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... BACKGROUND: To determine the effect of flavonoids in conjunction with antibiotics in methicillin resistant Staphylococcus aureus (MRSA) a study was designed. The flavonoids included Rutin, Morin, Qurecetin while antibiotics included ampicillin, amoxicillin, cefixime, ceftriaxone, vancomycin, methicillin, cephradine, erythromycin, imipenem, sulphamethoxazole/trimethoprim, ciprofloxacin and levolflo ...
... In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for t ...
... The objective of this study was to investigate the risks associated with the use of roof-harvested rainwater (RHRW) and the implication of pigeons as the most likely source of contamination by testing for antibiotic resistance profiles of Escherichia coli. A total of 239 E. coli were isolated from thirty fresh pigeon faecal samples (130 isolates), 11 RHRW tanks from three sites in Pretoria (78) an ...
... The antibiotic susceptibility profile of the Bacteroides fragilis group, Gram-positive anaerobic cocci (GPAC), Fusobacterium spp., Prevotella spp., Veillonella spp. and Bilophila wadsworthia for amoxicillin, amoxicillin–clavulanic acid, clindamycin and metronidazole was determined. Human clinical isolates were isolated between 2011 and 2013 at the Microbiological Diagnostic Laboratory of the Unive ...
Salmonella enterica subsp. enterica; amoxicillin; antibiotic resistance; cefotaxime; ceftiofur; cephalothin; chloramphenicol; ciprofloxacin; egg shell; eggs; flocks; genes; gentamicin; industry; multiple drug resistance; neomycin; phenotype; public health; risk; streptomycin; tetracycline; trimethoprim; New South Wales; South Australia
Abstract:
... This study was conducted to examine the antimicrobial resistance (AMR) of Salmonella spp. isolated from commercial caged layer flocks in New South Wales and South Australia. All Salmonella isolates (n=145) were subjected to phenotypic and genotypic characterisation of AMR and carriage of integrons. The majority of Salmonella isolates (91.72%) were susceptible to all antimicrobials tested in this s ...
... In total, 181 streptococci-like bacteria isolated from intramammary infections (IMI) were submitted by a veterinary clinic to Quality Milk Production Services (QMPS, Cornell University, Ithaca, NY). The isolates were characterized by sequence analysis, and 46 Lactococcus lactis ssp. lactis and 47 Lactococcus garvieae were tested for susceptibility to 17 antibiotics. No resistant strains were found ...
... Studies of feline lower urinary tract disease (FLUTD) among Norwegian cats have shown higher prevalences of bacterial cystitis than most previously published reports. The aims of the present study were to identify bacterial isolates obtained from the urine of Norwegian cats with FLUTD and their susceptibility to antimicrobial agents. Eighty-two bacterial isolates from 72 urine cultures obtained fr ...
... The antimicrobial susceptibility of 19 Bordetella avium and 36 Ornithobacterium rhinotracheale strains was tested by the Kirby-Bauer disk diffusion method, and the minimal inhibitory concentrations (MIC) of amoxicillin, doxycycline and erythromycin were also determined. Most O. rhinotracheale strains were resistant to nalidixic acid, sulphamethoxazole–trimethoprim and gentamicin, and were suscepti ...
... Anaerobic bacteria play a significant role in many endogenous polymicrobial infections. Since antimicrobial resistance among anaerobes has increased worldwide, it is useful to provide local susceptibility data to guide empirical therapy. The present study reports recent data on the susceptibility of clinically relevant anaerobes in a University Hospital Centre (UHC) Split, Croatia.A total of 63 Gr ...
... Antibiotic resistance is an emerging global problem. Wild animals are rarely exposed to antibiotics and therefore low levels of antibiotic resistance are expected. However, the growing interactions of these animals with humans and livestock may have a huge impact on their bacterial flora. This study aimed to assess the levels of antibiotic resistance in Escherichia coli isolated from widespread wi ...
... The influence of nitrogen on the interactions between amoxicillin and Microcystis aeruginosa was investigated using a 7-day exposure test. Growth of M. aeruginosa was not significantly (p>0.05) affected by amoxicillin at the lowest nitrogen level of 0.05mgL−1, stimulated by 500ngL−1 of amoxicillin at a moderate nitrogen level of 0.5mgL−1 and enhanced by 200–500ngL−1 of amoxicillin at the highest n ...
