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1. Acetylcholinesterase Genes in the Glanville Fritillary Butterfly (Melitaea cinxia, Lepidoptera: Nymphalidae)

Author:
Li, Jialian; Luo, Shiqi; Shu, Chang; Xu, Chongren; Wang, Rongjiang
Source:
Journal of the Kansas Entomological Society 2015 v.88 no.3 pp. 340-353
ISSN:
0022-8567
Subject:
Nymphalidae; acetylcholinesterase; amino acid substitution; amino acids; butterflies; carbamates; complementary DNA; genes; habitats; insecticide resistance; insecticides; moths; open reading frames; organophosphorus compounds; pests; phylogeny; proteins; tissues
Abstract:
... Acetylcholinesterase (AChE, EC 3.1.1.7), which is encoded by the ace gene, is a key enzyme in both cholinergic system and other non-neuronal tissues. AChE is the target of organophosphates (OP) and carbamates (CA) insecticides. Although AChEs have been extensively studied in lepidopteran pests, no research has been carried out on butterflies, a major subgroup in Lepidoptera. In this study, we clon ...
DOI:
10.2317/kent-88-03-340-353.1

2. A C-type lectin (LvCTL4) from Litopenaeus vannamei is a downstream molecule of the NF-κB signaling pathway and participates in antibacterial immune response

Author:
Li, Haoyang; Chen, Yonggui; Li, Ming; Wang, Sheng; Zuo, Hongliang; Xu, Xiaopeng; Weng, Shaoping; He, Jianguo; Li, Chaozheng
Source:
Fish & shellfish immunology 2015 v.43 no.1 pp. 257-263
ISSN:
1050-4648
Subject:
Litopenaeus vannamei; RNA interference; Vibrio parahaemolyticus; amino acids; complementary DNA; disulfide bonds; epithelium; genes; hepatopancreas; immune response; intestines; lectins; mortality; open reading frames; pathogens; polypeptides; promoter regions; rapid amplification of cDNA ends; shrimp; signal peptide; signal transduction; transcription factor NF-kappa B; vertebrates
Abstract:
... C-type lectins (CTLs) play multiple roles in innate immune defense against invading pathogens in both vertebrates and invertebrates. In this study, a new C-type lectin gene from pacific white shrimp Litopenaeus vannamei (designated as LvCTL4) was cloned by rapid amplification of the cDNA ends (RACE) method. The full-length cDNA of LvCTL4 was 563 bp with open reading frame (ORF) of 471 bp encoding ...
DOI:
10.1016/j.fsi.2014.12.024

3. A fructose-1,6-biphosphate aldolase gene from Camellia oleifera: molecular characterization and impact on salt stress tolerance

Author:
Zeng, Yanling; Tan, Xiaofeng; Zhang, Lin; Long, Hongxu; Wang, Baoming; Li, Ze; Yuan, Zhen
Source:
Molecular breeding 2015 v.35 no.1 pp. 233
ISSN:
1380-3743
Subject:
Brassica napus; Camellia oleifera; amino acids; complementary DNA; cooking fats and oils; fructose-bisphosphate aldolase; gene overexpression; genes; germination; glyceraldehyde 3-phosphate; glycerol; molecular weight; open reading frames; quantitative polymerase chain reaction; rapid amplification of cDNA ends; roots; salt stress; salt tolerance; seeds; sodium chloride; solutes; stems; stress response; stress tolerance; transgenic plants; trees; China
Abstract:
... Fructose 1,6-bisphosphate aldolase (FBA) catalyzes the reverse cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate (DHAP) and 3-phosphate glyceraldehyde (G-3-P). DHAP acts as a precursor for the synthesis of glycerol, an important osmotically compatible solute under salt stress. Using rapid amplification of cDNA ends, the complete sequence of FBA1 cDNA was obtained from Camellia ...
DOI:
10.1007/s11032-015-0233-5

4. A galectin from shrimp Litopenaeus vannamei is involved in immune recognition and bacteria phagocytosis

Author:
Hou, Fujun; Liu, Yongjie; He, Shulin; Wang, Xianzong; Mao, Aitao; Liu, Zhigang; Sun, Chengbo; Liu, Xiaolin
Source:
Fish & shellfish immunology 2015 v.44 no.2 pp. 584-591
ISSN:
1050-4648
Subject:
Escherichia coli; Litopenaeus vannamei; Micrococcus luteus; Vibrio anguillarum; adaptive immunity; amino acids; antibacterial properties; bacteria; complementary DNA; dimerization; embryogenesis; gene expression regulation; gills; hemocytes; in vivo studies; messenger RNA; open reading frames; phagocytosis; shrimp
Abstract:
... Galectins are conserved family members with β-galactosides affinity that play multiple functions in embryogenesis, development and regulation of innate and adaptive immunity. However, little functional studies were reported in crustaceans. Here, a shrimp Litopenaeus vannamei galectin (LvGal) cDNA was identified with an open reading frame of 1017 bp, which encodes a putative protein of 338 amino ac ...
DOI:
10.1016/j.fsi.2015.03.017

5. A glycosyl hydrolase family 16 gene is responsible for the endogenous production of β-1,3-glucanases within decapod crustaceans

Author:
Linton, Stuart M.; Cameron, Melissa S.; Gray, Michael C.; Donald, John A.; Saborowski, Reinhard; von Bergen, Martin; Tomm, Janina M.; Allardyce, Benjamin J.
Source:
Gene 2015 v.569 pp. 203-217
ISSN:
0378-1119
Subject:
messenger RNA; crabs; complementary DNA; open reading frames; cell walls; Cherax destructor; midgut; microorganisms; amino acid sequences; beta-glucanase; amino acids; hemolymph; Birgus latro; active sites; digestive enzymes; genes; peptides; crayfish; molecular weight
Abstract:
... To identify the gene responsible for the production of a β-1,3-glucanase (laminarinase) within crustacea, a glycosyl hydrolase family 16 (GHF16) gene was sequenced from the midgut glands of the gecarcinid land crab, Gecarcoidea natalis and the freshwater crayfish, Cherax destructor. An open reading frame of 1098bp for G. natalis and 1095bp for C. destructor was sequenced from cDNA. For G. natalis ...
DOI:
10.1016/j.gene.2015.05.056

6. A gonad-stimulating peptide of the crown-of-thorns starfish, Acanthaster planci

Author:
Mita, Masatoshi; Ikeda, Narumi; Haraguchi, Shogo; Tsutsui, Kazuyoshi; Nakano, Yoshikatsu; Nakamura, Masaru
Source:
Invertebrate Reproduction & Development 2015 v.59 no.4 pp. 212-217
ISSN:
2157-0272
Subject:
Acanthaster planci; Asterina pectinifera; amino acids; c-peptide; complementary DNA; coral reefs; corals; messenger RNA; mortality; nerve tissue; oocytes; open reading frames; plant spines; reproduction; signal peptide; spawning
Abstract:
... The crown-of thorns starfish, Acanthaster planci , has been blamed for coral mortality in a large number of coral reef systems in the Indo-Pacific region. Because population outbreaks of A. planci are closely related to reproduction, it is important to examine the mechanism of reproductive control in this starfish. Previously, a relaxin-like gonad-stimulating peptide (RGP) in starfish Asterina pec ...
DOI:
10.1080/07924259.2015.1086828

7. A homolog of Kunitz-type serine protease inhibitor from rock bream, Oplegnathus fasciatus: Molecular insights and transcriptional modulation in response to microbial and PAMP stimulation, and tissue injury

Author:
Bathige, S.D.N.K.; Umasuthan, Navaneethaiyer; Godahewa, G.I.; Jayasinghe, J.D.H.E.; Whang, Ilson; Noh, Jae Koo; Lee, Jehee
Source:
Fish & shellfish immunology 2015 v.46 no.2 pp. 285-291
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Oplegnathus fasciatus; Streptococcus iniae; Takifugu rubripes; amino acids; bacteria; blood cells; bream; complementary DNA; gene expression; immune response; intestines; kidneys; messenger RNA; open reading frames; pathogens; phylogeny; polypeptides; proteinase inhibitors; serine proteinases; tissues; transcription (genetics)
Abstract:
... Serine proteases and their inhibitors play vital roles in diverse biological processes. In this study, we identified and characterized cDNA coding for a Kunitz-type serine protease inhibitor (SPI), which we designated as RbKSPI, in a commercially important species, rock bream. The full-length cDNA sequence of RbKSPI consisted of 2452 bp with an open reading frame (ORF) of 1521 bp encoding a polype ...
DOI:
10.1016/j.fsi.2015.07.004

8. A homologue gene of β-catenin participates in the development of shrimps and immune response to bacteria and viruses

Author:
Xie, Ya-Kai; Ding, Ding; Wang, Hui-Min; Kang, Cui-Jie
Source:
Fish & shellfish immunology 2015 v.47 no.1 pp. 147-156
ISSN:
1050-4648
Subject:
Marsupenaeus japonicus; Staphylococcus aureus; Vibrio anguillarum; adults; amino acids; animal ovaries; apoptosis; bacteria; cell movement; cell proliferation; complementary DNA; genes; gills; hemocytes; immune response; open reading frames; phylogeny; proteins; shrimp; testes; tissues; viruses
Abstract:
... β-Catenin is a multifunctional protein that is involved in many physiological processes, including development, cell proliferation, cell migration, and apoptosis. However, the function of β-Catenin in crustacean is unknown. In this study, the first shrimp homologous gene of β-catenin in Marsupenaeus japonicus (i.e., Mjβ-catenin) was identified and characterized. The full-length of the complementar ...
DOI:
10.1016/j.fsi.2015.08.030

9. A homologue of dermatopontin from Haliotis diversicolor and its response to pathogenic infection

Author:
Wang, Guodong; Zhang, Ziping; Lin, Shi; Zhang, Lili; Wang, Baozhen; Wang, Shuhong; Wang, Yilei
Source:
Aquaculture research 2015 v.46 no.7 pp. 1537-1549
ISSN:
1355-557X
Subject:
Haliotis diversicolor; abalone; adults; amino acid sequences; amino acids; bacteria; bioactive properties; complementary DNA; extracellular matrix; gene expression regulation; genes; gills; hemocytes; immune system; mammals; messenger RNA; open reading frames; proteins; quantitative polymerase chain reaction; sequence analysis; tissue distribution; tissues
Abstract:
... Dermatopontin (DPT), a component of the extracellular matrix, plays important roles in cell‐matrix interactions and matrix assembly. Some studies have revealed that it has more general functions in biological activities. However, its function in molluscs is poorly understood. In this study, a molluscan DPT gene, saDPT2, was cloned from small abalone Haliotis diversicolor. The full‐length cDNA of s ...
DOI:
10.1111/are.12305

10. A Kazal-type serine proteinase inhibitor from Cyclina sinensis is involved in immune response and signal pathway initiation

Author:
Ren, Yipeng; Zhang, Hao; Pan, Baoping; Yan, Chuncai
Source:
Fish & shellfish immunology 2015 v.47 no.1 pp. 110-116
ISSN:
1050-4648
Subject:
RNA interference; Toll-like receptor 2; Vibrio anguillarum; amino acids; bacteria; cDNA libraries; complementary DNA; gene expression; genes; hemocytes; immune response; innate immunity; isoelectric point; messenger RNA; molecular weight; open reading frames; proteinase inhibitors; quantitative polymerase chain reaction; serine proteinases; signal transduction; tissues
Abstract:
... Serine protease inhibitors (SPIs) are an important group of protease inhibitors involved in a variety of biological processes. In the present study, a Kazal-type serine protease inhibitor homolog gene (designated as CsKPI) was identified from a Cyclina sinensis cDNA library. The open reading frame consists of 456 bp and encodes a protein of 151 amino acid residues with a theoretical molecular mass ...
DOI:
10.1016/j.fsi.2015.08.026

11. A manganese superoxide dismutase (MnSOD) from ark shell, Scapharca broughtonii: Molecular characterization, expression and immune activity analysis

Author:
Zheng, Libing; Wu, Biao; Liu, Zhihong; Tian, Jiteng; Yu, Tao; Zhou, Liqing; Sun, Xiujun; Yang, Aiguo
Source:
Fish & shellfish immunology 2015 v.45 no.2 pp. 656-665
ISSN:
1050-4648
Subject:
Gram-negative bacteria; Scapharca; Vibrio anguillarum; acute phase proteins; amino acid sequences; amino acids; antibacterial properties; antioxidant activity; cDNA libraries; complementary DNA; gene expression; hemocytes; hepatopancreas; immune response; manganese; messenger RNA; molluscs; muscles; open reading frames; polypeptides; quantitative polymerase chain reaction; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; superoxide dismutase; tissues
Abstract:
... Manganese superoxide dismutase (MnSOD) is one of the key members of the antioxidant defense enzyme family, however, data regarding to the immune function of MnSOD in mollusks still remain limited now. In this study, a full-length MnSOD cDNA was identified by rapid amplification of cDNA ends (RACE) method from cDNA library of ark shell Scapharca broughtonii (termed SbMnSOD). The cDNA contained an o ...
DOI:
10.1016/j.fsi.2015.05.003

12. A member of the HSP90 family from ovine Babesia in China: molecular characterization, phylogenetic analysis and antigenicity

Author:
GUAN, GUIQUAN; LIU, JUNLONG; LIU, AIHONG; LI, YOUQUAN; NIU, QINGLI; GAO, JINLIANG; LUO, JIANXUN; CHAUVIN, ALAIN; YIN, HONG; MOREAU, EMMANUELLE
Source:
Parasitology 2015 v.142 no.11 pp. 1387-1397
ISSN:
1469-8161
Subject:
Babesia; Western blotting; amino acid sequences; amino acids; complementary DNA; enzyme-linked immunosorbent assay; genes; heat-shock protein 90; new species; open reading frames; phylogeny; polypeptides; rapid amplification of cDNA ends; screening; sheep; China
Abstract:
... Heat shock protein 90 (HSP90) is a key component of the molecular chaperone complex essential for activating many signalling proteins involved in the development and progression of pathogenic cellular transformation. A Hsp90 gene (BQHsp90) was cloned and characterized from Babesia sp. BQ1 (Lintan), an ovine Babesia isolate belonging to Babesia motasi-like group, by screening a cDNA expression libr ...
DOI:
10.1017/S0031182015000797
PubMed:
26156495

13. Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei

Author:
Yang, Hui; Li, Shihao; Li, Fuhua; Wen, Rong; Xiang, Jianhai
Source:
Developmental and comparative immunology 2015 v.51 no.2 pp. 278-286
ISSN:
0145-305X
Subject:
Litopenaeus vannamei; White spot syndrome virus; amino acids; bioinformatics; cell adhesion; cell membranes; complementary DNA; cytoskeleton; gene expression regulation; hemocytes; immunity; immunocytochemistry; mortality; open reading frames; polyclonal antibodies; proteins; rabbits; shrimp; small interfering RNA
Abstract:
... Syndecan is considered to be a multifunctional protein which functions as a cell surface receptor involved in cell adhesion, migration, cytoskeleton organization and differentiation. Previous bioinformatic analysis has revealed that syndecan in shrimp might interact with white spot syndrome virus (WSSV). In the present study, we experimentally studied the function of syndecan in shrimp immunity. T ...
DOI:
10.1016/j.dci.2015.03.013

14. An anti-apoptotic B-cell lymphoma-2 (BCL-2) from Channa striatus: Sequence analysis and delayed and advanced gene expression in response to fungal, bacterial and poly I:C induction

Author:
Arockiaraj, Jesu; Palanisamy, Rajesh; Arasu, Abirami; Sathyamoorthi, Akila; Kumaresan, Venkatesh; Bhatt, Prasanth; Chaurasia, Mukesh Kumar; Pasupuleti, Mukesh; Gnanam, Annie J.
Source:
Molecular Immunology 2015 v.63 pp. 586-594
ISSN:
0161-5890
Subject:
Aeromonas hydrophila; Aphanomyces; B-lymphocytes; Channa striata; Escherichia coli; Laminaria; Takifugu rubripes; amino acids; apoptosis; bacteria; beta-glucans; cDNA libraries; caspases; complementary DNA; fish; fungi; gene expression; gene expression regulation; messenger RNA; nucleotide sequences; phylogeny; polyinosinic-polycytidylic acid; polypeptides; prediction; proteomics; sequence analysis; signal peptide; spleen
Abstract:
... B-cell lymphoma-2 (BCL-2) is a suppressor of apoptosis and inhibits the caspase dependent apoptosis pathway. In this study, we report molecular characterization of a cDNA sequence encoded of BCL-2 from striped murrel, Channa striatus. A partial cDNA sequence of CsBCL-2 was identified from the striped murrel cDNA library during annotation. Subsequently, the full length CsBCL-2 cDNA sequence was obt ...
DOI:
10.1016/j.molimm.2014.07.018

15. A New Arylalkylamine N-Acetyltransferase in Silkworm (Bombyx mori) Affects Integument Pigmentation

Author:
Long, Yaohang; Li, Jiaorong; Zhao, Tianfu; Li, Guannan; Zhu, Yong
Source:
Applied biochemistry and biotechnology 2015 v.175 no.7 pp. 3447-3457
ISSN:
0273-2289
Subject:
Bombyx mori; amino acids; biotechnology; complementary DNA; dopamine; enzyme activity; genes; genetically modified organisms; head; high performance liquid chromatography; integument; melanin; melatonin; metabolism; pigmentation; quantitative polymerase chain reaction; silkworms
Abstract:
... Dopamine is a precursor for melanin synthesis. Arylalkylamine N-acetyltransferase (AANAT) is involved in the melatonin formation in insects because it could catalyze the transformation from dopamine to dopamine-N-acetyldopamine. In this study, we identified a new AANAT gene in the silkworm (Bombyx mori) and assessed its role in the silkworm. The cDNA of this gene encodes 233 amino acids that share ...
DOI:
10.1007/s12010-015-1516-3

16. A new immunoreactive recombinant protein designated as rBoSA2 from Babesia ovis: Its molecular characterization, subcellular localization and antibody recognition by infected sheep

Author:
Sevinc, Ferda; Cao, Shinuo; Zhou, Mo; Sevinc, Mutlu; Ceylan, Onur; Xuan, Xuenan
Source:
Veterinary parasitology 2015 v.214 no.1-2 pp. 213-218
ISSN:
0304-4017
Subject:
Babesia ovis; Escherichia coli; Protozoa; amino acids; antibodies; antigens; babesiosis; blood serum; cDNA libraries; chromatography; complementary DNA; cytoplasm; disease control; enzyme-linked immunosorbent assay; erythrocytes; glutathione transferase; merozoites; messenger RNA; molecular weight; nucleotides; open reading frames; parasites; polypeptides; recombinant fusion proteins; serodiagnosis; sheep
Abstract:
... Ovine babesiosis, caused by the intra-erythrocytic protozoan parasite Babesia ovis, is an infectious and economically important tick-borne disease of sheep. Diagnostic testing is an essential tool used for the control of the disease. In order to identify and characterize the immunoreactive proteins which are useful in serological diagnosis of the disease, a complementary DNA (cDNA) expression libr ...
DOI:
10.1016/j.vetpar.2015.09.022

17. An integrin from oyster Crassostrea gigas mediates the phagocytosis toward Vibrio splendidus through LPS binding activity

Author:
Jia, Zhihao; Zhang, Tao; Jiang, Shuai; Wang, Mengqiang; Cheng, Qi; Sun, Mingzhe; Wang, Lingling; Song, Linsheng
Source:
Developmental and comparative immunology 2015 v.53 no.1 pp. 253-264
ISSN:
0145-305X
Subject:
Crassostrea gigas; Gram-negative bacteria; Gram-positive bacteria; Staphylococcus aureus; Vibrio splendidus; amino acids; apoptosis; binding capacity; cell adhesion; complementary DNA; dissociation; dose response; gene expression; hemocytes; hepatopancreas; integrins; latex; lipopolysaccharides; messenger RNA; muscles; open reading frames; oysters; peptidoglycans; phagocytosis; polyclonal antibodies; polypeptides
Abstract:
... Integrins are a family of cell adhesion molecules which play important roles in the regulation of cell adhesion, migration, proliferation, apoptosis and phagocytosis. In the present study, the immune function of an integrin from the oyster Crassostrea gigas (designated CgIntegrin) was characterized to understand the regulatory mechanism of hemocyte phagocytosis toward different microbes. The full- ...
DOI:
10.1016/j.dci.2015.07.014

18. A NONOVARY‐SPECIFIC VITELLOGENIN RECEPTOR FROM THE ORIENTAL FRUIT FLY, Bactrocera dorsalis (HENDEL)

Author:
Lin, Wei‐Jen; Chien, Ching‐Yi; Tsai, Cheng‐Lung; Chen, Mei‐Er
Source:
Archives of insect biochemistry and physiology 2015 v.90 no.4 pp. 169-180
ISSN:
0739-4462
Subject:
Bactrocera dorsalis; amino acids; autocrine signaling; complementary DNA; embryogenesis; endocytosis; fat body; hemolymph; insects; messenger RNA; oocytes; protein synthesis; reverse transcriptase polymerase chain reaction; vitellogenin
Abstract:
... The yolk protein precursor, vitellogenin (Vg), is absorbed into growing oocytes via receptor‐mediated endocytosis for embryonic development. In this study, a Vg receptor (VgR) cDNA of the oriental fruit fly (Bactrocera dorsalis Hendel) was cloned via RT‐PCR and RACE (GenBank accession no. KR535603) and its expression analyzed. The BdVgR cDNA has a length of 6,585 bp encoding 1,923 amino acids. It ...
DOI:
10.1002/arch.21252

