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1. VqDUF642, a gene isolated from the Chinese grape Vitis quinquangularis, is involved in berry development and pathogen resistance

Author:
Wang, Yuejin,   et al.   ; Xie, Xiaoqing;     Show all 2 Author
Source:
Planta 2016 v.244 no.5 pp. 1075-1094
ISSN:
0032-0935
Subject:
Botrytis cinerea; Uncinula necator; Vitis heyneana; cell walls; complementary DNA; gene overexpression; grapes; open reading frames; pathogens; phylogeny; plant development; plant growth; proteins; proteomics; sequence alignment; tobacco; tomatoes; transgenes; transgenic plants
Abstract:
... MAIN CONCLUSION : The DUF642 gene VqDUF642 , isolated from the Chinese grape species V. quinquangularis accession Danfeng-2, participates in berry development and defense responses against Erysiphe necator and Botrytis cinerea. The proteins with domains of unknown function 642 (DUF642) comprise a large protein family according to cell wall proteomic analyses in plants. However, the works about fun ...
DOI:
10.1007/s00425-016-2569-4

2. Isolation and analysis of differentially expressed genes during ovule abortion in the seedless grape

Author:
Wang, Yuejin,   et al.   ; Wang, Yanqing; Li, Shuxiu; Zhang, Xutong; Zhang, Chaohong;     Show all 5 Authors
Source:
Scientia horticulturae 2016 v.211 pp. 376-383
ISSN:
0304-4238
Subject:
Vitis vinifera; complementary DNA; expressed sequence tags; gene expression regulation; genes; grapes; ovules; tissues
Abstract:
... The propagation of seedless grapes is currently one of the most important global trends in agricultural production and consumption. However, stenospermocarpy has not been characterized at the molecular level to date. In the present study, cDNA sequence-related amplified polymorphism (SRAP) analysis using 17 SRAP primers was utilized to identify differentially expressed genes during ovule developme ...
DOI:
10.1016/j.scienta.2016.09.014

3. Genetic transformation of grape varieties and rootstocks via organogenesis

Author:
Wang, Yuejin,   et al.   ; Xie, Xiaoqing; Agüero, Cecilia B.; Walker, M. Andrew;     Show all 4 Authors
Source:
Plant cell, tissue, and organ culture 2016 v.126 no.3 pp. 541-552
ISSN:
0167-6857
Subject:
Vitis riparia; Vitis rupestris; Vitis vinifera; cultivars; cytokinins; genetic transformation; genotype; grapes; green fluorescent protein; in vitro studies; kanamycin; leaves; meristems; naphthaleneacetic acid; organogenesis; roots; rootstocks
Abstract:
... A protocol was standardized to regenerate six grape cultivars through meristematic bulk (MB) induction, which was used for genetic transformation. Meristematic bulk induction worked best with Vitis vinifera ‘Thompson Seedless’ (98.4 %), followed by ‘Chardonnay’ (97.6 %), ‘Redglobe’ (90.2 %) and ‘Cabernet Sauvignon’ (86.2 %), and was less successful with Vitis rupestris ‘St. George’ (85.4 %) and ‘1 ...
DOI:
10.1007/s11240-016-1023-4

4. Functional analysis of the fruit-specific promoter of VqSTS6 from the Chinese wild grape, Vitis quinquangularis

Author:
Wang, Yuejin,   et al.   ; He, Jing; Wang, Dan; Zhang, Jianxia;     Show all 4 Authors
Source:
Agri Gene 2016 v.1 pp. 38-45
ISSN:
2352-2151
Subject:
Solanum lycopersicum; Vitis heyneana; Vitis vinifera; beta-glucuronidase; biochemical pathways; fruiting; gene expression; genes; grapes; leaves; metabolites; polyketide synthases; promoter regions; resveratrol; roots; stems; tomatoes; trihydroxystilbene synthase
Abstract:
... Stilbene synthase plays important roles in the secondary metabolic pathways of many plants as polyketide synthase. Increased consumption of its metabolite, resveratrol, is highly beneficial to our health. The stilbene synthase 6 gene (VqSTS6) has been obtained from the Chinese wild Vitis quinquangularis accession ‘Danfeng-2’. Our previous studies have shown that this stilbene synthase gene exhibit ...
DOI:
10.1016/j.aggene.2016.06.002

