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1. Suppressive effect of SATB1 on hepatic stellate cell activation and liver fibrosis in rats

Author:
He, Jiayi; Gong, Jin; Ding, Qiang; Tan, Qinghai; Han, Ping; Liu, Jingmei; Zhou, Zhenzhen; Tu, Wei; Xia, Yujia; Yan, Wei; Tian, Dean
Source:
FEBS letters 2015 v.589 pp. 1359-1368
ISSN:
0014-5793
Subject:
rats,   etc   ; collagen; fibrosis; liver; liver cirrhosis;     Show all 5 Subjects
Abstract:
... Liver fibrosis is a worldwide clinical issue. Activation of hepatic stellate cells (HSCs) is the central event during liver fibrosis. We investigated the role of SATB1 in HSC activation and liver fibrogenesis. The results show that SATB1 expression is reduced during HSC activation. Additionally, SATB1 inhibits HSC activation, proliferation, migration, and collagen synthesis. Furthermore, CTGF, a p ...
DOI:
10.1016/j.febslet.2015.04.010

2. Metabolic transformation as a diagnostic tool for the selection of candidate promutagens in effect-directed analysis

Author:
Hug, Christine; Krauss, Martin; Nüsser, Leonie; Hollert, Henner; Brack, Werner
Source:
Environmental pollution 2015 v.196 pp. 114-124
ISSN:
0269-7491
Subject:
rats,   etc   ; liver; metabolism; metabolites; mutagenicity; wastewater treatment;     Show all 6 Subjects
Abstract:
... To implement metabolic activation by S9 rat liver homogenate in the selection of candidate promutagens in effect-directed analysis, we critically assessed the capability of LC-HRMS measurements to detect depletion and formation of metabolites by S9 exposure. The exposure of a reference mixture to S9 led to a depletion by >70% for most compounds. Other processes than metabolism were excluded as sig ...
DOI:
10.1016/j.envpol.2014.10.003

3. Chronic exposure to hexachlorobenzene results in down-regulation of connexin43 in the breast

Author:
Delisle, Ariane; Ferraris, Emanuelle; Plante, Isabelle
Source:
Environmental Research 2015 v.143 pp. 229-240
ISSN:
0013-9351
Subject:
rats,   etc   ; chronic exposure; connexins; females; hepatocytes; hexachlorobenzene; liver; neoplasms; tissues;     Show all 9 Subjects
Abstract:
... Decreased expression of connexins has been associated with cancer, but the underlying mechanisms are poorly understood. We have previously shown that a 5 day exposure to hexachlorobenzene (HCB) resulted in decreased connexins expression in hepatocytes 45 days later, and that this down-regulation was linked to activation of Akt through the ILK pathway. Because HCB promotes cancer in both the liver ...
DOI:
10.1016/j.envres.2015.10.020

4. 3D spherical microtissues and microfluidic technology for multi-tissue experiments and analysis

Author:
Kim, Jin-Young; Fluri, David A.; Marchan, Rosemarie; Boonen, Kurt; Mohanty, Soumyaranjan; Singh, Prateek; Hammad, Seddik; Landuyt, Bart; Hengstler, Jan G.; Kelm, Jens M.; Hierlemann, Andreas; Frey, Olivier
Source:
Journal of biotechnology 2015 v.205 pp. 24-35
ISSN:
0168-1656
Subject:
rats,   etc   ; coculture; colorectal neoplasms; cyclophosphamide; liver; models; pumps;     Show all 7 Subjects
Abstract:
... Rational development of more physiologic in vitro models includes the design of robust and flexible 3D-microtissue-based multi-tissue devices, which allow for tissue–tissue interactions. The developed device consists of multiple microchambers interconnected by microchannels. Pre-formed spherical microtissues are loaded into the microchambers and cultured under continuous perfusion. Gravity-driven ...
DOI:
10.1016/j.jbiotec.2015.01.003