... The nucleotide sequence analysis of the gyrB gene indicated that the fish Aeromonas spp. isolates could be identified as Aeromonas hydrophila and Aeromonas veronii biovar sobria, whereas chicken Aeromonas spp. isolates identified as Aeromonas caviae. PCR data revealed the presence of Lip, Ser, Aer, ACT and CAI genes in fish Aer. hydrophila isolates, ACT, CAI and Aer genes in fish Aer. veronii bv s ...
... Acute puerperal metritis (APM) and clinical metritis (CM) are uterine diseases frequently diagnosed in dairy cows. These diseases are responsible for important economic loss because of their effect not only on reproductive performance but also on milk production. The objective of this study was to assess the impact of two different treatments for metritis on dairy cows by measuring their reproduct ...
... This work investigates the competitive adsorption under dynamic and equilibrium conditions of ibuprofen (IBU) and amoxicillin (AMX), two widely consumed pharmaceuticals, on nanoporous carbons of different characteristics. Batch adsorption experiments of pure components in water and their binary mixtures were carried out to measure both adsorption equilibrium and kinetics, and dynamic tests were pe ...
... The aim of this study was to characterise the prevalence of class 1 integrons and gene cassettes, tetracycline-resistance genes, phenicol-resistance genes, 16S rRNA methylase genes, extended-spectrum β-lactamase genes and plasmid-mediated fluoroquinolone resistance determinants in 184 Escherichia coli isolates from chickens in Anhui Province, China. Susceptibility to 15 antimicrobials was determin ...
Salmonella; acetonitrile; amoxicillin; antibiotic residues; centrifugation; chlortetracycline; colistin; detection limit; difloxacin; doxycycline; enrofloxacin; excreta; filtration; flumequine; human diseases; in vivo studies; laying hens; liquid chromatography; penicillin V; poultry products; risk; storage quality; sulfadiazine; tandem mass spectrometry; trimethoprim; ultrasonic treatment
Abstract:
... The most important source of Salmonella spp. infection in humans is by the consumption of contaminated poultry products. Due to the risk of resistance development and its transfer from animals to humans, the Belgian Royal Decree concerning the eradication of Salmonella (C-2007/22784) prohibits treatment of poultry with antimicrobials against zoonotic Salmonella spp. To uncover illicit use, an anal ...
... In this work, a rapid HPLC–DAD method has been developed for the analysis of six antibiotics (amoxicillin, metronidazole, sulfamethoxazole, ofloxacine, sulfadiazine and sulfamerazine) in the sewage treatment plant influent and effluent samples. Decreasing the chromatographic run time to less than 4min as well as lowering the cost per analysis, were achieved through direct injection of the samples ...
Elayaraja Kolanthai; V. Sivaraj Dikeshwar Colon; P. Abinaya Sindu; V. Sarath Chandra; K. R. Karthikeyan; M. Surendar Babu; S. Meenakshi Sundaram; M. Palanichamy; S. Narayana Kalkura
... Tissue engineering deals with the regeneration of tissues for bone repair, wound healing, drug delivery, etc., and a highly porous 3D artificial scaffold is required to accommodate the cells and direct their growth. We prepared 3D porous calcium phosphate ((hydroxyapatite/β-tricalcium phosphate)/agarose, (HAp/β-TCP)/agarose) composite scaffolds by sol–gel technique with water (WBS) and ethanol (EB ...
... The effect of ampicillin (AMP), amoxicillin (AMX), cephalotin (CEP), ciprofloxacin (CPF), gentamycin (GEN), and vancomycin (VAN) have been examined individually and as binary mixtures, on a non-target aquatic organism, the green alga Pseudokichneriella subcapitata. The β-lactam antibiotics AMP and AMX were not toxic to the alga at concentrations up to 2000mgl−1 (less than 10% of algal growth inhib ...
... OBJECTIVES: To investigate whether administration of postoperative oral antimicrobial drugs has a beneficial effect on preventing surgical site infections (SSI) in clean orthopedic surgery involving stainless steel plating systems. STUDY DESIGN: Randomized prospective clinical study. ANIMALS: Dogs (n = 97). METHODS: One hundred consecutive cases (97 dogs) that had clean orthopedic surgery requirin ...
