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You searched for: Publication year rev 7983-2017 Remove constraint Publication year rev: 7983-2017 Subject term cytoskeleton Remove constraint Subject term: cytoskeleton
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1. A Cyclin D2-derived peptide acts on specific cell cycle phases by activating ERK1/2 to cause the death of breast cancer cells

Author:
Lilian C. Russo; Christiane B. Araujo; Leo K. Iwai; Emer S. Ferro; Fabio L. Forti
Source:
Journal of proteomics 2017 v.151 pp. 24-32
ISSN:
1874-3919
Subject:
affinity chromatography; apoptosis; breast neoplasms; cell proliferation; confocal microscopy; cyclins; cytoskeleton; death; humans; interphase; mass spectrometry; medicinal properties; mitogen-activated protein kinase; neoplasm cells; peptides; phosphorylation; proteasome endopeptidase complex; protein degradation; rats
Abstract:
... Protein degradation by the proteasome generates functional intracellular peptides. Pep5, a peptide derived from Cyclin D2, induces cell death in tumor cell lines and reduces the volume of rat C6 glioblastoma tumors in vivo. Here, we chose the human MDA-MB-231 breast cancer cells to evaluate the mechanism of cell death induced by pep5 in different phases of the cell cycle. Fluorescently labeled pep ...
DOI:
10.1016/j.jprot.2016.06.028

2. A Noncanonical Binding Site in the EVH1 Domain of Vasodilator-Stimulated Phosphoprotein Regulates Its Interactions with the Proline Rich Region of Zyxin

Author:
Acevedo Lucila Andrea; Greenwood Alexander I.; Nicholson Linda K.
Source:
Biochemistry 2017 v.56 no.35 pp. 4626-4636
ISSN:
1520-4995
Subject:
actin; binding sites; cell movement; cytoskeleton; models; mutation; nuclear magnetic resonance spectroscopy; phosphoproteins; phosphorylation; proline; stoichiometry; titration
Abstract:
... Vasodilator-stimulated phosphoprotein (VASP) is a processive actin polymerase with roles in the control of cell shape and cell migration. Through interaction with the cytoskeletal adaptor protein Zyxin, VASP can localize to damaged stress fibers where it serves to repair and reinforce these structures. VASP localization is mediated by its N-terminal Ena/VASP homology (EVH1) domain, which binds to ...
DOI:
10.1021/acs.biochem.7b00618
PubMed:
28783324
PubMed Central:
PMC5724578

3. A Shotgun Proteomics Approach Reveals a New Toxic Role for Alzheimer’s Disease Aβ Peptide: Spliceosome Impairment

Author:
Nuzzo Domenico; Inguglia Luigi; Walters Jessica; Picone Pasquale; Di Carlo Marta
Source:
Journal of Proteome Research 2017 v.16 no.4 pp. 1526-1541
ISSN:
1535-3907
Subject:
Alzheimer disease; Western blotting; biogenesis; bioinformatics; cytoskeleton; models; neurodegenerative diseases; pathogenesis; proteins; proteome; proteomics; spliceosomes; toxicity
Abstract:
... Proteomic changes have been described in many neurodegenerative diseases, including Alzheimer’s disease (AD). However, the early events in the onset of the pathology are yet to be fully elucidated. A cell model system in which LAN5 neuroblastoma cells were incubated for a short time with a recombinant form of Aβ₄₂ was utilized. Proteins extracted from these cells were subjected to shotgun proteomi ...
DOI:
10.1021/acs.jproteome.6b00925

4. A high-throughput QCM chip configuration for the study of living cells and cell-drug interactions

Author:
Haibo Shen; Tiean Zhou; Jiajin Hu
Source:
Analytical and bioanalytical chemistry 2017 v.409 no.27 pp. 6463-6473
ISSN:
1618-2642
Subject:
acoustics; cytoskeleton; cytotoxicity; drug evaluation; glass; human umbilical vein endothelial cells; isoprenaline; monitoring; paclitaxel; phenotype; quartz; screening; silicone; verapamil; viscoelasticity; viscosity
Abstract:
... In this study, we present a novel design of interference-free, negligible installation-induced stress, suitable for the fabrication of high-throughput quartz crystal microbalance (HQCM) chips. This novel HQCM chip configuration was fabricated using eight independent yet same-batch quartz crystal resonators within a common glass substrate with eight through-holes of diameter slightly larger than th ...
DOI:
10.1007/s00216-017-0591-4

5. A novel ex vivo immunoproteomic approach characterising Fasciola hepatica tegumental antigens identified using immune antibody from resistant sheep

Author:
Timothy C. Cameron; Ira Cooke; Pierre Faou; Hayley Toet; David Piedrafita; Neil Young; Vignesh Rathinasamy; Travis Beddoe; Glenn Anderson; Robert Dempster; Terry W. Spithill
Source:
International journal for parasitology 2017 v.47 no.9 pp. 555-567
ISSN:
0020-7519
Subject:
Fasciola gigantica; Fasciola hepatica; adaptive immunity; adults; amino acids; antibodies; antigens; cytoskeleton; cytotoxicity; definitive hosts; exocytosis; fascioliasis; flukes; glucose transporters; immune response; immunoglobulin G; immunotherapy; juveniles; lysosomes; mass spectrometry; metacercariae; multiple drug resistance; protein secretion; sequence homology; sheep; vaccines; vacuoles
Abstract:
... A more thorough understanding of the immunological interactions between Fasciola spp. and their hosts is required if we are to develop new immunotherapies to control fasciolosis. Deeper knowledge of the antigens that are the target of the acquired immune responses of definitive hosts against both Fasciola hepatica and Fasciola gigantica will potentially identify candidate vaccine antigens. Indones ...
DOI:
10.1016/j.ijpara.2017.02.004

6. A novel microfluidic device to model the human proximal tubule and glomerulus

Author:
Courtney M. Sakolish; Gretchen J. Mahler
Source:
RSC advances 2017 v.7 no.8 pp. 4216-4225
ISSN:
2046-2069
Subject:
blood proteins; cell lines; cytoskeleton; filtration; glomerular filtration rate; humans; kidneys; models; protein synthesis; shear stress
Abstract:
... We have developed a re-usable multi-layer microfluidic device to model the human kidney that incorporates a porous growth substrate, physiological fluid flow, and the passive filtration of the glomerulus. The target cell line in this project is HK-2, immortalized human kidney proximal tubule cells. Cells were exposed to a shear stress of 0.8 dyne cm⁻² within the device and monitored for protein ex ...
DOI:
10.1039/c6ra25641d

7. ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

Author:
Ewa Szypulska; Kamil Jankowski; Stanisław Weidner
Source:
Acta physiologiae plantarum 2017 v.39 no.8 pp. 190
ISSN:
0137-5881
Subject:
Hordeum vulgare; abscisic acid; actin; barley; biogenesis; biosynthesis; cell walls; cellulose; cytoskeleton; energy; energy metabolism; fruits; glycolysis; hemicellulose; lignification; lignin; messenger RNA; oxidative stress; protein metabolism; proteome; proteomics; salt stress; seedlings; signal transduction; sodium chloride; stress tolerance; translation (genetics)
Abstract:
... A proteomic study was conducted on caryopses of barley cv. Stratus (Hordeum vulgare). The caryopses were germinated in darkness at 20 °C, according to one of the three experimental setups: (a) in distilled water for 24 h, followed by 100 mM NaCl for another 24 h (salinity stress, SS); (b) in 100 µM abscisic acid for 24 h, with rinsing in distilled water to remove residual ABA, followed by 100 mM N ...
DOI:
10.1007/s11738-017-2490-x

8. Adhesion of bone marrow mesenchymal stem cells on porous titanium surfaces with strontium-doped hydroxyapatite coating

Author:
Dan-Li Fu; Qiao-hong Jiang; Fu-ming He; Bai-ping Fu
Source:
Journal of Zhejiang University 2017 v.18 no.9 pp. 778-788
ISSN:
1673-1581
Subject:
CD29 antigen; actin; bone marrow; bromides; cell adhesion; cell proliferation; cell viability; coatings; cytoskeleton; electrochemistry; fluorescence; gene expression; hydroxyapatite; non-specific protein-tyrosine kinase; quantitative polymerase chain reaction; rats; reverse transcriptase polymerase chain reaction; scanning electron microscopy; staining; stem cells; titanium; transcription (genetics)
Abstract:
... OBJECTIVE: To determine the adhesion behavior of bone marrow mesenchymal stem cells (MSCs) on a titanium surface with a nanostructured strontium-doped hydroxyapatite (Sr-HA) coating. METHODS: Sr-HA coatings were applied on roughened titanium surfaces using an electrochemical deposition method. Primary cultured rat MSCs were seeded onto Sr-HA-, HA-coated titanium, and roughened titanium surfaces, a ...
DOI:
10.1631/jzus.B1600517
PubMed Central:
PMC5611549

9. Analysis of differentially accumulated proteins associated with graft union formation in pecan (Carya illinoensis)

Author:
Zhenghai Mo; Haiyang He; Wenchuan Su; Fangren Peng
Source:
Scientia horticulturae 2017 v.224 pp. 126-134
ISSN:
0304-4238
Subject:
Carya illinoinensis; allografting; amino acids; aminocyclopropanecarboxylate oxidase; ascorbate peroxidase; callus; cytoskeleton; energy metabolism; ethylene production; juveniles; mass spectrometry; pecans; peroxiredoxin; protein metabolism; proteome; reactive oxygen species; ribosomal proteins; tracheary elements; tubulin; two-dimensional gel electrophoresis
Abstract:
... Grafting is of particular importance for the mass cultivation of pecan (Carya illinoensis), which can greatly shorten the long juvenile phase of this species. To reveal the molecular mechanism underlying graft union formation, the dynamic proteome changes at the graft union were investigated 0, 3, 8, 15, and 30days after grafting in homograft of pecan by two-dimensional gel electrophoresis. Forty- ...
DOI:
10.1016/j.scienta.2017.06.005

10. Andrographolide disrupts meiotic maturation by blocking cytoskeletal reorganisation and decreases the fertilisation potential of mouse oocytes

Author:
Hong-xing Liang; Sheng-sheng Lu; Zheng Yan; Yan-ping Kuang; Xiang-xing Zhu; Zhi-guang Yan; Tong Du; Wei-ran Chai; Hui Long; Qi-feng Lyu
Source:
Reproduction, fertility, and development 2017 v.29 no.12 pp. 2336-2344
ISSN:
1031-3613
Subject:
Andrographis paniculata; actin; adverse effects; andrographolide; apoptosis; cytokinesis; cytoskeleton; dose response; female fertility; infectious diseases; intracytoplasmic sperm injection; leaves; meiosis; mice; oocytes; Asia
Abstract:
... Andrographolide (AG) is a diterpenoid lactone isolated from the stem and leaves of Andrographis paniculata Nees that is used for the effective treatment of infectious diseases in Asian countries. Previous studies have reported adverse effects of AG on female fertility in rodents; however, the underlying mechanisms are unknown. The aim of the present study was to investigate the effects of AG on th ...
DOI:
10.1071/RD16343

11. Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows

Author:
Gagaoua Mohammed; Couvreur Sébastien; Le Bec Guillain; Aminot Ghislain; Picard Brigitte
Source:
Journal of agricultural and food chemistry 2017 v.65 no.17 pp. 3569-3580
ISSN:
1520-5118
Subject:
Maine-Anjou; beef; biomarkers; color; cows; cytoskeleton; enzymes; equations; heat shock proteins; longissimus muscle; metabolism; models; myofibrillar proteins; pH; protected designation of origin
Abstract:
... This study investigated the relationships among a list of 23 protein biomarkers with CIE-L*a*b* meat color traits and ultimate pH on Longissimus thoracis (LT) and Rectus abdominis (RA) muscles of 48 protected designation of origin Maine-Anjou cows. The technological parameters were correlated with several biomarkers and were in some cases muscle-dependent. More biomarkers were related to pHu in LT ...
DOI:
10.1021/acs.jafc.7b00434

12. Automated three-dimensional reconstruction of the Caenorhabditis elegans germline

Author:
Sandeep Gopal; Peter Boag; Roger Pocock
Source:
Developmental biology 2017 pp. -
ISSN:
0012-1606
Subject:
Caenorhabditis elegans; apoptosis; chromosomes; cytoskeleton; germ cells; methodology; models; mutation; phenotype; protein synthesis; proteins; stem cells
Abstract:
... The Caenorhabditis elegans germline is widely used as a model to study stem cell development, chromosome dynamics and apoptosis. Major readouts of germline phenotypes such as cell counting and protein expression profiling are routinely analysed manually and in a two-dimensional manner. The major disadvantages of the existing approaches are 1) they are time-consuming and laborious and 2) there is a ...
DOI:
10.1016/j.ydbio.2017.10.004

13. Bioinformatic search for Ca2+- and calmodulin-dependent protein kinases potentially associated with the regulation of plant cytoskeleton

Author:
D. O. Novozhylov; P. A. Karpov; Ya. B. Blume
Source:
Cytology and genetics 2017 v.51 no.4 pp. 239-246
ISSN:
0095-4527
Subject:
Arabidopsis thaliana; Homo sapiens; Mus musculus; active sites; animal proteins; animals; bioinformatics; calcium-calmodulin-dependent protein kinase; cytoskeleton; phylogeny
Abstract:
... The paper presents the results of a bioinformatic search for Ca²⁺- and calmodulin-dependent protein kinases from Arabidopsis thaliana, which may potentially participate in cytoskeleton regulation. Homologues were chosen based on their similarity with the calmodulin-dependent protein kinases from Homo sapiens and Mus musculus, which modulate the structure and dynamic behavior of the cytoskeleton. I ...
DOI:
10.3103/S0095452717040053

14. Blood-brain barrier dysfunction induced by silica NPs in vitro and in vivo: Involvement of oxidative stress and Rho-kinase/JNK signaling pathways

Author:
Xin Liu; Baiyan Sui; Jiao Sun
Source:
Biomaterials 2017 v.121 pp. 64-82
ISSN:
0142-9612
Subject:
adverse effects; astrocytes; blood-brain barrier; brain; circulatory system; cytoskeleton; endothelial cells; humans; in vivo studies; inflammation; models; nanoparticles; neurotoxicity; oxidative stress; rats; reactive oxygen species; signal transduction; silica; tight junctions; vascular endothelial growth factors
Abstract:
... Silica nanoparticles (SiO2-NPs) has been extensively exploited in biomedical fields and mostly designed to enter the circulatory system, however, few studies focused on the potential adverse effects of SiO2-NPs exposure on the blood-brain barrier (BBB) that serves as a critical barrier between the central nervous system (CNS) and the peripheral circulation. This study attempts to provide an unders ...
DOI:
10.1016/j.biomaterials.2017.01.006

15. Candida albicans Modifies the Protein Composition and Size Distribution of THP-1 Macrophage-Derived Extracellular Vesicles

Author:
Reales-Calderón Jose Antonio; Vaz Catarina; Monteoliva Lucía; Molero Gloria; Gil Concha
Source:
Journal of Proteome Research 2017 v.16 no.1 pp. 87-105
ISSN:
1535-3907
Subject:
Candida albicans; candidiasis; cytoskeleton; immune response; lipids; macrophages; messenger RNA; mitogen-activated protein kinase; monocytes; non-coding RNA; pathogens; protein composition; protein secretion; proteins; proteome; proteomics; tumor necrosis factor-alpha
Abstract:
... The effectiveness of macrophages in the response to systemic candidiasis is crucial to an effective clearance of the pathogen. The secretion of proteins, mRNAs, noncoding RNAs and lipids through extracellular vesicles (EVs) is one of the mechanisms of communication between immune cells. EVs change their cargo to mediate different responses, and may play a role in the response against infections. T ...
DOI:
10.1021/acs.jproteome.6b00605

16. Canonical and cellular pathways timing gamete release in Acropora digitifera, Okinawa, Japan

Author:
Y. Rosenberg; T. Doniger; S. Harii; F. Sinniger; O. Levy
Source:
Molecular ecology 2017 v.26 no.10 pp. 2698-2710
ISSN:
0962-1083
Subject:
Acropora; brooders; calcium signaling; cell movement; corals; cytoskeleton; focal adhesions; gene expression regulation; plants (botany); receptors; reproduction; reproductive behavior; solar radiation; spawning; transcriptome; Ryukyu Archipelago
Abstract:
... Natural light cycles are important for synchronizing behavioural and physiological rhythms over varying time periods in both plants and animals. An endogenous clock, regulated by positive and negative elements, interacting in feedback loops controls these rhythms. Many corals exhibit diel cycles of polyp expansion and contraction entrained by solar light patterns and monthly cycles of spawning or ...
DOI:
10.1111/mec.14062

17. Carbon black aggregates cause endothelial dysfunction by activating ROCK

Author:
Junyan Yan; Chia-Hsiang Lai; Shih-Chun Candice Lung; Wen-Cheng Wang; Chih-Ching Huang; Guan-Wen Chen; Guangli Suo; Cheng-Tai Choug; Chia-Hua Lin
Source:
Journal of hazardous materials 2017 v.338 pp. 66-75
ISSN:
0304-3894
Subject:
adverse effects; atherosclerosis; blood flow; cell viability; cytoskeleton; human cell lines; lactate dehydrogenase; nanoparticles; oxidative stress; soot; toxicity; transmission electron microscopy
Abstract:
... Carbon black nanoparticles (CBNs) have been associated with the progression of atherosclerosis. CBNs normally enter the bloodstream and crosslink together to form agglomerates. However, most studies have used nano-sized CB particles to clarify the involvement of CBN exposure in CBN-induced endothelial dysfunction. Herein, we studied endothelial toxicity of CBN aggregates (CBA) to human EA.hy926 va ...
DOI:
10.1016/j.jhazmat.2017.05.025

18. Cellular and molecular characterizations of a slow-growth variant provide insights into the fast growth of bamboo

Author:
Qiang Wei; Chen Jiao; Yulong Ding; Shan Gao; Lin Guo; Ming Chen; Pei Hu; Sujuan Xia; Guodong Ren; Zhangjun Fei
Source:
Tree physiology 2017 v.38 no.4 pp. 641-654
ISSN:
1758-4469
Subject:
Pseudosasa; auxins; bamboos; cell division; cell growth; cell walls; cellulose; cytoskeleton; developmental stages; extrusion; gene expression regulation; genes; genomics; internodes; mathematics; mutants; physiological transport; transcriptomics; tree physiology
Abstract:
... Few studies about bamboo naturally occurring mutants have been reported so far. Using an integrated anatomy, mathematics and genomics approach, we systematically characterized the cellular and molecular basis underlying the abnormal internode development of Pseudosasa japonica var. tsutsumiana, a stable variant with dwarf and swollen internodes that are caused by the compressed spiral growth and t ...
DOI:
10.1093/treephys/tpx129

19. Changes in the cerebellar cytoarchitecture of hibernating hedgehog Erinaceus europaeus L. (Mammalia): an immunocytochemical approach

Author:
E. Roda; M. G. Bottone; V. Insolia; S. Barni; G. Bernocchi
Source:
The European zoological journal 2017 v.84 no.1 pp. 496-511
ISSN:
2475-0263
Subject:
Erinaceus europaeus; ambient temperature; axons; calbindin; calcium; cerebellum; cerebral cortex; cytoskeleton; death; glutamate decarboxylase; glutamic acid; hibernation; homeostasis; immunohistochemistry; mammals; models; neurotransmitters; synaptic transmission
Abstract:
... Hibernation is an amazing animal strategy to survive when the environmental temperature is very low and food resources are scarce. Successful hibernation requires a variety of complex biological adaptations, in which the brain plays a central regulatory role. Currently, little information is available regarding the morphology and functional activity of specific neurons within the cerebellar cytoar ...
DOI:
10.1080/24750263.2017.1380722

