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1. Laccase initiated CC couplings: Various techniques for reaction monitoring

Author:
Engelmann, Claudia; Illner, Sabine; Kragl, Udo
Source:
Process Biochemistry 2015 v.50 pp. 1591-1599
ISSN:
1359-5113
Subject:
Myceliophthora thermophila,   etc   ; Fourier transform infrared spectroscopy; antibiotics; drugs; fungi; laccase; monitoring; oxidants; oxidation; oxygen;     Show all 10 Subjects
Abstract:
... In this study the fungal laccase from Myceliophthora thermophila (Novozym® 51003) was investigated for oxidative CC couplings of phenolic compounds. This enzyme requires only molecular oxygen as oxidant, which represents a great advantage for oxidative coupling reactions. However, such couplings are typically considered as highly unselective, due to the radical reaction mechanism of laccases. Base ...
DOI:
10.1016/j.procbio.2015.05.011

2. Synthesis and enzymatic hydrolysis of a diaryl benzyl ester model of a lignin-carbohydrate complex (LCC)

Author:
Nylander, Filip; Sunner, Hampus; Olsson, Lisbeth; Christakopoulos, Paul; Westman, Gunnar
Source:
Holzforschung 2015 v.70 no.5 pp. 385-391
ISSN:
1437-434X
Subject:
Myceliophthora thermophila,   etc   ; aqueous solutions; biopolymers; enzymatic hydrolysis; esterases; glucuronic acid; hydrolysis; lignin; lignocellulose; models; pH; xylan;     Show all 12 Subjects
Abstract:
... Specific degradation of the bonds between lignin and carbohydrates is an important step towards separating individual lignocellulosic biopolymers for sustainable production of materials and chemicals. One of the most established covalent lignin-carbohydrate (LC) interactions is the ester bond between the α- or γ-hydroxyl group of a lignin phenylpropane unit and a glucuronic acid side chain of xyla ...
DOI:
10.1515/hf-2014-0347

3. Ethanol Production from Enzymatically Treated Dried Food Waste Using Enzymes Produced On-Site

Author:
Matsakas, Leonidas; Christakopoulos, Paul
Source:
Sustainability 2015 v.7 no.2 pp. 1446-1458
ISSN:
2071-1050
Subject:
Myceliophthora thermophila,   etc   ; biofuels; cellulose; enzymes; ethanol; ethanol production; fermentation; food production; food waste; fungi; hydrolysis; saccharification;     Show all 12 Subjects
Abstract:
... The environmental crisis and the need to find renewable fuel alternatives have made production of biofuels an important priority. At the same time, the increasing production of food waste is an important environmental issue. For this reason, production of ethanol from food waste is an interesting approach. Volumes of food waste are reduced and ethanol production does not compete with food producti ...
DOI:
10.3390/su7021446

4. Glucoamylases from Penicillium verruculosum and Myceliophthora thermophila: Analysis of differences in activity against polymeric substrates based on 3D model structures of the intact enzymes

Author:
Volkov, Pavel V.; Rozhkova, Aleksandra M.; Gusakov, Alexander V.; Zorov, Ivan N.; Sinitsyn, Arkady P.
Source:
Biochimie 2015 v.110 pp. 45-51
ISSN:
0300-9084
Subject:
Myceliophthora thermophila,   etc   ; Penicillium; Talaromyces verruculosus; Trichoderma reesei; amylopectin; amylose; crystal structure; glucan 1,4-alpha-glucosidase; glucose; hydrolysis;     Show all 10 Subjects
Abstract:
... Two glucoamylases, a recombinant enzyme from Penicillium verruculosum (PvGla) heterologously expressed in Penicillium canescens RN3-11-7 (niaD-) strain and a native glucoamylase from Myceliophthora thermophila (MtGla), were purified and their properties were studied. MtGla displayed 2–5-fold higher specific activities against soluble starch, amylose and amylopectin than PvGla. MtGla also provided ...
DOI:
10.1016/j.biochi.2014.12.010

5. Characteristics of Recombinant Phytase (rSt-Phy) of the Thermophilic mold Sporotrichum thermophile and its applicability in dephytinizing foods

Author:
Ranjan, Bibhuti; Singh, Bijender; Satyanarayana, T.
Source:
Applied biochemistry and biotechnology 2015 v.177 no.8 pp. 1753-1766
ISSN:
0273-2289
Subject:
Myceliophthora thermophila,   etc   ; Escherichia coli; barium; breads; genes; guanidinium; ions; magnesium; molecular weight; pepsin; phosphates; phytases; phytic acid; potassium iodide; substrate specificity; thermal stability; trypsin; urea;     Show all 18 Subjects
Abstract:
... Sporotrichum thermophile produces very low titres of phytase (St-Phy) extracellularly, which is acidstable, thermostable, and protease insensitive with broad substrate specificity, and therefore, the gene encoding phytase (St-Phy) has been cloned and expressed in E. coli. The purified recombinant phytase (rSt-Phy) has the molecular mass of 55 kDa with Kₘ and Vₘₐₓ (calcium phytate), kcₐₜ and kcₐₜ/K ...
DOI:
10.1007/s12010-015-1851-4

6. Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase

Author:
Frommhagen, Matthias; Sforza, Stefano; Westphal, Adrie H; Visser, Jaap; Hinz, Sandra W A; Koetsier, Martijn J; van Berkel, Willem J H; Gruppen, Harry; Kabel, Mirjam A
Source:
Biotechnology for biofuels 2015 v.8 no.1 pp. 101
ISSN:
1754-6834
Subject:
Myceliophthora thermophila,   etc   ; biofuels; biorefining; byproducts; cellulose; chitin; databases; endo-1,4-beta-glucanase; fungi; glucooligosaccharides; mechanism of action; monosaccharides; phytomass; starch; synergism; xylan; xylooligosaccharides;     Show all 17 Subjects
Abstract:
... BACKGROUND: Many agricultural and industrial food by-products are rich in cellulose and xylan. Their enzymatic degradation into monosaccharides is seen as a basis for the production of biofuels and bio-based chemicals. Lytic polysaccharide monooxygenases (LPMOs) constitute a group of recently discovered enzymes, classified as the auxiliary activity subgroups AA9, AA10, AA11 and AA13 in the CAZy da ...
DOI:
10.1186/s13068-015-0284-1
PubMed:
26185526
PubMed Central:
PMC4504452

7. Enhancing xylanase production in the thermophilic fungus Myceliophthora thermophila by homologous overexpression of Mtxyr1

Author:
Wang, Juan; Wu, Yaning; Gong, Yanfen; Yu, Shaowen; Liu, Gang
Source:
Journal of industrial microbiology & biotechnology 2015 v.42 no.9 pp. 1233-1241
ISSN:
1367-5435
Subject:
Myceliophthora thermophila,   etc   ; Trichoderma reesei; endo-1,4-beta-glucanase; gene expression; genes; glycosides; mass spectrometry; messenger RNA; pyruvate decarboxylase; thermophilic fungi; xylanases;     Show all 11 Subjects
Abstract:
... The xylanase regulator 1 protein in Myceliophthora thermophila ATCC42464 (MtXyr1) is 60 % homologous with that of Trichoderma reesei. However, MtXyr1’s regulatory role on cellulolytic and xylanolytic genes in M. thermophila is unknown. Herein, MtXyr1 was overexpressed under the control of the MtPpdc (pyruvate decarboxylase) promoter. Compared with the wild type, the extracellular xylanase activiti ...
DOI:
10.1007/s10295-015-1628-3

8. Heterologous Expression and Characterization of a GH3 β-Glucosidase from Thermophilic Fungi Myceliophthora thermophila in Pichia pastoris

Author:
Zhao, Junqi; Guo, Chao; Tian, Chaoguang; Ma, Yanhe
Source:
Applied biochemistry and biotechnology 2015 v.177 no.2 pp. 511-527
ISSN:
0273-2289
Subject:
Myceliophthora thermophila,   etc   ; thermal stability; molecular models; Komagataella pastoris; cellobiose; nucleotides; amino acids; thermophilic fungi; genes; pH; enzyme substrates; heterologous gene expression; glycosides; beta-glucosidase; gentiobiose;     Show all 15 Subjects
Abstract:
... A novel β-glucosidase of glycoside hydrolase (GH) family 3 from Myceliophthora thermophila (mtbgl3b) was successfully expressed in Pichia pastoris. The full-length gene consists of 2613 bp nucleotides encoding a protein of 870 amino acids. MtBgl3b showed maximum activity at pH 5.0 and remained more than 70 % relative activity at 3.5–6.0. The enzyme displayed the highest activity at 60 °C and kept ...
DOI:
10.1007/s12010-015-1759-z

9. Characterization and application of a novel class II thermophilic peroxidase from Myceliophthora thermophila in biosynthesis of polycatechol

Author:
Zerva, Anastasia; Christakopoulos, Paul; Topakas, Evangelos
Source:
Enzyme and microbial technology 2015 v.75-76 pp. 49-56
ISSN:
0141-0229
Subject:
Myceliophthora thermophila,   etc   ; thermal stability; alcohol oxidase; biosynthesis; substrate specificity; Komagataella pastoris; hydrogen peroxide; DNA; polymerization; yeasts; thermophilic fungi; catechol; temperature; enzyme activity; peroxidase;     Show all 15 Subjects
Abstract:
... A peroxidase from the thermophilic fungus Myceliophthora thermophila that belongs to ascomycete Class II based on PeroxiBase classification was functionally expressed in methylotrophic yeast Pichia pastoris. The putative peroxidase from the genomic DNA was successfully cloned in P. pastoris X-33 under the transcriptional control of the alcohol oxidase (AOX1) promoter. The heterologous production w ...
DOI:
10.1016/j.enzmictec.2015.04.012

10. Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification

Author:
Cassia Pereira, J.; Paganini Marques, N.; Rodrigues, A.; Brito de Oliveira, T.; Boscolo, M.; Silva, R.; Gomes, E.; Bocchini Martins, D.A.
Source:
Journal of applied microbiology 2015 v.118 no.4 pp. 928-939
ISSN:
1364-5072
Subject:
Myceliophthora thermophila,   etc   ; beta-glucosidase; cellulose; endo-1,4-beta-glucanase; glucose; glycerol; heat tolerance; lignocellulose; saccharification; solid state fermentation; sugarcane bagasse; thermophilic fungi; xylanases; xylose;     Show all 14 Subjects
Abstract:
... AIMS: To obtain new cellulases and xylanases from thermophilic fungi; evaluate their potential for sugarcane bagasse saccharification. METHODS AND RESULTS: Thirty‐two heat‐tolerant fungi were isolated from the environment, identified (morphological/molecular tools) and the production of the enzymes was evaluated by solid state fermentation using lignocellulosic materials as substrates. Myceliophth ...
DOI:
10.1111/jam.12757

11. In-Depth 2D NMR Study of Lignin Modification During Pretreatment of Eucalyptus Wood with Laccase and Mediators

Author:
Rico, Alejandro; Rencoret, Jorge; del Río, José C.; Martínez, Angel T.; Gutiérrez, Ana
Source:
BioEnergy research 2015 v.8 no.1 pp. 211-230
ISSN:
1939-1234
Subject:
Myceliophthora thermophila,   etc   ; Eucalyptus; Pycnoporus; delignification; enzymatic hydrolysis; feedstocks; filtrates; gels; glucose; laccase; lignin; nuclear magnetic resonance spectroscopy; redox potential; saccharification; wood;     Show all 15 Subjects
Abstract:
... Eucalypt feedstock was pretreated with Myceliophthora thermophila laccase and methyl syringate, as mediator, in a multistage sequence consisting of successive enzymatic and alkaline peroxide stages, directly on the ground wood. Lignin modification was studied by two-dimensional (2D) nuclear magnetic resonance (NMR) of wood (at the gel state) after each stage of the sequence. Decrease of guaiacyl a ...
DOI:
10.1007/s12155-014-9505-x

12. Effect of mutations on the thermostability of Aspergillus aculeatus β-1,4-galactanase

Author:
Torpenholt, Søs; De Maria, Leonardo; Olsson, Mats H.M.; Christensen, Lars H.; Skjøt, Michael; Westh, Peter; Jensen, Jan H.; Lo Leggio, Leila
Source:
Computational and Structural Biotechnology Journal 2015 v.13 pp. 256-264
ISSN:
2001-0370
Subject:
Myceliophthora thermophila,   etc   ; Aspergillus aculeatus; biotechnology; differential scanning calorimetry; enzyme inactivation; half life; heat inactivation; melting point; mutants; mutation; prediction; thermal stability; thermophilic microorganisms;     Show all 13 Subjects
Abstract:
... New variants of β-1,4-galactanase from the mesophilic organism Aspergillus aculeatus were designed using the structure of β-1,4-galactanase from the thermophile organism Myceliophthora thermophila as a template. Some of the variants were generated using PROPKA 3.0, a validated pKa prediction tool, to test its usefulness as an enzyme design tool. The PROPKA designed variants were D182N and S185D/Q1 ...
DOI:
10.1016/j.csbj.2015.03.010
PubMed:
25941560
PubMed Central:
PMC4412966

13. Hygroscopic properties of solid agro-industrial by-products used in solid-state fermentation

Author:
Casciatori, Fernanda Perpétua; Laurentino, Carmem Lúcia; Zanelato, Alex Izuka; Thoméo, João Cláudio
Source:
Industrial crops and products 2015 v.64 pp. 114-123
ISSN:
0926-6690
Subject:
Myceliophthora thermophila,   etc   ; bioreactors; culture flasks; endo-1,4-beta-glucanase; fungi; gravimetry; heat; hygroscopicity; mass transfer; models; solid state fermentation; sorption isotherms; sugarcane bagasse; water activity; water content; water temperature; wheat bran;     Show all 17 Subjects
Abstract:
... One of the main drawbacks in operating solid-state fermentation (SSF) bioreactors is the reduction in moisture content (MC) of the solid phase due to microbiological and physicochemical issues. Therefore, heat and mass transfer models require hygroscopic information concerning the solid substrate. This contribution addresses the study of the sorption isotherms of sugarcane bagasse (SCB) and wheat ...
DOI:
10.1016/j.indcrop.2014.11.034