5. Ectopic expression of a grape aspartic protease gene, AP13, in Arabidopsis thaliana improves resistance to powdery mildew but increases susceptibility to Botrytis cinerea

Author:
Wang, Yuejin,   et al.   ; Guo, Rongrong; Tu, Mingxing; Wang, Xianhang; Zhao, Jiao; Wan, Ran; Li, Zhi; Wang, Xiping;     Show all 8 Authors
Source:
Plant science 2016 v.248 pp. 17-27
ISSN:
0168-9452
Subject:
Arabidopsis thaliana; Botrytis cinerea; Pseudomonas syringae pv. tomato; Uncinula necator; Vitis heyneana; Vitis labrusca; Vitis vinifera; aspartic proteinases; bacteria; callose; disease resistance; ethylene; fungi; genes; grapes; methyl jasmonate; pathogens; powdery mildew; promoter regions; salicylic acid; signal transduction; transgenic plants
Abstract:
... The grape aspartic protease gene, AP13 was previously reported to be responsive, in Chinese wild Vitis quinquangularis cv. ‘Shang-24’, to infection by Erysiphe necator, the causal agent of powdery mildew disease, as well as to treatment with salicylic acid in V. labrusca×V. vinifera cv. ‘Kyoho’. In the current study, we evaluated the expression levels of AP13 in ‘Shang-24’ in response to salicylic ...
DOI:
10.1016/j.plantsci.2016.04.006

6. Genetic transformation of a fruit-specific, highly expressed stilbene synthase gene from Chinese wild Vitis quinquangularis

Author:
Wang, Yuejin,   et al.   ; Cheng, Siyan; Xie, Xiaoqing; Xu, Yan; Zhang, Chaohong; Wang, Xiping; Zhang, Jianxia;     Show all 7 Authors
Source:
Planta 2016 v.243 no.4 pp. 1041-1053
ISSN:
0032-0935
Subject:
Uncinula necator; Vitis heyneana; Western blotting; breeding; disease resistance; fruit quality; gene expression; genes; genetic transformation; grapes; pathogens; powdery mildew; quantitative polymerase chain reaction; resveratrol; reverse transcriptase polymerase chain reaction; transgenic plants; trihydroxystilbene synthase
Abstract:
... MAIN CONCLUSION : The stilbene synthase gene VqSTS6, from Chinese wild type Vitis quinquangularis accession Danfeng-2, increases the resveratrol content and pathogen resistance of transgenic plants of V. vinifera Thompson Seedless. This study successfully created transgenic plants of V. vinifera Thompson Seedless which overexpressed VqSTS6, cloned from Chinese wild type V. quinquangularis accessio ...
DOI:
10.1007/s00425-015-2459-1

7. Expression of a grape (Vitis vinifera) bZIP transcription factor, VlbZIP36, in Arabidopsis thaliana confers tolerance of drought stress during seed germination and seedling establishment

Author:
Wang, Yuejin,   et al.   ; Tu, Mingxing; Wang, Xianhang; Feng, Tongying; Sun, Xiaomeng; Wang, Yaqiong; Huang, Li; Gao, Min; Wang, Xiping;     Show all 9 Authors
Source:
Plant science 2016 v.252 pp. 311-323
ISSN:
0168-9452
Subject:
Arabidopsis thaliana; Vitis vinifera; abiotic stress; abscisic acid; cell death; drought; drought tolerance; electrolytes; enzyme activity; genes; grapes; leucine zipper; mature plants; promoter regions; seed germination; seedlings; transcription (genetics); transcription factors; transgenic plants
Abstract:
... Drought is one of the most serious factors that limit agricultural productivity and there is considerable interest in understanding the molecular bases of drought responses and their regulation. While numbers of basic leucine zipper (bZIP) transcription factors (TFs) are known to play key roles in response of plants to various abiotic stresses, only a few group K bZIP TFs have been functionally ch ...
DOI:
10.1016/j.plantsci.2016.08.011