5. Chronological protein synthesis in regenerating rat liver

Author:
He, Jinjun; Hao, Shuai; Zhang, Hao; Guo, Fuzheng; Huang, Lingyun; Xiao, Xueyuan; He, Dacheng
Source:
Electrophoresis 2015 v.36 no.14 pp. 1622-1632
ISSN:
0173-0835
Subject:
rats,   etc   ; electrophoresis; liver; liver regeneration; protein synthesis; proteins; proteomics;     Show all 7 Subjects
Abstract:
... Liver regeneration has been studied for decades; however, its regulation remains unclear. In this study, we report a dynamic tracing of protein synthesis in rat regenerating liver with a new proteomic technique, ³⁵S in vivo labeling analysis for dynamic proteomics (SiLAD). Conventional proteomic techniques typically measure protein alteration in accumulated amounts. The SiLAD technique specificall ...
DOI:
10.1002/elps.201500019

6. Enhancement of cysteine catabolism into taurine impacts glutathione homeostasis in rats challenged with ethanol

Author:
Ahn, Chul Won; Kwon, Do Young; Jun, Doo Sung; Lee, Yoo Min; Kim, Young Chul
Source:
Amino acids 2015 v.47 no.6 pp. 1273-1277
ISSN:
0939-4451
Subject:
rats,   etc   ; cysteine; ethanol; glutathione; homeostasis; liver; metabolism; taurine;     Show all 8 Subjects
Abstract:
... We determined the alterations in metabolic conversion of cysteine into glutathione and taurine in liver of rats treated with ethanol acutely. Ethanol treatment reduced cysteine as well as glutathione levels in liver for 24 h. However, cysteine dioxygenase was up-regulated rapidly, and hypotaurine/taurine levels were significantly higher than those found in the saline-treated rats. It is therefore ...
DOI:
10.1007/s00726-015-1969-2

7. Comparative analysis of subpopulations of mesenchymal stromal cells of the bone marrow and fetal liver differing in sensitivity to 5-fluorouracil

Author:
Payushina, O. V.; Butorina, N. N.; Sheveleva, O. N.; Domaratskaya, E. I.
Source:
Biology bulletin of the Russian Academy of Sciences 2015 v.42 no.3 pp. 206-212
ISSN:
1062-3590
Subject:
rats,   etc   ; bone marrow; bone marrow cells; liver; stromal cells;     Show all 5 Subjects
Abstract:
... It is shown that the effect of 5-fluorouracil (5-FU) on mesenchymal stromal cells (MSCs) of the bone marrow and fetal liver of the rat enriches the population with cells with a reduced proliferative potential. It is shown that, in the course of passaging, the 5-FU-resistant MSCs of the bone marrow and fetal liver lose their ability to differentiate in the osteogenic direction more and less rapidly ...
DOI:
10.1134/S1062359015030097

8. Characteristics of regulation of the activity of glucokinase from rat liver

Author:
Khu, L. Ya.; Goncharova, N. Yu.; Rubtsov, A. M.
Source:
Moscow University biological sciences bulletin 2015 v.70 no.1 pp. 12-16
ISSN:
0096-3925
Subject:
rats,   etc   ; enzyme activity; glucokinase; glucose; hexokinase; isolation techniques; liver;     Show all 7 Subjects
Abstract:
... A simple method for isolation of glucokinase from a soluble fraction of rat liver was proposed, making it possible to obtain the enzyme preparation characterized by high specific activity, native cooperative characteristics of the enzyme, and actually complete absence of other molecular forms of hexokinase. Dependence of the enzyme activity on the glucose concentration shows a pronounced sigmoidal ...
DOI:
10.3103/S0096392515010058

