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You searched for: Publication year rev 7984-2016 Remove constraint Publication year rev: 7984-2016 Subject term Influenza A virus Remove constraint Subject term: Influenza A virus
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1. 6-(4-Amino-2-butyl-imidazoquinolyl)-norleucine: Toll-like receptor 7 and 8 agonist amino acid for self-adjuvanting peptide vaccine

Author:
Yoshio Fujita; Kazuyuki Hirai; Keigo Nishida; Hiroaki Taguchi
Source:
Amino acids 2016 v.48 no.5 pp. 1319-1329
ISSN:
0939-4451
Subject:
Influenza A virus; Toll-like receptor 7; Toll-like receptor 8; agonists; amino acids; antibodies; antigens; ligands; mice; peptides; pharmacology; subunit vaccines; vaccine adjuvants
Abstract:
... Generally, small peptides by themselves are weak to induce antibody responses. Toll-like receptor (TLR) ligands are attractive candidates of vaccine adjuvants to improve their antigenicity. The covalent conjugation of TLR ligands with antigens to produce self-adjuvanting peptide vaccine is a promising approach. Based on the structure of TLR7/8 ligands, a series of synthetic amino acids 6-imidazoqu ...
DOI:
10.1007/s00726-016-2190-7

2. A Balance between Inhibitor Binding and Substrate Processing Confers Influenza Drug Resistance

Author:
Li Jiang; Ping Liu; Claudia Bank; Nicholas Renzette; Kristina Prachanronarong; Lutfu S. Yilmaz; Daniel R. Caffrey; Konstantin B. Zeldovich; Celia A. Schiffer; Timothy F. Kowalik; Jeffrey D. Jensen; Robert W. Finberg; Jennifer P. Wang; Daniel N.A. Bolon
Source:
Journal of Molecular Biology 2016 v.428 pp. 538-553
ISSN:
0022-2836
Subject:
Influenza A virus; active sites; drug resistance; drugs; hydrophobicity; influenza; mutation; oseltamivir; sialidase
Abstract:
... The therapeutic benefits of the neuraminidase (NA) inhibitor oseltamivir are dampened by the emergence of drug resistance mutations in influenza A virus (IAV). To investigate the mechanistic features that underlie resistance, we developed an approach to quantify the effects of all possible single-nucleotide substitutions introduced into important regions of NA. We determined the experimental fitne ...
DOI:
10.1016/j.jmb.2015.11.027

3. A Dose-Response Study of Inactivated Low Pathogenic Avian Influenza H9N2 Virus in Specific-Pathogen-Free and Commercial Broiler Chickens

Author:
Kilany Walid H.; Ali Ahmed; Bazid Abdel-Hamid I.; El-Deeb Ayman H.; El-Abideen Mohamed A. Zain; Sayed Magdy El; El-Kady Magdy F.
Source:
Avian diseases 2016 v.60 no.1s pp. 256-261
ISSN:
0005-2086
Subject:
Influenza A virus; antibodies; antigens; avian influenza; broiler chickens; dose response; financial economics; hemagglutination; poultry industry; poultry production; specific pathogen-free animals; vaccination; vaccines; viral shedding; viruses; Egypt
Abstract:
... Since the first report of low pathogenic avian influenza (LPAI) H9N2 virus in Egypt in 2011, the Egyptian poultry industry has suffered from unexpected economic losses as a result of the wide spread of LPAI H9N2. Hence, inactivated H9N2 vaccines have been included in the vaccination programs of different poultry production sectors. The optimal antigen content of avian influenza virus vaccines is e ...
DOI:
10.1637/11143-050815-Reg

4. A Novel Optical Biosensing System Using Mach–Zehnder-Type Optical Waveguide for Influenza Virus Detection

Author:
Hiroaki Sakamoto; Yuma Minpou; Takayuki Sawai; Yasufumi Enami; Shin-ichiro Suye
Source:
Applied biochemistry and biotechnology 2016 v.178 no.4 pp. 687-694
ISSN:
0273-2289
Subject:
Influenza A virus; antibodies; biosensors; cross-linking reagents; disease outbreaks; glass; influenza; porosity; silica; viruses; wavelengths
Abstract:
... In order to minimize the damage from viral epidemics, early detection of the causative agent of a viral epidemic and prevention of its immediate spread are urgent social demands. Therefore, in this study, we evaluated the utility of a Mach–Zehnder-type optical waveguide as a sensing device for influenza virus detection. However, it is impossible to detect a 100-nm-size virus using a sol-gel optica ...
DOI:
10.1007/s12010-015-1902-x

