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1. Activation of G protein-coupled receptor 30 by thiodiphenol promotes proliferation of estrogen receptor α-positive breast cancer cells

Author:
Lei, Bingli; Peng, Wei; Xu, Gang; Wu, Minghong; Wen, Yu; Xu, Jie; Yu, Zhiqiang; Wang, Yipei
Source:
Chemosphere 2016
ISSN:
0045-6535
Subject:
cell viability,   etc   ; G-protein coupled receptors; bisphenol A; breast neoplasms; calcium; cell proliferation; estrogen receptors; estrogenic properties; neoplasm cells; phosphorylation; reactive oxygen species; signal transduction;     Show all 12 Subjects
Abstract:
... Many studies have been shown that environmental estrogen bisphenol A (BPA) can activate nuclear receptor (estrogen receptor alpha, ERα) or membrane receptor (G-protein-coupled receptor, GPR30) in breast cancer cells and exerts genomic or nongenomic actions inducing cell proliferation. 4,4′-thiodiphenol (TDP) as one of BPA derivatives exhibits more potent estrogenic activity than BPA does. However, ...
DOI:
10.1016/j.chemosphere.2016.11.066

2. Altering the central carbon metabolism of HEK293 cells: Impact on recombinant glycoprotein quality

Author:
Karengera, Eric; Robotham, Anna; Kelly, John; Durocher, Yves; De Crescenzo, Gregory; Henry, Olivier
Source:
Journal of biotechnology 2016
ISSN:
0168-1656
Subject:
viability,   etc   ; byproducts; carbon; densitometry; gene overexpression; genes; glycoproteins; glycosylation; humans; interferon-alpha; liquid chromatography; mass spectrometry; metabolic engineering; metabolism; metabolites; polyacrylamide gel electrophoresis; product quality; proteolysis; recombinant proteins;     Show all 19 Subjects
Abstract:
... The accumulation of metabolic by-products remains a critical challenge in the development of mammalian cells culture processes as it impacts cellular growth, productivity and product quality. Although the overexpression of the PYC2 gene was shown to significantly improve the nutrient metabolism efficiency of mammalian cells, its impact on recombinant protein quality has not been investigated yet. ...
DOI:
10.1016/j.jbiotec.2016.12.003

3. Antioxidant enzyme activities as biomarkers of fluvial biofilm to ZnO NPs ecotoxicity and the Integrated Biomarker Responses (IBR) assessment

Author:
Hou, Jun; You, Guoxiang; Xu, Yi; Wang, Chao; Wang, Peifang; Miao, Lingzhan; Dai, Shanshan; Lv, Bowen; Yang, Yangyang
Source:
Ecotoxicology and environmental safety 2016
ISSN:
0147-6513
Subject:
viability,   etc   ; adverse effects; bacteria; biofilm; biomarkers; catalase; ecotoxicology; environmental impact; enzyme activity; exposure duration; glutathione peroxidase; glutathione-disulfide reductase; nanoparticles; reactive oxygen species; scanning electron microscopy; superoxide dismutase; toxicity; zinc oxide;     Show all 18 Subjects
Abstract:
... The presence of ZnO nanoparticles (ZnO NPs) in natural waters has raised concerns about their environmental impacts, but the potential influences of ZnO NPs on fluvial biofilm have not been reported. In this study, the utility of antioxidant enzyme activities (AEA) as biomarkers of fluvial biofilm to ZnO NPs toxicity and a method that combines AEA into an index of “Integrated Biomarker Responses ( ...
DOI:
10.1016/j.ecoenv.2016.06.014

4. Applicability of the photogrammetry technique to determine the volume and the bulk density of small soil aggregates

Author:
Moret-Fernández, D.; Latorre, B.; Peña, C.; González-Cebollada, C.; López, M. V.
Source:
Soil research 2016 v.54 no.3 pp. 354-359
ISSN:
1838-675X
Subject:
viability,   etc   ; bulk density; cameras; conservation tillage; disturbed soils; photogrammetry; photographs; soil aggregates;     Show all 8 Subjects
Abstract:
... Aggregate density (ρ) is defined as the relationship between the mass and the volume occupied by an aggregate. Previous studies have characterised ρ on large to medium-sized soil aggregates (>4mm diameter); however, little information is available for smaller aggregates (<4mm). The objective of this study was to test the viability of the photogrammetry (PHM) technique to determine the volume and s ...
DOI:
10.1071/SR15163

5. Arsenic and 17-β-estradiol bind to each other and neutralize each other’s signaling effects

Author:
Kumar, Sukhdeep; Mukherjee, Tapan K.; Guptasarma, Purnananda
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
cell viability,   etc   ; absorption; arsenic; breast neoplasms; carbon; elderly; men; menarche; menopause; morbidity; neutralization; nuclear magnetic resonance spectroscopy; spermatozoa; women;     Show all 14 Subjects
Abstract:
... We report that arsenic trioxide (ATO) and 17-beta-estradiol (E2) abolish each other’s independent cell signaling effects in respect of cell survival and proliferation/migration of breast cancer (MCF-7) cells. The possibility that this is due to binding of ATO to E2 was confirmed through difference absorption spectroscopy, chromatography-coupled voltammometry and 1-D 1H and 13C NMR spectroscopy. Bi ...
DOI:
10.1016/j.bbrc.2016.06.087

6. A simple tube adapter to expedite and automate thawing of viably frozen cells

Author:
Beddall, Margaret; Chattopadhyay, Pratip K.; Kao, Shing-Fen; Foulds, Kathy; Roederer, Mario
Source:
Journal of Immunological Methods 2016
ISSN:
0022-1759
Subject:
cell viability,   etc   ; T-lymphocytes; biomedical research; cell culture; cryopreservation; culture media; labor; models; thawing; toxicity;     Show all 10 Subjects
Abstract:
... Although cryopreserved cell specimens are used throughout biomedical research, the process for thawing samples is labor-intensive and prone to error. Here we describe a small laboratory device that couples an uncapped vial of frozen cells to a conical tube containing warm cell culture media. The entire complex is loaded directly into a centrifuge; within 5min, cells are thawed and diluted out of t ...
DOI:
10.1016/j.jim.2016.08.009

7. Autophagy is an important metabolic pathway to determine leukemia cell survival following suppression of the glycolytic pathway

