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You searched for: Publication year rev 7986-2014 Remove constraint Publication year rev: 7986-2014 Subject term DNA Remove constraint Subject term: DNA
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1. (E)-2,4-Bis(p-hydroxyphenyl)-2-butenal enhanced TRAIL-induced apoptosis in ovarian cancer cells through downregulation of NF-κB/STAT3 pathway

Author:
Seung Hee Cho; Mi Hee Park; Hee Peom Lee; Myong Ki Back; Ha Chang Sung; Hee Won Chang; Joo Hwan Kim; Heon-Sang Jeong; Sang Bae Han; Jin Tae Hong
Source:
Archives of pharmacal research 2014 v.37 no.5 pp. 652-661
ISSN:
0253-6269
Subject:
transcription factor NF-kappa B; Maillard reaction; humans; non-specific protein-tyrosine kinase; prognosis; DNA; proteolysis; signal transduction; ovarian neoplasms; antineoplastic agents; neoplasm cells; phosphorylation; animal ovaries; apoptosis; caspase-3
Abstract:
... Ovarian cancer is a cancerous growth arising from the ovary and with poor prognosis that usually have resistant to all currently available treatments. Whether (E)-2,4-bis(p-hydroxyphenyl)-2-butenal (butenal) synthesized by Maillard reaction from fructose–tyrosine, has potential therapeutic activity against human ovarian cancer was investigated using two ovarian cancer cell lines (PA-1, SK-OV-3). W ...
DOI:
10.1007/s12272-013-0326-9

2. 14-Deoxy-11,12-didehydroandrographolide inhibits proliferation and induces GSH-dependent cell death of human promonocytic leukemic cells

Author:
Rahul Raghavan; Sanith Cheriyamundath; Joseph Madassery
Source:
Natural medicines 2014 v.68 no.2 pp. 387-394
ISSN:
1340-3443
Subject:
Andrographis paniculata; DNA; Western blotting; apoptosis; caspase-3; cell viability; cytotoxicity; glutathione; humans; inhibitory concentration 50; leukemia
Abstract:
... 14-Deoxy-11,12-didehydroandrographolide (AND2), an analogue of andrographolide, showed more potent cytotoxicity against human promonocytic leukemia (THP-1) cells than adherent cancer cell lines. In this study AND2 was isolated from the plant Andrographis paniculata and it was characterized. The antiproliferative effect of AND2 on both adherent (PC-3 and MDAMB) and non-adherent (THP-1 and Jurkat) c ...
DOI:
10.1007/s11418-014-0815-2
PubMed:
24458985

3. 16S rRNA Gene Pyrosequencing Reveals Shift in Patient Faecal Microbiota During High-Dose Chemotherapy as Conditioning Regimen for Bone Marrow Transplantation

Author:
Emmanuel Montassier; Eric Batard; Sébastien Massart; Thomas Gastinne; Thomas Carton; Jocelyne Caillon; Sophie Le Fresne; Nathalie Caroff; Jean Benoit Hardouin; Philippe Moreau; Gilles Potel; Françoise Le Vacon; Marie France de La Cochetière
Source:
Microbial ecology 2014 v.67 no.3 pp. 690-699
ISSN:
0095-3628
Subject:
risk; intestinal microorganisms; sequence analysis; drug therapy; ribosomal RNA; DNA primers; DNA; polymerase chain reaction; high performance liquid chromatography; feces; bone marrow transplant; genes; species diversity; patients; Escherichia; adverse effects
Abstract:
... Gastrointestinal disturbances are a side-effect frequently associated with haematological malignancies due to the intensive cytotoxic treatment given in connection with bone marrow transplantation (BMT). However, intestinal microbiota changes during chemotherapy remain poorly described, probably due to the use of culture-based and low-resolution molecular methods in previous studies. The objective ...
DOI:
10.1007/s00248-013-0355-4
PubMed:
24402367

4. 1H-Cyclopenta[b]benzofuran Lignans from Aglaia Species Inhibit Cell Proliferation and Alter Cell Cycle Distribution in Human Monocytic Leukemia Cell Lines

Author:
Frank I. Bohnenstengel; Klaus G. Steube; Corinna Meyer; Hilmar Quentmeier; Bambang W. Nugroho; Peter Proksch
Source:
Zeitschrift für Naturforschung 2014 v.54 no.12 pp. 1075-1083
ISSN:
1865-7125
Subject:
Aglaia; DNA; actinomycin D; active ingredients; cell cycle; cell growth; cell proliferation; cycloheximide; humans; lignans; methylation; neoplasm cells; protein synthesis; sulfates; thymidine; tritium; vinblastine; Vietnam
Abstract:
... Thirteen naturally occurring 1H-cyclopenta[b]benzofuran lignans of the rocaglamide type as well as one naturally occurring aglain congener all of them isolated from three Aglaia species (Aglaia duperreana, A. oligophylla and A. spectabilis) collected in Vietnam were studied for their antiproliferative effects using the human monocytic leukemia cell lines MONO-MAC-1 and MONO-MAC-6. Only rocaglamide ...
DOI:
10.1515/znc-1999-1212

5. 2,4-Dichlorophenol induces global DNA hypermethylation through the increase of S-adenosylmethionine and the upregulation of DNMTs mRNA in the liver of goldfish Carassius auratus

Author:
Xiaoning Zhang; Hui Li; Qian Qiu; Yongmei Qi; Dejun Huang; Yingmei Zhang
Source:
Comparative Biochemistry and Physiology, Part C 2014 v.160 pp. 54-59
ISSN:
1532-0456
Subject:
2,4-dichlorophenol; Carassius auratus; DNA; DNA methylation; S-adenosylmethionine; United States Environmental Protection Agency; benzene; ethidium; gene expression; gene expression regulation; genomic islands; goldfish; high performance liquid chromatography; liver; messenger RNA; phenol; pollutants; quantitative polymerase chain reaction; signal transduction; sodium selenite; stress response; toxicity
Abstract:
... Altered DNA methylation is associated with changes in gene expression, signal transduction and stress response after exposure to a wide range of exogenous compounds, and abnormal methylation is a major toxic effect induced by chemicals such as benzene and phenols. 2,4-Dichlorophenol (2,4-DCP), a derivative of phenol, has been classified as a priority pollutant by the US EPA due to its toxic effect ...
DOI:
10.1016/j.cbpc.2013.11.008

6. 2matrix: A Utility for Indel Coding and Phylogenetic Matrix Concatenation

Author:
Salinas Nelson R.; Little Damon P.
Source:
Applications in plant sciences 2014 v.2 no.1 pp. -
ISSN:
2168-0450
Subject:
DNA; amino acid sequences; data collection; phylogeny; sequence alignment
Abstract:
... Premise of the study: Phylogenetic analysis of DNA and amino acid sequences requires the creation of files formatted specifically for each analysis package. Programs currently available cannot simultaneously code inferred insertion/deletion (indel) events in sequence alignments and concatenate data sets. Methods and Results: A novel Perl script, 2matrix, was created to concatenate matrices of non- ...
DOI:
10.3732/apps.1300083
PubMed:
25202595
PubMed Central:
PMC4123383

7. 2’-Hydroxyflavanone Induces Apoptosis of Human Osteosarcoma 143 B Cells by Activating the Extrinsic TRAIL- and Intrinsic Mitochondria-Mediated Pathways

Author:
Ko-Hsiu Lu; Pei-Ni Chen; Ko-Huang Lue; Ming-Tsung Lai; Meng-Shian Lin; Yih-Shou Hsieh; Shu-Chen Chu
Source:
Nutrition and cancer 2014 v.66 no.4 pp. 625-635
ISSN:
1532-7914
Subject:
B-lymphocytes; DNA; apoptosis; cell viability; cytochrome c; death; flavanones; flow cytometry; humans; in vivo studies; membrane potential; mitochondrial membrane; nutrition; osteosarcoma
Abstract:
... Flavanones demonstrate a propensity to antiproliferation and induce apoptosis of malignant cells. Among the 4 flavanones under study, 2′-hydroxyflavanone exhibited the greatest potency to reduce the cell viability of 143 B cells in 4 osteosarcoma cells. Flow cytometry analysis showed that 2′-hydroxyflavanone increased the hypodiploid cells in the sub-G1 phase but resulted in the reduced DNA conten ...
DOI:
10.1080/01635581.2014.894100

8. 2′-Deoxyuridine conjugated with a reactive monobenzocyclooctyne as a DNA building block for copper-free click-type postsynthetic modification of DNA

Author:
Claudia Stubinitzky; Gergely B. Cserép; Effi Bätzner; Péter Kele; Hans-Achim Wagenknecht
Source:
Chemical communications 2014 v.50 no.76 pp. 11218-11221
ISSN:
1364-548X
Subject:
DNA; azides; chemical reactions; fluorescence
Abstract:
... The carboxymethylmonobenzocyclooctyne group attached to the 5-position of a 2′-deoxyuridine in DNA allows rapid and efficient copper-free postsynthetic modification as demonstrated with a far-red emitting fluorescent azide probe. Upon labeling strong fluorescence intensity enhancement is observed. ...
DOI:
10.1039/c4cc02855d

9. 2′-O-methyl nucleotide modified DNA substrates influence the cleavage efficiencies of BamHI and BglII

Author:
Zhaoxue Tong; Bin Zhao; Guojie Zhao; Hong Shang; Yifu Guan
Source:
Journal of biosciences 2014 v.39 no.4 pp. 621-630
ISSN:
0250-5991
Subject:
DNA; enzyme activity; equations; nucleotides; sugars; synthetic biology
Abstract:
... Induction of endonucleolytic DNA cleavage is an essential event that links the initiating stimuli to the final effects of cells. The cleavage efficiency and thus the final yield could be affected by many factors, including structures of DNA substrates, composite structures of enzymes–substrates or enzymes–nucleic analogs and so on. However, it is not clear whether a nucleotide derivative-substitut ...
DOI:
10.1007/s12038-014-9466-4
PubMed:
25116617

10. 3,5,7,3′,4′-Pentamethoxyflavone, a quercetin derivative protects DNA from oxidative challenges: Potential mechanism of action

Author:
Rekha Jakhar; Souren Paul; Young Rong Park; Jaehong Han; Sun Chul Kang
Source:
Journal of photochemistry and photobiology 2014 v.131 pp. 96-103
ISSN:
1011-1344
Subject:
DNA; antioxidants; cytotoxicity; free radicals; macrophages; mechanism of action; neoplasms; protective effect; quercetin; therapeutics
Abstract:
... DNA protection is one of the most important strategies in cancer therapy. Since quercetin and its derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3′,4′-pentamethoxyflavone (PMF), a quercetin derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor a ...
DOI:
10.1016/j.jphotobiol.2014.01.003

11. 3-O-β-ᴅ-Galactopyranoside of Quercetin as an Active Principle from High Altitude Podophyllum hexandrum and Evaluation of its Radioprotective Properties

Author:
Raman Chawla; Rajesh Arora; Ravinder K. Sagar; Shikha Singh; Satish C. Puri; Raj Kumara; Surender Singh; Ashok K. Sharma; Jagdish Prasad; Haider A. Khan; Rakesh Kumar Sharma; Kanaya Lal Dhar; Michael Spiteller; Ghulam Nabi Qazi
Source:
Zeitschrift für Naturforschung 2014 v.60 no.9-10 pp. 728-738
ISSN:
0939-5075
Subject:
DNA; Podophyllum hexandrum; acid hydrolysis; altitude; antioxidant activity; bovine serum albumin; brain; gamma radiation; iron; kidneys; lignans; linoleic acid; lipid peroxidation; liquid chromatography; mass spectrometry; models; nuclear magnetic resonance spectroscopy; quercetin; radioprotective effect
Abstract:
... The aqueous-ethanolic extract (AEE) of high altitude Podophyllum hexandrum has earlier been reported to render a radioprotective effect against lethal gamma radiation in in vitro model. AEE has also been reported to possess metal chelating and DNA protecting properties. The present study was undertaken to isolate and characterize the bioactive principle present in AEE and investigate its role in r ...
DOI:
10.1515/znc-2005-9-1012

12. 3-deoxysilybin exerts anti-inflammatory effects by suppressing NF-κB activation in lipopolysaccharide-stimulated RAW264.7 macrophages