5. A Refined Guinea Pig Model of Foot‐and‐Mouth Disease Virus Infection for Assessing the Efficacy of Antiviral Compounds

Author:
A. R. De Vleeschauwer; D. J. Lefebvre; T. Willems; G. Paul; A. Billiet; L. E. Murao; J. Neyts; N. Goris; K. De Clercq
Source:
Transboundary and emerging diseases 2016 v.63 no.2 pp. e205
ISSN:
1865-1674
Subject:
Foot-and-mouth disease virus; Influenza A virus; RNA; antiviral agents; antiviral properties; blood serum; cattle; foot-and-mouth disease; guinea pigs; in vivo studies; models; oral administration; vaccination; vaccines; virus replication; Europe
Abstract:
... An antiviral containment strategy for foot‐and‐mouth disease (FMD) outbreaks could support or replace current contingency plans in case of an outbreak in Europe and could spare many healthy animals from being pre‐emptively culled. Recently, substantial progress has been made towards the development of small molecule drugs that inhibit FMD virus (FMDV) replication in vitro. For the initial in vivo ...
DOI:
10.1111/tbed.12255

6. A Simulation-Based Evaluation of Premovement Active Surveillance Protocol Options for the Managed Movement of Turkeys to Slaughter During an Outbreak of Highly Pathogenic Avian Influenza in the United States

Author:
Todd Weaver J.; Malladi Sasidhar; Bonney Peter J.; Patyk Kelly A.; Bergeron Justin G.; Middleton Jamie L.; Alexander Catherine Y.; Goldsmith Timothy J.; Halvorson David A.
Source:
Avian diseases 2016 v.60 no.1s pp. 132-145
ISSN:
0005-2086
Subject:
Influenza A virus; avian influenza; biosecurity; business enterprises; disease models; flocks; genes; monitoring; reverse transcriptase polymerase chain reaction; risk; risk assessment; risk management; simulation models; slaughter; stakeholders; turkeys; United States
Abstract:
... Risk management decisions associated with live poultry movement during a highly pathogenic avian influenza (HPAI) outbreak should be carefully considered. Live turkey movements may pose a risk for disease spread. On the other hand, interruptions in scheduled movements can disrupt business continuity. The Secure Turkey Supply (STS) Plan was developed through an industry-government-academic collabor ...
DOI:
10.1637/11108-042415-Reg

7. A Systematic Review of the Comparative Epidemiology of Avian and Human Influenza A H5N1 and H7N9 – Lessons and Unanswered Questions

Author:
C. Bui; A. Bethmont; A. A. Chughtai; L. Gardner; S. Sarkar; S. Hassan; H. Seale; C. R. MacIntyre
Source:
Transboundary and emerging diseases 2016 v.63 no.6 pp. 602-620
ISSN:
1865-1674
Subject:
Influenza A virus; emerging diseases; epidemiology; farms; human influenza; human population; humans; migratory behavior; monitoring; poultry; poultry industry; respiratory system; systematic review; wild birds; China
Abstract:
... The aim of this work was to explore the comparative epidemiology of influenza viruses, H5N1 and H7N9, in both bird and human populations. Specifically, the article examines similarities and differences between the two viruses in their genetic characteristics, distribution patterns in human and bird populations and postulated mechanisms of global spread. In summary, H5N1 is pathogenic in birds, whi ...
DOI:
10.1111/tbed.12327

8. A cell culture-derived whole virus influenza A vaccine based on magnetic sulfated cellulose particles confers protection in mice against lethal influenza A virus infection

Author:
Michael M. Pieler; Sarah Frentzel; Dunja Bruder; Michael W. Wolff; Udo Reichl
Source:
Vaccine 2016 v.34 no.50 pp. 6367-6374
ISSN:
0264-410X
Subject:
Influenza A virus; antibodies; bioreactors; cellulose; dogs; genes; immunity; influenza; influenza vaccines; kidneys; lungs; magnetism; mice; nucleoproteins; protocols; screening; vaccination; virion; viruses
Abstract:
... Downstream processing and formulation of viral vaccines employs a large number of different unit operations to achieve the desired product qualities. The complexity of individual process steps involved, the need for time consuming studies towards the optimization of virus yields, and very high requirements regarding potency and safety of vaccines results typically in long lead times for the establ ...
DOI:
10.1016/j.vaccine.2016.10.041