Author:
Kawaguchi, Moemi; Aoki, Shigeki; Hirao, Takuya; Morita, Michie; Ito, Kousei
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
cell viability,   etc   ; adenosine triphosphate; autophagy; biochemical pathways; energy; glycolysis; mitochondria; neoplasm cells; neoplasms; oxygen; therapeutics;     Show all 11 Subjects
Abstract:
... Most cancer cells predominantly produce energy by glycolysis, even in the presence of adequate oxygen. Therefore, inhibition of glycolysis is a promising cancer treatment target. Recently, it has been recognized that to conduct thorough treatment of cancer, comprehensive understanding of cancer metabolism is essential, not only focusing on glycolysis. Here, we investigated the supporting mechanism ...
DOI:
10.1016/j.bbrc.2016.04.098

8. Berberine attenuates Aβ25−35-induced apoptosis in primary cultured hippocampal neurons

Author:
Huang, Min; Liang, Yubin; Liu, Qiong; Chang, Xin; Guo, Yi
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
cell viability,   etc   ; Alzheimer disease; apoptosis; berberine; caspases; cytochrome c; cytotoxicity; neurodegenerative diseases; neurons; neuroprotective effect; rhizomes; therapeutics;     Show all 12 Subjects
Abstract:
... Alzheimer's disease (AD) is the most common age-related neurodegenerative disease and is characterized by the production of β-amyloid (Aβ) proteins and progressive loss neurons. Berberine, an isoquinoline alkaloid isolated and extracted from Coptidis and rhizome and Cortex phellodendri, has a wide range of pharmacological effects. Here, we examined the neuroprotective effects of Berberine against ...
DOI:
10.1016/j.bbrc.2016.12.166

9. Beta-adrenergic signaling affect osteoclastogenesis via osteocytic MLO-Y4 cells' RANKL production

Author:
Yao, Qianqian; Liang, Hengxing; Huang, Bo; Xiang, Lin; Wang, Tianlu; Xiong, Yi; Yang, Bo; Guo, Yanjun; Gong, Ping
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
cell viability,   etc   ; antagonists; beta-1 adrenergic receptors; coculture; enzymes; genes; osteoclasts; sympathetic nervous system; therapeutics;     Show all 9 Subjects
Abstract:
... The sympathetic nervous system play a pivotal role in bone remodeling through β-adrenoceptor (β-AR). However, it is not well documented whether the β-adrenoceptor pathway has the potential to influence osteocytes. In this study, cell viability, the expression of β-AR subtypes, enzymes of catecholamine synthesis or degradation, bone-related gene and protein in osteocytic MLO-Y4 cells were investiga ...
DOI:
10.1016/j.bbrc.2016.11.011

10. Brazilian Cerrado soil reveals an untapped microbial potential for unpretreated polyethylene biodegradation

Author:
Peixoto, Julianna; Silva, Luciano P.; Kruger, Ricardo H.
Source:
Journal of hazardous materials 2016
ISSN:
0304-3894
Subject:
viability,   etc   ; Comamonas; Delftia; Fourier transform infrared spectroscopy; Raman spectroscopy; Stenotrophomonas; atomic force microscopy; bacteria; biodegradation; carbon; cerrado; cerrado soils; decontamination; image analysis; metabolism; molecular weight; oxidation; polyethylene; scanning electron microscopy; waste management;     Show all 20 Subjects
Abstract:
... Discarded PE-based products pose a social and environmental threat because of their recalcitrance to degradation, a consequence of the unique set of PE’s physicochemical properties. In this study we isolated nine novel PE-degrading bacteria from plastic debris found in soil of the savanna-like Brazilian Cerrado. These bacterial strains from the genera Comamonas, Delftia, and Stenotrophomonas showe ...
DOI:
10.1016/j.jhazmat.2016.11.037

11. Cell survival of glioblastoma grown in medium containing hydrogen peroxide and/or nitrite, or in plasma-activated medium

Author:
Kurake, Naoyuki; Tanaka, Hiromasa; Ishikawa, Kenji; Kondo, Takashi; Sekine, Makoto; Nakamura, Kae; Kajiyama, Hiroaki; Kikkawa, Fumitaka; Mizuno, Masaaki; Hori, Masaru
Source:
Archives of biochemistry and biophysics 2016
ISSN:
0003-9861
Subject:
cell viability,   etc   ; air; ambient temperature; antineoplastic activity; apoptosis; argon (noble gases); atmospheric pressure; culture media; electrons; epithelial cells; hydrogen peroxide; mammary glands; neoplasm cells; nitrites;     Show all 14 Subjects
Abstract:
... Non-equilibrium atmospheric pressure plasmas generate a high electron density (on the order of 1016 electrons per cm−3) using Ar gas. Culture medium in air at room temperature was plasma-irradiated for several hundred seconds. Tens of micromolar hydrogen peroxide (H2O2) and millimolar levels of nitrous ion (NO2−) were detected in the plasma-irradiated culture medium (plasma activated medium; PAM) ...
DOI:
10.1016/j.abb.2016.01.011

12. Changes in Rates of Capture and Demographics of Myotis septentrionalis (Northern Long-eared Bat) in Western Virginia before and after Onset of White-nose Syndrome

Author:
Reynolds, Richard J.; Powers, Karen E.; Orndorff, Wil; Ford, W. Mark; Hobson, Christopher S.
Source:
Northeastern naturalist 2016 v.23 no.2 pp. 195-204
ISSN:
1092-6194
Subject:
viability,   etc   ; Myotis septentrionalis; demographic statistics; juveniles; lactating females; lactation; summer; survival rate; swarming; white-nose syndrome; Virginia;     Show all 11 Subjects
Abstract:
... Documenting the impacts of white-nose syndrome (WNS) on demographic patterns, such as annual survivorship and recruitment, is important to understanding the extirpation or possible stabilization and recovery of species over time. To document demographic impacts of WNS on Myotis septentrionalis (Northern Long-eared Bat), we mistnetted at sites in western Virginia where Northern Long-eared Bats were ...
DOI:
10.1656/045.023.0201

13. Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm

Author:
Mosca, Fabio; Madeddu, Manuela; Sayed, Ahmad Abdel; Zaniboni, Luisa; Iaffaldano, Nicolaia; Cerolini, Silvia
Source:
Cryobiology 2016
ISSN:
0011-2240
Subject:
viability,   etc   ; adults; cryopreservation; cryoprotectants; lakes; nitrogen; roosters; semen; spermatozoa; sucrose; synergism; thawing; trehalose; vapors;     Show all 14 Subjects
Abstract:
... The aim of this study was to assess the combined effect of dimethylacetamide (DMA) and two non-permeating cryoprotective agents, trehalose and sucrose, on the quality of post-thaw chicken semen. Adult Lohmann roosters (n = 27) were used. Semen was processed according to the following treatments: Lake pre-freezing extender +6% DMA (LPF, control treatment), LPF + 0.1 M trehalose (LPF-T treatment), L ...
DOI:
10.1016/j.cryobiol.2016.10.001

