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1. Analysis of Cocoa Proanthocyanidins Using Reversed Phase High-Performance Liquid Chromatography and Electrochemical Detection: Application to Studies on the Effect of Alkaline Processing

Author:
Smithson, Andrew T.; Neilson, Andrew P.,   et al.   ; Stanley, Todd H.; Ananthewaran, Ramaswamy C.; Lambert, Joshua D.;     Show all 5 Authors
Source:
Journal of agricultural and food chemistry 2015 v.63 no.25 pp. 5970-5975
ISSN:
0021-8561
Subject:
alkali treatment; alkalinization; catechin; cocoa powder; color; electrochemistry; epicatechin; models; pH; polymerization; proanthocyanidins; reversed-phase high performance liquid chromatography; sodium hydroxide
Abstract:
... Flavan-3-ols and proanthocyanidins play a key role in the health beneficial effects of cocoa. Here, we developed a new reversed phased high-performance liquid chromatography–electrochemical detection (HPLC-ECD) method for the analysis of flavan-3-ols and proanthocyanidins of degree of polymerization (DP) 2–7. We used this method to examine the effect of alkalization on polyphenol composition o ...
DOI:
10.1021/acs.jafc.5b02661