9. A duplex RT-PCR assay for detection of H9 subtype avian influenza viruses and infectious bronchitis viruses

Author:
Yan-di WEI; Wei-hua GAO; Hong-lei SUN; Chen-fang YU; Xing-yao PEI; Yi-peng SUN; Jin-hua LIU; Juan PU
Source:
Journal of integrative agriculture 2016 v.15 no.9 pp. 2105-2113
ISSN:
2095-3119
Subject:
DNA primers; Infectious bronchitis virus; Influenza A virus; chickens; egg production; eggs; financial economics; genes; hemagglutinins; infectious bronchitis; mixed infection; monitoring; nucleocapsid; pathogens; respiratory tract diseases; reverse transcriptase polymerase chain reaction; viruses; China
Abstract:
... H9 subtype avian influenza virus (AIV) and infectious bronchitis virus (IBV) are major pathogens circulating in poultry and have resulted in great economic losses due to respiratory disease and reduced egg production. As similar symptoms are elicited by the two pathogens, it is difficult for their differential diagnosis. So far, no reverse transcription-polymerase chain reaction (RT-PCR) assay has ...
DOI:
10.1016/S2095-3119(15)61316-8
PubMed:
32288952
PubMed Central:
PMC7128909

10. A high-throughput screening system targeting the nuclear export pathway via the third nuclear export signal of influenza A virus nucleoprotein

Author:
Michinori Kakisaka; Takafumi Mano; Yoko Aida
Source:
Virus research 2016 v.217 pp. 23-31
ISSN:
0168-1702
Subject:
Aequorea; Influenza A virus; antiviral agents; cell lines; green fluorescent protein; image analysis; influenza; nucleoproteins; protein transport; screening; sialidase; viral proteins; virus replication
Abstract:
... Two classes of antiviral drugs, M2 channel inhibitors and neuraminidase (NA) inhibitors, are currently approved for the treatment of influenza; however, the development of resistance against these agents limits their efficacy. Therefore, the identification of new targets and the development of new antiviral drugs against influenza are urgently needed. The third nuclear export signal (NES3) of nucl ...
DOI:
10.1016/j.virusres.2016.02.007

11. A highly immunogenic vaccine against A/H7N9 influenza virus

Author:
Weiping Cao; Justine S. Liepkalns; Ahmed O. Hassan; Ram P. Kamal; Amelia R. Hofstetter; Samuel Amoah; Jin Hyang Kim; Adrian J. Reber; James Stevens; Jacqueline M. Katz; Shivaprakash Gangappa; Ian A. York; Suresh K. Mittal; Suryaprakash Sambhara
Source:
Vaccine 2016 v.34 no.6 pp. 744-749
ISSN:
0264-410X
Subject:
Influenza A virus; animal models; antibodies; at-risk population; blood serum; cell-mediated immunity; hemagglutinins; human diseases; influenza; pandemic; vaccines; viruses
Abstract:
... Since the first case of human infection in March 2013, continued reports of H7N9 cases highlight a potential pandemic threat. Highly immunogenic vaccines to this virus are urgently needed to protect vulnerable populations who lack protective immunity. In this study, an egg- and adjuvant-independent adenoviral vector-based, hemagglutinin H7 subtype influenza vaccine (HAd-H7HA) demonstrated enhanced ...
DOI:
10.1016/j.vaccine.2015.12.062
PubMed:
26765287
PubMed Central:
PMC4735641

12. A membrane-based purification process for cell culture-derived influenza A virus

Author:
Thomas Weigel; Thomas Solomaier; Sebastian Wehmeyer; Alessa Peuker; Michael W. Wolff; Udo Reichl
Source:
Journal of biotechnology 2016 v.220 pp. 12-20
ISSN:
0168-1656
Subject:
DNA; Influenza A virus; anion exchange; binding capacity; cellulose; chromatography; digestion; humans; production technology; purification methods; salt tolerance; vaccines; viruses
Abstract:
... A simple membrane-based purification process for cell culture-derived influenza virus was established that relies on only two chromatographic unit operations to achieve the contamination limits required according to regulatory authorities. After clarification and concentration, a pseudo-affinity membrane adsorber (sulfated cellulose, SCMA) was applied for virus capture. The subsequent polishing st ...
DOI:
10.1016/j.jbiotec.2015.12.022

13. A modified strategy of multiplex RT-PCR for simultaneous detection of H5, H7, and H9 subtypes of avian influenza virus based on common forward oligo