14. Comparative efficacies of six different media for cryopreservation of immature buffalo (Bubalus bubalis) calf testis

Author:
Devi, Lalitha; Makala, Himesh; Pothana, Lavanya; Nirmalkar, Khemlal; Goel, Sandeep
Source:
Reproduction, fertility, and development 2016 v.28 no.7 pp. 872-885
ISSN:
1031-3613
Subject:
cell viability,   etc   ; DNA damage; Leydig cells; RNA-binding proteins; actin; apoptosis; buffaloes; calves; cryopreservation; cytochrome P-450; dairies; dimethyl sulfoxide; fetal bovine serum; freezing; germplasm conservation; males; mortality; sexual maturity; signal transduction; testosterone; tissues; transcription factors; vimentin;     Show all 23 Subjects
Abstract:
... Buffalo calves have a high mortality rate (~80%) in commercial dairies and testis cryopreservation can provide a feasible option for the preservation of germplasm from immature males that die before attaining sexual maturity. The aim of the present study was to evaluate combinations of 10 or 20% dimethylsulfoxide (DMSO) with 0, 20 or 80% fetal bovine serum (FBS) for cryopreservation of immature bu ...
DOI:
10.1071/RD14171

15. Compensating for a stressful start: maternal corticosterone, offspring survival, and size at fledging in the Zebra Finch, Taeniopygia guttata

Author:
Khan, Nicola; Peters, Richard A.; Robert, Kylie
Source:
Emu 2016 v.116 no.3 pp. 262-272
ISSN:
0158-4197
Subject:
viability,   etc   ; Taeniopygia guttata; adults; body condition; chicks; compensatory growth; corticosterone; eggs; females; fledglings; hatching; males; maternal effect; maternal nutrition; phenotype; progeny; reproductive performance; sex ratio;     Show all 18 Subjects
Abstract:
... All organisms are exposed to a variety of physical, environmental and psychological challenges throughout their lives. We administered repeated oral doses of the stress hormone corticosterone (CORT) to ovulating Zebra Finches (Taeniopygia guttata) in order to mimic a disruptive environment, and investigated the effect on their reproductive output as well as outcomes for their offspring. Although f ...
DOI:
10.1071/MU15095

16. Continuous treatment of non-sterile hospital wastewater by Trametes versicolor: How to increase fungal viability by means of operational strategies and pretreatments.

Author:
Mir-Tutusaus, J.A.; Sarrà, M.; Caminal, G.
Source:
Journal of hazardous materials 2016
ISSN:
0304-3894
Subject:
viability,   etc   ; Coriolus versicolor; active ingredients; bacteria; biomass; effluents; fungi; hospitals; ibuprofen; ketoprofen; laccase; ultraviolet radiation; wastewater;     Show all 13 Subjects
Abstract:
... Hospital wastewaters have a high load of pharmaceutical active compounds (PhACs). Fungal treatments could be appropriate for source treatment of such effluents but the transition to non-sterile conditions proved to be difficult due to competition with indigenous microorganisms, resulting in very short-duration operations. In this article, coagulation-flocculation and UV-radiation processes were st ...
DOI:
10.1016/j.jhazmat.2016.07.036

17. Cryobiological parameters of multipotent stromal cells obtained from different sources

Author:
Lauterboeck, L.; Wolkers, W.F.; Glasmacher, B.
Source:
Cryobiology 2016
ISSN:
0011-2240
Subject:
viability,   etc   ; Callithrix jacchus; activation energy; amnion; bone marrow; cell differentiation; cooling; cryopreservation; dimethyl sulfoxide; ice; medicine; membrane permeability; monkeys; stem cells; stromal cells; temperature;     Show all 16 Subjects
Abstract:
... Stem cells are important for regenerative medicine mainly due to their multilineage differentiation capacity. However, the cells rapidly loose this capability during culturing. Cryopreservation preserves the differentiation potential of the cells, until they are needed. In this study, specific cell properties of multipotent stromal cells (MSCs), from the common marmoset monkey Callithrix jacchus M ...
DOI:
10.1016/j.cryobiol.2016.11.009

18. Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15

Author:
Szychowski, Konrad A.; Binduga, Urszula E.; Rybczyńska-Tkaczyk, Kamila; Leja, Marcin L.; Gmiński, Jan
Source:
Saudi Journal of Biological Sciences 2016
ISSN:
1319-562X
Subject:
viability,   etc   ; Allium sativum; apoptosis; caspase-3; cultivars; cytotoxicity; garlic; humans; lactate dehydrogenase; reactive oxygen species;     Show all 10 Subjects
Abstract:
... Garlic (Allium sativum L., Alliaceae) has acquired a reputation as a therapeutic agent and herbal remedy to prevent and treat several pathologies. The aim of the present study was to determine the effect of two Allium sativum L. cultivars, Harnaś and Morado, on reactive oxygen species (ROS) production, viability and apoptotic process in human squamous carcinoma cell line SCC-15. The experiments we ...
DOI:
10.1016/j.sjbs.2016.10.005

19. 57 developmental characteristics of later-stage porcine embryos produced in vivo or in vitro

Author:
Callesen, H.; Holm, P.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 158-159
ISSN:
1031-3613
Subject:
cell viability,   etc   ; blastocyst; cell cycle; early development; embryogenesis; hatching; in vitro culture; in vitro studies; insemination; morula; oocytes; sows; zona pellucida; zygote;     Show all 14 Subjects
Abstract:
... Kinetics and morphological characteristics during pre-implantation embryo development are well established in most mammals. In porcine, such studies are few because of limited use of in vitro culture, but this has changed in recent years due to increasing use of in vitro production and cloning. Therefore, additional characterisation of especially later stage porcine embryos is needed. Here we stud ...
DOI:
10.1071/RDv28n2Ab56

20. 41 developmental potential of buffalo oocytes vitrified at the germinal vesicle stage: effects of different cryoprotectant combinations and cryodevices

