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- Cheng, Fei; Hou, Lin; Woeste, Keith; Shang, Zhengchun; Peng, Xiaobang; Zhao, Peng; Zhang, Shuoxin
- Brazilian journal of microbiology 2016 v.47 no.4 pp. 817-827
- calcium chloride, etc ; DNA; calcium; community structure; flocculation; forest soils; fungal communities; fungi; glass; humic substances; internal transcribed spacers; octoxynol; pH; polymerase chain reaction; polyvinylpyrrolidone; restriction fragment length polymorphism; skim milk; sodium chloride; soil biota; soil pollution; soil sampling; transcription (genetics); Show all 22 Subjects
- ... Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbiota, ...
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