20. Clinical and histopathological features of myofibrillar myopathy in Warmblood horses

Author:
S. J. Valberg; A. M. Nicholson; S. S. Lewis; R. A. Reardon; C. J. Finno
Source:
Equine veterinary journal 2017 v.49 no.6 pp. 739-745
ISSN:
0425-1644
Subject:
atrophy; biopsy; cytoskeleton; desmin; dystrophin; electron microscopy; exercise; gait; glycogen; histopathology; horses; lameness; muscle tissues; muscles; muscular diseases; myosin; protein aggregates
Abstract:
... BACKGROUND: To report a novel exertional myopathy, myofibrillar myopathy (MFM) in Warmblood (WB) horses. OBJECTIVES: To 1) describe the distinctive clinical and myopathic features of MFM in Warmblood horses and 2) investigate the potential inheritance of MFM in a Warmblood family. STUDY DESIGN: Retrospective selection of MFM cases and prospective evaluation of a Warmblood family. METHODS: Retrospe ...
DOI:
10.1111/evj.12702
PubMed:
28543538
PubMed Central:
PMC5640499

21. Coenzyme Q10 supplementation during in vitro maturation of bovine oocytes (Bos taurus) helps to preserve oocyte integrity after vitrification

Author:
M Ruiz‐Conca; M Vendrell; M Sabés‐Alsina; T Mogas; M Lopez‐Bejar
Source:
Reproduction in domestic animals 2017 v.52 Suppl S4 no. pp. 52-54
ISSN:
0936-6768
Subject:
antioxidants; cattle; coenzyme Q10; cooling; cortical granules; cytoskeleton; embryogenesis; exocytosis; mice; oocytes; plasma membrane; vitrification
Abstract:
... Oocyte vitrification causes less cell stress than slow cooling, but cytoskeletal and spindle alterations may occur affecting the oocyte competence. In vitro maturation (IVM) supplementation with different antioxidant molecules has been performed to attenuate this harmful stress. Coenzyme Q₁₀ (CoQ₁₀) supplementation has previously shown to have positive effects in bovine and mouse in vitro embryo d ...
DOI:
10.1111/rda.13056

22. Comparative proteomic analysis provides insight into 10-hydroxy-2-decenoic acid biosynthesis in honey bee workers

Author:
Xiao-Hui Yang; Shi-Fa Yang; Rui-Ming Wang
Source:
Amino acids 2017 v.49 no.7 pp. 1177-1192
ISSN:
0939-4451
Subject:
RNA interference; beta oxidation; biosynthesis; cytoskeleton; developmental stages; electron transfer; energy metabolism; fatty acid metabolism; fatty-acid synthase; flavoproteins; mandibular glands; protein metabolism; proteomics; secretion; worker honey bees
Abstract:
... 10-Hydroxy-2-decenoic acid (10-HDA) is the major compound produced from the mandibular glands (MGs) of honey bee workers. However, little information is available on the molecular mechanisms of 10-HDA biosynthesis. In our study, based on investigating the 10-HDA secretion pattern and the morphological characteristics of MGs from honey bee workers of different ages, a comparative proteomic analysis ...
DOI:
10.1007/s00726-017-2418-1

23. Comparative proteomics reveals the physiological differences between winter tender shoots and spring tender shoots of a novel tea (Camellia sinensis L.) cultivar evergrowing in winter

Author:
Shengjie Liu; Jiadong Gao; Zhongjian Chen; Xiaoyan Qiao; Hualin Huang; Baiyuan Cui; Qingfeng Zhu; Zhangyan Dai; Hualing Wu; Yayan Pan; Chengwei Yang; Jun Liu
Source:
BMC plant biology 2017 v.17 no.1 pp. 206
ISSN:
1471-2229
Subject:
Camellia sinensis; H+/K+-exchanging ATPase; H-transporting ATP synthase; amino acids; cultivars; cytoskeleton; desorption; flavonoids; histones; leaves; matrix-assisted laser desorption-ionization mass spectrometry; photosynthesis; plastocyanin; polyphenols; polysaccharides; profilins; protein synthesis; proteinases; proteomics; ribulose-bisphosphate carboxylase; shoots; spring; sugars; tea; temperature; transporters; winter
Abstract:
... BACKGROUND: A recently discovered tea [Camellia sinensis (L.) O. Kuntze] cultivar can generate tender shoots in winter. We performed comparative proteomics to analyze the differentially accumulated proteins between winter and spring tender shoots of this clonal cultivar to reveal the physiological basis of its evergrowing character during winter. RESULTS: We extracted proteins from the winter and ...
DOI:
10.1186/s12870-017-1144-x
PubMed:
29157222
PubMed Central:
PMC5697017

24. Critical role of Keratin 1 in maintaining epithelial barrier and correlation of its down-regulation with the progression of inflammatory bowel disease

Author:
Xiangqian Dong; Zichao Liu; Danfeng Lan; Junkun Niu; Jiarong Miao; Gang Yang; Fengrui Zhang; Yang Sun; Kunhua Wang; Yinglei Miao
Source:
Gene 2017 v.608 pp. 13-19
ISSN:
0378-1119
Subject:
Crohn disease; Western blotting; blood serum; colitis; colon; cytoskeleton; epithelium; human cell lines; immunohistochemistry; inflammation; interleukin-1beta; keratin; patients; reverse transcriptase polymerase chain reaction; small intestine; tight junctions
Abstract:
... Inflammatory bowel disease (IBD) is the result of a chronic intestinal inflammatory response which usually occurred in colon and small intestine. Keratins constitute the intermediate filament cytoskeleton in all epithelia. The present study was intended to explore the role of Keratin 1 (KRT1) in the progress of IBD. In normal intestinal tissue, the expression of KRT1 was detected by RT-PCR and Wes ...
DOI:
10.1016/j.gene.2017.01.015

25. Crosstalk of cell polarity signaling pathways

Author:
Tomáš Mazel
Source:
Protoplasma 2017 v.254 no.3 pp. 1241-1258
ISSN:
0033-183X
Subject:
cell movement; cell polarity; chemical elements; cytoskeleton; epithelium; guanosinetriphosphatase; lipid composition; lipids; messenger RNA; neurons; oocytes; organelles; pheromones; physiological transport; plasma membrane; receptors; signal transduction; yeasts
Abstract:
... Cell polarity, the asymmetric organization of cellular components along one or multiple axes, is present in most cells. From budding yeast cell polarization induced by pheromone signaling, oocyte polarization at fertilization to polarized epithelia and neuronal cells in multicellular organisms, similar mechanisms are used to determine cell polarity. Crucial role in this process is played by signal ...
DOI:
10.1007/s00709-017-1075-2