Author:
Byoung Ok Cho; Yangkang So; Chang Hyun Jin; Bo Mi Nam; Sung-Tae Yee; Il Yun Jeong
Source:
Bioscience, biotechnology, and biochemistry 2014 v.78 no.12 pp. 2051-2058
ISSN:
1347-6947
Subject:
DNA; Silybum marianum; anti-inflammatory activity; biotechnology; flavonoids; interleukin-6; lipopolysaccharides; macrophages; transcriptional activation
Abstract:
... 3-deoxysilybin (3-DS), also known as (–)-isosilandrin A, is a natural flavonoid of Silybum marianum. This study was designed to investigate the anti-inflammatory effect and the underlying molecular mechanisms of 3-DS in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. 3-DS dose-dependently inhibited the production of NO and the expression of iNOS in LPS-stimulated RAW264.7 macrophages. 3- ...
DOI:
10.1080/09168451.2014.948377

13. 3D Structural Integrity and Interactions of Single-Stranded Protein-Binding DNA in a Functionalized Nanopore

Author:
Mahmood Mohammed Arif I.; Ali Waqas; Adnan Ashfaq; Iqbal Samir M.
Source:
Journal of physical chemistry 2014 v.118 no.22 pp. 5799-5806
ISSN:
1520-5207
Subject:
DNA; biomarkers; coatings; electric field; energy; ligands; models; molecular dynamics; nanopores; neoplasms; nucleotide sequences; oligonucleotides; protein binding; protein transport; statistics; thrombin
Abstract:
... Biomarker-binding nucleotide sequences, like aptamers, have gained recent attention in cancer cell isolation and detection works. Self-assembly and 3D conformation of aptamers enable them to selectively capture and bind diseased cells and related biomarkers. One mode of utilizing such an extraordinary selective property of the aptamers is by grafting these in nanopores. Coating the inside walls of ...
DOI:
10.1021/jp411820w

14. 3D Trajectories Adopted by Coding and Regulatory DNA Elements: First-Passage Times for Genomic Interactions

Author:
Joseph S. Lucas; Yaojun Zhang; Olga K. Dudko; Cornelis Murre
Source:
Cell 2014 v.158 pp. 339-352
ISSN:
0092-8674
Subject:
B-lymphocytes; DNA; antigens; binding sites; chromatin; immunoglobulins; models; probability; regulatory sequences; topology; viscoelasticity
Abstract:
... During B lymphocyte development, immunoglobulin heavy-chain variable (VH), diversity (DH), and joining (JH) segments assemble to generate a diverse antigen receptor repertoire. Here, we have marked the distal VH and DH-JH-Eμ regions with Tet-operator binding sites and traced their 3D trajectories in pro-B cells transduced with a retrovirus encoding Tet-repressor-EGFP. We found that these elements ...
DOI:
10.1016/j.cell.2014.05.036
PubMed:
24998931
PubMed Central:
PMC4113018

15. 3D molecular modeling and evolutionary study of the Trypanosoma brucei DNA Topoisomerase IB, as a new emerging pharmacological target

Author:
Dimitrios Vlachakis; Athanasia Pavlopoulou; Maria G. Roubelakis; Christos Feidakis; Nikolaos P. Anagnou; Sophia Kossida
Source:
Genomics 2014 v.103 pp. 107-113
ISSN:
0888-7543
Subject:
DNA; DNA topoisomerase; Trypanosoma brucei; amino acids; drugs; genome; molecular models; pharmacology; phylogeny
Abstract:
... In the present study, an outline is proposed that may lead to specific drug design targeting of the Trypanosoma brucei DNA Topoisomerase IB. In this direction, an unequivocally specific platform was designed for the development of selective modulators. The designed platform is focused on the unique structural and catalytic features of the enzyme. Extensive phylogenetic analysis based on all availa ...
DOI:
10.1016/j.ygeno.2013.11.008

16. 4,5′-Dimethylangelicin, a New Very Active Monofunctional Furoeoumarin

Author:
F. Bordon; F. Baccichetti; F. Carlassare
Source:
Zeitschrift für Naturforschung 2014 v.33 no.3-4 pp. 296-298
ISSN:
1865-7125
Subject:
DNA; DNA replication; carcinoma; crosslinking; irradiation; neoplasm cells; photosensitivity
Abstract:
... 4,5′-dimethylangelicin is a new angular furocoumarin that by irradiation at 365 nm shows a very high photo­ reactivity towards DNA in vivo, without forming any cross-linkage; by photosensitization of Ehrlich ascites tumor cells a strong inhibition of DNA synthesis was observed. ...
DOI:
10.1515/znc-1978-3-423

17. 4′-C-[(4-Trifluoromethyl-1H-1,2,3-triazol-1-yl)methyl]thymidine as a Sensitive 19F NMR Sensor for the Detection of Oligonucleotide Secondary Structures

Author:
Granqvist Lotta; Virta Pasi
Source:
Journal of organic chemistry 2014 v.79 no.8 pp. 3529-3536
ISSN:
1520-6904
Subject:
DNA; Human immunodeficiency virus 1; RNA; denaturation; models; moieties; monitoring; nuclear magnetic resonance spectroscopy; oligonucleotides; organic chemistry; thymidine; ultraviolet-visible spectroscopy
Abstract:
... 4′-C-[(4-Trifluoromethyl-1H-1,2,3-triazol-1-yl)methyl]thymidine was synthesized and incorporated as a phosphoramidite into oligonucleotide sequences. Its applicability as a sensor for the ¹⁹F NMR spectroscopic detection of DNA and RNA secondary structures was demonstrated. On DNA, the ¹⁹F NMR measurements were focused on monitoring of duplex–triplex conversion, for which this fluorine-labeled 2′-d ...
DOI:
10.1021/jo500326j

18. 5,6-Dihydropyrimidine Peroxyl Radical Reactivity in DNA

Author:
San Pedro Joanna Maria N.; Greenberg Marc M.
Source:
Journal of the American Chemical Society 2014 v.136 no.10 pp. 3928-3936
ISSN:
1520-5126
Subject:
DNA; electron transfer; oligonucleotides; oxidative stress; oxygen; superoxide anion
Abstract:
... Nucleobase radicals are a major family of reactive species produced in DNA as a result of oxidative stress. Two such radicals, 5-hydroxy-5,6-dihydrothymidin-6-yl radical (1) and 5,6-dihydrouridin-6-yl radical (5), were independently generated within chemically synthesized oligonucleotides from photochemical precursors. Neither nucleobase radical produces direct strand breaks or alkali-labile lesio ...
DOI:
10.1021/ja412562p
PubMed:
24579910
PubMed Central:
PMC3980663

