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1. ALIGNSEC: viewing protein secondary structure predictions within large multiple sequence alignments

Author:
Deléage, Gilbert
Source:
Bioinformatics 2017 v.33 no.24 pp. 3991-3992
ISSN:
1460-2059
Subject:
protein secondary structure,   etc   ; bioinformatics; prediction; sequence alignment;     Show all 4 Subjects
Abstract:
... ALIGNSEC is a module within ANTHEPROT designed for the interactive display, edition and printing of large-scale multiple alignments integrating secondary structure predictions. The ALIGNSEC module is part of the ANTHEPROT package (http://antheprot-pbil.ibcp.fr) which can be used freely for academic users. It is running on Windows Operating systems. For commercial use, please contact the author. gi ...
DOI:
10.1093/bioinformatics/btx521

2. Thermostability enhancement of chitosanase CsnA by fusion a family 5 carbohydrate-binding module

Author:
Han, Yujuan; Gao, Peixin; Yu, Wengong; Lu, Xinzhi
Source:
Biotechnology letters 2017 v.39 no.12 pp. 1895-1901
ISSN:
0141-5492
Subject:
protein secondary structure,   etc   ; carbohydrate binding; catalytic activity; chitosanase; thermal stability;     Show all 5 Subjects
Abstract:
... OBJECTIVE: To determine the effects of carbohydrate-binding modules (CBMs) on the thermostability and catalytic efficiency of chitosanase CsnA. RESULTS: Three CBMs (BgCBM5, PfCBM32-2 and AoCBM35) were engineered at the C-terminus of chitosanase CsnA to create hybrid enzymes CsnA-CBM5, CsnA-CBM32 and CsnA-CBM35. K ₘ values of all the hybrid enzymes were lower than that of the wild type (WT) enzyme; ...
DOI:
10.1007/s10529-017-2406-2

3. Fourier Analysis of Conservation Patterns in Protein Secondary Structure

Author:
Palaniappan, Ashok; Jakobsson, Eric
Source:
Computational and Structural Biotechnology Journal 2017 v.15 pp. 265-270
ISSN:
2001-0370
Subject:
protein secondary structure,   etc   ; asymmetry; hydrophobicity; potassium; potassium channels;     Show all 5 Subjects
Abstract:
... Residue conservation is a common observation in alignments of protein families, underscoring positions important in protein structure and function. Though many methods measure the level of conservation of particular residue positions, currently we do not have a way to study spatial oscillations occurring in protein conservation patterns. It is known that hydrophobicity shows spatial oscillations i ...
DOI:
10.1016/j.csbj.2017.02.002
PubMed:
28316759
PubMed Central:
PMC5342988

4. Fourier-based classification of protein secondary structures

Author:
Shu, Jian-Jun; Yong, Kian Yan
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
protein secondary structure,   etc   ; amino acids; genes; hydrophobicity; prediction;     Show all 5 Subjects
Abstract:
... The correct prediction of protein secondary structures is one of the key issues in predicting the correct protein folded shape, which is used for determining gene function. Existing methods make use of amino acids properties as indices to classify protein secondary structures, but are faced with a significant number of misclassifications. The paper presents a technique for the classification of pr ...
DOI:
10.1016/j.bbrc.2017.02.117

5. The secondary structure of proteins in liquid, frozen, and dried egg-white samples: Effect of gamma irradiation treatment

Author:
Uygun-Sarıbay, Mine; Ergun, Ece; Kalaycı, Yakup; Köseoğlu, Turhan
Source:
International journal of food properties 2017 v.20 no.sup2 pp. 1195-1203
ISSN:
1532-2386
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; egg albumen; gamma radiation; irradiation; liquids; reflectance; viscosity;     Show all 8 Subjects
Abstract:
... In this study, the effect of gamma irradiation treatment on the secondary structure of the whole egg-white proteins was investigated. Liquid, frozen, and dried egg-white samples were subjected to gamma irradiation doses of 0, 1, 2, and 3 kGy. Protein secondary structures were determined by Attenuated Total Reflectance Fourier-Transform Infrared (ATR-FTIR) spectroscopy. In liquid egg-white samples, ...
DOI:
10.1080/10942912.2016.1241263

6. An introduction to deep learning on biological sequence data: examples and solutions

Author:
Jurtz, Vanessa Isabell; Johansen, Alexander Rosenberg; Nielsen, Morten; Almagro Armenteros, Jose Juan; Nielsen, Henrik; Sønderby, Casper Kaae; Winther, Ole; Sønderby, Søren Kaae
Source:
Bioinformatics 2017 v.33 no.22 pp. 3685-3690
ISSN:
1460-2059
Subject:
protein secondary structure,   etc   ; algorithms; artificial intelligence; bioinformatics; data collection; major histocompatibility complex; neural networks; peptides; prediction;     Show all 9 Subjects
Abstract:
... Deep neural network architectures such as convolutional and long short-term memory networks have become increasingly popular as machine learning tools during the recent years. The availability of greater computational resources, more data, new algorithms for training deep models and easy to use libraries for implementation and training of neural networks are the drivers of this development. The us ...
DOI:
10.1093/bioinformatics/btx531

7. Measuring Intracellular Secondary Structure of a Cell-Penetrating Peptide in Situ

Author:
Fleissner, Frederik; Pütz, Sabine; Schwendy, Mischa; Bonn, Mischa; Parekh, Sapun H.
Source:
Analytical chemistry 2017 v.89 no.21 pp. 11310-11317
ISSN:
1520-6882
Subject:
protein secondary structure,   etc   ; cytosol; endosomes; isotope labeling; lipids; lysosomes; microscopy; models; peptides; plasma membrane;     Show all 10 Subjects
Abstract:
... Cell-penetrating peptides (CPPs) are short peptide sequences that can translocate across cellular plasma membranes and are thus potential delivery vectors for diagnostic and therapeutic applications. Many CPPs exhibit some sort of structural polymorphism, where the secondary structure of the peptide is altered strongly by its local environment, which is believed to facilitate membrane translocatio ...
DOI:
10.1021/acs.analchem.7b01895