19. A novel antimicrobial peptide derived from fish goose type lysozyme disrupts the membrane of Salmonella enterica

Author:
Kumaresan, Venkatesh; Bhatt, Prasanth; Ganesh, Munuswamy-Ramanujam; Harikrishnan, Ramasamy; Arasu, MariadhasValan; Al-Dhabi, Naif Abdullah; Pasupuleti, Mukesh; Marimuthu, Kasi; Arockiaraj, Jesu
Source:
Molecular Immunology 2015 v.68 pp. 421-433
ISSN:
0161-5890
Subject:
Aeromonas hydrophila; Aphanomyces; Channa striata; Salmonella enterica; Siniperca chuatsi; amino acids; antibiotics; antimicrobial peptides; aquaculture; bacteria; cDNA libraries; cell membranes; cell viability; complementary DNA; flow cytometry; freshwater fish; fungi; geese; gene expression; kidneys; lysozyme; multiple drug resistance; nucleotides; pathogens; scanning electron microscopes; scanning electron microscopy
Abstract:
... In aquaculture, accumulation of antibiotics resulted in development of resistance among bacterial pathogens. Consequently, it became mandatory to find alternative to synthetic antibiotics. Antimicrobial peptides (AMPs) which are described as evolutionary ancient weapons have been considered as promising alternates in recent years. In this study, a novel antimicrobial peptide had been derived from ...
DOI:
10.1016/j.molimm.2015.10.001

20. A novel functional glucose transporter in the white shrimp Litopenaeus vannamei -LvGLUT2- is up-regulated during hypoxia in hepatopancreas

Author:
Martínez-Quintana, José A.; Kikuta, Shingo; Felix-Portillo, Monserrath; Peregrino-Uriarte, Alma B.; Yepiz-Plascencia, Gloria
Source:
Marine environmental research 2015 v.112 pp. 61-67
ISSN:
0141-1136
Subject:
Litopenaeus vannamei; Xenopus; amino acids; cell membranes; complementary DNA; genes; gills; glucose; glucose transporters; hepatopancreas; hypoxia; messenger RNA; metabolism; muscles; oocytes; open reading frames; shrimp
Abstract:
... In hypoxia conditions, the white shrimp Litopenaeus vannamei shifts its energetic metabolism from aerobic to anaerobic, requiring more glucose uptake into the cells by GLUT proteins. We here report a novel glucose transporter in shrimp. The Lvglut2 cDNA is 2473 bp-long containing an ORF of 1458 bp encoding 486 amino acid residues. The deduced protein has the features of a facilitative sugar transp ...
DOI:
10.1016/j.marenvres.2015.09.007

21. A novel gonad-specific Argonaute 4 serves as a defense against transposons in the black tiger shrimp Penaeus monodon

Author:
Leebonoi, Wantana; Sukthaworn, Suchitraporn; Panyim, Sakol; Udomkit, Apinunt
Source:
Fish & shellfish immunology 2015 v.42 no.2 pp. 280-288
ISSN:
1050-4648
Subject:
Gill-associated virus; Penaeus monodon; amino acids; complementary DNA; double-stranded RNA; gene silencing; genes; nucleotides; open reading frames; phylogeny; proteins; retrotransposons; shrimp; testes; translation (genetics); transposons
Abstract:
... Argonaute is a key protein of the small-RNA guided gene regulation process. The Argonaute family is generally divided into two subfamilies; AGO and PIWI. In this study, a cDNA encoding a novel type of Argonaute (PmAgo4) in the black tiger shrimp Penaeus monodon was identified and characterized. PmAgo4 cDNA contained an open reading frame of 2433 nucleotides that can be translated into a deduced am ...
DOI:
10.1016/j.fsi.2014.11.014

22. A novel integrin function in innate immunity from Chinese mitten crab (Eriocheir sinensis)

Author:
Huang, Ying; Zhao, Ling-Ling; Feng, Jin-Ling; Zhu, Huan-Xi; Huang, Xin; Ren, Qian; Wang, Wen
Source:
Developmental and comparative immunology 2015 v.52 no.2 pp. 155-165
ISSN:
0145-305X
Subject:
Aeromonas hydrophila; Bacillus megaterium; Bacillus subtilis; Bacillus thuringiensis; Eriocheir sinensis; Escherichia coli; Gram-negative bacteria; Micrococcus luteus; Staphylococcus aureus; Vibrio anguillarum; Vibrio natriegens; Vibrio parahaemolyticus; agglutination; amino acids; calcium; cell movement; complementary DNA; crabs; epidermal growth factor; gills; heart; hemocytes; hepatopancreas; innate immunity; integrins; intestines; muscles; nerve tissue; open reading frames; receptors; signal peptide; virulence
Abstract:
... Integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. In this study, an integrin called EsIntegrin was identified from Chinese mitten crab Eriocheir sinensis. EsIntegrin cDNA is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. EsIntegrin contains a s ...
DOI:
10.1016/j.dci.2015.05.005

23. A novel laccase from basidiomycete Cerrena sp.: Cloning, heterologous expression, and characterization

Author:
Yang, Jie; Ng, Tzi Bun; Lin, Juan; Ye, Xiuyun
Source:
International journal of biological macromolecules 2015 v.77 pp. 344-349
ISSN:
0141-8130
Subject:
Komagataella pastoris; decolorization; fermentation; recombinant proteins; bioremediation; amino acids; signal peptide; heterologous gene expression; introns; metal ions; industrial applications; complementary DNA; yeasts; laccase; culture media; Cerrena; white-rot fungi; pH; dyes; functional properties
Abstract:
... A novel laccase gene Lac1 and its cDNA were cloned from a white-rot fungus Cerrena sp. and characterized. The 1554-bp cDNA of Lac1 encoded a mature protein with 497 amino acids, preceded by a signal peptide of 20 amino acids. An unconventional intron splice site and incomplete splicing variants of Lac1 were observed. Lac1 was heterologously expressed in the yeast host Pichia pastoris, and a maxima ...
DOI:
10.1016/j.ijbiomac.2015.03.028

24. A novel multi-domain C1qDC protein from Zhikong scallop Chlamys farreri provides new insights into the function of invertebrate C1qDC proteins

Author:
Wang, Leilei; Wang, Lingling; Zhang, Daoxiang; Jiang, Qiufen; Sun, Rui; Wang, Hao; Zhang, Huan; Song, Linsheng
Source:
Developmental and comparative immunology 2015 v.52 no.2 pp. 202-214
ISSN:
0145-305X
Subject:
Azumapecten farreri; Gram-negative bacteria; Gram-positive bacteria; adults; amino acids; apoptosis; beta-glucans; blood serum; complement; complementary DNA; embryogenesis; gene expression regulation; hemocytes; hemolysis; hepatopancreas; humans; hydrophobicity; immunoglobulin G; immunoglobulin M; larvae; ligands; messenger RNA; rabbits; recombinant proteins; scallops; sequence homology; signal peptide; topology; yeasts
Abstract:
... The C1q domain containing (C1qDC) proteins are a family of proteins possessing globular C1q (gC1q) domains, and they rely on this domain to recognize various ligands such as PAMPs, immunoglobulins, ligands on apoptotic cell. In the present study, a novel multi-domain C1qDC protein (CfC1qDC-2) was identified from scallop Chlamys farreri, and its full length cDNA was composed of 1648 bp, encoding a ...
DOI:
10.1016/j.dci.2015.05.009

25. A Novel Soybean (Glycine max) Gene Encoding a Family 3 β-Glucosidase Has High Isoflavone 7-O-Glucoside-Hydrolyzing Activity in Transgenic Rice

Author:
Hsu, Chia-Chen; Wu, Tsung-Meng; Hsu, Yi-Ting; Wu, Chih-Wen; Hong, Chwan-Yang; Su, Nan-Wei
Source:
Journal of agricultural and food chemistry 2015 v.63 no.3 pp. 921-928
ISSN:
0021-8561
Subject:
Glycine max; Oryza sativa; amino acid sequences; amino acids; beta-glucosidase; complementary DNA; conserved sequences; daidzein; daidzin; enzyme activity; genes; genetic engineering; genistein; genistin; hydrolysis; leaves; molecular weight; open reading frames; phylogeny; polypeptides; rice; roots; seeds; sequence analysis; soybeans; substrate specificity; transgenic plants
Abstract:
... A previous study demonstrated that purified Glycine max β-glucosidase (GmBGL) could hydrolyze glucosyl isoflavone to the aglyconic form. This study reports the cloning and functional characterization of a soybean cDNA encoding the β-glucosidase. GmBGL was isolated by use of a purified soybean N-terminal amino acid sequence and conserved sequences of β-glucosidase genes from other plants. Sequen ...
DOI:
10.1021/jf504778x

