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1. Establishing a Safe, Rapid, Convenient and Low-cost Antiviral Assay of Interferon Bioactivity Based on Recombinant VSV Expressing GFP

Author:
Chen, Weiye; Wen, Zhiyuan; Zhang, Jialin; Li, Cuicui; Huang, Kehe; Bu, Zhigao
Source:
Journal of virological methods 2017
ISSN:
0166-0934
Subject:
Vesiculovirus,   etc   ; antiviral properties; cell culture; fluorescence; green fluorescent protein; interferons; phenolsulfonphthalein;     Show all 7 Subjects
Abstract:
... The methods of the quantitative assay of the antiviral activity of interferons (IFNs) (type I, II or III) are very important during carrying out of the research of them, since they were found. Here a recombinant vesicular stomatitis virus expressing green fluorescent protein (GFP) (VSV/GFP) and MDBK cells were used to develop an antiviral assay (AVA) for IFNs. This method was carried out on a 96-w ...
DOI:
10.1016/j.jviromet.2017.08.007

2. The matrix protein of vesicular stomatitis virus inhibits host-directed transcription of target genes via interaction with the TFIIH subunit p8

Author:
Pan, Wei; Song, Deguang; He, Wenqi; Lu, Huijun; Lan, Yungang; Tong, Juanzhen; Gao, Feng; Zhao, Kui
Source:
Veterinary microbiology 2017 v.208 pp. 82-88
ISSN:
0378-1135
Subject:
Vesiculovirus,   etc   ; gene expression; innate immunity; mutagenesis; mutants; proteins; reporter genes; transcription (genetics); two hybrid system techniques; virus replication; viruses;     Show all 11 Subjects
Abstract:
... In response to viral infection, the host innate antiviral response is elicited to limit viral replication. Many viruses have evolved various strategies to circumvent the host antiviral response. It has been reported that matrix (M) protein of vesicular stomatitis virus (VSV) can inhibit host gene expression to evade the host innate immune response. However, the molecular mechanism remains unclear. ...
DOI:
10.1016/j.vetmic.2017.07.020

3. Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV

Author:
Huangfu, Chaoji; Ma, Yuyuan; Jia, Junting; Lv, Maomin; Zhu, Fengxuan; Ma, Xiaowei; Zhao, Xiong; Zhang, Jingang
Source:
Biologicals 2017 v.46 pp. 139-142
ISSN:
1045-1056
Subject:
Vesiculovirus,   etc   ; Suid alphaherpesvirus 1; Sindbis virus; glucose; sucrose; manufacturing; Encephalomyocarditis virus; pasteurization; viruses; Ungulate protoparvovirus 1; maltose; viral load; stabilizers;     Show all 13 Subjects
Abstract:
... Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than su ...
DOI:
10.1016/j.biologicals.2017.01.009

4. Incorporation of Viral Glycoprotein VSV-G Improves the Delivery of DNA by Erythrocyte Ghost into Cells Refractory to Conventional Transfection

Author:
Liu, Xin; Li, Yun-pan; Zhong, Zhen-min; Tan, Hui-qi; Lin, Hao-peng; Chen, Shao-jun; Fu, Yu-cai; Xu, Wen-can; Wei, Chi-ju
Source:
Applied biochemistry and biotechnology 2017 v.181 no.2 pp. 748-761
ISSN:
0273-2289
Subject:
Vesiculovirus,   etc   ; Western blotting; bone marrow; confocal microscopy; electrophoresis; endocytosis; erythrocytes; glycoproteins; luciferase; plasmids; stem cells; transfection; transgenes;     Show all 13 Subjects
Abstract:
... The objective of this study was to formulate a novel gene delivery system based on the erythrocyte ghost (EG) integrated with fusogenic viral glycoprotein vesicular stomatitis virus glycoprotein G (VSV-G). VSV-G proteins were harvested as condition medium of Ad293 cells carrying a VSV-G transgene and then incorporated into EG. Plasmid DNA was condensed by various transfection reagents. A luciferas ...
DOI:
10.1007/s12010-016-2246-x

5. Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection

Author:
Du, Taofeng; Shi, Yunpeng; Xiao, Shuqi; Li, Na; Zhao, Qin; Zhang, Angke; Nan, Yuchen; Mu, Yang; Sun, Yani; Wu, Chunyan; Zhang, Hongtao; Zhou, En-Min
Source:
BMC veterinary research 2017 v.13 no.1 pp. 298
ISSN:
1746-6148
Subject:
Vesiculovirus,   etc   ; Chikungunya virus; Hepatitis C virus; Porcine reproductive and respiratory syndrome virus; cell fusion; curcumin; drugs; genotype; macrophages; pandemic; proteins; swine; viruses;     Show all 13 Subjects
Abstract:
... BACKGROUND: Porcine reproductive and respiratory syndrome virus (PRRSV) could lead to pandemic diseases and huge financial losses to the swine industry worldwide. Curcumin, a natural compound, has been reported to serve as an entry inhibitor of hepatitis C virus, chikungunya virus and vesicular stomatitis virus. In this study, we investigated the potential effect of curcumin on early stages of PRR ...
DOI:
10.1186/s12917-017-1218-x
PubMed:
29017487
PubMed Central:
PMC5633875

6. EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein

Author:
Pan, Wei; Song, Deguang; He, Wenqi; Lu, Huijun; Lan, Yungang; Li, Hongli; Gao, Feng; Zhao, Kui
Source:
Veterinary microbiology 2017 v.212 pp. 59-66
ISSN:
0378-1135
Subject:
Vesiculovirus,   etc   ; RNA interference; amino acids; gene overexpression; genome; microbial growth; mutagenesis; mutants; signal transduction; two hybrid system techniques; virion; virus replication;     Show all 12 Subjects
Abstract:
... The matrix protein of vesicular stomatitis virus (VSV) performs multiple functions during viral genome replication and virion production and is involved in modulating multiple host signaling pathways that favor virus replication. To perform numerous functions within infected cells, the M protein needs to recruit cellular partners. To better understand the role of M during VSV replication, we looke ...
DOI:
10.1016/j.vetmic.2017.10.021

7. The effect of bovine BST2A1 on the release and cell-to-cell transmission of retroviruses

Author:
Liang, Zhibin; Zhang, Yang; Song, Jie; Zhang, Hui; Zhang, Suzhen; Li, Yue; Tan, Juan; Qiao, Wentao
Source:
Virology journal 2017 v.14 no.1 pp. 173
ISSN:
1743-422X
Subject:
Vesiculovirus,   etc   ; Bovine foamy virus; Bovine immunodeficiency virus; Bovine leukemia virus; Human immunodeficiency virus 1; antiviral properties; cattle; dimerization; glycosylation; humans; mutants; proteins; viruses;     Show all 13 Subjects
Abstract:
... BACKGROUND: Human BST2 (hBST2, also called Tetherin) is a host restriction factor that blocks the release of various enveloped viruses. BST2s from different mammals also possess antiviral activity. Bovine BST2s (bBST2s), bBST2A1 and bBST2A2, reduce production of cell-free bovine leukemia virus (BLV) and vesicular stomatitis virus (VSV). However, the effect of bBST2 on other retroviruses remains un ...
DOI:
10.1186/s12985-017-0835-0
PubMed:
28877726
PubMed Central:
PMC5588738

8. Ovarian Cancer Therapy by VSVMP Gene Mediated by a Paclitaxel-Enhanced Nanoparticle

Author:
Long, Jianlin; Yang, Yuping; Kang, Tianyi; Zhao, Wei; Cheng, Hao; Wu, Yujiao; Du, Ting; Liu, Beibei; Li, Yang; Luo, Feng; Gou, Maling
Source:
ACS applied materials & interfaces 2017 v.9 no.45 pp. 39152-39164
ISSN:
1944-8252
Subject:
Vesiculovirus,   etc   ; angiogenesis; antineoplastic activity; apoptosis; cell proliferation; encapsulation; gene therapy; genes; intraperitoneal injection; metastasis; nanoparticles; neoplasm cells; ovarian neoplasms; paclitaxel; polyethylene glycol; sulfates; transfection;     Show all 17 Subjects
Abstract:
... Nanoparticles have great promise for gene delivery. However, the transfection efficiency of nanoparticle-based gene delivery systems is always unsatisfied to meet the requirement of effective gene therapy. Herein, we used low-dosage paclitaxel to enhance a nanoscaled gene delivery system that was self-assembled from N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammoniummethyl sulfate and monomethox ...
DOI:
10.1021/acsami.7b10796

