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- Chen, Shuaiyin, et al. Show all 7 Authors
- Biotechnology letters 2016 v.38 no.11 pp. 1911-1916
- Escherichia coli; Helicobacter pylori; Lactococcus lactis; Western blotting; antigens; edible vaccines; gene fusion; humoral immunity; immune response; mice; molecular weight; nisin; oral vaccination; plasmids; vaccine development
- ... OBJECTIVES: To develop orally administrated anti-Helicobacter pylori vaccination, a Lactococcus lactis strain was genetically constructed for fusion expression of H. pylori protective antigens HpaA and Omp22. RESULTS: The fusion gene of omp22 and hpaA with an adapter encoding three glycines was cloned from a plasmid pMAL-c2x-omp22-hpaA into Escherichia coli MC1061 and L. lactis NZ3900 successively ...
- Chen, Shuaiyin, et al. Show all 8 Authors
- Biotechnology letters 2016 v.38 no.12 pp. 2169-2175
- Helicobacter pylori; Lactococcus lactis; Western blotting; animal experimentation; antibodies; antigens; blood serum; genes; immune response; immunoglobulin G; lipoproteins; mice; oral vaccination; polyacrylamide gel electrophoresis; vaccines
- ... OBJECTIVES: To produce and deliver Helicobacter pylori lipoprotein Lpp20 via using Lactococcus lactis with aim of developing an efficient way to use this protective antigen in vaccine formulation. RESULTS: An engineered L. lactis strain carrying the lpp20 gene from H. pylori was constructed. The inducible expression of Lpp20 in L. lactis was detected as a 20 kDa intracellular protein by SDS-PAGE. ...