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1. A genetically distinct Schistosoma from Radix luteola from Nepal related to Schistosoma turkestanicum: A phylogenetic study of schistosome and snail host

Author:
Devkota, Ramesh; Brant, Sara V.; Loker, Eric S.
Source:
Acta tropica 2016 v.164 pp. 45-53
ISSN:
0001-706X
Subject:
Schistosoma turkestanicum; cercariae; freshwater; genes; habitats; mammals; phylogeny; snails; surveys; India; Nepal
Abstract:
... During a survey of freshwater snails in the Terai region of southern Nepal, 16 of 2588 specimens of Radix luteola from 4 different habitats were found to be shedding schistosome cercariae. None of the 1411 specimens of Radix acuminata we collected were positive for schistosomes. Analysis of 28S, cox1, 16S and 12S sequences indicated that all the R. luteola-derived schistosomes were genetically ver ...
DOI:
10.1016/j.actatropica.2016.08.015

2. Association between fertilizer-mediated changes in microbial communities and Aedes albopictus growth and survival

Author:
Muturi, Ephantus J.; Ramirez, Jose L.; Rooney, Alejandro P.; Dunlap, Chris
Source:
Acta tropica 2016 v.164 pp. 54-63
ISSN:
0001-706X
Subject:
Aedes albopictus; ammonium sulfate; aquatic habitat; bacteria; bacterial communities; community structure; fertilizer application; fungal communities; fungi; genes; insect larvae; internal transcribed spacers; males; mineral fertilizers; nutrients; potassium chloride; potassium sulfate; ribosomal RNA; sex ratio; survival rate; transcription (genetics)
Abstract:
... Contamination of aquatic habitats with anthropogenic nutrients has been associated with an increase in mosquito larval populations but the underlying mechanisms remain poorly understood. We examined the individual and combined effects of two synthetic fertilizers (ammonium sulfate and potassium chloride) on Aedes albopictus survival, development time, and sex ratio. The bacterial and fungal commun ...

3. Biodegradation of pyrene by pseudomonas sp. JPN2 and its initial degrading mechanism study by combining the catabolic nahAc gene and structure-based analyses

Author:
Jin, Jingnan; Yao, Jun; Zhang, Qingye; Liu, Jianli
Source:
Chemosphere 2016 v.164 pp. 379-386
ISSN:
0045-6535
Subject:
DNA fragmentation; Pseudomonas; active sites; amino acids; bacteria; bioactive properties; biodegradation; bioremediation; enzymes; gas chromatography-mass spectrometry; genes; metabolites; molecular models; naphthalene; oil fields; petroleum; phthalates; polymerase chain reaction; China
Abstract:
... In this study, a pyrene-degrading bacterial strain Pseudomonas sp. JPN2 was isolated from crude oil in Dagang Oilfield, China. The degrading percent of the strain JPN2 to pyrene was increased with the extension of culture time and achieved a maximum of 82.88% after 25 d culture. Meanwhile, four metabolites 4,5-dihydroxy-4,5-dihydropyrene, 4-phenanthrol, 1-hydroxy-2-naphthoic acid and phthalate wer ...
DOI:
10.1016/j.chemosphere.2016.08.113

4. Dengue virus surveillance: Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil

Author:
Colombo, Tatiana Elias; Vedovello, Danila; Pacca-Mazaro, Carolina Colombelli; Mondini, Adriano; Araújo, João Pessoa; Cabrera, Eliana; Lopes, José Carlos; Penha dos Santos, Izalco Nuremberg; Negri Reis, Andréia Francesli; Costa, Fabiana Rodrigues; Antônio Cruz, Lilian Elisa Arão; Ferreira, Jurandir; de Oliveira Rocha, Eliseu Soares; Kroon, Erna Geessien; de Morais Bronzoni, Roberta Vieira; Vasilakis, Nikos; Nogueira, Maurício Lacerda
Source:
Acta tropica 2016 v.164 pp. 84-89
ISSN:
0001-706X
Subject:
Dengue virus; arboviruses; blood sampling; dengue; genes; genotype; mixed infection; monitoring; patients; phylogeny; reverse transcriptase polymerase chain reaction; serotypes; viral nonstructural proteins; Brazil
Abstract:
... Dengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in São José do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavi ...
DOI:
10.1016/j.actatropica.2016.09.004

5. Design, construction and validation of a Plasmodium vivax microarray for the transcriptome profiling of clinical isolates