9. Clinical development of a recombinant Ebola vaccine in the midst of an unprecedented epidemic

Author:
Coller, Beth-Ann G.; Blue, Jeffrey; Das, Rituparna; Dubey, Sheri; Finelli, Lynn; Gupta, Swati; Helmond, Frans; Grant-Klein, Rebecca J.; Liu, Kenneth; Simon, Jakub; Troth, Sean; VanRheenen, Susan; Waterbury, Julie; Wivel, Ashley; Wolf, Jayanthi; Heppner, D. Gray; Kemp, Tracy; Nichols, Rick; Monath, Thomas P.
Source:
Vaccine 2017 v.35 no.35 pp. 4465-4469
ISSN:
0264-410X
Subject:
Vesiculovirus,   etc   ; World Health Organization; Zaire Ebola virus; adults; antibodies; clinical trials; enzyme-linked immunosorbent assay; genetics; glycoproteins; immune response; public health; seroconversion; vaccination; vaccines; Guinea; New Jersey;     Show all 16 Subjects
Abstract:
... The 2014–2016 Ebola outbreak caused over 28,000 cases and 11,000 deaths. Merck & Co. Inc., Kenilworth, NJ USA and NewLink Genetics are working with private and public partners to develop and license an Ebola vaccine that was evaluated extensively during the outbreak. The vaccine referred to as V920 is a recombinant vesicular stomatitis virus (rVSV) in which the VSV-G envelope glycoprotein (GP) is ...
DOI:
10.1016/j.vaccine.2017.05.097

10. Nanoparticles co-delivering pVSVMP and pIL12 for synergistic gene therapy of colon cancer

Author:
Xiao, Yuanyuan; Yang, Yuping; Wu, Yujiao; Wang, Chunmei; Cheng, Hao; Zhao, Wei; Li, Yang; Liu, Beibei; Long, Jianlin; Guo, Wenhao; Gao, Guangping; Gou, Maling
Source:
RSC advances 2017 v.7 no.52 pp. 32613-32623
ISSN:
2046-2069
Subject:
Vesiculovirus,   etc   ; adverse effects; antineoplastic activity; apoptosis; biodegradability; colorectal neoplasms; electrostatic interactions; gene therapy; genes; immune response; interleukin-12; intraperitoneal injection; metastasis; nanoparticles; neoplasm cells; plasmids;     Show all 16 Subjects
Abstract:
... Nanoparticles delivering therapeutic genes have promising applications in cancer treatments. Here, we used biodegradable heparin–polyethyleneimine (HPEI) nanoparticles to co-deliver two genes (vesicular stomatitis virus matrix protein, VSVMP; interleukin-12, IL12) for cancer therapy. These HPEI nanoparticles can bind the plasmid expressing VSVMP (pVSVMP) and the plasmid expressing IL12 (pIL12) thr ...
DOI:
10.1039/c7ra03727a

11. The localization of a heterologous displayed antigen in the baculovirus-budded virion determines the type and strength of induced adaptive immune response

Author:
Tavarone, Eugenia; Molina, Guido Nicolás; Amalfi, Sabrina; Peralta, Andrea; Molinari, Paula; Taboga, Oscar
Source:
Applied microbiology and biotechnology 2017 v.101 no.10 pp. 4175-4184
ISSN:
0175-7598
Subject:
Vesiculovirus,   etc   ; Baculoviridae; adjuvants; antigen presentation; antigens; capsid; coat proteins; cytotoxicity; glycoproteins; humoral immunity; immune response; mammals; models; ovalbumin; polypeptides; researchers; virion;     Show all 17 Subjects
Abstract:
... In the search of strategies of presentation of heterologous antigens to elicit humoral or cellular immune responses that modulate and properly potentiate each type of response, researchers have been studying baculovirus (BV) as vaccine vectors with promising results. For some years, several research groups explored different antigen presentation approaches using the BV AcNPV by expressing polypept ...
DOI:
10.1007/s00253-017-8183-y

12. Curcumin promotes the oncoltyic capacity of vesicular stomatitis virus for the treatment of prostate cancers

Author:
Fehl, Dylan J.; Ahmed, Maryam
Source:
Virus research 2017 v.228 pp. 14-23
ISSN:
0168-1702
Subject:
Vesiculovirus,   etc   ; animal models; antineoplastic activity; antineoplastic agents; apoptosis; cell culture; curcumin; mutants; neoplasm cells; phosphorylation; pro-apoptotic proteins; prostatic neoplasms; resveratrol; transcription factor NF-kappa B; virulence;     Show all 15 Subjects
Abstract:
... Vesicular stomatitis virus (VSV) matrix (M) protein mutants have been studied as oncolytic agents due to their capacity to effectively kill cancer cells while exhibiting low virulence in vivo. Despite encouraging results, many cancer cells maintain resistance to oncolytic VSV mutants in part due to residual antiviral responses. We sought to determine whether combination of VSV with natural agents ...
DOI:
10.1016/j.virusres.2016.10.020

13. Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections

Author:
Xu, Benhong; Gao, Yanpan; Zhan, Shaohua; Ge, Wei
Source:
Molecular Immunology 2017
ISSN:
0161-5890
Subject:
Vesiculovirus,   etc   ; proteins; proteome; protein synthesis; Human alphaherpesvirus 1; lysosomes; adaptive immunity; gene ontology; viruses; Listeria monocytogenes; proteomics; protein-protein interactions; pathogens;     Show all 13 Subjects
Abstract:
... Lysosomes play vital roles in both innate and adaptive immunity. It is widely accepted that lysosomes do not function exclusively as a digestive organelle. It is also involved in the process of immune cells against pathogens. However, the changes in the lysosomal proteome caused by infection with various microbes are still largely unknown, and our understanding of the proteome of the purified lyso ...
DOI:
10.1016/j.molimm.2017.04.002

14. Multiplex RT‐PCR and Automated Microarray for Detection of Eight Bovine Viruses

Author:
Lung, O.; Furukawa‐Stoffer, T.; Burton Hughes, K.; Pasick, J.; King, D. P.; Hodko, D.
Source:
Transboundary and emerging diseases 2017 v.64 no.6 pp. 1929-1934
ISSN:
1865-1674
Subject:
Vesiculovirus,   etc   ; Bovine alphaherpesvirus 1; detection limit; equipment; Bluetongue virus; Rinderpest morbillivirus; microarray technology; cattle; reverse transcriptase polymerase chain reaction; Bovine viral diarrhea virus 1; microbial detection; reverse transcription; viruses; Alcelaphine gammaherpesvirus 1; mixed infection; washing;     Show all 16 Subjects
Abstract:
... Microarrays can be a useful tool for pathogen detection as it allow for simultaneous interrogation of the presence of a large number of genetic sequences in a sample. However, conventional microarrays require extensive manual handling and multiple pieces of equipment for printing probes, hybridization, washing and signal detection. In this study, a reverse transcription (RT)–PCR with an accompanyi ...
DOI:
10.1111/tbed.12591

15. Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo

Author:
Weeratunga, Prasanna; Herath, ThilinaU. B.; Kim, Tae-Hwan; Lee, Hyun-Cheol; Kim, Jae-Hoon; Lee, Byeong-Hoon; Lee, Eun-Seo; Chathuranga, Kiramage; Chathuranga, W.A. Gayan; Yang, Chul-Su; Ma, JinYeul; Lee, Jong-Soo
Source:
The journal of microbiology 2017 v.55 no.11 pp. 909-917
ISSN:
1225-8873
Subject:
Vesiculovirus,   etc   ; Influenza A virus; Toxoplasma gondii; antiviral properties; cytokines; epithelium; herpes simplex; immunostimulants; innate immunity; interferons; intranasal administration; macrophages; mice; protective effect; secretion; toxoplasmosis; vaccine adjuvants; vaccines; virus replication; viruses;     Show all 20 Subjects
Abstract:
... Dense granule protein-7 (GRA-7) is an excretory protein of Toxoplasma gondii. It is a potential serodiagnostic marker and vaccine candidate for toxoplasmosis. Previous reports demonstrated that GRA-7 induces innate immune responses in macrophages by interacting with TRAF6 via the MyD88-dependent pathway. In the present study, we evaluated the antiviral activity and induction of an antiviral state ...
DOI:
10.1007/s12275-017-7392-5

16. Development of a micro-neutralization assay for ebolaviruses using a replication-competent vesicular stomatitis hybrid virus and a quantitative PCR readout

Author:
Lee, Stella S.; Phy, Kathryn; Peden, Keith; Sheng-Fowler, Li
Source:
Vaccine 2017 v.35 no.41 pp. 5481-5486
ISSN:
0264-410X
Subject:
Vesiculovirus,   etc   ; Ebolavirus; automation; bioterrorism; genes; hybrids; neutralization; neutralization tests; neutralizing antibodies; pathogenicity; public health; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; vaccine development; vesicular stomatitis; viral vaccines; viruses;     Show all 17 Subjects
Abstract:
... Development of vaccines against highly pathogenic viruses that could also be used as agents of bioterrorism is both a public health issue and a national security priority. Methods that can quantify neutralizing antibodies will likely be crucial in demonstrating vaccine effectiveness, as most licensed viral vaccines are effective due to their capacity to elicit neutralizing antibodies. Assays to de ...
DOI:
10.1016/j.vaccine.2017.03.019