8. Unraveling the meaning of chemical shifts in protein NMR Proteins and proteomics

Author:
Berjanskii, Mark V.; Wishart, David S.
Source:
Biochimica et biophysica acta 2017 v.1865 no.11 pp. 1564-1576
ISSN:
1570-9639
Subject:
protein secondary structure,   etc   ; nuclear magnetic resonance spectroscopy; proteins; proteomics; surface area;     Show all 5 Subjects
Abstract:
... Chemical shifts are among the most informative parameters in protein NMR. They provide wealth of information about protein secondary and tertiary structure, protein flexibility, and protein-ligand binding. In this report, we review the progress in interpreting and utilizing protein chemical shifts that has occurred over the past 25years, with a particular focus on the large body of work arising fr ...
DOI:
10.1016/j.bbapap.2017.07.005

9. Capturing non-local interactions by long short-term memory bidirectional recurrent neural networks for improving prediction of protein secondary structure, backbone angles, contact numbers and solvent accessibility

Author:
Heffernan, Rhys; Yang, Yuedong; Paliwal, Kuldip; Zhou, Yaoqi
Source:
Bioinformatics 2017 v.33 no.18 pp. 2842-2849
ISSN:
1460-2059
Subject:
protein secondary structure,   etc   ; amino acids; artificial intelligence; bioinformatics; neural networks; prediction; solvents; surface area;     Show all 8 Subjects
Abstract:
... Motivation: The accuracy of predicting protein local and global structural properties such as secondary structure and solvent accessible surface area has been stagnant for many years because of the challenge of accounting for non-local interactions between amino acid residues that are close in three-dimensional structural space but far from each other in their sequence positions. All existing mach ...
DOI:
10.1093/bioinformatics/btx218

10. Decisive Role of Hydrophobicity on the Effect of Alkylammonium Chlorides on Protein Stability: A Terahertz Spectroscopic Finding B

Author:
Das Mahanta, Debasish; Samanta, Nirnay; Mitra, Rajib Kumar
Source:
The Journal of physical chemistry 2017 v.121 no.33 pp. 7777-7785
ISSN:
1520-5207
Subject:
protein secondary structure,   etc   ; absorption; bovine serum albumin; carbon; chlorides; hydrogen bonding; hydrophobic bonding; hydrophobicity; models; spectroscopy;     Show all 10 Subjects
Abstract:
... Many biologically important processes involve a subtle interplay between Columbic and hydrophobic interactions among molecular groups with water. A comprehensive understanding of such processes, specially while occurring simultaneously in the same molecule is of practical importance. In this contribution, we report the ultrafast (subpicosecond to picosecond) collective hydrogen bond dynamics of wa ...
DOI:
10.1021/acs.jpcb.7b04088

11. Protein secondary structure prediction based on the fuzzy support vector machine with the hyperplane optimization

Author:
Xie, Shangxin; Li, Zhong; Hu, Hailong
Source:
Gene 2017
ISSN:
0378-1119
Subject:
protein secondary structure,   etc   ; bioinformatics; data collection; databases; prediction; support vector machines;     Show all 6 Subjects
Abstract:
... The prediction of the protein secondary structure is a crucial point in bioinformatics and related fields. In the last years, machine learning methods have become a valuable tool, achieving satisfactory results. However, the prediction accuracy needs to be further ameliorated. This paper proposes a new method based on an improved fuzzy support vector machine (FSVM) for the prediction of the second ...
DOI:
10.1016/j.gene.2017.11.005

12. Effect of an Imposed Contact on Secondary Structure in the Denatured State of Yeast Iso-1-cytochrome c

Author:
Danielson, Travis A.; Stine, Jessica M.; Dar, Tanveer A.; Briknarova, Klara; Bowler, Bruce E.
Source:
Biochemistry 2017 v.56 no.51 pp. 6662-6676
ISSN:
1520-4995
Subject:
protein secondary structure,   etc   ; carbon; cytochrome c; guanidines; models; mutation; nitrogen; nuclear magnetic resonance spectroscopy; pH; stable isotopes; yeasts;     Show all 11 Subjects
Abstract:
... There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stable His–heme loop in the denatured state, suggestive of loop-induced residual struc ...
DOI:
10.1021/acs.biochem.7b01002

13. Hemoglobin bioconjugates with surface-protected gold nanoparticles in aqueous media: The stability depends on solution pH and protein properties

Author:
del Caño, Rafael; Mateus, Lucia; Sánchez-Obrero, Guadalupe; Sevilla, José Manuel; Madueño, Rafael; Blázquez, Manuel; Pineda, Teresa
Source:
Journal of colloid and interface science 2017 v.505 pp. 1165-1171
ISSN:
0021-9797
Subject:
protein secondary structure,   etc   ; anions; citrates; hemoglobin; isoelectric point; light scattering; nanogold; pH; protein corona; reflectance; ultraviolet-visible spectroscopy; zeta potential;     Show all 12 Subjects
Abstract:
... The identification of the factors that dictate the formation and physicochemical properties of protein-nanomaterial bioconjugates are important to understand their behavior in biological systems. The present work deals with the formation and characterization of bioconjugates made of the protein hemoglobin (Hb) and gold nanoparticles (AuNP) capped with three different molecular layers (citrate anio ...
DOI:
10.1016/j.jcis.2017.07.011

14. A new hybrid coding for protein secondary structure prediction based on primary structure similarity

Author:
Li, Zhong; Wang, Jing; Zhang, Shunpu; Zhang, Qifeng; Wu, Wuming
Source:
Gene 2017 v.618 pp. 8-13
ISSN:
0378-1119
Subject:
protein secondary structure,   etc   ; amino acid sequences; amino acids; hybrids; physicochemical properties; prediction; principal component analysis; support vector machines;     Show all 8 Subjects
Abstract:
... The coding pattern of protein can greatly affect the prediction accuracy of protein secondary structure. In this paper, a novel hybrid coding method based on the physicochemical properties of amino acids and tendency factors is proposed for the prediction of protein secondary structure. The principal component analysis (PCA) is first applied to the physicochemical properties of amino acids to cons ...
DOI:
10.1016/j.gene.2017.03.011

15. Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites

Author:
Fan, Mingcong; Hu, Ting; Zhao, Siming; Xiong, Shanbai; Xie, Jing; Huang, Qilin
Source:
Food chemistry 2017 v.218 pp. 221-230
ISSN:
0308-8146
Subject:
protein secondary structure,   etc   ; absorption; amylose; cassava starch; fish; gelling properties; gels; microstructure; protein concentrates; starch granules; storage modulus; synergism;     Show all 12 Subjects
Abstract:
... The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure. The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the pr ...
DOI:
10.1016/j.foodchem.2016.09.068

