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- Smider, Vaughn
V., et al. Show all 9 Authors
- Journal of the American Chemical Society 2014 v.136 no.23 pp. 8411-8417
- Escherichia coli; aminoacyl tRNA ligases; crosslinking; diagnostic techniques; lysine; mutants; pH; protein-protein interactions; stop codon; therapeutics
- ... Selective covalent bond formation at a protein–protein interface potentially can be achieved by genetically introducing into a protein an appropriately “tuned” electrophilic unnatural amino acid that reacts with a native nucleophilic residue in its cognate receptor upon complex formation. We have evolved orthogonal aminoacyl-tRNA synthetase/tRNACUA pairs that genetically encode three aza-Michael a ...
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