26. Curcumin alters the cytoskeleton and microtubule organization on trophozoites of Giardia lamblia

Author:
Filiberto Gutiérrez-Gutiérrez; Lissethe Palomo-Ligas; José Manuel Hernández-Hernández; Armando Pérez-Rangel; Rodrigo Aguayo-Ortiz; Alicia Hernández-Campos; Rafael Castillo; Sirenia González-Pozos; Rafael Cortés-Zárate; Mario Alberto Ramírez-Herrera; María Luisa Mendoza-Magaña; Araceli Castillo-Romero
Source:
Acta tropica 2017 pp. -
ISSN:
0001-706X
Subject:
Giardia lamblia; adhesion; adverse effects; binding sites; confocal microscopy; curcumin; flagellum; fluorescent antibody technique; giardiasis; microtubules; molecular dynamics; parasites; probability; scanning electron microscopy; staining; trophozoites; tubulin; turmeric; vinblastine
Abstract:
... Giardia lamblia is a worldwide protozoan responsible for a significant number of intestinal infections. There are several drugs for the treatment of giardiasis, but they often cause side effects. Curcumin, a component of turmeric, has antigiardial activity; however, the molecular target and mechanism of antiproliferative activity are not clear. The effects of curcumin on cellular microtubules have ...
DOI:
10.1016/j.actatropica.2017.04.027

27. Curdione attenuates thrombin-induced human platelet activation: β1-tubulin as a potential therapeutic target

Author:
Dongling Zhang; Wenhao Qiao; Yingli Zhao; Hui Fang; Dujuan Xu; Quan Xia
Source:
Fitoterapia 2017 v.116 pp. 106-115
ISSN:
0367-326X
Subject:
Curcuma aromatica; Western blotting; anticoagulants; bioinformatics; blood stasis; computer software; cytoskeleton; databases; essential oils; humans; integrins; medicinal plants; pain; platelet activation; protein synthesis; proteomics; rhizomes; sesquiterpenoids; signal transduction; thrombin
Abstract:
... Rhizoma Curcumae, the dry rhizomes derived from Curcuma aromatica Salisb., are a classical Chinese medicinal herb used to activate blood circulation, remove blood stasis and alleviate pain. Our previous study proved that curdione, a sesquiterpene compound isolated from the essential oil of Curcuma aromatica Salisb. can inhibit platelet activation suggesting its significant anticoagulant and antith ...
DOI:
10.1016/j.fitote.2016.11.016

28. Daboialectin, a C-type lectin from Russell's viper venom induces cytoskeletal damage and apoptosis in human lung cancer cells in vitro

Author:
Jigni Pathan; Sukanta Mondal; Angshuman Sarkar; Dibakar Chakrabarty
Source:
Toxicon 2017 v.127 pp. 11-21
ISSN:
0041-0101
Subject:
DNA damage; Daboia russelii; apoptosis; carcinoma; caspases; cell growth; cytoskeleton; cytotoxicity; guanosinetriphosphatase; humans; lectins; lung neoplasms; molecular weight; neoplasm cells; pro-apoptotic proteins; snakes; venoms
Abstract:
... ‘Daboialectin’, a low molecular weight C-type lectin (18.5 kDa) isolated from Russell's viper venom showed cytotoxic effects on human broncho-alveolar carcinoma derived (A549) cell lines. Daboialectin induced inhibition of A549 cell growth was time and concentration dependent with severe morphological changes by altering the functions of small GTPases such as Rac, Rho and cdc42. ROS induced DNA da ...
DOI:
10.1016/j.toxicon.2016.12.013

29. Deciphering the BAR code of membrane modulators

Author:
Ulrich Salzer; Julius Kostan; Kristina Djinović-Carugo
Source:
Cellular and molecular life sciences 2017 v.74 no.13 pp. 2413-2438
ISSN:
1420-682X
Subject:
cytoskeleton; lipids; post-translational modification; proteins
Abstract:
... The BAR domain is the eponymous domain of the “BAR-domain protein superfamily”, a large and diverse set of mostly multi-domain proteins that play eminent roles at the membrane cytoskeleton interface. BAR domain homodimers are the functional units that peripherally associate with lipid membranes and are involved in membrane sculpting activities. Differences in their intrinsic curvatures and lipid-b ...
DOI:
10.1007/s00018-017-2478-0
PubMed:
28243699
PubMed Central:
PMC5487894

30. Deep transcriptome profiling sheds light on key players in nucleus implantation induced immune response in the pearl oyster Pinctada martensii

Author:
Wei Wang; Yuyuan Wu; Qiannan Lei; Haiying Liang; Yuewen Deng
Source:
Fish & shellfish immunology 2017 v.69 pp. 67-77
ISSN:
1050-4648
Subject:
Pinctada; Toll-like receptors; cell adhesion molecules; cytoskeleton; databases; gene expression; gene expression regulation; immune response; immunosuppression; lectins; metabolism; oyster culture; oysters; pearls; peroxidase; ribosomes; sequence analysis; transcriptomics; unigenes
Abstract:
... Immunological rejection of the pearl oysters following nucleus implantation is a major issue limiting the successful rate of cultured pearls. To date, the molecular mechanism of immune tolerance during pearl formation in the pearl oysters is still largely unknown. Through the RNA sequencing platform and comparative transcriptomic analysis, we investigated the chronic gene expression changes at sev ...
DOI:
10.1016/j.fsi.2017.08.011

31. Differential gene expression in the endometrium reveals cytoskeletal and immunological genes in lactating dairy cows genetically divergent for fertility traits

Author:
Bruce Moran; Stephen T. Butler; Stephen G. Moore; David E. MacHugh; Christopher J. Creevey
Source:
Reproduction, fertility, and development 2017 v.29 no.2 pp. 274-282
ISSN:
1031-3613
Subject:
actin; biopsy; breeding; calving interval; cytoskeleton; dairy cows; endometrium; estrous cycle; gene expression; gene expression regulation; genes; genetic merit; immune response; lactating females; lactation; milk production; models; phenotypic variation; reproductive efficiency; reproductive performance; secretion; transcriptome; transportation; transporters; Ireland
Abstract:
... Profitable milk production in dairy cows requires good reproductive performance. Calving interval is a trait used to measure reproductive efficiency. Herein we used a novel lactating Holstein cow model of fertility that displayed genetic and phenotypic divergence in calving interval, a trait used to define reproductive performance using a national breeding index in Ireland. Cows had similar geneti ...
DOI:
10.1071/RD15128

32. Differential proteomic analysis of platelets suggested target-related proteins in rabbit platelets treated with Rhizoma Corydalis

