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201. Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution

Author:
Schücker, Katharina; Holm, Thorge; Franke, Christian; Sauer, Markus; Benavente, Ricardo
Source:
Proceedings of the National Academy of Sciences of the United States of America 2015 v.112 no.7 pp. 2029-2033
ISSN:
0027-8424
Subject:
chromosomes,   etc   ; image analysis; mammals; microscopy; synaptonemal complex;     Show all 5 Subjects
Abstract:
... Significance Synaptonemal complexes (SCs) are meiosis-specific, 200-nm-wide, ladder-like structures that are essential for synapsis, recombination, and segregation of homologous chromosomes. Despite its importance for the spatial organization of individual components during meiosis, the molecular architecture of the SC is still unknown. We used super-resolution imaging by direct stochastic optical ...
DOI:
10.1073/pnas.1414814112
PubMed:
25646409
PubMed Central:
PMC4343094

202. Improvement of the agronomic traits of a wheat–barley centric fusion by introgressing the 3HS.3BL translocation into a modern wheat cultivar

Author:
Türkösi, Edina; Farkas, András; Aranyi, Nikolett Réka; Hoffmann, Borbála; Tóth, Viola; Molnár-Láng, Márta
Source:
Genome 2015 v.57 no.11/12 pp. 601-607
ISSN:
1480-3321
Subject:
chromosomes,   etc   ; DNA probes; Robertsonian translocation; agronomic traits; alleles; barley; cultivars; dwarfing; field experimentation; fluorescence in situ hybridization; homozygosity; hybrids; introgression; seeds; tillering; wheat; yield components; Hungary;     Show all 18 Subjects
Abstract:
... The 3HS.3BL spontaneous Robertsonian translocation obtained from the progenies of wheat–barley (Chinese Spring × Betzes) hybrids backcrossed with wheat line Mv9kr1 was transferred into the modern Martonvásár wheat cultivar Mv Bodri. The translocation was identified with molecular cytogenetic methods. The inheritance of the translocation was traced using genomic in situ hybridization. Fluorescence ...
DOI:
10.1139/gen-2014-0187

203. Pervasive and Essential Roles of the Top3-Rmi1 Decatenase Orchestrate Recombination and Facilitate Chromosome Segregation in Meiosis

Author:
Tang, Shangming; Wu, Michelle Ka Yan; Zhang, Ruoxi; Hunter, Neil
Source:
Molecular cell 2015 v.57 pp. 607-621
ISSN:
1097-2765
Subject:
chromosome segregation; chromosomes,   etc   ; anaphase; meiosis; yeasts;     Show all 5 Subjects
Abstract:
... The Bloom’s helicase ortholog, Sgs1, plays central roles to coordinate the formation and resolution of joint molecule intermediates (JMs) during meiotic recombination in budding yeast. Sgs1 can associate with type-I topoisomerase Top3 and its accessory factor Rmi1 to form a conserved complex best known for its unique ability to decatenate double-Holliday junctions. Contrary to expectations, we sho ...
DOI:
10.1016/j.molcel.2015.01.021
PubMed:
25699709
PubMed Central:
PMC4791043

204. TOPping Off Meiosis

Author:
Haber, James E.
Source:
Molecular cell 2015 v.57 pp. 577-581
ISSN:
1097-2765
Subject:
chromosomes,   etc   ; homologous recombination; meiosis; proteins; yeasts;     Show all 5 Subjects
Abstract:
... Double-strand breaks (DSBs) threaten chromosome integrity. The most accurate repair of DSBs is by homologous recombination (HR), catalyzed by recombination proteins such as Rad51. Three papers in this issue of Molecular Cell (Fasching et al., 2015; Kaur et al., 2015; Tang et al., 2015) now reveal the role of three of these proteins in budding yeast: Sgs1 (BLM homolog), Top3 (TOPIIIα homolog), and ...
DOI:
10.1016/j.molcel.2015.02.004
PubMed:
25699706
PubMed Central:
PMC4352088

205. Concerted copy number variation balances ribosomal DNA dosage in human and mouse genomes

Author:
Gibbons, John G.; Branco, Alan T.; Godinho, Susana A.; Yu, Shoukai; Lemos, Bernardo
Source:
Proceedings of the National Academy of Sciences of the United States of America 2015 v.112 no.8 pp. 2485-2490
ISSN:
0027-8424
Subject:
chromosomes,   etc   ; bisphenol A; cell nucleolus; equipment; genome; human diseases; humans; mice; natural selection; nucleotide sequences; proteins; ribosomal DNA; ribosomal RNA; ribosomes;     Show all 14 Subjects
Abstract:
... Tandemly repeated ribosomal DNA (rDNA) arrays are among the most evolutionary dynamic loci of eukaryotic genomes. The loci code for essential cellular components, yet exhibit extensive copy number (CN) variation within and between species. CN might be partly determined by the requirement of dosage balance between the 5S and 45S rDNA arrays. The arrays are nonhomologous, physically unlinked in mamm ...
DOI:
10.1073/pnas.1416878112
PubMed:
25583482
PubMed Central:
PMC4345604

206. Analysis of the Complete Mycoplasma hominis LBD-4 Genome Sequence Reveals Strain-Variable Prophage Insertion and Distinctive Repeat-Containing Surface Protein Arrangements

Author:
Calcutt, Michael J.; Foecking, Mark F.
Source:
Genome Announcements 2015 v.3 no.1 pp. e01582-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Mycoplasma arthritidis; Mycoplasma fermentans; Mycoplasma hominis; genes; nucleotide sequences; sequence analysis; surface proteins;     Show all 8 Subjects
Abstract:
... The complete genome sequence of Mycoplasma hominis LBD-4 has been determined and the gene content ascribed. The 715,165-bp chromosome contains 620 genes, including 14 carried by a strain-variable prophage genome related to Mycoplasma fermentans MFV-1 and Mycoplasma arthritidis MAV-1. Comparative analysis with the genome of M. hominis PG21 ᵀ reveals distinctive arrangements of repeat-containing sur ...
DOI:
10.1128/genomeA.01582-14

207. Bifidobacterium pseudolongum Strain PV8-2, Isolated from a Stool Sample of an Anemic Kenyan Infant

Author:
Vazquez-Gutierrez, Pamela; Lacroix, Christophe; Chassard, Christophe; Klumpp, Jochen; Stevens, Marc J. A.; Jans, Christoph
Source:
Genome Announcements 2015 v.3 no.1 pp. e01469-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Bifidobacterium pseudolongum; anemia; feces; genome; nucleotide sequences; plasmids;     Show all 7 Subjects
Abstract:
... The complete genome sequence of Bifidobacterium pseudolongum PV8-2, isolated from feces of an anemic Kenyan infant, was determined using single-molecule real-time (SMRT) technology. The genome consists of a 2-Mbp chromosome and a 4-kb plasmid. ...
DOI:
10.1128/genomeA.01469-14

