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1. Mutant Potential Ubiquitination Sites in Dur3p Enhance the Urea and Ethyl Carbamate Reduction in a Model Rice Wine System

Author:
Zou, Huijun,   et al.   ; Zhang, Peng; Du, Guocheng; Xie, Guangfa; Chen, Jian; Shi, Zhongping; Zhou, Jingwen;     Show all 7 Authors
Source:
Journal of agricultural and food chemistry 2017 v.65 no.8 pp. 1641-1648
ISSN:
1520-5118
Subject:
Saccharomyces cerevisiae; arginine; carcinogens; endocytosis; engineering; ethyl carbamate; glutamine; lysine; models; mutants; mutation; nitrogen; plasma membrane; rice wines; transporters; ubiquitination; urea; yeasts
Abstract:
... Ubiquitination can significantly affect the endocytosis and degradation of plasma membrane proteins. Here, the ubiquitination of a Saccharomyces cerevisiae urea plasma membrane transporter (Dur3p) was altered. Two potential ubiquitination sites, lysine residues K556 and K571, of Dur3p were predicted and replaced by arginine, and the effects of these mutations on urea utilization and formation unde ...
DOI:
10.1021/acs.jafc.6b05348