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- Ki, Mi-Ran; Nguyen, Thi Khoa My; Kim, Sung Ho; Kwon, Inchan; Pack, Seung Pil
- Process biochemistry 2016 v.51 no.9 pp. 1222-1229
- recombinant fusion proteins, etc ; Dunaliella; amino acids; calcite; calcium chloride; carbon dioxide; carbon sequestration; carbonate dehydratase; catalytic activity; enzyme activity; genes; solubility; thermal stability; Show all 13 Subjects
- ... An internally duplicated α-type carbonic anhydrase (CA) from Dunaliella species (Dsp-CA) has been expressed as single-domain derivatives, N- and C- half domains. Although both derivatives have structures similar to that of a known CA (PDB ID: 1y7w), only the C-half domain (Dsp-CA-c) exhibited enzymatic activity, albeit with low solubility. In order to increase the solubility of Dsp-CA-c, its proxi ...
- Jiang, Ting; Kang, Chao; Yu, Xiao-Wei; Xu, Yan
- Journal of molecular catalysis 2016 v.125 pp. 81-87
- recombinant fusion proteins, etc ; Aspergillus oryzae; Pichia pastoris; Streptomyces violaceoruber; binding proteins; catalytic activity; enzyme activity; genes; maltose; pH; phospholipase A2; protein content; protein synthesis; Show all 13 Subjects
- ... In our previous studies, a prolyl endopeptidase (PEP) gene from Aspergillus oryzae (MOH) was cloned and expressed in Pichia pastoris; however, the recombinant protein expression level of MOH was very low. In the present study, the PEP expression level was successfully improved by constructing fusion expression proteins with four fusion partners, namely, Streptomyces violaceoruber Phospholipase A2 ...
- Ribeiro, Lucas Ferreira; Tullman, Jennifer; Nicholes, Nathan; Silva, Sérgio Ruschi Bergamachi; Vieira, Davi Serradella; Ostermeier, Marc; Ward, Richard John
- Biotechnology for biofuels 2016 v.9 no.1 pp. 119
- recombinant fusion proteins, etc ; Bacillus subtilis; Escherichia coli; active sites; asparagine; binding proteins; catalytic activity; clones; deoxyribonuclease I; engineering; fluorescence; genes; glycosides; lignocellulose; molecular dynamics; plasmids; protein-protein interactions; saccharification; screening; staining; xylan; xylanases; xylose; Show all 23 Subjects
- ... BACKGROUND: Saccharification of lignocellulosic material by xylanases and other glycoside hydrolases is generally conducted at high concentrations of the final reaction products, which frequently inhibit the enzymes used in the saccharification process. Using a random nonhomologous recombination strategy, we have fused the GH11 xylanase from Bacillus subtilis (XynA) with the xylose binding protein ...
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