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- van der Kamp, Marc W., et al. Show all 4 Authors
- Biochemistry 2017 v.56 no.48 pp. 6377-6388
- Gibbs free energy; amide hydrolases; biosensors; creatine; creatinine; energy; enzymatic reactions; hydrogen bonding; metal ions; mutants; mutation; nitrogen; quantum mechanics; renal function
- ... Creatininase catalyzes the conversion of creatinine (a biosensor for kidney function) to creatine via a two-step mechanism: water addition followed by ring opening. Water addition is common to other known cyclic amidohydrolases, but the precise mechanism for ring opening is still under debate. The proton donor in this step is either His178 or a water molecule bound to one of the metal ions, and th ...