... The effects of medicine on the biomolecular interaction have been given increasing attention in biochemistry and affinity-based analytics since the environment in vivo is complex especially for the patients. Herein, myoglobin, a biomarker of acute myocardial infarction, was used as a model, and the medicine effects on the interactions of myoglobin/aptamer and myoglobin/antibody were systematically ...
Bacteroides; Fusobacterium; Prevotella; amoxicillin; antibiotic resistance; clindamycin; imipenem; matrix-assisted laser desorption-ionization mass spectrometry; metronidazole; multiple drug resistance; neoplasms; patients; species identification; Russia
Abstract:
... In total 122 non-duplicate Bacteroides, Prevotella and Fusobacterium spp isolated from cancer patients between 2004 and 2014 were involved in this study. Most of the strains belonged to the B. fragilis group (55%), followed by Prevotella strains (34.4%) and Fusobacterium spp (10.6%). The species identification was carried out by matrix-assisted laser desorption ionization-time of flight mass spect ...
... The aim of this study was to evaluate a 3-min assay (Betastar® Combo 3.0) for the detection of Penicillin G, Amoxicillin, Cefazolin and Oxytetracycline hydrochloride in individual ewe milk. Antibiotics-free samples were obtained throughout three trials from three Sarda ewes. At the laboratory, samples were spiked with the four antimicrobial molecules at different concentrations. These were on the ...
... The degradation of two organic pollutants (amoxicillin and diclofenac) in 0.1 mM aqueous solutions was studied by using advanced oxidation processes, namely ozonation, photolysis, photolytic ozonation, photocatalysis and photocatalytic ozonation. Diclofenac was degraded quickly under direct photolysis by artificial light (medium-pressure vapor arc, λexc > 300 nm), while amoxicillin remained very s ...
... BACKGROUND: Propionibacterium acnes has been rarely isolated as a commensal from dogs, but there is little evidence of pathogenicity. Urinary tract infections are common in dogs and are typically caused by various commensal bacteria. Here we present the first case report of a urinary tract infection caused by P. acnes. CASE PRESENTATION: A 6-year-old female Japanese Shiba Inu was hospitalized for ...
... The epidemiology of multidrug-resistant bacteria (MDRB) has changed significantly in European healthcare settings, with a decrease in frequency of meticillin-resistant Staphylococcus aureus and an increase in extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. Little is known about the effects of these changes on ventilator-associated pneumonia (VAP). A retrospective 5-year trend an ...
X-ray diffraction; acetaminophen; amoxicillin; biphenyl; ceftriaxone; chemical reactions; chemical structure; detection limit; diclofenac; electrospray ionization mass spectrometry; fluorescence; fluorescent dyes; nuclear magnetic resonance spectroscopy; pH; regression analysis; triazoles
Abstract:
... Novel supramolecular molecular tweezers based on a biphenyl bis-triazole hexahydroquinoline system are designed and synthesized as a highly sensitive and selective fluorescent probe for recognizing and detecting cephradine in the presence of cefotaxime, amoxicillin, penicillin, 6-aminopenicillanic acid, diclofenac, pefloxacin, ceftriaxone, cefaclor and paracetamol at a pH of 7.7. The detection lim ...
... Salmonellosis is an important disease frequently associated with diarrhea in calves. From January to September 2009, a cross-sectional study involving 91 dairy farms was conducted to determine the prevalence of Salmonella spp. infection in cattle in Al-Dhulail Valley, Jordan. A total of 910 calve and cow fecal samples were collected. Information on farm management practices was obtained through pe ...
amoxicillin; antibiotic residues; cefoperazone; ceftiofur; chromatography; detection limit; dicloxacillin; formic acid; liquid-liquid extraction; mass spectrometry; maximum residue limits; methanol; milk; nafcillin; penicillin V; temperature
Abstract:
... This study describes the development and validation procedures for scope extension of a method for the determination of β-lactam antibiotic residues (ampicillin, amoxicillin, penicillin G, penicillin V, oxacillin, cloxacillin, dicloxacillin, nafcillin, ceftiofur, cefquinome, cefoperazone, cephapirine, cefalexin and cephalonium) in bovine milk. Sample preparation was performed by liquid-liquid extr ...
... Coexisting antibiotic contaminants have potential to regulate cyanobacterial bloom, and the regulation is likely affected by nitrogen supply. A typical cyanobaterium Microcystis aeruginosa was cultured with 0.05–50 mg L⁻¹of nitrogen and exposed to 100–600 ng L⁻¹of amoxicillin for 7 days. Algal growth was not significantly (p > 0.05) affected by amoxicillin at the lowest nitrogen level of 0.05 mg L ...
