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- Prazeres, Duarte M. F., et al. Show all 7 Authors
- Journal of chemical technology and biotechnology 2015 v.90 no.2 pp. 255-261
- Escherichia coli; acetates; biomass; bioprocessing; carbon; culture media; fiber optics; genes; glucose; glycerol; least squares; models; monitoring; near-infrared spectroscopy; pharmaceutical industry; plasmids; prediction
- ... BACKGROUND: While the pharmaceutical industry keeps an eye on plasmid DNA production for new generation gene therapies, real‐time monitoring techniques for plasmid bioproduction are as yet unavailable. This work shows the possibility of in situ monitoring of plasmid production in Escherichia coli cultures using a near infrared (NIR) fiber optic probe. RESULTS: Partial least squares (PLS) regressio ...