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You searched for: Subject "antigen-antibody complex" Remove constraint Subject: "antigen-antibody complex" Publication Year 2017 Remove constraint Publication Year: 2017 Subject antibodies Remove constraint Subject: antibodies
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1. High-sensitivity immunochromatographic assay for fumonisin B1 based on indirect antibody labeling

Author:
Urusov, Alexandr E.; Petrakova, Alina V.; Gubaydullina, Milyausha K.; Zherdev, Anatoly V.; Eremin, Sergei A.; Kong, Dezhao; Liu, Liqiang; Xu, Chuanlai; Dzantiev, Boris B.
Source:
Biotechnology letters 2017 v.39 no.5 pp. 751-758
ISSN:
0141-5492
Subject:
antigen-antibody complex,   etc   ; antibodies; corn; fumonisin B1; geometry; immunoaffinity chromatography; nanogold; plant extracts;     Show all 8 Subjects
Abstract:
... OBJECTIVE: To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts. RESULTS: Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for th ...
DOI:
10.1007/s10529-017-2294-5

2. The Use of Fluoroproline in MUC1 Antigen Enables Efficient Detection of Antibodies in Patients with Prostate Cancer

Author:
Somovilla, Víctor J.; Bermejo, Iris A.; Albuquerque, Inês S.; Martínez-Sáez, Nuria; Castro-López, Jorge; García-Martín, Fayna; Compañón, Ismael; Hinou, Hiroshi; Nishimura, Shin-Ichiro; Jiménez-Barbero, Jesús; Asensio, Juan L.; Avenoza, Alberto; Busto, Jesús H.; Hurtado-Guerrero, Ramón; Peregrina, Jesús M.; Bernardes, Gonçalo J. L.; Corzana, Francisco
Source:
Journal of the American Chemical Society 2017 v.139 no.50 pp. 18255-18261
ISSN:
1520-5126
Subject:
antigen-antibody complex,   etc   ; X-ray diffraction; antibodies; antigens; blood serum; diagnostic techniques; glycopeptides; interferometry; patients; proline; prostatic neoplasms;     Show all 11 Subjects
Abstract:
... A structure-based design of a new generation of tumor-associated glycopeptides with improved affinity against two anti-MUC1 antibodies is described. These unique antigens feature a fluorinated proline residue, such as a (4S)-4-fluoro-l-proline or 4,4-difluoro-l-proline, at the most immunogenic domain. Binding assays using biolayer interferometry reveal 3-fold to 10-fold affinity improvement with r ...
DOI:
10.1021/jacs.7b09447

3. Biochemical-immunological hybrid biosensor based on two-dimensional chromatography for on-site sepsis diagnosis

Author:
Kim, Seung-Wan; Cho, Il-Hoon; Lim, Guei-Sam; Park, Gi-Na; Paek, Se-Hwan
Source:
Biosensors & bioelectronics 2017 v.98 pp. 7-14
ISSN:
0956-5663
Subject:
antigen-antibody complex,   etc   ; C-reactive protein; antibodies; biosensors; blood serum; chromatography; color; immunoassays; lactic acid; tracer techniques;     Show all 10 Subjects
Abstract:
... A hybrid-biosensor system that can simultaneously fulfill the immunoassay for protein markers (e.g., C-reactive protein (CRP) and procalcitonin (PCT)) and the enzyme assay for metabolic substances (e.g., lactate) in the same sepsis-based sample has been devised. Such a challenge was pursued through the installation of an enzyme-reaction zone on the signal pad of the typical immuno-strip for the ra ...
DOI:
10.1016/j.bios.2017.06.032

4. Application of magnetite nanoparticles for the development of highly sensitive immunochromatographic test systems for mycotoxin detection

Author:
Petrakova, A. V.; Urusov, A. E.; Zherdev, A. V.; Liu, L.; Xu, C.; Dzantiev, B. B.
Source:
Applied biochemistry and microbiology 2017 v.53 no.4 pp. 470-475
ISSN:
0003-6838
Subject:
antigen-antibody complex,   etc   ; T-2 toxin; antibodies; detection limit; immunoaffinity chromatography; infant foods; magnetite; nanoparticles; oats; zearalenone;     Show all 10 Subjects
Abstract:
... Highly sensitive immunochromatographic test systems were developed for the detection of zearalenone (ZEA) and T-2 toxin (T2T) using magnetite nanoparticles (MNPs) for the labeling. In order to detect an analyte with high sensitivity, the competitive reaction was performed with free specific antibodies, while immune complexes were detected by the reaction with label-conjugated anti-species antibodi ...
DOI:
10.1134/S0003683817040111