Author:
El-Shalofy, A. S.; Moawad, A. R.; Darwish, G. M.; Ismail, S. T.; Badawy, A. B.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 150
ISSN:
1031-3613
Subject:
viability,   etc   ; blastocyst; buffaloes; cattle; cryopreservation; cryoprotectants; dimethyl sulfoxide; embryo culture; embryogenesis; ethylene glycol; females; germinal vesicle; glycerol; in vitro fertilization; slaughter; vitrification;     Show all 16 Subjects
Abstract:
... The cryopreservation of immature oocytes would generate a readily available, nonseasonal source of female gametes for both research and reproduction. In domestic animals, the most promising results in the field of oocyte cryopreservation have been reported in cattle, and a few experiments have been conducted on buffalo. The aim of the present study was to compare the effects of different cryoprote ...
DOI:
10.1071/RDv28n2Ab40

21. Differential genotypic effects of sexual trait size on offspring mating success and viability

Author:
Polak, Michal; Fanson, Kerry V.; Taylor, Phillip W.; Yap, Sarsha
Source:
Behavioral ecology 2016 v.27 no.2 pp. 444-451
ISSN:
1465-7279
Subject:
viability,   etc   ; Drosophila; females; males; progeny; reproductive success; sexual selection;     Show all 7 Subjects
Abstract:
... Sexual ornaments may signal males’ ability to produce superior offspring. In a study of Drosophila bipectinata, we showed that females mating with the most ornamented males produce offspring with higher survival and mating success. However, we also show that the mechanisms underlying effects on offspring survival and mating success are likely to be different. Knowledge of the way indirect benefits ...
DOI:
10.1093/beheco/arv174

22. Disruption of Serinc1, which facilitates serine-derived lipid synthesis, fails to alter macrophage function, lymphocyte proliferation or autoimmune disease susceptibility

Author:
Chu, Edward P.F.; Elso, Colleen M.; Pollock, Abigail H.; Alsayb, May A.; Mackin, Leanne; Thomas, Helen E.; Kay, Thomas W.H.; Silveira, Pablo A.; Mansell, Ashley S.; Gaus, Katharina; Brodnicki, Thomas C.
Source:
Molecular Immunology 2016
ISSN:
0161-5890
Subject:
viability,   etc   ; autoimmune diseases; autoimmunity; disease resistance; lipid composition; lymphocyte proliferation; lymphocytes; macrophages; mice; phosphatidylserines; plasma membrane; serine; sphingolipids;     Show all 13 Subjects
Abstract:
... During immune cell activation, serine-derived lipids such as phosphatidylserine and sphingolipids contribute to the formation of protein signaling complexes within the plasma membrane. Altering lipid composition in the cell membrane can subsequently affect immune cell function and the development of autoimmune disease. Serine incorporator 1 (SERINC1) is a putative carrier protein that facilitates ...
DOI:
10.1016/j.molimm.2016.12.007

23. Down-regulation of Homer1 attenuates t-BHP-induced oxidative stress through regulating calcium homeostasis and ER stress in brain endothelial cells

Author:
Guo, Zhen-Yu; Zhang, Ya-Hong; Xie, Guo-Qiang; Liu, Chong-Xiao; Zhou, Ren; Shi, Wei
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
cell viability,   etc   ; adenosine triphosphate; animal models; apoptosis; brain; calcium; dose response; edema; endoplasmic reticulum; endothelial cells; genes; homeostasis; image analysis; inositols; lactate dehydrogenase; lipid peroxidation; membrane potential; mitochondrial membrane; mortality; nervous system diseases; oxidative stress; receptors; small interfering RNA; toxicity;     Show all 24 Subjects
Abstract:
... Endothelial dysfunction in brain endothelial cells contributes to vasogenic cerebral edema and increased mortality after various neurological diseases. The postsynaptic density protein Homer1 plays an important role in neuronal synaptic activity and is extensively involved in neurological disorders. The present study investigated the role of Homer1 in modulating cell survival using an in vitro end ...
DOI:
10.1016/j.bbrc.2016.07.012

24. 137 dynamic shift in cytoplasmic lipids in cat oocytes during ovarian follicle development

Author:
Thongkittidilok, C.; Li, Y.; Wildt, D.; Songsasen, N.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 198-199
ISSN:
1031-3613
Subject:
viability,   etc   ; Felidae; cats; computer software; databases; desorption; developmental stages; embryogenesis; follicular development; glycerophosphates; goats; granulosa cells; in vitro culture; lipid composition; mass spectrometry; oocytes; principal component analysis; rats; spaying; species identification; temporal variation; Western Australia;     Show all 22 Subjects
Abstract:
... Cytoplasmic lipids play key roles during oocyte and embryo development. However, there is little information on the changes in lipid types during intraovarian follicular and oocyte growth. Here, we used matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry to understand lipid composition in the cat oocyte during differing stages of folliculogenesis. Follicles at ...
DOI:
10.1071/RDv28n2Ab136

25. Effect of ciprofloxacin on biological nitrogen and phosphorus removal from wastewater

Author:
Yi, Kaixin; Wang, Dongbo; QiYang,; Li, Xiaoming; Chen, Hongbo; Sun, Jian; An, Hongxue; Wang, Liqun; Deng, Yongchao; Liu, Jun; Zeng, Guangming
Source:
The Science of the total environment 2016
ISSN:
0048-9697
Subject:
viability,   etc   ; activated sludge; acute exposure; adverse effects; ciprofloxacin; denitrification; denitrifying bacteria; glycogen; high-throughput nucleotide sequencing; long term experiments; nitrite reductase; nitrogen; phosphorus; polyhydroxyalkanoates; wastewater;     Show all 15 Subjects
Abstract:
... In this work, both short-term and long-term experiments were therefore conducted to assess the effects of ciprofloxacin (0.2 and 2mg·L−1) on wastewater nutrient removal. The results showed that both levels of ciprofloxacin had no acute and chronic adverse effects on the surface integrity and viability of activated sludge. Short-term exposure to all the ciprofloxacin levels induced negligible influ ...
DOI:
10.1016/j.scitotenv.2017.06.215

26. Effect of different vitrification solutions and cryodevices on viability and subsequent development of buffalo oocytes vitrified at the germinal vesicle (GV) stage

