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1. Inga vera trypsin inhibitor interferes in the proteolytic activity and nutritional physiology of Ephestia kuehniella larvae

Author:
Bezerra, Cézar da Silva; Oliveira, Carolina Turatti; Macedo, Maria Lígia Rodrigues
Source:
Entomologia experimentalis et applicata 2017 v.165 no.2-3 pp. 109-119
ISSN:
0013-8703
Subject:
trypsin inhibitors,   etc   ; Ephestia kuehniella; Inga vera; bioassays; chymotrypsin; digestion; frass; hydrolysis; ingestion; insect physiology; insects; larvae; midgut; pest control; proteolysis; seeds; trypsin;     Show all 17 Subjects
Abstract:
... Plant peptidase inhibitors provide plants with a defense strategy to inhibit insect digestive enzymes and have been studied as an alternative strategy for pest control as they interfere in normal insect physiology. We evaluated the effects of ingestion of the trypsin inhibitor from Inga vera Willd. (Fabaceae) seeds on the nutritional and digestive physiology of Ephestia kuehniella (Zeller) (Lepido ...
DOI:
10.1111/eea.12605

2. Performance of mechanical co-extraction of Jatropha curcas L. kernels with rapeseed, maize or soybean with regard to oil recovery, press capacity and product quality

Author:
Romuli, Sebastian; Karaj, Shkelqim; Latif, Sajid; Müller, Joachim
Source:
Industrial crops and products 2017 v.104 pp. 81-90
ISSN:
0926-6690
Subject:
trypsin inhibitors,   etc   ; Jatropha curcas; acid value; additives; antinutritional factors; ash content; carbon; corn; corn oil; crude protein; energy requirements; free fatty acids; lipid content; lysine; mixing; nitrogen; pepsin; phytic acid; potassium hydroxide; pressing; product quality; protein sources; rapeseed; response surface methodology; seeds; soybean oil; soybeans; specific energy; temperature; texture; trypsin;     Show all 31 Subjects
Abstract:
... Jatropha curcas L. shelled seeds (kernels) have been gaining attention as protein source due to their high protein content. However, due to the soft texture, screw pressing the kernels resulted in a low performance, which was indicated by high residual oil content in the press cake. In order to overcome the low oil recovery from the extraction process, J. curcas kernels blended with rapeseed, maiz ...
DOI:
10.1016/j.indcrop.2017.03.035

3. Microgramma vacciniifolia (Polypodiaceae) fronds contain a multifunctional lectin with immunomodulatory properties on human cells

Author:
de Siqueira Patriota, Leydianne Leite; Procópio, Thamara Figueiredo; de Santana Brito, Jéssica; Sebag, Virginie; de Oliveira, Ana Patrícia Silva; de Araújo Soares, Ana Karine; Moreira, Leyllane Rafael; de Albuquerque Lima, Thâmarah; Soares, Tatiana; da Silva, Túlio Diego; Paiva, Patrícia Maria Guedes; de Lorena, Virgínia Maria Barros; de Melo, Cristiane Moutinho Lagos; de Albuquerque, Lidiane Pereira; Napoleão, Thiago Henrique
Source:
International journal of biological macromolecules 2017 v.103 pp. 36-46
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; Microgramma; RNA-binding proteins; T-lymphocytes; cytotoxicity; fronds; glycoproteins; heat stability; hemagglutination; humans; immunomodulators; interferon-gamma; interleukin-10; interleukin-6; lectins; nitric oxide; pH; sequence homology; trypsin; tumor necrosis factor-alpha;     Show all 20 Subjects
Abstract:
... In this study, we report the purification and characterization of a multifunctional lectin (MvFL) from Microgramma vacciniifolia fronds as well as its immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). MvFL (pI 4.51; 54kDa) is a glycoprotein able to inhibit trypsin activity and that has sequence similarities (32% coverage) with a plant RNA-binding protein. Hemagglutin ...
DOI:
10.1016/j.ijbiomac.2017.05.037

4. Peptidase compensation in the digestive system of whiteleg shrimp Penaeus vannamei against dietary Kunitz‐type soybean trypsin inhibitor