5. Mathematical Model of Serodiagnostic Immunochromatographic Assay

Author:
Sotnikov, Dmitriy V.; Zherdev, Anatoly V.; Dzantiev, Boris B.
Source:
Analytical chemistry 2017 v.89 no.8 pp. 4419-4427
ISSN:
1520-6882
Subject:
antigen-antibody complex,   etc   ; antibodies; antigens; blood serum; computer software; detection limit; immunoaffinity chromatography; immunoglobulins; mathematical models; serodiagnosis;     Show all 10 Subjects
Abstract:
... This article describes the mathematical model for an immunochromatographic assay for the detection of specific immunoglobulins against a target antigen (antibodies) in blood/serum (serodiagnosis). The model utilizes an analytical (non-numerical) approach and allows the calculation of the kinetics of immune complexes’ formation in a continuous-flow system using commonly available software, such as ...
DOI:
10.1021/acs.analchem.6b03635

6. Kinetic turbidimetry of patchy colloids aggregation: Latex particles immunoagglutination A Physicochemical and engineering aspects

Author:
Polshchitsin, Alexey A.; Nekrasov, Vyacheslav M.; Zakovryashin, Valentin S.; Yakovleva, Galina E.; Maltsev, Valeri P.; Yurkin, Maxim A.; Chernyshev, Andrei V.
Source:
Colloids and surfaces 2017 v.516 pp. 72-79
ISSN:
0927-7757
Subject:
antigen-antibody complex,   etc   ; C-reactive protein; absorbance; active sites; algorithms; antibodies; colloids; equations; immunoglobulin G; latex; light scattering; polystyrenes;     Show all 12 Subjects
Abstract:
... The paper is devoted to the problem of the kinetic study of aggregation of particles with several active sites (so called “patchy particles”) using turbidimetry. Patchy particles are known as chemically-anisotropic reactants, and the kinetics theory of their interaction is far from complete. In this work we theoretically derived and experimentally verified analytical expressions, which are conveni ...
DOI:
10.1016/j.colsurfa.2016.12.018

7. Prevalence, severity, and pathogeneses of anemia in visceral leishmaniasis

Author:
Goto, Yasuyuki; Cheng, Jingjie; Omachi, Satoko; Morimoto, Ayako
Source:
Parasitology research 2017 v.116 no.2 pp. 457-464
ISSN:
0932-0113
Subject:
antigen-antibody complex,   etc   ; anemia; antibodies; antileishmanials; bone marrow; erythrocytes; erythropoiesis; hamsters; hemoglobin; hemolysis; mice; pathogenesis; patients; spleen; visceral leishmaniasis;     Show all 15 Subjects
Abstract:
... Anemia is a typical symptom during visceral leishmaniasis (VL). We performed a systematic analysis of the literature on anemia in VL to understand the prevalence, severity, and possible mechanisms. Anemia is very common in VL patients with an overall prevalence higher than 90 %. The degree of anemia in VL is moderate to severe (hemoglobin level ∼7.5 g/dl), and the status can be recovered by treatm ...
DOI:
10.1007/s00436-016-5313-x

8. New approach for monitoring fish stress: A novel enzyme-functionalized label-free immunosensor system for detecting cortisol levels in fish

Author:
Wu, Haiyun; Ohnuki, Hitoshi; Ota, Shirei; Murata, Masataka; Yoshiura, Yasutoshi; Endo, Hideaki
Source:
Biosensors & bioelectronics 2017 v.93 pp. 57-64
ISSN:
0956-5663
Subject:
antigen-antibody complex,   etc   ; Oreochromis niloticus; antibodies; cortisol; electrochemistry; electrodes; enzyme-linked immunosorbent assay; fish; fish farms; glucose; glucose oxidase; gold; immunosensors; monitoring; oxidation; rapid methods; stress response;     Show all 17 Subjects
Abstract:
... Fishes display a wide variation in their physiological responses to stress, which is clearly evident in the plasma corticosteroid changes, chiefly cortisol levels in fish. As a well-known indicator of fish stress, a simple and rapid method for detecting cortisol changes especially sudden increases is desired. In this study, we describe an enzyme-functionalized label-free immunosensor system for de ...
DOI:
10.1016/j.bios.2016.10.001