Author:
El-Shalofy, Amr S.; Moawad, Adel R.; Darwish, Gamal M.; Ismail, Sayed T.; Badawy, Aly B.A.; Badr, Magdy R.
Source:
Cryobiology 2016
ISSN:
0011-2240
Subject:
viability,   etc   ; blastocyst; buffaloes; cattle; cryopreservation; dimethyl sulfoxide; embryo culture; ethylene glycol; females; germinal vesicle; glycerol; in vitro fertilization; slaughterhouses; survival rate; vitrification;     Show all 15 Subjects
Abstract:
... The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryo ...
DOI:
10.1016/j.cryobiol.2016.11.010

27. Effect of natural mating or laparoscopic artificial insemination in superovulated Santa Inês ewes on superovulatory response, fertility and embryo viability

Author:
Lima, J. T. M.; Fonseca, J. F.; Balaro, M. F. A.; Esteves, L. V.; Ascoli, F. O.; Leite, C. R.; Ribeiro, A. C. S.; Delgado, K. F.; Souza-Fabjan, J. M. G.; Filho, R. A. Torres; Brandão, F. Z.
Source:
Animal production science 2016 v.56 no.9 pp. 1463-1468
ISSN:
1836-0939
Subject:
viability,   etc   ; artificial insemination; cloprostenol; corpus luteum; cross-over studies; equine chorionic gonadotropin; estrus; ewes; follicle-stimulating hormone; gonadotropin-releasing hormone; laparoscopy; natural mating; oocytes; ovulation; rams; semen; superovulation; ultrasonography;     Show all 18 Subjects
Abstract:
... This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 30 ...
DOI:
10.1071/AN14827

28. Effects of cryopresevation on the epigenetic profile of cells

Author:
Chatterjee, A.; Saha, D.; Niemann, H.; Gryshkov, O.; Glasmacher, B.; Hofmann, N.
Source:
Cryobiology 2016
ISSN:
0011-2240
Subject:
cell viability,   etc   ; DNA methylation; cryopreservation; epigenetics; freezing; histones; long term effects; storage; survival rate;     Show all 9 Subjects
Abstract:
... Effective cryopreservation protocols are essential for long-term storage of cells and their subsequent clinical application. Freezing protocols are generally considered as safe; however, putative effects on epigenetic marks have not yet been studied in detail. While post-thaw cell survival rates have been used to evaluate the success of cryopreservation protocols, increasing evidence suggests that ...
DOI:
10.1016/j.cryobiol.2016.12.002

29. Effects of Extenders on Fresh and Freezing Semen of Boer Goat

Author:
Yodmingkwan, Phakatip; Guntaprom, Somchit; Jaksamrit, Juggrid; Lertchunhakiat, Krittiya
Source:
Agriculture and Agricultural Science Procedia 2016 v.11 pp. 125-130
ISSN:
2210-7843
Subject:
viability,   etc   ; Boer (goat breed); artificial vagina; bucks; cryopreservation; egg yolk; freezing; goats; semen; soybeans; sperm motility;     Show all 11 Subjects
Abstract:
... The aim of this study was to evaluate the effect of soybean lecithin and egg yolk on semen quality of Boer goats. Semen of the three Boer bucks was collected using artificial vagina. In the first experiment, the semen samples were preserved in liquid state and subsequently were diluted with Tris-fructose-citric acid (TFC), Tris-fructose-citric acid + 1.5% soybean lecithin (TFCSL) and Tris-fructose ...
DOI:
10.1016/j.aaspro.2016.12.021

30. 76 effects of reduced environmental light on the in vitro maturation of pig oocytes

Author:
Parra-Forero, L. Y.; Góngora, A.; Romo-García, S.; López Damian, E. P.; Mendoza, G. D.; Guevara, J. A.; García-Contreras, A.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 167-168
ISSN:
1031-3613
Subject:
viability,   etc   ; Duroc; anaphase; blastocyst; culture media; fluorescence; formaldehyde; germinal vesicle; gilts; humans; hyaluronoglucosaminidase; meiosis; metaphase; red light; slaughter; sodium chloride; solar radiation; streptomycin; sulfates; t-test; telophase; ultraviolet radiation;     Show all 22 Subjects
Abstract:
... The effects of light on the steps of embryo manipulation have been described in several species, including humans. There are reports in which exposure of these cells to UV rays from sunlight affects them epigenetically, which could lead to meiotic arrest that would prevent normal maturation from the germinal vesicle (GV) to metaphase II (MII) stage, which would compromise subsequent embryo viabili ...
DOI:
10.1071/RDv28n2Ab75

31. 163 effects of serum and l-carnitine on development and cryotolerance of bovine embryos produced in vitro

Author:
Zolini, A.; Carrascal-Triana, E. L.; Ruiz, A.; Penitente-Filho, J. M.; Hansen, P. J.; Alves Torres, C. A.; Block, J.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 211-212
ISSN:
1031-3613
Subject:
viability,   etc   ; blastocyst; carbon dioxide; cattle; dithiothreitol; embryogenesis; ethylene glycol; fetal bovine serum; freezing; hatching; oocytes; oxygen; regression analysis;     Show all 13 Subjects
Abstract:
... Cryotolerance of bovine embryos produced in vitro (PIV) can be improved by l-carnitine. The objective of the present study was to determine whether the optimal concentration of l-carnitine is dependent on serum. Bovine embryos were produced in vitro with abattoir-derived oocytes. After fertilization (Day 0), oocytes (n=2768) were randomly assigned in a 2×4 factorial design to culture in SOF-BE1 me ...
DOI:
10.1071/RDv28n2Ab162

32. 35 effects of thawing temperature of frozen semen on viability of refrozen and thawed chickso (korean brindle cattle) and korean albino cattle spermatozoa

Author:
Kim, S. W.; Choe, C. Y.; Kim, D. K.; Choi, A. R.; Seong, H. H.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 147-148
ISSN:
1031-3613
Subject:
viability,   etc   ; albino; cattle; cooling; cryopreservation; extinction; freeze-thaw cycles; freezing; germplasm; risk; semen; sperm concentration; spermatozoa; temperature; thawing;     Show all 15 Subjects
Abstract:
... Germplasm cryopreservation from a desired species with agricultural and genetic importance would protect them from the risk for extinction. Semen freezing from Korean native cattle would be a good approach for protecting genetic resources due to their limited numbers. It has been known that sperm could resist cryo-damages by freeze-thaw cycles. Thus, we performed 2 refreezing experiments with diff ...
DOI:
10.1071/RDv28n2Ab34