17. Generation of recombinant bovine interferon tau in the human embryonic kidney cell line and its biological activity

Author:
Takahashi, Toru; Sakumoto, Ryosuke; Hayashi, Ken‐Go; Hosoe, Misa; Shirai, Junsuke; Hashizume, Kazuyoshi
Source:
Animal science journal = 2017 v.88 no.10 pp. 1498-1505
ISSN:
1344-3941
Subject:
Vesiculovirus,   etc   ; Western blotting; agarose; antibodies; antiviral properties; cattle; complementary DNA; gel chromatography; glycoproteins; hosts; human cell lines; humans; immunization; interferons; ion exchange; kidney cells; molecular weight; plasmid vectors; rabbits; recombinant proteins;     Show all 20 Subjects
Abstract:
... The objective of this study was to generate recombinant bovine interferon tau (rbIFNT) in mammalian hosts. The complementary DNA encoding bovine IFNT2 was cloned for the construction of pRcRSV‐bIFNT2 expression vector. The expression vector was transfected to 293 cells. Transfected cells harboring expression vector were selected with G418. Highly expressing clonal line was adapted to serum‐free su ...
DOI:
10.1111/asj.12820

18. The First Identification and Complete Genome of Senecavirus A Affecting Pig with Idiopathic Vesicular Disease in China

Author:
Wu, Q.; Zhao, X.; Bai, Y.; Sun, B.; Xie, Q.; Ma, J.
Source:
Transboundary and emerging diseases 2017 v.64 no.5 pp. 1633-1640
ISSN:
1865-1674
Subject:
Vesiculovirus,   etc   ; Enterovirus B; Foot-and-mouth disease virus; Senecavirus; Vesicular exanthema of swine virus; death; emerging diseases; farms; genome; mortality; piglets; reverse transcriptase polymerase chain reaction; sequence alignment; sows; swine vesicular disease; Brazil; China;     Show all 17 Subjects
Abstract:
... Senecavirus A (SVA) infection was recently confirmed in pigs in Brazil. In March, 2015, an outbreak of vesicular disease occurred in Guangdong, China, characterized by vesicular lesions in sows and acute death of neonatal piglets. Cumulative incidence of porcine idiopathic vesicular disease in farm A was 258, which had a total number of 5500 sows. Sows in farm B displayed typical vesicular symptom ...
DOI:
10.1111/tbed.12557

19. Quantitative profiling of innate immune activation by viral infection in single cells

Author:
TimmThese authors contributed equally to this work., Andrea C.; Warrick, Jay W.; Yin, John
Source:
Integrative biology 2017 v.9 no.9 pp. 782-791
ISSN:
1757-9708
Subject:
Vesiculovirus,   etc   ; cell death; cell viability; computer software; enzymes; gene expression; genome; green fluorescent protein; humans; image analysis; immune response; kinetics; prostatic neoplasms; reaction kinetics; red fluorescent protein; viral growth; virus replication; viruses;     Show all 18 Subjects
Abstract:
... Cells infected by viruses can exhibit diverse patterns of viral and cellular gene expression. The patterns arise in part from the stochastic or noisy reaction kinetics associated with the small number of genomes, enzymes, and other molecules that typically initiate virus replication and activate cellular anti-viral defenses. It is not known what features, if any, of the early viral or cellular gen ...
DOI:
10.1039/c7ib00082k

20. Pathogenicity of snakehead vesiculovirus in rice field eels (Monopterus albus)

Author:
Sun, Lindan; Tu, Jiagang; Yi, Lizhu; Chen, Wenjie; Zhao, Lijuan; Huang, Yunmao; Liang, Rishen; Li, Jun; Zhou, Meng; Lin, Li
Source:
Microbial pathogenesis 2017 v.110 pp. 578-585
ISSN:
0882-4010
Subject:
Vesiculovirus,   etc   ; Channidae; Monopterus albus; RNA; coculture; financial economics; fish; fish culture; genome; heart; hemorrhage; histopathology; immunohistochemistry; in situ hybridization; kidneys; liver; mortality; nucleotide sequences; pathogenicity; polymerization; reverse transcriptase polymerase chain reaction; reverse transcription; sequence analysis; tissues; vacuoles; viruses; China;     Show all 27 Subjects
Abstract:
... Snakehead vesiculovirus (SHVV) has caused mass mortality to cultured snakehead fish in China, resulting in enormous economic losses in snakehead fish culture. In this report, the whole genome of SHVV was sequenced. Interestingly, it shared more than 94% nucleotide sequence identity with Monopterus albus rhabdovirus (MoARV), which has caused great economic loss to cultured rice field eel (Monopteru ...
DOI:
10.1016/j.micpath.2017.07.042