Author:
Chun-Hong Li; Cen Chen; Qian Zhang; Chen-Ning Tan; Yuan-Jia Hu; Peng Li; Jian-Bo Wan; Gang Feng; Zhi-Ning Xia; Feng-Qing Yang
Source:
Pharmaceutical biology 2017 v.55 no.1 pp. 76-87
ISSN:
1744-5116
Subject:
Corydalis yanhusuo; adenosine diphosphate; arachidonic acid; blood platelets; calcium; cyclic GMP; cytoskeleton; enzyme-linked immunosorbent assay; ethanol; fluorescence; gene expression regulation; matrix-assisted laser desorption-ionization mass spectrometry; molecular chaperones; phosphatidylinositol 3-kinase; platelet aggregation; proteomics; rabbits; rhizomes; serotonin; signal transduction; thrombin; two-dimensional gel electrophoresis
Abstract:
... Context: Corydalis yanhusuo W.T. Wang (Papaveraceae) (Rhizoma Corydalis) showed inhibitory effects on rabbit platelet aggregation induced by ADP, thrombin (THR) or arachidonic acid (AA). Objective: This study separates and identifies the possible target-related platelet proteins and suggests possible signal cascades of RC antiplatelet aggregation. Materials and methods: Based on comparative proteo ...
DOI:
10.1080/13880209.2016.1229340
PubMed:
27653279
PubMed Central:
PMC7011957

33. Down-regulation of PAX2 promotes in vitro differentiation of podocytes from human CD34+ cells

Author:
Manne Mudhu Sunitha; Lokanathan Srikanth; Pasupuleti Santhosh Kumar; Chodimella Chandrasekhar; Potukuchi Venkata Gurunadha Krishna Sarma
Source:
Cell and tissue research 2017 v.370 no.3 pp. 477-488
ISSN:
0302-766X
Subject:
adults; agarose; blood; cell movement; cytoskeleton; diaphragm; enzyme activity; glomerular filtration rate; glycoproteins; humans; immunocytochemistry; kidney cells; kidney diseases; monoclonal antibodies; phosphorylation; retinoic acid; staining; stem cells; tyrosine
Abstract:
... Podocytes are major kidney cells that help in glomerular filtration and any damage or loss is a major event in the progression of kidney diseases. Understanding podocytes development will help in designing therapeutic strategies against these renal diseases. Therefore, in vitro generation of podocytes from adult hematopoietic CD34⁺ stem cells is explored in the present study. Apheretically, isolat ...
DOI:
10.1007/s00441-017-2680-2

34. Drosophila tensin plays an essential role in cell migration and planar polarity formation during oogenesis by mediating integrin-dependent extracellular signals to actin organization

Author:
In Jun Cha; Jang Ho Lee; Kyoung Sang Cho; Sung Bae Lee
Source:
Biochemical and biophysical research communications 2017 pp. -
ISSN:
0006-291X
Subject:
Drosophila; actin; animal ovaries; cell movement; cytoskeleton; eggs; hatching; integrins; mutants; mutation; oogenesis; phenotype; receptors
Abstract:
... Oogenesis in Drosophila involves very dynamic cellular changes such as cell migration and polarity formation inside an ovary during short period. Previous studies identified a number of membrane-bound receptors directly receiving certain types of extracellular inputs as well as intracellular signalings to be involved in the regulation of these dynamic cellular changes. However, yet our understandi ...
DOI:
10.1016/j.bbrc.2017.01.183

35. Effect of vitrification on the mRNA transcriptome of bovine oocytes

Author:
N Wang; C‐Y Li; H‐B Zhu; H‐S Hao; H‐Y Wang; C‐L Yan; S‐J Zhao; W‐H Du; D Wang; Y Liu; Y‐W Pang; X‐M Zhao
Source:
Reproduction in domestic animals 2017 v.52 no.4 pp. 531-541
ISSN:
0936-6768
Subject:
calcium; cattle; cell cycle; cytoskeleton; gene expression; gene expression regulation; gene ontology; genes; glucose; ions; messenger RNA; metabolism; mitochondria; oocytes; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ribosomal proteins; sequence analysis; transcription (genetics); transcriptome; transmembrane proteins; vitrification
Abstract:
... Vitrification has been shown to decrease the developmental capacity of mammalian oocytes, and this is closely associated with the abnormal mRNA expressions of vitrified oocytes. However, the effect of vitrification on transcriptional machinery of oocytes examined by RNA sequencing (RNA‐seq) has yet to be defined. In the present study, the mRNA transcriptomes of fresh and vitrified bovine oocytes w ...
DOI:
10.1111/rda.12942

36. Effects of ovarian disaggregation on adult murine follicle yield and viability

Author:
Fiona Young; John Drummond; Emma Akers; Louise Bartle; David Kennedy; Mohammad Asaduzzaman
Source:
Reproduction, fertility, and development 2017 v.29 no.12 pp. 2400-2410
ISSN:
1031-3613
Subject:
4',6-diamidino-2-phenylindole; adults; antibodies; collagenase; cytoskeleton; fluorescein; genetic markers; granulosa cells; mice; mitochondria; rapid methods; screening; staining; tubulin; viability
Abstract:
... Follicles are isolated from ovaries for numerous reasons, including IVM, but adult murine yields are <2 folliclesmg-1. The aim of the present study was to optimise ovarian disaggregation and develop methods applicable to the rapid screening of follicle viability. Ovaries from adult mice (n=7) were halved and disaggregated mechanically, or by using collagenase IV (Col-IV; 590UmL-1) or animal origin ...
DOI:
10.1071/RD16398

37. Effects of vitrification of cumulus-enclosed porcine oocytes at the germinal vesicle stage on cumulus expansion, nuclear progression and cytoplasmic maturation

Author:
Ruth Appeltant; Tamás Somfai; Elisa C. S. Santos; Thanh Quang Dang-Nguyen; Takashi Nagai; Kazuhiro Kikuchi
Source:
Reproduction, fertility, and development 2017 v.29 no.12 pp. 2419-2429
ISSN:
1031-3613
Subject:
actin; adenosine triphosphate; apoptosis; cell nucleolus; cooling; cryoprotectants; embryogenesis; ethylene glycol; germinal vesicle; glutathione; meiosis; messenger RNA; progeny; propylene glycol; swine; toxicity; vitrification
Abstract:
... Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus–oocyte complexes (COCs) and control COCs (no cryoprotectant treatment ...
DOI:
10.1071/RD16386