26. An SCD gene from the Mollusca and its upregulation in carotenoid-enriched scallops

Author:
Li, Xue; Ning, Xianhui; Dou, Jinzhuang; Yu, Qian; Wang, Shuyue; Zhang, Lingling; Wang, Shi; Hu, Xiaoli; Bao, Zhenmin
Source:
Gene 2015 v.564 pp. 101-108
ISSN:
0378-1119
Subject:
stearoyl-CoA desaturase; adults; open reading frames; amino acids; gene expression regulation; introns; tissues; complementary DNA; new variety; muscles; larvae; exons; vitamin A; antioxidants; pigments; diet; aquatic organisms; Mizuhopecten yessoensis; breeding; monounsaturated fatty acids; color; scallops
Abstract:
... Carotenoids are a diverse group of red, orange, and yellow pigments that act as vitamin A precursors and antioxidants. Animals can only obtain carotenoids through their diets. Amongst the carotenoids identified in nature, over one third are of marine origin, but current research on carotenoid absorption in marine species is limited. Bivalves possess an adductor muscle, which is normally white in s ...
DOI:
10.1016/j.gene.2015.02.071

27. A prawn core histone 4: Derivation of N- and C-terminal peptides and their antimicrobial properties, molecular characterization and mRNA transcription

Author:
Chaurasia, Mukesh Kumar; Palanisamy, Rajesh; Bhatt, Prasanth; Kumaresan, Venkatesh; Gnanam, Annie J.; Pasupuleti, Mukesh; Kasi, Marimuthu; Harikrishnan, Ramaswamy; Arockiaraj, Jesu
Source:
Microbiological research 2015 v.170 pp. 78-86
ISSN:
0944-5013
Subject:
Aeromonas hydrophila; Baculoviridae; Gram-negative bacteria; Gram-positive bacteria; Macrobrachium rosenbergii; Vibrio harveyi; amino acids; antibacterial properties; antimicrobial peptides; bioinformatics; cDNA libraries; complementary DNA; gene expression; gills; histones; isoelectric point; messenger RNA; models; molecular weight; monophyly; polypeptides; screening; sequence alignment; shrimp; transcription (genetics); white spot syndrome
Abstract:
... This study investigates the complete molecular characterization including bioinformatics characterization, gene expression, synthesis of N and C terminal peptides and their antimicrobial activity of the core histone 4 (H4) from freshwater giant prawn Macrobrachium rosenbergii (Mr). A cDNA encoding MrH4 was identified from the constructed cDNA library of M. rosenbergii during screening and the sequ ...
DOI:
10.1016/j.micres.2014.08.011

28. A single amino acid substitution in isozyme GST mu in Triclabendazole resistant Fasciola hepatica (Sligo strain) can substantially influence the manifestation of anthelmintic resistance

Author:
Fernández, V.; Estein, S.; Ortiz, P.; Luchessi, P.; Solana, V.; Solana, H.
Source:
Experimental parasitology 2015 v.159 pp. 274-279
ISSN:
0014-4894
Subject:
Fasciola hepatica; RNA; adults; amino acid substitution; amino acids; complementary DNA; drugs; fascioliasis; financial economics; genes; genetic analysis; glutathione; glutathione transferase; helminths; humans; isozymes; reverse transcription; ruminants; triclabendazole
Abstract:
... The helminth parasite Fasciola hepatica causes fascioliasis in human and domestic ruminants. Economic losses due to this infection are estimated in U$S 2000–3000 million yearly. The most common method of control is the use of anthelmintic drugs. However, there is an increased concern about the growing appearance of F. hepatica resistance to Triclabendazole (TCBZ), an anthelmintic with activity ove ...
DOI:
10.1016/j.exppara.2015.10.007

29. A splicing isoform of Ctenopharyngodon idella ADAR1 (CiADAR1-like): Genome organization, tissue specific expression and transcriptional regulation

Author:
Wang, Binhua; Liu, Yong; Sun, Zhicheng; Liu, Xiancheng; Mi, Yichuan; Liu, Dan; Xu, Xiaowen; Hou, Qunhao; Wang, Fang; Hu, Chengyu
Source:
Fish & shellfish immunology 2015 v.47 no.1 pp. 535-544
ISSN:
1050-4648
Subject:
adenosine; adenosine deaminase; Ctenopharyngodon idella; luciferase; phylogeny; Western blotting; gels; Z-DNA; Danio rerio; promoter regions; polypeptides; open reading frames; amino acids; active sites; introns; tissues; Escherichia coli; messenger RNA; alternative splicing; complementary DNA; amino acid sequences; transcription (genetics); double-stranded RNA; kidneys; isozymes; exons; quantitative polymerase chain reaction; deamination; reverse transcriptase polymerase chain reaction; affinity chromatography
Abstract:
... Catalyzing the deamination of adenosine to inosine in RNA, ADAR1 (adenosine deaminase that act on RNA 1) belongs to ADAR family. In our previous work, we have cloned the complete genomic sequence of ADAR1 from grass carp (Ctenopharyngodon idella), named CiADAR1. In the process, we found a splicing isoform of CiADAR1 (CiADAR1-like). CiADAR1 and CiADAR1-like are possessed by different promoters but ...
DOI:
10.1016/j.fsi.2015.09.040

30. Association genetics and expression patterns of a CBF4 homolog in Populus under abiotic stress

Author:
Li, Ying; Xu, Baohua; Du, Qingzhang; Zhang, Deqiang
Source:
Molecular genetics and genomics 2015 v.290 no.3 pp. 913-928
ISSN:
1617-4615
Subject:
Populus simonii; amino acids; cold; cold stress; complementary DNA; drought; gene expression regulation; genes; haplotypes; heat; linkage disequilibrium; marker-assisted selection; phenotypic variation; polypeptides; single nucleotide polymorphism; stress response; stress tolerance
Abstract:
... New strategies for prevention and treatment of abiotic stress require an improved understanding of stress responses. Here, we examined response differences of a C-repeat binding factor gene (PsCBF4) between five species in the genus Populus. We also used a candidate gene-based approach to identify single nucleotide polymorphisms (SNPs) within PsCBF4 that were associated with physiological and bioc ...
DOI:
10.1007/s00438-014-0967-5

31. ATP selection in a random peptide library consisting of prebiotic amino acids

Author:
Kang, Shou-Kai; Chen, Bai-Xue; Tian, Tian; Jia, Xi-Shuai; Chu, Xin-Yi; Liu, Rong; Dong, Peng-Fei; Yang, Qing-Yong; Zhang, Hong-Yu
Source:
Biochemical and biophysical research communications 2015 v.466 pp. 400-405
ISSN:
0006-291X
Subject:
adenosine triphosphate; amino acids; complementary DNA; evolution; high-throughput nucleotide sequencing; models; peptide libraries; prebiotics; proteins
Abstract:
... Based upon many theoretical findings on protein evolution, we proposed a ligand-selection model for the origin of proteins, in which the most ancient proteins originated from ATP selection in a pool of random peptides. To test this ligand-selection model, we constructed a random peptide library consisting of 15 types of prebiotic amino acids and then used cDNA display to perform six rounds of in v ...
DOI:
10.1016/j.bbrc.2015.09.038