208. Complete Genome Sequence of an Aldoxime Degrader, Bacillus sp. OxB-1

Author:
Yamaguchi, Takuya; Asano, Yasuhisa
Source:
Genome Announcements 2015 v.3 no.1 pp. e00025-15
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Bacillus (bacteria); nucleotide sequences;     Show all 3 Subjects
Abstract:
... Bacillus sp. OxB-1 has been characterized as a strain that produces a new enzyme, aldoxime dehydratase, which catalyzes the dehydration of aldoxime to form nitrile. Here, its complete genome sequence (3,594,618 bp, with a GC content of 47.85%), comprising a circular chromosome, is announced. ...
DOI:
10.1128/genomeA.00025-15

209. Complete Genome Sequence of Mycobacterium sp. Strain VKM Ac-1817D, Capable of Producing 9α-Hydroxy-androst-4-ene-3,17-dione from Phytosterol

Author:
Shtratnikova, Victoriya Y.; Schelkunov, Mikhail I.; Dovbnya, Dmitry V.; Pekov, Yury A.; Bragin, Eugeny Y.; Ashapkin, Vasily V.; Donova, Marina V.
Source:
Genome Announcements 2015 v.3 no.1 pp. e01447-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Mycobacterium; genome; nucleotide sequences; pharmaceutical industry; phytosterols; ribosomal RNA; transfer RNA;     Show all 8 Subjects
Abstract:
... Mycobacterium sp. strain VKM Ac-1817D is capable of converting phytosterol into 9α-hydroxy androst-4-ene-3,17-dione (9-OH-AD), which is a valuable intermediate for the steroid pharmaceutical industry. Here, a complete genome sequence of the strain is reported. The genome consists of a single circular 6,324,222-bp chromosome with a G+C content of 66.2% and encodes approximately 6,000 CDSs, 54 tRNAs ...
DOI:
10.1128/genomeA.01447-14

210. Complete Genome Sequence of Yersinia ruckeri Strain CSF007-82, Etiologic Agent of Red Mouth Disease in Salmonid Fish

Author:
Nelson, Michael C.; LaPatra, Scott E.; Welch, Timothy J.; Graf, Joerg
Source:
Genome Announcements 2015 v.3 no.1 pp. e01491-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Salmonidae; Yersinia ruckeri; etiology; mouth; nucleotide sequences; operon; transfer RNA;     Show all 8 Subjects
Abstract:
... We present the complete, closed, and finished chromosomal and extrachromosomal genome sequences of Yersinia ruckeri strain CSF007-82, the etiologic agent of enteric red mouth disease in salmonid fish. The chromosome is 3,799,036 bp with a G+C content of 47.5% and encodes 3,530 predicted coding sequences (CDS), 7 ribosomal operons, and 80 tRNAs. ...
DOI:
10.1128/genomeA.01491-14

211. Finished Genome Sequence of Bacillus cereus Strain 03BB87, a Clinical Isolate with B. anthracis Virulence Genes

Author:
Johnson, Shannon L.; Minogue, Timothy D.; Teshima, Hazuki; Davenport, Karen W.; Shea, April A.; Miner, Haven L.; Wolcott, Mark J.; Chain, Patrick S. G.
Source:
Genome Announcements 2015 v.3 no.1 pp. e01446-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Bacillus cereus; blood; genes; males; nucleotide sequences; pathogens; plasmids; pneumonia; virulence;     Show all 10 Subjects
Abstract:
... Bacillus cereus strain 03BB87, a blood culture isolate, originated in a 56-year-old male muller operator with a fatal case of pneumonia in 2003. Here we present the finished genome sequence of that pathogen, including a 5.46-Mb chromosome and two plasmids (209 and 52 Kb, respectively). ...
DOI:
10.1128/genomeA.01446-14

212. Finished Genome Sequence of Collimonas arenae Cal35

Author:
Wu, Je-Jia; de Jager, Victor C. L.; Deng, Wen-Ling; Leveau, Johan H. J.
Source:
Genome Announcements 2015 v.3 no.1 pp. e01408-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; forest soils; genome; nucleotide sequences; plasmids;     Show all 5 Subjects
Abstract:
... We announce the finished genome sequence of soil forest isolate Collimonas arenae Cal35, which comprises a 5.6-Mbp chromosome and 41-kb plasmid. The Cal35 genome is the second one published for the bacterial genus Collimonas and represents the first opportunity for high-resolution comparison of genome content and synteny among collimonads. ...
DOI:
10.1128/genomeA.01408-14

213. Genome Sequence of Borrelia chilensis VA1, a South American Member of the Lyme Borreliosis Group

Author:
Huang, Weihua; Ojaimi, Caroline; Fallon, John T.; Travisany, Dante; Maass, Alejandro; Ivanova, Larisa; Tomova, Alexandra; González-Acuáña, Daniel; Godfrey, Henry P.; Cabello, Felipe C.
Source:
Genome Announcements 2015 v.3 no.1 pp. e01535-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Borrelia burgdorferi; Lyme disease; nucleotide sequences; pathogenicity; plasmids; sequence analysis; Chile;     Show all 8 Subjects
Abstract:
... Borrelia chilensis strain VA1 is a recently described South American member of the Borrelia burgdorferi sensu lato complex from Chile. Whole-genome sequencing analysis determined its linear chromosome and plasmids lp54 and cp26, confirmed its membership in the Lyme borreliosis group, and will open new research avenues regarding its pathogenic potential. ...
DOI:
10.1128/genomeA.01535-14

214. Genome Sequence of Bradyrhizobium japonicum E109, One of the Most Agronomically Used Nitrogen-Fixing Rhizobacteria in Argentina

Author:
Torres, Daniela; Revale, Santiago; Obando, Melissa; Maroniche, Guillermo; Paris, Gastón; Perticari, Alejandro; Vazquez, Martóín; Wisniewski-Dyóíé, Florence; Martóíéínez-Abarca, Francisco; Cassóíéíán, Fabricio
Source:
Genome Announcements 2015 v.3 no.1 pp. e01566-14
ISSN:
2169-8287
Subject:
chromosomes,   etc   ; Bradyrhizobium japonicum; biosynthesis; genes; nitrogen fixation; nucleotide sequences; plant hormones; rhizosphere bacteria; soybeans; Argentina;     Show all 10 Subjects
Abstract:
... We present here the complete genome sequence of Bradyrhizobium japonicum strain E109, one of the most used rhizobacteria for soybean inoculation in Argentina since the 1970s. The genome consists of a 9.22-Mbp single chromosome and contains several genes related to nitrogen fixation, phytohormone biosynthesis, and a rhizospheric lifestyle. ...
DOI:
10.1128/genomeA.01566-14