Author:
Boopathi, Pon Arunachalam; Subudhi, Amit Kumar; Middha, Sheetal; Acharya, Jyoti; Mugasimangalam, Raja Chinnadurai; Kochar, Sanjay Kumar; Kochar, Dhanpat Kumar; Das, Ashis
Source:
Acta tropica 2016 v.164 pp. 438-447
ISSN:
0001-706X
Subject:
Plasmodium vivax; bioinformatics; genes; genomics; microarray technology; oligonucleotide probes; oligonucleotides; parasites; quantitative polymerase chain reaction; transcriptome; transcriptomics
Abstract:
... High density oligonucleotide microarrays have been used on Plasmodium vivax field isolates to estimate whole genome expression. However, no microarray platform has been experimentally optimized for studying the transcriptome of field isolates. In the present study, we adopted both bioinformatics and experimental testing approaches to select best optimized probes suitable for detecting parasite tra ...
DOI:
10.1016/j.actatropica.2016.10.002

6. Echinococcus granulosus sensu stricto, Echinococcus canadensis (G7), and Echinococcus ortleppi in fertile hydatid cysts isolated from cattle in Southern Brazil

Author:
Urach Monteiro, Danieli; de Azevedo, Maria Isabel; Weiblen, Carla; Correia Ribeiro, Tatiana; Emmanouilidis, Jéssica; Tonin, Alexandre Alberto; de Avila Botton, Sônia; de la Rue, Mário Luiz
Source:
Acta tropica 2016 v.164 pp. 41-44
ISSN:
0001-706X
Subject:
DNA; Echinococcus granulosus; animal organs; cattle; echinococcosis; genes; genotype; hydatids; intermediate hosts; microscopy; phylogeny; polymerase chain reaction; protoscoleces; sequence analysis; Brazil
Abstract:
... Echinococcosis is a cosmopolitan zoonotic infection that affects humans and animals. The aim of this study was to identify and characterize the fertile hydatid cysts from bovine viscera in order to verify different species and/or genotypes present in Southern Brazil. Firstly, cysts were collected from a slaughterhouse, which received animals from different regions of Rio Grande do Sul State (RS), ...
DOI:
10.1016/j.actatropica.2016.08.017

7. Effect of electrical stimulation on the fate of sulfamethoxazole and tetracycline with their corresponding resistance genes in three-dimensional biofilm-electrode reactors

Author:
Zhang, Shuai; Song, Hai-Liang; Yang, Xiao-Li; Yang, Ke-Yun; Wang, Xiao-Yang
Source:
Chemosphere 2016 v.164 pp. 113-119
ISSN:
0045-6535
Subject:
antibiotic resistance; carbon; chemical oxygen demand; electrical treatment; electrodes; genes; high-throughput nucleotide sequencing; microbial activity; microbial communities; risk; sludge; sulfamethoxazole; tetracycline; wastewater; wastewater treatment
Abstract:
... Three-dimensional biofilm-electrode reactors (3D-BERs), which possess a large effective area to drive the reductive degradation of contaminants, have recently attracted attention for wastewater treatment. There have been few studies of the potential and risks of the application of this system on the removal of antibiotics. Here four 3D-BERs were designed to initially assess the potential for elect ...
DOI:
10.1016/j.chemosphere.2016.08.076

8. Effects of β-diketone antibiotics on F1-zebrafish (Danio rerio) based on high throughput miRNA sequencing under exposure to parents

Author:
Zheng, Yuansi; Lin, Jiebo; Li, Jieyi; Zhang, Haifeng; Ai, Weiming; Wang, Xuedong; Dahlgren, Randy A.; Wang, Huili
Source:
Chemosphere 2016 v.164 pp. 41-51
ISSN:
0045-6535
Subject:
Danio rerio; antibiotics; bioinformatics; databases; egg yolk; gene expression regulation; genes; histopathology; in situ hybridization; microRNA; oocytes; parents; pollutants; progeny; quantitative polymerase chain reaction; reproduction; reverse transcriptase polymerase chain reaction; shrinkage; toxicity
Abstract:
... The toxicity of β-diketone antibiotics (DKAs), a class of ‘‘pseudo-persistent’’ environmental pollutants, to F0-zebrafish (Danio rerio) was investigated using 7-dpf F1-zebrafish miRNA sequencing and bioinformatics analyses. Based on relative expression, 47, 134 and 118 of 193 mature miRNAs were differentially expressed between control vs 6.25 mg/L, control vs 12.5 mg/L and 6.25 vs 12.5 mg/L treatm ...
DOI:
10.1016/j.chemosphere.2016.07.057

9. Efficacy of artemisinin-based combination therapies for the treatment of falciparum malaria in Pakistan (2007–2015): In vivo response and dhfr and dhps mutations

Author:
Kakar, Qutbuddin; Sheikh, Salma; Ahmed, Imran; Khan, Munir Ahmed; Jamil, Muhammad; ElMohammady, Hanan; Warsame, Marian
Source:
Acta tropica 2016 v.164 pp. 17-22
ISSN:
0001-706X
Subject:
Plasmodium falciparum; dihydrofolate reductase; genes; malaria; monitoring; mutants; mutation; patients; polymerase chain reaction; risk; Pakistan
Abstract:
... Artesunate+sulfadoxine-pyrimethamine (AS+SP) and artemether+lumefantrine (AL) are the first- and second line treatments, respectively, for the treatment of falciparum infections and dihydroartemsinin+piperaquine (DHA+PPQ) is a potential candidate in case AS+SP or AL fails in Pakistan. The therapeutic efficacies of AS+SP (5 sites in 2007, 2 sites in 2011 and 2 sites in 2012), AL (2 sites in 2012) a ...
DOI:
10.1016/j.actatropica.2016.08.006

10. Identification of antibiotic-resistant Escherichia coli isolated from a municipal wastewater treatment plant

Author:
Zanotto, Carlo; Bissa, Massimiliano; Illiano, Elena; Mezzanotte, Valeria; Marazzi, Francesca; Turolla, Andrea; Antonelli, Manuela; De Giuli Morghen, Carlo; Radaelli, Antonia
Source:
Chemosphere 2016 v.164 pp. 627-633
ISSN:
0045-6535
Subject:
Escherichia coli; Gram-negative bacteria; adenosine monophosphate; ampicillin; antibiotic resistance; beta-lactamase; biological treatment; chloramphenicol; coliform bacteria; disinfection; filtration; genes; irrigation; municipal wastewater; nucleotide sequences; peracetic acid; public health; sand; wastewater treatment; Italy
Abstract:
... The emergence and diffusion of antibiotic-resistant bacteria has been a major public health problem for many years now. In this study, antibiotic-resistance of coliforms and Escherichia coli were investigated after their isolation from samples collected in a municipal wastewater treatment plant in the Milan area (Italy) along different points of the treatment sequence: inflow to biological treatme ...
DOI:
10.1016/j.chemosphere.2016.08.040

11. Mitochondrial COI gene as a tool in the taxonomy of mosquitoes Culex subgenus Melanoconion

Author:
Torres-Gutierrez, Carolina; Bergo, Eduardo Sterlino; Emerson, Kevin J.; de Oliveira, Tatiane M.P.; Greni, Susan; Sallum, Maria Anice Mureb
Source:
Acta tropica 2016 v.164 pp. 137-149
ISSN:
0001-706X
Subject:
Culex; DNA barcoding; cytochrome-c oxidase; data collection; genes; genetic variation; insect taxonomy; insects; male genitalia; mitochondria; phylogeny; species diversity; tropics; Brazil
Abstract:
... The subgenus Melanoconion is the second largest subgenus within the genus Culex, with 160 described species. Several of the species are proven vectors of arboviruses, including West Nile virus, Venezuelan equine encephalitis virus complex and Eastern equine encephalomyelitis virus. Species of Melanoconion are well distributed from southern North America to most countries of South America and displ ...
DOI:
10.1016/j.actatropica.2016.09.007

12. Molecular surveillance of Plasmodium falciparum drug resistance markers reveals partial recovery of chloroquine susceptibility but sustained sulfadoxine-pyrimethamine resistance at two sites of different malaria transmission intensities in Rwanda

Author:
Kateera, Fredrick; Nsobya, Sam L.; Tukwasibwe, Steven; Hakizimana, Emmanuel; Mutesa, Leon; Mens, Petra F.; Grobusch, Martin P.; van Vugt, Michèle; Kumar, Nirbhay
Source:
Acta tropica 2016 v.164 pp. 329-336
ISSN:
0001-706X
Subject:
DNA; HIV infections; Human immunodeficiency virus; Plasmodium falciparum; bacterial infections; blood; chloroquine; disease control; drug resistance; drugs; exposure duration; fluorescence; genes; genetic polymorphism; genotyping; haplotypes; issues and policy; malaria; monitoring; mutants; parasites; patients; polymerase chain reaction; pregnant women; risk; selection pressure; Kenya; Malawi; Rwanda; Tanzania; Uganda
Abstract:
... Faced with intense levels of chloroquine (CQ) resistance in Plasmodium falciparum malaria, Rwanda replaced CQ with amodiaquine (AQ)+sulfadoxine-pyrimethamine (SP) in 2001, and subsequently with artemether–lumefantrine (AL) in 2006, as first-line treatments for uncomplicated malaria. Following years of discontinuation of CQ use, re-emergence of CQ-susceptible parasites has been reported in countrie ...
DOI:
10.1016/j.actatropica.2016.09.008