33. 91 embryo sugar consumption during in vitro development

Author:
Rubessa, M.; Ambrosi, A.; Polkoff, K. M.; Denmark, S. E.; Wheeler, M. B.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 175
ISSN:
1031-3613
Subject:
viability,   etc   ; acetates; air; analysis of variance; animal reproduction; blastocyst; carbon dioxide; cattle; culture media; deuterium oxide; developmental stages; energy; glycogen; glycolysis; linear models; metabolites; myo-inositol; nuclear magnetic resonance spectroscopy; oocytes; oxygen; pyruvic acid; sodium; triacylglycerols; tricarboxylic acid cycle; zygote; California;     Show all 26 Subjects
Abstract:
... Despite the presence of glycogen and triglycerides as energy reserves in bovine embryos, the viability of embryonic cells in culture is maintained primarily by energetic substrates present in the culture medium. The aim of this experiment was evaluate the embryo metabolism during three important stages of development: Days 1 to 3, Days 3 to 5, and Days 5 to 7; before the formation of Barr body, be ...
DOI:
10.1071/RDv28n2Ab91

34. Endosulfan inhibits proliferation through the Notch signaling pathway in human umbilical vein endothelial cells

Author:
Wei, Jialiu; Zhang, Lianshuang; Ren, Lihua; Zhang, Jin; Yu, Yang; Wang, Ji; Duan, Junchao; Peng, Cheng; Sun, Zhiwei; Zhou, Xianqing
Source:
Environmental pollution 2016
ISSN:
0269-7491
Subject:
cell viability,   etc   ; acetylcysteine; apoptosis; blood; coagulation; endosulfan; human umbilical vein endothelial cells; lactate dehydrogenase; malondialdehyde; microfilaments; microtubules; mitosis; oxidative stress; reactive oxygen species; signal transduction; toxicity;     Show all 16 Subjects
Abstract:
... Our previous research showed that endosulfan triggers the extrinsic coagulation pathway by damaging endothelial cells and causes hypercoagulation of blood. To identify the mechanism of endosulfan-impaired endothelial cells, we treated human umbilical vein endothelial cells (HUVECs) with different concentrations of endosulfan, with and without an inhibitor for Notch, N-[N-(3, 5-difluorophenacetyl)- ...
DOI:
10.1016/j.envpol.2016.08.083

35. 88 enrichment of culture medium with crocetin improves in vitro embryo development in cattle

Author:
Zullo, G.; Tamayo Palacio, J. E.; De Canditiis, C.; Longobardi, V.; Salzano, A.; Albero, G.; Gasparrini, B.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 173
ISSN:
1031-3613
Subject:
viability,   etc   ; air; analysis of variance; animal reproduction; antioxidant activity; blastocyst; carbon dioxide; cattle; culture media; developmental stages; dose response; embryogenesis; enrichment culture; in vitro fertilization; oocytes; oxidative stress; oxygen; saffron; zygote;     Show all 19 Subjects
Abstract:
... The high incidence of developmental failure of bovine in vitro-produced embryos is due to suboptimal culture conditions that induce oxidative stress. Indeed, increased oxidative stress is one of the main factors affecting in vitro mammalian embryo development, decreasing the viability of IVP embryos. It is known that saffron has a powerful antioxidant capacity, mainly due to its active components ...
DOI:
10.1071/RDv28n2Ab87

36. Evaluation of Pseudoraphis spinescens (Poaceae) seed bank from Barmah Forest floodplain

Author:
Durant, Rebecca A.; Nielsen, Daryl L.; Ward, Keith A.
Source:
Australian journal of botany 2016 v.64 no.8 pp. 669-677
ISSN:
0067-1924
Subject:
viability,   etc   ; Poaceae; drought; floodplains; forests; germination; sediments; seeds; wetland plants; Australia;     Show all 10 Subjects
Abstract:
... Use of environmental water allocations to conserve floodplain and wetland plants is becoming an increasingly common management tool. In south-eastern Australia, Pseudoraphis spinescens (R.Br.) Vickery has historically covered extensive areas within Barmah Forest where it is deemed to be ecologically significant and of high conservation value. The areal extent of P. spinescens significantly decline ...
DOI:
10.1071/BT15288

37. Evidence for improved pollen viability as the mechanism for film antitranspirant mitigation of drought damage to wheat yield

Author:
Weerasinghe, Minuka M.; Kettlewell, Peter S.; Grove, Ivan G.; Hare, Martin C.
Source:
Crop & pasture science 2016 v.67 no.2 pp. 137-146
ISSN:
1836-0947
Subject:
viability,   etc   ; climatic zones; cultivars; drought; field experimentation; grain yield; irrigation; meiosis; pollen; rain; wheat;     Show all 11 Subjects
Abstract:
... Application of film antitranspirant to wheat during late stem extension reduces drought damage to yield, but the mechanism is unknown. Field experiments under rain shelters were conducted over 3 years to test the hypothesis that film antitranspirant applied before meiosis alleviates drought-induced losses of pollen viability, grain number and yield. The film antitranspirant di-1-p-menthene was app ...
DOI:
10.1071/CP15356

38. 187 exploitation of in vitro capacitation for nanoparticle incorporation within mammalian spermatozoa

Author:
Myles, L.; Durfey, C.; Ryan, P.; Willard, S.; Feugang, J.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 224
ISSN:
1031-3613
Subject:
viability,   etc   ; Agricultural Research Service; bioluminescence; boars; caffeine; carbon dioxide; dried milk; heparin; image analysis; membrane fluidity; nanoparticles; plasma membrane; quantum dots; semen; sperm motility; spermatozoa; tissues; toxicity; transmission electron microscopes; transmission electron microscopy;     Show all 20 Subjects
Abstract:
... Migration and interactions of mammalian gametes occur in deep body tissues after mating, rendering difficult any in situ noninvasive evaluation of their performances with current methods. In our effort to develop an effective and real-time in vivo imaging approach, we have successfully labelled porcine gametes with self-illuminating bioluminescent and red-shifted quantum dot nanoparticles (QD) in ...
DOI:
10.1071/RDv28n2Ab186

