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- Niwa, Hidekazu, et al. Show all 7 Authors
- Journal of virological methods 2015 v.215-216 pp. 13-16
- Coronavirinae; RNA; equipment; feces; horses; monitoring; pathogens; quantitative polymerase chain reaction; rapid methods; reverse transcriptase polymerase chain reaction; reverse transcription loop-mediated isothermal amplification
- ... A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of equine coronavirus (ECoV). This assay was conducted at 60°C for 40min. Specificity of the RT-LAMP assay was confirmed using several equine intestinal and respiratory pathogens in addition to ECoV. The novel assay failed to cross-react with the other pathogens tested, suggesting i ...