9. Diamide accelerates opening of the Tl+-induced permeability transition pore in Ca2+-loaded rat liver mitochondria

Author:
Korotkov, Sergey M.; Konovalova, Svetlana A.; Brailovskaya, Irina V.
Source:
Biochemical and biophysical research communications 2015 v.468 pp. 360-364
ISSN:
0006-291X
Subject:
rats,   etc   ; adenine; adenosine diphosphate; calcium; cyclosporine; glutathione; liver; membrane potential; mitochondria; oxidants; permeability;     Show all 11 Subjects
Abstract:
... Opening of the mitochondrial permeability transition pore (MPTP) in the inner membrane is due to matrix Ca2+ overload and matrix glutathione loss. Fixing the ‘m’ conformation of the adenine nucleotide translocase (ANT) by ADP or N-ethylmaleimide (NEM) inhibits opening of the MPTP. Oxidants (diamide or tert-butylhydroperoxide (tBHP)) fix the ANT in ‘c’ conformation, and the ability of ADP to inhibi ...
DOI:
10.1016/j.bbrc.2015.10.091

10. Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats

Author:
Guo, Xiaochun; Chen, Liang; Chen, Jun; Xie, Ping; Li, Shangchun; He, Jun; Li, Wei; Fan, Huihui; Yu, Dezhao; Zeng, Cheng
Source:
Environmental science and pollution research international 2015 v.22 no.23 pp. 19273-19284
ISSN:
0944-1344
Subject:
rats,   etc   ; Cyanobacteria; antioxidants; glutathione; hepatotoxicity; lethal dose 50; liver; metabolism; metabolites; microcystin-LR;     Show all 10 Subjects
Abstract:
... Glutathione (GSH) plays crucial roles in antioxidant defense and detoxification metabolism of microcystin-LR (MC-LR). However, the detoxification process of MC-LR in mammals remains largely unknown. This paper, for the first time, quantitatively analyzes MC-LR and its GSH pathway metabolites (MC-LR-GSH and MC-LR-Cys) in the liver of Sprague–Dawley (SD) rat after MC-LR exposure. Rats received intra ...
DOI:
10.1007/s11356-015-5531-2

11. Healthy aspect of low‐cholesterol ghee on modulation of lipid profile of rats

Author:
Meghwal, Karuna; Sharma, Vivek; Lal, Darshan; Arora, Sumit; Kapila, Suman
Source:
International journal of dairy technology 2015 v.68 no.4 pp. 550-556
ISSN:
1364-727X
Subject:
rats,   etc   ; arteries; ghee; high density lipoprotein cholesterol; liver; low density lipoprotein cholesterol; triacylglycerols;     Show all 7 Subjects
Abstract:
... Low‐cholesterol ghee (LCG) feeding did not show any significant increase in total plasma cholesterol level from day 0 (56.8 ± 6.8 mg/dL) to day 90 (59.5 ± 0.8 mg/dL). Plasma HDL cholesterol (HDL) levels increased by 59.9%, whereas LDL cholesterol (LDL) showed a reduction of 74.8%. Total plasma triacylglycerol (mg/dL) did not show any significant change within or between groups. Relative contributi ...
DOI:
10.1111/1471-0307.12206

12. Enhanced SDC-assisted digestion coupled with lipid chromatography-tandem mass spectrometry for shotgun analysis of membrane proteome

Author:
Lin, Yong; Wang, Kunbo; Liu, Zhonghua; Lin, Haiyan; Yu, Lijun
Source:
Journal of Chromatography B 2015 v.1002 pp. 144-151
ISSN:
1570-0232
Subject:
rats,   etc   ; chromatography; deoxycholic acid; hydrophobicity; lipids; liver; membrane proteins; peptides; proteome; proteomics; solubilization; tandem mass spectrometry;     Show all 12 Subjects
Abstract:
... Despite the biological importance of membrane proteins, their analysis has lagged behind that of soluble proteins and still presents a great challenge mainly because of their highly hydrophobic nature and low abundance. Sodium deoxycholate (SDC)-assisted digestion strategy has been introduced in our previous papers, which cleverly circumvents many of the challenges in shotgun membrane proteomics. ...
DOI:
10.1016/j.jchromb.2015.08.019

