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... Combined pretreatment methods for improving sugar yield as well as the economic aspects of lignoethanol production processes have gained a great deal of interest. However, most investigations involving such methods have been conducted under optimum conditions. Such fully-optimized combinations although improve enzymatic digestion, they could also degrade cellulose structure resulting in reduced su ...
... An innovative integrated biorefinery approach using the green macroalgae Chaetomorpha linum was investigated in the present study for the co-production of bioethanol and biogas. Among three pretreatments of C. linum biomass, consisting of acidic, neutral and alkali ones, 3% NaOH pretreatment gave the best result in terms of thallus disintegration, biomass recovery and enzymatic digestibility as de ...
... This study proposes a simple and fast sample treatment to determine cadmium levels in meat samples (edible offal) of several animal species by graphite furnace atomic absorption spectrometry, GF AAS, as an alternative to the calcination method, currently employed by Official Food Control department of the Brazilian Ministry of Agriculture, Livestock, and Food Supply (MAPA). In the proposed method ...
alkali treatment; ambient temperature; capacitance; commercialization; electrodes; energy; energy density; graphene; graphene oxide; porous media
Abstract:
... The low energy density of graphene-based supercapacitors has been a critical bottleneck for their commercialization. In particular, the poor capacitance due to the lack of electrically porous channels and insufficient Faradaic reactions in reduced graphene oxide (RGO) electrodes has been a major limiting factor for their successful application. Herein, we demonstrate an innovative process for modi ...
acetic acid; acidification; activated sludge; alkali treatment; anaerobic digestion; biogas; chemical oxygen demand; energy; fermentation; hydrolysis; intermediate product; methane; methane production; pH; short chain fatty acids; temperature
Abstract:
... As an important intermediate product, short-chain fatty acids (SCFAs) can be generated after hydrolysis and acidification from waste activated sludge, and then can be transformed to methane during anaerobic digestion process. In order to obtain more SCFA and methane, most studies in literatures were centered on enhancing the hydrolysis of sludge anaerobic digestion which was proved as un-efficient ...
... A novel stepwise pretreatment on corn stalk (CS) by alkali deacetylation combined with liquid hot water (LHW) was investigated to enhance enzymatic hydrolysis. After deacetylated treatment, strength of alkali deacetylation of CS was from 1.79% to 91.34% which was subsequently pretreated by LHW with severity from 3.27 to 4.27. It was found that higher strength of alkali deacetylation could reduce b ...
... The conversion of paper-grade pulps into dissolving pulps requires efficient strategies and process steps to remove low-molecular noncellulosic macromolecules generally known as hemicelluloses. Current strategies include alkaline extractions and enzymatic treatments. This study focused on the evaluation of extraction efficiencies in alkaline extractions of three economically interesting hardwood s ...
... The native form of lignocellulosic biomass is resistant to enzymatic breakdown. A well-designed pretreatment that can promote enzymatic hydrolysis of biomass with reasonable processing cost is therefore necessary. To this end, a number of different types of pretreatment technologies have been developed with a common goal of making biomass more susceptible to enzymatic saccharification. Among those ...
Lívia Beatriz Brenelli; Fernanda Mandelli; Adriana Zerlotti Mercadante; George Jackson de Moraes Rocha; Silvana Aparecida Rocco; Aldo Felix Craievich; Adilson Roberto Gonçalves; Danilo da Cruz Centeno; Mario de Oliveira Neto; Fabio Marcio Squina
... Lignin constitutes up to one-third of the material found in plant cell walls and is considered the second most abundant natural polymer in the world. Despite unique characteristics of lignin, it is mostly used for low-value commercial applications. In this study, lignin obtained after alkaline treatment of steam-exploded sugarcane bagasse was submitted to an acidification process. The soluble frac ...
... Bioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implement ...
... This study investigates alkali-solubilized dissolved organic matter (DOM) and its fate in the anaerobic treatment process. DOM was fractionated into high molecular weight (HMW) protein-like substances (PL), HMW saccharide-like substances (SL), low molecular weight (LMW) PL, LMW SL, and humic acid-like substances (HAL). The results indicate alkali-solubilized DOM is primarily composed of LMW PL, HM ...
