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You searched for: Subject "G-protein coupled receptors" Remove constraint Subject: "G-protein coupled receptors" Publication Year 2016 Remove constraint Publication Year: 2016 Subject humans Remove constraint Subject: humans Subject G-protein coupled receptors Remove constraint Subject: G-protein coupled receptors Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Structural modeling of G-protein coupled receptors: An overview on automatic web-servers

Author:
Busato, Mirko; Giorgetti, Alejandro
Source:
The international journal of biochemistry & cell biology 2016 v.77 pp. 264-274
ISSN:
1357-2725
Subject:
G-protein coupled receptors,   etc   ; crystallization; crystallography; databases; humans; models; prediction;     Show all 7 Subjects
Abstract:
... Despite the significant efforts and discoveries during the last few years in G protein-coupled receptor (GPCR) expression and crystallization, the receptors with known structures to date are limited only to a small fraction of human GPCRs. The lack of experimental three-dimensional structures of the receptors represents a strong limitation that hampers a deep understanding of their function. Compu ...
DOI:
10.1016/j.biocel.2016.04.004

2. A Comparison between the Photoactivation Kinetics of Human and Bovine Rhodopsins

Author:
Funatogawa, Chie; Szundi, Istvan; Kliger, David S.
Source:
Biochemistry 2016 v.55 no.50 pp. 7005-7013
ISSN:
1520-4995
Subject:
G-protein coupled receptors,   etc   ; cattle; humans; pH; photolysis; rhodopsin; schiff bases; spectroscopy; vision;     Show all 9 Subjects
Abstract:
... Rhodopsin is a G-protein-coupled receptor important for vertebrate vision under dim light conditions. Many studies of the activation mechanism of bovine rhodopsin have been conducted, but there have been relatively few investigations of the human protein. A recent study of the late photointermediates of bovine rhodopsin studies at 15 °C and pH 7.3, 8.0, and 8.7 revealed a rather complex activation ...
DOI:
10.1021/acs.biochem.6b00953

3. Human DP and EP2 prostanoid receptors take on distinct forms depending on the diverse binding of different ligands

Author:
Suganami, Akiko; Fujino, Hiromichi; Okura, Iori; Yanagisawa, Naoki; Sugiyama, Hajime; Regan, John W.; Tamura, Yutaka; Murayama, Toshihiko
Source:
The FEBS journal 2016 v.283 no.21 pp. 3931-3940
ISSN:
1742-464X
Subject:
G-protein coupled receptors,   etc   ; cyclic AMP; genes; humans; ligands; prostaglandins; signal transduction;     Show all 7 Subjects
Abstract:
... Human D‐type prostanoid (DP) and E‐type prostanoid 2 (EP2) receptors are G protein‐coupled receptors and are regarded as the most closely related receptors among prostanoid receptors because they are generated by tandem duplication. The DP receptor‐cognate ligand, prostaglandin D₂ (PGD₂) has the ability to activate not only DP receptors but also EP2 receptors. Likewise, the EP2 receptor‐cognate li ...
DOI:
10.1111/febs.13899

4. A negatively charged transmembrane aspartate residue controls activation of the relaxin-3 receptor RXFP3

Author:
Liu, Yu; Zhang, Lei; Shao, Xiao-Xia; Hu, Meng-Jun; Liu, Ya-Li; Xu, Zeng-Guang; Guo, Zhan-Yun
Source:
Archives of biochemistry and biophysics 2016 v.604 pp. 113-120
ISSN:
0003-9861
Subject:
G-protein coupled receptors,   etc   ; agonists; aspartic acid; food intake; humans; insulin; mutants; neuropeptides; relaxin; stress response;     Show all 10 Subjects
Abstract:
... Relaxin-3 is an insulin/relaxin superfamily neuropeptide involved in the regulation of food intake and stress response via activation of its cognate receptor RXFP3, an A-class G protein-coupled receptor (GPCR). In recent studies, a highly conserved ExxxD motif essential for binding of relaxin-3 has been identified at extracellular end of the second transmembrane domain (TMD2) of RXFP3. For most of ...
DOI:
10.1016/j.abb.2016.06.013

5. A novel cell-based duplex high-throughput screening assay combining fluorescent Ca2+ measurement with homogeneous time-resolved fluorescence technology

Author:
Kiss, László; Cselenyák, Attila; Varga, Ágnes; Visegrády, András
Source:
Analytical biochemistry 2016 v.507 pp. 33-39
ISSN:
0003-2697
Subject:
G-protein coupled receptors,   etc   ; calcium; cyclic AMP; fluorescence; fluorometry; genetic engineering; humans; ligands; screening; second messengers;     Show all 10 Subjects
Abstract:
... Cell-based assays for G-protein-coupled receptor (GPCR) activation applied in high-throughput screening (HTS) monitor various readouts for second messengers or intracellular effectors. Recently, our understanding of diverging signaling pathways downstream of receptor activation and the capability of small molecules to selectively modulate signaling routes has increased substantially, underlining t ...
DOI:
10.1016/j.ab.2016.05.009

6. Predicting G protein-coupled receptor downstream signaling by tissue expression

Author:
Hao, Yun; Tatonetti, Nicholas P.
Source:
Bioinformatics 2016 v.32 no.22 pp. 3435-3443
ISSN:
1460-2059
Subject:
G-protein coupled receptors,   etc   ; G-proteins; bioinformatics; cell lines; data collection; humans; prediction; protein-protein interactions; tissues;     Show all 9 Subjects
Abstract:
... Motivation: G protein-coupled receptors (GPCRs) are central to how cells respond to their environment and a major class of pharmacological targets. However, comprehensive knowledge of which pathways are activated and deactivated by these essential sensors is largely unknown. To better understand the mechanism of GPCR signaling system, we integrated five independent genome-wide expression datasets, ...
DOI:
10.1093/bioinformatics/btw510

7. Nuclear localization of Formyl-Peptide Receptor 2 in human cancer cells

Author:
Cattaneo, Fabio; Parisi, Melania; Fioretti, Tiziana; Sarnataro, Daniela; Esposito, Gabriella; Ammendola, Rosario
Source:
Archives of biochemistry and biophysics 2016 v.603 pp. 10-19
ISSN:
0003-9861
Subject:
G-protein coupled receptors,   etc   ; Western blotting; agonists; fluorescent antibody technique; humans; models; mutagenesis; neoplasm cells; neoplasms; nuclear localization signals; phosphorylation;     Show all 11 Subjects
Abstract:
... Current models of G protein-coupled receptors (GPCRs) signaling describe binding of external agonists to cell surface receptors which, in turn, trigger several biological responses. New paradigms indicate that GPCRs localize to and signal at the nucleus, thus regulating distinct signaling cascades. The formyl-peptide receptor FPR2 belongs to the GPCR super-family and is coupled to PTX-sensitive Gi ...
DOI:
10.1016/j.abb.2016.05.006

8. Application of the novel bioluminescent ligand–receptor binding assay to relaxin-RXFP1 system for interaction studies

Author:
Wu, Qing-Ping; Zhang, Lei; Shao, Xiao-Xia; Wang, Jia-Hui; Gao, Yu; Xu, Zeng-Guang; Liu, Ya-Li; Guo, Zhan-Yun
Source:
Amino acids 2016 v.48 no.4 pp. 1099-1107
ISSN:
0939-4451
Subject:
G-protein coupled receptors,   etc   ; crosslinking; screening; Komagataella pastoris; humans; antagonists; ligands; bioluminescence; peptide hormones; relaxin; agonists; binding capacity; cysteine;     Show all 13 Subjects
Abstract:
... Relaxin is a prototype of the relaxin family peptide hormones and plays important biological functions by binding and activating the G protein-coupled receptor RXFP1. To study their interactions, in the present work, we applied the newly developed bioluminescent ligand–receptor binding assay to the relaxin-RXFP1 system. First, a fully active easily labeled relaxin, in which three Lys residues of h ...
DOI:
10.1007/s00726-015-2146-3

9. The C-terminus of the B-chain of human insulin-like peptide 5 is critical for cognate RXFP4 receptor activity

Author:
Patil, Nitin A.; Bathgate, Ross A. D.; Kocan, Martina; Ang, Sheng Yu; Tailhades, Julien; Separovic, Frances; Summers, Roger; Grosse, Johannes; Hughes, Richard A.; Wade, John D.; Hossain, Mohammed Akhter
Source:
Amino acids 2016 v.48 no.4 pp. 987-992
ISSN:
0939-4451
Subject:
G-protein coupled receptors,   etc   ; acids; amides; anorexia; colon; disulfide bonds; drugs; electrostatic interactions; food intake; humans; obesity; peptides; relaxin;     Show all 13 Subjects
Abstract:
... Insulin-like peptide 5 (INSL5) is an orexigenic peptide hormone belonging to the relaxin family of peptides. It is expressed primarily in the L-cells of the colon and has a postulated key role in regulating food intake. Its G protein-coupled receptor, RXFP4, is a potential drug target for treating obesity and anorexia. We studied the effect of modification of the C-terminus of the A and B-chains o ...
DOI:
10.1007/s00726-015-2144-5

10. β-arrestins regulate gonadotropin receptor-mediated cell proliferation and apoptosis by controlling different FSHR or LHCGR intracellular signaling in the hGL5 cell line

Author:
Casarini, Livio; Reiter, Eric; Simoni, Manuela
Source:
Molecular and Cellular Endocrinology 2016 v.437 pp. 11-21
ISSN:
0303-7207
Subject:
G-protein coupled receptors,   etc   ; apoptosis; cAMP-dependent protein kinase; cell proliferation; cyclic AMP; death; follicle-stimulating hormone; gonadotropin receptors; humans; ligands; luteinizing hormone; phosphorylation; progesterone;     Show all 13 Subjects
Abstract:
... Gonadotropin signaling classically involves proliferative, steroidogenic and apoptotic stimuli. In this study, we used the human granulosa cell line hGL5 to demonstrate how follicle-stimulating hormone (FSH) and luteinizing hormone (LH) differently control proliferative or apoptotic signals, revealing novel intrinsic properties of their receptors (FSHR, LHCGR). We found that, in this tumor-like ce ...
DOI:
10.1016/j.mce.2016.08.005

11. Apelin binding to human APJ receptor leads to biased signaling Proteins and proteomics

Author:
Kumar, Prashant; Ashokan, Anisha; Aradhyam, Gopala Krishna
Source:
Biochimica et biophysica acta 2016 v.1864 no.12 pp. 1748-1756
ISSN:
1570-9639
Subject:
G-protein coupled receptors,   etc   ; angiogenesis; brain; calcium; cardiac output; heart; homeostasis; humans; kidneys; ligands; muscles; mutants; pathophysiology; phosphorylation; rhodopsin; sequence alignment;     Show all 16 Subjects
Abstract:
... Human APJ receptor (APJR), a rhodopsin family G-Protein Coupled Receptor (GPCR), activated by isoforms of peptide ligand apelin causing potent inotropic effect, is involved in cardiac function, angiogenesis and maintenance of fluid homeostasis. APJR is expressed in various organs e.g., heart, brain, kidney, muscles, etc. Hence, problems in APJR signaling lead to severe dysregulation in the pathoph ...
DOI:
10.1016/j.bbapap.2016.09.012

12. Estrogen receptor signaling in the ferutinin-induced osteoblastic differentiation of human amniotic fluid stem cells

Author:
Zavatti, M.; Guida, M.; Maraldi, T.; Beretti, F.; Bertoni, L.; La Sala, G.B.; De Pol, A.
Source:
Life sciences 2016 v.164 pp. 15-22
ISSN:
0024-3205
Subject:
G-protein coupled receptors,   etc   ; amniotic fluid; estrogen receptors; estrogenic properties; fluorescent antibody technique; humans; mitogen-activated protein kinase; phosphatidylinositol 3-kinase; sesquiterpenoids; signal transduction; stem cells;     Show all 11 Subjects
Abstract:
... Ferutinin is a diaucane sesquiterpene with a high estrogenic activity. Since ferutinin is able to enhance osteoblastic differentiation of human amniotic fluid stem cells (hAFSCs), the aim of this study was to evaluate the role of the estrogen receptors α (ERα) and G-protein coupled receptor 30 (GPR30) in ferutinin-mediated osteoblastic differentiation. Moreover, it was investigated if MEK/ERK and ...
DOI:
10.1016/j.lfs.2016.09.005