13. Occurency of Giardia duodenalis assemblages in river water sources of Black Sea, Turkey

Author:
Koloren, Zeynep; Seferoğlu, Onuralp; Karanis, Panagiotis
Source:
Acta tropica 2016 v.164 pp. 337-344
ISSN:
0001-706X
Subject:
DNA; Giardia lamblia; drinking water; genes; glutamate dehydrogenase; loop-mediated isothermal amplification; polymerase chain reaction; ribosomal RNA; river water; Black Sea; Turkey (country)
Abstract:
... A total of 420 environmental water samples and 120 drinking water samples from 45 different sampling sites of the Black Sea in Turkey were collected between 2012 and 2014. Genomic DNA was isolated from all the investigated water samples and comparativelly analyzed by Loop-mediated isothermal amplification (LAMP) of the elongation factor 1 Alfa (EF1α) gene, and by nested Polymerase Chain Reaction ( ...
DOI:
10.1016/j.actatropica.2016.09.025

14. Spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol

Author:
Kowalczyk, Agnieszka; Price, Oliver R.; van der Gast, Christopher J.; Finnegan, Christopher J.; van Egmond, Roger A.; Schäfer, Hendrik; Bending, Gary D.
Source:
Chemosphere 2016 v.164 pp. 355-362
ISSN:
0045-6535
Subject:
Pseudomonas syringae; bacterial communities; biodegradation; biodiversity; biofilm; biomass; chemical degradation; community structure; genes; inoculum; p-nitrophenol; quantitative polymerase chain reaction; river water; rivers; seasonal variation
Abstract:
... In order to predict the fate of chemicals in the environment, a range of regulatory tests are performed with microbial inocula collected from environmental compartments to investigate the potential for biodegradation. The abundance and distribution of microbes in the environment is affected by a range of variables, hence diversity and biomass of inocula used in biodegradation tests can be highly v ...
DOI:
10.1016/j.chemosphere.2016.08.095

15. Stereoselective induction of developmental toxicity and immunotoxicity by acetochlor in the early life stage of zebrafish

Author:
Xu, Chao; Tu, Wenqing; Deng, Mi; Jin, Yuanxiang; Lu, Bin; Zhang, Chaonan; Lin, Chunmian; Wu, Yongming; Liu, Weiping
Source:
Chemosphere 2016 v.164 pp. 618-626
ISSN:
0045-6535
Subject:
Danio rerio; Western blotting; abnormal development; acetochlor; aquatic environment; binding capacity; developmental stages; developmental toxicity; edema; enantiomers; fish; genes; immune system; immunotoxicity; innate immunity; interleukin-1beta; lethal concentration 50; median effective concentration; molecular dynamics; molecular models; mortality; protein synthesis; risk; stereochemistry; transcription (genetics); yolk sac
Abstract:
... Acetochlor (ACT) has been frequently detected in the aquatic environment and implicated in disruption of the immune system in fish, the mechanisms of which, especially at enantiomeric levels, remains unclear. In the present study, embryonic zebrafish were exposed to ACT and its enantiomers at concentrations of 0, 2, 8, 15, 30 and 60 μM from 2 h post-fertilization (hpf) to 72 hpf. We demonstrated t ...
DOI:
10.1016/j.chemosphere.2016.09.004

16. Structural and functional characterisation of FOXO/Acan-DAF-16 from the parasitic nematode Angiostrongylus cantonensis

Author:
Yan, Baolong; Sun, Weiwei; Yan, Lanzhu; Zhang, Liangliang; Zheng, Yuan; Zeng, Yuzhen; Huang, Huicong; Liang, Shaohui
Source:
Acta tropica 2016 v.164 pp. 125-136
ISSN:
0001-706X
Subject:
Caenorhabditis elegans; DNA repair; Parastrongylus cantonensis; amino acids; apoptosis; cell cycle; cell differentiation; free-living nematodes; gene expression; genes; longevity; mutation; neoplasms; nucleotide sequences; parasites; pharynx; phenotype; stress response; stress tolerance; transcription (genetics); transcription factors
Abstract:
... Fork head box transcription factors subfamily O (FoxO) is regarded to be significant in cell-cycle control, cell differentiation, ageing, stress response, apoptosis, tumour formation and DNA damage repair. In the free-living nematode Caenorhabditis elegans, the FoxO transcription factor is encoded by Ce-daf-16, which is negatively regulated by insulin-like signaling (IIS) and involved in promoting ...
DOI:
10.1016/j.actatropica.2016.09.011