19. 5-(2-Carboxyethenyl) isatin derivative induces G2/M cell cycle arrest and apoptosis in human leukemia K562 cells

Author:
Yao Zhou; Hong-Ye Zhao; Kai-Lin Han; Yao Yang; Bin-Bin Song; Qian-Nan Guo; Zhen-Chuan Fan; Yong-Min Zhang; Yu-Ou Teng; Peng Yu
Source:
Biochemical and biophysical research communications 2014 v.450 pp. 1650-1655
ISSN:
0006-291X
Subject:
DNA; antineoplastic agents; apoptosis; caspase-3; cell cycle checkpoints; cyclins; cytotoxicity; flow cytometry; humans; inhibitory concentration 50; leukemia; membrane potential; mitochondria; mitochondrial membrane; staining
Abstract:
... Our previous study successfully identified that the novel isatin derivative (E)-methyl 3-(1-(4-methoxybenzyl)-2,3-dioxoindolin-5-yl) acrylate (HKL 2H) acts as an anticancer agent at an inhibitory concentration (IC50) level of 3nM. In this study, the molecular mechanism how HKL 2H induces cytotoxic activity in the human chronic myelogenous leukemia K562 cells was investigated. Flow cytometric analy ...
DOI:
10.1016/j.bbrc.2014.07.053

20. 5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels

Author:
Rachelle E. Irwin; Avinash Thakur; Karla M. O' Neill; Colum P. Walsh
Source:
Genomics 2014 v.104 pp. 383-392
ISSN:
0888-7543
Subject:
DNA; adults; brain; embryonic stem cells; fibroblasts; gene silencing; genes; inheritance (genetics); methylation; methyltransferases; neurons; transcription (genetics)
Abstract:
... We recently identified a class of neuronal gene inheriting high levels of intragenic methylation from the mother and maintaining this through later development. We show here that these genes are implicated in basic neuronal functions such as post-synaptic signalling, rather than neuronal development and inherit high levels of 5mC, but not 5hmC, from the mother. 5mC is distributed across the gene b ...
DOI:
10.1016/j.ygeno.2014.08.013

21. 5-Hydroxymethylcytosine: A stable or transient DNA modification?

Author:
Maria A. Hahn; Piroska E. Szabó; Gerd P. Pfeifer
Source:
Genomics 2014 v.104 pp. 314-323
ISSN:
0888-7543
Subject:
DNA; DNA methylation; DNA repair; DNA replication; brain; chromatin; developmental stages; epigenetics; methyltransferases; oxidation; transcription (genetics)
Abstract:
... The DNA base 5-hydroxymethylcytosine (5hmC) is produced by enzymatic oxidation of 5-methylcytosine (5mC) by 5mC oxidases (the Tet proteins). Since 5hmC is recognized poorly by DNA methyltransferases, DNA methylation may be lost at 5hmC sites during DNA replication. In addition, 5hmC can be oxidized further by Tet proteins and converted to 5-formylcytosine and 5-carboxylcytosine, two bases that can ...
DOI:
10.1016/j.ygeno.2014.08.015
PubMed:
25181633
PubMed Central:
PMC4252803

22. 5-Methylcytosin-Gehalt in Rattenerythroleukämie-Zellen nach Induktion mit Dimethylsulfoxid / 5-Methylcytosine Content in Erythroleucemic Cells of Rats after Induction with Dimethylsulfoxid

Author:
R. Baur; N. Kluge; H. Kröger; H. Wohlert
Source:
Zeitschrift für Naturforschung 2014 v.37 no.9 pp. 812-817
ISSN:
0939-5075
Subject:
DNA; cell differentiation; dimethyl sulfoxide; high performance liquid chromatography; neoplasm cells; rats; restriction endonucleases
Abstract:
... Erythroleucemic cells of rats were induced by DMSO for the terminal erythroid differentia­tion. The base composition and the 5MC content of the DNA were analyzed by HPLC and a significant difference between non-induced and induced cells was not found. The analysis of labelled DNA by the restriction enzymes Hha I, Hpa II, and Msp I did not result in a different pattern before and after the inductio ...
DOI:
10.1515/znc-1982-0913

23. 5-Methylcytosine Recognition by Arabidopsis thaliana DNA Glycosylases DEMETER and DML3

Author:
Brooks Sonja C.; Fischer Robert L.; Huh Jin Hoe; Eichman Brandt F.
Source:
Biochemistry 2014 v.53 no.15 pp. 2525-2532
ISSN:
1520-4995
Subject:
Arabidopsis thaliana; DNA; DNA methylation; X chromosome; active sites; animals; cytosine; gene expression; genes; glycosylases; models; transposons
Abstract:
... Methylation of cytosine to 5-methylcytosine (5mC) is important for gene expression, gene imprinting, X-chromosome inactivation, and transposon silencing. Active demethylation in animals is believed to proceed by DNA glycosylase removal of deaminated or oxidized 5mC. In plants, 5mC is removed from the genome directly by the DEMETER (DME) family of DNA glycosylases. Arabidopsis thaliana DME excises ...
DOI:
10.1021/bi5002294
PubMed:
24678721
PubMed Central:
PMC4004242

24. 5mC Oxidation by Tet2 Modulates Enhancer Activity and Timing of Transcriptome Reprogramming during Differentiation

Author:
Gary C. Hon; Chun-Xiao Song; Tingting Du; Fulai Jin; Siddarth Selvaraj; Ah Young Lee; Chia-an Yen; Zhen Ye; Shi-Qing Mao; Bang-An Wang; Samantha Kuan; Lee E. Edsall; Boxuan Simen Zhao; Guo-Liang Xu; Chuan He; Bing Ren
Source:
Molecular cell 2014 v.56 pp. 286-297
ISSN:
1097-2765
Subject:
DNA; DNA methylation; cell differentiation; epigenetics; gene induction; genome; mammals; oxidation; proteins; transcriptome
Abstract:
... In mammals, cytosine methylation (5mC) is widely distributed throughout the genome but is notably depleted from active promoters and enhancers. While the role of DNA methylation in promoter silencing has been well documented, the function of this epigenetic mark at enhancers remains unclear. Recent experiments have demonstrated that enhancers are enriched for 5-hydroxymethylcytosine (5hmC), an oxi ...
DOI:
10.1016/j.molcel.2014.08.026
PubMed:
25263596
PubMed Central:
PMC4319980

25. 6,9-Dihydroxy-3(15)-caryophyllen-4,8-dione -a New Antibiotic from a Marasmius Species

Author:
Kerstin Fabian; Timm Anke; Olov Sterner
Source:
Zeitschrift für Naturforschung 2014 v.54 no.7-8 pp. 469-473
ISSN:
1865-7125
Subject:
DNA; Marasmius; antibiotics; antimicrobial properties; cattle; cytotoxicity; fermentation; leucine; proteins; sesquiterpenoids; spectroscopy; thymidine
Abstract:
... 6,9-Dihydroxy-3(15)-caryophyllen-4,8-dione (1), a new cytotoxic caryophyllane sesquiterpene, was isolated from fermentations of a tropical Marasmius species. The structure was established by spectroscopic methods. 1 exhibits strong cytotoxic effects on different cell lines, but only weak antimicrobial activities. 1 weakly inhibits the incorporation of leucine and thymidine into proteins and DNA of ...
DOI:
10.1515/znc-1999-7-802

26. 6-Thioguanine and zebularine down-regulate DNMT1 and globally demethylate canine malignant lymphoid cells

Author:
Brian K Flesner; Senthil R Kumar; Jeffrey N Bryan
Source:
BMC veterinary research 2014 v.10 no.1 pp. 290
ISSN:
1746-6148
Subject:
DNA; DNA methylation; T-lymphocytes; apoptosis; bioavailability; cell proliferation; cell viability; cytidine; dogs; gene expression regulation; human cell lines; humans; lymphoma; patients; veterinary drugs
Abstract:
... BACKGROUND: The antimetabolite 6-thioguanine (6-TG) has been used to treat both human and canine lymphoid malignancies. 6-TG has been shown to be epigenetically active as a demethylating agent in a human lymphoma cell line, causing downregulation of DNA methyltransferase 1 (DNMT1) through ubiquitin-targeted degradation. Zebularine (Zeb), a similar cytidine analog, also has demethylating activity a ...
DOI:
10.1186/s12917-014-0290-8
PubMed:
25480665
PubMed Central:
PMC4272768

27. 8 -Methoxypsoralen macht Agrobacterium tumefaciens, Kalanchoe daigremontiana und die Tumorinduktion empfindlich für langwelliges UV / 8-Methoxypsoralen Makes Agrobacterium tumefaciens, Kalanchoe daigremontiana, and the Tumor Induction Sensitive for Long Wave UV

Author:
Rolf Beiderbeck
Source:
Zeitschrift für Naturforschung 2014 v.29 no.9-10 pp. 572-576
ISSN:
1865-7125
Subject:
tissue repair; DNA; Agrobacterium radiobacter; Kalanchoe daigremontiana; methoxsalen; leaves; bacteria
Abstract:
... If Agrobacterium tumefaciens is treated with 8-methoxypsoralen (8-MP) and irradiated with long wave UV the subsequent growth of the bacteria is stopped whereas respiration continues for at least 7 hours. Treatment with 8-MP or UV alone has no effect. Also the wound healing processes in Kalanchoe leaves are inhibited by simultaneous applications of 8-MP and UV. Tumor induction on Kalanchoe leaves i ...
DOI:
10.1515/znc-1974-9-1021

28. A 2D DNA Lattice as an Ultrasensitive Detector for Beta Radiations

Author:
Dugasani Sreekantha Reddy; Kim Jang Ah; Kim Byeonghoon; Joshirao Pranav; Gnapareddy Bramaramba; Vyas Chirag; Kim Taesung; Park Sung Ha; Manchanda Vijay
Source:
ACS applied materials 2014 v.6 no.4 pp. 2974-2979
ISSN:
1944-8252
Subject:
DNA; Raman spectroscopy; detectors; energy transfer; exposure duration; medicine
Abstract:
... There is growing demand for the development of efficient ultrasensitive radiation detectors to monitor the doses administered to individuals during therapeutic nuclear medicine which is often based on radiopharmaceuticals, especially those involving beta emitters. Recently biological materials are used in sensors in the nanobio disciplines due to their abilities to detect specific target materials ...
DOI:
10.1021/am4055723

29. A B-cell targeting virus disrupts potentially protective genomic methylation patterns in lymphoid tissue by increasing global 5-hydroxymethylcytosine levels

Author:
Nick A Ciccone; William Mwangi; Alexey Ruzov; Lorraine P Smith; Colin Butter; Venugopal Nair
Source:
Veterinary research 2014 v.45 no.1 pp. 108
ISSN:
1297-9716
Subject:
B-lymphocytes; DNA; Infectious bursal disease virus; cell death; chickens; enzyme activation; enzymes; messenger RNA; methylation; mutagenicity; pathogenesis; viruses
Abstract:
... The mechanisms by which viruses modulate the immune system include changes in host genomic methylation. 5-hydroxymethylcytosine (5hmC) is the catalytic product of the Tet (Ten-11 translocation) family of enzymes and may serve as an intermediate of DNA demethylation. Recent reports suggest that 5hmC may confer consequences on cellular events including the pathogenesis of disease; in order to explor ...
DOI:
10.1186/s13567-014-0108-5
PubMed:
25338704
PubMed Central:
PMC4258027

30. A CMP-based method for tunable, cell-mediated gene delivery from collagen scaffolds

Author:
M. A. Urello; K. L. Kiick; M. O. Sullivan
Source:
Journal of materials chemistry B 2014 v.2 no.46 pp. 8174-8185
ISSN:
2050-7518
Subject:
DNA; biocompatible materials; biomimetics; blood serum; chemical treatment; collagen; gels; genes; metalloproteinases; peptides; therapeutics; transfection
Abstract:
... The use of collagen-based biomaterials in regenerative medicine has rapidly increased over the past decade. The unique structural and biochemical properties of collagen make it a particularly promising material for delivering both protein and DNA-based therapeutics. Although many collagen modification techniques have been developed, the majority of them require multi-step chemical treatments that ...
DOI:
10.1039/c4tb01435a

