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- Nakayasu, Chihaya, et al. Show all 7 Authors
- Fish & shellfish immunology 2014 v.39 no.2 pp. 396-400
- 3-hydroxyacyl-CoA dehydrogenase; Escherichia coli; Flavobacterium psychrophilum; H+/K+-exchanging ATPase; H-transporting ATP synthase; Plecoglossus altivelis; antibodies; antigens; bacterial cold-water disease; blood serum; fish; fluorescent antibody technique; glutamate dehydrogenase; immune response; recombinant proteins; surface proteins; vaccine development
- ... Protective efficacies of three antigenic proteins (3-hydroxyacyl-CoA dehydrogenase (HCD), ATP synthase beta subunit (atpD), and glutamate dehydrogenase (gdhA)) against Flavobacterium psychrophilum were investigated in ayu (Plecoglossus altivelis). Recombinant proteins of HCD, atpD, and gdhA were expressed in Escherichia coli BL21 cells. Ayu were then vaccinated with inactivated cells via the intra ...