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- Chen, Ying-Chou, et al. Show all 6 Authors
- Molecular cell 2017 v.68 no.1 pp. 247-257.e5
- Parkinson disease; RNA; cell death; gene expression regulation; gene overexpression; gene regulatory networks; genes; humans; models; neurons; phenotype; screening; toxicity; transcription (genetics); transcription factors; yeasts
- ... The genome-wide perturbation of transcriptional networks with CRISPR-Cas technology has primarily involved systematic and targeted gene modulation. Here, we developed PRISM (Perturbing Regulatory Interactions by Synthetic Modulators), a screening platform that uses randomized CRISPR-Cas transcription factors (crisprTFs) to globally perturb transcriptional networks. By applying PRISM to a yeast mod ...
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