13. Thermostabilization of the Human Endothelin Type B Receptor

Author:
Okuta, Akiko; Tani, Kazutoshi; Nishimura, Shoko; Fujiyoshi, Yoshinori; Doi, Tomoko
Source:
Journal of Molecular Biology 2016 v.428 pp. 2265-2274
ISSN:
0022-2836
Subject:
G-protein coupled receptors,   etc   ; Escherichia coli; alanine; endothelins; endothelium; heat treatment; homeostasis; humans; medicinal properties; melting point; mutagenesis; mutants; point mutation; thermal stability;     Show all 14 Subjects
Abstract:
... The peptide hormone endothelin, produced by the vascular endothelium, is involved in several physiological functions, including maintenance of vascular tone and humoral homeostasis. Endothelin transmits signals through the endothelin receptor, a G-protein-coupled receptor. Structural studies of the endothelin type B receptor (ETBR) have been unsuccessful due to its structural flexibility and insta ...
DOI:
10.1016/j.jmb.2016.03.024

14. Investigation of the Binding Interaction of Fatty Acids with Human G Protein-Coupled Receptor 40 Using a Site-Specific Fluorescence Probe by Flow Cytometry

Author:
Ren, Xiao-Min; Cao, Lin-Ying; Zhang, Jing; Qin, Wei-Ping; Yang, Yu; Wan, Bin; Guo, Liang-Hong
Source:
Biochemistry 2016 v.55 no.13 pp. 1989-1996
ISSN:
1520-4995
Subject:
G-protein coupled receptors,   etc   ; binding capacity; carbon; flow cytometry; fluorescence; free fatty acids; humans; insulin secretion; kidneys; ligands; molecular models; myristic acid;     Show all 12 Subjects
Abstract:
... Human G protein-coupled receptor 40 (hGPR40), with medium- and long-chain free fatty acids (FFAs) as its natural ligands, plays an important role in the enhancement of glucose-dependent insulin secretion. To date, information about the direct binding of FFAs to hGPR40 is very limited, and how carbon-chain length affects the activities of FFAs on hGPR40 is not yet understood. In this study, a fluor ...
DOI:
10.1021/acs.biochem.6b00079

15. mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts

Author:
Caruso, Vanni; Sreedharan, Smitha; Carlini, Valeria P.; Jacobsson, Josefin A.; Haitina, Tatjana; Hammer, Joanna; Stephansson, Olga; Crona, Filip; Sommer, Wolfgang H.; Risérus, Ulf; Lannfelt, Lars; Marcus, Claude; Heilig, Markus; de Barioglio, Susana R.; Fredriksson, Robert; Schiöth, Helgi B.
Source:
Gene 2016 v.581 no.2 pp. 139-145
ISSN:
0378-1119
Subject:
G-protein coupled receptors,   etc   ; amygdala; blood pressure; energy; food intake; gene expression; genes; genetic variation; glucose; hippocampus; homeostasis; humans; hypothalamus; in situ hybridization; messenger RNA; mice; neurons; physiological state; rats; rhodopsin; tissues; Sweden;     Show all 22 Subjects
Abstract:
... G protein–coupled receptors (GPCRs) are a class of integral membrane proteins mediating intercellular interactions of fundamental physiological importance for survival including regulation of food intake, blood pressure, and hormonal sensing signaling, among other roles. Homeostatic alterations in the physiological status of GPCRs are often associated with underlying causes of disease, and to date ...
DOI:
10.1016/j.gene.2016.01.044

16. Trans‐11 vaccenic acid improves insulin secretion in models of type 2 diabetes in vivo and in vitro

Author:
Wang, Xiaofeng; Gupta, Joel; Kerslake, Matthew; Rayat, Gina; Proctor, Spencer D.; Chan, Catherine B.
Source:
Molecular nutrition & food research 2016 v.60 no.4 pp. 846-857
ISSN:
1613-4125
Subject:
G-protein coupled receptors,   etc   ; c-peptide; dairy products; dietary supplements; food availability; gene expression; glucose; humans; insulin secretion; islets of Langerhans; meat; messenger RNA; models; noninsulin-dependent diabetes mellitus; rats; risk; ruminants; trans fatty acids; vaccenic acid;     Show all 19 Subjects
Abstract:
... SCOPE: Trans‐11 vaccenic acid (VA) is a fatty acid produced by ruminants entering the human food supply through meat and dairy products, which appears not to have the health risks associated with industrially produced trans‐fatty acids. In this study, we investigated the effect of VA on insulin secretion in vivo in rats and in vitro in human and rat islets after diabetogenic insult. METHODS AND RE ...
DOI:
10.1002/mnfr.201500783