215. Choreography of the Mycobacterium Replication Machinery during the Cell Cycle

Author:
Trojanowski, Damian; Ginda, Katarzyna; Pióro, Monika; Hołóówka, Joanna; Skut, Partycja; Jakimowicz, Dagmara; Zakrzewska-Czerwióóńska, Jolanta
Source:
mBio 2015 v.6 no.1 pp. e02125-14
ISSN:
2150-7511
Subject:
chromosome segregation; chromosomes,   etc   ; Escherichia coli; Mycobacterium smegmatis; bacteria; fluorescence microscopy;     Show all 6 Subjects
Abstract:
... It has recently been demonstrated that bacterial chromosomes are highly organized, with specific positioning of the replication initiation region. Moreover, the positioning of the replication machinery (replisome) has been shown to be variable and dependent on species-specific cell cycle features. Here, we analyzed replisome positions in Mycobacterium smegmatis , a slow-growing bacterium that exhi ...
DOI:
10.1128/mBio.02125-14
PubMed:
25691599
PubMed Central:
PMC4337567

216. A mutagenicity and cytotoxicity study on Limonium effusum aqueous extracts by Allium, Ames and MTT tests

Author:
Eren, Y.; Özata, A.; Konuk, M.; Akyil, D.; Liman, R.
Source:
Cytology and genetics 2015 v.49 no.2 pp. 125-133
ISSN:
0095-4527
Subject:
chromosome aberrations; chromosomes,   etc   ; Allium; Ames test; Limonium; Salmonella Typhimurium; alternative medicine; carcinogenicity; cytotoxicity; dose response; growth retardation; indigenous species; leaves; mammals; mutagenicity; plant extracts; root growth; teratogenicity;     Show all 18 Subjects
Abstract:
... Nowadays plants or plant extracts have become very important for alternative medicine. Plants and their extracts have many therapeutical advantages but some of them are potentially toxic, mutagenic, carcinogenic and teratogenic. Root, stem and leaf parts of Limonium effusum were used in this study and this species is an endemic species for Turkey. Mutagenic and cytotoxic effects of root, stem and ...
DOI:
10.3103/S0095452715020024

217. Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori

Author:
Mitsudome, Takumi; Mon, Hiroaki; Xu, Jian; Li, Zhiqing; Lee, Jae Man; Patil, Anandrao Ashok; Masuda, Atsushi; Iiyama, Kazuhiro; Morokuma, Daisuke; Kusakabe, Takahiro
Source:
Insect biochemistry and molecular biology 2015 v.58 pp. 55-65
ISSN:
0965-1748
Subject:
chromosomes,   etc   ; Bombyx mori; DNA; DNA methylation; S-adenosylmethionine; epigenetics; gene expression; genome; invertebrates; mammals; manganese; metal ions; methyltransferases; silkworms; substrate specificity; zinc;     Show all 16 Subjects
Abstract:
... DNA methylation is an important epigenetic mechanism involved in gene expression of vertebrates and invertebrates. In general, DNA methylation profile is established by de novo DNA methyltransferases (DNMT-3A, -3B) and maintainance DNA methyltransferase (DNMT-1). DNMT-1 has a strong substrate preference for hemimethylated DNA over the unmethylated one. Because the silkworm genome lacks an apparent ...
DOI:
10.1016/j.ibmb.2015.01.008

218. Cell Wall Diversity in Forage Maize: Genetic Complexity and Bioenergy Potential

Author:
Torres, Andres F.; Noordam-Boot, Cornelie M. M.; Dolstra, Oene; van der Weijde, Tim; Combes, Eliette; Dufour, Philippe; Vlaswinkel, Louis; Visser, Richard G. F.; Trindade, Luisa M.
Source:
BioEnergy research 2015 v.8 no.1 pp. 187-202
ISSN:
1939-1234
Subject:
chromosomes,   etc   ; alleles; bioenergy; biomass; biotransformation; cell wall components; cell walls; corn; cultivars; doubled haploids; forage; genetic variation; germplasm; glucose; hemicellulose; lignin; marker-assisted selection; quantitative trait loci; saccharification;     Show all 19 Subjects
Abstract:
... Genetic studies are ideal platforms for assessing the extent of genetic diversity, inferring the genetic architecture, and evaluating complex trait interrelations for cell wall compositional and bioconversion traits relevant to bioenergy applications. Through the characterization of a forage maize doubled haploid (DH) population, we indicate the substantial degree of highly heritable (h² > ~65 %) ...
DOI:
10.1007/s12155-014-9507-8

219. Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages

Author:
Elmore, M. Holly; McGary, Kriston L.; Wisecaver, Jennifer H.; Slot, Jason C.; Geiser, David M.; Sink, Stacy; O’Donnell, Kerry; Rokas, Antonis
Source:
Genome Biology and Evolution 2015 v.7 no.3 pp. 789-800
Subject:
chromosomes,   etc   ; Curvularia lunata; Fusarium oxysporum; carbonate dehydratase; cyanates; fungi; fungicides; gene flow; genes; hosts; humans; phylogeny; plant pathogens; strains; surveys; vascular wilt;     Show all 16 Subjects
Abstract:
... Fungi that have the enzymes cyanase and carbonic anhydrase show a limited capacity to detoxify cyanate, a fungicide employed by both plants and humans. Here, we describe a novel two-gene cluster that comprises duplicated cyanase and carbonic anhydrase copies, which we name the CCA gene cluster, trace its evolution across Ascomycetes, and examine the evolutionary dynamics of its spread among lineag ...
Handle:
10113/60627
DOI:
10.1093/gbe/evv025
PubMed:
25663439
PubMed Central:
PMC4438557