31. A Case of Leucocyte Chimerism (78,XX/78,XY) in a Dog with a Disorder of Sexual Development

Author:
I Szczerbal; J Nowacka‐Woszuk; W Nizanski; S Salamon; M Ochota; S Dzimira; W Atamaniuk; M Switonski
Source:
Reproduction in domestic animals 2014 v.49 no.3 pp. e31
ISSN:
0936-6768
Subject:
DNA; alleles; blood; chimerism; chromosomes; cytogenetic analysis; dogs; genetic markers; genitalia; hair follicles; microsatellite repeats; prepuce; scrotum; sexual development; somatic cells; testes
Abstract:
... A 1‐year‐old Shih Tzu dog was presented for examination because of abnormal external genitalia. A residual penis with a prepuce was located in a position typical of a male. The dog had no palpable testicles or scrotum. The ultrasound examination revealed the presence of the prostate, but the gonads remained undetectable. Cytogenetic analysis performed on chromosome preparations obtained from lymph ...
DOI:
10.1111/rda.12318
PubMed:
24735223

32. A Cell-Surface-Anchored Ratiometric Fluorescent Probe for Extracellular pH Sensing

Author:
Ke Guoliang; Zhu Zhi; Wang Wei; Zou Yuan; Guan Zhichao; Jia Shasha; Zhang Huimin; Wu Xuemeng; Yang Chaoyong James
Source:
ACS Applied Materials & Interfaces 2014 v.6 no.17 pp. 15329-15334
ISSN:
1944-8252
Subject:
DNA; cell membranes; cytotoxicity; fluorescent dyes; hydrophilicity; hydrophobic bonding; hydrophobicity; pH
Abstract:
... Accurate sensing of the extracellular pH is a very important yet challenging task in biological and clinical applications. This paper describes the development of an amphiphilic lipid–DNA molecule as a simple yet useful cell-surface-anchored ratiometric fluorescent probe for extracellular pH sensing. The lipid–DNA probe, which consists of a hydrophobic diacyllipid tail and a hydrophilic DNA strand ...
DOI:
10.1021/am503818n

33. A Chemically Competent Thiosulfuranyl Radical on the Escherichia coli Class III Ribonucleotide Reductase

Author:
Wei Yifeng; Mathies Guinevere; Yokoyama Kenichi; Chen Jiahao; Griffin Robert G.; Stubbe JoAnne
Source:
Journal of the American Chemical Society 2014 v.136 no.25 pp. 9001-9013
ISSN:
1520-5126
Subject:
Archaea; DNA; Escherichia coli; active sites; adenosine triphosphate; anaerobes; cysteine; cytidine; cytosine; electron paramagnetic resonance spectroscopy; formates; free radicals; isotopes; methionine; reducing agents; ribonucleotide reductase; thioredoxins; tritium
Abstract:
... The class III ribonucleotide reductases (RNRs) are glycyl radical (G•) enzymes that provide the balanced pool of deoxynucleotides required for DNA synthesis and repair in many facultative and obligate anaerobic bacteria and archaea. Unlike the class I and II RNRs, where reducing equivalents for the reaction are delivered by a redoxin (thioredoxin, glutaredoxin, or NrdH) via a pair of conserved act ...
DOI:
10.1021/ja5030194
PubMed:
24827372
PubMed Central:
PMC4073831

34. A Chiroptical Switch Based on DNA/Layered Double Hydroxide Ultrathin Films

Author:
Shi Wenying; Jia Yankun; Xu Simin; Li Zhixiong; Fu Yi; Wei Min; Shi Shuxian
Source:
Langmuir 2014 v.30 no.43 pp. 12916-12922
ISSN:
1520-5827
Subject:
DNA; atomic force microscopy; circular dichroism spectroscopy; fluorescence; hydrochloric acid; information storage; nanosheets; optical isomerism; scanning electron microscopy; vapors
Abstract:
... A highly oriented film was fabricated by layer-by-layer self-assembly of DNA and MgAl-layered double hydroxide nanosheets, and its application in chiroptical switch was demonstrated via intercalation and deintercalation of an achiral molecule into the DNA cavity. DNA molecules are prone to forming an ordered and dispersive state in the interlayer region of rigid layered double hydroxide (LDH) nano ...
DOI:
10.1021/la502968z

35. A Comparison of Cell Wall Disruption Techniques for the Isolation of Intracellular Metabolites from Pleurotus and Lepista sp.

Author:
Rilka M. Taskova; Holger Zorn; Ulrich Krings; Henning Bouws; Ralf G. Berger
Source:
Zeitschrift für Naturforschung 2014 v.61 no.5-6 pp. 347-350
ISSN:
1865-7125
Subject:
DNA; Lepista; Pleurotus sapidus; RNA; cell walls; enzymes; fungi; grinding; homogenization; milling; mycelium; nitrogen; secondary metabolites
Abstract:
... Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes. Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular me ...
DOI:
10.1515/znc-2006-5-608

36. A Conformational Ensemble Derived Using NMR Methyl Chemical Shifts Reveals a Mechanical Clamping Transition That Gates the Binding of the HU Protein to DNA

Author:
Kannan Arvind; Camilloni Carlo; Sahakyan Aleksandr B.; Cavalli Andrea; Vendruscolo Michele
Source:
Journal of the American Chemical Society 2014 v.136 no.6 pp. 2204-2207
ISSN:
1520-5126
Subject:
DNA; Gibbs free energy; molecular dynamics; nuclear magnetic resonance spectroscopy; prediction; proteins
Abstract:
... Recent improvements in the accuracy of structure-based methods for the prediction of nuclear magnetic resonance chemical shifts have inspired numerous approaches for determining the secondary and tertiary structures of proteins. Such advances also suggest the possibility of using chemical shifts to characterize the conformational fluctuations of these molecules. Here we describe a method of using ...
DOI:
10.1021/ja4105396