9. A highly sensitive capillary electrophoresis immunoassay strategy based on dual‐labeled gold nanoparticles enhancing chemiluminescence for the detection of prostate‐specific antigen

Author:
Li, Shuting; Shi, Min; Zhao, Jingjin; Zhang, Liangliang; Huang, Yong; Zhao, Shulin
Source:
Electrophoresis 2017 v.38 no.13-14 pp. 1780-1787
ISSN:
0173-0835
Subject:
antigen-antibody complex,   etc   ; antibodies; antigens; blood serum; capillary electrophoresis; chemiluminescence; detection limit; humans; immune response; immunoassays; nanogold; patients; peroxidase; prostate-specific antigen; prostatic neoplasms; silver;     Show all 16 Subjects
Abstract:
... An enzyme and antibody dual labeled gold nanoparticles enhancing chemiluminescence strategy was developed for highly sensitive CE immunoassay (IA) of prostate‐specific antigen (PSA). In this work, gold nanoparticles were labeled with horseradish peroxidase and antiprostate specific antigen‐antibody, and used as the marker (Ab*). After PSA (antigen, Ag) was added into the system, a noncompetitive i ...
DOI:
10.1002/elps.201600396

10. Chemiluminescence noncompetitive immunoassay based on microchip electrophoresis for the determination of β-subunit of human chorionic gonadotropin B Analytical technologies in the biomedical and life sciences

Author:
Li, Shuting; Yang, Tingzheng; Zhao, Jingjin; Huang, Yong; Zhao, Shulin
Source:
Journal of chromatography 2017 v.1053 pp. 42-47
ISSN:
1570-0232
Subject:
antigen-antibody complex,   etc   ; animal ovaries; antibodies; antigens; blood serum; chemiluminescence; chromatography; detection limit; electrophoresis; human chorionic gonadotropin; humans; immunoassays; ovarian neoplasms; patients; peroxidase;     Show all 15 Subjects
Abstract:
... In this work, a microchip-electrophoresis chemiluminescence (MCE-CL)-based immunoassay method was established for the determination of β-subunit of human chorionic gonadotropin (β-HCG). This approach uses MCE-CL assay as a platform. First, the β-HCG antigen (Ag) binds to excess horseradish peroxidase (HRP)-labeled anti-β-HCG antibodies (Ab*) to form an immune complex (Ag-Ab*). Subsequently, the ob ...
DOI:
10.1016/j.jchromb.2017.03.031

11. A systematic study of the effect of low pH acid treatment on anti-drug antibodies specific for a domain antibody therapeutic: Impact on drug tolerance, assay sensitivity and post-validation method assessment of ADA in clinical serum samples

Author:
Kavita, Uma; Duo, Jia; Crawford, Sean M.; Liu, Rong; Valcin, Joan; Gleason, Carol; Dong, Huijin; Gadkari, Snaehal; Dodge, Robert W.; Pillutla, Renuka C.; DeSilva, Binodh S.
Source:
Journal of Immunological Methods 2017
ISSN:
0022-1759
Subject:
antigen-antibody complex,   etc   ; acid treatment; acids; antibodies; blood serum; chemiluminescence; clinical trials; data collection; dissociation; dose response; drug resistance; drugs; humans; immunoassays; pH;     Show all 15 Subjects
Abstract:
... We developed a homogeneous bridging anti-drug antibody (ADA) assay on an electro chemiluminescent immunoassay (ECLIA) platform to support the immunogenicity evaluation of a dimeric domain antibody (dAb) therapeutic in clinical studies. During method development we evaluated the impact of different types of acid at various pH levels on polyclonal and monoclonal ADA controls of differing affinities ...
DOI:
10.1016/j.jim.2017.06.002

12. SIgA–Shigella Immune Complexes Interact with Dectin-1 and SIGNR3 to Differentially Regulate Mouse Peyer's Patch and Mesenteric Lymph Node Dendritic Cell's Responsiveness