220. Detection of quantitative trait loci controlling UV-B resistance in soybean

Author:
Shim, Hyun-Chul; Ha, Bo-Keun; Yoo, Minho; Kang, Sung-Taeg
Source:
Euphytica 2015 v.202 no.1 pp. 109-118
ISSN:
0014-2336
Subject:
chromosome mapping; chromosomes,   etc   ; Glycine max; alleles; chlorosis; color; genetic markers; greenhouses; inbred lines; marker-assisted selection; microsatellite repeats; parents; petioles; phenotypic variation; plant breeding; plant damage; quantitative trait loci; soybeans; stress tolerance; ultraviolet radiation;     Show all 20 Subjects
Abstract:
... The depletion of stratospheric ozone has resulted in increased amounts of ultraviolet-B radiation (UV-B: 280–320 nm) reaching the Earth’s surface. Even a small increase in the incident UV-B radiation could cause significant biological effects in plants. In this study, we identified putative quantitative trait loci (QTL) associated with the resistance to enhanced UV-B radiation in soybean. A popula ...
DOI:
10.1007/s10681-014-1233-y

221. Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker

Author:
Ma, Wenwen; Wang, Xiaoyue; Mao, Yufeng; Wang, Zhiwen; Chen, Tao; Zhao, Xueming
Source:
Biotechnology letters 2015 v.37 no.3 pp. 609-617
ISSN:
0141-5492
Subject:
chromosomes,   etc   ; Corynebacterium glutamicum; genes; genetic engineering; genetic markers; models; polymerase chain reaction; screening;     Show all 8 Subjects
Abstract:
... Corynebacterium glutamicum is well-established for industrial and biotechnological applications. However, its genetic manipulation has generally lagged behind traditional genetic models. In this study, a counter-selectable marker gene upp was firstly confirmed to be more efficient than traditional sacB. Furthermore, a markerless gene replacement system was developed by combining upp with double-st ...
DOI:
10.1007/s10529-014-1718-8

222. Differential contributions of plant Dicer‐like proteins to antiviral defences against potato virus X in leaves and roots

Author:
Andika, Ida Bagus; Maruyama, Kazuyuki; Sun, Liying; Kondo, Hideki; Tamada, Tetsuo; Suzuki, Nobuhiro
Source:
The plant journal 2015 v.81 no.5 pp. 781-793
ISSN:
0960-7412
Subject:
chromosomes,   etc   ; Arabidopsis thaliana; Nicotiana benthamiana; Potato virus X; aerial parts; antiviral properties; antiviral proteins; genes; genetically modified organisms; green fluorescent protein; leaves; plant viruses; roots; shoots;     Show all 14 Subjects
Abstract:
... Members of the plant Dicer‐like (DCL) protein family are the critical components of the RNA‐silencing pathway that mediates innate antiviral defence. The distinct antiviral role of each individual DCL protein has been established with mostly based on observations of aerial parts of plants. Thus, although the roots are closely associated with the life cycle of many plant viruses, little is known ab ...
DOI:
10.1111/tpj.12770

223. Dissection of qTGW1.2 to three QTLs for grain weight and grain size in rice (Oryza sativa L.)

Author:
Wang, Lin-Lin; Chen, Yu-Yu; Guo, Liang; Zhang, Hong-Wei; Fan, Ye-Yang; Zhuang, Jie-Yun
Source:
Euphytica 2015 v.202 no.1 pp. 119-127
ISSN:
0014-2336
Subject:
chromosomes,   etc   ; quantitative trait loci; heterosis; isogenic lines; rice; phenotype; Oryza sativa; agronomic traits; additive gene effects; heading;     Show all 10 Subjects
Abstract:
... Dissection of qTGW1.2, a minor quantitative trait locus (QTL) for 1,000-grain weight located on the long arm of rice chromosome 1, was reported here. Six sets of near isogenic lines (NILs) were developed from six BC₂F₉plants of the indica rice cross Zhenshan 97³/Milyang 46, respectively, segregated in a sequential order covering the region for qTGW1.2. They were tested for 1,000-grain weight, grai ...
DOI:
10.1007/s10681-014-1237-7

224. Engineering Escherichia coli for autoinducible production of n-butanol

Author:
Wang, Qinglong; ding, Yi; Liu, Li; Shi, Jiping; Sun, Junsong; Xue, Yongchang
Source:
Electronic Journal of Biotechnology 2015 v.18 pp. 138-142
ISSN:
0717-3458
Subject:
chromosomes,   etc   ; DNA fragmentation; Escherichia coli; bioreactors; butanol; engineering; enzymes; excretion; feedstocks; fermentation; gene deletion; glucose; operon; plasmids; polymerase chain reaction;     Show all 15 Subjects
Abstract:
... Escherichia coli does not produce n-butanol naturally, but can be butanologenic when related enzymes were expressed using inducible elements on plasmids. In this study we attempted to confer E. coli strain capability of automatic excretion of the chemical by employing a native anaerobic promoter. Also, a novel DNA kit was designed for PCR preparation of linear DNA fragments to perform strain modif ...
DOI:
10.1016/j.ejbt.2015.01.003

225. Establishment and characterization of a new cell line of Chilo suppressalis Walker (Lepidoptera: Pyralididae)

Author:
Liu, Guangfu; Xu, Yipeng; Yu, Xiaoping
Source:
In vitro cellular & developmental biology 2015 v.51 no.3 pp. 218-221
ISSN:
1071-2690
Subject:
chromosomes,   etc   ; fetal bovine serum; Chilo suppressalis; Baculoviridae; DNA; Spodoptera exigua; polymerase chain reaction; fat body; larvae;     Show all 9 Subjects
Abstract:
... A new cell line, designated as ZJBIQ-Chsu-I, was initiated from the fat body of larval Chilo suppressalis (Walker) (Lepidoptera: Pyralididae) in TNM-FH insect medium containing 15% fetal bovine serum. The polygonal cells (65.6%) were predominant among various cell types, and the diameter range was from 12.63 to 22.50 μm. The cell line showed a typical lepidopteran chromosome pattern ranging from 1 ...
DOI:
10.1007/s11626-014-9831-5

226. Evolution of the Germline Actin Gene in Hypotrichous Ciliates: Multiple Nonscrambled IESs at Extremely Conserved Locations in Two Urostylids

Author:
Chen, Tianbing; Yi, Zhenzhen; Huang, Jie; Lin, Xiaofeng
Source:
The journal of eukaryotic microbiology 2015 v.62 no.2 pp. 188-195
ISSN:
1066-5234
Subject:
chromosomes,   etc   ; Sporadotrichida; actin; evolution; genes; germ cells;     Show all 6 Subjects
Abstract:
... In hypotrichous ciliates, macronuclear chromosomes are gene‐sized, and micronuclear genes contain short, noncoding internal eliminated segments (IESs) as well as macronuclear‐destined segments (MDSs). In the present study, we characterized the complete macronuclear gene and two to three types of micronuclear actin genes of two urostylid species, i.e. Pseudokeronopsis rubra and Uroleptopsis citrina ...
DOI:
10.1111/jeu.12158

227. F-actin participates in the process of the “partition-bundle division”

Author:
Chen, H.; Han, R.
Source:
Russian journal of plant physiology 2015 v.62 no.2 pp. 187-194
ISSN:
1021-4437
Subject:
chromosome aberrations; chromosomes,   etc   ; Triticum aestivum; actin; anaphase; interphase; metaphase; microfilaments; mitosis; prophase; seedlings; somatic cells; telophase; ultraviolet radiation; wheat;     Show all 15 Subjects
Abstract:
... A special kind of mitosis in wheat (Triticum aestivum L.) seedlings was observed after their exposure to the enhanced ultraviolet B (UV-B) radiation and named as “partition-bundle division”. To explore the abnormal mitosis, we focused on the variation of the actin filaments (F-actin) during the somatic cell division. The results showed that there are cyclic changes of F-actin during the cell cycle ...
DOI:
10.1134/S102144371502003X

228. Features of the B chromosome in Korean field mouse Apodemus peninsulae (Thomas, 1906) from Transbaikalia and the Far East identified by the FISH method

Author:
Rubtsov, N. B.; Kartavtseva, I. V.; Roslik, G. V.; Karamysheva, T. V.; Pavlenko, M. V.; Iwasa, M. A.; Koh, H. S.
Source:
Russian journal of genetics 2015 v.51 no.3 pp. 278-288
ISSN:
1022-7954
Subject:
chromosomes,   etc   ; Apodemus; DNA; DNA probes; fluorescence in situ hybridization; metaphase; mice; Japan; Siberia; South Korea;     Show all 10 Subjects
Abstract:
... Korean field mouse (Apodemus peninsulae) is widely distributed throughout northeastern Asia, including the Russian Far East, northern China, the Korean Peninsula, Sakhalin, and Hokkaido. This mouse species is characterized by a high frequency of animals with B chromosomes differing in their number, morphology, and DNA composition in different geographical regions. For the first time a comparative ...
DOI:
10.1134/S1022795415030114

229. Fine mapping of GRH3 conferring resistance to green rice leafhopper in rice (Oryza sativa L.)

Author:
Hur, Yeon-Jae; Cho, Jun Hyeon; Lee, Ji Yun; Sohn, Young-Bo; Park, Soo-Kwon; Lee, Bong-Choon; Cho, Jaemin; Kim, Sang Yeol; Song, You Chun; Park, Dong-Soo; Nam, Min-Hee; Lee, Jong Hee
Source:
Molecular breeding 2015 v.35 no.3 pp. 262
ISSN:
1380-3743
Subject:
chromosome mapping; chromosomes,   etc   ; Nephotettix nigropictus; Oryza sativa; cultivars; genetic markers; genetic resistance; genome; germplasm; insect pests; microsatellite repeats; parents; pest resistance; rice;     Show all 14 Subjects
Abstract:
... Green rice leafhopper (GRH) is a major sap-sucking insect pest that causes large annual yield losses in rice by transmitting the rice dwarf virus or spreading sooty mold fungi. Genetic resistance is the most effective approach to control GRH as well as the spread of diseases. We performed a genetic analysis to detect the GRH resistant region in rice cv. ‘Cheongnam’ using bulk segregant analysis of ...
DOI:
10.1007/s11032-015-0262-0

230. Fine mapping of the Asian soybean rust resistance gene Rpp2 from soybean PI 230970

Author:
Yu, Neil; Kim, Myungsik; King, Zachary R.; Harris, Donna K.; Buck, James W.; Li, Zenglu; Diers, Brian W.
Source:
Theoretical and applied genetics 2015 v.128 no.3 pp. 387-396
ISSN:
0040-5752
Subject:
chromosome mapping; chromosomes,   etc   ; Arabidopsis; Glycine max; Phakopsora pachyrhizi; disease resistance; genes; genetic markers; marker-assisted selection; microsatellite repeats; molecular cloning; progeny; single nucleotide polymorphism; soybean rust; soybeans;     Show all 15 Subjects
Abstract:
... KEY MESSAGE : Asian soybean rust (ASR) resistance gene Rpp2 has been fine mapped into a 188.1 kb interval on Glyma.Wm82.a2, which contains a series of plant resistance (R) genes. Asian soybean rust (ASR), caused by the fungus Phakopsora pachyrihizi Syd. & P. Syd., is a serious disease in major soybean [Glycine max (L.) Merr.] production countries worldwide and causes yield losses up to 75 %. Defin ...
DOI:
10.1007/s00122-014-2438-0

231. Fluorescence Assisted Selection of Transformants (FAST): Using flow cytometry to select fungal transformants

Author:
Vlaardingerbroek, Ido; Beerens, Bas; Shahi, Shermineh; Rep, Martijn
Source:
Fungal genetics and biology 2015 v.76 pp. 104-109
ISSN:
1087-1845
Subject:
chromosomes,   etc   ; Fusarium oxysporum; drug resistance; flow cytometry; fluorescence; fluorescent proteins; fungi; gene deletion; genes; genetic transformation; microbial genetics; mutants;     Show all 12 Subjects
Abstract:
... The availability of drug resistance markers for fungal transformation is often a limiting factor in both fungal genetics research and industrial applications. We describe a new technique using flow cytometry to select fungal transformants using well-known fluorescent proteins as markers for transformation. This new technique, Fluorescence-Assisted Selection of Transformants (FAST), was used for a ...
DOI:
10.1016/j.fgb.2015.02.003

232. Generation of a homozygous fertilization-defective gcs1 mutant by heat-inducible removal of a rescue gene

Author:
Nagahara, Shiori; Takeuchi, Hidenori; Higashiyama, Tetsuya
Source:
Plant reproduction 2015 v.28 no.1 pp. 33-46
ISSN:
2194-7953
Subject:
chromosomes,   etc   ; Arabidopsis thaliana; females; haploidy; heterozygosity; homozygosity; males; mutants; ova; ovules; pistil; plant reproduction; pollen tubes; spermatozoa; transgenes; zygote;     Show all 16 Subjects
Abstract:
... KEY MESSAGE : New gametic homozygous mutants. In angiosperms, a haploid male gamete (sperm cell) fuses with a haploid female gamete (egg cell) during fertilization to form a zygote carrying paternally and maternally derived chromosomes. Several fertilization-defective mutants in Arabidopsis thaliana, including a generative cell-specific 1 (gcs1)/hapless 2 mutant, the sperm cells of which are unabl ...
DOI:
10.1007/s00497-015-0256-4
PubMed:
25673573
PubMed Central:
PMC4333230

233. Genetic Architecture of Fusarium Head Blight Resistance in Four Winter Triticale Populations

Author:
Kalih, R.; Maurer, H. P.; Miedaner, T.
Source:
Phytopathology 2015 v.105 no.3 pp. 334-341
ISSN:
0031-949X
Subject:
chromosomes,   etc   ; Fusarium culmorum; Fusarium head blight; breeding; doubled haploids; genome; genotype; heading; phenotypic variation; quantitative trait loci; rye; triticale; wheat;     Show all 13 Subjects
Abstract:
... Fusarium head blight (FHB) is a devastating disease that causes significant reductions in yield and quality in wheat, rye, and triticale. In triticale, knowledge of the genetic architecture of FHB resistance is missing but essential due to modern breeding requirements. In our study, four doubled-haploid triticale populations (N = 120 to 200) were evaluated for resistance to FHB caused by artificia ...
DOI:
10.1094/PHYTO-04-14-0124-R

234. Genetic Characterization of Esterase Activity Variants Associated with an Esterase Gene Amplification in a Strain of Culex pipiens from California

Author:
Ferrari, James A.
Source:
Journal of the American Mosquito Control Association 2015 v.31 no.1 pp. 7-15
ISSN:
8756-971X
Subject:
chromosomes,   etc   ; Culex pipiens; acetates; alleles; aryldialkylphosphatase; electrophoresis; enzyme activity; gene amplification; genetic variance; genetic variation; insecticide resistance; variance; California;     Show all 13 Subjects
Abstract:
... In the Culex pipiens complex, a common mechanism of insecticide resistance is amplification of esterase genes leading to overproduction of detoxifying esterase enzymes. A number of electrophoretic esterase alleles have been identified, and in field populations individuals with the same esterase electromorph can exhibit a wide range of esterase enzyme activities. We isolated and characterized ester ...
DOI:
10.2987/14-6453R.1

235. Genetic characterization of the wheat association mapping initiative (WAMI) panel for dissection of complex traits in spring wheat

Author:
Lopes, M. S.; Dreisigacker, S.; Peña, R. J.; Sukumaran, S.; Reynolds, M. P.
Source:
Theoretical and applied genetics 2015 v.128 no.3 pp. 453-464
ISSN:
0040-5752
Subject:
chromosomes,   etc   ; Secale cereale; Triticum aestivum; alleles; genetic background; genetic markers; genotype-environment interaction; grain yield; heading; major genes; phenology; rye; single nucleotide polymorphism; spring wheat;     Show all 14 Subjects
Abstract:
... KEY MESSAGE : The wheat association mapping initiative is appropriate for gene discovery without the confounding effects of phenology and plant height. The wheat association mapping initiative (WAMI) population is a set of 287 diverse advanced wheat lines with a narrow range of variation for days to heading (DH) and plant height (PH). This study aimed to characterize the WAMI and showed that this ...
DOI:
10.1007/s00122-014-2444-2

236. Genetic mapping of QTL for maize weevil resistance in a RIL population of tropical maize

Author:
Castro-Álvarez, Fernando F.; William, Manilal; Bergvinson, David J.; García-Lara, Silverio
Source:
Theoretical and applied genetics 2015 v.128 no.3 pp. 411-419
ISSN:
0040-5752
Subject:
chromosome mapping; chromosomes,   etc   ; Sitophilus zeamais; Zea mays; adults; bioassays; corn; flour; genes; inbred lines; marker-assisted selection; pest resistance; phenotypic variation; progeny; quantitative trait loci; storage insects; stored grain; weight loss;     Show all 18 Subjects
Abstract:
... KEY MESSAGE : A tropical RIL maize population was subjected to phenotypic and genotypic analysis for maize weevil resistance during four seasons, and three main genomic areas were detected as main QTLs. The maize weevil (Sitophilus zeamais) (MW) is a common and important pest of stored maize (Zea mays) worldwide, especially in tropical areas. Quantitative trait loci (QTLs) associated with the MW h ...
DOI:
10.1007/s00122-014-2440-6

237. Genome-wide association study of production and stability traits in barley cultivated under future climate scenarios

Author:
Ingvordsen, C. H.; Backes, G.; Lyngkjær, M. F.; Peltonen-Sainio, P.; Jahoor, A.; Mikkelsen, T. N.; Jørgensen, R. B.
Source:
Molecular breeding 2015 v.35 no.3 pp. 283
ISSN:
1380-3743
Subject:
chromosomes,   etc   ; Hordeum vulgare; biomass; breeding; carbon dioxide; carbon dioxide enrichment; climate; climate change; cultivars; ears; genes; genetic markers; genome-wide association study; genotype; grain yield; harvest index; linear models; ozone; primary productivity; single nucleotide polymorphism; spring barley; temperature;     Show all 22 Subjects
Abstract:
... Future barley cultivars will have to produce under the constraints of higher temperature in combination with increased concentrations of atmospheric carbon dioxide and ozone as a consequence of climate change. A diverse set of 167 spring barley genotypes was cultivated under elevated levels of temperature (+5 °C) and [CO₂] (700 ppm) as single factors and in combination as well as under elevated [O ...
DOI:
10.1007/s11032-015-0283-8

238. Genome-wide identification and characterization of the aquaporin gene family in Sorghum bicolor (L.)

Author:
Reddy, Palakolanu Sudhakar; Rao, Tata Santosh Rama Bhadra; Sharma, Kiran K.; Vadez, Vincent
Source:
Plant gene 2015 v.1 pp. 18-28
ISSN:
2352-4073
Subject:
chromosomes,   etc   ; plant tissues; sequence analysis; plant development; plant stress; aquaporins; growth and development; Sorghum bicolor; environmental factors; gene expression; plant adaptation; abiotic stress; introns;     Show all 13 Subjects
Abstract:
... The aquaporin (AQP) gene family constitutes the most conserved class of gene families and plays a key role in water transport and conservation in plants. Although Sorghum genome sequencing has been completed, a comprehensive study of AQP genes in Sorghum bicolor is lacking. In the present study, we identified and characterized Sorghum AQP genes using a genome-wide scale, including factors such as ...
DOI:
10.1016/j.plgene.2014.12.002

239. Genomic Diversity of Saccharomyces cerevisiae Yeasts Associated with Alcoholic Fermentation of Bacanora Produced by Artisanal Methods

Author:
Álvarez-Ainza, M. L.; Zamora-Quiñonez, K. A.; Moreno-Ibarra, G. M.; Acedo-Félix, E.
Source:
Applied biochemistry and biotechnology 2015 v.175 no.5 pp. 2668-2676
ISSN:
0273-2289
Subject:
chromosomes,   etc   ; Agave angustifolia; Saccharomyces cerevisiae; alcoholic fermentation; bacteria; beverages; biotechnology; electrophoresis; genes; karyotyping; ribosomal DNA; traditional technology; yeasts;     Show all 13 Subjects
Abstract:
... Bacanora is a spirituous beverage elaborated with Agave angustifolia Haw in an artisanal process. Natural fermentation is mostly performed with native yeasts and bacteria. In this study, 228 strains of yeast like Saccharomyces were isolated from the natural alcoholic fermentation on the production of bacanora. Restriction analysis of the amplified region ITS1-5.8S-ITS2 of the ribosomal DNA genes ( ...
DOI:
10.1007/s12010-014-1469-y

240. Genotype-specific changes associated to early synthesis of autotetraploids in wild potato species

Author:
Aversano, Riccardo; Scarano, Maria-Teresa; Aronne, Giovanna; Caruso, Immacolata; D’Amelia, Vincenzo; De Micco, Veronica; Fasano, Carlo; Termolino, Pasquale; Carputo, Domenico
Source:
Euphytica 2015 v.202 no.2 pp. 307-316
ISSN:
0014-2336
Subject:
chromosome number; chromosomes,   etc   ; Solanum bulbocastanum; Solanum commersonii; diploidy; epigenetics; evolution; gene expression regulation; genes; genotype; interspecific hybridization; leaves; parents; polyploidy; potatoes;     Show all 15 Subjects
Abstract:
... Polyploidy is an important factor in plant evolution that may trigger drastic genome reorganization and phenotypic differentiation. In the last decade, extensive studies have been carried to understand the consequences of allopolyploidization, where the effects of ploidy change may be confounded by interspecific hybridization. By contrast, less is known on autopolyploidization, which only involves ...
DOI:
10.1007/s10681-014-1338-3

241. Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS

Author:
Shen, X. K.; Ma, L. X.; Zhong, S. F.; Liu, N.; Zhang, M.; Chen, W. Q.; Zhou, Y. L.; Li, H. J.; Chang, Z. J.; Li, X.; Bai, G. H.; Zhang, H. Y.; Tan, F. Q.; Ren, Z. L.; Luo, P. G.
Source:
Theoretical and applied genetics 2015 v.128 no.3 pp. 517-528
ISSN:
0040-5752
Subject:
chromosome mapping; chromosomes,   etc   ; Blumeria graminis f. sp. tritici; Brachypodium distachyon; Oryza sativa; Thinopyrum intermedium; Triticum aestivum; crop production; crossing; disease resistance; dominant genes; genetic distance; marker-assisted selection; microsatellite repeats; pedigree; powdery mildew; rice; wheat;     Show all 18 Subjects
Abstract:
... KEY MESSAGE: Powdery resistance putatively derived from Thinopyrum intermedium in the wheat line L962 is controlled by a single dominant gene designated PmL962 and mapped to chromosome arm 2BS. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting the production of wheat (Triticum aestivum). Powdery mildew resistance was putatively transferred from Th ...
DOI:
10.1007/s00122-014-2449-x

242. Identification and mapping of stable QTL for protein content in soybean seeds

Author:
Wang, J.; Chen, P.; Wang, D.; Shannon, G.; Zeng, A.; Orazaly, M.; Wu, C.
Source:
Molecular breeding 2015 v.35 no.3 pp. 285
ISSN:
1380-3743
Subject:
chromosome mapping; chromosomes,   etc   ; Glycine max; crossing; genetic markers; inbred lines; marker-assisted selection; microsatellite repeats; oils; protein content; quantitative trait loci; seeds; single nucleotide polymorphism; soybeans;     Show all 14 Subjects
Abstract:
... Protein and oil are two primary traits in soybean, which affect the quality of soyfood, feed, and oil products. The objectives of this research were to identify and validate quantitative trait loci (QTL) for seed protein. Crosses were made between two high-protein and two low-protein lines, R05-1415 × R05-638 designated as Population 1, and V97-1346 × R05-4256 as Population 2. A total of 242 and 2 ...
DOI:
10.1007/s11032-015-0285-6

243. Identification and phylogenetic analysis of late embryogenesis abundant proteins family in tomato (Solanum lycopersicum)

Author:
Cao, Jun; Li, Xiang
Source:
Planta 2015 v.241 no.3 pp. 757-772
ISSN:
0032-0935
Subject:
chromosomes,   etc   ; Solanum lycopersicum; abiotic stress; embryogenesis; genes; genomics; intragenic recombination; microarray technology; phylogeny; promoter regions; proteins; quantitative polymerase chain reaction; tissues; tomatoes;     Show all 14 Subjects
Abstract:
... MAIN CONCLUSION : This study provided a comparative genomic analysis of the LEA gene family, and these may provide valuable information for their functional investigations in the future. Late embryogenesis abundant (LEA) proteins are a group of proteins that accumulate in response to cellular dehydration in many organisms. Here, we identified 27 LEA genes in tomato. A strong correlation between ph ...
DOI:
10.1007/s00425-014-2215-y

244. Identification of potentially invasive species of black flies [Diptera: Simuliidae] from Armenia based on an analysis of variability in the mtDNA barcode of the cox1 gene and chromosomal polymorphism

Author:
Andrianov, B. V.; Goryacheva, I. I.; Vlasov, S. V.; Gorelova, T. V.; Harutyunova, M. V.; Harutyunova, K. V.; Mayilyan, K. R.; Zakharov, I. A.
Source:
Russian journal of genetics 2015 v.51 no.3 pp. 289-299
ISSN:
1022-7954
Subject:
chromosome morphology; chromosomes,   etc   ; Simulium; barcoding; fauna; genes; invasive species; mitochondrial DNA; morphometry; Armenia;     Show all 10 Subjects
Abstract:
... Black flies (Diptera, Simuliidae) are well known for their medical, environmental, and veterinary importance. The simuliid fauna of Armenia includes 53 species. A number of dominant species are of ecological importance. Complex analysis, which involved morphometric, cytogenetic, and molecular genetic approaches, was conducted to characterize the species status of black flies inhabiting the territo ...
DOI:
10.1134/S1022795415020039

245. Identification of QTL for seed coat colour and oil content in Brassica napus by association mapping using SSR markers

Author:
Qu, Cunmin; Hasan, Maen; Lu, Kun; Liu, Liezhao; Zhang, Kai; Fu, Fuyou; Wang, Min; Liu, Shuiyan; Bu, Haidong; Wang, Rui; Xu, Xinfu; Chen, Li; Li, Jiana
Source:
Canadian journal of plant science 2015 v.95 no.2 pp. 387-395
ISSN:
1918-1833
Subject:
chromosome mapping; chromosomes,   etc   ; Brassica napus; color; genotype; germplasm; linkage disequilibrium; linkage groups; lipid content; marker-assisted selection; microsatellite repeats; population structure; quantitative trait loci; seed coat; seed oils;     Show all 15 Subjects
Abstract:
... Qu, C., Hasan, M., Lu, K., Liu, L., Zhang, K., Fu, F., Wang, M., Liu, S., Bu, H., Wang, R., Xu, X., Chen, L. and Li, J. 2015. Identification of QTL for seed coat colour and oil content in Brassica napus by association mapping using SSR markers. Can. J. Plant Sci. 95: 387–395. Association mapping identifies quantitative trait loci (QTL) based on the strength of linkage disequilibrium (LD) between m ...
DOI:
10.4141/cjps2013-411

246. Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

Author:
Yasui, G. S.; Senhorini, J. A.; Shimoda, E.; Pereira-Santos, M.; Nakaghi, L. S. O.; Fujimoto, T.; Arias-Rodriguez, L.; Silva, L. A.
Source:
Animal 2015 v.9 no.3 pp. 464-470
ISSN:
1751-732X
Subject:
chromosomes,   etc   ; Astyanax altiparanae; antibiotics; biotechnology; carp; females; in vitro fertilization; males; oocytes; oxygen; pituitary gland; semen; sodium chloride; spawning; sperm motility; spermatozoa; temperature;     Show all 17 Subjects
Abstract:
... In fish, in vitro fertilization is an important reproductive tool used as first step for application of others biotechniques as chromosome and embryo manipulation. In this study, we aimed to optimize gamete quality and their short-term storage from the yellowtail tetra Astyanax altiparanae, for future application in laboratory studies. Working with sperm, we evaluated the effects of spawning induc ...
DOI:
10.1017/S1751731114002511
PubMed:
25391393

247. Induction of somatic embryogenesis by anther-derived callus culture and plantlet ploidy determination in poplar (Populus × beijingensis )

Author:
Huang, Zhen; Xu, Congping; Li, Yun; Wang, Peiqi; Li, Yuan; Kang, Xiangyang
Source:
Plant cell, tissue, and organ culture 2015 v.120 no.3 pp. 949-959
ISSN:
0167-6857
Subject:
chromosomes,   etc   ; Populus; benzyladenine; callus; callus culture; centrifugation; diploidy; flow cytometry; in vitro regeneration; naphthaleneacetic acid; organ culture; plantlets; sieving; somatic embryogenesis; tetraploidy;     Show all 15 Subjects
Abstract:
... In vitro regeneration through somatic embryogenesis using anther-derived callus from poplar (P. × beijingensis) has been reported. Calli induced from anther connective tissue were used to create suspension cell. Embryogenic cells were enriched by sieving and centrifugation, then embryogenic cells were shaking cultured in liquid medium lacking NH₄⁺to incubate round callus (RC). Thick slices of RC i ...
DOI:
10.1007/s11240-014-0649-3

248. Insights from 20 years of bacterial genome sequencing

Author:
Land, Miriam; Hauser, Loren; Jun, Se-Ran; Nookaew, Intawat; Leuze, Michael R.; Ahn, Tae-Hyuk; Karpinets, Tatiana; Lund, Ole; Kora, Guruprased; Wassenaar, Trudy; Poudel, Suresh; Ussery, David W.
Source:
Functional & integrative genomics 2015 v.15 no.2 pp. 141-161
ISSN:
1438-793X
Subject:
chromosomes,   etc   ; Escherichia coli; bacteria; bacterial communities; epidemiology; genes; high-throughput nucleotide sequencing; infectious diseases; metagenomics; methylation; models; nucleotide sequences; pathogens; patients; viruses;     Show all 15 Subjects
Abstract:
... Since the first two complete bacterial genome sequences were published in 1995, the science of bacteria has dramatically changed. Using third-generation DNA sequencing, it is possible to completely sequence a bacterial genome in a few hours and identify some types of methylation sites along the genome as well. Sequencing of bacterial genome sequences is now a standard procedure, and the informatio ...
DOI:
10.1007/s10142-015-0433-4
PubMed:
25722247
PubMed Central:
PMC4361730

249. In silico identification and expression analysis of MscS like gene family in rice

Author:
Saddhe, Ankush Ashok; Kumar, Kundan
Source:
Plant gene 2015 v.1 pp. 8-17
ISSN:
2352-4073
Subject:
chromosomes,   etc   ; abiotic stress; blood pressure; gene expression; genes; gravity; hearing; membrane proteins; mitochondria; osmotic pressure; pain; phylogeny; plasma membrane; plastids; prediction; proprioception; rice; sounds;     Show all 18 Subjects
Abstract:
... Mechanosensitive channels are membrane proteins that open and shut in response to mechanical forces produced by osmotic pressure, sound, touch and gravity. These channels are involved in multiple physiological functions including hypoosmotic pressure, pain, hearing, blood pressure and cell volume regulation. In plants, these channels play a major role in proprioception, gravity sensing and mainten ...
DOI:
10.1016/j.plgene.2014.12.001

250. Interaction between Nef and INI1/SMARCB1 augments replicability of HIV-1 in resting human peripheral blood mononuclear cells

Author:
Pyeon, Dohun; Park, In-Woo
Source:
Archives of virology 2015 v.160 no.3 pp. 727-737
ISSN:
0304-8608
Subject:
chromosomes,   etc   ; DNA; HIV infections; Human immunodeficiency virus 1; T-lymphocytes; humans; nucleotides; proviruses; two hybrid system techniques; virus replication;     Show all 10 Subjects
Abstract:
... A central feature of HIV-1 infection is the inability of entering virus to integrate into chromosomes of resting T lymphocytes unless they are mitogenically activated. In contrast, SIVpbj1.9 replicates in initially resting T lymphocytes by activating infected cells. Previous reports have shown that a difference in Nef-mediated T cell activation between HIV-1 and SIVpbj1.9 plays a critical role in ...
DOI:
10.1007/s00705-014-2315-9
PubMed:
25559666
PubMed Central:
PMC4339447