Author:
Maytorena‐Verdugo, C.I.; Córdova‐Murueta, J.H.; García‐Carreño, F.L.
Source:
Aquaculture nutrition 2017 v.23 no.5 pp. 1095-1103
ISSN:
1353-5773
Subject:
trypsin inhibitors,   etc   ; Litopenaeus vannamei; bioassays; chymotrypsin; enzyme activity; feces; proteolysis; serine; shrimp; soybean meal; soybeans; trypsin;     Show all 12 Subjects
Abstract:
... The aim of this study was to assess the regulatory process of digestive peptidases of crustaceans in the presence of soybean trypsin inhibitor (SBTI). This naturally occurring inhibitor in soybean meal was used to inhibit the activity of digestive serine peptidases of the whiteleg shrimp Litopenaeus vannamei. In vitro, SBTI inhibited the total proteolytic activity and chymotrypsin activity by 65%. ...
DOI:
10.1111/anu.12477

5. Application and bioactive properties of CaTI, a trypsin inhibitor from Capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells

Author:
Silva, Marciele S; Ribeiro, Suzanna FF; Taveira, Gabriel B; Rodrigues, Rosana; Fernandes, Katia VS; Carvalho, André O; Vasconcelos, Ilka Maria; Mello, Erica Oliveira; Gomes, Valdirene M
Source:
Journal of the science of food and agriculture 2017 v.97 no.11 pp. 3790-3801
ISSN:
0022-5142
Subject:
trypsin inhibitors,   etc   ; Capsicum annuum; Fusarium oxysporum; Glomerella cingulata; antifungal properties; antimicrobial peptides; enzyme activity; fluorescein; hot peppers; hyphae; isothiocyanates; mechanism of action; nitric oxide; oxidative stress; plant pathogenic fungi; reactive oxygen species; seeds; trypsin;     Show all 18 Subjects
Abstract:
... BACKGROUND: During the last few years, a growing number of antimicrobial peptides have been isolated from plants and particularly from seeds. Recent results from our laboratory have shown the purification of a new trypsin inhibitor, named CaTI, from chilli pepper (Capsicum annuum L.) seeds. This study aims to evaluate the antifungal activity and mechanism of action of CaTI on phytopathogenic fungi ...
DOI:
10.1002/jsfa.8243

6. Trypsin inhibitor screening in traditional Chinese medicine by using an immobilized enzyme microreactor in capillary and molecular docking study

Author:
Cheng, Mengxia; Chen, Zilin
Source:
Journal of separation science 2017 v.40 no.15 pp. 3168-3174
ISSN:
1615-9306
Subject:
trypsin inhibitors,   etc   ; Oriental traditional medicine; baicalin; capillary electrophoresis; enzyme kinetics; immobilized enzymes; molecular models; screening; trypsin;     Show all 9 Subjects
Abstract:
... A trypsin immobilized enzyme microreactor was successfully prepared in capillary for studying enzyme kinetics of trypsin and online screening of trypsin inhibitors from traditional Chinese medicine through capillary electrophoresis. Trypsin was immobilized on the inner wall at the inlet of the capillary treated with polydopamine. The rest of the capillary was used as a separation channel. The para ...
DOI:
10.1002/jssc.201700419

7. A case of neofunctionalization of a Putranjiva roxburghii PNP protein to trypsin inhibitor by disruption of PNP-UDP domain through an insert containing inhibitory site

Author:
Verma, Preeti; Patel, Girijesh K.; Kar, Bibekananda; Sharma, Ashwani K.
Source:
Plant science 2017 v.260 pp. 19-30
ISSN:
0168-9452
Subject:
trypsin inhibitors,   etc   ; Putranjiva roxburghii; active sites; divergent evolution; enzyme activity; guanosine; plant proteins; sequence analysis; storage proteins; trypsin;     Show all 10 Subjects
Abstract:
... The attainment of new function by a protein is achieved through convergent/divergent evolution. In present work, the sequence analysis of a 34kDa protein from Putranjiva roxburghii, earlier reported as a potent trypsin inhibitor, showed resemblance to some of the wound inducible and vegetative storage proteins. A detailed sequence analysis revealed that these proteins belong to PNP-UDP family. In ...
DOI:
10.1016/j.plantsci.2017.03.013