Author:
Mikulic, Josip; Bioley, Gilles; Corthésy, Blaise
Source:
Journal of Molecular Biology 2017
ISSN:
0022-2836
Subject:
antigen-antibody complex,   etc   ; Peyer's patches; Shigella flexneri; antibodies; chemokines; dendritic cells; enteropathogens; glycosylation; homeostasis; immune response; immunoglobulin A; intestines; lymph nodes; mice; mucosa; receptors;     Show all 16 Subjects
Abstract:
... In addition to contributing to immune exclusion at mucosal surfaces, secretory IgA (SIgA) made of polymeric IgA and secretory component is able to selectively reenter via microfold cells into Peyer's patches (PPs) present along the intestine and to associate with dendritic cells (DCs) of the CD11c+CD11b+MHCII+F4/80−CD8−phenotype in the subepithelial dome region and the draining mesenteric lymph no ...
DOI:
10.1016/j.jmb.2017.05.024

13. Impact of heat treatment on antigen detection in sera of Angiostrongylus vasorum infected dogs

Author:
Gillis-Germitsch, Nina; Schnyder, Manuela
Source:
Parasites & vectors 2017 v.10 no.1 pp. 421
ISSN:
1756-3305
Subject:
antigen-antibody complex,   etc   ; Angiostrongylus vasorum; EDTA (chelating agent); angiostrongylosis; antibodies; antibody detection; antigen detection; antigens; dog diseases; dogs; enzyme-linked immunosorbent assay; epidemiological studies; heat treatment; necropsy; parasites; seroprevalence;     Show all 16 Subjects
Abstract:
... BACKGROUND: In the last decade serological tests for detection of circulating Angiostrongylus vasorum antigen and specific antibodies have been developed and adopted for individual diagnosis and epidemiological studies in dogs. Although confirmed positive at necropsy, antigen detection was not possible in single experimentally, as well as naturally infected dogs, possibly due to immune complex for ...
DOI:
10.1186/s13071-017-2366-3
PubMed:
28915846
PubMed Central:
PMC5602946

14. Validation of immune complex dissociation methods for use with heartworm antigen tests

Author:
Beall, Melissa J.; Arguello-Marin, Andrea; Drexel, Jan; Liu, Jiayou; Chandrashekar, Ramaswamy; Alleman, A. Rick
Source:
Parasites & vectors 2017 v.10 no.Supplement 2 pp. 481
ISSN:
1756-3305
Subject:
antigen-antibody complex,   etc   ; Dirofilaria immitis; EDTA (chelating agent); acid treatment; antibodies; antigen detection; antigens; blood serum; diagnostic techniques; dissociation; dogs; doxycycline; enzyme-linked immunosorbent assay; heartworms; heat treatment; pH; patients; pepsin; pets; prospective studies; therapeutics;     Show all 21 Subjects
Abstract:
... BACKGROUND: Antigen testing is routinely used to diagnose canine Dirofilaria immitis infections. Immune complex dissociation (ICD) methods, which were employed in the original heartworm antigen tests to release antigen that was bound by endogenous canine antibodies, were discontinued with improvements in assay reagents. The purpose of this study was to evaluate different ICD methods for detection ...
DOI:
10.1186/s13071-017-2442-8
PubMed:
29143646
PubMed Central:
PMC5688509

15. Canine rheumatoid arthritis characterized by hyperprolactinemia

Author:
Kimura, Tohru
Source:
Veterinary science development 2017 v.7 no.1
ISSN:
2038-9698
Subject:
antigen-antibody complex,   etc   ; DNA; albumins; antibodies; blood coagulation; blood proteins; blood serum; complement; dogs; elbows; electrophoresis; females; milk; prolactin; protein content; rheumatoid arthritis; risk; signs and symptoms (animals and humans); stifle; suckling;     Show all 20 Subjects
Abstract:
... The senile female dog suffering from rheumatoid arthritis was clinically and clinicopathologically examined. The articular lesions localized in the elbows, stifles, and the carpal and tarsal joints. The dog with clinical signs of arthritis developed draining nodular lesions in the involved joints. The affected joints became swollen, and then serous exudates were observed in their ulcerative lesion ...
DOI:
10.4081/vsd.2017.6463