32. Biochemical and molecular characterization of the calcineurin in Echinococcus granulosus larval stages

Author:
Nicolao, María Celeste; Cumino, Andrea C.
Source:
Acta tropica 2015 v.146 pp. 141-151
ISSN:
0001-706X
Subject:
Echinococcus granulosus; RNA helicases; amino acids; bladder; calcium; calcium signaling; chelating agents; complementary DNA; confocal microscopy; cyclosporine; cytoplasm; enzyme activity; exocytosis; fluorescence; genes; humans; larvae; parasites; protein subunits; protoscoleces
Abstract:
... Calcineurin (CaN) is a Ca²⁺-calmodulin activated serine–threonine protein phosphatase that couples the local or global calcium signals, thus controlling important cellular functions in physiological and developmental processes. The aim of this study was to characterize CaN in Echinococcus granulosus (Eg-CaN), a human cestode parasite of clinical importance, both functionally and molecularly. We fo ...
DOI:
10.1016/j.actatropica.2015.03.016

33. Biochemical characterization and spatio-temporal expression of myo-inositol oxygenase (MIOX) from wheat (Triticum aestivum L.)

Author:
Alok, Anshu; Kaur, Harsimran; Bhati, Kaushal K.; Kumar, Jitesh; Pandey, Pankaj; Upadhyay, Santosh K.; Pandey, Ashutosh; Sharma, Naresh C.; Pandey, Ajay K.; Tiwari, Siddharth
Source:
Plant gene 2015 v.4 pp. 10-19
ISSN:
2352-4073
Subject:
developmental stages; phylogeny; wheat; genes; catalytic activity; roots; polypeptides; glucuronic acid; stems; open reading frames; aleurone layer; amino acids; Triticum aestivum; oxidation; molecular weight; gene expression regulation; oxygenases; leaves; complementary DNA; myo-inositol; Aegilops tauschii; Triticum urartu; biochemical pathways; endosperm; seed development
Abstract:
... Myo-inositol oxygenase (MIOX) catalyzes the conversion of myo-inositol into d-glucuronic acid. The present study demonstrates isolation of MIOX cDNA (TaMIOX) from wheat (Triticum aestivum L.) with open reading frame of 912bp encoding 303 amino acid polypeptides with a molecular mass of 35.2kDa. Phylogenetic analysis of TaMIOX across kingdoms confirmed the close relationship with Triticum urartu an ...
DOI:
10.1016/j.plgene.2015.09.004

34. Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behavior in response to phytohormones in Picrorhiza kurrooa Royle ex Benth.

Author:
Parkash, Jai; Kashyap, Sanjeeta; Kirti, Shruti; Singh, Anil Kumar; Dutt, Som
Source:
Plant gene 2015 v.3 pp. 11-19
ISSN:
2352-4073
Subject:
Picrorhiza kurrooa; abscisic acid; abscission; amino acids; apoptosis; cathepsin B; complementary DNA; cytokinins; endangered species; gene expression regulation; genes; herbal medicines; isoelectric point; jasmonic acid; leaves; medicinal plants; molecular cloning; molecular weight; open reading frames; pathogens; pests; polymerase chain reaction; protein secondary structure; research and development; ripening; senescence (aging); Himalayan region
Abstract:
... Medicinal importance of Picrorhiza (Picrorhiza kurrooa Royle ex Benth — an herb of western Himalayan region) and its endangered status in Red Data Book presses an urgent need for intensive R&D interventions towards ensuring its availability for the medicinal use, its sustainability and improvement. The present study was conducted on cathepsin B cysteine protease in Picrorhiza. Cathepsin B cysteine ...
DOI:
10.1016/j.plgene.2015.07.001

35. Cathepsin L is an immune-related protein in Pacific abalone (Haliotis discus hannai) – Purification and characterization

Author:
Shen, Jian-Dong; Cai, Qiu-Feng; Yan, Long-Jie; Du, Cui-Hong; Liu, Guang-Ming; Su, Wen-Jin; Ke, Caihuan; Cao, Min-Jie
Source:
Fish & shellfish immunology 2015 v.47 no.2 pp. 986-995
ISSN:
1050-4648
Subject:
EDTA (chelating agent); Haliotis discus hannai; Pinctada fucata; Vibrio parahaemolyticus; Western blotting; abalone; amino acids; ammonium sulfate; cathepsin L; complementary DNA; cysteine proteinase inhibitors; enzyme activity; ethylene glycol tetraacetic acid; gene expression regulation; glycoproteins; glycosylation; hepatopancreas; innate immunity; molecular cloning; molecular weight; open reading frames; oysters; pH; polyacrylamide gel electrophoresis; polymerase chain reaction; temperature
Abstract:
... Cathepsin L, an immune-related protein, was purified from the hepatopancreas of Pacific abalone (Haliotis discus hannai) by ammonium sulfate precipitation and column chromatographies of SP-Sepharose and Sephacryl S-200 HR. Purified cathepsin L appeared as two bands with molecular masses of 28.0 and 28.5 kDa (namely cathepsin La and Lb) on SDS-PAGE under reducing conditions, suggesting that it is a ...
DOI:
10.1016/j.fsi.2015.11.004

36. cDNA-AFLP analysis on 2 Osmanthus fragrans cultivars with different flower color and molecular characteristics of OfMYB1 gene

Author:
Han, Yuanji; Chen, Weicai; Yang, Fengbo; Wang, Xiaohui; Dong, Meifang; Zhou, Ping; Shang, Fude
Source:
Trees 2015 v.29 no.3 pp. 931-940
ISSN:
0931-1890
Subject:
Osmanthus fragrans; amino acids; anthocyanins; biochemical pathways; cell nucleus; color; complementary DNA; corolla; cultivars; gene expression; gene expression regulation; genes; hybrids; phylogeny; polypeptides; protoplasts; sequence analysis; signal transduction; transcription factors; trees; yeasts
Abstract:
... KEY MESSAGE : In this study, we carried out cDNA-AFLP analysis on petals of 2 Osmanthus fragrans cultivars, yielding 102 cDNA fragments. OfMYB1 gene was cloned and the molecular characteristics were studied. The Osmanthus fragrans cultivars ‘Dangui’ and ‘Yingui’ differ considerably in floral coloration and volatiles. We carried out cDNA-AFLP analysis on petals of the two cultivars to isolate genes ...
DOI:
10.1007/s00468-015-1175-6

37. cDNA cloning and characterization of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus venom

Author:
Hatakeyama, Tomomitsu; Ichise, Ayaka; Yonekura, Tomokazu; Unno, Hideaki; Goda, Shuichiro; Nakagawa, Hideyuki
Source:
Toxicon 2015 v.94 pp. 8-15
ISSN:
0041-0101
Subject:
Echinoidea; Escherichia coli; amino acids; carbohydrate binding; chemotaxis; complementary DNA; cytotoxicity; erythrocytes; fish eggs; genes; hemagglutination; lectins; nucleotides; open reading frames; rabbits; reverse transcriptase polymerase chain reaction; rhamnose; signal peptide; transcription (genetics); venoms
Abstract:
... The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contain several biologically active proteins. Among these, a galactose-binding lectin SUL-I isolated from the venom in the large globiferous pedicellariae shows several activities such as mitogenic, chemotactic, and cytotoxic activities through binding to the carbohydrate chains on the cells. We cloned cDNA encoding SUL ...
DOI:
10.1016/j.toxicon.2014.11.236