... This research evaluates the addition of alkaline amendments to enhance the degradation of polylactic acid polymers (PLA) in compost conditions. The use of compostable biopolymers is increasing in part because they are advertised as compostable. However, PLA degrades slowly compared to the organic wastes in compost and even when processed in commercial composting facilities may not fully break down ...
Viviane Marcos Nascimento; Anny Manrich; Paulo Waldir Tardioli; Roberto de Campos Giordano; George Jackson de Moraes Rocha; Raquel de Lima Camargo Giordano
... The economics for production of secondgeneration (2G) ethanol from sugarcane bagasse in large scale, competing with the cogeneration of electric energy, is still not consolidated. In this scenario, the key for feasibility may be the biorefinery concept, a multiproduct industry using biomass fractions to produce energy, chemicals and by-products. Xylooligosaccharides (XOS) are oligomers of xylose o ...
... Commercial table eggs are washed prior to packaging. Standard wash procedures use an alkaline pH and warm water. If a cool water method could be developed that would still provide a microbiologically safe egg, the industry may save energy costs associated with water heating. Four wash procedures were evaluated for Salmonella reduction: pH 11 at 48.9°C (industry standard), pH 11 at ambient temperat ...
... The colloid of graphitic carbon nitride (g-C₃N₄) was of great importance for practical application. Herein we introduced an alkali treatment route to efficiently colloidize g-C₃N₄ under mild conditions by destroying the hydrogen bonds between linearly polymeric melon chains and hydrolyzing partial CNHC bonds linked two tri-s-triazine units. The obtained colloidal suspension was extremely stable du ...
... Effluent sludge from an anaerobic digester was used as a source of nitrogen, phosphate, sulfur, and other nutrients in the culture medium of ethanol production by the yeast Saccharomyces cerevisiae. Several pretreatments (mechanical, chemical, thermal, and thermo-chemical) were performed on the anaerobic digested sludge (ADS) to make the nutrients accessible to the yeast cells. Preliminary experim ...
... Abundant organic compounds in textile dyeing sludge (TDS) provide possibility for its anaerobic digestion (AD) treatment. However, preliminary test showed little biogas generation in direct AD of the TDS during 20days. In order to improve the AD availability of TDS, alkaline, acid, thermal and thermal alkaline pretreatments were performed. Color and aromatic amines were specifically measured as ex ...
... In this study, five different kinds of polysaccharides (AAP1, AAP2, AAP3, AAP4, and AAP5) were extracted from different varieties of Auricularia auricula through an alkali extraction process. Furthermore, the crude polysaccharides were deproteinized by the Sevag method. Auricularia auricula produced in the Shanxi province had the highest content of polysaccharide, 53.02%. The monosaccharide compos ...
Cannabis sativa; alkali treatment; antioxidants; bioactive properties; enzymatic hydrolysis; hemp; hydrolysates; hydrolysis; hydrophobicity; particle size; polyacrylamide gel electrophoresis; protein hydrolysates; protein isolates; proteinases; zeta potential
Abstract:
... Hemp protein isolates (HPIs) were hydrolysed by proteases (AFP, HT, ProG, actinidin and zingibain). The enzymatic hydrolysis of HPIs was evaluated through the degree of hydrolysis and SDS-PAGE profiles. The bioactive properties of the resultant hydrolysates (HPHs) were accessed through ORAC, DPPḢ scavenging and ACE-inhibitory activities. The physical properties of the resultant HPHs were evaluated ...
... The aim of the present work was to examine if hydrogen peroxide treatment of sugar beet fibre that aimed at improving its physicochemical properties would impair its antioxidant potential. Three different sugar beet fibres were obtained from sugar beet – non‐treated fibre (NTF) from sugar beet cossettes extracted with sulphurous acid, treated fibre (TF) from NTF treated with hydrogen peroxide in a ...
... The effectiveness of slightly acidic electrolyzed water (SAEW) in reducing Escherichia coli, Salmonella typhimurim, Staphylococcus aureus or bacterial mixtures on stainless steel surfaces was evaluated and compared its efficacy with composite phenol solution for reducing total aerobic bacteria in animal transport vehicles. Stainless steel surfaces were inoculated with these strains individually or ...