16. The effect of deep eutectic solvents on catalytic function and structure of bovine liver catalase

Author:
Harifi-Mood, Ali Reza; Ghobadi, Roohollah; Divsalar, Adeleh
Source:
International journal of biological macromolecules 2017 v.95 pp. 115-120
ISSN:
0141-8130
Subject:
protein secondary structure,   etc   ; aqueous solutions; binding capacity; catalase; cations; cattle; choline; circular dichroism spectroscopy; enzyme substrates; fluorescence; humans; liver; solvents;     Show all 13 Subjects
Abstract:
... Aqueous solutions of reline and glyceline, the most common deep eutectic solvents, were used as a medium for Catalase reaction. By some spectroscopic methods such as UV–vis, fluorescence and circular dichroism (CD) function and structure of Catalase were investigated in aqueous solutions of reline and glyceline. These studies showed that the binding affinity of the substrate to the enzyme increase ...
DOI:
10.1016/j.ijbiomac.2016.11.043

17. Effects of Ca2+ ions on bestrophin-1 surface films B Biointerfaces

Author:
Mladenova, Kirilka; Petrova, Svetla D.; Andreeva, Tonya D.; Moskova-Doumanova, Veselina; Topouzova-Hristova, Tanya; Kalvachev, Yuri; Balashev, Konstantin; Bhattacharya, Shomi S.; Chakarova, Christina; Lalchev, Zdravko; Doumanov, Jordan A.
Source:
Colloids and surfaces 2017 v.149 pp. 226-232
ISSN:
0927-7765
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; atomic force microscopy; calcium; colloids; epithelium; eye diseases; humans; hysteresis; ions; mutation;     Show all 11 Subjects
Abstract:
... Human bestrophin-1 (hBest1) is a transmembrane calcium-activated chloride channel protein – member of the bestrophin family of anion channels, predominantly expressed in the membrane of retinal pigment epithelium (RPE) cells. Mutations in the protein cause ocular diseases, named Bestrophinopathies. Here, we present the first Fourier transform infrared (FTIR) study of the secondary structure elemen ...
DOI:
10.1016/j.colsurfb.2016.10.023

18. Effects of heating on the secondary structure of proteins in milk powders using mid-infrared spectroscopy

Author:
Ye, M.P.; Zhou, R.; Shi, Y.R.; Chen, H.C.; Du, Y.
Source:
Journal of dairy science 2017 v.100 no.1 pp. 89-95
ISSN:
0022-0302
Subject:
protein secondary structure,   etc   ; adults; children; dried milk; heat; infant formulas; lipids; nutrients; reflectance; spectroscopy; temperature; thermal stability; whole milk;     Show all 13 Subjects
Abstract:
... Milk powder is an important source of protein for adults and children. Protein is very sensitive to heat, which may influence people’s usage of nutrients in milk powder. In this study, we describe the temperature-induced secondary structure of protein in milk powders. In this study, whole milk powder containing 24% protein and infant formula containing 11% protein were heated from 25 to 100°C. Att ...
DOI:
10.3168/jds.2016-11443

19. Bovine Serum Albumin Adsorption on TiO2 Nanoparticle Surfaces: Effects of pH and Coadsorption of Phosphate on Protein–Surface Interactions and Protein Structure

Author:
Xu, Zhenzhu; Grassian, Vicki H.
Source:
The Journal of Physical Chemistry C 2017 v.121 no.39 pp. 21763-21771
ISSN:
1932-7455
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; adsorption; blood; bovine serum albumin; denaturation; lungs; models; nanoparticles; pH; phosphates; quantitative analysis; stomach; thermogravimetry; titanium dioxide;     Show all 15 Subjects
Abstract:
... Protein adsorption on nanoparticle surfaces plays a critical role in biological systems, and bovine serum albumin (BSA) is a useful model protein to study due to its high abundance and similar properties as its human variant. Herein, a quantitative understanding of the interaction between titanium dioxide (TiO₂) nanoparticle (22 nm average diameter) and BSA was carried out to explore the effect of ...
DOI:
10.1021/acs.jpcc.7b07525

20. Changes in physicochemical properties and protein structure of surimi enhanced with camellia tea oil

Author:
Zhou, Xuxia; Jiang, Shan; Zhao, Dandan; Zhang, Jianyou; Gu, Saiqi; Pan, Zhiyan; Ding, Yuting
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2017 v.84 pp. 562-571
ISSN:
0023-6438
Subject:
protein secondary structure,   etc   ; Raman spectroscopy; correlation; electron microscopy; gels; hardness; hydrophobic bonding; lipid content; oils; physicochemical properties; proteins; storage modulus; surimi; tea;     Show all 14 Subjects
Abstract:
... The objective of this study was to determine the effects of different concentrations of camellia tea oil on surimi gel physicochemical properties and protein secondary structure. With the increase of camellia tea oil concentration (0–8 g/100 g of surimi), surimi gel hardness, whiteness, WHC, overall acceptability, storage modulus (G′) and the indexes of ionic bonds and hydrophobic interactions wer ...
DOI:
10.1016/j.lwt.2017.03.026

21. Protein polymerization and water mobility in whole-wheat dough influenced by bran particle size distribution

Author:
Xiong, Licheng; Zhang, Binjia; Niu, Meng; Zhao, Siming
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2017 v.82 pp. 396-403
ISSN:
0023-6438
Subject:
protein secondary structure,   etc   ; absorption; arabinoxylan; bran; dough; gluten; nuclear magnetic resonance spectroscopy; particle size; particle size distribution; polymerization; whole wheat flour;     Show all 11 Subjects
Abstract:
... The influences of bran particle size distribution on the protein polymerization and water mobility in whole-wheat dough (WWD) were investigated. Four bran particle size distributions were obtained by fine-grinding treatments and the mean particle sizes of reconstituted whole-wheat flour (WWF) were 242, 160, 114, and 45 μm, respectively. Mixolab parameters showed that WWF water absorption was incre ...
DOI:
10.1016/j.lwt.2017.04.064

22. Characteristics of normal and waxy corn: physicochemical, protein secondary structure, dough rheology and chapatti making properties