38. cDNA cloning and expression of Contractin A, a phospholipase A2-like protein from the globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus

Author:
Hatakeyama, Tomomitsu; Higashi, Erika; Nakagawa, Hideyuki
Source:
Toxicon 2015 v.108 pp. 46-52
ISSN:
0041-0101
Subject:
Echinoidea; Escherichia coli; amino acids; complementary DNA; muscle contraction; nucleotide sequences; phospholipase A2; predators; proteins; reverse transcriptase polymerase chain reaction; sequence homology; signal peptide; smooth muscle; toxicity; transcription (genetics)
Abstract:
... Venomous sea urchins contain various biologically active proteins that are toxic to predators. Contractin A is one such protein contained within the globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus. This protein exhibits several biological activities, such as smooth muscle contraction and mitogenic activity. N-terminal amino acid residues of Contractin A have been determi ...
DOI:
10.1016/j.toxicon.2015.09.040

39. cDNA CLONING AND TRANSCRIPTIONAL REGULATION OF THE CECROPIN AND ATTACIN FROM THE ORIENTAL FRUIT FLY, Bactrocera dorsalis (DIPTERA: TEPHRITIDAE)

Author:
Liao, Yin‐Yin; Zuo, Yu‐Han; Tsai, Cheng‐Lung; Hsu, Chia‐Ming; Chen, Mei‐Er
Source:
Archives of insect biochemistry and physiology 2015 v.89 no.2 pp. 111-126
ISSN:
0739-4462
Subject:
Bactrocera dorsalis; adults; amino acids; anti-infective properties; antimicrobial peptides; complementary DNA; fat body; females; fruit trees; gene expression; insect pests; larvae; males; molecular weight; open reading frames; pupae; pupation; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; transcription (genetics)
Abstract:
... We described the cDNA cloning of two antimicrobial peptides (AMPs), cecropin (BdCec), and attacin C (BdAttC), from the oriental fruit fly, Bactrocera dorsalis (Hendel), a serious insect pest of fruit trees. Using rapid amplification of cDNA ends, fragments encompassing the entire open reading frames of BdCec and BdAttC were cloned and sequenced. The complete 425 bp cDNA of BdCec encodes a protein ...
DOI:
10.1002/arch.21230

40. cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei

Author:
Li, Guang-li; Deng, Si-ping; Jiang, Shu-na; Ye, Man; Chen, Hua-pu; Chan, Siuming F.; Zhu, Chun-hua
Source:
Genes & genomics 2015 v.37 no.10 pp. 883-892
ISSN:
1976-9571
Subject:
Litopenaeus vannamei; Marsupenaeus; Penaeus monodon; adults; amino acids; animal ovaries; antibodies; complementary DNA; eggs; females; fluorescence; gene expression; gills; heart; hepatopancreas; intestines; juveniles; males; messenger RNA; muscles; nauplii; open reading frames; polypeptides; quantitative polymerase chain reaction; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; sequence analysis; shrimp; signal peptide; testes
Abstract:
... In this study, the full-length GIH cDNA sequence from Litopenaeus vannamei was cloned from the eyestalk by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The full-length GIH cDNA was 865 bp with a 288 bp open-reading frame, which encoded a 96 amino acid prepro-GIH with 17 amino acid signal peptide. L. vannamei GIH (LvGIH) can be classified as a membe ...
DOI:
10.1007/s13258-015-0315-0

41. cDNA structure and the effect of fasting on myostatin expression in walking catfish (Clarias macrocephalus, Günther 1864)

Author:
Kanjanaworakul, Poonmanee; Srisapoome, Prapansak; Sawatdichaikul, Orathai; Poompuang, Supawadee
Source:
Fish physiology and biochemistry 2015 v.41 no.1 pp. 177-191
ISSN:
0920-1742
Subject:
Clarias batrachus; Clarias macrocephalus; adults; amino acids; brain; complementary DNA; energy conservation; fasting; fish; genes; juveniles; larvae; messenger RNA; muscles; myostatin; open reading frames; proteolysis; skeletal muscle; starvation; transforming growth factor beta
Abstract:
... We cloned and sequenced the myostatin (MSTN) gene of walking catfish and characterized its expression under different conditions. The full cDNA sequence of MSTN was 1,784 bp, containing an open reading frame of 1,191 bp, which encoded 396 amino acids. The deduced MSTN sequence contained functional sites similar to other members of TGF-β superfamily, including the proteolytic processing site and ni ...
DOI:
10.1007/s10695-014-0015-8

42. CgIκB3, the third novel inhibitor of NF-kappa B (IκB) protein, is involved in the immune defense of the Pacific oyster, Crassostrea gigas

Author:
Xu, Fengjiao; Li, Jun; Zhang, Yuehuan; Li, Xiaomei; Zhang, Yang; Xiang, Zhiming; Yu, Ziniu
Source:
Fish & shellfish immunology 2015 v.46 no.2 pp. 648-655
ISSN:
1050-4648
Subject:
Crassostrea gigas; amino acids; bioinformatics; complementary DNA; dose response; gills; heart; immune response; invertebrates; messenger RNA; molecular weight; non-specific serine/threonine protein kinase; open reading frames; phosphorylation; phylogeny; prediction; sequence analysis; signal transduction; transcription factor NF-kappa B; vertebrates
Abstract:
... Inhibitor of NF-κB (IκB), the important regulator of NF-κB/Rel signaling pathway, plays the crucial role in immune response of both vertebrates and invertebrates. Here, a novel homologue of IκB was cloned from Crassostrea gigas, and designated as CgIκB3. The complete CgIκB3 cDNA was 1282 bp in length, including a 942 bp open reading frame (ORF), a 51 bp 5′ UTR and a 289 bp 3′ UTR. The ORF encodes ...
DOI:
10.1016/j.fsi.2015.08.002

43. Chalcone synthase genes from milk thistle (Silybum marianum): isolation and expression analysis

Author:
SANJARI, SEPIDEH; SHOBBAR, ZAHRA SADAT; EBRAHIMI, MOHSEN; HASANLOO, TAHEREH; SADAT-NOORI, SEYED-AHMAD; TIRNAZ, SOODEH
Source:
Journal of genetics 2015 v.94 no.4 pp. 611-617
ISSN:
0022-1333
Subject:
anthocyanins; quantitative polymerase chain reaction; proteins; flowering; biosynthesis; seeds; complementary DNA; corolla; naringenin-chalcone synthase; open reading frames; Silybum marianum; rapid amplification of cDNA ends; amino acid sequences; amino acids; silymarin; exons; leaves
Abstract:
... Silymarin is a flavonoid compound derived from milk thistle (Silybum marianum) seeds which has several pharmacological applications. Chalcone synthase (CHS) is a key enzyme in the biosynthesis of flavonoids; thereby, the identification of CHS encoding genes in milk thistle plant can be of great importance. In the current research, fragments of CHS genes were amplified using degenerate primers base ...
DOI:
10.1007/s12041-015-0560-7

44. Characterisation of a Recombinant Patchoulol Synthase Variant for Biocatalytic Production of Terpenes

Author:
Frister, Thore; Hartwig, Steffen; Alemdar, Semra; Schnatz, Katharina; Thöns, Laura; Scheper, Thomas; Beutel, Sascha
Source:
Applied biochemistry and biotechnology 2015 v.176 no.8 pp. 2185-2201
ISSN:
0273-2289
Subject:
Pogostemon cablin; amino acids; biocatalysts; biosynthesis; carbon; complementary DNA; essential oils; gas chromatography-mass spectrometry; oils; sesquiterpenoids
Abstract:
... The patchoulol synthase (PTS) is a multi-product sesquiterpene synthases which is the central enzyme for biosynthesis of patchouli essential oil in the patchouli plant. Sesquiterpene synthases catalyse the formation of various complex carbon backbones difficult to approach by organic synthesis. Here, we report the characterisation of a recombinant patchoulol synthase complementary DNA (cDNA) varia ...
DOI:
10.1007/s12010-015-1707-y