Author:
M.S. Tahir; D. Mehmood; A.U. Sultan; M.H. Saeed; A.R. Khan; F. Ansari; M.M. Salman; K.A. Majeed
Source:
Journal of applied poultry research 2016 v.25 no.3 pp. 322-327
ISSN:
1056-6171
Subject:
Influenza A virus; agarose; birds; diagnostic techniques; disease prevention; gels; genotyping; industry; poultry; research; reverse transcriptase polymerase chain reaction; vaccination; Pakistan
Abstract:
... Avian influenza virus subtypes H₅, H₇, and H₉ related epidemics are accountable for huge losses to the poultry and avian industry in Pakistan. Well-timed and accurate diagnosis of subtype(s) concomitant with a specific epidemic allows a margin of prophylaxis to farmers through requisite vaccination. The current study was designed to develop and validate a rapid, accurate, and efficient diagnostic ...
DOI:
10.3382/japr/pfw014

14. A natural component from Euphorbia humifusa Willd displays novel, broad-spectrum anti-influenza activity by blocking nuclear export of viral ribonucleoprotein

Author:
So Young Chang; Ji Hoon Park; Young Ho Kim; Jong Seong Kang; Ji-Young Min
Source:
Biochemical and biophysical research communications 2016 v.471 pp. 282-289
ISSN:
0006-291X
Subject:
Euphorbia; Influenza A virus; cytoplasm; dose response; drug resistance; fluorescent antibody technique; influenza; mechanism of action; mutation; nucleoproteins; oseltamivir; physiological transport; progeny; ribonucleoproteins; screening; sialidase; signal transduction; virion; viruses
Abstract:
... The need to develop anti-influenza drugs with novel antiviral mechanisms is urgent because of the rapid rate of antigenic mutation and the emergence of drug-resistant viruses. We identified a novel anti-influenza molecule by screening 861 plant-derived natural components using a high-throughput image-based assay that measures inhibition of the influenza virus infection. 1,3,4,6-tetra-O-galloyl-β-D ...
DOI:
10.1016/j.bbrc.2016.01.123

15. A novel p38 mitogen activated protein kinase (MAPK) specific inhibitor suppresses respiratory syncytial virus and influenza A virus replication by inhibiting virus-induced p38 MAPK activation

Author:
Myung-Soo Choi; Jinyuk Heo; Chae-Min Yi; Junsu Ban; Noh-Jin Lee; Na-Rae Lee; Sang Won Kim; Nam-Jung Kim; Kyung-Soo Inn
Source:
Biochemical and biophysical research communications 2016 v.477 pp. 311-316
ISSN:
0006-291X
Subject:
Influenza A virus; epithelial cells; influenza; interleukin-6; messenger RNA; mitogen-activated protein kinase; people; secretion; toxicity; virus replication; viruses
Abstract:
... Respiratory syncytial virus (RSV) and influenza A virus are leading causes of acute lower respiratory infectious disease. Respiratory diseases caused by RSV and influenza A virus result in serious economic burden and life-threatening disease for immunocompromised people. With the revelation that p38 mitogen-activated protein kinase (MAPK) activity in host cells is crucial for infection and replica ...
DOI:
10.1016/j.bbrc.2016.06.111

16. A point mutation in the polymerase protein PB2 allows a reassortant H9N2 influenza isolate of wild-bird origin to replicate in human cells

Author:
Islam T.M. Hussein; Eric J. Ma; Nichola J. Hill; Brandt W. Meixell; Mark Lindberg; Randy A. Albrecht; Justin Bahl; Jonathan A. Runstadler
Source:
Infection, genetics, and evolution 2016 v.41 pp. 279-288
ISSN:
1567-1348
Subject:
Influenza A virus; amino acid substitution; amino acids; ancestry; genes; genetic polymorphism; genetic techniques and protocols; hosts; humans; influenza; pandemic; pathogenicity; phenotype; point mutation; screening; viruses; wild birds; North America
Abstract:
... H9N2 influenza A viruses are on the list of potentially pandemic subtypes. Therefore, it is important to understand how genomic reassortment and genetic polymorphisms affect phenotypes of H9N2 viruses circulating in the wild bird reservoir. A comparative genetic analysis of North American H9N2 isolates of wild bird origin identified a naturally occurring reassortant virus containing gene segments ...
DOI:
10.1016/j.meegid.2016.04.011
PubMed:
27101787
PubMed Central:
PMC4868792