... Efficient utilization of a wide range of feedstocks requires appropriate biorefining strategies based on pretreatment methods. This study showed that alkali pretreatment was significantly more effective than acid pretreatment at similar processing conditions for grass and hardwood biomass samples; but, both methods were ineffective for softwood biomass. Separate glucose and xylose streams can be o ...
... The high methane gas production potential of two phase olive milling waste (2POMW) makes its application to biogas plants in business an economical process to increase the productivity of the plants. The objective of this study was to investigate the appropriate conditions for the codigestion of NaOH-pretreated 2POMW with food waste. NaOH pretreatment can increase the methane production by increas ...
... The dipping of berries in a dilute solution of sodium hydroxide during a short time was evaluated as pretreatment undertaken prior to convective dehydration of wine grapes. The impact of the sodium hydroxide content and dipping time on weight loss (WL) at different dehydration times was thoroughly assessed using central composite design (CCD) and response surface methodology (RSM). Furthermore, th ...
... The impact of alkaline pretreatment at different alkaline charges (5%, 10% and 15% NaOH w/w, on dry basis) on the chemical composition of eucalyptus, sugarcane bagasse and straw were compared with the subsequent bioconversion into ethanol, using semi-simultaneous saccharification and fermentation (SSSF). By increasing alkaline charge in pretreatments, substantial amount of lignin, hemicelluloses a ...
Bacillus (bacteria); acid treatment; activated sludge; adsorption; alkali treatment; aqueous solutions; bacteria; biomass; biosorbents; biosorption; cadmium; chromium; heavy metals; industrial wastes; industrial wastewater; ion exchange; lead; manganese; metal ions; metal tolerance; toxicity; waste treatment
Abstract:
... The microorganisms are capable of accumulating heavy metal ions from water as biosorbent agents, offering a potential alternative for the detoxification and recovery of toxic/precious metals in industrial wastewater. In the present work, metal-resistant bacterial strains were isolated and identified from activated sludge of a waste treatment plant in the Municipality of Santa Rosa Jauregui, Querét ...
... In the present study, the bactericidal and virucidal mechanisms in the alkaline disinfection of compost with calcium lime and ash were investigated. Two indicator microorganisms, Escherichia coli and MS2 coliphage, were used as surrogates for enteric pathogens. The alkaline-treated compost with calcium oxide (CaO) or ash resulted primarily in damage to the outer membrane and enzyme activities of E ...
... A novel series of oxy-diester-functionalized gemini surfactants (Cₘ-E2O-Cₘ) was synthesized and the physicochemical parameters were evaluated by surface tension and steady-state fluorescence measurements. Negative values of Gibbs free energy change of adsorption and micellization indicate that adsorption and micellization of Cₘ-E2O-Cₘ gemini surfactants are spontaneous. These surfactants have lowe ...
... The production of ethanol from the fast-growing perennial C4 grass Miscanthus floridulus by simultaneous saccharification and fermentation (SSF) was investigated. M. floridulus biomass was composed of 36.3% glucan, 22.8% hemicellulose, and 21.3% lignin (based on dried mass). Prior to SSF, harvested stems of M. floridulus were pretreated separately by alkali treatment at room temperature, alkali tr ...
alkali treatment; degumming; fibroins; physical properties; proteolysis; scanning electron microscopy; sericin; silk; silk fabric; staining; subtilisin; tensile strength; weight loss
Abstract:
... The biological degumming of silk fibers was studied using alkaline proteases such as Savinase and Alcalase, comparing with conventional alkaline treatments. Degumming efficiency was evaluated by measuring the residual silk sericin in terms of weight loss and surface staining with reactive dye. The water absorbency and tensile strength were measured to characterize surface and physical properties, ...
... Recent techno-economic studies of proposed lignocellulosic biorefineries have concluded that creating value from lignin will assist realization of biomass utilization into valuable fuels, chemicals, and materials due to co-valorization and the new revenues beyond carbohydrates. The pretreatment step within a biorefinery process is essential for recovering carbohydrates, but different techniques an ...