45. Characterization and Biological Activities of Ocellatin Peptides from the Skin Secretion of the Frog Leptodactylus pustulatus

Author:
Marani, Mariela Mirta; Dourado, Flávio Santos; Quelemes, Patrick Veras; de Araujo, Alyne Rodrigues; Perfeito, Márcia Luana Gomes; Barbosa, Eder Alves; Véras, Leiz Maria Costa; Coelho, Andreia Luísa Rodrigues; Andrade, Etielle Barroso; Eaton, Peter; Longo, João Paulo Figueiró; Azevedo, Ricardo Bentes; Delerue-Matos, Cristina; Leite, José Roberto S. A.
Source:
Journal of natural products 2015 v.78 no.7 pp. 1495-1504
ISSN:
1520-6025
Subject:
Escherichia coli; Klebsiella pneumoniae; Leptodactylus; Salmonella enterica; Staphylococcus aureus; amino acid sequences; amino acids; antimicrobial peptides; antimicrobial properties; complementary DNA; cytotoxicity; erythrocytes; fibroblasts; frogs; high performance liquid chromatography; humans; mass spectrometry; mice; secretion
Abstract:
... Eight new peptides were isolated from the skin secretion of the frog Leptodactylus pustulatus and their amino acid sequences determined by de novo sequencing and by cDNA cloning. Structural similarities between them and other antimicrobial peptides from the skin secretion of Leptodactylus genus frogs were found. Ocellatins-PT1 to -PT5 (25 amino acid residues) are amidated at the C-terminus, while ...
DOI:
10.1021/np500907t

46. Characterization and expression analyses of five interferon regulatory factor transcripts (Irf4a, Irf4b, Irf7, Irf8, Irf10) in Atlantic cod (Gadus morhua)

Author:
Inkpen, Sabrina M.; Hori, Tiago S.; Gamperl, A. Kurt; Nash, Gordon W.; Rise, Matthew L.
Source:
Fish & shellfish immunology 2015 v.44 no.1 pp. 365-381
ISSN:
1050-4648
Subject:
Aeromonas salmonicida; Gadus morhua; amino acids; bacterial infections; carcinogenesis; complementary DNA; fish; genes; immune response; interferon regulatory factors; interferons; phylogeny; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; spleen; temperature; tissues
Abstract:
... The interferon regulatory factor (IRF) family of genes encodes a group of transcription factors that have important roles not only in regulating the expression of Type I interferons (IFNs) and other genes in the IFN pathway, but also in growth, development and the regulation of oncogenesis. In this study, several IRF family members (Irf4a, Irf4b, Irf7, Irf8, Irf10) in Atlantic cod (Gadus morhua) w ...
DOI:
10.1016/j.fsi.2015.02.032

47. Characterization and expression analysis of a gene encoding CBF/DREB1 transcription factor from mangrove Aegiceras corniculatum

Author:
Peng, Ya-Lan; Wang, You-Shao; Cheng, Hao; Wang, Li-Ying
Source:
Ecotoxicology 2015 v.24 no.7-8 pp. 1733-1743
ISSN:
0963-9292
Subject:
Aegiceras corniculatum; Avicennia marina; DNA-binding domains; abscisic acid; amino acids; cold; cold stress; complementary DNA; drought; gene expression; genes; leaves; open reading frames; quantitative polymerase chain reaction; salinity; salt stress; sequence analysis; signal transduction; tissues; transcription factors
Abstract:
... Several transcription factors play important roles in survival of plants under cold, drought and salt stresses by serving as master regulator of sets of downstream stress-responsive genes. A gene encoding CBF/DREB1 transcription factor (C-repeat binding factor/dehydration responsive element-binding factor 1) was isolated from mangrove Aegiceras corniculatum and designated AcCBF1. The full-length c ...
DOI:
10.1007/s10646-015-1485-x

48. Characterization and expression analysis of BCA2 gene in large yellow croaker, Larimichthys crocea

Author:
Zhang, Dong Ling; Yu, Da Hui; Chen, Jian; Wang, Zhi Yong
Source:
Fish & shellfish immunology 2015 v.46 no.2 pp. 543-549
ISSN:
1050-4648
Subject:
Larimichthys crocea; Vibrio; amino acids; bacteria; blood; complementary DNA; fish; genes; heart; in situ hybridization; kidneys; liver; mammals; muscles; open reading frames; parasites; polypeptides; quantitative polymerase chain reaction; spleen; tissues; ubiquitin-protein ligase; viruses
Abstract:
... BCA2, as an E3 ubiquitin ligase, is an important anti-virus immune factor in mammals. Up to date, there are not any related reports on BCA2 protein in fishes yet. In the present investigation, BCA2 in large yellow croaker Larimichthys crocea (named as LcBCA2) was identified and characterized. The full-length cDNA of LcBCA2 was 1571 bp, including an ORF of 888 bp encoding a polypeptide of 295 amino ...
DOI:
10.1016/j.fsi.2015.07.014

49. Characterization and expression analysis of laboratory of genetics and physiology 2 gene in sea perch, Lateolabrax japonicus

Author:
Jia, Peng; Zhang, Jing; Jin, Yilin; Zeng, Lin; Jia, Kuntong; Yi, Meisheng
Source:
Fish & shellfish immunology 2015 v.47 no.1 pp. 214-220
ISSN:
1050-4648
Subject:
Lateolabrax japonicus; amino acids; complementary DNA; cytosol; farmed fish; gene expression; gene expression regulation; genes; kidneys; liver; messenger RNA; nucleotide sequences; perch; polypeptides; receptors; restriction endonucleases; spleen; tissues
Abstract:
... LGP2 (laboratory of genetics and physiology 2) as a key component of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), plays a predominant role in modulating RLRs-mediated cellular antiviral signaling during viral infection. In the present study, we cloned the LGP2 gene from the sea perch (Lateolabrax japonicus) (LjLGP2), an economically important farmed fish. The complete cDNA seq ...
DOI:
10.1016/j.fsi.2015.09.004

50. Characterization and expression analysis of Toll-like receptor 2 gene in large yellow croaker, Larimichthys crocea

Author:
Fan, Ze-Jun; Jia, Qiao-Jing; Yao, Cui-Luan
Source:
Fish & shellfish immunology 2015 v.44 no.1 pp. 129-137
ISSN:
1050-4648
Subject:
Larimichthys crocea; Toll-like receptor 2; Vibrio parahaemolyticus; amino acids; bacterial infections; blood; complementary DNA; cytoplasm; fish; flagellin; genes; innate immunity; kidneys; lipopolysaccharides; liver; open reading frames; peptidoglycans; polypeptides; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; spleen; stomach; tissues
Abstract:
... Toll-like receptor 2 (TLR2) plays an important role in innate immune responses. Here we describe the isolation and characterization of the full-length cDNA sequence of toll-like receptor 2 in large yellow croaker Larimichthys crocea (LcTLR2). The LcTLR2 cDNA contains a 5′-terminal untranslated region (5′-UTR) of 135 bp, an open reading frame (ORF) of 2478 bp encoding a polypeptide of 825 amino aci ...
DOI:
10.1016/j.fsi.2015.01.037