Author:
Thakur, Sheetal; Singh, Narpinder; Kaur, Amritpal
Source:
Journal of food science and technology 2017 v.54 no.10 pp. 3285-3296
ISSN:
0022-1155
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; calcium; copper; corn meal; dough; dough development; iron; lipid content; magnesium; manganese; minerals; potassium; rheological properties; rheology; sodium; waxy corn; zinc;     Show all 18 Subjects
Abstract:
... The physicochemical, protein secondary structure, dough rheological and chapatti making properties of normal and waxy corn types were evaluated. Waxy corn grains showed the highest L* and b* value, while red-pigmented corn grains had the highest a* value. Higher accumulation of K, Mg, Na and Ca minerals in corn was recorded, while Cu, Mn, Fe and Zn were present in trace. Meal prepared from waxy co ...
DOI:
10.1007/s13197-017-2775-5
PubMed:
28974814
PubMed Central:
PMC5602993

23. Effect of salts and nonionic surfactants on thermal characteristics of egg white proteins

Author:
Nasabi, Mahshad; Labbafi, Mohsen; Mousavi, Mohammad E.; Madadlou, Ashkan
Source:
International journal of biological macromolecules 2017 v.102 pp. 970-976
ISSN:
0141-8130
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; additives; ammonium sulfate; coagulation; egg albumen; glycerol; heat; heat treatment; industry; nonionic surfactants; sodium chloride; sodium sulfate; solubility; thermal stability; turbidity;     Show all 16 Subjects
Abstract:
... Effect of salts (Sodium chloride (NaCl), Sodium sulfate (Na2SO4), Ammonium sulfate ((NH4)2SO4), and nonionic surfactants (glycerol, tween20, tween80) on thermal properties of egg white proteins as a whole were investigated. Egg white solutions with additive (0, 0.5 and 1%) were collected after 0, 1 and 2min heat treatment. Physico-chemical properties of egg white proteins were evaluated by measuri ...
DOI:
10.1016/j.ijbiomac.2017.04.102

24. Molecular forces involved in heat-induced freshwater surimi gel: Effects of various bond disrupting agents on the gel properties and protein conformation changes

Author:
Yu, Nannan; Xu, Yanshun; Jiang, Qixing; Xia, Wenshui
Source:
Food hydrocolloids 2017 v.69 pp. 193-201
ISSN:
0268-005X
Subject:
protein secondary structure,   etc   ; disulfide bonds; freshwater; gel strength; gels; hardness; hydrocolloids; hydrogen bonding; hydrophobic bonding; sodium dodecyl sulfate; storage modulus; surimi; temperature; texture; urea;     Show all 15 Subjects
Abstract:
... Urea, sodium dodecyl sulfate (SDS) and N-ethylmaleimide (NEM) were employed to monitor the contributions of hydrogen bonds, hydrophobic interactions and disulfide bonds in heat-induced surimi gel. Surimi gels were prepared with the bond disrupting agents followed by texture profile, gel strength, rheological and Raman analysis. The rheological analysis demonstrated that remarkable changes of stora ...
DOI:
10.1016/j.foodhyd.2017.02.003

25. Structural changes evaluation with Raman spectroscopy in meat batters prepared by different processes

Author:
Kang, Zhuang-Li; Li, Xiang; He, Hong-ju; Ma, Han-jun; Song, Zhao-jun
Source:
Journal of food science and technology 2017 v.54 no.9 pp. 2852-2860
ISSN:
0022-1155
Subject:
protein secondary structure,   etc   ; Raman spectroscopy; batters; chopping; disulfide bonds; hydrophobic bonding; light scattering; particle size; pork; scanning electron microscopy; spectrometers; thermal stability; tyrosine;     Show all 13 Subjects
Abstract:
... A comprehensive study was conducted to evaluate the structural changes of meat and protein of pork batters produced by chopping or beating process through the phase-contrast micrograph, laser light scattering analyzer, scanning electronic microscopy and Raman spectrometer. The results showed that the shattered myofibrilla fragments were shorter and particle-sizes were smaller in the raw batter pro ...
DOI:
10.1007/s13197-017-2723-4
PubMed:
28928525
PubMed Central:
PMC5583115

26. Chemotyping using synchrotron mid-infrared and X-ray spectroscopy to improve agricultural production1

Author:
Tanino, K.; Willick, I.R.; Hamilton, K.; Vijayan, P.; Jiang, Y.; Brar, G.S.; Yu, P.; Kalcsits, L.; Lahlali, R.; Smith, B.; Fowler, D. Brian; Kutcher, R.; Bueckert, R.; Warkentin, T.; Karunakaran, C.
Source:
Canadian journal of plant science 2017 v.97 no.6 pp. 982-996
ISSN:
1918-1833
Subject:
protein secondary structure,   etc   ; X-radiation; absorption; cell wall components; epicuticular wax; fluorescence; fruit crops; leaves; lipids; nondestructive methods; nutrients; pollen; spectroscopy; Saskatchewan;     Show all 14 Subjects
Abstract:
... Synchrotron techniques are powerful tools in material and environmental sciences; however, they are currently underutilized in plant research. The Canadian Light Source synchrotron at the University of Saskatchewan campus is the only such facility in Canada open to academic, government, and industrial clients. This review introduces the potential of synchrotron-based spectroscopic methods and its ...
DOI:
10.1139/cjps-2016-0376

27. Binding of ciprofloxacin to bovine serum albumin: Photophysical and thermodynamic aspects

Author:
Paul, Bijan K.; Guchhait, Nikhil; Bhattacharya, Subhash Chandra
Source:
Journal of photochemistry and photobiology 2017 v.172 pp. 11-19
ISSN:
1011-1344
Subject:
protein secondary structure,   etc   ; bovine serum albumin; calorimetry; ciprofloxacin; circular dichroism spectroscopy; drug therapy; drugs; energy; fluorescence; models; spectral analysis; stoichiometry; thermodynamics; titration; transport proteins;     Show all 15 Subjects
Abstract:
... The present work reveals the study of interaction of a promising chemotherapeutic drug ciprofloxacin (CFX) with a model transport protein bovine serum albumin (BSA). The occurrence of the drug-protein interaction is found to result in significant modulations of the fluorescence excitation and emission spectroscopic properties of CFX following interaction with BSA. However, the major focus of the s ...
DOI:
10.1016/j.jphotobiol.2017.04.026

28. Solvent-dependent binding interactions of the organophosphate pesticide, chlorpyrifos (CPF), and its metabolite, 3,5,6-trichloro-2-pyridinol (TCPy), with Bovine Serum Albumin (BSA): A comparative fluorescence quenching analysis

Author:
Dahiya, Vandana; Chaubey, Bhawna; Dhaharwal, Ashok K.; Pal, Samanwita
Source:
Pesticide biochemistry and physiology 2017 v.139 pp. 92-100
ISSN:
0048-3575
Subject:
protein secondary structure,   etc   ; bovine serum albumin; chlorpyrifos; circular dichroism spectroscopy; fluorescence; fluorescence emission spectroscopy; humans; metabolites; methanol; solvents; ultraviolet-visible spectroscopy;     Show all 11 Subjects
Abstract:
... Analysis of the interaction of pesticides and their metabolites with the cellular proteins has drawn considerable attention in past several years to understand the effect of pesticides on environment and mankind. In this study, we have investigated the binding interaction of Bovine Serum Albumin (BSA) with a widely used organophosphorous insecticide chlorpyrifos (CPF), and its stable metabolite, 3 ...
DOI:
10.1016/j.pestbp.2017.04.011

29. Analysis of European Honeybee (Apis Mellifera) Wings Using ATR-FTIR and Raman Spectroscopy: A Pilot Study

Author:
Machovič, V.; Lapčák, L.; Havelcová, M.; Borecká, L.; Novotná, Mi.; Novotná, Ma.; Javůrková, I.; Langrová, I.; Hájková, Š.; Brožová, A.; Titěra, Dalibor
Source:
Scientia Agriculturae Bohemica 2017 v.48 no.1 pp. 22-29
ISSN:
1805-9430
Subject:
protein secondary structure,   etc   ; Apis mellifera; Fourier transform infrared spectroscopy; Raman spectroscopy; chitin; discriminant analysis; honey bees; lipids; molecular conformation; multivariate analysis; phenylalanine; proteins; tryptophan; tyrosine; vibration; wings;     Show all 16 Subjects
Abstract:
... The infrared (ATR-FTIR) and Raman spectroscopy was used for the structural characterization of honeybee wings. Protein, chitin, and lipid characteristic spectral features were detected using both methods. The protein secondary structure was predominantly composed of the β-sheet molecular conformation with β-turns or coil contributions. The vibration modes of the side-chain aromatic amino acid resi ...
DOI:
10.1515/sab-2017-0004

30. Comparative analysis of variation and selection in the HCV genome

Author:
Patiño-Galindo, Juan Ángel; González-Candelas, Fernando
Source:
Infection, genetics, and evolution 2017 v.49 pp. 104-110
ISSN:
1567-1348
Subject:
protein secondary structure,   etc   ; epitopes; at-risk population; evolution; Hepatitis C virus; antibodies; heterozygosity; regression analysis; viruses; genes; CD4-positive T-lymphocytes; genetic variation; RNA; CD8-positive T-lymphocytes;     Show all 14 Subjects
Abstract:
... Genotype 1 of the hepatitis C virus (HCV) is the most prevalent of the variants of this virus. Its two main subtypes, HCV-1a and HCV-1b, are associated to differences in epidemic features and risk groups, despite sharing similar features in most biological properties. We have analyzed the impact of positive selection on the evolution of these variants using complete genome coding regions, and comp ...
DOI:
10.1016/j.meegid.2017.01.010

31. Protein Structural Features in Winter Wheat: Benchmarking Diversity in Ontario Hard and Soft Winter Wheat

Author:
Cao, Wei; Falk, Duane; Bock, Jayne E.
Source:
Cereal chemistry 2017 v.94 no.2 pp. 199-206
ISSN:
0009-0352
Subject:
protein secondary structure,   etc   ; batters; breadmaking quality; dough; flour; gluten; models; polymers; principal component analysis; rheological properties; solvents; thiols; water content; wheat classes; winter wheat; Ontario;     Show all 16 Subjects
Abstract:
... A set of 32 winter wheat lines and varieties was selected to benchmark Ontario winter wheat as a first step toward improving quality. Protein secondary structure, total and accessible thiols, rheological properties, gluten aggregation kinetics, and network forming capabilities of different polymers were determined for each wheat line. Results revealed that there were statistically significant diff ...
DOI:
10.1094/CCHEM-03-16-0073-R

32. Characteristics of surface layer proteins from two new and native strains of Lactobacillus brevis

Author:
Mobarak Qamsari, Elahe; Kasra Kermanshahi, Rouha; Erfan, Mohammad; Ghadam, Parinaz; Sardari, Soroush; Eslami, Neda
Source:
International journal of biological macromolecules 2017 v.95 pp. 1004-1010
ISSN:
0141-8130
Subject:
protein secondary structure,   etc   ; Lactobacillus brevis; circular dichroism spectroscopy; differential scanning calorimetry; freeze drying; light scattering; lithium chloride; polyacrylamide gel electrophoresis; proteins; staining; thermal properties; zeta potential;     Show all 12 Subjects
Abstract:
... In this work, some important characteristics of surface layer (S-layer) proteins extracted from two new and native Lactobacillus strains, L.brevis KM3 and L.brevis KM7, were investigated. The presence of S-layer on the external surface of L.brevis KM3 was displayed by thin sectioning and negative staining. SDS-PAGE analysis were shown same dominant protein bands approximately around 48kDa for both ...
DOI:
10.1016/j.ijbiomac.2016.10.089

33. Whey protein gelation induced by enzymatic hydrolysis and heat treatment: Comparison of creep and recovery behavior

Author:
Spotti, Maria Julia; Tarhan, Özgür; Schaffter, Sam; Corvalan, Carlos; Campanella, Osvaldo H.
Source:
Food hydrocolloids 2017 v.63 pp. 696-704
ISSN:
0268-005X
Subject:
protein secondary structure,   etc   ; Bacillus licheniformis; denaturation; enzymatic hydrolysis; gelation; gels; heat treatment; hydrocolloids; hydrolysis; models; proteinases; storage modulus; temperature; whey protein;     Show all 14 Subjects
Abstract:
... Whey proteins can form different types of gels depending on the method used for their preparation. The purpose of this study was to characterize and compare gels obtained by heating and enzymatic (Bacillus Licheniformis protease) treatments in terms of gelation kinetic, gel mechanical and microstructural properties, and conformational changes of the protein secondary structure due to protein hydro ...
DOI:
10.1016/j.foodhyd.2016.10.014