13. Expression of Iodotyrosine Deiodinase in Thyroid and Other Organs in Iodine-Deficient and Iodine-Excess Rats

Author:
Sun, Xin; Zhang, Xiaowen; Jiang, Yaqiu; Bao, Suqing; Shan, Zhongyan; Teng, Weiping
Source:
Biological trace element research 2015 v.167 no.2 pp. 272-279
ISSN:
0163-4984
Subject:
rats,   etc   ; dehalogenation; females; homeostasis; humans; iodide peroxidase; iodine; iodine deficiency; kidneys; liver;     Show all 10 Subjects
Abstract:
... In mammals, iodothyronine deiodinase and iodotyrosine deiodinase (IYD) are known to catalyze the reductive dehalogenation. IYD is a critical enzyme in maintaining iodine homeostasis. Advances in the study of iodothyronine deiodinase have been published steadily; research on IYD has been slow on its function and regulation. We studied the expression of IYD in thyroid, liver, and kidney in condition ...
DOI:
10.1007/s12011-015-0328-1

14. Static magnetic field exposure–induced oxidative response and caspase-independent apoptosis in rat liver: effect of selenium and vitamin E supplementations

Author:
Ghodbane, Soumaya; Ammari, Mohamed; Lahbib, Aida; Sakly, Mohsen; Abdelmelek, Hafedh
Source:
Environmental science and pollution research international 2015 v.22 no.20 pp. 16060-16066
ISSN:
0944-1344
Subject:
rats,   etc   ; apoptosis; brain; catalase; liver; magnetic fields; malondialdehyde; oxidative stress; protective effect; selenium; tissues; vitamin E;     Show all 12 Subjects
Abstract:
... In the present study, we investigated the implication of oxidative stress and apoptosis under static magnetic field (SMF) in the brain and liver. Moreover, we estimated the protective role of selenium and vitamin E in rat tissues against disorders induced by SMF. Exposure of rats to SMF (128 mT, 1 h/day during five consecutive days) increased the activity of catalase (CAT) (+24 %) in the liver but ...
DOI:
10.1007/s11356-015-4802-2

15. Thrombopoietin Signaling Pathway Regulates Hepatocyte Activation in Rat Liver Regeneration

Author:
Chang, Cuifang; Yang, Jing; Li, Xiaofang; Zhao, Weiming; Li, Yu; Guo, Pengjuan; Wang, Gaiping; Xu, Cunshuan
Source:
Biochemical genetics 2015 v.53 no.9-10 pp. 244-259
ISSN:
0006-2928
Subject:
rats,   etc   ; gene expression; gene expression regulation; genes; hepatocytes; liver; liver regeneration; signal transduction; thrombopoietin;     Show all 9 Subjects
Abstract:
... Thrombopoietin (THPO) signaling pathway regulates cell activation and many other physiological activities. To study its role in liver regeneration (LR), hepatocytes were isolated from rat regenerating livers and gene expression profile was detected using the Rat Genome 230 2.0 Array. Spectral function (E ₜ) and information correlation coefficient (ICC) were used to analyze gene synergy based on ge ...
DOI:
10.1007/s10528-015-9685-x

16. Toxicity of zinc oxide nanoparticles on adult male Wistar rats

Author:
Abbasalipourkabir, Roghayeh; Moradi, Hemen; Zarei, Sadegh; Asadi, Soheila; Salehzadeh, Aref; Ghafourikhosroshahi, Abolfazl; Mortazavi, Motahareh; Ziamajidi, Nasrin
Source:
Food and chemical toxicology 2015 v.84 pp. 154-160
ISSN:
0278-6915
Subject:
rats,   etc   ; adults; blood serum; enzymes; histopathology; liver; males; nanoparticles; oxidative stress; spermatozoa; toxicity; toxicology; zinc oxide;     Show all 13 Subjects
Abstract:
... The purpose of this study was to investigate the effects of zinc oxide nanoparticles (nZnO) on adult male Wistar rats.Thirty male Wistar rats divided into five groups of six animals each were used for this study. For ten days, Groups one to four continuously received 50, 100, 150 and 200 mg/kg nZnO, respectively. Group five served as the control group. At the end of the study, the rats were sacrif ...
DOI:
10.1016/j.fct.2015.08.019