8. A trypsin inhibitor purified from Cassia leiandra seeds has insecticidal activity against Aedes aegypti

Author:
Dias, Lucas P.; Oliveira, Jose T.A.; Rocha-Bezerra, Lady C.B.; Sousa, Daniele O.B.; Costa, Helen P.S.; Araujo, Nadine M.S.; Carvalho, Ana F.U.; Tabosa, Pedro M.S.; Monteiro-Moreira, Ana C.O.; Lobo, Marina D.P.; Moreno, Frederico B.M.B.; Rocha, Bruno A.M.; Lopes, José L.S.; Beltramini, Leila M.; Vasconcelos, Ilka M.
Source:
Process biochemistry 2017 v.57 pp. 228-238
ISSN:
1359-5113
Subject:
trypsin inhibitors,   etc   ; Aedes aegypti; Cassia; acute toxicity; amino acid sequences; cattle; chromatography; dithiothreitol; glycoproteins; inhibitory concentration 50; insecticidal properties; instars; larvae; lethal concentration 50; mass spectrometry; midgut; mortality; pH; polypeptides; seeds; temperature; trypsin;     Show all 22 Subjects
Abstract:
... A trypsin inhibitor from Cassia leiandra seeds, named ClTI, was purified, characterized, and its insecticidal activity against Aedes aegypti evaluated. ClTI was purified by DEAE-Cellulose and trypsin-Sepharose 4B chromatography, with a 15.5-fold purification and 2.4% yield. ClTI is composed of a 19,484Da polypeptide chain as revealed by mass spectrometry, it is not a glycoprotein, its amino acid s ...
DOI:
10.1016/j.procbio.2017.03.015

9. Purification and antiparasitic activity of a few legume serine proteinase inhibitors: Effect on erythrocyte invasion, schizont rupture and proteolytic processing of the Plasmodium falciparum AMA1 protein

Author:
Bhattacharyya, Arindam; Chitnis, Chetan E.; Babu, C.R.
Source:
Process biochemistry 2017 v.57 pp. 207-218
ISSN:
1359-5113
Subject:
trypsin inhibitors,   etc   ; Archidendron; Derris trifoliata; Plasmodium falciparum; additive effect; adverse effects; antigens; antimalarials; antiparasitic properties; chymotrypsin; erythrocytes; high performance liquid chromatography; inhibitory concentration 50; merozoites; parasites; proteins; proteolysis; soybeans; synergism; trypsin;     Show all 20 Subjects
Abstract:
... Legume proteinase inhibitors (PI/s) abrogate proteolytic activity of proteins and cause adverse effects on growth. In this study, two legume PIs – Archidendron ellipticum Trypsin Inhibitor (AeTI) and Derris trifoliata Trypsin Chymotrypsin Inhibitor (DtTCI) were purified and used along with standard, Soybean Trypsin Inhibitor (STI), either singly or in combination, as antiplasmodial agents against ...
DOI:
10.1016/j.procbio.2017.03.011

10. Litopenaeus vannamei digestive metallo peptidases compensate for anti-nutritional SBTI in feed

Author:
Maytorena-Verdugo, Claudia I.; Córdova-Murueta, Julio H.; García-Carreño, Fernando L.
Source:
Aquaculture 2017 v.473 pp. 508-512
ISSN:
0044-8486
Subject:
trypsin inhibitors,   etc   ; Litopenaeus vannamei; chymotrypsin; homeostasis; ingestion; phenotype; proteolysis; shrimp; soybeans; trypsin;     Show all 10 Subjects
Abstract:
... The aim of this work was to study mechanisms of homeostasis of the digestive system of the whiteleg shrimp Litopenaeus vannamei when fed anti-nutritional soybean trypsin inhibitor (SBTI). In a previous work, we tested if the shrimp could respond against SBTI in feed with the increase in the digestive proteolytic activity. This time we tested the time of response against the inhibitor and also how ...
DOI:
10.1016/j.aquaculture.2017.02.023