17. A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs

Author:
Marie Borggren; Jens Nielsen; Ingrid Karlsson; Tina S. Dalgaard; Ramona Trebbien; James A. Williams; Anders Fomsgaard
Source:
Vaccine 2016 v.34 no.32 pp. 3634-3640
ISSN:
0264-410X
Subject:
DNA; Influenza A virus; antibiotic resistance; cell-mediated immunity; cross immunity; cross reaction; dose response; electroporation; ferrets; genes; hemagglutinins; herds; hosts; nucleoproteins; pandemic; public health; recombinant vaccines; swine; swine influenza; vaccination; viruses
Abstract:
... Pigs are natural hosts for influenza A viruses, and the infection is widely prevalent in swine herds throughout the world. Current commercial influenza vaccines for pigs induce a narrow immune response and are not very effective against antigenically diverse viruses. To control influenza in pigs, the development of more effective swine influenza vaccines inducing broader cross-protective immune re ...
DOI:
10.1016/j.vaccine.2016.05.030
PubMed:
27211039
PubMed Central:
PMC4940207

18. Abiotic stress of ambient cold temperature regulates the host receptivity to pathogens by cell surfaced sialic acids

Author:
Seong-Cheol Moon; Su-Yeon Joo; Tae-Wook Chung; Hee-Jung Choi; Mi-Ju Park; Hee-Jin Choi; Sung-Jin Bae; Keuk-Jun Kim; Cheorl-Ho Kim; Myungsoo Joo; Ki-Tae Ha
Source:
Biochemical and biophysical research communications 2016 v.476 pp. 159-166
ISSN:
0006-291X
Subject:
Influenza A virus; acids; cold; cold stress; cultured cells; epithelium; hemagglutinins; humans; lungs; messenger RNA; mice; pathogens; sialic acids; temperature; viruses
Abstract:
... Ambient cold temperature, as an abiotic stress, regulates the survival, stability, transmission, and infection of pathogens. However, the effect of cold temperature on the host receptivity to the pathogens has not been fully studied. In this study, the expression of terminal α-2,3- and α-2,6-sialic acids were increased in murine lung tissues, especially bronchial epithelium, by exposure to cold co ...
DOI:
10.1016/j.bbrc.2016.05.020

19. Activation of cross-reactive mucosal T and B cell responses in human nasopharynx-associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines

Author:
Jennifer Mullin; Muhammed S. Ahmed; Ravi Sharma; Navdeep Upile; Helen Beer; Priya Achar; Suttida Puksuriwong; Francesca Ferrara; Nigel Temperton; Paul McNamara; Teresa Lambe; Sarah C. Gilbert; Qibo Zhang
Source:
Vaccine 2016 v.34 no.14 pp. 1688-1695
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; Influenza A virus; antibodies; antigens; hemagglutinins; humans; immunity; influenza; nucleoproteins; pandemic; vaccine development; vaccines; vertebrate viruses
Abstract:
... Recent efforts have been focused on the development of vaccines that could induce broad immunity against influenza virus, either through T cell responses to conserved internal antigens or B cell response to cross-reactive haemagglutinin (HA). We studied the capacity of Modified Vaccinia Ankara (MVA)-vectored influenza vaccines to induce cross-reactive immunity to influenza virus in human nasophary ...
DOI:
10.1016/j.vaccine.2016.02.028

20. Adaptive amino acid substitutions enhance the virulence of a novel human H7N9 influenza virus in mice

Author:
Yongkun Zhao; Zhijun Yu; Linna Liu; Tiecheng Wang; Weiyang Sun; Chengyu Wang; Zhiping Xia; Yuwei Gao; Bo Zhou; Jun Qian; Xianzhu Xia
Source:
Veterinary microbiology 2016 v.187 pp. 8-14
ISSN:
0378-1135
Subject:
Influenza A virus; amino acid substitution; amino acids; genome; humans; mice; proteins; virulence; viruses
Abstract:
... To identify molecular features that confer enhanced H7N9 virulence in mammals, we independently generated three mouse-adapted variants of A/Shanghai/2/2013 (H7N9) by serial passage in mice. The mouse lethal doses (MLD50) of the mouse-adapted variants were reduced >1000–100000-fold when compared to the parental virus. Adapted variants displayed enhanced replication kinetics in vivo, and were capabl ...
DOI:
10.1016/j.vetmic.2016.02.027