39. Fertility response of artificial insemination methods in sheep with fresh and frozen-thawed semen

Author:
Masoudi, Reza; Zare Shahneh, Ahmad; Towhidi, Armin; Kohram, Hamid; Akbarisharif, Abbas; Sharafi, Mohsen
Source:
Cryobiology 2016
ISSN:
0011-2240
Subject:
viability,   etc   ; adverse effects; artificial insemination; cryopreservation; egg yolk; ewes; freezing; lambing rate; lipid peroxidation; parturition; pregnancy rate; rams; reproductive performance; semen; soybeans; spermatozoa;     Show all 16 Subjects
Abstract:
... The aim of this study was to evaluate the fertility response of artificial insemination (AI) methods with fresh and frozen sperm in sheep. In experiment 1, one hundred and fifty fat tailed Zandi ewes were assigned into 3 equal groups and inseminated with three AI methods consisting of vaginal, laparoscopic and trans-cervical AI with fresh semen. In experiment 2, a factorial study (3 AI methods × 2 ...
DOI:
10.1016/j.cryobiol.2016.11.012

40. Five new koumine-type alkaloids from the roots of Gelsemium elegans

Author:
Zhang, Wei; Zhang, Sheng-Yuan; Wang, Gui-Yang; Li, Ni-Ping; Chen, Min-Feng; Gu, Ji-Hong; Zhang, Dong-Mei; Wang, Lei; Ye, Wen-Cai
Source:
Fitoterapia 2016
ISSN:
0367-326X
Subject:
viability,   etc   ; Gelsemium; alkaloids; circular dichroism spectroscopy; neoplasm cells; nuclear magnetic resonance spectroscopy; roots; spectral analysis;     Show all 8 Subjects
Abstract:
... Five new koumine-type alkaloids (1–5) along with six known ones were isolated from the roots of Gelsemium elegans. Their structures with absolute configurations were elucidated on the basis of NMR spectroscopy and electronic circular dichroism spectral analyses. The inhibitory effects of compounds 1–11 on the viability of three tumor cell lines (A-649, HepG2, and HuH7) were evaluated by the MTT as ...
DOI:
10.1016/j.fitote.2017.03.004

41. Genetic compatibility among morphotypes of Vigna lanceolata and implications for breeding improved cultivars

Author:
Lawn, R. J.; Vu, Hang T. T.; Bielig, L. M.; Kilian, A.
Source:
Crop & pasture science 2016 v.67 no.7 pp. 739-750
ISSN:
1836-0947
Subject:
viability,   etc   ; Vigna; coasts; cover crops; cowpeas; cross pollination; cultivars; gene pool; genetic variation; geographical distribution; hybrids; ideotypes; mung beans; pastures; progeny; reciprocal crosses; sowing; understory; Australia;     Show all 19 Subjects
Abstract:
... As well as being part of the wider gene pool of cultivated species such as mungbean and cowpea, Vigna lanceolata Benth. is of agronomic interest as a potentially useful species in its own right. It is widely adapted across northern Australia from the coast to inland desert regions, and possesses attributes that make it potentially suited as a ley, cover crop or pasture legume in seasonally arid tr ...
DOI:
10.1071/CP16014

42. Ghrelin ameliorates the human alveolar epithelial A549 cell apoptosis induced by lipopolysaccharide

Author:
Huang, Chunrong; Zheng, Haichong; He, Wanmei; Lu, Guifang; Li, Xia; Deng, Yubin; Zeng, Mian
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
cell viability,   etc   ; apoptosis; caspase-3; epithelial cells; epithelium; ghrelin; humans; lipopolysaccharides; mitogen-activated protein kinase; non-specific serine/threonine protein kinase; protective effect;     Show all 11 Subjects
Abstract:
... Ghrelin is a gastric acyl-peptide that plays an inhibitory role in cell apoptosis. Herein we investigate the protective effects of ghrelin in LPS-induced apoptosis of human alveolar epithelial A549 cells, along with the possible molecular mechanisms. LPS exposure impaired cell viability and increased apoptosis of A549 cells significantly in concentration- and time-dependent manners embodied in inc ...
DOI:
10.1016/j.bbrc.2016.04.074

43. Green production of microalgae-based silver chloride nanoparticles with antimicrobial activity against pathogenic bacteria

Author:
da Silva Ferreira, Veronica; ConzFerreira, Mateus Eugenio; Lima, Luís Maurício T.R.; Frasés, Susana; de Souza, Wanderley; Sant’Anna, Celso
Source:
Enzyme and Microbial Technology 2016
ISSN:
0141-0229
Subject:
cell viability,   etc   ; Chlorella vulgaris; DNA; Fourier transform infrared spectroscopy; Klebsiella pneumoniae; Staphylococcus aureus; X-ray diffraction; absorbance; anti-infective agents; antibacterial properties; bacteria; chlorine; color; dose response; energy-dispersive X-ray analysis; microalgae; nanoparticles; nanosilver; pathogens; proteins; screening; silver; silver chloride; silver nitrate; transmission electron microscopy; ultraviolet-visible spectroscopy;     Show all 26 Subjects
Abstract:
... Silver nanoparticles are powerful antimicrobial agents. Here, the synthesis of silver chloride nanoparticles (AgCl-NPs) was consistently evidenced from a commercially valuable microalgae species, Chlorella vulgaris. Incubation of C. vulgaris conditioned medium with AgNO3 resulted in a medium color change to yellow/brown (with UV–vis absorbance at 415nm), indicative of silver nanoparticle formation ...
DOI:
10.1016/j.enzmictec.2016.10.018

44. Impact of heat on seed germination of three perennial grasses in the semiarid region in Central Argentina

Author:
Kin, Alicia G.; Suárez, Carla E.; Chirino, Claudia C.; Ávila, Patricia L.; Morici, Ernesto F. A.
Source:
Australian journal of botany 2016 v.64 no.5 pp. 451-455
ISSN:
0067-1924
Subject:
viability,   etc   ; Nassella tenuis; adverse effects; buried seeds; exposure duration; grasses; heat; heat stress; heat treatment; indigenous species; perennials; range management; seed germination; semiarid zones; soil; temperature; Argentina;     Show all 17 Subjects
Abstract:
... Fire is an important factor affecting the viability and germination of seeds in the soil seed bank. We evaluated the effects of heat on germination and seed viability of three native perennials winter grasses of central Argentina. Seeds of two palatable grasses Piptochaetium napostaense (Speg.) Hack, Nassella tenuis (Phil.) Barkworth and a non-palatable Jarava ichu Ruiz & Pav. were exposed to diff ...
DOI:
10.1071/BT15238