34. Probing the binding mechanism of photoresponsive azobenzene polyamine derivatives with human serum albumin

Author:
Deiana, M.; Pokladek, Z.; Ziemianek, M.; Tarnowicz, N.; Mlynarz, P.; Samoc, M.; Matczyszyn, K.
Source:
RSC advances 2017 v.7 no.10 pp. 5912-5919
ISSN:
2046-2069
Subject:
protein secondary structure,   etc   ; DNA; active sites; binding capacity; bioactive properties; biochemical pathways; energy transfer; fluorescence; human serum albumin; melting; moieties; photosensitivity; polyamines; tryptophan;     Show all 14 Subjects
Abstract:
... Control over chemical and biochemical processes by agents sensitive either to internal or external stimuli has attracted much attention in recent years. In particular photosensitive polyamines have been recently used to photo-trigger the hybridization/melting of DNA as well as to modify its intrinsic morphology. These results prompted us to synthesize azobenzene-based polyamine derivatives and stu ...
DOI:
10.1039/c6ra26033k

35. 3D modelling of the pathogenic Leptospira protein LipL32: a bioinformatics approach

Author:
Kumaran, Sharmilah Kumari; Bakar, Mohd. Faizal Abu; Mohd-Padil, Hirzahida; Mat-Sharani, Shuhaila; Sakinah, S.; K, Poorani; Alsaeedy, Hiba; Peli, Amira; Wei, Teh Seoh; Ling, Mok Pooi; Hamat, Rukman Awang; Neela, Vasantha Kumari; Higuchi, Akon; Alarfaj, Abdullah A.; Rajan, Mariappan; Benelli, Giovanni; Arulselvan, Palanisamy; Kumar, S. Suresh
Source:
Acta tropica 2017
ISSN:
0001-706X
Subject:
protein secondary structure,   etc   ; Leptospira; bacterial antigens; bacterial proteins; bioinformatics; genes; germplasm conservation; hydrophilicity; hydrophobicity; leptospirosis; lipoproteins; models; outer membrane proteins; protein tertiary structure; signal peptide; virulent strains; zoonoses;     Show all 17 Subjects
Abstract:
... Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira species (Leptospiraceae). LipL32 is a highly abundant lipoprotein from the outer membrane proteins (OMPs) group, which highly conserved among pathogenic and intermediate Leptospira species. Several studies used LipL32 as a specific gene to identify the presence of leptospires. This research was aimed to study on the cha ...
DOI:
10.1016/j.actatropica.2017.09.011

36. Deodorization of Arthrospira platensis biomass for further scale‐up food applications

Author:
Cuellar‐Bermúdez, Sara P; Barba‐Davila, Bertha; Serna‐Saldivar, Sergio O; Parra‐Saldivar, Roberto; Rodriguez‐Rodriguez, José; Morales‐Davila, Sandra; Goiris, Koen; Muylaert, Koenraad; Chuck‐Hernández, Cristina
Source:
Journal of the science of food and agriculture 2017 v.97 no.15 pp. 5123-5130
ISSN:
0022-5142
Subject:
protein secondary structure,   etc   ; fatty acids; algae; Arthrospira platensis; odors; acetone; cakes; amino acids; smell; Fourier transform infrared spectroscopy; crude fat; hexane; gas chromatography-mass spectrometry; digestible protein; volatile compounds; protein sources; deodorization; biomass; ethanol; pigments; proteins; solvents;     Show all 22 Subjects
Abstract:
... BACKGROUND: Given the importance of A. platensis as a potential food protein source, we describe an affordable deodorization process that does not significantly affect the nutritional value of algae biomass. RESULTS: Ethanol, acetone or hexane were used to deodorize algae biomass and then to identify the profile of volatile compounds associated with its distinctive odor. Sensorial characteristics ...
DOI:
10.1002/jsfa.8391

37. Effect of gamma irradiation on structural, molecular, thermal and rheological properties of sunflower protein isolate

Author:
Malik, Mudasir Ahmad; Sharma, Harish Kumar; Saini, Charanjiv Singh
Source:
Food hydrocolloids 2017 v.72 pp. 312-322
ISSN:
0268-005X
Subject:
protein secondary structure,   etc   ; chemical elements; crosslinking; differential scanning calorimetry; dose response; fluorescence emission spectroscopy; gamma radiation; hydrocolloids; irradiation; loss modulus; molecular weight; protein isolates; protein tertiary structure; scanning electron microscopy; sunflower meal; sunflower seed protein; thermal stability; thermogravimetry;     Show all 18 Subjects
Abstract:
... Protein isolates were prepared from sunflower meal using alkaline extraction technique and were irradiated at different doses (0, 10, 20, 30, 40 and 50 kGy). Sunflower protein isolates were evaluated for structural, molecular, thermal and rheological properties. Secondary structure of proteins was disrupted with decrease in α-helix content and concomitant increase in β-sheet content. The change in ...
DOI:
10.1016/j.foodhyd.2017.06.011

38. Biochemical alterations in duckweed and algae induced by carrier solvents: Selection of an appropriate solvent in toxicity testing

Author:
Hu, Li‐Xin; Tian, Fei; Martin, Francis L.; Ying, Guang‐Guo
Source:
Environmental toxicology and chemistry 2017 v.36 no.10 pp. 2631-2639
ISSN:
0730-7268
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; Lemna minor; Selenastrum capricornutum; acetone; algae; carbohydrates; dimethyl sulfoxide; ethanol; growth retardation; hydrophobicity; lipids; methanol; multivariate analysis; protein phosphorylation; proteins; solvents; toxicity testing;     Show all 18 Subjects
Abstract:
... Carrier solvents are often used in aquatic toxicity testing for test chemicals with hydrophobic properties. However, the knowledge of solvent effects on test organisms remains limited. The present study aimed to determine the biochemical effects of the 4 common solvents methanol, ethanol, acetone, and dimethyl sulfoxide (DMSO) on 2 test species, Lemna minor and Raphidocelis subcapitata, by applyin ...
DOI:
10.1002/etc.3804

