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You searched for: Author "Dai, Li-Shang" Remove constraint Author: "Dai, Li-Shang" Publication Year 2017 Remove constraint Publication Year: 2017
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1. Complete sequence and characterization of the Ectropis oblique mitochondrial genome and its phylogenetic implications

Author:
Dai, Li-Shang,   et al.   ; Kausar, Saima; Abbas, Muhammad Nadeem; Wang, Tian-Tian;     Show all 4 Authors
Source:
International journal of biological macromolecules 2017
ISSN:
0141-8130
Subject:
adenine; ribosomal RNA; thymine; mitochondrial genome; polymerase chain reaction; transfer RNA; cytosine; guanine; Ectropis obliqua; intergenic DNA; monophyly; start codon; statistical analysis; insects
Abstract:
... In the present study, we sequenced the entire mitochondrial genome (mitogenome) of Ectropis oblique using PCR amplification and sequencing methods The entire mitogenome is 15,356bp long, including 13 protein coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and A+T rich element. The base composition and gene arrangement are identical to those of other lepidopterans. Of the ...
DOI:
10.1016/j.ijbiomac.2017.09.093

2. Effects of Eicosanoid Biosynthesis Inhibitors on the Prophenoloxidase Transcript Level in BombyxmoriL

Author:
Dai, Li shang,   et al.   ; Zhang, Congfen; Zhu, Baojian; Liu, Chaoliang; Luo, Xuegang;     Show all 5 Authors
Source:
Journal of the Kansas Entomological Society 2017 v.90 no.3 pp. 194-203
ISSN:
0022-8567
Subject:
biosynthesis; defense mechanisms; eicosanoids; environmental factors; gene expression; hemocytes; innate immunity; lipopolysaccharides; prophenoloxidase; signal transduction; silkworms
Abstract:
... The silkworm, Bombyxmori L., has developed various defense mechanisms that reducethe negative effects of adverse environmental conditions. However, the mechanisms by which many physiological and ecological aspects work, including the functioning of the prophenoloxidase (pro-PO) system in Bombyxmori (BmPPO), are not completely understood. In our previous study, we found that eicosanoids mediated th ...
DOI:
10.2317/0022-8567-90.3.194

3. Suppressor of cytokine signaling 2-12 regulates antimicrobial peptides and ecdysteroid signaling pathways in Bombyx mori (Dazao)

Author:
Dai, Li-Shang,   et al.   ; Abbas, Muhammad Nadeem; Zhu, Bao-Jian; Kausar, Saima; Sun, Yu-Xuan; Tian, Ji Wu; Liu, Chao-Liang;     Show all 7 Authors
Source:
Journal of insect physiology 2017 v.103 pp. 47-56
ISSN:
0022-1910
Subject:
Bombyx mori; animals; antimicrobial peptides; cytokines; double-stranded RNA; ecdysterone; fat body; genes; hemocytes; insect physiology; larvae; nucleotide sequences; pathogens; quantitative polymerase chain reaction; signal transduction
Abstract:
... Suppressors of cytokine signaling (SOCS) are a potent negative regulator of diverse cytokine-related responses to maintain various physiological processes in animals. Here, we obtained the SOCS2-12 gene sequence of Bombyx mori (Dazao) (BmSOCS2-12) from the National Center for Biotechnology Information (NCBI) to study its expression profile in different tissues, as well as in the immune tissues fol ...
DOI:
10.1016/j.jinsphys.2017.10.004

4. A role of cathepsin L gene in innate immune response of crayfish (Procambarus clarkii)

Author:
Dai, Li-Shang,   et al.   ; Chu, Sheng-Hui; Yu, Xiao-Min; Li, Yan-Yan;     Show all 4 Authors
Source:
Fish & shellfish immunology 2017 v.71 pp. 246-254
ISSN:
1050-4648
Subject:
Escherichia coli; Procambarus clarkii; RNA interference; amino acids; cathepsin L; complementary DNA; crayfish; gene expression; genes; hepatopancreas; immune response; innate immunity; isoelectric point; lipopolysaccharides; messenger RNA; molecular weight; pathogens; polyclonal antibodies; polyinosinic-polycytidylic acid; quantitative polymerase chain reaction; rabbits; recombinant proteins; signal peptide; tissues
Abstract:
... Cathepsin L is one of the crucial enzyme superfamilies and involved in the immune responses. In the present study, cathepsin L gene from the red crayfish Procambarus clarkii, named PcCTSL, was cloned and characterized. The cDNA fragment of PcCTSL was 1026 bp in length, which encoded a putative protein of 341 amino acid residues with a molecular weight of 37.884 kDa. The theoretical isoelectric poi ...
DOI:
10.1016/j.fsi.2017.10.021