45. 86 improved bovine embryo production using novel in vitro culture systems

Author:
Pryor, J. H.; Hasler, J. F.; Strøbech, L.; Avery, B.; Hashem, N.; Menges, S.; Long, C. R.; Shewfelt, G.; Looney, C. R.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 172
ISSN:
1031-3613
Subject:
viability,   etc   ; blastocyst; business enterprises; carbon dioxide; cattle; cleaning; cold; cultured cells; embryogenesis; fetal bovine serum; glutamine; hatching; heat stress; in vitro fertilization; methanol; oils; oocytes; oxygen; penicillins; polysorbates; pyruvic acid; salts; seasonal variation; sodium; streptomycin; t-test; temperature; ultraviolet radiation; zygote;     Show all 29 Subjects
Abstract:
... Development and testing of new embryo production components is important to improve the outcome following in vitro production of bovine embryos. The objective of this study was to compare media used in two bovine embryo production systems (control and EmbryoTrans Biotech: ETB). In Exp. 1, abattoir-derived cumulus-oocyte complexes were randomly assigned and in vitro matured (IVM) in either control ...
DOI:
10.1071/RDv28n2Ab85

46. Inactivation of Escherichia coli Planktonic Cells by Multi-walled Carbon Nanotubes in Suspensions: Effect of Surface Functionalization Coupled with Medium Nutrition Level

Author:
Chi, Mu-Fan; Wu, Wei-Ling; Du, Yuchin; Chin, Ching-Ju M.; Lin, Chu-Ching
Source:
Journal of hazardous materials 2016
ISSN:
0304-3894
Subject:
viability,   etc   ; Escherichia coli; acid treatment; adsorption; antibacterial properties; carbon nanotubes; cell membranes; cytotoxicity; direct contact; ecotoxicology; electrostatic interactions; nutrients; nutrition; organic matter; physical properties; plankton;     Show all 16 Subjects
Abstract:
... While earlier studies have identified the antibacterial activity of carbon nanotubes (CNTs) and proposed that cell membrane damage by direct contact with CNTs is likely the main toxicity mechanism, the relative importance of chemical versus physical properties of CNTs in controlling their bacterial cytotoxicity is understudied. Given that CNT is commonly modified via acid treatment to enhance its ...
DOI:
10.1016/j.jhazmat.2016.07.013

47. Inhibition of phosphoserine phosphatase enhances the anticancer efficacy of 5-fluorouracil in colorectal cancer

Author:
Li, Xin; Xun, Zhe; Yang, Yong
Source:
Biochemical and biophysical research communications 2016
ISSN:
0006-291X
Subject:
cell viability,   etc   ; colorectal neoplasms; fluorouracil; glucose; glycine (amino acid); models; oxidative stress; patients; phosphoserine phosphatase; reactive oxygen species; serine; therapeutics;     Show all 12 Subjects
Abstract:
... Most colorectal cancer (CRC) cell lines are identified to overexpress phosphoserine phosphatase (PSPH), which regulates the intracellular synthesis of serine and glycine, and supports tumor growth. In this study, the effect of PSPH on 5-fluorouracil (5-FU) efficacy was evaluated. CRC cells exposed to 5-FU acquire metabolic remodeling, resulting in increased glucose flux for PSPH-mediated serine sy ...
DOI:
10.1016/j.bbrc.2016.06.112

48. 170 IN VITRO EMBRYO PRODUCTION AND APOPTOSIS DETECTION USING BOVINE OOCYTES MATURED WITH IGF-I OR IGF-LongR3

Author:
De Chico, P. L. M.; Recalde, E. C. S.; Ikeda, T. L.; Sudano, M. J.; Landim-Alvarenga, F.
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 215
ISSN:
1031-3613
Subject:
viability,   etc   ; DNA; amikacin; analysis of variance; apoptosis; blastocyst; carbon dioxide; cattle; computer software; cytoplasm; droplets; embryogenesis; fetal bovine serum; fluorescein; fluorescence; follicle-stimulating hormone; humans; insulin; insulin-like growth factor I; least squares; luteinizing hormone; mineral oil; models; myo-inositol; oocytes; penicillins; polyvinyl alcohol; polyvinylpyrrolidone; pyruvic acid; slaughterhouses; sodium chloride; streptomycin; sulfates; temperature;     Show all 34 Subjects
Abstract:
... The aim of this study was to compare the differential effects of the addition of IGF-LongR3 or insulin-like growth factor-1 (IGF-1), during in vitro maturation (IVM) of bovine oocytes on embryonic development, by analysing embryonic viability and apoptosis. Ovaries collected at a slaughterhouse were transported in saline solution (NaCl 0.9%) at 38°C. Follicles of 2–8mm of diameter were aspirated. ...
DOI:
10.1071/RDv28n2Ab169

49. L- Carnitine in rooster semen cryopreservation: Flow cytometric, biochemical and motion findings for frozen-thawed sperm

Author:
Fattah, A.; Sharafi, M.; Masoudi, R.; Shahverdi, A.; Esmaeili, V.
Source:
Cryobiology 2016
ISSN:
0011-2240
Subject:
viability,   etc   ; apoptosis; artificial insemination; cryopreservation; flocks; flow cytometry; lipid peroxidation; mitochondria; roosters; semen; spermatozoa;     Show all 11 Subjects
Abstract:
... Rooster semen cryopreservation is not efficient for artificial insemination in breeder flocks. lL-carnitine (LC) has been evaluated for effectiveness in cryopreservation media on the characteristics of rooster sperm after freeze-thawing. Motility characteristics, membrane functionality, abnormal morphology, apoptotic like changes, mitochondria activity and lipid peroxidation of rooster sperms were ...
DOI:
10.1016/j.cryobiol.2016.10.009

50. L- carnitine is a survival factor for chilled storage of rooster semen for a long time

Author:
Fattah, A.; Sharafi, M.; Masoudi, R.; Shahverdi, A.; Esmaeili, V.
Source:
Cryobiology 2016
ISSN:
0011-2240
Subject:
viability,   etc   ; artificial insemination; cold storage; cooling; flocks; lipid peroxidation; mitochondria; roosters; semen; sperm motility; spermatozoa;     Show all 11 Subjects
Abstract:
... Rooster sperm is sensitive to cooling, which restricts procedures to store sperms for extended periods of time for artificial insemination of commercial flocks. This study was conducted to evaluate the suitability of adding L-carnitine (LC) to chilled-storage of rooster sperm and its effects on sperm quality parameters and its fertility potential during storage at 5 °C. Pooled semen from roosters ...
DOI:
10.1016/j.cryobiol.2016.12.011