39. FTIR-spectroscopic and LA-ICP-MS imaging for combined hyperspectral image analysis of tumor models

Author:
Balbekova, A.; Bonta, M.; Török, S.; Ofner, J.; Döme, B.; Limbeck, A.; Lendl, B.
Source:
Analytical methods 2017 v.9 no.37 pp. 5464-5471
ISSN:
1759-9679
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; atomic absorption spectrometry; biomedical research; chemical species; elemental composition; histopathology; hyperspectral imagery; image analysis; models; multivariate analysis; neoplasms; platinum; therapeutics; trace elements; viability;     Show all 16 Subjects
Abstract:
... Modern chemical imaging techniques provide spatially resolved information on the molecular and elemental composition of samples with both high spatial and spectral resolution. Over the past few decades these techniques, in particular, Fourier transform infrared (FTIR) spectroscopy and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) have been successfully applied in histopat ...
DOI:
10.1039/c7ay01369h

40. Levels of Organochlorine Pesticides Are Associated with Amyloid Aggregation in Apex Avian Brains

Author:
Heys, Kelly A.; Shore, Richard F.; Pereira, M. Glória; Martin, Francis L.
Source:
Environmental science & technology 2017 v.51 no.15 pp. 8672-8681
ISSN:
1520-5851
Subject:
protein secondary structure,   etc   ; Accipiter; Alzheimer disease; Fourier transform infrared spectroscopy; Raman spectroscopy; amyloid; birds of prey; brain; environmental science; humans; indicator species; lipids; liver; organochlorine pesticides; risk; staining; wildlife;     Show all 17 Subjects
Abstract:
... Organochlorine (OC) pesticides pose a significant environmental risk to wildlife and humans and have been associated with Alzheimer’s disease (AD). This study aims to spectroscopically analyze brains from free-flying birds and link the results to OC exposure and consequent amyloid aggregation. As long-lived apex predators, predatory birds represent a sentinel species similar to humans. Therefore, ...
DOI:
10.1021/acs.est.7b00840

41. Binding interaction of sodium-N-dodecanoyl sarcosinate with hemoglobin and myoglobin: Physicochemical and spectroscopic studies with molecular docking analysis

Author:
Rudra, Suparna; Dasmandal, Somnath; Mahapatra, Ambikesh
Source:
Journal of colloid and interface science 2017 v.496 pp. 267-277
ISSN:
0021-9797
Subject:
protein secondary structure,   etc   ; calorimetry; circular dichroism spectroscopy; denaturation; density functional theory; enthalpy; fluorometry; heme proteins; hemoglobin; micelles; moieties; molecular models; myoglobin; pH; quantum mechanics; spectral analysis; surfactants; tensiometers; titration; tryptophan; ultraviolet-visible spectroscopy;     Show all 21 Subjects
Abstract:
... The interaction of an amino acid surfactant, sodium-N-dodecanoyl sarcosinate (SDDS), with two heme proteins, hemoglobin (Hb) and myoglobin (Mb), has been studied employing various physicochemical and spectroscopic techniques like tensiometry, UV–Vis spectroscopy, steady-state fluorometry, time-resolved fluorometry, circular dichroism (CD) spectroscopy, calorimetry and stopped flow kinetics at phys ...
DOI:
10.1016/j.jcis.2017.02.035

42. Spectroscopy study of the interaction between endocrine disruptor 4-OH-2,2′,3,4′-BDE and human serum albumin

Author:
Chen, Zhitao; Hu, Shuisheng; He, Xiaoyan; Guo, Longhua; Qiu, Bin; Lin, Zhenyu; Chen, Guonan; Cai, Zongwei
Source:
Analytical methods 2017 v.9 no.22 pp. 3338-3346
ISSN:
1759-9679
Subject:
protein secondary structure,   etc   ; binding sites; circular dichroism spectroscopy; dialysis; endocrine-disrupting chemicals; energy transfer; fluorescence emission spectroscopy; human serum albumin; hydrogen bonding; hydroxylation; polybrominated diphenyl ethers; thermodynamics; ultraviolet-visible spectroscopy; van der Waals forces;     Show all 14 Subjects
Abstract:
... Hydroxylated polybrominated diphenyl ethers (OH-PBDEs), as one of the derivatives of polybrominated diphenyl ethers (PBDEs), have received extensive concern recently. In this paper, the interaction of OH-PBDEs and human serum albumin (HSA) was investigated using 4-OH-2,2′,3,4′-tetrabromodiphenyl ether (4-OH-BDE-42) as a representative. The binding constant, the number of binding sites and the bind ...
DOI:
10.1039/c7ay00419b

43. Influence of extraction and solubilizing treatments on the molecular structure and functional properties of peanut protein

Author:
Ma, Tiezheng; Zhu, Hongguang; Wang, Jing; Wang, Qiang; Yu, Liangli (Lucy); Sun, Baoguo
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2017 v.79 pp. 197-204
ISSN:
0023-6438
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; emulsifying; enzymatic hydrolysis; foaming; food industry; functional properties; homogenization; manufacturing; microstructure; mixing; molecular weight; peanut protein; polyacrylamide gel electrophoresis; scanning electron microscopy; sodium dodecyl sulfate; solubility; solubilization; ultrasonic treatment; viscosity;     Show all 20 Subjects
Abstract:
... As primary functional property of protein in food industry, solubility was enhanced by various solubilization methods. Peanut protein was used to prepare product with high solubility by physical and enzymatic hydrolysis methods. Effects of extraction and solubilizing treatments on protein structure were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), scanning ele ...
DOI:
10.1016/j.lwt.2017.01.037

44. Molecular characterization and expression analysis of pearl millet plasma membrane proteolipid 3 (Pmp3) genes in response to abiotic stress conditions

Author:
Yeshvekar, Richa K.; Nitnavare, Rahul B.; Chakradhar, Thammineni; Bhatnagar-Mathur, Pooja; Reddy, Malireddy K.; Reddy, Palakolanu Sudhakar
Source:
Plant gene 2017 v.10 pp. 37-44
ISSN:
2352-4073
Subject:
protein secondary structure,   etc   ; Pennisetum glaucum; amino acids; gene expression; genes; heat stress; membrane potential; molecular cloning; nucleotide sequences; peptides; plasma membrane; prediction; proteins; salt stress; sequence analysis; stress response; stress tolerance;     Show all 17 Subjects
Abstract:
... Plasma membrane proteolipid 3 (Pmp3) is a gene family involved in abiotic stress response and cellular protection. Here we report cloning of two genes PgPmp3-1 and PgPmp3-2 from Pennisetum glaucum, and characterization with respect to their functions and responsiveness to various abiotic stresses. Both PgPmp3-1 and PgPmp3-2 genes are 171bp long and encode for 56 amino acid long peptides. PgPmp3 se ...
DOI:
10.1016/j.plgene.2017.05.002

