Jump to Main Content
- Niwa, Hidekazu, et al. Show all 4 Authors
- Rhodococcus equi; bacteria; bacterial culture; equipment; foals; gene amplification; genes; loop-mediated isothermal amplification; pneumonia; quantitative polymerase chain reaction; veterinarians; virulence
- ... Rhodococcus equi is the most important causative bacterium of severe pneumonia in foals. We report herein the development of a specific loop-mediated isothermal amplification (LAMP) assay, which targets a gene encoding vapA for detecting virulent R. equi. The detection limit of the LAMP assay was 10⁴ colony forming units (CFU)/mL, which was equal to 10 CFU/reaction. The clinical efficacy of the LA ...
- Niwa, Hidekazu, et al. Show all 9 Authors
- Anoplocephala perfoliata; Gasterophilus intestinalis; Parascaris equorum; Salmonella Abortusequi; Strongylus vulgaris; agglutination; aneurysm; bacteria; blood; bone marrow; draft horses; enzootic diseases; farms; intestines; larvae; lymph nodes; mesenteric arteries; necropsy; parasitoses; pathogens; plasmids; pulsed-field gel electrophoresis; serotypes; thrombosis; virulence; Japan
- ... Salmonella enterica subsp. enterica serovar Abortusequi is a pathogen restricted to horses. Our investigation targeted 4 draft horses (9–10 months old) kept on a Japanese farm that had suffered an outbreak of S. Abortusequi abortion. The 4 horses were suspected to be carriers of the bacterium owing to their high agglutination titers (≥1:2,560) in tube agglutination testing. The owners’ on-farm obs ...