11. Aggregation of trypsin and trypsin inhibitor by Al cation

Author:
Chanphai, P.; Kreplak, L.; Tajmir-Riahi, H.A.
Source:
Journal of photochemistry and photobiology 2017 v.169 pp. 7-12
ISSN:
1011-1344
Subject:
trypsin inhibitors,   etc   ; Fourier transform infrared spectroscopy; aluminum; atomic force microscopy; cations; hydrogen bonding; neurodegenerative diseases; proteins; t-test; thermodynamics; trypsin; van der Waals forces;     Show all 12 Subjects
Abstract:
... Al cation may trigger protein structural changes such as aggregation and fibrillation, causing neurodegenerative diseases. We report the effect of Al cation on the solution structures of trypsin (try) and trypsin inhibitor (tryi), using thermodynamic analysis, UV–Visible, Fourier transform infrared (FTIR) spectroscopic methods and atomic force microscopy (AFM). Thermodynamic parameters showed Al-p ...
DOI:
10.1016/j.jphotobiol.2017.02.018

12. Major trypsin like‐serine proteinases from albacore tuna (Thunnus alalunga) spleen: Biochemical characterization and the effect of extraction media

Author:
Poonsin, Tanchanok; Sripokar, Pakteera; Benjakul, Soottawat; Simpson, Benjamin K.; Visessanguan, Wonnop; Klomklao, Sappasith
Source:
Journal of food biochemistry 2017 v.41 no.2
ISSN:
0145-8884
Subject:
trypsin inhibitors,   etc   ; Thunnus alalunga; buffers; detergents; electrophoresis; fluorides; leather; molecular weight; pH; pollution; processing waste; proteolysis; silk; sodium chloride; sodium phosphate; soybeans; spleen; temperature; trypsin; tuna;     Show all 20 Subjects
Abstract:
... This investigation aimed to characterize the proteinases and to study the effect of extraction media on proteinases recovery from albacore tuna spleen. Optimal activity of splenic extract was at pH 9.5 and 55°C. The enzyme was stable in a wide pH range of 6.0–10.0 but unstable at the temperatures greater than 50°C for 30–120 min. The proteolytic activity was strongly inhibited by soybean trypsin i ...
DOI:
10.1111/jfbc.12323

13. Peanut Seed Cultivars with Contrasting Resistance to Aspergillus parasiticus Colonization Display Differential Temporal Response of Protease Inhibitors

Author:
Müller, Virginia; Bonacci, Gustavo; Batthyany, Carlos; Amé, María V.; Carrari, Fernando; Gieco, Jorge; Asis, Ramón
Source:
Phytopathology 2017 v.107 no.4 pp. 474-482
ISSN:
0031-949X
Subject:
trypsin inhibitors,   etc   ; Aspergillus parasiticus; aflatoxins; antifungal properties; chromatography; cultivars; fungi; genes; peanuts; seeds; spectroscopy; trypsin;     Show all 12 Subjects
Abstract:
... Significant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have identified and characterized, at biochemical and molecular levels, plant protease inhibitors (PPIs) produced in peanut seeds of the resistant PI 337394 and the susceptible Forman ...
DOI:
10.1094/PHYTO-09-16-0346-R

14. Physicochemical characterization of jicaro seeds (Crescentia alata H.B.K.): A novel protein and oleaginous seed

Author:
Corrales, C.V.; Fliedel, G.; Perez, A.M.; Servent, A.; Prades, A.; Dornier, M.; Lomonte, B.; Vaillant, F.
Source:
Subtropical plant science 2017 v.56 pp. 84-92
ISSN:
0889-1575
Subject:
trypsin inhibitors,   etc   ; Crescentia alata; albumins; cotyledons; dietary fiber; digestibility; drought; essential amino acids; fruits; molecular weight; oleic acid; phytic acid; polyacrylamide gel electrophoresis; polyphenols; proteomics; seeds; soybeans; sugars; trees; trypsin; Central America;     Show all 21 Subjects
Abstract:
... Jicaro (Crescentia alata) is a tropical tree native to Mesoamerica well adapted to severe drought conditions. The seeds of the fruits were analyzed for protein, fatty acid, dietary fiber, phytate, polyphenol, tannin, tocopherol, sugar, mineral, amino acid and trypsin inhibitor contents. The jicaro cotyledons contained 43.6±1.15g protein/100g and 38.0±0.20g fat/100g (d.w.), which is comparable to m ...
DOI:
10.1016/j.jfca.2016.11.007