Methanosarcina; alkali treatment; anaerobic digestion; biogas; community structure; denaturing gradient gel electrophoresis; digestion; hemicellulose; industrial wastes; lignocellulose; methane; methanogens; microarray technology; organic matter; paper; papermaking; pulp and paper industry; quantitative polymerase chain reaction; temperature; ultrasonics; wood pulp
Abstract:
... BACKGROUND: Cellulose-containing waste products from the agricultural or industrial sector are potentially one of the largest sources of renewable energy on earth. In this study, the biomethane potential (BMP) of two types of industrial paper wastes, wood and pulp residues (WR and PR, respectively), were evaluated under both mesophilic and thermophilic conditions, and various pretreatment methods ...
... Oil palm frond (OPF) bagasse, being one of the cheapest biomass ever reported ($19.4/t), was hydrolyzed and used as a raw material for fermentative production of succinic acid for the first time. Results indicated that glucose and xylose were released as the main carbon source after alkaline pretreatment followed by enzymatic hydrolysis. The resultant hydrolysate from the pretreated OPF bagasse wa ...
... An integrated biorefinery process based on acidic leaching, alkaline treatment and concentrated sulfuric acid hydrolysis was set up for the simultaneous recovery of lignin, hemicelluloses, silica, and cellulose nanocrystals (CNCs) from Arundo donax and rice husk. These fractions can serve as viable source of bio-chemicals with potential high-value applications. A chromatographic and spectroscopic ...
... The biosorption of phosphorus in the form of orthophosphate (Po) from wastewater using biomass as the sorbent is of potential importance because the Po-loaded biomass could be applied in the agricultural sector as fertilizer and soil conditioner. However, biomass generally displays a very low affinity for Po sorption and therefore biomass surface modification is required. In the present study, the ...
... Sugarcane bagasse (SB) is a potential feedstock for butanol production. However, biological production of butanol from SB is less economically viable. In this study, evaluation of eight pretreatments on SB showed that alkali pretreatment efficiently removed lignin from SB while retaining the intact native structure of the released microfibrils. In total, 99% of cellulose and 100% of hemicellulose ...
... Aqueous extraction of cystocarpic, tetrasporic, and vegetative individuals of Mazzaella laminariodes collected in the Magellan Ecoregion, Southern Chile, afforded sulfated galactans. Analysis by Fourier transform infrared spectroscopy (FT-IR) and by ¹H and ¹³C NMR spectroscopy indicated that purified extracts from cystocarpic (CP) and vegetative individuals (VP) were mixtures of κ/ι-type carrageen ...
... Lignocellulosic bioethanol is an important renewable fuel for transportation purpose. Commercial production of lignocellulosic bioethanol mainly depends on cost of cellulase production, efficient pretreatment and enzymatic hydrolysis process. In the present study cellulase production from Aspergillus niger under submerged fermentation (SmF) was optimized using coconut water as natural medium. Maxi ...
... Gelatins extracted from the swim bladder of yellowfin tuna (Thunnus albacores) using various alkaline pretreatments were characterized. Alkaline mixtures (Na ₂CO ₃:NaOH) at different ratios (9:1, 8:2, 7:3, and 6:4) with a concentration of 4% (w/v) were used. The corresponding gelatins termed “G1,” “G2,” “G3,” and “G4” had yields of 9.78, 14.91, 35.96, and 13.60% (dry weight basis), respectively. A ...
... Sugarcane culm has different types of tissue organization, and its heterogeneity can influence the bagasse quality generated in the sugar/ethanol industry. The heterogeneity of the sugarcane bagasse contributes to the intrinsic recalcitrance of the biomass, impairing its conversion to ethanol. To determine the effects of sugarcane culm heterogeneity, the internode and node fractions were pretreate ...