Treponema medium; amoxicillin; antibiotic resistance; cattle; dermatitis; in vivo studies; lameness; lincomycin; minimum inhibitory concentration; oxytetracycline; sheep; sheep industry; spectinomycin; tylosin; veterinary medicine; Ireland; United Kingdom
Abstract:
... BACKGROUND: Contagious ovine digital dermatitis (CODD) is an important cause of infectious lameness in sheep in the UK and Ireland and has a severe impact on the welfare of affected individuals. The three treponemal phylogroups Treponema medium/Treponema vincentii‐like, Treponema phagedenis‐like and Treponema pedis spirochaetes have been associated with clinical CODD lesions and are considered to ...
... Microcystis aeruginosa was cultured with 0.05–5mgL−1 of phosphorus and exposed to 200–500ngL−1 of amoxicillin for seven days. Amoxicillin presented no significant effect (p>0.05) on the growth of M. aeruginosa at phosphorus levels of 0.05 and 0.2mgL−1, but stimulated algal growth as a hormesis effect at phosphorus levels of 1 and 5mgL−1. Phosphorus and amoxicillin affected the contents of chloroph ...
... Contamination of surface waters in underdeveloped countries is a great concern. Treated and untreated wastewaters have been discharged into rivers and streams, leading to possible waterborne infection outbreaks which may represent a significant dissemination mechanism of antibiotic resistance genes among pathogenic bacterial populations.The present study aims to determine the multi-drug resistance ...
World Health Organization; amoxicillin; aqueous solutions; computer software; iodates; iodine; mixing; titration
Abstract:
... A paper test card has been engineered to perform an iodometric titration, an application that requires storage and mixing on demand of several mutually incompatible reagents. The titration is activated when a user applies a test solution to the test card: the dried reagents are reconstituted and combined through a surface-tension-enabled mixing (STEM) mechanism. The device quantifies 0.8–15 ppm of ...
... Only a few studies have evaluated Helicobacter pylori susceptibility to linezolid. The aim of the present study was to assess linezolid susceptibility in H. pylori, including strains with double/multidrug resistance. The susceptibility of 53 H. pylori strains was evaluated by Etest and a breakpoint susceptibility testing method. Helicobacter pylori resistance rates were as follows: amoxicillin, 1. ...
Marta Fernández-Martínez; Elisenda Miró; Adriana Ortega; Germán Bou; Juan José González-López; Antonio Oliver; Alvaro Pascual; Emilia Cercenado; Jesús Oteo; Luis Martínez-Martínez; Ferran Navarro
... The activity of eight aminoglycosides (amikacin, apramycin, arbekacin, gentamicin, kanamycin, neomycin, netilmicin and tobramycin) against a collection of 257 amoxicillin/clavulanic acid (AMC)-resistant Escherichia coli isolates was determined by microdilution. Aminoglycoside resistance rates, the prevalence of aminoglycoside-modifying enzyme (AME) genes, the relationship between AME gene detectio ...
... Helicobacter pylori is a Gram-negative bacterium that colonizes the gut of over 50% of the world’s population. It is responsible for most peptic ulcers and is an important risk factor for gastric cancer. Antibiotic treatment for H. pylori infections is challenging as drug resistance has developed to antibiotics with traditional mechanisms of action. H. pylori uses an unusual pathway for menaquinon ...
amoxicillin; derivatization; desorption; deuterium; drugs; glass; hydroxylation; liquid chromatography; mass spectrometry; metabolites; moieties; nuclear magnetic resonance spectroscopy; polytetrafluoroethylene
Abstract:
... Structural elucidation of metabolites is an important part during the discovery and development process of new pharmaceutical drugs. Liquid Chromatography (LC) in combination with Mass Spectrometry (MS) is usually the technique of choice for structural identification but cannot always provide precise structural identification of the studied metabolite (e.g. site of hydroxylation and site of glucur ...
... The retention of four antibiotics, ciprofloxacin, ofloxacin, amoxicillin and sulfamethoxazole by a natural phosphate rock (francolite) was studied and compared with a converted hydroxyapatite powder. The maximum sorption capacities were found to correlate with the molecular weight of the molecules. The mechanisms of sorption depended mostly on the charge of the antibiotic whereas the kinetics of t ...