15. Trypsin and trypsin inhibitor bind milk beta-lactoglobulin: Protein–protein interactions and morphology

Author:
Chanphai, P.; Tajmir-Riahi, H.A.
Source:
International journal of biological macromolecules 2017 v.96 pp. 754-758
ISSN:
0141-8130
Subject:
trypsin inhibitors,   etc   ; aqueous solutions; beta-lactoglobulin; binding capacity; hydrogen bonding; milk; pH; protein-protein interactions; spectroscopy; thermodynamics; transmission electron microscopy; trypsin; van der Waals forces;     Show all 13 Subjects
Abstract:
... Conjugation of trypsin and trypsin inhibitor with milk beta-lactoglobulin (b-LG) was studied in aqueous solution at physiological pH. Multiple spectroscopic methods and transmission electron microscopy (TEM) were used to characterize protein–protein interactions and protein morphology. Thermodynamic analysis ΔH (−24 to −11kJMol−1), ΔS (−30 to −5JMol−1K−1) and ΔG (−12 to −10kJMol−1) showed that pro ...
DOI:
10.1016/j.ijbiomac.2016.12.075

16. Characterization of Endogenous Protease and the Changes in Proteolytic Activity of Acetes vulgaris and Macrobrachium lanchesteri During Kapi Production

Author:
Pongsetkul, Jaksuma; Benjakul, Soottawat; Sumpavapol, Punnanee; Osako, Kazufumi; Faithong, Nandhsha
Source:
Journal of food biochemistry 2017 v.41 no.1
ISSN:
0145-8884
Subject:
trypsin inhibitors,   etc   ; Acetes; Macrobrachium; actin; bacteria; chymotrypsin; condiments; fermentation; fluorides; hydrolysis; myosin heavy chains; oligopeptides; pH; palatability; proteolysis; raw materials; salting; shrimp; sodium chloride; soybeans; trypsin;     Show all 21 Subjects
Abstract:
... Characteristics of endogenous proteases of shrimp, Acetes vulgaris (AP) and Macrobrachium lanchesteri (MP) as well as the changes in proteolytic activity during Kapi production were investigated. Maximal activity of AP and MP was found at pH 7, 60C and pH 8, 60C, respectively. Activity of both proteases decreased with increasing NaCl concentration (0–30%). Both extracts were strongly inhibited by ...
DOI:
10.1111/jfbc.12311

17. Fall Armyworm-Associated Gut Bacteria Modulate Plant Defense Responses

Author:
Acevedo, Flor E.; Peiffer, Michelle; Tan, Ching-Wen; Stanley, Bruce A.; Stanley, Anne; Wang, Jie; Jones, Asher G.; Hoover, Kelli; Rosa, Cristina; Luthe, Dawn; Felton, Gary
Source:
Molecular plant-microbe interactions 2017 v.30 no.2 pp. 127-137
ISSN:
0894-0282
Subject:
trypsin inhibitors,   etc   ; Klebsiella; Pantoea ananatis; Raoultella; Spodoptera frugiperda; bacteria; catechol oxidase; corn; gene expression regulation; genes; herbivores; host plants; insect larvae; insects; intestinal microorganisms; mechanical damage; peroxidase; proteins; saliva; tomatoes; trypsin;     Show all 21 Subjects
Abstract:
... Mechanical damage caused by insect feeding along with components present in insect saliva and oral secretions are known to induce jasmonic acid–mediated defense responses in plants. This study investigated the effects of bacteria from oral secretions of the fall armyworm Spodoptera frugiperda on herbivore-induced defenses in tomato and maize plants. Using culture-dependent methods, we identified s ...
DOI:
10.1094/MPMI-11-16-0240-R