17. Circadian variation in hepatic toxicity of the immunosuppressive agent “Mycophenolate Mofetil” in rats

Author:
Dridi, Ichrak; Ben-Cherif, Wafa; Aouam, Karim; Haouas, Zohra; Ben-Attia, Mossadok; Reinberg, Alain; Boughattas, Naceur A.
Source:
Biological rhythm research 2015 v.46 no.5 pp. 739-751
ISSN:
1744-4179
Subject:
rats,   etc   ; alkaline phosphatase; circadian rhythm; diurnal activity; drugs; hepatotoxicity; histopathology; liver; patients;     Show all 9 Subjects
Abstract:
... Immunosuppressive drugs may have varying toxicity or efficacy depending on the administration time. This study investigates whether the liver toxicity of the mycophenolate mofetil (MMF) varies according to the circadian dosing-time in rats. 300 mg/kg of MMF was injected by intraperitonal route to different groups of animals at four different circadian stages (1, 7, 13, and 19 h after light onset, ...
DOI:
10.1080/09291016.2015.1052648

18. Metabolites characterization of timosaponin AIII in vivo and in vitro by using liquid chromatography-mass spectrometry

Author:
Sun, Yu; Liu, Liyin; Peng, Ying; Liu, Bingjie; Lin, Dongju; Li, Lingzhi; Song, Shaojiang
Source:
Journal of Chromatography B 2015 v.997 pp. 236-243
ISSN:
1570-0232
Subject:
rats,   etc   ; bile; drugs; feces; liquid chromatography; liver; mass spectrometry; medicinal properties; metabolism; metabolites; saponins; spectrometers; urine;     Show all 13 Subjects
Abstract:
... Timosaponin AIII, a major saponin found in Anemarrhena asphodeloides Bge., exhibits a wide spectrum of bioactivities. It is believed that it may be further developed into a promising new drug. To better understand the pharmacological activities of the component, the investigation of its in vivo and in vitro metabolism was necessary. In this study, the metabolic profile of timosaponin AIII was inve ...
DOI:
10.1016/j.jchromb.2015.06.015

19. Adaptations of Arginine's Intestinal-Renal Axis in Cachectic Tumor-Bearing Rats

Author:
Buijs, Nikki; Vermeulen, Mechteld A. R.; Weeda, Viola B.; Bading, James R.; Houdijk, Alexander P. J.; van Leeuwen, Paul A. M.
Source:
Nutrition and cancer 2015 v.67 no.5 pp. 713-720
ISSN:
1532-7914
Subject:
rats,   etc   ; arginine; cachexia; citrulline; diet; glutamine; immune system; intestines; kidneys; liver; metabolism; neoplasms; skeletal muscle;     Show all 13 Subjects
Abstract:
... Malignancies induce disposal of arginine, an important substrate for the immune system. To sustain immune function, the tumor-bearing host accelerates arginine's intestinal-renal axis by glutamine mobilization from skeletal muscle and this may promote cachexia. Glutamine supplementation stimulates argi-nine production in healthy subjects. Arginine's intestinal-renal axis and the effect of glutamin ...
DOI:
10.1080/01635581.2015.1029638

20. 2,2,2-Trichloroethanol lengthens the circadian period of Bmal1-driven circadian bioluminescence rhythms in U2OS cells

Author:
Gao, Jie; Yang, Caixia; Ding, Hui; Zhang, Yanli; Xue, Jinhua; Cai, Yanning
Source:
Biochemical and biophysical research communications 2015 v.462 pp. 239-244
ISSN:
0006-291X
Subject:
rats,   etc   ; anesthesia; bioluminescence; blood serum; chloral hydrate; circadian rhythm; humans; liver; metabolites; mice; trichloroethylene;     Show all 11 Subjects
Abstract:
... 2,2,2-Trichloroethanol (TCOH) is responsible for the pharmacological actions of chloral hydrate (CH), and is a major metabolite of trichloroethylene. Human exposure to TCOH is known to be increasing. Recently, it was reported that TCOH causes a significant phase delay of Per2 expression in mouse liver when injected daily over the course of several days. However, it is not clear whether TCOH direct ...
DOI:
10.1016/j.bbrc.2015.04.123