5. Molecular structure and functional characterization of the peroxiredoxin 5 in Procambarus clarkii following LPS and Poly I:C challenge

Author:
Dai, Li-Shang,   et al.   ; Wu, Liang; Zhou, Yang; Abbas, Muhammad Nadeem; Kausar, Saima; Chen, Qi; Jiang, Cheng-Xi;     Show all 7 Authors
Source:
Fish & shellfish immunology 2017 v.71 pp. 28-34
ISSN:
1050-4648
Subject:
DNA damage; Escherichia coli; Procambarus clarkii; amino acids; antibodies; antioxidants; chemical structure; complementary DNA; dose response; hemocytes; immune response; messenger RNA; muscles; oxidants; oxidative stress; pathogens; peroxiredoxin; polyinosinic-polycytidylic acid; recombinant proteins; stomach; tissues
Abstract:
... Peroxiredoxin (Prx) family members play a critical role in host defense against oxidative stress, and are also involved in immune responses following microbial infection. In the present study, we firstly cloned the cDNA of Peroxiredoxin 5 from Procambarus clarkii (denoted as PcPrx5) and investigated its immune functions towards LPS and Poly I:C exposure. The PcPrx5 cDNA was composed of 564 bp and ...
DOI:
10.1016/j.fsi.2017.09.072

6. Transcriptome analysis of hepatopancraes of Procambarus clarkii challenged with polyriboinosinic polyribocytidylic acid (poly I:C)

Author:
Dai, Li-Shang,   et al.   ; Abbas, Muhammad Nadeem; Kausar, Saima; Zhou, Yang;     Show all 4 Authors
Source:
Fish & shellfish immunology 2017 v.71 pp. 144-150
ISSN:
1050-4648
Subject:
Procambarus clarkii; biogenesis; gene expression regulation; hepatopancreas; high-throughput nucleotide sequencing; polyinosinic-polycytidylic acid; polymerase chain reaction; transcriptomics; unigenes
Abstract:
... Crustacean hepatopancreas regulates metabolic processes, biogenesis and innate immune processes, and the knowledge on its immune genes are crucial to understand antimicrobial mechanisms. In this study, we reported the transcriptomic profile of Procambarus clarkii hepatopancreas after poly I:C administration using high-throughput sequencing. Following de novo assembly 56,716 unigene sequences with ...
DOI:
10.1016/j.fsi.2017.10.010

7. Transcriptome profiling of red swamp crayfish (Procambarus clarkii) hepatopancreas in response to lipopolysaccharide (LPS) infection

Author:
Dai, Li-Shang,   et al.   ; Zhou, Miao; Abbas, Muhammad Nadeem; Kausar, Saima; Jiang, Cheng-Xi;     Show all 5 Authors
Source:
Fish & shellfish immunology 2017 v.71 pp. 423-433
ISSN:
1050-4648
Subject:
Procambarus clarkii; carcinogenesis; crayfish; gene ontology; hepatopancreas; immune response; lysosomes; peroxisome proliferator-activated receptors; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; transcriptome; transcriptomics; unigenes
Abstract:
... The RNA-sequencing followed by de novo assembly generated 61,912 unigene sequences of P. clarkii hepatopancreas. Comparison of gene expression between LPS challenged and PBS control samples revealed 2552 differentially expressed genes (DEGs). Of these sequences, 1162 DEGs were differentially up-regulated and 1360 DEGs differentially down-regulated. The DEGs were then annotated against gene ontolog ...
DOI:
10.1016/j.fsi.2017.10.030