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- Chen, Ying-Chou, et al. Show all 6 Authors
- Molecular cell 2017 v.68 no.1 pp. 247-257.e5
- Parkinson disease; RNA; cell death; gene expression regulation; gene overexpression; gene regulatory networks; genes; humans; models; neurons; phenotype; screening; toxicity; transcription (genetics); transcription factors; yeasts
- ... The genome-wide perturbation of transcriptional networks with CRISPR-Cas technology has primarily involved systematic and targeted gene modulation. Here, we developed PRISM (Perturbing Regulatory Interactions by Synthetic Modulators), a screening platform that uses randomized CRISPR-Cas transcription factors (crisprTFs) to globally perturb transcriptional networks. By applying PRISM to a yeast mod ...
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- Chen, Ying, et al. Show all 6 Authors
- Field crops research 2017 v.200 pp. 58-70
- Oryza sativa; absorption; canopy; carbon dioxide; carboxylation; chlorophyll; electron transfer; field experimentation; genes; leaves; light harvesting complex; membrane proteins; mesophyll; models; mutants; nitrogen content; nutrient use efficiency; photosystem II; ribulose-bisphosphate carboxylase; rice; solar radiation; stomata; thylakoids; transmittance
- ... The net photosynthesis and photosynthetic N use efficiency (PNUE) of a rice mutant with reduced chlorophyll synthesis were much higher than those of its wild type (WT). To understand the physiological mechanism, we conducted a model analysis and pot and field experiments under different N rates. Both the model analysis and the experiments showed that low chlorophyll content did not impede the deve ...
- Chen, Ying-Han, et al. Show all 9 Authors
- Molecular cell 2017 v.66 no.5 pp. 635-647.e7
- cell viability; cytokines; genes; immune response; immunotherapy; inflammation; interferon-gamma; melanoma; models; pathogens; phenotypic plasticity; phosphatidylserines; surveys; transcription (genetics)
- ... Immune cells constantly survey the host for pathogens or tumors and secrete cytokines to alert surrounding cells of these threats. In vivo, activated immune cells secrete cytokines for several hours, yet an acute immune reaction occurs over days. Given these divergent timescales, we addressed how cytokine-responsive cells translate brief cytokine exposure into phenotypic changes that persist over ...