Publication Year: 2016 / Source: 2016 v.36 no.3 / Subject: apples / Text Availability: Full Text

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Author:: Larsen, Bjarne; Ørgaard, Marian; Toldam-Andersen, Torben Bo; Pedersen, Carsten
Source:: Molecular breeding 2016 v.36 no.3 pp. 24
ISSN:: 1380-3743
Subject:: DNA; DNA primers; Malus domestica; alleles; apples; cultivars; diploidy; genotype; genotyping; polymerase chain reaction; ribonucleases; screening; triploidy
Abstract:: ... We present a new efficient screening tool for detection of S-alleles in apple. The protocol using general and multiplexed primers for PCR reaction and fragment detection on an automatized capillary DNA sequencer exposed a higher number of alleles than any previous studies. Analysis of alleles is made on basis of three individual fragment sizes making the allele interpretation highly accurate. The ...
DOI:: 10.1007/s11032-016-0448-0
PubMed:: 26941563
PubMed Central:: PMC4760992
: http://dx.doi.org/10.1007/s11032-016-0448-0