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- Khan, Sharik R.; Kuzminov, Andrei
- Analytical biochemistry 2017 v.526 pp. 66-68
- circular DNA, etc ; DNA repair; Escherichia coli; chromosomes; pulsed-field gel electrophoresis; ultraviolet radiation; wells; Show all 7 Subjects
- ... We showed before that long linear DNA molecules containing single-strand interruptions and undergoing pulsed-field gel electrophoresis (PFGE) tend to break into subfragments (electrophoretic nick instability). Here we show that circular chromosomal DNA with single-strand interruptions remains in the wells during PFGE. This means that the presence of nicks in immobile circular DNA is not enough to ...
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