Jump to Main Content
- Moore, Stephanie
M.; Hess, Stephanie M.; Jorgenson, James W.
- Journal of Proteome Research 2016 v.15 no.4 pp. 1243-1252
- sonication, etc ; Saccharomyces cerevisiae; deoxycholic acid; detergents; digestion; membrane proteins; proteome; proteomics; solubilization; surfactants; yeasts; Show all 11 Subjects
- ... The importance of membrane proteins in biological systems is indisputable; however, their amphipathic nature makes them difficult to analyze. In this study, the most popular techniques for extraction, enrichment, solubilization, and digestion are compared, resulting in an overall improved workflow for the insoluble portion of Saccharomyces cerevisiae cell lysate. Yeast cells were successfully lyse ...
- PubMed Central: