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- Isupov, Michail N., et al. Show all 4 Authors
- The FEBS journal 2015 v.282 no.15 pp. 2846-2857
- Escherichia coli; acetates; active sites; alcohols; amino acids; butyrates; catalytic activity; crystal structure; esterases; genetic databases; ligands; malates; mutants; propionic acid; thermophilic bacteria
- ... Thermogutta terrifontis esterase (TtEst), a carboxyl esterase identified in the novel thermophilic bacterium T. terrifontis from the phylum Planctomycetes, has been cloned and over‐expressed in Escherichia coli. The enzyme has been characterized biochemically and shown to have activity towards small p‐nitrophenyl (pNP) carboxylic esters, with optimal activity for pNP‐propionate. The enzyme retaine ...