... Four types of kenaf bast fibers were prepared via a combination of Wise treatments, for delignification, and alkaline treatments, for the removal of hemicellulose. Each type of kenaf bast fiber with different refining processes were nano fibrillated by grinding. Resulting, cellulose nanofiber (CNF) sheet was obtained from CNF by vacuum filtration (Scheme 1). The structures and properties of these ...
alkali treatment; amino acid composition; centrifugation; chemical precipitation; cold; cost effectiveness; denaturation; gelatinization; industry; ingredients; protein concentrates; protein content; rice; rice flour; rice starch; sodium hydroxide; starch
Abstract:
... This study evaluated the characteristics of rice starch and protein obtained by a fast alkaline extraction method on rice flour (RF) derived from broken rice. The extraction was conducted using 0.18% NaOH at 30°C for 30min followed by centrifugation to separate the starch rich and the protein rich fractions. This fast extraction method allowed to obtain an isoelectric precipitation protein concent ...
... Improvement of enzymatic hydrolysis and ethanol production from softwood pine was conducted by pretreatment with 8% (w/v) NaOH at different temperatures of 0, 25, and 80 °C for 2 h. Compared with the untreated wood, the lowest temperature resulted in the highest improvement of 271.1 and 218.2% in glucose and ethanol production yields, respectively. In addition to the pretreatment, Tween-20 as a no ...
... Sequential fractionation has been widely used to study the nature and dynamics of soil P. Residual P – the recalcitrant P fraction remaining after sequential extraction with alkali and acid reagents – often constitutes the majority of the soil P, yet its nature and bioavailability is poorly understood. The objective of this study was to isolate, quantify, and characterize residual P following Hedl ...
... The development of edible, environmentally friendly, mechanically strong and antimicrobial biopolymer films for active food packaging has gained considerable interest in recent years. The present work deals with the extraction and deacetylation of chitin nanofibers (ChNFs) from crab shells and their utilization as reinforcing and antimicrobial agents in carboxymethyl cellulose (CMC) films. ChNFs w ...
activated carbon; alkali treatment; byproducts; cation exchange; coagulants; disinfection; drinking water; iron; organic compounds; pH; risk; sludge; solubilization; turbidity; ultrafiltration; waste disposal; water quality; water treatment; water utilities; water utilization
Abstract:
... Coagulant recovery and reuse from waterworks sludge has the potential to significantly reduce waste disposal and chemicals usage for water treatment. Drinking water regulations demand purification of recovered coagulant before they can be safely reused, due to the risk of disinfection by-product precursors being recovered from waterworks sludge alongside coagulant metals. While several full-scale ...
... This investigation focused on the study of formation mechanism for highly mesoporous ZSM-5 under the introduction of tetrapropylammonium hydroxide (TPAOH) during NaOH treatment. Determined based on N₂ sorption, transmission electron spectroscopy, scanning electron spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, inductively coupled plasma atomic emission spectroscopy, and ²⁹Si ma ...
... BACKGROUND: Imperata cylindrica is being considered as a biomass candidate for bioethanol. This work aimed to evaluate a mild alkali pretreatment effect on the Imperata recalcitrant structure. Therefore, varied concentrations of NaOH (0, 7.5, 15, 20, and 25 g L⁻¹) were applied as treatments to Imperata at 105 °C for 10 min. RESULTS: Scanning electron microscopy, atomic force microscopy, and Fourie ...
... The aim of this work was to develop and optimize process parameters in ultrasound and sono‐assisted alkaline pretreatment of sugarcane bagasse prior to hydrolysis and bioethanol fermentation processes. Following the optimization of ultrasound time and temperature, the influence of concentration of NaOH solution was examined. The optimal condition in ultrasound pretreatment (35 kHz) was achieved at ...
acid treatment; actin; alkali treatment; chickens; humans; liver; myosin heavy chains; pH; polyacrylamide gel electrophoresis; protein composition; solubilization
Abstract:
... This study focused on the extraction efficiency and composition of proteins recovered from chicken liver after extraction by acid/alkaline solubilization (pH 2.0, 2.5, 3.0 and 3.5) and (pH 10.5, 11.0, 11.5 and 12.0) and precipitation in pH 5.5. The highest extracted protein yield was obtained at pH values of 2.0, 11.5 and 12.0 (77.9%, 78.4% and 79.3% respectively), and the lowest yield (58.5%) was ...