38. Enteric glial cells are susceptible to Clostridium difficile toxin B

Author:
Katia Fettucciari; Pamela Ponsini; Davide Gioè; Lara Macchioni; Camilla Palumbo; Elisabetta Antonelli; Stefano Coaccioli; Vincenzo Villanacci; Lanfranco Corazzi; Pierfrancesco Marconi; Gabrio Bassotti
Source:
Cellular and molecular life sciences 2017 v.74 no.8 pp. 1527-1551
ISSN:
1420-682X
Subject:
Clostridium difficile; apoptosis; cell cycle checkpoints; colitis; cyclins; cytoskeleton; cytotoxicity; diarrhea; enterocytes; glucosylation; guanosinetriphosphatase; homeostasis; inflammation; interferon-gamma; neuroglia; neurons; pathogenesis; toxins; tumor necrosis factor-alpha; virulence
Abstract:
... Clostridium difficile causes nosocomial/antibiotic-associated diarrhoea and pseudomembranous colitis. The major virulence factors are toxin A and toxin B (TcdB), which inactivate GTPases by monoglucosylation, leading to cytopathic (cytoskeleton alteration, cell rounding) and cytotoxic effects (cell-cycle arrest, apoptosis). C. difficile toxins breaching the intestinal epithelial barrier can act on ...
DOI:
10.1007/s00018-016-2426-4

39. Enterolactone alters FAK-Src signaling and suppresses migration and invasion of lung cancer cell lines

Author:
Shireen Chikara; Kaitlin Lindsey; Pawel Borowicz; Melpo Christofidou-Solomidou; Katie M. Reindl
Source:
BMC complementary and alternative medicine 2017 v.17 no.1 pp. 30
ISSN:
1472-6882
Subject:
Western blotting; actin; alternative medicine; antineoplastic activity; bioactive compounds; bromides; cell movement; cytoskeleton; drug therapy; fluorescence microscopy; focal adhesions; gene expression; genes; humans; immunocytochemistry; lignans; lung neoplasms; messenger RNA; metastasis; neoplasm cells; phosphorylation; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; tissue repair; tissues; toxicity
Abstract:
... BACKGROUND: Systemic toxicity of chemotherapeutic agents and the challenges associated with targeting metastatic tumors are limiting factors for current lung cancer therapeutic approaches. To address these issues, plant-derived bioactive components have been investigated for their anti-cancer properties because many of these agents are non-toxic to healthy tissues. Enterolactone (EL) is a flaxseed ...
DOI:
10.1186/s12906-016-1512-3
PubMed:
28068967
PubMed Central:
PMC5223372

40. Expansion and cryopreservation of porcine and human corneal endothelial cells

Author:
Leah A. Marquez-Curtis; Locksley E. McGann; Janet A.W. Elliott
Source:
Cryobiology 2017 v.77 pp. 1-13
ISSN:
0011-2240
Subject:
actin; basement membrane; blindness; cooling; cornea; cryopreservation; cytoskeleton; dimethyl sulfoxide; endothelium; human umbilical vein endothelial cells; humans; medicine; nitrogen; starch; swine; thawing; tight junctions; tissue engineering
Abstract:
... Impairment of the corneal endothelium causes blindness that afflicts millions worldwide and constitutes the most often cited indication for corneal transplants. The scarcity of donor corneas has prompted the alternative use of tissue-engineered grafts which requires the ex vivo expansion and cryopreservation of corneal endothelial cells. The aims of this study are to culture and identify the condi ...
DOI:
10.1016/j.cryobiol.2017.04.012

41. Fasciclin-calcareous corpuscle binary complex mediated protein-protein interactions in Taenia solium metacestode

Author:
Chun-Seob Ahn; Jeong-Geun Kim; Young-An Bae; Seon-Hee Kim; Joo-Ho Shin; Yichao Yang; Insug Kang; Yoon Kong
Source:
Parasites & vectors 2017 v.10 no.1 pp. 438
ISSN:
1756-3305
Subject:
Taenia solium; actin; adhesion; amino acid sequences; amino acids; bioactive compounds; bladder; carbohydrate metabolism; cell movement; cytoskeleton; disease course; exons; glyceraldehyde-3-phosphate dehydrogenase; innexins; introns; ligands; nervous system diseases; neurocysticercosis; phosphoglycerate kinase; phosphopyruvate hydratase; polypeptides; post-translational modification; protein-protein interactions; proteome; proteomics; recombinant proteins; sequence analysis
Abstract:
... BACKGROUND: Neurocysticercosis (NC) caused by Taenia solium metacestode (TsM) is a serious neurological disease of global concern. Diverse bioactive molecules involved in the long-term survival of TsM might contribute to disease progression. Fasciclin (Fas) is an extracellular protein that mediates adhesion, migration and differentiation of cells by interacting with other molecules. We hypothesize ...
DOI:
10.1186/s13071-017-2359-2
PubMed:
28931431
PubMed Central:
PMC5606126

42. Functional characterization of lysophosphatidic acid receptor 1 mutants identified in rat cancer tissues

Author:
Shoichi Ishii; Toshifumi Tsujiuchi; Nobuyuki Fukushima
Source:
Biochemical and biophysical research communications 2017 pp. -
ISSN:
0006-291X
Subject:
calcium; cyclic AMP; cytoskeleton; endoplasmic reticulum; lipids; missense mutation; mutants; neoplasm cells; neoplasms; rats; receptors; tissues
Abstract:
... Lysophosphatidic acid (LPA), an extracellular lipid mediator, exerts various cellular effects through activation of LPA receptors, LPA1–LPA6, in many types of cells including cancer cells. We recently found several missense mutations of Lpar1 in rat cancer tissues. One of these mutations is located at the extracellular tip of the seventh transmembrane domain of LPA1, and another three mutations ar ...
DOI:
10.1016/j.bbrc.2017.03.118

43. Gill transcriptomes reveal involvement of cytoskeleton remodeling and immune defense in ammonia stress response in the banana shrimp Fenneropenaeus merguiensis

Author:
Wei Wang; Shiping Yang; Chenggui Wang; Lili Shi; Hui Guo; Siuming Chan
Source:
Fish & shellfish immunology 2017 v.71 pp. 319-328
ISSN:
1050-4648
Subject:
Fenneropenaeus merguiensis; ammonia; breeding; cytoskeleton; fisheries; gene expression regulation; gene ontology; genetic markers; immune response; loci; microsatellite repeats; sequence analysis; shrimp; stress response; stress tolerance; transcriptome; transcriptomics; unigenes
Abstract:
... The banana shrimp, Fenneropenaeus merguiensis, is an important fishery species in the Indo-West Pacific region. As the shrimp is very sensitive to stressors such as ammonia stress in water, understanding the molecular mechanisms of stress tolerance in F. merguiensis is of pivotal importance for improving its farming performance. In the current study, by using the RNA sequencing platform and compar ...
DOI:
10.1016/j.fsi.2017.10.028

44. Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics

Author:
Gonzalo P. Solis; Oleksii Bilousov; Alexey Koval; Anne-Marie Lüchtenborg; Chen Lin; Vladimir L. Katanaev
Source:
Cell 2017 v.170 pp. 939-955.e24
ISSN:
0092-8674
Subject:
G-protein coupled receptors; cytoskeleton; feeds; guanosinetriphosphatase; insects; mammals; neurites; plasma membrane; tissues
Abstract:
... To form protrusions like neurites, cells must coordinate their induction and growth. The first requires cytoskeletal rearrangements at the plasma membrane (PM), the second requires directed material delivery from cell’s insides. We find that the Gαo-subunit of heterotrimeric G proteins localizes dually to PM and Golgi across phyla and cell types. The PM pool of Gαo induces, and the Golgi pool feed ...
DOI:
10.1016/j.cell.2017.07.015

45. Hydroxyapatite as a Vehicle for the Selective Effect of Superparamagnetic Iron Oxide Nanoparticles against Human Glioblastoma Cells

Author:
Pernal Sebastian; Wu Victoria M.; Uskoković Vuk
Source:
ACS applied materials & interfaces 2017 v.9 no.45 pp. 39283-39302
ISSN:
1944-8252
Subject:
biocompatibility; bones; brain neoplasms; cell viability; fibroblasts; humans; hydroxyapatite; kidneys; magnetic fields; mice; microfilaments; models; nanocomposites; nanohybrids; nanoparticles; neoplasm cells; risk; stem cells; temperature; toxicity
Abstract:
... Despite the early promises of magnetic hyperthermia (MH) as a method for treating cancer, it has been stagnating in the past decade. Some of the reasons for the low effectiveness of superparamagnetic nanoparticles (SPIONs) in MH treatments include (a) low uptake in cancer cells; (b) generation of reactive oxygen species that cause harm to the healthy cells; (c) undeveloped targeting potential; and ...
DOI:
10.1021/acsami.7b15116
PubMed:
29058880
PubMed Central:
PMC5796653

46. Identification and characterization of adhesion proteins in lactobacilli targeting actin as receptor

Author:
Zhen Peng; Rudi F. Vogel; Matthias A. Ehrmann; Tao Xiong
Source:
Molecular and cellular probes 2017 pp. -
ISSN:
0890-8508
Subject:
Lactobacillus; actin; adhesion; bacterial adhesion; bacterial colonization; chaperonins; cytoskeleton; electrophoresis; glucose-6-phosphate isomerase; lactic acid bacteria; lysine; pathogens; phosphoglycerate kinase; pyruvate kinase
Abstract:
... Actin as the main constitution of cytoskeleton in host cells plays an important role in mediating bacterial colonization. To identify the actin-binding proteins in Lactobacillus (L.) paracasei, L. plantarum, and L. brevis, actin immobilized to 24-well plate was used to probe adhesion proteins. Five adhesion proteins were identified and characterized by electrophoresis and LC-MS/MS: pyruvate kinase ...
DOI:
10.1016/j.mcp.2017.08.002

47. Identification of motility-associated progesterone-responsive differentially phosphorylated proteins

Author:
V. Sagare-Patil; D. Modi
Source:
Reproduction, fertility, and development 2017 v.29 no.6 pp. 1115-1129
ISSN:
1031-3613
Subject:
cytoskeleton; dephosphorylation; flagellum; humans; progesterone; protein phosphorylation; proteins; sperm motility; spermatozoa; tyrosine
Abstract:
... Progesterone is one of the regulators of sperm motility and hyperactivation. In human spermatozoa, the effects of progesterone are thought to be mediated by protein phosphorylation. In the present study, we identified 22 proteins that are differentially phosphorylated (12 phosphorylated and 10 dephosphorylated) by progesterone in human spermatozoa. Functionally, the differentially phosphorylated p ...
DOI:
10.1071/RD15492

48. Impact of immobilizing of low molecular weight hyaluronic acid within gelatin-based hydrogel through enzymatic reaction on behavior of enclosed endothelial cells

Author:
Mehdi Khanmohammadi; Shinji Sakai; Masahito Taya
Source:
International journal of biological macromolecules 2017 v.97 pp. 308-316
ISSN:
0141-8130
Subject:
cell movement; cytoskeleton; dose response; enzymatic reactions; horseradish; human umbilical vein endothelial cells; hyaluronic acid; hydrocolloids; molecular weight; morphogenesis; tissue engineering; tissues
Abstract:
... The hydrogels having the ability to promote migration and morphogenesis of endothelial cells (ECs) are useful for fabricating vascularized dense tissues in vitro. The present study explores the immobilization of low molecular weight hyaluronic acid (LMWHA) derivative within gelatin-based hydrogel to stimulate migration of ECs. The LMWHA derivative possessing phenolic hydroxyl moieties (LMWHA-Ph) w ...
DOI:
10.1016/j.ijbiomac.2016.12.088

49. Influence of Postmortem Aging of Fresh Pork Loin on Instrumental Tenderness and Abundance of a Soluble Desmin Degradation Product

Author:
A. C. Outhouse; K. J. Prusa; C. A. Fedler; E. M. Steadham; M. D. Schulte; E. Huff-Lonergan; S. M. Lonergan
Source:
Meat and muscle biology 2017 v.1 no.3 pp. 99
ISSN:
2575-985X
Subject:
EDTA (chelating agent); chops; color; cytoskeleton; data analysis; desmin; fractionation; gels; immunoblotting; ionic strength; liquid nitrogen; loins (meat cut); longissimus muscle; marbling; meat aging; meat tenderness; pH; pork; pork quality; prediction; proteinases; raw meat; striated muscle
Abstract:
... ObjectivesIt is well understood that aging fresh pork loins will improve tenderness. The explanation for this phenomenon is degradation of myofibrillar, cytoskeletal, and intermediate filament proteins by endogenous proteolytic enzymes. Recently, the abundance of a desmin fragment in the sarcoplasmic fraction of aged pork has been linked to differences in pork tenderness. The objective of this exp ...
DOI:
10.221751/rmc2017.094

50. Light-Controlled Membrane Mechanics and Shape Transitions of Photoswitchable Lipid Vesicles

Author:
Pernpeintner Carla; Frank James A.; Urban Patrick; Roeske Christian R.; Pritzl Stefanie D.; Trauner Dirk; Lohmüller Theobald
Source:
Langmuir 2017 v.33 no.16 pp. 4083-4089
ISSN:
1520-5827
Subject:
cytoskeleton; energy; irradiation; mechanical properties; models; phosphatidylcholines; photoisomerization; plasma membrane; ultraviolet radiation
Abstract:
... Giant unilamellar vesicles (GUVs) represent a versatile model system to emulate the fundamental properties and functions associated with the plasma membrane of living cells. Deformability and shape transitions of lipid vesicles are closely linked to the mechanical properties of the bilayer membrane itself and are typically difficult to control under physiological conditions. Here, we developed a p ...
DOI:
10.1021/acs.langmuir.7b01020