37. A DEK Domain-Containing Protein Modulates Chromatin Structure and Function in Arabidopsis

Author:
Sascha Waidmann; Branislav Kusenda; Juliane Mayerhofer; Karl Mechtler; Claudia Jonak
Source:
plant cell 2014 v.26 no.11 pp. 4328-4344
ISSN:
1040-4651
Subject:
Arabidopsis; DNA; chromatin; gene expression; stress tolerance; topology
Abstract:
... This study identifies DEK3 as a chromatin architectural protein capable of modulating DNA topology, DNA accessibility, and gene expression in Arabidopsis . Furthermore, functional levels of DEK3 are crucial for stress tolerance. ...
DOI:
10.1105/tpc.114.129254
PubMed:
25387881
PubMed Central:
PMC4277211

38. A DNA Mimic: The Structure and Mechanism of Action for the Anti-Repressor Protein AbbA

Author:
Ashley T. Tucker; Benjamin G. Bobay; Allison V. Banse; Andrew L. Olson; Erik J. Soderblom; M. Arthur Moseley; Richele J. Thompson; Kristen M. Varney; Richard Losick; John Cavanagh
Source:
Journal of Molecular Biology 2014 v.426 pp. 1911-1924
ISSN:
0022-2836
Subject:
Bacillus subtilis; DNA; DNA-binding domains; bacteria; environmental factors; genes; mechanism of action; nuclear magnetic resonance spectroscopy; phosphates
Abstract:
... Bacteria respond to adverse environmental conditions by switching on the expression of large numbers of genes that enable them to adapt to unfavorable circumstances. In Bacillus subtilis, many adaptive genes are under the negative control of the global transition state regulator, the repressor protein AbrB. Stressful conditions lead to the de-repression of genes under AbrB control. Contributing to ...
DOI:
10.1016/j.jmb.2014.02.010
PubMed:
24534728
PubMed Central:
PMC4017629

39. A DNA aptamer to c-Met inhibits cancer cell migration

Author:
R. Ueki; S. Sando
Source:
Chemical communications 2014 v.50 no.86 pp. 13131-13134
ISSN:
1364-548X
Subject:
DNA; cell movement; chemical compounds; chemical reactions; metastasis; neoplasm cells; neoplasms; oligonucleotides
Abstract:
... The HGF/c-Met pathway is known to play a key role in cancer metastasis. CLN0003_SL1, a 50-mer DNA aptamer that binds to the c-Met protein, was found to inhibit HGF-induced c-Met activation. This aptamer could suppress cancer cell motility in vitro. ...
DOI:
10.1039/c4cc06016d

40. A DNA assay for rapid discrimination between beaver species as a tool for alien species management

Author:
R. McEwing; C. Frosch; F. Rosell; R. Campbell-Palmer
Source:
European journal of wildlife research 2014 v.60 no.3 pp. 547-550
ISSN:
1612-4642
Subject:
Castoridae; DNA; introduced species; single nucleotide polymorphism; Eurasia; Scotland
Abstract:
... The confirmed presence of alien North American beavers in some regions of Eurasia may compete with and hinder the successful recolonisation of the native Eurasian species back to its former range. Distinguishing the two species in the field can be problematic, time consuming and expensive, thereby potentially limiting appropriate conservation actions. Here, a rapid and inexpensive genetic SNP assa ...
DOI:
10.1007/s10344-014-0803-6

41. A DNA marker assay based on high-resolution melting curve analysis for distinguishing species of the Festuca–Lolium complex

Author:
Martina Birrer; Roland Kölliker; Chloe Manzanares; Torben Asp; Bruno Studer
Source:
Molecular breeding 2014 v.34 no.2 pp. 421-429
ISSN:
1380-3743
Subject:
DNA; Lolium perenne; breeding; genetic markers; grasses; melting; nucleotide sequences; polymerase chain reaction; seeds; single nucleotide polymorphism
Abstract:
... The grass breeding industry is interested in a fast and cheap method of identifying contamination in seeds of Italian and perennial ryegrass (Lolium perenne L. and L. multiflorum Lam., respectively). This study shows that high-resolution melting curve analysis in combination with an unlabelled probe assay is an effective method of detecting single nucleotide polymorphisms (SNPs) in diverse Italian ...
DOI:
10.1007/s11032-014-0044-0

42. A DNA mini‐barcode for land plants

Author:
Damon P. Little
Source:
Molecular ecology notes 2014 v.14 no.3 pp. 437-446
ISSN:
1755-098X
Subject:
DNA; embryophytes; polymerase chain reaction; probability
Abstract:
... Small portions of the barcode region – mini‐barcodes – may be used in place of full‐length barcodes to overcome DNA degradation for samples with poor DNA preservation. 591,491,286 rbcL mini‐barcode primer combinations were electronically evaluated for PCR universality, and two novel highly universal sets of priming sites were identified. Novel and published rbcL mini‐barcode primers were evaluated ...
DOI:
10.1111/1755-0998.12194
PubMed:
24286499

43. A DNA-Directed Light-Harvesting/Reaction Center System

Author:
Dutta Palash K.; Levenberg Symon; Loskutov Andrey; Jun Daniel; Saer Rafael; Beatty J. Thomas; Lin Su; Liu Yan; Woodbury Neal W.; Yan Hao
Source:
Journal of the American Chemical Society 2014 v.136 no.47 pp. 16618-16625
ISSN:
1520-5126
Subject:
DNA; Rhodobacter sphaeroides; dyes; energy transfer; nanomaterials; photonics; photostability; spectral analysis
Abstract:
... A structurally and compositionally well-defined and spectrally tunable artificial light-harvesting system has been constructed in which multiple organic dyes attached to a three-arm-DNA nanostructure serve as an antenna conjugated to a photosynthetic reaction center isolated from Rhodobacter sphaeroides 2.4.1. The light energy absorbed by the dye molecules is transferred to the reaction center, wh ...
DOI:
10.1021/ja509018g

44. A DNA-templated synthesis of encoded small molecules by DNA self-assembly

Author:
Cheng Cao; Peng Zhao; Ze Li; Zitian Chen; Yanyi Huang; Yu Bai; Xiaoyu Li
Source:
Chemical communications 2014 v.50 no.75 pp. 10997-10999
ISSN:
1364-548X
Subject:
DNA; chemical compounds; chemical reactions
Abstract:
... We report a novel method for the synthesis of DNA-encoded libraries without the need for discrete DNA template. Reactant DNAs self-assemble to enable chemical reactions and photo-cleavage transfers the product to the DNA terminus, making it suitable for the subsequent affinity-based selection and hit deconvolution. ...
DOI:
10.1039/c4cc03380a