... An environmental risk assessment was performed for pharmaceutical compounds present in the aquatic environment of China. Predicted environmental concentration (PEC) of the compounds were calculated according to European Medicines Evaluation Agency (EMEA) guidelines. Available ecotoxicological data compromised by applying a very conservative assessment factor (AF) were employed to calculate the pre ...
Mannheimia haemolytica; Monte Carlo method; Pasteurella multocida; amoxicillin; anti-infective properties; blood serum; calves; equations; intramuscular injection; minimum inhibitory concentration; models; pathogens; pharmacokinetics; plate count; respiratory system
Abstract:
... The antimicrobial properties of amoxicillin were determined for the bovine respiratory tract pathogens, Mannheima haemolytica and Pasteurella multocida. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time‐kill curves were established. Pharmacokinetic (PK)/pharmacodynamic (PD) modelling of the time‐kill data, based on the sigmoidal Eₘₐₓ equation, generated para ...
... Chicken infected with caecal coccidiosis (Eimeria tenella) was used to evaluate the effect of coccidiosis on the pharmacokinetic and bioavailability of amoxicillin. The level of amoxicillin was estimated by high‐performance chromatography (HPLC) to calculate the pharmacokinetic parameters and oral bioavailability. For i.v. injection of amoxicillin, Vd and CL were 0.29 and 0.27 (mg/kg)/(μg/mL)/h, r ...
... Arcobacter butzleri and A. cryaerophilus are considered potential foodborne pathogens. Consumption of Arcobacter-contaminated food is regarded the most likely source of human poisoning. We investigated the prevalence and antimicrobial resistance of Arcobacter isolates in 210 retail meat samples. Seventy-nine A. butzleri and 6 A. cryaerophilus were isolated from pork, beef and chicken meat. Inciden ...
... The aims of this study were to investigate the prevalence and to characterize and determine the antibiotic resistance of Yersinia spp. isolates from raw milk. From September 2008 to August 2010, 446 raw milk samples were obtained from farm bulk milk tanks in Varamin, Iran. Yersinia spp. were detected in 29 (6.5%) samples, out of which 23 (79.3%), 5 (17.2%), and 1 (3.4%) were isolated from cow, she ...
... Increasing evidence suggests that early nutrition has programming effects on adult health. Identifying mechanisms underlying nutritional programming would aid in the design of new disease prevention strategies. The intestinal microbiota could be a key player in this programming because it affects host metabolic homeostasis, postnatal gut colonization is sensitive to early nutrition, and initial mi ...
... The important number of active pharmaceutical ingredients (API) available on the market along with their potential adverse effects in the aquatic ecosystems, lead to the development of prioritization methods, which allow choosing priority molecules to monitor based on a set of selected criteria. Due to the large volumes of API used in hospitals, an increasing attention has been recently paid to th ...
... Extraintestinal pathogenic Escherichia coli (E. coli) is considered as the main causative agent of urinary tract infections worldwide. The relationship between antimicrobial resistance, phylogenetic groups, patient characteristics and adhesin virulence genes are complex and not fully understood. In this study, among 146 urinary isolates of E. coli, phylogenetic groups and various adhesin virulence ...
... A novel separation technique based on an aqueous surfactant extraction to remove organic contaminants from aqueous solutions was investigated. A model was developed regarding the kinetic partitioning of amoxicillin and regarding the mechanism governing the forward transfer of amoxicillin in a reverse micelle system. Results were interpreted in terms of a two-film theory for flat interface. To conf ...
... Two antibacterials, amoxicillin trihydrate and cefuroxime axetil spiked into wastewater were completely removed by sequential wastewater treatment plant's membranes, which included activated sludge, ultrafiltration (hollow fibre and spiral wound membranes with 100 and 20 kDa cut-offs), activated carbon column and reverse osmosis. Adsorption isotherms in synthetic water which employed activated car ...
... The possibility of removing certain pharmaceuticals (acetaminophen and naproxen) from water was tested using Octolig, a commercially available material with polyethylenediimine moieties covalently attached to high-surface area silica gel. In addition, the efficacy of two transition metals (cupric and ferric) derivatives of Octolig was tested. Previously amoxicillin had been successfully subjected ...