45. FTIR analysis of molecular composition changes in hazel pollen from unpolluted and urbanized areas

Author:
Depciuch, J.; Kasprzyk, I.; Sadik, O.; Parlińska-Wojtan, M.
Source:
Aerobiologia 2017 v.33 no.1 pp. 1-12
ISSN:
0393-5965
Subject:
protein secondary structure,   etc   ; Corylus avellana; Fourier transform infrared spectroscopy; absorbance; air pollutants; air pollution; allergenicity; allergens; chemical composition; habitats; hypersensitivity; mutation; nucleic acids; people; pollen; proteins; scanning electron microscopy; urban areas; urbanization;     Show all 19 Subjects
Abstract:
... In this study, the effect of urbanization and environmental pollution on qualitative (structural) and quantitative changes of the Corylus avellana (hazel) pollen was investigated using scanning electron microscopy, Fourier Transform Infrared (FTIR) spectroscopy and curve-fitting analysis of amide I profile. The obtained spectroscopic results show significant variations in the fraction of proteins ...
DOI:
10.1007/s10453-016-9445-3
PubMed:
28255194
PubMed Central:
PMC5309270

46. The physicochemical properties of membranes correlate with the NADPH oxidase activity General subjects

Author:
Souabni, Hager; Wien, Frank; Bizouarn, Tania; Houée-Levin, Chantal; Réfrégiers, Matthieu; Baciou, Laura
Source:
Biochimica et biophysica acta 2017 v.1861 no.1 pp. 3520-3530
ISSN:
0304-4165
Subject:
protein secondary structure,   etc   ; NAD(P)H oxidase (H2O2-forming); NADP (coenzyme); activation energy; cell communication; circular dichroism spectroscopy; immune response; inflammation; lipid bilayers; microorganisms; models; phagocytes; physicochemical properties; sterols; structural proteins; temperature; toxicity; viscosity;     Show all 18 Subjects
Abstract:
... Phagocytes kill ingested microbes by exposure to high concentrations of toxic reactive species generated by NADPH-oxidases. This membrane-bound electron-transferring enzyme is tightly regulated by cellular signaling cascades. So far, molecular and biophysical studies of the NADPH-oxidase were performed over limited temperature ranges, which weaken our understanding of immune response or inflammato ...
DOI:
10.1016/j.bbagen.2016.06.028

47. Interaction of manganese nanoparticle with cytochrome c: A multi-spectroscopic study

Author:
Mansouri, Anali; Mousavi, Mina; Attar, Farnoosh; Saboury, Ali Akbar; Falahati, Mojtaba
Source:
International journal of biological macromolecules 2017
ISSN:
0141-8130
Subject:
protein secondary structure,   etc   ; Fourier transform infrared spectroscopy; binding sites; circular dichroism spectroscopy; cytochrome c; fluorescence; fluorescence emission spectroscopy; hydrophobicity; light scattering; manganese; nanoparticles; pH; phenylalanine; temperature; transmission electron microscopy; tryptophan; tyrosine; zeta potential;     Show all 18 Subjects
Abstract:
... In this paper, the conformational changes of cytochrome c (cyt c) upon interaction with manganese nanoparticle (Mn-NP) were examined using dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), zeta potential, fluorescence spectroscopy, and circular dichroism (CD) spectroscopy methods DLS and TEM analysis exhibited the structure of M ...
DOI:
10.1016/j.ijbiomac.2017.07.175

48. Determination of genetic effects of ATF3 and CDKN1A genes on milk yield and compositions in Chinese Holstein population

Author:
Han, Bo; Liang, Weijun; Liu, Lin; Li, Yanhua; Sun, Dongxiao
Source:
BMC genetics 2017 v.18 no.1 pp. 47
ISSN:
1471-2156
Subject:
protein secondary structure,   etc   ; linkage disequilibrium; dairy cattle; Holstein; 3' untranslated regions; cows; marker-assisted selection; lactating females; mammary glands; gene expression regulation; transcription factors; arginine; exons; milk yield; single nucleotide polymorphism; translation (genetics); milk composition; lactation; tryptophan; binding sites; genotype-phenotype correlation;     Show all 21 Subjects
Abstract:
... BACKGROUND: Our previous RNA-sequencing study revealed that the ATF3 and CDKN1A genes were remarkably differentially expressed between the mammary glands of lactating Holstein cows with extremely high and low milk protein and fat percentage so that both of them were considered as candidates for milk composition. Herein, we further verified whether these genes have genetic effects on milk productio ...
DOI:
10.1186/s12863-017-0516-4
PubMed:
28525989
PubMed Central:
PMC5437669

49. Recombinant Calponin of human filariid Brugia malayi: Secondary structure and immunoprophylactic potential

Author:
Verma, Shiv K.; Arora, Ashish; Murthy, P. Kalpana
Source:
Vaccine 2017 v.35 no.38 pp. 5201-5208
ISSN:
0264-410X
Subject:
protein secondary structure,   etc   ; Brugia malayi; Mastomys; Onchocerca volvulus; actin; adults; cell proliferation; digestive system diseases; filariasis; genes; humans; immunization; immunoglobulin G; inducible nitric oxide synthase; interferon-gamma; interleukin-1beta; interleukin-4; mast cells; microfilariae; molecular cloning; myosin; nitric oxide; parasites; tumor necrosis factor-alpha; vaccines; vision;     Show all 26 Subjects
Abstract:
... In the search for potential vaccine candidates for the control of human lymphatic filariasis, we recently identified calponin-like protein, that regulates actin/myosin interactions, in a proinflammatory fraction F8 (45.24–48.64kDa) of Brugia malayi adult worms. In the present study, the gene was cloned, expressed, and the recombinant Calponin of B. malayi (r-ClpBm) was prepared and characterized. ...
DOI:
10.1016/j.vaccine.2017.07.105