45. A Divergent Role of the SIRT1-TopBP1 Axis in Regulating Metabolic Checkpoint and DNA Damage Checkpoint

Author:
Tongzheng Liu; Yi-Hui Lin; Wenchuan Leng; Sung Yun Jung; Haoxing Zhang; Min Deng; Debra Evans; Yunhui Li; Kuntian Luo; Bo Qin; Jun Qin; Jian Yuan; Zhenkun Lou
Source:
Molecular cell 2014 v.56 pp. 681-695
ISSN:
1097-2765
Subject:
DNA; DNA damage; DNA replication; acetylation; genotoxicity; glucose
Abstract:
... DNA replication is executed only when cells have sufficient metabolic resources and undamaged DNA. Nutrient limitation and DNA damage cause a metabolic checkpoint and DNA damage checkpoint, respectively. Although SIRT1 activity is regulated by metabolic stress and DNA damage, its function in these stress-mediated checkpoints remains elusive. Here we report that the SIRT1-TopBP1 axis functions as a ...
DOI:
10.1016/j.molcel.2014.10.007
PubMed:
25454945
PubMed Central:
PMC4386886

46. A FIASCO-Based Approach for Detection and Diagnosis of Puccinia graminis f. sp. tritici in China

Author:
Tai-guo LIU; Xi WANG; Li GAO; Bo LIU; Wan-quan CHEN; Wen-sheng XIANG
Source:
Journal of integrative agriculture 2014 v.13 no.11 pp. 2438-2444
ISSN:
2095-3119
Subject:
DNA; DNA fragmentation; DNA primers; Puccinia graminis; Puccinia recondita; Puccinia striiformis; Triticum aestivum; amplified fragment length polymorphism; detection limit; disease control; disease outbreaks; fungi; hyphae; leaves; microbial detection; microsatellite repeats; pathogens; stem rust; wheat; China
Abstract:
... Stem or black rust of wheat, caused by the fungus Puccinia graminis Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), has historically caused severe losses to wheat (Triticum aestivum L.) production worldwide. In the Fujian and Guangdong provinces of China, six moderate-to-severe epidemics of wheat stem rust have occurred, which caused destructive losses of wheat between 1949 and 1966, although these ...
DOI:
10.1016/S2095-3119(14)60895-9

47. A FoxO1-dependent, but NRF2-independent induction of heme oxygenase-1 during muscle atrophy

Author:
Jione Kang; Mi Gyeong Jeong; Sera Oh; Eun Jung Jang; Hyo Kyeong Kim; Eun Sook Hwang
Source:
FEBS letters 2014 v.588 no.1 pp. 79-85
ISSN:
0014-5793
Subject:
DNA; heme oxygenase (biliverdin-producing); homeostasis; muscles; muscular atrophy; skeletal muscle; transcription (genetics)
Abstract:
... Skeletal muscle plays key roles in metabolic homeostasis. Loss of muscle mass, called muscle atrophy exacerbates disease-associated metabolic perturbations. In this study, we characterized the molecular functions and mechanisms underlying regulation of skeletal muscle atrophy induced by denervation. Denervation significantly increased the expression of heme oxygenase-1 (HO-1) and atrogenes in skel ...
DOI:
10.1016/j.febslet.2013.11.009

48. A G-quadruplex DNAzyme chemiluminescence aptasensor based on the target triggered DNA recycling for sensitive detection of adenosine

Author:
Zhidan Chu; Liangliang Zhang; Yong Huang; Shulin Zhao
Source:
Analytical methods 2014 v.6 no.11 pp. 3700-3705
ISSN:
1759-9679
Subject:
DNA; adenosine; analytical methods; aptasensors; chemiluminescence; detection limit; dyes; hydrogen peroxide; nanomaterials; oligonucleotides; oxidation
Abstract:
... A G-quadruplex DNAzyme chemiluminescence aptasensor based on the target triggered DNA recycling was constructed for sensitive detection of adenosine. The system involves four nucleic acid strands, namely DNA1, DNA2, H1 and H2. DNA1 is a chimeric conjugate of the adenosine aptamer strand, DNA2 is an inhibitory strand that contains complementary sequences to DNA1. Hairpin structures H1 and H2 are de ...
DOI:
10.1039/c4ay00251b

49. A G-quadruplex-based, label-free, switch-on luminescent detection assay for Ag⁺ ions based on the exonuclease III-mediated digestion of C–Ag⁺–C DNA

Author:
Modi Wang; Ka-Ho Leung; Sheng Lin; Daniel Shiu-Hin Chan; Chung-Hang Leung; Dik-Lung Ma
Source:
Journal of materials chemistry B 2014 v.2 no.38 pp. 6467-6471
ISSN:
2050-7518
Subject:
DNA; enzyme activity; enzymes; ions; iridium; luminescence; silver
Abstract:
... We report herein the ability of exonuclease III to cleave C–Ag⁺–C mismatched DNA, which was utilised for the construction of an exonuclease III-assisted, label-free, switch-on luminescent platform for Ag⁺ ions using a novel G-quadruplex-selective iridium(iii) complex. ...
DOI:
10.1039/c4tb01140f

50. A General Strategy for Label-Free Sensitive DNA Detection Based on Quantum Dot Doping

Author:
He Xuewen; Ma Nan
Source:
Analytical chemistry 2014 v.86 no.7 pp. 3676-3681
ISSN:
1520-6882
Subject:
DNA; cost effectiveness; detection limit; diagnostic techniques; fluorescence emission spectroscopy; ions; mercury; mutation; nucleic acid hybridization; photoluminescence; quantum dots
Abstract:
... Development of rapid, sensitive, and cost-effective DNA detection is of great significance to meet the growing demand of disease diagnostics. Herein, we report a new general strategy for label-free sensitive in-solution DNA detection using quantum dot (QD) doping-induced photoluminescence as a fluorogenic reporter system. The dopant mercury (Hg(II)) ions are initially sequestered in the hairpin-st ...
DOI:
10.1021/ac500590d