18. Psc-AFP from Psoralea corylifolia L. overexpressed in Pichia pastoris increases antimicrobial activity and enhances disease resistance of transgenic tobacco

Author:
Luo, Xiu-Mei; Xie, Cheng-Jian; Wang, De; Wei, Yun-Min; Cai, Jie; Cheng, Shan-Shan; Yang, Xing -Yong; Sui, An -Ping
Source:
Applied microbiology and biotechnology 2017 v.101 no.3 pp. 1073-1084
ISSN:
0175-7598
Subject:
trypsin inhibitors,   etc   ; Alternaria alternata; Alternaria brassicae; Aspergillus niger; Cullen corylifolium; Pichia pastoris; Ralstonia solanacearum; Thanatephorus cucumeris; amino acids; antimicrobial properties; antimicrobial proteins; bioassays; complementary DNA; conidia; disease resistance; gene overexpression; genes; germination; molecular cloning; mycelium; plant growth; rapid amplification of cDNA ends; seeds; signal peptide; tobacco; transgenic plants; trypsin; yeasts;     Show all 28 Subjects
Abstract:
... Psc-AFP, isolated from the seeds of Psoralea corylifolia L., is an antimicrobial protein with trypsin inhibitor activity. Its encoding gene was cloned by 3′- rapid amplification of cDNA ends (RACE) combined with Y-shaped adaptor-dependent extension (YADE) method. The gene Psc-AFP encodes a protein of 203 amino acids with a deduced signal peptide of 24 residues. The growth inhibition effect exerted ...
DOI:
10.1007/s00253-016-7768-1

19. Antioxidative Activitiy of Protein Hydrolysate from the Muscle of Common Kilka (Clupeonella cultriventris caspia) Prepared Using the Purified Trypsin from Common Kilka Intestine

Author:
Zamani, Abbas; Madani, Rasool; Rezaei, Masoud; Benjakul, Soottawat
Source:
Journal of aquatic food product technology 2017 v.26 no.1 pp. 2-16
ISSN:
1547-0636
Subject:
trypsin inhibitors,   etc   ; 2,2-diphenyl-1-picrylhydrazyl; Clupeonella cultriventris; ammonium sulfate; antioxidant activity; chromatography; fish protein hydrolysate; hydrolysates; hydrolysis; intestines; molecular weight; muscles; pH; polyacrylamide gel electrophoresis; sodium dodecyl sulfate; soybeans; trypsin;     Show all 17 Subjects
Abstract:
... Trypsin from the intestine of common kilka (Clupeonella cultriventris caspia) was purified using ammonium sulfate precipitation (30–50% saturation), Sephadex G-75, and DEAE-cellulose chromatography with the purity of 30-fold and the yield of 12%. The molecular weight of trypsin was estimated to be 23.2 kDa based on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The trypsin ...
DOI:
10.1080/10498850.2015.1073822

20. Continuous method to determine the trypsin inhibitor activity in soybean flour

Author:
Coscueta, Ezequiel R.; Pintado, Manuela E.; Picó, Guillermo A.; Knobel, Gastón; Boschetti, Carlos E.; Malpiedi, Luciana Pellegrini; Nerli, Bibiana B.
Source:
Food chemistry 2017 v.214 pp. 156-161
ISSN:
0308-8146
Subject:
trypsin inhibitors,   etc   ; acetic acid; analytical methods; centrifugation; filtration; nutritive value; soy flour; trypsin; turbidity;     Show all 9 Subjects
Abstract:
... The determination of trypsin inhibitor (TI) activity is of importance to evaluate the nutritional value of soybean flours. An analytical method, which involves a continuous spectrophotometric rate determination for trypsin activity against the substrate N-benzoyl-DL-arginine p-nitroanilide, is proposed as an alternative to the standard discontinuous assay. Stopping the reaction with acetic acid an ...
DOI:
10.1016/j.foodchem.2016.07.056