascent DNA is essential to recruit pioneer transcription factors in stem cell differentiation. ...
DOI:
10.1016/j.molcel.2017.04.003

15. A Dimorphic and Virulence-Enhancing Endosymbiont Bacterium Discovered in Rhizoctonia solani

Author:
Ken Obasa; Frank F. White; John Fellers; Megan Kennelly; Sanzhen Liu; Benjamin Katz; John Tomich; David Moore; Heather Shinogle; Karen Kelley
Source:
Phytobiomes journal 2017 v.1 no.1 pp. 14-23
ISSN:
2471-2906
Subject:
Agrostis stolonifera; DNA; Enterobacter; Rhizoctonia; Thanatephorus cucumeris; bacteria; cold season; electron microscopy; endosymbionts; fungi; host plants; microsymbionts; phenylacetic acid; phytobiome; turf grasses; virulence
Abstract:
... The ubiquitous soilborne and plant-pathogenic basidiomycete Rhizoctonia solani, although classified as a single species, is a complex of anastomosis groups (AGs) that cause disease in a broad range of higher plants. Here, we investigated the persistent co-isolation of bacteria with R. solani from brown patch-infected, cool-season turfgrasses, and report the presence of endo-hyphal bacteria, relate ...
DOI:
10.1094/PBIOMES-08-16-0005-R

16. A Microdevice for Rapid, Monoplex and Colorimetric Detection of Foodborne Pathogens using a Centrifugal Microfluidic Platform

Author:
Abkar Sayad; Fatimah Ibrahim; Shah Mukim Uddin; Jongman Cho; Marc Madou; Kwai Lin Thong
Source:
Biosensors & bioelectronics 2017 pp. -
ISSN:
0956-5663
Subject:
DNA; Escherichia; Salmonella; Vibrio cholerae; affordability; bacteria; bacterial contamination; biosensors; chicken meat; color; colorimetry; detection limit; food contamination; food pathogens; foodborne illness; genetic analysis; microbial detection; mixing; point-of-care systems; risk reduction; wireless technology
Abstract:
... Outbreaks of foodborne diseases have become a global health concern; hence, many improvements and developments have been made to reduce the risk of food contamination. We developed a centrifugal microfluidic automatic wireless endpoint detection system integrated with loop mediated isothermal amplification (LAMP) for monoplex pathogen detection. Six identical sets were designed on the microfluidic ...
DOI:
10.1016/j.bios.2017.08.060

17. A Molecular Prospective for HIRA Complex Assembly and H3.3-Specific Histone Chaperone Function

Author:
M. Daniel Ricketts; Ronen Marmorstein
Source:
Journal of Molecular Biology 2017 v.429 pp. 1924-1933
ISSN:
0022-2836
Subject:
DNA; cell cycle; genes; heterochromatin; histones; humans; models; post-translational modification; transcription (genetics)
Abstract:
... Incorporation of variant histone sequences, in addition to post-translational modification of histones, serves to modulate the chromatin environment. Different histone chaperone proteins mediate the storage and chromatin deposition of variant histones. Although the two non-centromeric histone H3 variants, H3.1 and H3.3, differ by only 5 aa, replacement of histone H3.1 with H3.3 can modulate the tr ...
DOI:
10.1016/j.jmb.2016.11.010
PubMed:
27871933
PubMed Central:
PMC5438300

18. A More Comprehensive Community of Ammonia-Oxidizing Archaea (AOA) Revealed by Genomic DNA and RNA Analyses of amoA Gene in Subtropical Acidic Forest Soils

Author:
Ruo-Nan Wu; Han Meng; Yong-Feng Wang; Wensheng Lan; Ji-Dong Gu
Source:
Microbial ecology 2017 v.74 no.4 pp. 910-922
ISSN:
0095-3628
Subject:
Archaea; DNA; RNA; acid soils; aluminum; ammonia; coprecipitation; dissolved organic matter; environmental factors; forest soils; genes; nitrification; nitrifying bacteria; nitrogen; oxidants; pH; phylogeny; phylotype; toxicity; trees
Abstract:
... Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are the main nitrifiers which are well studied in natural environments, and AOA frequently outnumber AOB by orders especially in acidic conditions, making AOA the most promising ammonia oxidizers. The phylogeny of AOA revealed in related studies, however, often varied and hardly reach a consensus on functional phylotypes. The objective of this stu ...
DOI:
10.1007/s00248-017-1045-4

19. A New Perspective on Polyploid Fragaria (Strawberry) Genome Composition Based on Large-Scale, Multi-Locus Phylogenetic Analysis

Author:
Yilong Yang; Thomas M. Davis
Source:
Genome Biology and Evolution 2017 v.9 no.12 pp. 3433-3448
ISSN:
1759-6653
Subject:
DNA; alleles; allopolyploidy; diploidy; genomics; octaploidy; phylogeny; strawberries
Abstract:
... The subgenomic compositions of the octoploid (2n = 8× = 56) strawberry (Fragaria) species, including the economically important cultivated species Fragaria x ananassa, have been a topic of long-standing interest. Phylogenomic approaches utilizing next-generation sequencing technologies offer a new window into species relationships and the subgenomic compositions of polyploids. We have conducted a ...
DOI:
10.1093/gbe/evx214
CHORUS:
10.1093/gbe/evx214

20. A Peptide Cleavage-Based Ultrasensitive Electrochemical Biosensor with an Ingenious Two-Stage DNA Template for Highly Efficient DNA Exponential Amplification

Author:
Wang Ding; Chai Yaqin; Yuan Yali; Yuan Ruo
Source:
Analytical chemistry 2017 v.89 no.17 pp. 8951-8956
ISSN:
1520-6882
Subject:
DNA; amino acids; biosensors; detection limit; models
Abstract:
... The direct transduction of a peptide cleavage event into DNA detection has always produced output DNA with some amino acid residues, which influence the DNA amplification efficiency in view of their steric hindrance effect. Here an ingenious two-stage DNA template was designed to achieve highly efficient DNA amplification by utilizing the DNA exponential amplification reaction (EXPAR) as a model. ...
DOI:
10.1021/acs.analchem.7b01477