17. The effects of rice-straw biochar addition on nitrification activity and nitrous oxide emissions in two Oxisols

Author:
He, Lili; Zhao, Xu; Wang, Shenqiang; Xing, Guangxi
Source:
Soil & tillage research 2016 v.164 pp. 52-62
ISSN:
0167-1987
Subject:
Oxisols; acid soils; ammonia; ammonium sulfate; application rate; bacteria; biochar; gene dosage; genes; granite; greenhouse gas emissions; greenhouse gases; kinetics; models; nitrates; nitrification; nitrous oxide; oxidants; quantitative polymerase chain reaction; rice straw; sandstone; soil pH
Abstract:
... Nitrification rates in Oxisols vary with soil pH and substrate availability. Biochar can be used to improve acid soils. The aim of this study was therefore to investigate the interactive impacts of 1% and 5% (w/w) rice-straw biochar application on nitrification, ammonia oxidizer populations and nitrous oxide (N2O) emissions over short periods of microcosm incubation in two agricultural Oxisols der ...
DOI:
10.1016/j.still.2016.05.006

18. The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions

Author:
Nikolić, Tatjana V.; Kojić, Danijela; Orčić, Snežana; Batinić, Darko; Vukašinović, Elvira; Blagojević, Duško P.; Purać, Jelena
Source:
Chemosphere 2016 v.164 pp. 98-105
ISSN:
0045-6535
Subject:
Apis mellifera; bioassays; cadmium; catalase; copper; dose response; environmental assessment; enzyme activity; gene expression; gene expression regulation; genes; glutathione; honey bees; lead; lipid peroxidation; pollution; superoxide dismutase; thiols
Abstract:
... In this study, laboratory bioassays were performed to investigate the impact of sublethal concentrations of Cu (CuCl2: 1000, 100, 10 mg L−1), Pb (PbCl2: 10, 1, 0.1 mg L−1) and Cd (CdCl2: 0.1, 0.01, 0.001 mg L−1) on honey bee redox status and the activity of the main antioxidative enzymes and their gene expression. Our results show that exposure to these metals led to significant changes of gene ex ...
DOI:
10.1016/j.chemosphere.2016.08.077

19. Transcriptional up-regulation of genes involved in photosynthesis of the Zn/Cd hyperaccumulator Sedum alfredii in response to zinc and cadmium

Author:
Tang, Lu; Yao, Aijun; Ming Yuan,; Tang, Yetao; Liu, Jian; Liu, Xi; Qiu, Rongliang
Source:
Chemosphere 2016 v.164 pp. 190-200
ISSN:
0045-6535
Subject:
RNA; Sedum; adenosine triphosphate; biomass; cadmium; carbonate dehydratase; chlorophyll; chloroplasts; electron transfer; fluorescence; gene expression regulation; genes; hyperaccumulators; leaves; metalloproteinases; photosystem I; photosystem II; plant growth; sequence analysis; transcription (genetics); translation (genetics); zinc
Abstract:
... Zinc (Zn) and cadmium (Cd) are two closely related chemical elements with very different biological roles in photosynthesis. Zinc plays unique biochemical functions in photosynthesis. Previous studies suggested that in some Zn/Cd hyperaccumulators, many steps in photosynthesis may be Cd tolerant or even Cd stimulated. Using RNA-seq data, we found not only that Cd and Zn both up-regulated the CA1 g ...
DOI:
10.1016/j.chemosphere.2016.08.026

20. Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells

Author:
Yang, Dandan; Chen, Shubin; Gao, Changzhao; Liu, Xiaobo; Zhou, Yulai; Liu, Pengfei; Cai, Jinglei
Source:
Life sciences 2016 v.164 pp. 9-14
ISSN:
0024-3205
Subject:
cartilage; chondrocytes; embryonic stem cells; genes; humans; in vivo studies; platelet-derived growth factor; quantitative polymerase chain reaction; staining; tissue transplantation
Abstract:
... The aim of this study was to improve a method that induce cartilage differentiation of human embryoid stem cells (hESCs) in vitro, and test the effect of in vivo environments on the further maturation of hESCs derived cells.Embryoid bodies (EBs) formed from hESCs, with serum-free KSR-based medium and mesodermal specification related factors, CHIR, and Noggin for first 8days. Then cells were digest ...
DOI:
10.1016/j.lfs.2016.09.004