17. The effects on weight loss and gene expression in adipose and hepatic tissues of very-low carbohydrate and low-fat isoenergetic diets in diet-induced obese mice

Author:
Yamazaki, Tomomi; Okawa, Sumire; Takahashi, Mayumi
Source:
Nutrition & metabolism 2016 v.13 no.1 pp. 78
ISSN:
1743-7075
Subject:
G-protein coupled receptors,   etc   ; animal disease models; blood serum; brown adipose tissue; carbohydrate intake; carbohydrates; energy expenditure; energy intake; energy metabolism; fatty acids; fibroblast growth factors; gene expression; high fat diet; humans; iodide peroxidase; ketone bodies; lipogenesis; liver; low carbohydrate diet; low fat diet; macrophages; males; messenger RNA; mice; obesity; weight loss; white adipose tissue;     Show all 27 Subjects
Abstract:
... BACKGROUND: Obesity is caused by excessive fat or carbohydrate intake. The improvement of obesity is an important issue, especially in Western societies. Both low-carbohydrate diet (LCD) and low-fat diet (LFD) are used to achieve weight loss in humans. To clarify the mechanisms underlying LCD-induced weight loss, especially in early stage, we compared the gene expression in liver, white adipose ti ...
DOI:
10.1186/s12986-016-0139-1

18. The effect of G protein-coupled receptor kinase 2 (GRK2) on lactation and on proliferation of mammary epithelial cells from dairy cows

Author:
Hou, Xiaoming; Hu, Hongliu; Lin, Ye; Qu, Bo; Gao, Xuejun; Li, Qingzhang
Source:
Journal of dairy science 2016 v.99 no.7 pp. 5828-5836
ISSN:
0022-0302
Subject:
G-protein coupled receptors,   etc   ; epithelial cells; milk; genes; mitogen-activated protein kinase; dairy cows; neoplasms; microfilaments; gene expression; dietary nutrient sources; mammary glands; gene expression regulation; animal tissues; humans; milk synthesis; insulin; milk fat; non-specific serine/threonine protein kinase; milk proteins; lactation; biochemical pathways;     Show all 21 Subjects
Abstract:
... Milk protein is an important component of milk and a nutritional source for human consumption. To better understand the molecular events underlying synthesis of milk proteins, the global gene expression patterns in mammary glands of dairy cow with high-quality milk (>3% milk protein; >3.5% milk fat) and low-quality milk (<3% milk protein; <3.5% milk fat) were examined via digital gene expression s ...
DOI:
10.3168/jds.2015-10560

19. Characterization of the adipokinetic hormone receptor of the anautogenous flesh fly, Sarcophaga crassipalpis

Author:
Bil, Magdalena; Timmermans, Iris; Verlinden, Heleen; Huybrechts, Roger
Source:
Journal of insect physiology 2016 v.89 pp. 52-59
ISSN:
0022-1910
Subject:
G-protein coupled receptors,   etc   ; Periplaneta americana; Phormia regina; Protophormia terraenovae; Pyrrhocoris apterus; Sarcophaga crassipalpis; Tribolium castaneum; adipokinetic hormone; adipokinetic hormone receptors; brain; digestion; energy; fat body; females; foregut; gonadotropin-releasing hormone; hindgut; humans; hypertrehalosemic hormone; insect physiology; insects; ligands; median effective concentration; neuropeptides; vitellogenesis; yolk proteins;     Show all 26 Subjects
Abstract:
... Adipokinetic hormone (AKH) is an insect neuropeptide mainly involved in fat body energy mobilization. In flies (Phormia regina, Sarcophaga crassipalpis), bugs (Pyrrhocoris apterus) and cockroaches (Periplaneta americana) AKH was also demonstrated to be involved in the regulation of digestion. This makes AKH an important peptide for anautogenous female flies that need to feed on a supplementary pro ...
DOI:
10.1016/j.jinsphys.2016.04.001